PLCB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCB2
Entrez Gene ID	5330
Full Name	phospholipase C beta 2
Synonyms	PLC-beta-2
General protein information	Preferred Names phospholipase C beta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 phosphoinositide phospholipase C-beta-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017].
Disorder MIM:	604114
Disorder Html:	Platelet PLC beta-2 deficiency (1)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017022318.1	XP_016877807.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X6
XM_011521674.2	XP_011519976.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X5
XM_017022319.2	XP_016877808.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X8
NM_001284299.2	NP_001271228.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 4
NM_001284297.2	NP_001271226.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 2
XM_017022315.1	XP_016877804.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X2
NM_001284299.1	NP_001271228.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 4
XM_017022314.2	XP_016877803.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X3
XM_024449951.1	XP_024305719.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X6
NM_001284297.1	NP_001271226.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 2
NM_001284298.1	NP_001271227.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 3
XM_024449948.1	XP_024305716.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X1
XM_024449952.1	XP_024305720.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X7
NM_001284298.2	NP_001271227.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 3
NM_004573.2	NP_004564.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 1
XM_017022319.1	XP_016877808.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X7
XM_024449950.1	XP_024305718.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X4
NM_004573.3	NP_004564.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 1
XM_017022314.1	XP_016877803.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X1
XM_017022316.1	XP_016877805.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X3
XM_017022317.1	XP_016877806.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X4
XM_024449949.1	XP_024305717.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X2
XM_017022317.2	XP_016877806.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCB2	XP_510305.4
Rattus norvegicus (Norway rat)	Plcb2	NP_445930.1
Homo sapiens (human)	PLCB2	NP_001271227.1
Canis lupus familiaris (dog)	PLCB2	XP_003640081.1
Xenopus tropicalis (tropical clawed frog)	plcb2	XP_004917351.1
Mus musculus (house mouse)	Plcb2	NP_808236.2
	PLCB2	XP_001095135.2
Bos taurus (cattle)	PLCB2	NP_001178330.1
Gallus gallus (chicken)	PLCB2	XP_420945.3

Gene Ontology
Bibliography

Related articles in PubMed

Up-modulation of PLC-?2 reduces the number and malignancy of triple-negative breast tumor cells with a CD133
Brugnoli F, Grassilli S, Lanuti P, Marchisio M, Al-Qassab Y, Vezzali F, Capitani S, Bertagnolo V
BMC cancer17(1)617(2017 Sep)

PLC-?2 is modulated by low oxygen availability in breast tumor cells and plays a phenotype dependent role in their hypoxia-related malignant potential.
Brugnoli F, Grassilli S, Al-Qassab Y, Capitani S, Bertagnolo V
Molecular carcinogenesis55(12)2210-2221(2016 Dec)

Nuclear factor-?B regulates expression of platelet phospholipase C-?2 (PLCB2).
Mao G, Jin J, Kunapuli SP, Rao AK
Thrombosis and haemostasis116(5)931-940(2016 Oct)

Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.
Block H, Stadtmann A, Riad D, Rossaint J, Sohlbach C, Germena G, Wu D, Simon SI, Ley K, Zarbock A
Blood127(3)314-24(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypoxia modulates the expression of PLC-beta-2 in breast tumor cells in a phenotype-related manner, since a decrease of the protein was observed in the BT-474 and MCF7 cell lines while an increase was revealed in MDA-MB-231 cells as a consequence of low oxygen availability.
Title: PLC-?2 is modulated by low oxygen availability in breast tumor cells and plays a phenotype dependent role in their hypoxia-related malignant potential.

Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 activation and neutrophil arrest in vivo
Title: Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.

Neuropeptide Y decreased PLCB2 expression in HUVECs.
Title: Neuropeptide Y reduces the expression of PLCB2, PLCD1 and selected PLC genes in cultured human endothelial cells.

high expression of CD133 in triple-negative breast cancer (TNBC)-derived cells correlates with high invasive potential; aggressive properties of CD133high cells are mitigated by PLC-beta2 which down-modulates expression of CD133 and may play a role in preventing metastatic progression of CD133 positive TNBC
Title: In triple negative breast tumor cells, PLC-?2 promotes the conversion of CD133high to CD133low phenotype and reduces the CD133-related invasiveness.

In this study, we show that staphylococcal enterotoxin B activates a Galphaq and PLCbeta2-dependent pathway in human T cells
Title: Superantigen-induced steroid resistance depends on activation of phospholipase C?2.

The following PLCB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30364	NM_004573.3 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$594.50-$832.30 $1189.00
OHu10415	NM_001284299.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu104026	XM_024449948.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104027	XM_024449949.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104028	XM_024449950.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104029	XM_024449951.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104030	XM_024449952.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu00641	NM_001284298.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu05315	NM_001284297.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu59756	XM_011521674.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85494	XM_017022314.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85495	XM_017022315.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85496	XM_017022316.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85497	XM_017022317.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85498	XM_017022318.1 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85499	XM_017022319.2 Latest version!	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OHu10415	NM_001284299.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu05315	NM_001284297.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu00641	NM_001284298.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85499	XM_017022319.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu30364	NM_004573.2	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$594.50-$832.30 $1189.00
OHu85494	XM_017022314.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu85497	XM_017022317.1	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCB2 ( NM_004573.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004564.2 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30364
Clone ID Related Accession (Same CDS sequence)	NM_004573.2 , NM_004573.3
Accession Version	NM_004573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, AB209583.1, M95678.1 and BU147302.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2006 this sequence version replaced NM_004573.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291657.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004564.2
CDS	302..3859
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_004573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004573.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_004573.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004564.2 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.

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CloneID	OHu30364
Clone ID Related Accession (Same CDS sequence)	NM_004573.2 , NM_004573.3
Accession Version	NM_004573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, AB209583.1, M95678.1 and BU147302.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004573.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291657.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260402.8/ ENSP00000260402.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004564.2
CDS	264..3821
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_004573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004573.3

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284299.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271228.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10415
Clone ID Related Accession (Same CDS sequence)	NM_001284299.2 , NM_001284299.1
Accession Version	NM_001284299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, BC009009.1 and AC020658.6. On May 31, 2019 this sequence version replaced NM_001284299.1. Transcript Variant: This variant (4) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009009.1, CV029736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AACCTGGAGG AGCGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271228.1
CDS	264..863
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKPGGAR

Target ORF information:

RefSeq Version	NM_001284299.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284299.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AACCTGGAGG AGCGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284299.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271228.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10415
Clone ID Related Accession (Same CDS sequence)	NM_001284299.2 , NM_001284299.1
Accession Version	NM_001284299.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, BC009009.1 and AC020658.6. Transcript Variant: This variant (4) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009009.1, BC000939.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AACCTGGAGG AGCGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271228.1
CDS	302..901
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKPGGAR

Target ORF information:

