PLCG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCG1
Entrez Gene ID	5335
Full Name	phospholipase C gamma 1
Synonyms	NCKAP3,PLC-II,PLC1,PLC148,PLCgamma1
General protein information	Preferred Names phospholipase C gamma 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate PLC-148 PLC-gamma-1 inositoltrisphosphohydrolase monophosphatidylinositol phosphodiesterase phosphatidylinositol phospholipase C phosphoinositidase C phosphoinositide phospholipase C phosphoinositide phospholipase C-gamma-1 phospholipase C, gamma 1 (formerly subtype 148) phospholipase C-148 phospholipase C-II triphosphoinositide phosphodiesterase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q12
Summary	The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	172420

mRNA and Protein(s)

mRNA	Protein	Name
NM_182811.1	NP_877963.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b
XM_005260438.2	XP_005260495.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X1
NM_002660.2	NP_002651.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
XM_011528865.1	XP_011527167.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X2
XM_011528867.2	XP_011527169.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X3
NM_182811.2	NP_877963.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b
NM_002660.3	NP_002651.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Plcg1	NP_037319.1
Pan troglodytes (chimpanzee)	PLCG1	XP_514650.3
Bos taurus (cattle)	PLCG1	NP_776850.1
Drosophila melanogaster (fruit fly)	sl	NP_476726.2
Mus musculus (house mouse)	Plcg1	NP_067255.2
Homo sapiens (human)	PLCG1	NP_002651.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011152	XP_309496.4
Caenorhabditis elegans (roundworm)	plc-3	NP_496205.2
	PLCG1	XP_001087295.2
Canis lupus familiaris (dog)	PLCG1	XP_005635117.1
Gallus gallus (chicken)	PLCG1	XP_004947069.1
Danio rerio (zebrafish)	plcg1	NP_919388.1
Arabidopsis thaliana (thale cress)	PLC2	NP_187464.1
Arabidopsis thaliana (thale cress)	AT3G55940	NP_191153.1

Gene Ontology
Bibliography

Related articles in PubMed

Interplay between FGFR2b-induced autophagy and phagocytosis: role of PLC?-mediated signalling.
Nanni M, Ranieri D, Raffa S, Torrisi MR, Belleudi F
Journal of cellular and molecular medicine22(1)668-683(2018 Jan)

Mutant FGFR3 associated with SADDAN disease causes cytoskeleton disorganization through PLC?1/Src-mediated paxillin hyperphosphorylation.
Montone R, Romanelli MG, Baruzzi A, Ferrarini F, Liboi E, Lievens PM
The international journal of biochemistry & cell biology9517-26(2017 Dec)

PLC?1: Potential arbitrator of cancer progression.
Jang HJ, Suh PG, Lee YJ, Shin KJ, Cocco L, Chae YC
Advances in biological regulation(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Syk-induced signals in bone marrow stromal cell lines are mediated by phospholipase C gamma1 (PLCgamma1) in osteogenesis and PLCgamma2 in adipogenesis.
Title: Spleen tyrosine kinase influences the early stages of multilineage differentiation of bone marrow stromal cell lines by regulating phospholipase C gamma activities.

1,25(OH)2D3 indirectly modulates the differentiation of Treg/Th17 cells by a ff ;ecting the VDR/PLC-gamma1/TGF-beta1pathway. These results indicate that administration 1,25(OH)2D3 supplements may be a beneficial treatment for organ transplantation recipients.
Title: 1,25(OH)

Results show that PLCgamma-1 activation enhanced skin cell transformation.
Title: UCP2 upregulation promotes PLC?-1 signaling during skin cell transformation.

High PLC gamma expression is associated with breast cancer.
Title: Expression of phospholipase C isozymes in human breast cancer and their clinical significance.

The products of PLC-gamma activity mediate the innate immune response by regulating respiratory burst, phagocytosis, cell adhesion, and cell migration. (Review)
Title: Phospholipase C? in Toll-like receptor-mediated inflammation and innate immunity.

