MBD3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD3
Entrez Gene ID	53615
Full Name	methyl-CpG binding domain protein 3
General protein information	Preferred Names methyl-CpG binding domain protein 3 Names methyl-CpG-binding domain protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013].
Disorder MIM:	603573

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281454.1	NP_001268383.1	methyl-CpG-binding domain protein 3 isoform 2
NM_001281454.2	NP_001268383.1	methyl-CpG-binding domain protein 3 isoform 2
NM_001281453.1	NP_001268382.1	methyl-CpG-binding domain protein 3 isoform 1
NM_001281453.2	NP_001268382.1	methyl-CpG-binding domain protein 3 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MBD3	XP_868594.1
Bos taurus (cattle)	MBD3	NP_001121977.1
Rattus norvegicus (Norway rat)	Mbd3	NP_001102205.1
Homo sapiens (human)	MBD3	NP_001268382.1
Mus musculus (house mouse)	Mbd3	NP_038623.1
Xenopus tropicalis (tropical clawed frog)	mbd3	NP_001006901.1
Danio rerio (zebrafish)	mbd3a	NP_997934.1
Pan troglodytes (chimpanzee)	MBD3	XP_512241.1
Gallus gallus (chicken)	MBD3	NP_001073219.1

Gene Ontology
Bibliography

Related articles in PubMed

Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-?/Smad signalling.
Xu M, He J, Li J, Feng W, Zhou H, Wei H, Zhou M, Lu Y, Zeng J, Peng W, Du F, Gong A
British journal of cancer116(1)91-99(2017 Jan)

MBD3 inhibits formation of liver cancer stem cells.
Li R, He Q, Han S, Zhang M, Liu J, Su M, Wei S, Wang X, Shen L
Oncotarget8(4)6067-6078(2017 Jan)

Regulatory landscape and clinical implication of MBD3 in human malignant glioma.
Cui Y, Li J, Weng L, Wirbisky SE, Freeman JL, Liu J, Liu Q, Yuan X, Irudayaraj J
Oncotarget7(49)81698-81714(2016 Dec)

MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.
Cui J, Duan B, Zhao X, Chen Y, Sun S, Deng W, Zhang Y, Du J, Chen Y, Gu L
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13455-13467(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MBD3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells probably via TGF-beta/Smad signaling and may be a candidate for diagnostics and prognosis of pancreatic cancer.
Title: Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-?/Smad signalling.

Overexpression of MBD3 is associated with neoplasms.
Title: MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.

Insufficient MBD3 induced by small interfering RNA (siRNA) was found to result in a global DNA hypermethylation as well as increased methylation in the promoter CpG islands (CGIs) of a number of cell cycle related genes.
Title: Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.

This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia.
Title: Genetic association study between methyl-CpG-binding domain genes and schizophrenia among Chinese family trios.

These data suggest that MBD3, and by extension the NuRD complex, may have multiple roles in fine tuning expression for both active and silent genes, representing an important step in defining regulatory mechanisms
Title: MBD3 localizes at promoters, gene bodies and enhancers of active genes.

The following MBD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04803	NM_001281454.2 Latest version!	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu21309	NM_001281453.2 Latest version!	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu04803	NM_001281454.1	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu21309	NM_001281453.1	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD3 ( NM_001281454.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268383.1 Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04803
Clone ID Related Accession (Same CDS sequence)	NM_001281454.1 , NM_001281454.2
Accession Version	NM_001281454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU181763.1, BC043619.1, BM684485.1 and AA576859.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.306394.1, SRR1163658.303003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA AGAGCCCGAG CGGGAAGAAG TTCCGCAGCA AGCCGCAGCT GGCGCGCTAC 
CTGGGCGGCT CCATGGACCT GAGCACCTTC GACTTCCGCA CGGGCAAGAT GCTGATGAGC 
AAGATGAACA AGAGCCGCCA GCGCGTGCGC TACGACTCCT CCAACCAGGT CAAGGGCAAG 
CCCGACCTGA ACACGGCGCT GCCCGTGCGC CAGACGGCGT CCATCTTCAA GCAGCCGGTG 
ACCAAGATTA CCAACCACCC CAGCAACAAG GTCAAGAGCG ACCCGCAGAA GGCGGTGGAC 
CAGCCGCGCC AGCTCTTCTG GGAGAAGAAG CTGAGCGGCC TGAACGCCTT CGACATTGCT 
GAGGAGCTGG TCAAGACCAT GGACCTCCCC AAGGGCCTGC AGGGGGTGGG ACCTGGCTGC 
ACGGATGAGA CGCTGCTGTC GGCCATCGCC AGCGCCCTGC ACACTAGCAC CATGCCCATC 
ACGGGACAGC TCTCGGCCGC CGTGGAGAAG AACCCCGGCG TATGGCTCAA CACCACGCAG 
CCCCTGTGCA AAGCCTTCAT GGTGACCGAC GAGGACATCA GGAAGCAGGA AGAGCTGGTG 
CAGCAGGTGC GGAAGCGGCT GGAGGAGGCG CTGATGGCCG ACATGCTGGC GCACGTGGAG 
GAGCTGGCCC GTGACGGGGA GGCGCCGCTG GACAAGGCCT GCGCTGAGGA CGACGACGAG 
GAAGACGAGG AGGAGGAGGA GGAGGAGCCC GACCCGGACC CGGAGATGGA GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268383.1
CDS	131..910
Translation	MERKSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