RefSeq Version	NM_001284299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284299.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AACCTGGAGG AGCGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_024449948.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305716.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104026
Clone ID Related Accession (Same CDS sequence)	XM_024449948.1
Accession Version	XM_024449948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AAGTACGACT GCCTCCCTCC CACACCTGCT 
TCGCCTCCCA GCAAGGGCTC AGCCGCACAA AACAGCTCCT TCATGCCTGT CTCTCTGCAG 
CGACACCAAA GAGATGAAGA AAAAGCTGGA GACAAAGAGA CTGGAGCGGA TCCAGGGCAT 
GACCAAAGTC ACCACAGACA AGATGGCCCA GGAGAGATGA CGGAGAACTT GGAGAGGCAC 
CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT TGCCTGGAAC AGATACGGGA GATGGAAAAG 
CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG 
GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG ACCTGCTTTC CCTCCGAGGC CAAGGACAAG 
CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG CTGTGTGAGC AGGACCCACT CATAGCAAAG 
GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305716.1
CDS	274..3900
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSEKYDCLPPTPASPPSKGSAAQNSSFMPVSLQRHQRDEEKAGDKETGADPGHDQSHHRQDGPGEMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_024449948.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449948.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AAGTACGACT GCCTCCCTCC CACACCTGCT 
TCGCCTCCCA GCAAGGGCTC AGCCGCACAA AACAGCTCCT TCATGCCTGT CTCTCTGCAG 
CGACACCAAA GAGATGAAGA AAAAGCTGGA GACAAAGAGA CTGGAGCGGA TCCAGGGCAT 
GACCAAAGTC ACCACAGACA AGATGGCCCA GGAGAGATGA CGGAGAACTT GGAGAGGCAC 
CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT TGCCTGGAAC AGATACGGGA GATGGAAAAG 
CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG 
GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG ACCTGCTTTC CCTCCGAGGC CAAGGACAAG 
CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG CTGTGTGAGC AGGACCCACT CATAGCAAAG 
GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_024449949.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305717.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104027
Clone ID Related Accession (Same CDS sequence)	XM_024449949.1
Accession Version	XM_024449949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCCAGGGCCG GGGCCAGGGA AGAGGCTATG AAAGAAGCTG CGGAGCCGCG GACCGCCAGC 
CTGGAGGAGC TCCGGGAGCT AAAGGGCGTG GTGAAGCTGC AGCGGCGGCA CGAGAAGGAG 
CTGCGAGAGT TGGAGCGGCG CGGAGCGCGG CGCTGGGAGG AGCTGCTGCA GCGGGGCGCG 
GCGCAGCTGG CGGAGCTCGG GCCACCGGGC GTGGGGGGCG TCGGGGCCTG CAAGCTCGGT 
CCCGGCAAGG GCTCTCGCAA GAAGAGGAGC CTGCCCCGCG AGGAGAGCGC CGGAGCCGCG 
CCGGGCGAGG GCCCTGAGGG CGTGGACGGG CGCGTGCGGG AGCTGAAAGA CAGGCTGGAG 
CTGGAGCTGC TGCGGCAGGG CGAGGAGCAG TACGAGTGCG TTCTGAAGCG CAAGGAGCAG 
CACGTGGCCG AGCAAATCTC CAAAATGATG GAGCTGGCCA GAGAGAAACA GGCGGCAGAG 
CTGAAGGCCC TGAAGGAGAC GTCGGAGAAG TACGACTGCC TCCCTCCCAC ACCTGCTTCG 
CCTCCCAGCA AGGGCTCAGC CGCACAAAAC AGCTCCTTCA TGCCTGTCTC TCTGCAGCGA 
CACCAAAGAG ATGAAGAAAA AGCTGGAGAC AAAGAGACTG GAGCGGATCC AGGGCATGAC 
CAAAGTCACC ACAGACAAGA TGGCCCAGGA GAGATGACGG AGAACTTGGA GAGGCACCAG 
GAGAAGCTGG AGGAGAAGCA GGCGGCTTGC CTGGAACAGA TACGGGAGAT GGAAAAGCAG 
TTCCAGAAGG AGGCGCTGGC AGAGTACGAG GCCAGGATGA AGGGTCTGGA GGCAGAGGTG 
AAGGAGTCGG TGAGGGCCTG CCTCAGGACC TGCTTTCCCT CCGAGGCCAA GGACAAGCCT 
GAGAGGGCCT GCGAGTGCCC CCCAGAGCTG TGTGAGCAGG ACCCACTCAT AGCAAAGGCA 
GATGCCCAGG AGAGCCGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305717.1
CDS	274..3897
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSEKYDCLPPTPASPPSKGSAAQNSSFMPVSLQRHQRDEEKAGDKETGADPGHDQSHHRQDGPGEMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_024449949.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449949.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCCAGGGCCG GGGCCAGGGA AGAGGCTATG AAAGAAGCTG CGGAGCCGCG GACCGCCAGC 
CTGGAGGAGC TCCGGGAGCT AAAGGGCGTG GTGAAGCTGC AGCGGCGGCA CGAGAAGGAG 
CTGCGAGAGT TGGAGCGGCG CGGAGCGCGG CGCTGGGAGG AGCTGCTGCA GCGGGGCGCG 
GCGCAGCTGG CGGAGCTCGG GCCACCGGGC GTGGGGGGCG TCGGGGCCTG CAAGCTCGGT 
CCCGGCAAGG GCTCTCGCAA GAAGAGGAGC CTGCCCCGCG AGGAGAGCGC CGGAGCCGCG 
CCGGGCGAGG GCCCTGAGGG CGTGGACGGG CGCGTGCGGG AGCTGAAAGA CAGGCTGGAG 
CTGGAGCTGC TGCGGCAGGG CGAGGAGCAG TACGAGTGCG TTCTGAAGCG CAAGGAGCAG 
CACGTGGCCG AGCAAATCTC CAAAATGATG GAGCTGGCCA GAGAGAAACA GGCGGCAGAG 
CTGAAGGCCC TGAAGGAGAC GTCGGAGAAG TACGACTGCC TCCCTCCCAC ACCTGCTTCG 
CCTCCCAGCA AGGGCTCAGC CGCACAAAAC AGCTCCTTCA TGCCTGTCTC TCTGCAGCGA 
CACCAAAGAG ATGAAGAAAA AGCTGGAGAC AAAGAGACTG GAGCGGATCC AGGGCATGAC 
CAAAGTCACC ACAGACAAGA TGGCCCAGGA GAGATGACGG AGAACTTGGA GAGGCACCAG 
GAGAAGCTGG AGGAGAAGCA GGCGGCTTGC CTGGAACAGA TACGGGAGAT GGAAAAGCAG 
TTCCAGAAGG AGGCGCTGGC AGAGTACGAG GCCAGGATGA AGGGTCTGGA GGCAGAGGTG 
AAGGAGTCGG TGAGGGCCTG CCTCAGGACC TGCTTTCCCT CCGAGGCCAA GGACAAGCCT 
GAGAGGGCCT GCGAGTGCCC CCCAGAGCTG TGTGAGCAGG ACCCACTCAT AGCAAAGGCA 
GATGCCCAGG AGAGCCGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_024449950.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305718.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.

