PLD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLD2
Entrez Gene ID	5338
Full Name	phospholipase D2
Synonyms	PLD1C
General protein information	Preferred Names phospholipase D2 Names phospholipase D2 choline phosphatase 2 phosphatidylcholine-hydrolyzing phospholipase D2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Summary	The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011].
Disorder MIM:	602384

mRNA and Protein(s)

mRNA	Protein	Name
NM_001243108.1	NP_001230037.1	phospholipase D2 isoform PLD2B
XM_005256696.2	XP_005256753.1	phospholipase D2 isoform X1
NM_001243108.2	NP_001230037.1	phospholipase D2 isoform PLD2B
NM_002663.5	NP_002654.3	phospholipase D2 isoform PLD2A
XM_017024764.2	XP_016880253.1	phospholipase D2 isoform X2
XM_017024764.1	XP_016880253.1	phospholipase D2 isoform X2
NM_002663.4	NP_002654.3	phospholipase D2 isoform PLD2A

Pathways from BioSystems

Homologies

	PLD2	XP_001117754.2
Canis lupus familiaris (dog)	PLD2	XP_005620373.1
Pan troglodytes (chimpanzee)	PLD2	XP_511291.3
Homo sapiens (human)	PLD2	NP_002654.3
Mus musculus (house mouse)	Pld2	NP_032902.1
Rattus norvegicus (Norway rat)	Pld2	NP_150641.2
Danio rerio (zebrafish)	pld2	XP_694649.4
Bos taurus (cattle)	PLD2	NP_001069295.1
Xenopus tropicalis (tropical clawed frog)	pld2	NP_001135641.1

Gene Ontology
Bibliography

Related articles in PubMed

Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway.
Nelson RK, Ya-Ping J, Gadbery J, Abedeen D, Sampson N, Lin RZ, Frohman MA
Scientific reports7(1)9112(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest PLD expression in high grade serous ovarian carcinoma may have a role in mediating progression to effusions and chemoresistance.
Title: Phospholipase D messenger RNA expression and clinical role in high-grade serous carcinoma.

results suggest that the small GTPase RalA plays an important role in promoting invagination and trafficking of caveolae, not by potentiating the association between Cav-1 and FilA but by stimulating PLD2-mediated generation of phosphatidic acid.
Title: Phosphatidic Acid Produced by RalA-activated PLD2 Stimulates Caveolae-mediated Endocytosis and Trafficking in Endothelial Cells.

Data suggest that elevated membrane tension acts through phospholipase D2 (PLD2) and the mammalian target of rapamycin complex 2 (mTORC2) to limit actin nucleation.
Title: Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration.

Slug is a positive regulator, and Snail a negative regulator, of PLD2 expression.
Title: The transcription factors Slug (SNAI2) and Snail (SNAI1) regulate phospholipase D (PLD) promoter in opposite ways towards cancer cell invasion.

PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells.
Title: Loss of phospholipase D2 impairs VEGF-induced angiogenesis.

The following PLD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu49880	XM_005256696.2 Latest version!	Homo sapiens phospholipase D2 (PLD2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu15841	NM_001243108.2 Latest version!	Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu19303	NM_002663.5 Latest version!	Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu86927	XM_017024764.2 Latest version!	Homo sapiens phospholipase D2 (PLD2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu15841	NM_001243108.1	Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu86927	XM_017024764.1	Homo sapiens phospholipase D2 (PLD2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu19303	NM_002663.4	Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLD2 ( XM_005256696.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256753.1 Homo sapiens phospholipase D2 (PLD2), transcript variant X6, mRNA.

