FXYD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FXYD4
Entrez Gene ID	53828
Full Name	FXYD domain containing ion transport regulator 4
Synonyms	CHIF
General protein information	Preferred Names FXYD domain containing ion transport regulator 4 Names FXYD domain-containing ion transport regulator 4 channel-inducing factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q11.21
Summary	This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD4, originally named CHIF for channel-inducing factor, has been shown to modulate the properties of the Na,K-ATPase, as has FXYD2, also known as the gamma subunit of the Na,K-ATPase, and FXYD7. Transmembrane topology has been established for FXYD4 and two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. Alternatively spliced transcript variants encoding the same protein have been found.[provided by RefSeq, May 2010].
Disorder MIM:	616926

mRNA and Protein(s)

mRNA	Protein	Name
NM_173160.2	NP_775183.1	FXYD domain-containing ion transport regulator 4 precursor
NM_173160.3	NP_775183.1	FXYD domain-containing ion transport regulator 4 precursor
NM_001184963.1	NP_001171892.1	FXYD domain-containing ion transport regulator 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FXYD4	NP_775183.1
Pan troglodytes (chimpanzee)	FXYD4	XP_001155768.1
Bos taurus (cattle)	FXYD4	XP_002698877.2
Mus musculus (house mouse)	Fxyd4	NP_001166843.1
Rattus norvegicus (Norway rat)	Fxyd4	NP_071783.1
Canis lupus familiaris (dog)	FXYD4	XP_005637559.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome wide association and linkage analyses identified three loci-4q25, 17q23.2, and 10q11.21-associated with variation in leukocyte telomere length: the Long Life Family Study.
Lee JH, Cheng R, Honig LS, Feitosa M, Kammerer CM, Kang MS, Schupf N, Lin SJ, Sanders JL, Bae H, Druley T, Perls T, Christensen K, Province M, Mayeux R
Frontiers in genetics4310(2013)

The DNA sequence and comparative analysis of human chromosome 10.
Deloukas P, Earthrowl ME, Grafham DV, Rubenfield M, French L, Steward CA, Sims SK, Jones MC, Searle S, Scott C, Howe K, Hunt SE, Andrews TD, Gilbert JG, Swarbreck D, Ashurst JL, Taylor A, Battles J, Bird CP, Ainscough R, Almeida JP, Ashwell RI, Ambrose KD, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Bates K, Beasley H, Bray-Allen S, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Cahill P, Camire D, Carter NP, Chapman JC, Clark SY, Clarke G, Clee CM, Clegg S, Corby N, Coulson A, Dhami P, Dutta I, Dunn M, Faulkner L, Frankish A, Frankland JA, Garner P, Garnett J, Gribble S, Griffiths C, Grocock R, Gustafson E, Hammond S, Harley JL, Hart E, Heath PD, Ho TP, Hopkins B, Horne J, Howden PJ, Huckle E, Hynds C, Johnson C, Johnson D, Kana A, Kay M, Kimberley AM, Kershaw JK, Kokkinaki M, Laird GK, Lawlor S, Lee HM, Leongamornlert DA, Laird G, Lloyd C, Lloyd DM, Loveland J, Lovell J, McLaren S, McLay KE, McMurray A, Mashreghi-Mohammadi M, Matthews L, Milne S, Nickerson T, Nguyen M, Overton-Larty E, Palmer SA, Pearce AV, Peck AI, Pelan S, Phillimore B, Porter K, Rice CM, Rogosin A, Ross MT, Sarafidou T, Sehra HK, Shownkeen R, Skuce CD, Smith M, Standring L, Sycamore N, Tester J, Thorpe A, Torcasso W, Tracey A, Tromans A, Tsolas J, Wall M, Walsh J, Wang H, Weinstock K, West AP, Willey DL, Whitehead SL, Wilming L, Wray PW, Young L, Chen Y, Lovering RC, Moschonas NK, Siebert R, Fechtel K, Bentley D, Durbin R, Hubbard T, Doucette-Stamm L, Beck S, Smith DR, Rogers J
Nature429(6990)375-81(2004 May)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

FXYD proteins as regulators of the Na,K-ATPase in the kidney.
Sweadner KJ, Arystarkhova E, Donnet C, Wetzel RK
Annals of the New York Academy of Sciences986382-7(2003 Apr)

Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers.
Crowell KJ, Franzin CM, Koltay A, Lee S, Lucchese AM, Snyder BC, Marassi FM
Biochimica et biophysica acta1645(1)15-21(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

molecular cloning, protein expression, sequencing and NMR structure determination
Title: Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers.

CHIF can act as tissue-specific modulators of Na-K-ATPase
Title: A functional interaction between CHIF and Na-K-ATPase: implication for regulation by FXYD proteins.

The following FXYD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FXYD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04852	NM_173160.3 Latest version!	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04852	NM_001184963.1 Latest version!	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04852	NM_173160.2	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FXYD4 ( NM_173160.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_775183.1 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04852
Clone ID Related Accession (Same CDS sequence)	NM_173160.2 , NM_173160.3 , NM_001184963.1
Accession Version	NM_173160.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	FXYD domain-containing ion transport regulator 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from BC054876.1, AA759244.1 and BF507928.1. On Jul 27, 2004 this sequence version replaced NM_173160.1. Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: ERR279833.1702.1, BC054876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775183.1
CDS	333..602
Translation	MERVTLALLLLAGLTALEANDPFANKDDPFYYDWKNLQLSGLICGGLLAIAGIAAVLSGKCKCKSSQKQHSPVPEKAIPLITPGSATTC

Target ORF information:

RefSeq Version	NM_173160.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173160.2