The following PLCG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26839	NM_002660.3 Latest version!	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu59764	XM_011528865.1 Latest version!	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu27177	NM_182811.2 Latest version!	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu53022	XM_005260438.2 Latest version!	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu59765	XM_011528867.2 Latest version!	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu27177	NM_182811.1	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu26839	NM_002660.2	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCG1 ( NM_002660.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002651.2 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26839
Clone ID Related Accession (Same CDS sequence)	NM_002660.2 , NM_002660.3
Accession Version	NM_002660.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB210028.1, M34667.1 and AL110247.1. On Aug 12, 2003 this sequence version replaced NM_002660.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.116059.1, SRR1803615.56084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002651.2
CDS	122..3997
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	NM_002660.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002660.2

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( NM_002660.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002651.2 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26839
Clone ID Related Accession (Same CDS sequence)	NM_002660.2 , NM_002660.3
Accession Version	NM_002660.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB210028.1, M34667.1, AL110247.1 and AL022394.3. On Jun 2, 2019 this sequence version replaced NM_002660.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.56084.1, SRR1803611.150658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002651.2
CDS	100..3975
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	NM_002660.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002660.3

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( XM_011528865.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527167.1 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X2, mRNA.

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CloneID	OHu59764
Clone ID Related Accession (Same CDS sequence)	XM_011528865.1
Accession Version	XM_011528865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCC AGGTCAACTA CCGGGTCCCC AACATGCGCT TCCTCCGAGA GCGGCTGACG 
GACCTGGAGC AGCGCAGCGG GGACATCACC TACGGGCAGT TTGCTCAGCT GTACCGCAGC 
CTCATGTACA GCGCCCAGAA GACGATGGAC CTCCCCTTCT TGGAAGCCAG TACTCTGAGG 
GCTGGGGAGC GGCCGGAGCT TTGCCGAGTG TCCCTTCCTG AGTTCCAGCA GTTCCTTCTT 
GACTACCAGG GGGAGCTGTG GGCTGTTGAT CGCCTCCAGG TGCAGGAGTT CATGCTCAGC 
TTCCTCCGAG ACCCCTTACG AGAGATCGAG GAGCCATACT TCTTCCTGGA TGAGTTTGTC 
ACCTTCCTGT TCTCCAAAGA GAACAGTGTG TGGAACTCGC AGCTGGATGC AGTATGCCCG 
GACACCATGA ACAACCCTCT TTCCCACTAC TGGATCTCCT CCTCGCACAA CACGTACCTG 
ACCGGGGACC AGTTCTCCAG TGAGTCCTCC TTGGAAGCCT ATGCTCGCTG CCTGCGGATG 