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CloneID	OHu104028
Clone ID Related Accession (Same CDS sequence)	XM_024449950.1
Accession Version	XM_024449950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCCCCGGC TGTTTACCTG TATCTTCTCT GGACACAAGC ACAAAAAGAA CCGAAGTTAT 
GTCATCTCGT CCTTCACAGA GCTCAAGGCA TATGACCTGC TCTCCAAGGC CTCGGTGCAG 
TTTGTGGACT ACAACAAGCG CCAGATGAGC CGCATTTACC CCAAGGGAAC CCGCATGGAC 
TCCTCCAACT ACATGCCCCA GATGTTCTGG AATGCTGGAT GCCAGATGGT TGCCCTCAAC 
TTCCAGACGA TGGACTTGCC CATGCAGCAG AACATGGCAG TATTTGAGTT CAACGGGCAG 
AGCGGCTACC TCCTCAAGCA TGAGTTCATG CGCCGGCCGG ACAAGCAGTT CAACCCCTTC 
TCAGTGGACC GCATCGACGT GGTGGTGGCC ACCACCCTTT CCATTACGGT GATCTCTGGG 
CAGTTCCTGT CAGAACGCAG CGTGCGCACC TATGTAGAAG TGGAGCTGTT TGGCCTTCCT 
GGGGACCCCA AGAGGCGCTA TCGAACTAAG CTGTCACCCA GTACTAACTC CATCAATCCT 
GTCTGGAAGG AGGAGCCCTT TGTCTTTGAG AAGATCTTGA TGCCTGAGCT GGCCTCCCTC 
AGAGTGGCTG TGATGGAGGA AGGCAACAAG TTTCTTGGAC ACCGCATCAT CCCCATCAAT 
GCCCTAAATT CTGGGTACCA CCACCTGTGC CTGCACAGTG AGAGCAACAT GCCCCTCACC 
ATGCCTGCGC TCTTCATCTT CCTGGAGATG AAGGACTACA TACCTGGTGC TTGGGCAGAT 
CTCACTGTGG CCCTCGCCAA CCCCATTAAG TTCTTCAGTG CCCATGACAC GAAGTCTGTG 
AAGCTCAAGG AGGCCATGGG AGGTCTGCCT GAGAAGCCCT TCCCACTGGC GAGTCCAGTT 
GCCAGCCAGG TCAATGGGGC GTTGGCCCCA ACGAGCAATG GGTCACCAGC AGCCAGGGCC 
GGGGCCAGGG AAGAGGCTAT GAAAGAAGCT GCGGAGCCGC GGACCGCCAG CCTGGAGGAG 
CTCCGGGAGC TAAAGGGCGT GGTGAAGCTG CAGCGGCGGC ACGAGAAGGA GCTGCGAGAG 
TTGGAGCGGC GCGGAGCGCG GCGCTGGGAG GAGCTGCTGC AGCGGGGCGC GGCGCAGCTG 
GCGGAGCTCG GGCCACCGGG CGTGGGGGGC GTCGGGGCCT GCAAGCTCGG TCCCGGCAAG 
GGCTCTCGCA AGAAGAGGAG CCTGCCCCGC GAGGAGAGCG CCGGAGCCGC GCCGGGCGAG 
GGCCCTGAGG GCGTGGACGG GCGCGTGCGG GAGCTGAAAG ACAGGCTGGA GCTGGAGCTG 
CTGCGGCAGG GCGAGGAGCA GTACGAGTGC GTTCTGAAGC GCAAGGAGCA GCACGTGGCC 
GAGCAAATCT CCAAAATGAT GGAGCTGGCC AGAGAGAAAC AGGCGGCAGA GCTGAAGGCC 
CTGAAGGAGA CGTCGGAGAA GTACGACTGC CTCCCTCCCA CACCTGCTTC GCCTCCCAGC 
AAGGGCTCAG CCGCACAAAA CAGCTCCTTC ATGCCTGTCT CTCTGCAGCG ACACCAAAGA 
GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC CAGGGCATGA CCAAAGTCAC 
CACAGACAAG ATGGCCCAGG AGAGATGACG GAGAACTTGG AGAGGCACCA GGAGAAGCTG 
GAGGAGAAGC AGGCGGCTTG CCTGGAACAG ATACGGGAGA TGGAAAAGCA GTTCCAGAAG 
GAGGCGCTGG CAGAGTACGA GGCCAGGATG AAGGGTCTGG AGGCAGAGGT GAAGGAGTCG 
GTGAGGGCCT GCCTCAGGAC CTGCTTTCCC TCCGAGGCCA AGGACAAGCC TGAGAGGGCC 
TGCGAGTGCC CCCCAGAGCT GTGTGAGCAG GACCCACTCA TAGCAAAGGC AGATGCCCAG 
GAGAGCCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305718.1
CDS	274..3888
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSEKYDCLPPTPASPPSKGSAAQNSSFMPVSLQRHQRDEEKAGDKETGADPGHDQSHHRQDGPGEMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_024449950.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449950.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCCCCGGC TGTTTACCTG TATCTTCTCT GGACACAAGC ACAAAAAGAA CCGAAGTTAT 
GTCATCTCGT CCTTCACAGA GCTCAAGGCA TATGACCTGC TCTCCAAGGC CTCGGTGCAG 
TTTGTGGACT ACAACAAGCG CCAGATGAGC CGCATTTACC CCAAGGGAAC CCGCATGGAC 
TCCTCCAACT ACATGCCCCA GATGTTCTGG AATGCTGGAT GCCAGATGGT TGCCCTCAAC 
TTCCAGACGA TGGACTTGCC CATGCAGCAG AACATGGCAG TATTTGAGTT CAACGGGCAG 
AGCGGCTACC TCCTCAAGCA TGAGTTCATG CGCCGGCCGG ACAAGCAGTT CAACCCCTTC 
TCAGTGGACC GCATCGACGT GGTGGTGGCC ACCACCCTTT CCATTACGGT GATCTCTGGG 
CAGTTCCTGT CAGAACGCAG CGTGCGCACC TATGTAGAAG TGGAGCTGTT TGGCCTTCCT 
GGGGACCCCA AGAGGCGCTA TCGAACTAAG CTGTCACCCA GTACTAACTC CATCAATCCT 
GTCTGGAAGG AGGAGCCCTT TGTCTTTGAG AAGATCTTGA TGCCTGAGCT GGCCTCCCTC 
AGAGTGGCTG TGATGGAGGA AGGCAACAAG TTTCTTGGAC ACCGCATCAT CCCCATCAAT 
GCCCTAAATT CTGGGTACCA CCACCTGTGC CTGCACAGTG AGAGCAACAT GCCCCTCACC 
ATGCCTGCGC TCTTCATCTT CCTGGAGATG AAGGACTACA TACCTGGTGC TTGGGCAGAT 
CTCACTGTGG CCCTCGCCAA CCCCATTAAG TTCTTCAGTG CCCATGACAC GAAGTCTGTG 
AAGCTCAAGG AGGCCATGGG AGGTCTGCCT GAGAAGCCCT TCCCACTGGC GAGTCCAGTT 
GCCAGCCAGG TCAATGGGGC GTTGGCCCCA ACGAGCAATG GGTCACCAGC AGCCAGGGCC 
GGGGCCAGGG AAGAGGCTAT GAAAGAAGCT GCGGAGCCGC GGACCGCCAG CCTGGAGGAG 
CTCCGGGAGC TAAAGGGCGT GGTGAAGCTG CAGCGGCGGC ACGAGAAGGA GCTGCGAGAG 
TTGGAGCGGC GCGGAGCGCG GCGCTGGGAG GAGCTGCTGC AGCGGGGCGC GGCGCAGCTG 
GCGGAGCTCG GGCCACCGGG CGTGGGGGGC GTCGGGGCCT GCAAGCTCGG TCCCGGCAAG 
GGCTCTCGCA AGAAGAGGAG CCTGCCCCGC GAGGAGAGCG CCGGAGCCGC GCCGGGCGAG 
GGCCCTGAGG GCGTGGACGG GCGCGTGCGG GAGCTGAAAG ACAGGCTGGA GCTGGAGCTG 
CTGCGGCAGG GCGAGGAGCA GTACGAGTGC GTTCTGAAGC GCAAGGAGCA GCACGTGGCC 
GAGCAAATCT CCAAAATGAT GGAGCTGGCC AGAGAGAAAC AGGCGGCAGA GCTGAAGGCC 
CTGAAGGAGA CGTCGGAGAA GTACGACTGC CTCCCTCCCA CACCTGCTTC GCCTCCCAGC 
AAGGGCTCAG CCGCACAAAA CAGCTCCTTC ATGCCTGTCT CTCTGCAGCG ACACCAAAGA 
GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC CAGGGCATGA CCAAAGTCAC 
CACAGACAAG ATGGCCCAGG AGAGATGACG GAGAACTTGG AGAGGCACCA GGAGAAGCTG 
GAGGAGAAGC AGGCGGCTTG CCTGGAACAG ATACGGGAGA TGGAAAAGCA GTTCCAGAAG 
GAGGCGCTGG CAGAGTACGA GGCCAGGATG AAGGGTCTGG AGGCAGAGGT GAAGGAGTCG 
GTGAGGGCCT GCCTCAGGAC CTGCTTTCCC TCCGAGGCCA AGGACAAGCC TGAGAGGGCC 
TGCGAGTGCC CCCCAGAGCT GTGTGAGCAG GACCCACTCA TAGCAAAGGC AGATGCCCAG 
GAGAGCCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_024449951.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305719.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu104029
Clone ID Related Accession (Same CDS sequence)	XM_024449951.1
Accession Version	XM_024449951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAAGTACGAC 
TGCCTCCCTC CCACACCTGC TTCGCCTCCC AGCAAGGGCT CAGCCGCACA AAACAGCTCC 
TTCATGCCTG TCTCTCTGCA GCGACACCAA AGAGATGAAG AAAAAGCTGG AGACAAAGAG 
ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC AAGATGGCCC AGGAGAGATG 
ACGGAGAACT TGGAGAGGCA CCAGGAGAAG CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA 
CAGATACGGG AGATGGAAAA GCAGTTCCAG AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG 
ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG TCGGTGAGGG CCTGCCTCAG GACCTGCTTT 
CCCTCCGAGG CCAAGGACAA GCCTGAGAGG GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG 
CAGGACCCAC TCATAGCAAA GGCAGATGCC CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305719.1
CDS	274..3861
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSEKYDCLPPTPASPPSKGSAAQNSSFMPVSLQRHQRDEEKAGDKETGADPGHDQSHHRQDGPGEMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_024449951.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449951.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAAGTACGAC 
TGCCTCCCTC CCACACCTGC TTCGCCTCCC AGCAAGGGCT CAGCCGCACA AAACAGCTCC 
TTCATGCCTG TCTCTCTGCA GCGACACCAA AGAGATGAAG AAAAAGCTGG AGACAAAGAG 
ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC AAGATGGCCC AGGAGAGATG 
ACGGAGAACT TGGAGAGGCA CCAGGAGAAG CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA 
CAGATACGGG AGATGGAAAA GCAGTTCCAG AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG 
ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG TCGGTGAGGG CCTGCCTCAG GACCTGCTTT 
CCCTCCGAGG CCAAGGACAA GCCTGAGAGG GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG 
CAGGACCCAC TCATAGCAAA GGCAGATGCC CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_024449952.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305720.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu104030
Clone ID Related Accession (Same CDS sequence)	XM_024449952.1
Accession Version	XM_024449952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GAGCCGCGGA CCGCCAGCCT GGAGGAGCTC CGGGAGCTAA AGGGCGTGGT GAAGCTGCAG 
CGGCGGCACG AGAAGGAGCT GCGAGAGTTG GAGCGGCGCG GAGCGCGGCG CTGGGAGGAG 
CTGCTGCAGC GGGGCGCGGC GCAGCTGGCG GAGCTCGGGC CACCGGGCGT GGGGGGCGTC 
GGGGCCTGCA AGCTCGGTCC CGGCAAGGGC TCTCGCAAGA AGAGGAGCCT GCCCCGCGAG 
GAGAGCGCCG GAGCCGCGCC GGGCGAGGGC CCTGAGGGCG TGGACGGGCG CGTGCGGGAG 
CTGAAAGACA GGCTGGAGCT GGAGCTGCTG CGGCAGGGCG AGGAGCAGTA CGAGTGCGTT 
CTGAAGCGCA AGGAGCAGCA CGTGGCCGAG CAAATCTCCA AAATGATGGA GCTGGCCAGA 
GAGAAACAGG CGGCAGAGCT GAAGGCCCTG AAGGAGACGT CGGAGAAGTA CGACTGCCTC 
CCTCCCACAC CTGCTTCGCC TCCCAGCAAG GGCTCAGCCG CACAAAACAG CTCCTTCATG 
CCTGTCTCTC TGCAGCGACA CCAAAGAGAT GAAGAAAAAG CTGGAGACAA AGAGACTGGA 
GCGGATCCAG GGCATGACCA AAGTCACCAC AGACAAGATG GCCCAGGAGA GATGACGGAG 
AACTTGGAGA GGCACCAGGA GAAGCTGGAG GAGAAGCAGG CGGCTTGCCT GGAACAGATA 
CGGGAGATGG AAAAGCAGTT CCAGAAGGAG GCGCTGGCAG AGTACGAGGC CAGGATGAAG 
GGTCTGGAGG CAGAGGTGAA GGAGTCGGTG AGGGCCTGCC TCAGGACCTG CTTTCCCTCC 
GAGGCCAAGG ACAAGCCTGA GAGGGCCTGC GAGTGCCCCC CAGAGCTGTG TGAGCAGGAC 
CCACTCATAG CAAAGGCAGA TGCCCAGGAG AGCCGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305720.1
CDS	274..3855
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSEKYDCLPPTPASPPSKGSAAQNSSFMPVSLQRHQRDEEKAGDKETGADPGHDQSHHRQDGPGEMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_024449952.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449952.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GAGCCGCGGA CCGCCAGCCT GGAGGAGCTC CGGGAGCTAA AGGGCGTGGT GAAGCTGCAG 
CGGCGGCACG AGAAGGAGCT GCGAGAGTTG GAGCGGCGCG GAGCGCGGCG CTGGGAGGAG 
CTGCTGCAGC GGGGCGCGGC GCAGCTGGCG GAGCTCGGGC CACCGGGCGT GGGGGGCGTC 
GGGGCCTGCA AGCTCGGTCC CGGCAAGGGC TCTCGCAAGA AGAGGAGCCT GCCCCGCGAG 
GAGAGCGCCG GAGCCGCGCC GGGCGAGGGC CCTGAGGGCG TGGACGGGCG CGTGCGGGAG 
CTGAAAGACA GGCTGGAGCT GGAGCTGCTG CGGCAGGGCG AGGAGCAGTA CGAGTGCGTT 
CTGAAGCGCA AGGAGCAGCA CGTGGCCGAG CAAATCTCCA AAATGATGGA GCTGGCCAGA 
GAGAAACAGG CGGCAGAGCT GAAGGCCCTG AAGGAGACGT CGGAGAAGTA CGACTGCCTC 
CCTCCCACAC CTGCTTCGCC TCCCAGCAAG GGCTCAGCCG CACAAAACAG CTCCTTCATG 
CCTGTCTCTC TGCAGCGACA CCAAAGAGAT GAAGAAAAAG CTGGAGACAA AGAGACTGGA 
GCGGATCCAG GGCATGACCA AAGTCACCAC AGACAAGATG GCCCAGGAGA GATGACGGAG 
AACTTGGAGA GGCACCAGGA GAAGCTGGAG GAGAAGCAGG CGGCTTGCCT GGAACAGATA 
CGGGAGATGG AAAAGCAGTT CCAGAAGGAG GCGCTGGCAG AGTACGAGGC CAGGATGAAG 
GGTCTGGAGG CAGAGGTGAA GGAGTCGGTG AGGGCCTGCC TCAGGACCTG CTTTCCCTCC 
GAGGCCAAGG ACAAGCCTGA GAGGGCCTGC GAGTGCCCCC CAGAGCTGTG TGAGCAGGAC 
CCACTCATAG CAAAGGCAGA TGCCCAGGAG AGCCGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284298.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271227.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.