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CloneID	OHu49880
Clone ID Related Accession (Same CDS sequence)	XM_005256696.2
Accession Version	XM_005256696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005256696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGGTTTGC CTTGAGTCTG CGGAAGCACT GCTTCGGTGT GATTCTTGGA 
GCAAATACCC GGCCAGACTT GGATCTCCGA GACCCCATCT GTGATGACTT CTTCCAGTTG 
TGGCAAGACA TGGCTGAGAG CAACGCCAAT ATCTATGAGC AGATCTTCCG CTGCCTGCCA 
TCCAATGCCA CGCGTTCCCT GCGGACTCTC CGGGAGTACG TGGCCGTGGA GCCCTTGGCC 
ACGGTCAGTC CCCCCTTGGC TCGGTCTGAG CTCACCCAGG TCCAGGGCCA CCTGGTCCAC 
TTCCCCCTCA AGTTCCTAGA GGATGAGTCT TTGCTGCCCC CGCTGGGTAG CAAGGAGGGC 
ATGATCCCCC TAGAAGTGTG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256753.1
CDS	342..1988
Translation	MLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	XM_005256696.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256696.2

ORF Insert Sequence:

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGGTTTGC CTTGAGTCTG CGGAAGCACT GCTTCGGTGT GATTCTTGGA 
GCAAATACCC GGCCAGACTT GGATCTCCGA GACCCCATCT GTGATGACTT CTTCCAGTTG 
TGGCAAGACA TGGCTGAGAG CAACGCCAAT ATCTATGAGC AGATCTTCCG CTGCCTGCCA 
TCCAATGCCA CGCGTTCCCT GCGGACTCTC CGGGAGTACG TGGCCGTGGA GCCCTTGGCC 
ACGGTCAGTC CCCCCTTGGC TCGGTCTGAG CTCACCCAGG TCCAGGGCCA CCTGGTCCAC 
TTCCCCCTCA AGTTCCTAGA GGATGAGTCT TTGCTGCCCC CGCTGGGTAG CAAGGAGGGC 
ATGATCCCCC TAGAAGTGTG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( NM_001243108.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230037.1 Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15841
Clone ID Related Accession (Same CDS sequence)	NM_001243108.1 , NM_001243108.2
Accession Version	NM_001243108.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform PLD2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA390950.1, DC407587.1, AF033850.2, AF038440.1, AF038441.1 and BC015033.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (PLD2B) has the same N- and C-termini but is shorter compared to isoform PLD2A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.102878.1, AF038441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGT GTGATTCTTG GAGCAAATAC CCGGCCAGAC 
TTGGATCTCC GAGACCCCAT CTGTGATGAC TTCTTCCAGT TGTGGCAAGA CATGGCTGAG 
AGCAACGCCA ATATCTATGA GCAGATCTTC CGCTGCCTGC CATCCAATGC CACGCGTTCC 
CTGCGGACTC TCCGGGAGTA CGTGGCCGTG GAGCCCTTGG CCACGGTCAG TCCCCCCTTG 
GCTCGGTCTG AGCTCACCCA GGTCCAGGGC CACCTGGTCC ACTTCCCCCT CAAGTTCCTA 
GAGGATGAGT CTTTGCTGCC CCCGCTGGGT AGCAAGGAGG GCATGATCCC CCTAGAAGTG 
TGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230037.1
CDS	127..2895
Translation	MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGSVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	NM_001243108.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243108.1

ORF Insert Sequence:

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGT GTGATTCTTG GAGCAAATAC CCGGCCAGAC 
TTGGATCTCC GAGACCCCAT CTGTGATGAC TTCTTCCAGT TGTGGCAAGA CATGGCTGAG 
AGCAACGCCA ATATCTATGA GCAGATCTTC CGCTGCCTGC CATCCAATGC CACGCGTTCC 
CTGCGGACTC TCCGGGAGTA CGTGGCCGTG GAGCCCTTGG CCACGGTCAG TCCCCCCTTG 
GCTCGGTCTG AGCTCACCCA GGTCCAGGGC CACCTGGTCC ACTTCCCCCT CAAGTTCCTA 
GAGGATGAGT CTTTGCTGCC CCCGCTGGGT AGCAAGGAGG GCATGATCCC CCTAGAAGTG 
TGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( NM_001243108.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230037.1 Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.