GGCTGTCGCT GCATTGAGTT GGACTGCTGG GACGGCCCGG ATGGGATGCC AGTTATTTAC 
CATGGGCACA CCCTTACCAC CAAGATCAAG TTCTCAGATG TCCTGCACAC CATCAAGGAG 
CATGCCTTTG TGGCCTCAGA GTACCCAGTC ATCCTGTCCA TTGAGGACCA CTGCAGCATT 
GCCCAGCAGA GAAACATGGC CCAATACTTC AAGAAGGTGC TGGGGGACAC ACTCCTCACC 
AAGCCCGTGG AGATCTCTGC CGACGGGCTC CCCTCACCCA ACCAGCTTAA GAGGAAGATC 
CTCATCAAGC ACAAGAAGCT GGCTGAGGGC AGTGCCTACG AGGAGGTGCC TACATCCATG 
ATGTACTCTG AGAACGACAT CAGCAACTCT ATCAAGAATG GCATCCTCTA CCTGGAGGAC 
CCTGTGAACC ACGAATGGTA TCCCCACTAC TTTGTTCTGA CCAGCAGCAA GATCTACTAC 
TCTGAGGAGA CCAGCAGTGA CCAGGGCAAC GAGGATGAGG AGGAGCCCAA GGAGGTCAGC 
AGCAGCACAG AGCTGCACTC CAATGAGAAG TGGTTCCATG GGAAGCTAGG GGCAGGGCGT 
GACGGGCGTC ACATCGCTGA GCGCCTGCTT ACTGAGTACT GCATCGAGAC CGGAGCCCCT 
GACGGCTCCT TCCTCGTGCG AGAGAGTGAG ACCTTCGTGG GCGACTACAC GCTCTCTTTC 
TGGCGGAACG GGAAAGTCCA GCACTGCCGT ATCCACTCCC GGCAAGATGC TGGGACCCCC 
AAGTTCTTCT TGACAGACAA CCTCGTCTTT GACTCCCTCT ATGACCTCAT CACGCACTAC 
CAGCAGGTGC CCCTGCGCTG TAATGAGTTT GAGATGCGAC TTTCAGAGCC TGTCCCACAG 
ACCAACGCCC ACGAGAGCAA AGAGTGGTAC CACGCGAGCC TGACCAGAGC ACAGGCTGAG 
CACATGCTAA TGCGCGTCCC TCGTGATGGG GCCTTCCTGG TGCGGAAGCG GAATGAACCC 
AACTCATATG CCATCTCTTT CCGGGCTGAG GGCAAGATCA AGCATTGCCG TGTCCAGCAA 
GAGGGCCAGA CAGTGATGCT AGGGAACTCG GAGTTCGACA GCCTTGTTGA CCTCATCAGC 
TACTATGAGA AACACCCGCT ATACCGCAAG ATGAAGCTGC GCTATCCCAT CAACGAGGAG 
GCACTGGAGA AGATTGGCAC AGCTGAGCCT GACTACGGGG CCCTGTATGA GGGACGCAAC 
CCTGGCTTCT ATGTAGAGGC AAACCCTATG CCAACTTTCA AGTGTGCAGT CAAAGCCCTC 
TTTGACTACA AGGCCCAGAG GGAGGACGAG CTGACCTTCA TCAAGAGCGC CATCATCCAG 
AATGTGGAGA AGCAAGAGGG AGGCTGGTGG CGAGGGGACT ACGGAGGGAA GAAGCAGCTG 
TGGTTCCCAT CAAACTACGT GGAAGAGATG GTCAACCCCG TGGCCCTGGA GCCGGAGAGG 
GAGCACTTGG ACGAGAACAG CCCCCTAGGG GACTTGCTGC GGGGGGTCTT GGATGTGCCG 
GCTTGTCAGA TTGCCATCCG TCCTGAGGGC AAGAACAACC GGCTCTTCGT CTTCTCCATC 
AGCATGGCGT CGGTGGCCCA CTGGTCCCTG GATGTTGCTG CCGACTCACA GGAGGAGCTG 
CAGGACTGGG TGAAAAAGAT CCGTGAAGTG GCCCAGACAG CAGACGCCAG GCTCACTGAA 
GGGAAGATAA TGGAACGGAG GAAGAAGATT GCCCTGGAGC TCTCTGAACT TGTCGTCTAC 
TGCCGGCCTG TTCCCTTTGA TGAAGAGAAG ATTGGCACAG AACGTGCTTG CTACCGGGAC 
ATGTCATCCT TCCCGGAAAC CAAGGCTGAG AAATACGTGA ACAAGGCCAA AGGCAAGAAG 
TTCCTTCAGT ACAATCGACT GCAGCTCTCC CGCATCTACC CCAAGGGCCA GCGACTGGAT 
TCCTCCAACT ACGATCCTTT GCCCATGTGG ATCTGTGGCA GTCAGCTTGT GGCCCTCAAC 
TTCCAGACCC CTGACAAGCC TATGCAGATG AACCAGGCCC TCTTCATGAC GGGCAGGCAC 
TGTGGCTACG TGCTGCAGCC AAGCACCATG CGGGATGAGG CCTTCGACCC CTTTGACAAG 
AGCAGCCTCC GCGGGCTGGA GCCATGTGCC ATCTCTATTG AGGTGCTGGG GGCCCGACAT 
CTGCCAAAGA ATGGCCGAGG CATTGTGTGT CCTTTTGTGG AGATTGAGGT GGCTGGAGCT 
GAGTATGACA GCACCAAGCA GAAGACAGAG TTTGTGGGTC AGTCTGTCTT CCCAGTCATC 
CTCCTCATCC TGCTGGGGCA CTGCAAGCCT CTCCCCACCA GTCATCCCAT CCTCTCCCAC 
GGTGACCTGA AGCCTTTTGT CGTTGCCTTC ACAGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527167.1
CDS	526..3981
Translation	MLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVGQSVFPVILLILLGHCKPLPTSHPILSHGDLKPFVVAFTVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	XM_011528865.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528865.1

ORF Insert Sequence:

ATGCTGTCCC AGGTCAACTA CCGGGTCCCC AACATGCGCT TCCTCCGAGA GCGGCTGACG 
GACCTGGAGC AGCGCAGCGG GGACATCACC TACGGGCAGT TTGCTCAGCT GTACCGCAGC 
CTCATGTACA GCGCCCAGAA GACGATGGAC CTCCCCTTCT TGGAAGCCAG TACTCTGAGG 
GCTGGGGAGC GGCCGGAGCT TTGCCGAGTG TCCCTTCCTG AGTTCCAGCA GTTCCTTCTT 
GACTACCAGG GGGAGCTGTG GGCTGTTGAT CGCCTCCAGG TGCAGGAGTT CATGCTCAGC 
TTCCTCCGAG ACCCCTTACG AGAGATCGAG GAGCCATACT TCTTCCTGGA TGAGTTTGTC 
ACCTTCCTGT TCTCCAAAGA GAACAGTGTG TGGAACTCGC AGCTGGATGC AGTATGCCCG 
GACACCATGA ACAACCCTCT TTCCCACTAC TGGATCTCCT CCTCGCACAA CACGTACCTG 
ACCGGGGACC AGTTCTCCAG TGAGTCCTCC TTGGAAGCCT ATGCTCGCTG CCTGCGGATG 
GGCTGTCGCT GCATTGAGTT GGACTGCTGG GACGGCCCGG ATGGGATGCC AGTTATTTAC 
CATGGGCACA CCCTTACCAC CAAGATCAAG TTCTCAGATG TCCTGCACAC CATCAAGGAG 
CATGCCTTTG TGGCCTCAGA GTACCCAGTC ATCCTGTCCA TTGAGGACCA CTGCAGCATT 
GCCCAGCAGA GAAACATGGC CCAATACTTC AAGAAGGTGC TGGGGGACAC ACTCCTCACC 
AAGCCCGTGG AGATCTCTGC CGACGGGCTC CCCTCACCCA ACCAGCTTAA GAGGAAGATC 
CTCATCAAGC ACAAGAAGCT GGCTGAGGGC AGTGCCTACG AGGAGGTGCC TACATCCATG 
ATGTACTCTG AGAACGACAT CAGCAACTCT ATCAAGAATG GCATCCTCTA CCTGGAGGAC 
CCTGTGAACC ACGAATGGTA TCCCCACTAC TTTGTTCTGA CCAGCAGCAA GATCTACTAC 
TCTGAGGAGA CCAGCAGTGA CCAGGGCAAC GAGGATGAGG AGGAGCCCAA GGAGGTCAGC 
AGCAGCACAG AGCTGCACTC CAATGAGAAG TGGTTCCATG GGAAGCTAGG GGCAGGGCGT 
GACGGGCGTC ACATCGCTGA GCGCCTGCTT ACTGAGTACT GCATCGAGAC CGGAGCCCCT 
GACGGCTCCT TCCTCGTGCG AGAGAGTGAG ACCTTCGTGG GCGACTACAC GCTCTCTTTC 
TGGCGGAACG GGAAAGTCCA GCACTGCCGT ATCCACTCCC GGCAAGATGC TGGGACCCCC 
AAGTTCTTCT TGACAGACAA CCTCGTCTTT GACTCCCTCT ATGACCTCAT CACGCACTAC 
CAGCAGGTGC CCCTGCGCTG TAATGAGTTT GAGATGCGAC TTTCAGAGCC TGTCCCACAG 
ACCAACGCCC ACGAGAGCAA AGAGTGGTAC CACGCGAGCC TGACCAGAGC ACAGGCTGAG 
CACATGCTAA TGCGCGTCCC TCGTGATGGG GCCTTCCTGG TGCGGAAGCG GAATGAACCC 
AACTCATATG CCATCTCTTT CCGGGCTGAG GGCAAGATCA AGCATTGCCG TGTCCAGCAA 
GAGGGCCAGA CAGTGATGCT AGGGAACTCG GAGTTCGACA GCCTTGTTGA CCTCATCAGC 
TACTATGAGA AACACCCGCT ATACCGCAAG ATGAAGCTGC GCTATCCCAT CAACGAGGAG 
GCACTGGAGA AGATTGGCAC AGCTGAGCCT GACTACGGGG CCCTGTATGA GGGACGCAAC 
CCTGGCTTCT ATGTAGAGGC AAACCCTATG CCAACTTTCA AGTGTGCAGT CAAAGCCCTC 
TTTGACTACA AGGCCCAGAG GGAGGACGAG CTGACCTTCA TCAAGAGCGC CATCATCCAG 
AATGTGGAGA AGCAAGAGGG AGGCTGGTGG CGAGGGGACT ACGGAGGGAA GAAGCAGCTG 
TGGTTCCCAT CAAACTACGT GGAAGAGATG GTCAACCCCG TGGCCCTGGA GCCGGAGAGG 
GAGCACTTGG ACGAGAACAG CCCCCTAGGG GACTTGCTGC GGGGGGTCTT GGATGTGCCG 
GCTTGTCAGA TTGCCATCCG TCCTGAGGGC AAGAACAACC GGCTCTTCGT CTTCTCCATC 
AGCATGGCGT CGGTGGCCCA CTGGTCCCTG GATGTTGCTG CCGACTCACA GGAGGAGCTG 
CAGGACTGGG TGAAAAAGAT CCGTGAAGTG GCCCAGACAG CAGACGCCAG GCTCACTGAA 
GGGAAGATAA TGGAACGGAG GAAGAAGATT GCCCTGGAGC TCTCTGAACT TGTCGTCTAC 
TGCCGGCCTG TTCCCTTTGA TGAAGAGAAG ATTGGCACAG AACGTGCTTG CTACCGGGAC 
ATGTCATCCT TCCCGGAAAC CAAGGCTGAG AAATACGTGA ACAAGGCCAA AGGCAAGAAG 
TTCCTTCAGT ACAATCGACT GCAGCTCTCC CGCATCTACC CCAAGGGCCA GCGACTGGAT 
TCCTCCAACT ACGATCCTTT GCCCATGTGG ATCTGTGGCA GTCAGCTTGT GGCCCTCAAC 
TTCCAGACCC CTGACAAGCC TATGCAGATG AACCAGGCCC TCTTCATGAC GGGCAGGCAC 
TGTGGCTACG TGCTGCAGCC AAGCACCATG CGGGATGAGG CCTTCGACCC CTTTGACAAG 
AGCAGCCTCC GCGGGCTGGA GCCATGTGCC ATCTCTATTG AGGTGCTGGG GGCCCGACAT 
CTGCCAAAGA ATGGCCGAGG CATTGTGTGT CCTTTTGTGG AGATTGAGGT GGCTGGAGCT 
GAGTATGACA GCACCAAGCA GAAGACAGAG TTTGTGGGTC AGTCTGTCTT CCCAGTCATC 
CTCCTCATCC TGCTGGGGCA CTGCAAGCCT CTCCCCACCA GTCATCCCAT CCTCTCCCAC 
GGTGACCTGA AGCCTTTTGT CGTTGCCTTC ACAGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGCAGGA GAATGGTGAC 
CTCAGTCCCT TCAGTGGTAC GTCCCTGCGG GAGCGGGGCT CAGATGCCTC AGGCCAGCTG 
TTTCATGGCC GAGCCCGGGA AGGCTCCTTT GAATCCCGCT ACCAGCAGCC GTTTGAGGAC 
TTCCGCATCT CCCAGGAGCA TCTCGCAGAC CATTTTGACA GTCGAGAACG AAGGGCCCCA 
AGAAGGACTC GGGTCAATGG AGACAACCGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( NM_182811.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_877963.1 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.