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CloneID	OHu00641
Clone ID Related Accession (Same CDS sequence)	NM_001284298.1 , NM_001284298.2
Accession Version	NM_001284298.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, BC136467.1, AB209583.1 and BU147302.1. On Sep 27, 2013 this sequence version replaced XM_005254447.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGAGCCGCGG ACCGCCAGCC TGGAGGAGCT CCGGGAGCTA 
AAGGGCGTGG TGAAGCTGCA GCGGCGGCAC GAGAAGGAGC TGCGAGAGTT GGAGCGGCGC 
GGAGCGCGGC GCTGGGAGGA GCTGCTGCAG CGGGGCGCGG CGCAGCTGGC GGAGCTCGGG 
CCACCGGGCG TGGGGGGCGT CGGGGCCTGC AAGCTCGGTC CCGGCAAGGG CTCTCGCAAG 
AAGAGGAGCC TGCCCCGCGA GGAGAGCGCC GGAGCCGCGC CGGGCGAGGG CCCTGAGGGC 
GTGGACGGGC GCGTGCGGGA GCTGAAAGAC AGGCTGGAGC TGGAGCTGCT GCGGCAGGGC 
GAGGAGCAGT ACGAGTGCGT TCTGAAGCGC AAGGAGCAGC ACGTGGCCGA GCAAATCTCC 
AAAATGATGG AGCTGGCCAG AGAGAAACAG GCGGCAGAGC TGAAGGCCCT GAAGGAGACG 
TCGGAGAACG ACACCAAAGA GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC 
CAGGGCATGA CCAAAGTCAC CACAGACAAG ATGGCCCAGG AGAGGTTGAA GAGAGAGATT 
AACAACTCCC ACATCCAGGA AGTAGTGCAG GTGATCAAGC AGATGACGGA GAACTTGGAG 
AGGCACCAGG AGAAGCTGGA GGAGAAGCAG GCGGCTTGCC TGGAACAGAT ACGGGAGATG 
GAAAAGCAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271227.1
CDS	302..3814
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_001284298.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284298.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGAGCCGCGG ACCGCCAGCC TGGAGGAGCT CCGGGAGCTA 
AAGGGCGTGG TGAAGCTGCA GCGGCGGCAC GAGAAGGAGC TGCGAGAGTT GGAGCGGCGC 
GGAGCGCGGC GCTGGGAGGA GCTGCTGCAG CGGGGCGCGG CGCAGCTGGC GGAGCTCGGG 
CCACCGGGCG TGGGGGGCGT CGGGGCCTGC AAGCTCGGTC CCGGCAAGGG CTCTCGCAAG 
AAGAGGAGCC TGCCCCGCGA GGAGAGCGCC GGAGCCGCGC CGGGCGAGGG CCCTGAGGGC 
GTGGACGGGC GCGTGCGGGA GCTGAAAGAC AGGCTGGAGC TGGAGCTGCT GCGGCAGGGC 
GAGGAGCAGT ACGAGTGCGT TCTGAAGCGC AAGGAGCAGC ACGTGGCCGA GCAAATCTCC 
AAAATGATGG AGCTGGCCAG AGAGAAACAG GCGGCAGAGC TGAAGGCCCT GAAGGAGACG 
TCGGAGAACG ACACCAAAGA GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC 
CAGGGCATGA CCAAAGTCAC CACAGACAAG ATGGCCCAGG AGAGGTTGAA GAGAGAGATT 
AACAACTCCC ACATCCAGGA AGTAGTGCAG GTGATCAAGC AGATGACGGA GAACTTGGAG 
AGGCACCAGG AGAAGCTGGA GGAGAAGCAG GCGGCTTGCC TGGAACAGAT ACGGGAGATG 
GAAAAGCAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284298.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271227.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.

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CloneID	OHu00641
Clone ID Related Accession (Same CDS sequence)	NM_001284298.1 , NM_001284298.2
Accession Version	NM_001284298.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, BC136467.1 and AB209583.1. On May 31, 2019 this sequence version replaced NM_001284298.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGAGCCGCGG ACCGCCAGCC TGGAGGAGCT CCGGGAGCTA 
AAGGGCGTGG TGAAGCTGCA GCGGCGGCAC GAGAAGGAGC TGCGAGAGTT GGAGCGGCGC 
GGAGCGCGGC GCTGGGAGGA GCTGCTGCAG CGGGGCGCGG CGCAGCTGGC GGAGCTCGGG 
CCACCGGGCG TGGGGGGCGT CGGGGCCTGC AAGCTCGGTC CCGGCAAGGG CTCTCGCAAG 
AAGAGGAGCC TGCCCCGCGA GGAGAGCGCC GGAGCCGCGC CGGGCGAGGG CCCTGAGGGC 
GTGGACGGGC GCGTGCGGGA GCTGAAAGAC AGGCTGGAGC TGGAGCTGCT GCGGCAGGGC 
GAGGAGCAGT ACGAGTGCGT TCTGAAGCGC AAGGAGCAGC ACGTGGCCGA GCAAATCTCC 
AAAATGATGG AGCTGGCCAG AGAGAAACAG GCGGCAGAGC TGAAGGCCCT GAAGGAGACG 
TCGGAGAACG ACACCAAAGA GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC 
CAGGGCATGA CCAAAGTCAC CACAGACAAG ATGGCCCAGG AGAGGTTGAA GAGAGAGATT 
AACAACTCCC ACATCCAGGA AGTAGTGCAG GTGATCAAGC AGATGACGGA GAACTTGGAG 
AGGCACCAGG AGAAGCTGGA GGAGAAGCAG GCGGCTTGCC TGGAACAGAT ACGGGAGATG 
GAAAAGCAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271227.1
CDS	264..3776
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_001284298.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284298.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGAGCCGCGG ACCGCCAGCC TGGAGGAGCT CCGGGAGCTA 
AAGGGCGTGG TGAAGCTGCA GCGGCGGCAC GAGAAGGAGC TGCGAGAGTT GGAGCGGCGC 
GGAGCGCGGC GCTGGGAGGA GCTGCTGCAG CGGGGCGCGG CGCAGCTGGC GGAGCTCGGG 
CCACCGGGCG TGGGGGGCGT CGGGGCCTGC AAGCTCGGTC CCGGCAAGGG CTCTCGCAAG 
AAGAGGAGCC TGCCCCGCGA GGAGAGCGCC GGAGCCGCGC CGGGCGAGGG CCCTGAGGGC 
GTGGACGGGC GCGTGCGGGA GCTGAAAGAC AGGCTGGAGC TGGAGCTGCT GCGGCAGGGC 
GAGGAGCAGT ACGAGTGCGT TCTGAAGCGC AAGGAGCAGC ACGTGGCCGA GCAAATCTCC 
AAAATGATGG AGCTGGCCAG AGAGAAACAG GCGGCAGAGC TGAAGGCCCT GAAGGAGACG 
TCGGAGAACG ACACCAAAGA GATGAAGAAA AAGCTGGAGA CAAAGAGACT GGAGCGGATC 
CAGGGCATGA CCAAAGTCAC CACAGACAAG ATGGCCCAGG AGAGGTTGAA GAGAGAGATT 
AACAACTCCC ACATCCAGGA AGTAGTGCAG GTGATCAAGC AGATGACGGA GAACTTGGAG 
AGGCACCAGG AGAAGCTGGA GGAGAAGCAG GCGGCTTGCC TGGAACAGAT ACGGGAGATG 
GAAAAGCAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284297.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271226.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.