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CloneID	OHu15841
Clone ID Related Accession (Same CDS sequence)	NM_001243108.1 , NM_001243108.2
Accession Version	NM_001243108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform PLD2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407587.1, AF033850.2, AF038440.1, AF038441.1 and BC015033.1. On May 31, 2019 this sequence version replaced NM_001243108.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (PLD2B) has the same N- and C-termini but is shorter compared to isoform PLD2A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.86022.1, SRR1660807.102878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGT GTGATTCTTG GAGCAAATAC CCGGCCAGAC 
TTGGATCTCC GAGACCCCAT CTGTGATGAC TTCTTCCAGT TGTGGCAAGA CATGGCTGAG 
AGCAACGCCA ATATCTATGA GCAGATCTTC CGCTGCCTGC CATCCAATGC CACGCGTTCC 
CTGCGGACTC TCCGGGAGTA CGTGGCCGTG GAGCCCTTGG CCACGGTCAG TCCCCCCTTG 
GCTCGGTCTG AGCTCACCCA GGTCCAGGGC CACCTGGTCC ACTTCCCCCT CAAGTTCCTA 
GAGGATGAGT CTTTGCTGCC CCCGCTGGGT AGCAAGGAGG GCATGATCCC CCTAGAAGTG 
TGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230037.1
CDS	76..2844
Translation	MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGSVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	NM_001243108.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243108.2

ORF Insert Sequence:

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGT GTGATTCTTG GAGCAAATAC CCGGCCAGAC 
TTGGATCTCC GAGACCCCAT CTGTGATGAC TTCTTCCAGT TGTGGCAAGA CATGGCTGAG 
AGCAACGCCA ATATCTATGA GCAGATCTTC CGCTGCCTGC CATCCAATGC CACGCGTTCC 
CTGCGGACTC TCCGGGAGTA CGTGGCCGTG GAGCCCTTGG CCACGGTCAG TCCCCCCTTG 
GCTCGGTCTG AGCTCACCCA GGTCCAGGGC CACCTGGTCC ACTTCCCCCT CAAGTTCCTA 
GAGGATGAGT CTTTGCTGCC CCCGCTGGGT AGCAAGGAGG GCATGATCCC CCTAGAAGTG 
TGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( NM_002663.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002654.3 Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19303
Clone ID Related Accession (Same CDS sequence)	NM_002663.5 , NM_002663.4
Accession Version	NM_002663.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform PLD2A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407587.1, AF033850.2, AF038440.1 and BC015033.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002663.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (PLD2A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF038440.1, BC015033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263088.11/ ENSP00000263088.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGG TTTGCCTTGA GTCTGCGGAA GCACTGCTTC 
GGTGTGATTC TTGGAGCAAA TACCCGGCCA GACTTGGATC TCCGAGACCC CATCTGTGAT 
GACTTCTTCC AGTTGTGGCA AGACATGGCT GAGAGCAACG CCAATATCTA TGAGCAGATC 
TTCCGCTGCC TGCCATCCAA TGCCACGCGT TCCCTGCGGA CTCTCCGGGA GTACGTGGCC 
GTGGAGCCCT TGGCCACGGT CAGTCCCCCC TTGGCTCGGT CTGAGCTCAC CCAGGTCCAG 
GGCCACCTGG TCCACTTCCC CCTCAAGTTC CTAGAGGATG AGTCTTTGCT GCCCCCGCTG 
GGTAGCAAGG AGGGCATGAT CCCCCTAGAA GTGTGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002654.3
CDS	76..2877
Translation	MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	NM_002663.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002663.5

ORF Insert Sequence:

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGG TTTGCCTTGA GTCTGCGGAA GCACTGCTTC 
GGTGTGATTC TTGGAGCAAA TACCCGGCCA GACTTGGATC TCCGAGACCC CATCTGTGAT 
GACTTCTTCC AGTTGTGGCA AGACATGGCT GAGAGCAACG CCAATATCTA TGAGCAGATC 
TTCCGCTGCC TGCCATCCAA TGCCACGCGT TCCCTGCGGA CTCTCCGGGA GTACGTGGCC 
GTGGAGCCCT TGGCCACGGT CAGTCCCCCC TTGGCTCGGT CTGAGCTCAC CCAGGTCCAG 
GGCCACCTGG TCCACTTCCC CCTCAAGTTC CTAGAGGATG AGTCTTTGCT GCCCCCGCTG 
GGTAGCAAGG AGGGCATGAT CCCCCTAGAA GTGTGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( NM_002663.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002654.3 Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.