Target ORF information:

RefSeq Version	NM_001281454.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281454.1

ORF Insert Sequence:

ATGGAGCGGA AGAGCCCGAG CGGGAAGAAG TTCCGCAGCA AGCCGCAGCT GGCGCGCTAC 
CTGGGCGGCT CCATGGACCT GAGCACCTTC GACTTCCGCA CGGGCAAGAT GCTGATGAGC 
AAGATGAACA AGAGCCGCCA GCGCGTGCGC TACGACTCCT CCAACCAGGT CAAGGGCAAG 
CCCGACCTGA ACACGGCGCT GCCCGTGCGC CAGACGGCGT CCATCTTCAA GCAGCCGGTG 
ACCAAGATTA CCAACCACCC CAGCAACAAG GTCAAGAGCG ACCCGCAGAA GGCGGTGGAC 
CAGCCGCGCC AGCTCTTCTG GGAGAAGAAG CTGAGCGGCC TGAACGCCTT CGACATTGCT 
GAGGAGCTGG TCAAGACCAT GGACCTCCCC AAGGGCCTGC AGGGGGTGGG ACCTGGCTGC 
ACGGATGAGA CGCTGCTGTC GGCCATCGCC AGCGCCCTGC ACACTAGCAC CATGCCCATC 
ACGGGACAGC TCTCGGCCGC CGTGGAGAAG AACCCCGGCG TATGGCTCAA CACCACGCAG 
CCCCTGTGCA AAGCCTTCAT GGTGACCGAC GAGGACATCA GGAAGCAGGA AGAGCTGGTG 
CAGCAGGTGC GGAAGCGGCT GGAGGAGGCG CTGATGGCCG ACATGCTGGC GCACGTGGAG 
GAGCTGGCCC GTGACGGGGA GGCGCCGCTG GACAAGGCCT GCGCTGAGGA CGACGACGAG 
GAAGACGAGG AGGAGGAGGA GGAGGAGCCC GACCCGGACC CGGAGATGGA GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD3 ( NM_001281454.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268383.1 Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04803
Clone ID Related Accession (Same CDS sequence)	NM_001281454.1 , NM_001281454.2
Accession Version	NM_001281454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005943.1. On May 31, 2019 this sequence version replaced NM_001281454.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043619.1, SRR7410570.648284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA AGAGCCCGAG CGGGAAGAAG TTCCGCAGCA AGCCGCAGCT GGCGCGCTAC 
CTGGGCGGCT CCATGGACCT GAGCACCTTC GACTTCCGCA CGGGCAAGAT GCTGATGAGC 
AAGATGAACA AGAGCCGCCA GCGCGTGCGC TACGACTCCT CCAACCAGGT CAAGGGCAAG 
CCCGACCTGA ACACGGCGCT GCCCGTGCGC CAGACGGCGT CCATCTTCAA GCAGCCGGTG 
ACCAAGATTA CCAACCACCC CAGCAACAAG GTCAAGAGCG ACCCGCAGAA GGCGGTGGAC 
CAGCCGCGCC AGCTCTTCTG GGAGAAGAAG CTGAGCGGCC TGAACGCCTT CGACATTGCT 
GAGGAGCTGG TCAAGACCAT GGACCTCCCC AAGGGCCTGC AGGGGGTGGG ACCTGGCTGC 
ACGGATGAGA CGCTGCTGTC GGCCATCGCC AGCGCCCTGC ACACTAGCAC CATGCCCATC 
ACGGGACAGC TCTCGGCCGC CGTGGAGAAG AACCCCGGCG TATGGCTCAA CACCACGCAG 
CCCCTGTGCA AAGCCTTCAT GGTGACCGAC GAGGACATCA GGAAGCAGGA AGAGCTGGTG 
CAGCAGGTGC GGAAGCGGCT GGAGGAGGCG CTGATGGCCG ACATGCTGGC GCACGTGGAG 
GAGCTGGCCC GTGACGGGGA GGCGCCGCTG GACAAGGCCT GCGCTGAGGA CGACGACGAG 
GAAGACGAGG AGGAGGAGGA GGAGGAGCCC GACCCGGACC CGGAGATGGA GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268383.1
CDS	235..1014
Translation	MERKSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