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CloneID	OHu05315
Clone ID Related Accession (Same CDS sequence)	NM_001284297.2 , NM_001284297.1
Accession Version	NM_001284297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, M95678.1 and AB209583.1. On May 31, 2019 this sequence version replaced NM_001284297.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95678.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG AGCTCAAGGC ATATGACCTG 
CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC GCCAGATGAG CCGCATTTAC 
CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC AGATGTTCTG GAATGCTGGA 
TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC CCATGCAGCA GAACATGGCA 
GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC ATGAGTTCAT GCGCCGGCCG 
GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG TGGTGGTGGC CACCACCCTT 
TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA GCGTGCGCAC CTATGTAGAA 
GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT ATCGAACTAA GCTGTCACCC 
AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT TTGTCTTTGA GAAGATCTTG 
ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG AAGGCAACAA GTTTCTTGGA 
CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC ACCACCTGTG CCTGCACAGT 
GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT TCCTGGAGAT GAAGGACTAC 
ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA ACCCCATTAA GTTCTTCAGT 
GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG GAGGTCTGCC TGAGAAGCCC 
TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG CGTTGGCCCC AACGAGCAAT 
GGGTCACCAG CAGCCAGGGC CGGGGCCAGG GAAGAGGCTA TGAAAGAAGC TGCGGAGCCG 
CGGACCGCCA GCCTGGAGGA GCTCCGGGAG CTAAAGGGCG TGGTGAAGCT GCAGCGGCGG 
CACGAGAAGG AGCTGCGAGA GTTGGAGCGG CGCGGAGCGC GGCGCTGGGA GGAGCTGCTG 
CAGCGGGGCG CGGCGCAGCT GGCGGAGCTC GGGCCACCGG GCGTGGGGGG CGTCGGGGCC 
TGCAAGCTCG GTCCCGGCAA GGGCTCTCGC AAGAAGAGGA GCCTGCCCCG CGAGGAGAGC 
GCCGGAGCCG CGCCGGGCGA GGGCCCTGAG GGCGTGGACG GGCGCGTGCG GGAGCTGAAA 
GACAGGCTGG AGCTGGAGCT GCTGCGGCAG GGCGAGGAGC AGTACGAGTG CGTTCTGAAG 
CGCAAGGAGC AGCACGTGGC CGAGCAAATC TCCAAAATGA TGGAGCTGGC CAGAGAGAAA 
CAGGCGGCAG AGCTGAAGGC CCTGAAGGAG ACGTCGGAGA ACGACACCAA AGAGATGAAG 
AAAAAGCTGG AGACAAAGAG ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC 
AAGATGGCCC AGGAGAGGTT GAAGAGAGAG ATTAACAACT CCCACATCCA GGAAGTAGTG 
CAGGTGATCA AGCAGATGAC GGAGAACTTG GAGAGGCACC AGGAGAAGCT GGAGGAGAAG 
CAGGCGGCTT GCCTGGAACA GATACGGGAG ATGGAAAAGC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271226.1
CDS	264..3809
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_001284297.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284297.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG AGCTCAAGGC ATATGACCTG 
CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC GCCAGATGAG CCGCATTTAC 
CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC AGATGTTCTG GAATGCTGGA 
TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC CCATGCAGCA GAACATGGCA 
GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC ATGAGTTCAT GCGCCGGCCG 
GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG TGGTGGTGGC CACCACCCTT 
TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA GCGTGCGCAC CTATGTAGAA 
GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT ATCGAACTAA GCTGTCACCC 
AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT TTGTCTTTGA GAAGATCTTG 
ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG AAGGCAACAA GTTTCTTGGA 
CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC ACCACCTGTG CCTGCACAGT 
GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT TCCTGGAGAT GAAGGACTAC 
ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA ACCCCATTAA GTTCTTCAGT 
GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG GAGGTCTGCC TGAGAAGCCC 
TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG CGTTGGCCCC AACGAGCAAT 
GGGTCACCAG CAGCCAGGGC CGGGGCCAGG GAAGAGGCTA TGAAAGAAGC TGCGGAGCCG 
CGGACCGCCA GCCTGGAGGA GCTCCGGGAG CTAAAGGGCG TGGTGAAGCT GCAGCGGCGG 
CACGAGAAGG AGCTGCGAGA GTTGGAGCGG CGCGGAGCGC GGCGCTGGGA GGAGCTGCTG 
CAGCGGGGCG CGGCGCAGCT GGCGGAGCTC GGGCCACCGG GCGTGGGGGG CGTCGGGGCC 
TGCAAGCTCG GTCCCGGCAA GGGCTCTCGC AAGAAGAGGA GCCTGCCCCG CGAGGAGAGC 
GCCGGAGCCG CGCCGGGCGA GGGCCCTGAG GGCGTGGACG GGCGCGTGCG GGAGCTGAAA 
GACAGGCTGG AGCTGGAGCT GCTGCGGCAG GGCGAGGAGC AGTACGAGTG CGTTCTGAAG 
CGCAAGGAGC AGCACGTGGC CGAGCAAATC TCCAAAATGA TGGAGCTGGC CAGAGAGAAA 
CAGGCGGCAG AGCTGAAGGC CCTGAAGGAG ACGTCGGAGA ACGACACCAA AGAGATGAAG 
AAAAAGCTGG AGACAAAGAG ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC 
AAGATGGCCC AGGAGAGGTT GAAGAGAGAG ATTAACAACT CCCACATCCA GGAAGTAGTG 
CAGGTGATCA AGCAGATGAC GGAGAACTTG GAGAGGCACC AGGAGAAGCT GGAGGAGAAG 
CAGGCGGCTT GCCTGGAACA GATACGGGAG ATGGAAAAGC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( NM_001284297.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271226.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.

