PLOD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLOD1
Entrez Gene ID	5351
Full Name	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Synonyms	EDS6,EDSKCL1,LH,LH1,LLH,PLOD
General protein information	Preferred Names procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Names procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 lysine hydroxylase lysyl hydroxlase 1 procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.22
Summary	Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	153454
Disorder Html:	Ehlers-Danlos syndrome, type VI, 225400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024447707.1	XP_024303475.1	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X1
NM_001316320.2	NP_001303249.1	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 1 precursor
NM_000302.3	NP_000293.2	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 2 precursor
NM_000302.4	NP_000293.2	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 2 precursor
NM_001316320.1	NP_001303249.1	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLOD1	XP_001142788.1
Canis lupus familiaris (dog)	PLOD1	XP_544565.2
Danio rerio (zebrafish)	plod1a	NP_001071210.2
Bos taurus (cattle)	PLOD1	NP_776573.1
Rattus norvegicus (Norway rat)	Plod1	NP_446279.2
Xenopus tropicalis (tropical clawed frog)	plod1	NP_001072340.1
Homo sapiens (human)	PLOD1	NP_000293.2
Mus musculus (house mouse)	Plod1	NP_035252.1
Gallus gallus (chicken)	PLOD1	NP_001005618.1

Gene Ontology
Bibliography

Related articles in PubMed

Ehlers Danlos syndrome, kyphoscoliotic type due to Lysyl Hydroxylase 1 deficiency in two children without congenital or early onset kyphoscoliosis.
van Dijk FS, Mancini GMS, Maugeri A, Cobben JM
European journal of medical genetics60(10)536-540(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Kyphoscoliotic type of Ehlers-Danlos Syndrome (EDS VIA) in six Egyptian patients presenting with a homogeneous clinical phenotype.
Abdalla EM, Rohrbach M, B?rer C, Kraenzlin M, El-Tayeby H, Elbelbesy MF, Nabil A, Giunta C
European journal of pediatrics174(1)105-12(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ehlers Danlos syndrome in two children was confirmed by mutations in PLOD1 gene. Initially patients were thought to have either a diagnosis of classical EDS (cEDS) or a neuromuscular disorder due to absence of (congenital) scoliosis.
Title: Ehlers Danlos syndrome, kyphoscoliotic type due to Lysyl Hydroxylase 1 deficiency in two children without congenital or early onset kyphoscoliosis.

Deficiency of the enzyme lysyl hydroxylase 1 (LH1) due to mutations in PLOD1 results in underhydroxylation of collagen lysyl residues and, hence, in the abnormal formation of collagen cross-links.
Title: Kyphoscoliotic type of Ehlers-Danlos Syndrome (EDS VIA) in six Egyptian patients presenting with a homogeneous clinical phenotype.

Compared to slow progressing Alzheimer's disease, PLOD1 is upregulated in fast progressing Alzheimer's disease ;patients.
Title: Gene expression profiling of peripheral blood leukocytes shows consistent longitudinal downregulation of TOMM40 and upregulation of KIR2DL5A, PLOD1, and SLC2A8 among fast progressors in early Alzheimer's disease.

Data show that homozygous and compound heterozygous changes found in PLOD1 and SLC2A10 may confer autosomal recessive effects, and three MYH11, ACTA2 and COL3A1 heterozygous variants were considered as putative pathogenic gene alterations.
Title: Rapid detection of gene mutations responsible for non-syndromic aortic aneurysm and dissection using two different methods: resequencing microarray technology and next-generation sequencing.

Common polymorphisms in four candidate genes (COL11A1, COL18A1, FBN1 and PLOD1) were unlikely to play important roles in the genetic susceptibility to high myopia.
Title: A DNA pooling-based case-control study of myopia candidate genes COL11A1, COL18A1, FBN1, and PLOD1 in a Chinese population.