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CloneID	OHu27177
Clone ID Related Accession (Same CDS sequence)	NM_182811.1 , NM_182811.2
Accession Version	NM_182811.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB210028.1 and M34667.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB210028.1, BC136466.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877963.1
CDS	122..3994
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	NM_182811.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182811.1

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( NM_182811.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_877963.1 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.

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CloneID	OHu27177
Clone ID Related Accession (Same CDS sequence)	NM_182811.1 , NM_182811.2
Accession Version	NM_182811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB210028.1, M34667.1 and AL022394.3. On Jun 2, 2019 this sequence version replaced NM_182811.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB210028.1, BC136466.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877963.1
CDS	100..3972
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	NM_182811.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182811.2

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGTGGACA ATGGACTCAA CCCTGTATGG 
CCAGCCAAGC CCTTCCACTT CCAGATCAGT AACCCTGAAT TTGCCTTTCT GCGCTTCGTG 
GTGTATGAGG AAGACATGTT TAGTGACCAG AATTTCCTGG CTCAGGCTAC TTTCCCAGTA 
AAAGGCCTGA AGACAGGATA CAGAGCAGTG CCTTTGAAGA ACAACTACAG TGAGGACCTG 
GAGTTGGCCT CCCTGCTGAT CAAGATTGAC ATTTTCCCTG CCAAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( XM_005260438.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260495.1 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X1, mRNA.