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CloneID	OHu05315
Clone ID Related Accession (Same CDS sequence)	NM_001284297.2 , NM_001284297.1
Accession Version	NM_001284297.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA981116.1, M95678.1, AB209583.1 and BU147302.1. On Sep 27, 2013 this sequence version replaced XM_005254446.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95678.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG AGCTCAAGGC ATATGACCTG 
CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC GCCAGATGAG CCGCATTTAC 
CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC AGATGTTCTG GAATGCTGGA 
TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC CCATGCAGCA GAACATGGCA 
GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC ATGAGTTCAT GCGCCGGCCG 
GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG TGGTGGTGGC CACCACCCTT 
TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA GCGTGCGCAC CTATGTAGAA 
GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT ATCGAACTAA GCTGTCACCC 
AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT TTGTCTTTGA GAAGATCTTG 
ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG AAGGCAACAA GTTTCTTGGA 
CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC ACCACCTGTG CCTGCACAGT 
GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT TCCTGGAGAT GAAGGACTAC 
ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA ACCCCATTAA GTTCTTCAGT 
GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG GAGGTCTGCC TGAGAAGCCC 
TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG CGTTGGCCCC AACGAGCAAT 
GGGTCACCAG CAGCCAGGGC CGGGGCCAGG GAAGAGGCTA TGAAAGAAGC TGCGGAGCCG 
CGGACCGCCA GCCTGGAGGA GCTCCGGGAG CTAAAGGGCG TGGTGAAGCT GCAGCGGCGG 
CACGAGAAGG AGCTGCGAGA GTTGGAGCGG CGCGGAGCGC GGCGCTGGGA GGAGCTGCTG 
CAGCGGGGCG CGGCGCAGCT GGCGGAGCTC GGGCCACCGG GCGTGGGGGG CGTCGGGGCC 
TGCAAGCTCG GTCCCGGCAA GGGCTCTCGC AAGAAGAGGA GCCTGCCCCG CGAGGAGAGC 
GCCGGAGCCG CGCCGGGCGA GGGCCCTGAG GGCGTGGACG GGCGCGTGCG GGAGCTGAAA 
GACAGGCTGG AGCTGGAGCT GCTGCGGCAG GGCGAGGAGC AGTACGAGTG CGTTCTGAAG 
CGCAAGGAGC AGCACGTGGC CGAGCAAATC TCCAAAATGA TGGAGCTGGC CAGAGAGAAA 
CAGGCGGCAG AGCTGAAGGC CCTGAAGGAG ACGTCGGAGA ACGACACCAA AGAGATGAAG 
AAAAAGCTGG AGACAAAGAG ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC 
AAGATGGCCC AGGAGAGGTT GAAGAGAGAG ATTAACAACT CCCACATCCA GGAAGTAGTG 
CAGGTGATCA AGCAGATGAC GGAGAACTTG GAGAGGCACC AGGAGAAGCT GGAGGAGAAG 
CAGGCGGCTT GCCTGGAACA GATACGGGAG ATGGAAAAGC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271226.1
CDS	302..3847
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	NM_001284297.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284297.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG AGCTCAAGGC ATATGACCTG 
CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC GCCAGATGAG CCGCATTTAC 
CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC AGATGTTCTG GAATGCTGGA 
TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC CCATGCAGCA GAACATGGCA 
GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC ATGAGTTCAT GCGCCGGCCG 
GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG TGGTGGTGGC CACCACCCTT 
TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA GCGTGCGCAC CTATGTAGAA 
GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT ATCGAACTAA GCTGTCACCC 
AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT TTGTCTTTGA GAAGATCTTG 
ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG AAGGCAACAA GTTTCTTGGA 
CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC ACCACCTGTG CCTGCACAGT 
GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT TCCTGGAGAT GAAGGACTAC 
ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA ACCCCATTAA GTTCTTCAGT 
GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG GAGGTCTGCC TGAGAAGCCC 
TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG CGTTGGCCCC AACGAGCAAT 
GGGTCACCAG CAGCCAGGGC CGGGGCCAGG GAAGAGGCTA TGAAAGAAGC TGCGGAGCCG 
CGGACCGCCA GCCTGGAGGA GCTCCGGGAG CTAAAGGGCG TGGTGAAGCT GCAGCGGCGG 
CACGAGAAGG AGCTGCGAGA GTTGGAGCGG CGCGGAGCGC GGCGCTGGGA GGAGCTGCTG 
CAGCGGGGCG CGGCGCAGCT GGCGGAGCTC GGGCCACCGG GCGTGGGGGG CGTCGGGGCC 
TGCAAGCTCG GTCCCGGCAA GGGCTCTCGC AAGAAGAGGA GCCTGCCCCG CGAGGAGAGC 
GCCGGAGCCG CGCCGGGCGA GGGCCCTGAG GGCGTGGACG GGCGCGTGCG GGAGCTGAAA 
GACAGGCTGG AGCTGGAGCT GCTGCGGCAG GGCGAGGAGC AGTACGAGTG CGTTCTGAAG 
CGCAAGGAGC AGCACGTGGC CGAGCAAATC TCCAAAATGA TGGAGCTGGC CAGAGAGAAA 
CAGGCGGCAG AGCTGAAGGC CCTGAAGGAG ACGTCGGAGA ACGACACCAA AGAGATGAAG 
AAAAAGCTGG AGACAAAGAG ACTGGAGCGG ATCCAGGGCA TGACCAAAGT CACCACAGAC 
AAGATGGCCC AGGAGAGGTT GAAGAGAGAG ATTAACAACT CCCACATCCA GGAAGTAGTG 
CAGGTGATCA AGCAGATGAC GGAGAACTTG GAGAGGCACC AGGAGAAGCT GGAGGAGAAG 
CAGGCGGCTT GCCTGGAACA GATACGGGAG ATGGAAAAGC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_011521674.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519976.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.

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CloneID	OHu59756
Clone ID Related Accession (Same CDS sequence)	XM_011521674.2
Accession Version	XM_011521674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGGGGTCC 
TTGCTGTCTC CACAGTTCCA GAAGGAGGCG CTGGCAGAGT ACGAGGCCAG GATGAAGGGT 
CTGGAGGCAG AGGTGAAGGA GTCGGTGAGG GCCTGCCTCA GGACCTGCTT TCCCTCCGAG 
GCCAAGGACA AGCCTGAGAG GGCCTGCGAG TGCCCCCCAG AGCTGTGTGA GCAGGACCCA 
CTCATAGCAA AGGCAGATGC CCAGGAGAGC CGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519976.1
CDS	277..3855
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_011521674.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521674.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCAAAA GAACCGAAGT TATGTCATCT CGTCCTTCAC AGAGCTCAAG 
GCATATGACC TGCTCTCCAA GGCCTCGGTG CAGTTTGTGG ACTACAACAA GCGCCAGATG 
AGCCGCATTT ACCCCAAGGG AACCCGCATG GACTCCTCCA ACTACATGCC CCAGATGTTC 
TGGAATGCTG GATGCCAGAT GGTTGCCCTC AACTTCCAGA CGATGGACTT GCCCATGCAG 
CAGAACATGG CAGTATTTGA GTTCAACGGG CAGAGCGGCT ACCTCCTCAA GCATGAGTTC 
ATGCGCCGGC CGGACAAGCA GTTCAACCCC TTCTCAGTGG ACCGCATCGA CGTGGTGGTG 
GCCACCACCC TTTCCATTAC GGTGATCTCT GGGCAGTTCC TGTCAGAACG CAGCGTGCGC 
ACCTATGTAG AAGTGGAGCT GTTTGGCCTT CCTGGGGACC CCAAGAGGCG CTATCGAACT 
AAGCTGTCAC CCAGTACTAA CTCCATCAAT CCTGTCTGGA AGGAGGAGCC CTTTGTCTTT 
GAGAAGATCT TGATGCCTGA GCTGGCCTCC CTCAGAGTGG CTGTGATGGA GGAAGGCAAC 
AAGTTTCTTG GACACCGCAT CATCCCCATC AATGCCCTAA ATTCTGGGTA CCACCACCTG 
TGCCTGCACA GTGAGAGCAA CATGCCCCTC ACCATGCCTG CGCTCTTCAT CTTCCTGGAG 
ATGAAGGACT ACATACCTGG TGCTTGGGCA GATCTCACTG TGGCCCTCGC CAACCCCATT 
AAGTTCTTCA GTGCCCATGA CACGAAGTCT GTGAAGCTCA AGGAGGCCAT GGGAGGTCTG 
CCTGAGAAGC CCTTCCCACT GGCGAGTCCA GTTGCCAGCC AGGTCAATGG GGCGTTGGCC 
CCAACGAGCA ATGGGTCACC AGCAGCCAGG GCCGGGGCCA GGGAAGAGGC TATGAAAGAA 
GCTGCGGAGC CGCGGACCGC CAGCCTGGAG GAGCTCCGGG AGCTAAAGGG CGTGGTGAAG 
CTGCAGCGGC GGCACGAGAA GGAGCTGCGA GAGTTGGAGC GGCGCGGAGC GCGGCGCTGG 
GAGGAGCTGC TGCAGCGGGG CGCGGCGCAG CTGGCGGAGC TCGGGCCACC GGGCGTGGGG 
GGCGTCGGGG CCTGCAAGCT CGGTCCCGGC AAGGGCTCTC GCAAGAAGAG GAGCCTGCCC 
CGCGAGGAGA GCGCCGGAGC CGCGCCGGGC GAGGGCCCTG AGGGCGTGGA CGGGCGCGTG 
CGGGAGCTGA AAGACAGGCT GGAGCTGGAG CTGCTGCGGC AGGGCGAGGA GCAGTACGAG 
TGCGTTCTGA AGCGCAAGGA GCAGCACGTG GCCGAGCAAA TCTCCAAAAT GATGGAGCTG 
GCCAGAGAGA AACAGGCGGC AGAGCTGAAG GCCCTGAAGG AGACGTCGGA GAACGACACC 
AAAGAGATGA AGAAAAAGCT GGAGACAAAG AGACTGGAGC GGATCCAGGG CATGACCAAA 
GTCACCACAG ACAAGATGGC CCAGGAGAGG TTGAAGAGAG AGATTAACAA CTCCCACATC 
CAGGAAGTAG TGCAGGTGAT CAAGCAGATG ACGGAGAACT TGGAGAGGCA CCAGGAGAAG 
CTGGAGGAGA AGCAGGCGGC TTGCCTGGAA CAGATACGGG AGATGGAAAA GCAGGGGTCC 
TTGCTGTCTC CACAGTTCCA GAAGGAGGCG CTGGCAGAGT ACGAGGCCAG GATGAAGGGT 
CTGGAGGCAG AGGTGAAGGA GTCGGTGAGG GCCTGCCTCA GGACCTGCTT TCCCTCCGAG 
GCCAAGGACA AGCCTGAGAG GGCCTGCGAG TGCCCCCCAG AGCTGTGTGA GCAGGACCCA 
CTCATAGCAA AGGCAGATGC CCAGGAGAGC CGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022314.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877803.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.