Target ORF information:

RefSeq Version	NM_001281454.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281454.2

ORF Insert Sequence:

ATGGAGCGGA AGAGCCCGAG CGGGAAGAAG TTCCGCAGCA AGCCGCAGCT GGCGCGCTAC 
CTGGGCGGCT CCATGGACCT GAGCACCTTC GACTTCCGCA CGGGCAAGAT GCTGATGAGC 
AAGATGAACA AGAGCCGCCA GCGCGTGCGC TACGACTCCT CCAACCAGGT CAAGGGCAAG 
CCCGACCTGA ACACGGCGCT GCCCGTGCGC CAGACGGCGT CCATCTTCAA GCAGCCGGTG 
ACCAAGATTA CCAACCACCC CAGCAACAAG GTCAAGAGCG ACCCGCAGAA GGCGGTGGAC 
CAGCCGCGCC AGCTCTTCTG GGAGAAGAAG CTGAGCGGCC TGAACGCCTT CGACATTGCT 
GAGGAGCTGG TCAAGACCAT GGACCTCCCC AAGGGCCTGC AGGGGGTGGG ACCTGGCTGC 
ACGGATGAGA CGCTGCTGTC GGCCATCGCC AGCGCCCTGC ACACTAGCAC CATGCCCATC 
ACGGGACAGC TCTCGGCCGC CGTGGAGAAG AACCCCGGCG TATGGCTCAA CACCACGCAG 
CCCCTGTGCA AAGCCTTCAT GGTGACCGAC GAGGACATCA GGAAGCAGGA AGAGCTGGTG 
CAGCAGGTGC GGAAGCGGCT GGAGGAGGCG CTGATGGCCG ACATGCTGGC GCACGTGGAG 
GAGCTGGCCC GTGACGGGGA GGCGCCGCTG GACAAGGCCT GCGCTGAGGA CGACGACGAG 
GAAGACGAGG AGGAGGAGGA GGAGGAGCCC GACCCGGACC CGGAGATGGA GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD3 ( NM_001281453.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268382.1 Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21309
Clone ID Related Accession (Same CDS sequence)	NM_001281453.1 , NM_001281453.2
Accession Version	NM_001281453.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU181763.1, AK290882.1, BC043619.1, BM684485.1 and AA576859.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072247.1, AK290882.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA AGAGGTGGGA GTGCCCGGCG CTCCCGCAGG GCTGGGAGAG GGAAGAAGTG 
CCCAGAAGGT CGGGGCTGTC GGCCGGCCAC AGGGATGTCT TTTACTATAG CCCGAGCGGG 
AAGAAGTTCC GCAGCAAGCC GCAGCTGGCG CGCTACCTGG GCGGCTCCAT GGACCTGAGC 
ACCTTCGACT TCCGCACGGG CAAGATGCTG ATGAGCAAGA TGAACAAGAG CCGCCAGCGC 
GTGCGCTACG ACTCCTCCAA CCAGGTCAAG GGCAAGCCCG ACCTGAACAC GGCGCTGCCC 
GTGCGCCAGA CGGCGTCCAT CTTCAAGCAG CCGGTGACCA AGATTACCAA CCACCCCAGC 
AACAAGGTCA AGAGCGACCC GCAGAAGGCG GTGGACCAGC CGCGCCAGCT CTTCTGGGAG 
AAGAAGCTGA GCGGCCTGAA CGCCTTCGAC ATTGCTGAGG AGCTGGTCAA GACCATGGAC 
CTCCCCAAGG GCCTGCAGGG GGTGGGACCT GGCTGCACGG ATGAGACGCT GCTGTCGGCC 
ATCGCCAGCG CCCTGCACAC TAGCACCATG CCCATCACGG GACAGCTCTC GGCCGCCGTG 
GAGAAGAACC CCGGCGTATG GCTCAACACC ACGCAGCCCC TGTGCAAAGC CTTCATGGTG 
ACCGACGAGG ACATCAGGAA GCAGGAAGAG CTGGTGCAGC AGGTGCGGAA GCGGCTGGAG 
GAGGCGCTGA TGGCCGACAT GCTGGCGCAC GTGGAGGAGC TGGCCCGTGA CGGGGAGGCG 
CCGCTGGACA AGGCCTGCGC TGAGGACGAC GACGAGGAAG ACGAGGAGGA GGAGGAGGAG 
GAGCCCGACC CGGACCCGGA GATGGAGCAC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268382.1
CDS	131..1006
Translation	MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