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CloneID	OHu53022
Clone ID Related Accession (Same CDS sequence)	XM_005260438.2
Accession Version	XM_005260438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005260438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGGTCAGT CTGTCTTCCC AGTCATCCTC 
CTCATCCTGC TGGGGCACTG CAAGCCTCTC CCCACCAGTC ATCCCATCCT CTCCCACGGT 
GACCTGAAGC CTTTTGTCGT TGCCTTCACA GTGGACAATG GACTCAACCC TGTATGGCCA 
GCCAAGCCCT TCCACTTCCA GATCAGTAAC CCTGAATTTG CCTTTCTGCG CTTCGTGGTG 
TATGAGGAAG ACATGTTTAG TGACCAGAAT TTCCTGGCTC AGGCTACTTT CCCAGTAAAA 
GGCCTGAAGA CAGGATACAG AGCAGTGCCT TTGAAGAACA ACTACAGTGA GGACCTGGAG 
TTGGCCTCCC TGCTGATCAA GATTGACATT TTCCCTGCCA AGCAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260495.1
CDS	124..4116
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVGQSVFPVILLILLGHCKPLPTSHPILSHGDLKPFVVAFTVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Target ORF information:

RefSeq Version	XM_005260438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260438.2

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CCATCCGTCC TGAGGGCAAG AACAACCGGC TCTTCGTCTT CTCCATCAGC 
ATGGCGTCGG TGGCCCACTG GTCCCTGGAT GTTGCTGCCG ACTCACAGGA GGAGCTGCAG 
GACTGGGTGA AAAAGATCCG TGAAGTGGCC CAGACAGCAG ACGCCAGGCT CACTGAAGGG 
AAGATAATGG AACGGAGGAA GAAGATTGCC CTGGAGCTCT CTGAACTTGT CGTCTACTGC 
CGGCCTGTTC CCTTTGATGA AGAGAAGATT GGCACAGAAC GTGCTTGCTA CCGGGACATG 
TCATCCTTCC CGGAAACCAA GGCTGAGAAA TACGTGAACA AGGCCAAAGG CAAGAAGTTC 
CTTCAGTACA ATCGACTGCA GCTCTCCCGC ATCTACCCCA AGGGCCAGCG ACTGGATTCC 
TCCAACTACG ATCCTTTGCC CATGTGGATC TGTGGCAGTC AGCTTGTGGC CCTCAACTTC 
CAGACCCCTG ACAAGCCTAT GCAGATGAAC CAGGCCCTCT TCATGACGGG CAGGCACTGT 
GGCTACGTGC TGCAGCCAAG CACCATGCGG GATGAGGCCT TCGACCCCTT TGACAAGAGC 
AGCCTCCGCG GGCTGGAGCC ATGTGCCATC TCTATTGAGG TGCTGGGGGC CCGACATCTG 
CCAAAGAATG GCCGAGGCAT TGTGTGTCCT TTTGTGGAGA TTGAGGTGGC TGGAGCTGAG 
TATGACAGCA CCAAGCAGAA GACAGAGTTT GTGGGTCAGT CTGTCTTCCC AGTCATCCTC 
CTCATCCTGC TGGGGCACTG CAAGCCTCTC CCCACCAGTC ATCCCATCCT CTCCCACGGT 
GACCTGAAGC CTTTTGTCGT TGCCTTCACA GTGGACAATG GACTCAACCC TGTATGGCCA 
GCCAAGCCCT TCCACTTCCA GATCAGTAAC CCTGAATTTG CCTTTCTGCG CTTCGTGGTG 
TATGAGGAAG ACATGTTTAG TGACCAGAAT TTCCTGGCTC AGGCTACTTT CCCAGTAAAA 
GGCCTGAAGA CAGGATACAG AGCAGTGCCT TTGAAGAACA ACTACAGTGA GGACCTGGAG 
TTGGCCTCCC TGCTGATCAA GATTGACATT TTCCCTGCCA AGCAGGAGAA TGGTGACCTC 
AGTCCCTTCA GTGGTACGTC CCTGCGGGAG CGGGGCTCAG ATGCCTCAGG CCAGCTGTTT 
CATGGCCGAG CCCGGGAAGG CTCCTTTGAA TCCCGCTACC AGCAGCCGTT TGAGGACTTC 
CGCATCTCCC AGGAGCATCT CGCAGACCAT TTTGACAGTC GAGAACGAAG GGCCCCAAGA 
AGGACTCGGG TCAATGGAGA CAACCGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCG1 ( XM_011528867.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527169.1 Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X7, mRNA.