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CloneID	OHu19303
Clone ID Related Accession (Same CDS sequence)	NM_002663.5 , NM_002663.4
Accession Version	NM_002663.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform PLD2A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA390950.1, DC407587.1, AF033850.2, AF038440.1 and BC015033.1. This sequence is a reference standard in the RefSeqGene project. On Jul 22, 2011 this sequence version replaced NM_002663.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (PLD2A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015033.1, SRR1803612.92039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGG TTTGCCTTGA GTCTGCGGAA GCACTGCTTC 
GGTGTGATTC TTGGAGCAAA TACCCGGCCA GACTTGGATC TCCGAGACCC CATCTGTGAT 
GACTTCTTCC AGTTGTGGCA AGACATGGCT GAGAGCAACG CCAATATCTA TGAGCAGATC 
TTCCGCTGCC TGCCATCCAA TGCCACGCGT TCCCTGCGGA CTCTCCGGGA GTACGTGGCC 
GTGGAGCCCT TGGCCACGGT CAGTCCCCCC TTGGCTCGGT CTGAGCTCAC CCAGGTCCAG 
GGCCACCTGG TCCACTTCCC CCTCAAGTTC CTAGAGGATG AGTCTTTGCT GCCCCCGCTG 
GGTAGCAAGG AGGGCATGAT CCCCCTAGAA GTGTGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002654.3
CDS	127..2928
Translation	MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	NM_002663.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002663.4

ORF Insert Sequence:

ATGACGGCGA CCCCTGAGAG CCTCTTCCCC ACTGGGGACG AACTGGACTC CAGCCAGCTC 
CAGATGGAGT CCGATGAGGT GGACACCCTG AAGGAGGGAG AGGACCCAGC CGACCGGATG 
CACCCGTTTC TGGCCATCTA TGAGCTTCAG TCTCTGAAAG TGCACCCCTT GGTGTTCGCA 
CCTGGGGTCC CTGTCACAGC CCAGGTGGTG GGCACCGAAA GATATACCAG CGGATCCAAG 
GTGGGAACCT GCACTCTGTA TTCTGTCCGC TTGACTCACG GCGACTTTTC CTGGACAACC 
AAGAAGAAAT ACCGTCATTT TCAGGAGCTG CATCGGGACC TCCTGAGACA CAAAGTCTTG 
ATGAGTCTGC TCCCTCTGGC TCGATTTGCC GTTGCCTATT CTCCAGCCCG AGATGCAGGC 
AACAGAGAGA TGCCCTCTCT ACCCCGGGCA GGTCCTGAGG GCTCCACCAG ACATGCAGCC 
AGCAAACAGA AATACCTGGA GAATTACCTC AACCGTCTCT TGACCATGTC TTTCTATCGC 
AACTACCATG CCATGACAGA GTTCCTGGAA GTCAGTCAGC TGTCCTTTAT CCCGGACTTG 
GGCCGCAAAG GACTGGAGGG GATGATCCGG AAGCGCTCAG GTGGCCACCG TGTTCCTGGC 
CTCACCTGCT GTGGCCGAGA CCAAGTTTGT TATCGCTGGT CCAAGAGGTG GCTGGTGGTG 
AAGGACTCCT TCCTGCTGTA CATGTGCCTC GAGACAGGTG CCATCTCATT TGTTCAGCTC 
TTTGACCCTG GCTTTGAGGT GCAAGTGGGG AAAAGGAGCA CGGAGGCACG GCACGGCGTG 
CGGATCGATA CCTCCCACAG GTCCTTGATT CTCAAGTGCA GCAGCTACCG GCAGGCACGG 
TGGTGGGCCC AAGAGATCAC TGAGCTGGCA CAGGGCCCAG GCAGAGACTT CCTACAGCTG 
CACCGGCATG ACAGCTACGC CCCACCCCGG CCTGGGACCT TGGCCCGGTG GTTTGTGAAT 
GGGGCAGGTT ACTTTGCTGC TGTGGCAGAT GCCATCCTTC GAGCTCAAGA GGAGATTTTC 
ATCACAGACT GGTGGTTGAG TCCTGAGGTT TACCTGAAGC GTCCGGCCCA TTCAGATGAC 
TGGAGACTGG ACATTATGCT CAAGAGGAAG GCGGAGGAGG GTGTCCGTGT GTCTATTCTG 
CTGTTTAAAG AAGTGGAATT GGCCTTGGGC ATCAACAGTG GCTATAGCAA GAGGGCGCTG 
ATGCTGCTGC ACCCCAACAT AAAGGTGATG CGTCACCCAG ACCAAGTGAC GTTGTGGGCC 
CATCATGAGA AGCTCCTGGT GGTGGACCAA GTGGTAGCAT TCCTGGGGGG ACTGGACCTT 
GCCTATGGCC GCTGGGATGA CCTGCACTAC CGACTGACTG ACCTTGGAGA CTCCTCTGAA 
TCAGCTGCCT CCCAGCCTCC CACCCCGCGC CCAGACTCAC CAGCCACCCC AGACCTCTCT 
CACAACCAAT TCTTCTGGCT GGGCAAGGAC TACAGCAATC TTATCACCAA GGACTGGGTG 
CAGCTGGACC GGCCTTTCGA AGATTTCATT GACAGGGAGA CGACCCCTCG GATGCCATGG 
CGGGACGTTG GGGTGGTCGT CCATGGCCTA CCGGCCCGGG ACCTTGCCCG GCACTTCATC 
CAGCGCTGGA ACTTCACCAA GACCACCAAG GCCAAGTACA AGACTCCCAC ATACCCCTAC 
CTGCTTCCCA AGTCTACCAG CACGGCCAAT CAGCTCCCCT TCACACTTCC AGGAGGGCAG 
TGCACCACCG TACAGGTCTT GCGATCAGTG GACCGCTGGT CAGCAGGGAC TCTGGAGAAC 
TCCATCCTCA ATGCCTACCT GCACACCATC AGGGAGAGCC AGCACTTCCT CTACATTGAG 
AATCAGTTCT TCATTAGCTG CTCAGATGGG CGGACGGTTC TGAACAAGGT GGGCGATGAG 
ATTGTGGACA GAATCCTGAA GGCCCACAAA CAGGGGTGGT GTTACCGAGT CTACGTGCTT 
TTGCCCTTAC TCCCTGGCTT CGAGGGTGAC ATCTCCACGG GCGGTGGCAA CTCCATCCAG 
GCCATTCTGC ACTTTACTTA CAGGACCCTG TGTCGTGGGG AGTATTCAAT CCTGCATCGC 
CTTAAAGCAG CCATGGGGAC AGCATGGCGG GACTATATTT CCATCTGCGG GCTTCGTACA 
CACGGAGAGC TGGGCGGGCA CCCCGTCTCG GAGCTCATCT ACATCCACAG CAAGGTGCTC 
ATCGCAGATG ACCGGACAGT CATCATTGGT TCTGCAAACA TCAATGACCG GAGCTTGCTG 
GGGAAGCGGG ACAGTGAGCT GGCCGTGCTG ATCGAGGACA CAGAGACGGA ACCATCCCTC 
ATGAATGGGG CAGAGTATCA GGCGGGCAGG TTTGCCTTGA GTCTGCGGAA GCACTGCTTC 
GGTGTGATTC TTGGAGCAAA TACCCGGCCA GACTTGGATC TCCGAGACCC CATCTGTGAT 
GACTTCTTCC AGTTGTGGCA AGACATGGCT GAGAGCAACG CCAATATCTA TGAGCAGATC 
TTCCGCTGCC TGCCATCCAA TGCCACGCGT TCCCTGCGGA CTCTCCGGGA GTACGTGGCC 
GTGGAGCCCT TGGCCACGGT CAGTCCCCCC TTGGCTCGGT CTGAGCTCAC CCAGGTCCAG 
GGCCACCTGG TCCACTTCCC CCTCAAGTTC CTAGAGGATG AGTCTTTGCT GCCCCCGCTG 
GGTAGCAAGG AGGGCATGAT CCCCCTAGAA GTGTGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( XM_017024764.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880253.1 Homo sapiens phospholipase D2 (PLD2), transcript variant X7, mRNA.