The following PLOD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLOD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18408	NM_000302.4 Latest version!	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu59781	NM_001316320.2 Latest version!	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu105076	XM_024447707.1 Latest version!	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu18408	NM_000302.3	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu59781	NM_001316320.1	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLOD1 ( NM_000302.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000293.2 Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18408
Clone ID Related Accession (Same CDS sequence)	NM_000302.3 , NM_000302.4
Accession Version	NM_000302.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334246.1, M98252.1 and BQ447373.1. This sequence is a reference standard in the RefSeqGene project. On Mar 1, 2011 this sequence version replaced NM_000302.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.553877.1, SRR1803611.179944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA 
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA 
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG 
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC 
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC 
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC 
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT 
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG 
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG 
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG 
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC 
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC 
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT 
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT 
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT 
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT 
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT 
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC 
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC 
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG 
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG 
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT 
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC 
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC 
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC 
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC 
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC 
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT 
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC 
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT 
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC 
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC 
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT 
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC 
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC 
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000293.2
CDS	114..2297
Translation	MRPLLLLALLGWLLLAEAKGDAKPEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_000302.3
Organism	Homo sapiens(human)
Definition	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000302.3

ORF Insert Sequence:

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA 
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA 
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG 
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC 
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC 
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC 
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT 
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG 
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG 
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG 
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC 
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC 
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT 
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT 
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT 
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT 
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT 
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC 
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC 
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG 
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG 
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT 
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC 
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC 
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC 
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC 
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC 
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT 
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC 
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT 
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC 
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC 
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT 
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC 
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC 
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLOD1 ( NM_000302.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000293.2 Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18408
Clone ID Related Accession (Same CDS sequence)	NM_000302.3 , NM_000302.4
Accession Version	NM_000302.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334246.1, M98252.1 and BQ447373.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000302.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.277372.1, SRR1803611.179944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000196061.5/ ENSP00000196061.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA 
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA 
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG 
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC 
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC 
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC 
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT 
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG 
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG 
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG 
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC 
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC 
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT 
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT 
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT 
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT 
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT 
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC 
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC 
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG 
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG 
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT 
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC 
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC 
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC 
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC 
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC 
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT 
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC 
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT 
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC 
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC 
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT 
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC 
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC 
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000293.2
CDS	64..2247
Translation	MRPLLLLALLGWLLLAEAKGDAKPEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_000302.4
Organism	Homo sapiens(human)
Definition	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000302.4

ORF Insert Sequence:

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA 
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA 
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG 
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC 
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC 
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC 
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT 
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG 
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG 
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG 
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC 
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC 
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT 
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT 
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT 
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT 
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT 
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC 
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC 
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG 
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG 
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT 
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC 
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC 
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC 
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC 
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC 
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT 
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC 
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT 
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC 
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC 
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT 
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC 
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC 
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLOD1 ( NM_001316320.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303249.1 Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu59781
Clone ID Related Accession (Same CDS sequence)	NM_001316320.2 , NM_001316320.1
Accession Version	NM_001316320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334246.1, AK299150.1, AI347587.1 and BM991136.1. On May 31, 2019 this sequence version replaced NM_001316320.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.7413.1, AK316285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT 
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG 
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC 
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT 
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT 
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC 
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC 
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC 
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC 
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG 
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC 
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT 
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG 
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC 
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC 
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC 
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG 
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG 
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC 
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG 
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG 
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC 
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC 
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC 
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC 
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC 
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG 
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG 
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG 
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC 
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC 
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC 
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG 
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG 
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG 
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC 
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303249.1
CDS	64..2388
Translation	MRPLLLLALLGWLLLAEAKGDAKPEAPCCQEGLRAGGSGSLHLGRDFTVLAGARGSPSPSVSSIPRFWIPGSDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_001316320.2
Organism	Homo sapiens(human)
Definition	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316320.2

ORF Insert Sequence:

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT 
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG 
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC 
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT 
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT 
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC 
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC 
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC 
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC 
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG 
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC 
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT 
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG 
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC 
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC 
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC 
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG 
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG 
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC 
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG 
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG 
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC 
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC 
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC 
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC 
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC 
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG 
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG 
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG 
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC 
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC 
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC 
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG 
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG 
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG 
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC 
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLOD1 ( NM_001316320.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303249.1 Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu59781
Clone ID Related Accession (Same CDS sequence)	NM_001316320.2 , NM_001316320.1
Accession Version	NM_001316320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334246.1, AK299150.1, AI347587.1 and BM991136.1. On Oct 14, 2015 this sequence version replaced XM_011541593.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316285.1, AK299150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT 
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG 
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC 
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT 
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT 
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC 
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC 
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC 
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC 
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG 
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC 
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT 
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG 
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC 
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC 
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC 
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG 
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG 
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC 
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG 
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG 
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC 
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC 
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC 
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC 
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC 
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG 
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG 
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG 
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC 
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC 
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC 
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG 
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG 
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG 
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC 
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303249.1
CDS	114..2438
Translation	MRPLLLLALLGWLLLAEAKGDAKPEAPCCQEGLRAGGSGSLHLGRDFTVLAGARGSPSPSVSSIPRFWIPGSDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_001316320.1
Organism	Homo sapiens(human)
Definition	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316320.1