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CloneID	OHu59765
Clone ID Related Accession (Same CDS sequence)	XM_011528867.2
Accession Version	XM_011528867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CATGGCGTCG GTGGCCCACT GGTCCCTGGA TGTTGCTGCC GACTCACAGG 
AGGAGCTGCA GGACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527169.1
CDS	122..2842
Translation	MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAWRRWPTGPWMLLPTHRRSCRTG

Target ORF information:

RefSeq Version	XM_011528867.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528867.2

ORF Insert Sequence:

ATGGCGGGCG CCGCGTCCCC TTGCGCCAAC GGCTGCGGGC CCGGCGCGCC CTCGGACGCC 
GAGGTGCTGC ACCTCTGCCG CAGCCTCGAG GTGGGCACCG TCATGACTTT GTTCTACTCC 
AAGAAGTCGC AGCGACCCGA GCGGAAGACC TTCCAGGTCA AGCTGGAGAC GCGCCAGATC 
ACGTGGAGCC GGGGCGCCGA CAAGATCGAG GGGGCCATTG ACATTCGTGA AATTAAGGAG 
ATCCGCCCAG GGAAGACCTC ACGGGACTTT GATCGCTATC AAGAGGACCC AGCTTTCCGG 
CCGGACCAGT CACATTGCTT TGTCATTCTC TATGGAATGG AATTTCGCCT GAAAACGCTG 
AGCCTGCAAG CCACATCTGA GGATGAAGTG AACATGTGGA TCAAGGGCTT AACTTGGCTG 
ATGGAGGATA CATTGCAGGC ACCCACACCC CTGCAGATTG AGAGGTGGCT CCGGAAGCAG 
TTTTACTCAG TGGATCGGAA TCGTGAGGAT CGTATATCAG CCAAGGACCT GAAGAACATG 
CTGTCCCAGG TCAACTACCG GGTCCCCAAC ATGCGCTTCC TCCGAGAGCG GCTGACGGAC 
CTGGAGCAGC GCAGCGGGGA CATCACCTAC GGGCAGTTTG CTCAGCTGTA CCGCAGCCTC 
ATGTACAGCG CCCAGAAGAC GATGGACCTC CCCTTCTTGG AAGCCAGTAC TCTGAGGGCT 
GGGGAGCGGC CGGAGCTTTG CCGAGTGTCC CTTCCTGAGT TCCAGCAGTT CCTTCTTGAC 
TACCAGGGGG AGCTGTGGGC TGTTGATCGC CTCCAGGTGC AGGAGTTCAT GCTCAGCTTC 
CTCCGAGACC CCTTACGAGA GATCGAGGAG CCATACTTCT TCCTGGATGA GTTTGTCACC 
TTCCTGTTCT CCAAAGAGAA CAGTGTGTGG AACTCGCAGC TGGATGCAGT ATGCCCGGAC 
ACCATGAACA ACCCTCTTTC CCACTACTGG ATCTCCTCCT CGCACAACAC GTACCTGACC 
GGGGACCAGT TCTCCAGTGA GTCCTCCTTG GAAGCCTATG CTCGCTGCCT GCGGATGGGC 
TGTCGCTGCA TTGAGTTGGA CTGCTGGGAC GGCCCGGATG GGATGCCAGT TATTTACCAT 
GGGCACACCC TTACCACCAA GATCAAGTTC TCAGATGTCC TGCACACCAT CAAGGAGCAT 
GCCTTTGTGG CCTCAGAGTA CCCAGTCATC CTGTCCATTG AGGACCACTG CAGCATTGCC 
CAGCAGAGAA ACATGGCCCA ATACTTCAAG AAGGTGCTGG GGGACACACT CCTCACCAAG 
CCCGTGGAGA TCTCTGCCGA CGGGCTCCCC TCACCCAACC AGCTTAAGAG GAAGATCCTC 
ATCAAGCACA AGAAGCTGGC TGAGGGCAGT GCCTACGAGG AGGTGCCTAC ATCCATGATG 