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CloneID	OHu85494
Clone ID Related Accession (Same CDS sequence)	XM_017022314.2 , XM_017022314.1
Accession Version	XM_017022314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGGGGTCCT TGCTGTCTCC ACAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877803.1
CDS	274..3891
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022314.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022314.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGGGGTCCT TGCTGTCTCC ACAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022314.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877803.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85494
Clone ID Related Accession (Same CDS sequence)	XM_017022314.2 , XM_017022314.1
Accession Version	XM_017022314.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGGGGTCCT TGCTGTCTCC ACAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877803.1
CDS	277..3894
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022314.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022314.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGGGGTCCT TGCTGTCTCC ACAGTTCCAG 
AAGGAGGCGC TGGCAGAGTA CGAGGCCAGG ATGAAGGGTC TGGAGGCAGA GGTGAAGGAG 
TCGGTGAGGG CCTGCCTCAG GACCTGCTTT CCCTCCGAGG CCAAGGACAA GCCTGAGAGG 
GCCTGCGAGT GCCCCCCAGA GCTGTGTGAG CAGGACCCAC TCATAGCAAA GGCAGATGCC 
CAGGAGAGCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022315.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877804.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu85495
Clone ID Related Accession (Same CDS sequence)	XM_017022315.1
Accession Version	XM_017022315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCCAGGGCCG GGGCCAGGGA AGAGGCTATG AAAGAAGCTG CGGAGCCGCG GACCGCCAGC 
CTGGAGGAGC TCCGGGAGCT AAAGGGCGTG GTGAAGCTGC AGCGGCGGCA CGAGAAGGAG 
CTGCGAGAGT TGGAGCGGCG CGGAGCGCGG CGCTGGGAGG AGCTGCTGCA GCGGGGCGCG 
GCGCAGCTGG CGGAGCTCGG GCCACCGGGC GTGGGGGGCG TCGGGGCCTG CAAGCTCGGT 
CCCGGCAAGG GCTCTCGCAA GAAGAGGAGC CTGCCCCGCG AGGAGAGCGC CGGAGCCGCG 
CCGGGCGAGG GCCCTGAGGG CGTGGACGGG CGCGTGCGGG AGCTGAAAGA CAGGCTGGAG 
CTGGAGCTGC TGCGGCAGGG CGAGGAGCAG TACGAGTGCG TTCTGAAGCG CAAGGAGCAG 
CACGTGGCCG AGCAAATCTC CAAAATGATG GAGCTGGCCA GAGAGAAACA GGCGGCAGAG 
CTGAAGGCCC TGAAGGAGAC GTCGGAGAAC GACACCAAAG AGATGAAGAA AAAGCTGGAG 
ACAAAGAGAC TGGAGCGGAT CCAGGGCATG ACCAAAGTCA CCACAGACAA GATGGCCCAG 
GAGAGGTTGA AGAGAGAGAT TAACAACTCC CACATCCAGG AAGTAGTGCA GGTGATCAAG 
CAGATGACGG AGAACTTGGA GAGGCACCAG GAGAAGCTGG AGGAGAAGCA GGCGGCTTGC 
CTGGAACAGA TACGGGAGAT GGAAAAGCAG GGGTCCTTGC TGTCTCCACA GTTCCAGAAG 
GAGGCGCTGG CAGAGTACGA GGCCAGGATG AAGGGTCTGG AGGCAGAGGT GAAGGAGTCG 
GTGAGGGCCT GCCTCAGGAC CTGCTTTCCC TCCGAGGCCA AGGACAAGCC TGAGAGGGCC 
TGCGAGTGCC CCCCAGAGCT GTGTGAGCAG GACCCACTCA TAGCAAAGGC AGATGCCCAG 
GAGAGCCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877804.1
CDS	277..3891
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022315.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022315.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCCAGGGCCG GGGCCAGGGA AGAGGCTATG AAAGAAGCTG CGGAGCCGCG GACCGCCAGC 
CTGGAGGAGC TCCGGGAGCT AAAGGGCGTG GTGAAGCTGC AGCGGCGGCA CGAGAAGGAG 
CTGCGAGAGT TGGAGCGGCG CGGAGCGCGG CGCTGGGAGG AGCTGCTGCA GCGGGGCGCG 
GCGCAGCTGG CGGAGCTCGG GCCACCGGGC GTGGGGGGCG TCGGGGCCTG CAAGCTCGGT 
CCCGGCAAGG GCTCTCGCAA GAAGAGGAGC CTGCCCCGCG AGGAGAGCGC CGGAGCCGCG 
CCGGGCGAGG GCCCTGAGGG CGTGGACGGG CGCGTGCGGG AGCTGAAAGA CAGGCTGGAG 
CTGGAGCTGC TGCGGCAGGG CGAGGAGCAG TACGAGTGCG TTCTGAAGCG CAAGGAGCAG 
CACGTGGCCG AGCAAATCTC CAAAATGATG GAGCTGGCCA GAGAGAAACA GGCGGCAGAG 
CTGAAGGCCC TGAAGGAGAC GTCGGAGAAC GACACCAAAG AGATGAAGAA AAAGCTGGAG 
ACAAAGAGAC TGGAGCGGAT CCAGGGCATG ACCAAAGTCA CCACAGACAA GATGGCCCAG 
GAGAGGTTGA AGAGAGAGAT TAACAACTCC CACATCCAGG AAGTAGTGCA GGTGATCAAG 
CAGATGACGG AGAACTTGGA GAGGCACCAG GAGAAGCTGG AGGAGAAGCA GGCGGCTTGC 
CTGGAACAGA TACGGGAGAT GGAAAAGCAG GGGTCCTTGC TGTCTCCACA GTTCCAGAAG 
GAGGCGCTGG CAGAGTACGA GGCCAGGATG AAGGGTCTGG AGGCAGAGGT GAAGGAGTCG 
GTGAGGGCCT GCCTCAGGAC CTGCTTTCCC TCCGAGGCCA AGGACAAGCC TGAGAGGGCC 
TGCGAGTGCC CCCCAGAGCT GTGTGAGCAG GACCCACTCA TAGCAAAGGC AGATGCCCAG 
GAGAGCCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022316.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877805.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.