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CloneID	OHu86927
Clone ID Related Accession (Same CDS sequence)	XM_017024764.2 , XM_017024764.1
Accession Version	XM_017024764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGTGTGAT TCTTGGAGCA AATACCCGGC CAGACTTGGA TCTCCGAGAC 
CCCATCTGTG ATGACTTCTT CCAGTTGTGG CAAGACATGG CTGAGAGCAA CGCCAATATC 
TATGAGCAGA TCTTCCGCTG CCTGCCATCC AATGCCACGC GTTCCCTGCG GACTCTCCGG 
GAGTACGTGG CCGTGGAGCC CTTGGCCACG GTCAGTCCCC CCTTGGCTCG GTCTGAGCTC 
ACCCAGGTCC AGGGCCACCT GGTCCACTTC CCCCTCAAGT TCCTAGAGGA TGAGTCTTTG 
CTGCCCCCGC TGGGTAGCAA GGAGGGCATG ATCCCCCTAG AAGTGTGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880253.1
CDS	1269..2882
Translation	MLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGSVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	XM_017024764.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024764.2

ORF Insert Sequence:

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGTGTGAT TCTTGGAGCA AATACCCGGC CAGACTTGGA TCTCCGAGAC 
CCCATCTGTG ATGACTTCTT CCAGTTGTGG CAAGACATGG CTGAGAGCAA CGCCAATATC 
TATGAGCAGA TCTTCCGCTG CCTGCCATCC AATGCCACGC GTTCCCTGCG GACTCTCCGG 
GAGTACGTGG CCGTGGAGCC CTTGGCCACG GTCAGTCCCC CCTTGGCTCG GTCTGAGCTC 
ACCCAGGTCC AGGGCCACCT GGTCCACTTC CCCCTCAAGT TCCTAGAGGA TGAGTCTTTG 
CTGCCCCCGC TGGGTAGCAA GGAGGGCATG ATCCCCCTAG AAGTGTGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( XM_017024764.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880253.1 Homo sapiens phospholipase D2 (PLD2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu86927
Clone ID Related Accession (Same CDS sequence)	XM_017024764.2 , XM_017024764.1
Accession Version	XM_017024764.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phospholipase D2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGTGTGAT TCTTGGAGCA AATACCCGGC CAGACTTGGA TCTCCGAGAC 
CCCATCTGTG ATGACTTCTT CCAGTTGTGG CAAGACATGG CTGAGAGCAA CGCCAATATC 
TATGAGCAGA TCTTCCGCTG CCTGCCATCC AATGCCACGC GTTCCCTGCG GACTCTCCGG 
GAGTACGTGG CCGTGGAGCC CTTGGCCACG GTCAGTCCCC CCTTGGCTCG GTCTGAGCTC 
ACCCAGGTCC AGGGCCACCT GGTCCACTTC CCCCTCAAGT TCCTAGAGGA TGAGTCTTTG 
CTGCCCCCGC TGGGTAGCAA GGAGGGCATG ATCCCCCTAG AAGTGTGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880253.1
CDS	1282..2895
Translation	MLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGSVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Target ORF information:

RefSeq Version	XM_017024764.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D2 (PLD2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024764.1

ORF Insert Sequence:

ATGCTCAAGA GGAAGGCGGA GGAGGGTGTC CGTGTGTCTA TTCTGCTGTT TAAAGAAGTG 
GAATTGGCCT TGGGCATCAA CAGTGGCTAT AGCAAGAGGG CGCTGATGCT GCTGCACCCC 
AACATAAAGG TGATGCGTCA CCCAGACCAA GTGACGTTGT GGGCCCATCA TGAGAAGCTC 
CTGGTGGTGG ACCAAGTGGT AGCATTCCTG GGGGGACTGG ACCTTGCCTA TGGCCGCTGG 
GATGACCTGC ACTACCGACT GACTGACCTT GGAGACTCCT CTGAATCAGC TGCCTCCCAG 
CCTCCCACCC CGCGCCCAGA CTCACCAGCC ACCCCAGACC TCTCTCACAA CCAATTCTTC 
TGGCTGGGCA AGGACTACAG CAATCTTATC ACCAAGGACT GGGTGCAGCT GGACCGGCCT 
TTCGAAGATT TCATTGACAG GGAGACGACC CCTCGGATGC CATGGCGGGA CGTTGGGGTG 
GTCGTCCATG GCCTACCGGC CCGGGACCTT GCCCGGCACT TCATCCAGCG CTGGAACTTC 
ACCAAGACCA CCAAGGCCAA GTACAAGACT CCCACATACC CCTACCTGCT TCCCAAGTCT 
ACCAGCACGG CCAATCAGCT CCCCTTCACA CTTCCAGGAG GGCAGTGCAC CACCGTACAG 
GTCTTGCGAT CAGTGGACCG CTGGTCAGCA GGGACTCTGG AGAACTCCAT CCTCAATGCC 
TACCTGCACA CCATCAGGGA GAGCCAGCAC TTCCTCTACA TTGAGAATCA GTTCTTCATT 
AGCTGCTCAG ATGGGCGGAC GGTTCTGAAC AAGGTGGGCG ATGAGATTGT GGACAGAATC 
CTGAAGGCCC ACAAACAGGG GTGGTGTTAC CGAGTCTACG TGCTTTTGCC CTTACTCCCT 
GGCTTCGAGG GTGACATCTC CACGGGCGGT GGCAACTCCA TCCAGGCCAT TCTGCACTTT 
ACTTACAGGA CCCTGTGTCG TGGGGAGTAT TCAATCCTGC ATCGCCTTAA AGCAGCCATG 
GGGACAGCAT GGCGGGACTA TATTTCCATC TGCGGGCTTC GTACACACGG AGAGCTGGGC 
GGGCACCCCG TCTCGGAGCT CATCTACATC CACAGCAAGG TGCTCATCGC AGATGACCGG 
ACAGTCATCA TTGGTTCTGC AAACATCAAT GACCGGAGCT TGCTGGGGAA GCGGGACAGT 
GAGCTGGCCG TGCTGATCGA GGACACAGAG ACGGAACCAT CCCTCATGAA TGGGGCAGAG 
TATCAGGCGG GCAGTGTGAT TCTTGGAGCA AATACCCGGC CAGACTTGGA TCTCCGAGAC 
CCCATCTGTG ATGACTTCTT CCAGTTGTGG CAAGACATGG CTGAGAGCAA CGCCAATATC 
TATGAGCAGA TCTTCCGCTG CCTGCCATCC AATGCCACGC GTTCCCTGCG GACTCTCCGG 
GAGTACGTGG CCGTGGAGCC CTTGGCCACG GTCAGTCCCC CCTTGGCTCG GTCTGAGCTC 
ACCCAGGTCC AGGGCCACCT GGTCCACTTC CCCCTCAAGT TCCTAGAGGA TGAGTCTTTG 
CTGCCCCCGC TGGGTAGCAA GGAGGGCATG ATCCCCCTAG AAGTGTGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway.
Scientific reports7(1)9112(2017 Aug)
Nelson RK,Ya-Ping J,Gadbery J,Abedeen D,Sampson N,Lin RZ,Frohman MA

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PLD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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