ORF Insert Sequence:

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FXYD4 ( NM_173160.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775183.1 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04852
Clone ID Related Accession (Same CDS sequence)	NM_173160.2 , NM_173160.3 , NM_001184963.1
Accession Version	NM_173160.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	FXYD domain-containing ion transport regulator 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from AL512654.9, BC054876.1 and AA759244.1. On Nov 23, 2018 this sequence version replaced NM_173160.2. Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: ERR279833.1702.1, BC054876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000476166.6/ ENSP00000473361.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775183.1
CDS	341..610
Translation	MERVTLALLLLAGLTALEANDPFANKDDPFYYDWKNLQLSGLICGGLLAIAGIAAVLSGKCKCKSSQKQHSPVPEKAIPLITPGSATTC

Target ORF information:

RefSeq Version	NM_173160.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173160.3

ORF Insert Sequence:

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FXYD4 ( NM_001184963.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171892.1 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04852
Clone ID Related Accession (Same CDS sequence)	NM_173160.2 , NM_173160.3 , NM_001184963.1
Accession Version	NM_001184963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	FXYD domain-containing ion transport regulator 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358584.1 and BC054876.1. Transcript Variant: This variant (2) represents use of an alternate splice acceptor site in the 5' UTR, as compared to variant 1. This variant is likely to only be found in individuals with the A allele of SNP rs10899795, which is polymorphic in most populations. ##Evidence-Data-START## Transcript exon combination :: AY358584.1, ERR279849.4214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171892.1
CDS	197..466
Translation	MERVTLALLLLAGLTALEANDPFANKDDPFYYDWKNLQLSGLICGGLLAIAGIAAVLSGKCKCKSSQKQHSPVPEKAIPLITPGSATTC

Target ORF information:

RefSeq Version	NM_001184963.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184963.1

ORF Insert Sequence:

ATGGAGAGAG TGACCCTGGC CCTTCTCCTA CTGGCAGGCC TGACTGCCTT GGAAGCCAAT 
GACCCATTTG CCAATAAAGA CGATCCCTTC TACTATGACT GGAAAAACCT GCAGCTGAGC 
GGACTGATCT GCGGAGGGCT CCTGGCCATT GCTGGGATCG CGGCAGTTCT GAGTGGCAAA 
TGCAAATGCA AGAGCAGCCA GAAGCAGCAC AGTCCTGTAC CTGAGAAGGC CATCCCACTC 
ATCACTCCAG GCTCTGCCAC TACTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome wide association and linkage analyses identified three loci-4q25, 17q23.2, and 10q11.21-associated with variation in leukocyte telomere length: the Long Life Family Study.
Frontiers in genetics4310(2013)
Lee JH,Cheng R,Honig LS,Feitosa M,Kammerer CM,Kang MS,Schupf N,Lin SJ,Sanders JL,Bae H,Druley T,Perls T,Christensen K,Province M,Mayeux R

The DNA sequence and comparative analysis of human chromosome 10.
Nature429(6990)375-81(2004 May)
Deloukas P,Earthrowl ME,Grafham DV,Rubenfield M,French L,Steward CA,Sims SK,Jones MC,Searle S,Scott C,Howe K,Hunt SE,Andrews TD,Gilbert JG,Swarbreck D,Ashurst JL,Taylor A,Battles J,Bird CP,Ainscough R,Almeida JP,Ashwell RI,Ambrose KD,Babbage AK,Bagguley CL,Bailey J,Banerjee R,Bates K,Beasley H,Bray-Allen S,Brown AJ,Brown JY,Burford DC,Burrill W,Burton J,Cahill P,Camire D,Carter NP,Chapman JC,Clark SY,Clarke G,Clee CM,Clegg S,Corby N,Coulson A,Dhami P,Dutta I,Dunn M,Faulkner L,Frankish A,Frankland JA,Garner P,Garnett J,Gribble S,Griffiths C,Grocock R,Gustafson E,Hammond S,Harley JL,Hart E,Heath PD,Ho TP,Hopkins B,Horne J,Howden PJ,Huckle E,Hynds C,Johnson C,Johnson D,Kana A,Kay M,Kimberley AM,Kershaw JK,Kokkinaki M,Laird GK,Lawlor S,Lee HM,Leongamornlert DA,Laird G,Lloyd C,Lloyd DM,Loveland J,Lovell J,McLaren S,McLay KE,McMurray A,Mashreghi-Mohammadi M,Matthews L,Milne S,Nickerson T,Nguyen M,Overton-Larty E,Palmer SA,Pearce AV,Peck AI,Pelan S,Phillimore B,Porter K,Rice CM,Rogosin A,Ross MT,Sarafidou T,Sehra HK,Shownkeen R,Skuce CD,Smith M,Standring L,Sycamore N,Tester J,Thorpe A,Torcasso W,Tracey A,Tromans A,Tsolas J,Wall M,Walsh J,Wang H,Weinstock K,West AP,Willey DL,Whitehead SL,Wilming L,Wray PW,Young L,Chen Y,Lovering RC,Moschonas NK,Siebert R,Fechtel K,Bentley D,Durbin R,Hubbard T,Doucette-Stamm L,Beck S,Smith DR,Rogers J

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

FXYD proteins as regulators of the Na,K-ATPase in the kidney.
Annals of the New York Academy of Sciences986382-7(2003 Apr)
Sweadner KJ,Arystarkhova E,Donnet C,Wetzel RK

Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers.
Biochimica et biophysica acta1645(1)15-21(2003 Jan)
Crowell KJ,Franzin CM,Koltay A,Lee S,Lucchese AM,Snyder BC,Marassi FM

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FXYD4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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