Target ORF information:

RefSeq Version	NM_001281453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281453.1

ORF Insert Sequence:

ATGGAGCGGA AGAGGTGGGA GTGCCCGGCG CTCCCGCAGG GCTGGGAGAG GGAAGAAGTG 
CCCAGAAGGT CGGGGCTGTC GGCCGGCCAC AGGGATGTCT TTTACTATAG CCCGAGCGGG 
AAGAAGTTCC GCAGCAAGCC GCAGCTGGCG CGCTACCTGG GCGGCTCCAT GGACCTGAGC 
ACCTTCGACT TCCGCACGGG CAAGATGCTG ATGAGCAAGA TGAACAAGAG CCGCCAGCGC 
GTGCGCTACG ACTCCTCCAA CCAGGTCAAG GGCAAGCCCG ACCTGAACAC GGCGCTGCCC 
GTGCGCCAGA CGGCGTCCAT CTTCAAGCAG CCGGTGACCA AGATTACCAA CCACCCCAGC 
AACAAGGTCA AGAGCGACCC GCAGAAGGCG GTGGACCAGC CGCGCCAGCT CTTCTGGGAG 
AAGAAGCTGA GCGGCCTGAA CGCCTTCGAC ATTGCTGAGG AGCTGGTCAA GACCATGGAC 
CTCCCCAAGG GCCTGCAGGG GGTGGGACCT GGCTGCACGG ATGAGACGCT GCTGTCGGCC 
ATCGCCAGCG CCCTGCACAC TAGCACCATG CCCATCACGG GACAGCTCTC GGCCGCCGTG 
GAGAAGAACC CCGGCGTATG GCTCAACACC ACGCAGCCCC TGTGCAAAGC CTTCATGGTG 
ACCGACGAGG ACATCAGGAA GCAGGAAGAG CTGGTGCAGC AGGTGCGGAA GCGGCTGGAG 
GAGGCGCTGA TGGCCGACAT GCTGGCGCAC GTGGAGGAGC TGGCCCGTGA CGGGGAGGCG 
CCGCTGGACA AGGCCTGCGC TGAGGACGAC GACGAGGAAG ACGAGGAGGA GGAGGAGGAG 
GAGCCCGACC CGGACCCGGA GATGGAGCAC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD3 ( NM_001281453.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268382.1 Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21309
Clone ID Related Accession (Same CDS sequence)	NM_001281453.1 , NM_001281453.2
Accession Version	NM_001281453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005943.1. On Nov 22, 2018 this sequence version replaced NM_001281453.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072247.1, AK290882.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000434436.8/ ENSP00000412302.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCGGA AGAGGTGGGA GTGCCCGGCG CTCCCGCAGG GCTGGGAGAG GGAAGAAGTG 
CCCAGAAGGT CGGGGCTGTC GGCCGGCCAC AGGGATGTCT TTTACTATAG CCCGAGCGGG 
AAGAAGTTCC GCAGCAAGCC GCAGCTGGCG CGCTACCTGG GCGGCTCCAT GGACCTGAGC 
ACCTTCGACT TCCGCACGGG CAAGATGCTG ATGAGCAAGA TGAACAAGAG CCGCCAGCGC 
GTGCGCTACG ACTCCTCCAA CCAGGTCAAG GGCAAGCCCG ACCTGAACAC GGCGCTGCCC 
GTGCGCCAGA CGGCGTCCAT CTTCAAGCAG CCGGTGACCA AGATTACCAA CCACCCCAGC 
AACAAGGTCA AGAGCGACCC GCAGAAGGCG GTGGACCAGC CGCGCCAGCT CTTCTGGGAG 
AAGAAGCTGA GCGGCCTGAA CGCCTTCGAC ATTGCTGAGG AGCTGGTCAA GACCATGGAC 
CTCCCCAAGG GCCTGCAGGG GGTGGGACCT GGCTGCACGG ATGAGACGCT GCTGTCGGCC 
ATCGCCAGCG CCCTGCACAC TAGCACCATG CCCATCACGG GACAGCTCTC GGCCGCCGTG 
GAGAAGAACC CCGGCGTATG GCTCAACACC ACGCAGCCCC TGTGCAAAGC CTTCATGGTG 
ACCGACGAGG ACATCAGGAA GCAGGAAGAG CTGGTGCAGC AGGTGCGGAA GCGGCTGGAG 
GAGGCGCTGA TGGCCGACAT GCTGGCGCAC GTGGAGGAGC TGGCCCGTGA CGGGGAGGCG 
CCGCTGGACA AGGCCTGCGC TGAGGACGAC GACGAGGAAG ACGAGGAGGA GGAGGAGGAG 
GAGCCCGACC CGGACCCGGA GATGGAGCAC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268382.1
CDS	235..1110
Translation	MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