TACTCTGAGA ACGACATCAG CAACTCTATC AAGAATGGCA TCCTCTACCT GGAGGACCCT 
GTGAACCACG AATGGTATCC CCACTACTTT GTTCTGACCA GCAGCAAGAT CTACTACTCT 
GAGGAGACCA GCAGTGACCA GGGCAACGAG GATGAGGAGG AGCCCAAGGA GGTCAGCAGC 
AGCACAGAGC TGCACTCCAA TGAGAAGTGG TTCCATGGGA AGCTAGGGGC AGGGCGTGAC 
GGGCGTCACA TCGCTGAGCG CCTGCTTACT GAGTACTGCA TCGAGACCGG AGCCCCTGAC 
GGCTCCTTCC TCGTGCGAGA GAGTGAGACC TTCGTGGGCG ACTACACGCT CTCTTTCTGG 
CGGAACGGGA AAGTCCAGCA CTGCCGTATC CACTCCCGGC AAGATGCTGG GACCCCCAAG 
TTCTTCTTGA CAGACAACCT CGTCTTTGAC TCCCTCTATG ACCTCATCAC GCACTACCAG 
CAGGTGCCCC TGCGCTGTAA TGAGTTTGAG ATGCGACTTT CAGAGCCTGT CCCACAGACC 
AACGCCCACG AGAGCAAAGA GTGGTACCAC GCGAGCCTGA CCAGAGCACA GGCTGAGCAC 
ATGCTAATGC GCGTCCCTCG TGATGGGGCC TTCCTGGTGC GGAAGCGGAA TGAACCCAAC 
TCATATGCCA TCTCTTTCCG GGCTGAGGGC AAGATCAAGC ATTGCCGTGT CCAGCAAGAG 
GGCCAGACAG TGATGCTAGG GAACTCGGAG TTCGACAGCC TTGTTGACCT CATCAGCTAC 
TATGAGAAAC ACCCGCTATA CCGCAAGATG AAGCTGCGCT ATCCCATCAA CGAGGAGGCA 
CTGGAGAAGA TTGGCACAGC TGAGCCTGAC TACGGGGCCC TGTATGAGGG ACGCAACCCT 
GGCTTCTATG TAGAGGCAAA CCCTATGCCA ACTTTCAAGT GTGCAGTCAA AGCCCTCTTT 
GACTACAAGG CCCAGAGGGA GGACGAGCTG ACCTTCATCA AGAGCGCCAT CATCCAGAAT 
GTGGAGAAGC AAGAGGGAGG CTGGTGGCGA GGGGACTACG GAGGGAAGAA GCAGCTGTGG 
TTCCCATCAA ACTACGTGGA AGAGATGGTC AACCCCGTGG CCCTGGAGCC GGAGAGGGAG 
CACTTGGACG AGAACAGCCC CCTAGGGGAC TTGCTGCGGG GGGTCTTGGA TGTGCCGGCT 
TGTCAGATTG CATGGCGTCG GTGGCCCACT GGTCCCTGGA TGTTGCTGCC GACTCACAGG 
AGGAGCTGCA GGACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interplay between FGFR2b-induced autophagy and phagocytosis: role of PLC?-mediated signalling.
Journal of cellular and molecular medicine22(1)668-683(2018 Jan)
Nanni M,Ranieri D,Raffa S,Torrisi MR,Belleudi F

Mutant FGFR3 associated with SADDAN disease causes cytoskeleton disorganization through PLC?1/Src-mediated paxillin hyperphosphorylation.
The international journal of biochemistry & cell biology9517-26(2017 Dec)
Montone R,Romanelli MG,Baruzzi A,Ferrarini F,Liboi E,Lievens PM

PLC?1: Potential arbitrator of cancer progression.
Advances in biological regulation(2017 Nov)
Jang HJ,Suh PG,Lee YJ,Shin KJ,Cocco L,Chae YC

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PLCG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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