ORF Insert Sequence:

ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT 
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG 
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC 
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT 
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT 
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC 
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC 
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC 
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC 
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG 
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC 
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT 
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG 
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC 
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC 
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC 
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG 
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG 
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC 
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG 
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG 
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC 
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC 
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC 
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC 
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC 
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG 
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG 
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG 
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC 
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC 
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC 
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG 
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG 
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG 
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC 
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLOD1 ( XM_024447707.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303475.1 Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X1, mRNA.

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CloneID	OHu105076
Clone ID Related Accession (Same CDS sequence)	XM_024447707.1
Accession Version	XM_024447707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCATG TGAGAGCGAG GAACCTGGCC TATGACACCC TCCCGGTCCT GATCCATGGC 
AACGGGCCAA CCAAGCTGCA GTTGAACTAC CTGGGCAACT ACATCCCGCG CTTCTGGACC 
TTCGAAACAG GCTGCACCGT GTGTGACGAA GGCTTGCGCA GCCTCAAGGG CATTGGGGAT 
GAAGCTCTGC CCACGGTCCT GGTCGGCGTG TTCATCGAAC AGCCCACGCC GTTTGTGTCC 
CTGTTCTTCC AGCGGCTCCT GCGGCTCCAC TACCCCCAGA AACACATGCG ACTTTTCATC 
CACAACCACG AGCAGCACCA CAAGGCTCAG GTGGAAGAGT TCCTGGCACA GCATGGCAGC 
GAGTACCAGT CTGTGAAGCT GGTGGGCCCT GAGGTGCGGA TGGCGAATGC AGATGCCAGG 
AACATGGGCG CAGACCTGTG CCGGCAGGAC CGCAGCTGCA CCTACTACTT CAGCGTGGAT 
GCTGACGTGG CCCTGACCGA GCCCAACAGC CTGCGGCTGC TGATCCAACA GAACAAGAAC 
GTCATTGCCC CGCTGATGAC CCGGCATGGG AGGCTGTGGT CGAACTTCTG GGGGGCTCTC 
AGTGCAGATG GCTACTATGC CCGTTCCGAG GACTACGTGG ACATTGTGCA GGGGCGGCGT 
GTTGGTGTCT GGAATGTGCC CTATATTTCA AACATCTACT TGATCAAGGG CAGTGCCCTG 
CGGGGTGAGC TGCAGTCCTC AGATCTCTTC CACCACAGCA AGCTGGACCC CGACATGGCC 
TTCTGTGCCA ACATCCGGCA GCAGGATGTG TTCATGTTCC TGACCAACCG GCACACCCTT 
GGCCATCTGC TCTCCCTAGA CAGCTACCGC ACCACCCACC TGCACAACGA CCTCTGGGAG 
GTGTTCAGCA ACCCCGAGGA CTGGAAGGAG AAGTACATCC ACCAGAACTA CACCAAAGCC 
CTGGCAGGGA AGCTGGTGGA GACGCCCTGC CCGGATGTCT ATTGGTTCCC CATCTTCACG 
GAGGTGGCCT GTGATGAGCT GGTGGAGGAG ATGGAGCACT TTGGCCAGTG GTCTCTGGGC 
AACAACAAGG ACAACCGCAT CCAGGGTGGC TACGAGAACG TGCCGACTAT TGACATCCAC 
ATGAACCAGA TCGGCTTTGA GCGGGAGTGG CACAAATTCC TGCTGGAGTA CATTGCGCCC 
ATGACGGAGA AGCTCTACCC CGGCTACTAC ACCAGGGCCC AGTTTGACCT GGCCTTTGTC 
GTCCGCTACA AGCCTGATGA GCAGCCCTCA CTGATGCCAC ACCATGATGC CTCCACCTTC 
ACCATCAACA TCGCCCTGAA CCGAGTCGGG GTGGATTACG AGGGCGGGGG CTGTCGGTTC 
CTGCGCTACA ACTGTTCCAT CCGAGCCCCA AGGAAGGGCT GGACCCTCAT GCACCCTGGA 
CGACTCACGC ATTACCATGA GGGGCTCCCC ACCACCAGGG GCACCCGCTA CATCGCAGTC 
TCCTTCGTCG ATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303475.1
CDS	761..2278
Translation	MGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP

Target ORF information:

RefSeq Version	XM_024447707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447707.1

ORF Insert Sequence:

ATGGGCCATG TGAGAGCGAG GAACCTGGCC TATGACACCC TCCCGGTCCT GATCCATGGC 
AACGGGCCAA CCAAGCTGCA GTTGAACTAC CTGGGCAACT ACATCCCGCG CTTCTGGACC 
TTCGAAACAG GCTGCACCGT GTGTGACGAA GGCTTGCGCA GCCTCAAGGG CATTGGGGAT 
GAAGCTCTGC CCACGGTCCT GGTCGGCGTG TTCATCGAAC AGCCCACGCC GTTTGTGTCC 
CTGTTCTTCC AGCGGCTCCT GCGGCTCCAC TACCCCCAGA AACACATGCG ACTTTTCATC 
CACAACCACG AGCAGCACCA CAAGGCTCAG GTGGAAGAGT TCCTGGCACA GCATGGCAGC 
GAGTACCAGT CTGTGAAGCT GGTGGGCCCT GAGGTGCGGA TGGCGAATGC AGATGCCAGG 
AACATGGGCG CAGACCTGTG CCGGCAGGAC CGCAGCTGCA CCTACTACTT CAGCGTGGAT 
GCTGACGTGG CCCTGACCGA GCCCAACAGC CTGCGGCTGC TGATCCAACA GAACAAGAAC 
GTCATTGCCC CGCTGATGAC CCGGCATGGG AGGCTGTGGT CGAACTTCTG GGGGGCTCTC 
AGTGCAGATG GCTACTATGC CCGTTCCGAG GACTACGTGG ACATTGTGCA GGGGCGGCGT 
GTTGGTGTCT GGAATGTGCC CTATATTTCA AACATCTACT TGATCAAGGG CAGTGCCCTG 
CGGGGTGAGC TGCAGTCCTC AGATCTCTTC CACCACAGCA AGCTGGACCC CGACATGGCC 
TTCTGTGCCA ACATCCGGCA GCAGGATGTG TTCATGTTCC TGACCAACCG GCACACCCTT 
GGCCATCTGC TCTCCCTAGA CAGCTACCGC ACCACCCACC TGCACAACGA CCTCTGGGAG 
GTGTTCAGCA ACCCCGAGGA CTGGAAGGAG AAGTACATCC ACCAGAACTA CACCAAAGCC 
CTGGCAGGGA AGCTGGTGGA GACGCCCTGC CCGGATGTCT ATTGGTTCCC CATCTTCACG 
GAGGTGGCCT GTGATGAGCT GGTGGAGGAG ATGGAGCACT TTGGCCAGTG GTCTCTGGGC 
AACAACAAGG ACAACCGCAT CCAGGGTGGC TACGAGAACG TGCCGACTAT TGACATCCAC 
ATGAACCAGA TCGGCTTTGA GCGGGAGTGG CACAAATTCC TGCTGGAGTA CATTGCGCCC 
ATGACGGAGA AGCTCTACCC CGGCTACTAC ACCAGGGCCC AGTTTGACCT GGCCTTTGTC 
GTCCGCTACA AGCCTGATGA GCAGCCCTCA CTGATGCCAC ACCATGATGC CTCCACCTTC 
ACCATCAACA TCGCCCTGAA CCGAGTCGGG GTGGATTACG AGGGCGGGGG CTGTCGGTTC 
CTGCGCTACA ACTGTTCCAT CCGAGCCCCA AGGAAGGGCT GGACCCTCAT GCACCCTGGA 
CGACTCACGC ATTACCATGA GGGGCTCCCC ACCACCAGGG GCACCCGCTA CATCGCAGTC 
TCCTTCGTCG ATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ehlers Danlos syndrome, kyphoscoliotic type due to Lysyl Hydroxylase 1 deficiency in two children without congenital or early onset kyphoscoliosis.
European journal of medical genetics60(10)536-540(2017 Oct)
van Dijk FS,Mancini GMS,Maugeri A,Cobben JM

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Kyphoscoliotic type of Ehlers-Danlos Syndrome (EDS VIA) in six Egyptian patients presenting with a homogeneous clinical phenotype.
European journal of pediatrics174(1)105-12(2015 Jan)
Abdalla EM,Rohrbach M,B?rer C,Kraenzlin M,El-Tayeby H,Elbelbesy MF,Nabil A,Giunta C

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PLOD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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