Target ORF information:

RefSeq Version	NM_001281453.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 3 (MBD3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281453.2

ORF Insert Sequence:

ATGGAGCGGA AGAGGTGGGA GTGCCCGGCG CTCCCGCAGG GCTGGGAGAG GGAAGAAGTG 
CCCAGAAGGT CGGGGCTGTC GGCCGGCCAC AGGGATGTCT TTTACTATAG CCCGAGCGGG 
AAGAAGTTCC GCAGCAAGCC GCAGCTGGCG CGCTACCTGG GCGGCTCCAT GGACCTGAGC 
ACCTTCGACT TCCGCACGGG CAAGATGCTG ATGAGCAAGA TGAACAAGAG CCGCCAGCGC 
GTGCGCTACG ACTCCTCCAA CCAGGTCAAG GGCAAGCCCG ACCTGAACAC GGCGCTGCCC 
GTGCGCCAGA CGGCGTCCAT CTTCAAGCAG CCGGTGACCA AGATTACCAA CCACCCCAGC 
AACAAGGTCA AGAGCGACCC GCAGAAGGCG GTGGACCAGC CGCGCCAGCT CTTCTGGGAG 
AAGAAGCTGA GCGGCCTGAA CGCCTTCGAC ATTGCTGAGG AGCTGGTCAA GACCATGGAC 
CTCCCCAAGG GCCTGCAGGG GGTGGGACCT GGCTGCACGG ATGAGACGCT GCTGTCGGCC 
ATCGCCAGCG CCCTGCACAC TAGCACCATG CCCATCACGG GACAGCTCTC GGCCGCCGTG 
GAGAAGAACC CCGGCGTATG GCTCAACACC ACGCAGCCCC TGTGCAAAGC CTTCATGGTG 
ACCGACGAGG ACATCAGGAA GCAGGAAGAG CTGGTGCAGC AGGTGCGGAA GCGGCTGGAG 
GAGGCGCTGA TGGCCGACAT GCTGGCGCAC GTGGAGGAGC TGGCCCGTGA CGGGGAGGCG 
CCGCTGGACA AGGCCTGCGC TGAGGACGAC GACGAGGAAG ACGAGGAGGA GGAGGAGGAG 
GAGCCCGACC CGGACCCGGA GATGGAGCAC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-?/Smad signalling.
British journal of cancer116(1)91-99(2017 Jan)
Xu M,He J,Li J,Feng W,Zhou H,Wei H,Zhou M,Lu Y,Zeng J,Peng W,Du F,Gong A

MBD3 inhibits formation of liver cancer stem cells.
Oncotarget8(4)6067-6078(2017 Jan)
Li R,He Q,Han S,Zhang M,Liu J,Su M,Wei S,Wang X,Shen L

Regulatory landscape and clinical implication of MBD3 in human malignant glioma.
Oncotarget7(49)81698-81714(2016 Dec)
Cui Y,Li J,Weng L,Wirbisky SE,Freeman JL,Liu J,Liu Q,Yuan X,Irudayaraj J

MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13455-13467(2016 Oct)
Cui J,Duan B,Zhao X,Chen Y,Sun S,Deng W,Zhang Y,Du J,Chen Y,Gu L

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MBD3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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