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CloneID	OHu85496
Clone ID Related Accession (Same CDS sequence)	XM_017022316.1
Accession Version	XM_017022316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCCCCGGC TGTTTACCTG TATCTTCTCT GGACACAAGC ACAAAAAGAA CCGAAGTTAT 
GTCATCTCGT CCTTCACAGA GCTCAAGGCA TATGACCTGC TCTCCAAGGC CTCGGTGCAG 
TTTGTGGACT ACAACAAGCG CCAGATGAGC CGCATTTACC CCAAGGGAAC CCGCATGGAC 
TCCTCCAACT ACATGCCCCA GATGTTCTGG AATGCTGGAT GCCAGATGGT TGCCCTCAAC 
TTCCAGACGA TGGACTTGCC CATGCAGCAG AACATGGCAG TATTTGAGTT CAACGGGCAG 
AGCGGCTACC TCCTCAAGCA TGAGTTCATG CGCCGGCCGG ACAAGCAGTT CAACCCCTTC 
TCAGTGGACC GCATCGACGT GGTGGTGGCC ACCACCCTTT CCATTACGGT GATCTCTGGG 
CAGTTCCTGT CAGAACGCAG CGTGCGCACC TATGTAGAAG TGGAGCTGTT TGGCCTTCCT 
GGGGACCCCA AGAGGCGCTA TCGAACTAAG CTGTCACCCA GTACTAACTC CATCAATCCT 
GTCTGGAAGG AGGAGCCCTT TGTCTTTGAG AAGATCTTGA TGCCTGAGCT GGCCTCCCTC 
AGAGTGGCTG TGATGGAGGA AGGCAACAAG TTTCTTGGAC ACCGCATCAT CCCCATCAAT 
GCCCTAAATT CTGGGTACCA CCACCTGTGC CTGCACAGTG AGAGCAACAT GCCCCTCACC 
ATGCCTGCGC TCTTCATCTT CCTGGAGATG AAGGACTACA TACCTGGTGC TTGGGCAGAT 
CTCACTGTGG CCCTCGCCAA CCCCATTAAG TTCTTCAGTG CCCATGACAC GAAGTCTGTG 
AAGCTCAAGG AGGCCATGGG AGGTCTGCCT GAGAAGCCCT TCCCACTGGC GAGTCCAGTT 
GCCAGCCAGG TCAATGGGGC GTTGGCCCCA ACGAGCAATG GGTCACCAGC AGCCAGGGCC 
GGGGCCAGGG AAGAGGCTAT GAAAGAAGCT GCGGAGCCGC GGACCGCCAG CCTGGAGGAG 
CTCCGGGAGC TAAAGGGCGT GGTGAAGCTG CAGCGGCGGC ACGAGAAGGA GCTGCGAGAG 
TTGGAGCGGC GCGGAGCGCG GCGCTGGGAG GAGCTGCTGC AGCGGGGCGC GGCGCAGCTG 
GCGGAGCTCG GGCCACCGGG CGTGGGGGGC GTCGGGGCCT GCAAGCTCGG TCCCGGCAAG 
GGCTCTCGCA AGAAGAGGAG CCTGCCCCGC GAGGAGAGCG CCGGAGCCGC GCCGGGCGAG 
GGCCCTGAGG GCGTGGACGG GCGCGTGCGG GAGCTGAAAG ACAGGCTGGA GCTGGAGCTG 
CTGCGGCAGG GCGAGGAGCA GTACGAGTGC GTTCTGAAGC GCAAGGAGCA GCACGTGGCC 
GAGCAAATCT CCAAAATGAT GGAGCTGGCC AGAGAGAAAC AGGCGGCAGA GCTGAAGGCC 
CTGAAGGAGA CGTCGGAGAA CGACACCAAA GAGATGAAGA AAAAGCTGGA GACAAAGAGA 
CTGGAGCGGA TCCAGGGCAT GACCAAAGTC ACCACAGACA AGATGGCCCA GGAGAGGTTG 
AAGAGAGAGA TTAACAACTC CCACATCCAG GAAGTAGTGC AGGTGATCAA GCAGATGACG 
GAGAACTTGG AGAGGCACCA GGAGAAGCTG GAGGAGAAGC AGGCGGCTTG CCTGGAACAG 
ATACGGGAGA TGGAAAAGCA GGGGTCCTTG CTGTCTCCAC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877805.1
CDS	277..3882
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022316.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGTGTGGG CTGGCGAGGA AGGGACTGAG 
CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT CAGGAAACCT GGATGAAGAA 
GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC TGGAAGTGAC GGCTTATGAG 
GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT TCGTCTCCTT TGAGTTCTCT 
GCCCCCCGGC TGTTTACCTG TATCTTCTCT GGACACAAGC ACAAAAAGAA CCGAAGTTAT 
GTCATCTCGT CCTTCACAGA GCTCAAGGCA TATGACCTGC TCTCCAAGGC CTCGGTGCAG 
TTTGTGGACT ACAACAAGCG CCAGATGAGC CGCATTTACC CCAAGGGAAC CCGCATGGAC 
TCCTCCAACT ACATGCCCCA GATGTTCTGG AATGCTGGAT GCCAGATGGT TGCCCTCAAC 
TTCCAGACGA TGGACTTGCC CATGCAGCAG AACATGGCAG TATTTGAGTT CAACGGGCAG 
AGCGGCTACC TCCTCAAGCA TGAGTTCATG CGCCGGCCGG ACAAGCAGTT CAACCCCTTC 
TCAGTGGACC GCATCGACGT GGTGGTGGCC ACCACCCTTT CCATTACGGT GATCTCTGGG 
CAGTTCCTGT CAGAACGCAG CGTGCGCACC TATGTAGAAG TGGAGCTGTT TGGCCTTCCT 
GGGGACCCCA AGAGGCGCTA TCGAACTAAG CTGTCACCCA GTACTAACTC CATCAATCCT 
GTCTGGAAGG AGGAGCCCTT TGTCTTTGAG AAGATCTTGA TGCCTGAGCT GGCCTCCCTC 
AGAGTGGCTG TGATGGAGGA AGGCAACAAG TTTCTTGGAC ACCGCATCAT CCCCATCAAT 
GCCCTAAATT CTGGGTACCA CCACCTGTGC CTGCACAGTG AGAGCAACAT GCCCCTCACC 
ATGCCTGCGC TCTTCATCTT CCTGGAGATG AAGGACTACA TACCTGGTGC TTGGGCAGAT 
CTCACTGTGG CCCTCGCCAA CCCCATTAAG TTCTTCAGTG CCCATGACAC GAAGTCTGTG 
AAGCTCAAGG AGGCCATGGG AGGTCTGCCT GAGAAGCCCT TCCCACTGGC GAGTCCAGTT 
GCCAGCCAGG TCAATGGGGC GTTGGCCCCA ACGAGCAATG GGTCACCAGC AGCCAGGGCC 
GGGGCCAGGG AAGAGGCTAT GAAAGAAGCT GCGGAGCCGC GGACCGCCAG CCTGGAGGAG 
CTCCGGGAGC TAAAGGGCGT GGTGAAGCTG CAGCGGCGGC ACGAGAAGGA GCTGCGAGAG 
TTGGAGCGGC GCGGAGCGCG GCGCTGGGAG GAGCTGCTGC AGCGGGGCGC GGCGCAGCTG 
GCGGAGCTCG GGCCACCGGG CGTGGGGGGC GTCGGGGCCT GCAAGCTCGG TCCCGGCAAG 
GGCTCTCGCA AGAAGAGGAG CCTGCCCCGC GAGGAGAGCG CCGGAGCCGC GCCGGGCGAG 
GGCCCTGAGG GCGTGGACGG GCGCGTGCGG GAGCTGAAAG ACAGGCTGGA GCTGGAGCTG 
CTGCGGCAGG GCGAGGAGCA GTACGAGTGC GTTCTGAAGC GCAAGGAGCA GCACGTGGCC 
GAGCAAATCT CCAAAATGAT GGAGCTGGCC AGAGAGAAAC AGGCGGCAGA GCTGAAGGCC 
CTGAAGGAGA CGTCGGAGAA CGACACCAAA GAGATGAAGA AAAAGCTGGA GACAAAGAGA 
CTGGAGCGGA TCCAGGGCAT GACCAAAGTC ACCACAGACA AGATGGCCCA GGAGAGGTTG 
AAGAGAGAGA TTAACAACTC CCACATCCAG GAAGTAGTGC AGGTGATCAA GCAGATGACG 
GAGAACTTGG AGAGGCACCA GGAGAAGCTG GAGGAGAAGC AGGCGGCTTG CCTGGAACAG 
ATACGGGAGA TGGAAAAGCA GGGGTCCTTG CTGTCTCCAC AGTTCCAGAA GGAGGCGCTG 
GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG TGAAGGAGTC GGTGAGGGCC 
TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC CTGAGAGGGC CTGCGAGTGC 
CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG CAGATGCCCA GGAGAGCCGC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877806.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu85497
Clone ID Related Accession (Same CDS sequence)	XM_017022317.1 , XM_017022317.2
Accession Version	XM_017022317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC 
GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG 
ACCTGCTTTC CCTCCGAGGC CAAGGACAAG CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG 
CTGTGTGAGC AGGACCCACT CATAGCAAAG GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877806.1
CDS	277..3873
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022317.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC 
GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG 
ACCTGCTTTC CCTCCGAGGC CAAGGACAAG CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG 
CTGTGTGAGC AGGACCCACT CATAGCAAAG GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022317.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877806.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.

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CloneID	OHu85497
Clone ID Related Accession (Same CDS sequence)	XM_017022317.1 , XM_017022317.2
Accession Version	XM_017022317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC 
GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG 
ACCTGCTTTC CCTCCGAGGC CAAGGACAAG CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG 
CTGTGTGAGC AGGACCCACT CATAGCAAAG GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877806.1
CDS	274..3870
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022317.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGTTCCAGA AGGAGGCGCT GGCAGAGTAC 
GAGGCCAGGA TGAAGGGTCT GGAGGCAGAG GTGAAGGAGT CGGTGAGGGC CTGCCTCAGG 
ACCTGCTTTC CCTCCGAGGC CAAGGACAAG CCTGAGAGGG CCTGCGAGTG CCCCCCAGAG 
CTGTGTGAGC AGGACCCACT CATAGCAAAG GCAGATGCCC AGGAGAGCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022318.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877807.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.

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CloneID	OHu85498
Clone ID Related Accession (Same CDS sequence)	XM_017022318.1
Accession Version	XM_017022318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GAGCCGCGGA CCGCCAGCCT GGAGGAGCTC CGGGAGCTAA AGGGCGTGGT GAAGCTGCAG 
CGGCGGCACG AGAAGGAGCT GCGAGAGTTG GAGCGGCGCG GAGCGCGGCG CTGGGAGGAG 
CTGCTGCAGC GGGGCGCGGC GCAGCTGGCG GAGCTCGGGC CACCGGGCGT GGGGGGCGTC 
GGGGCCTGCA AGCTCGGTCC CGGCAAGGGC TCTCGCAAGA AGAGGAGCCT GCCCCGCGAG 
GAGAGCGCCG GAGCCGCGCC GGGCGAGGGC CCTGAGGGCG TGGACGGGCG CGTGCGGGAG 
CTGAAAGACA GGCTGGAGCT GGAGCTGCTG CGGCAGGGCG AGGAGCAGTA CGAGTGCGTT 
CTGAAGCGCA AGGAGCAGCA CGTGGCCGAG CAAATCTCCA AAATGATGGA GCTGGCCAGA 
GAGAAACAGG CGGCAGAGCT GAAGGCCCTG AAGGAGACGT CGGAGAACGA CACCAAAGAG 
ATGAAGAAAA AGCTGGAGAC AAAGAGACTG GAGCGGATCC AGGGCATGAC CAAAGTCACC 
ACAGACAAGA TGGCCCAGGA GAGGTTGAAG AGAGAGATTA ACAACTCCCA CATCCAGGAA 
GTAGTGCAGG TGATCAAGCA GATGACGGAG AACTTGGAGA GGCACCAGGA GAAGCTGGAG 
GAGAAGCAGG CGGCTTGCCT GGAACAGATA CGGGAGATGG AAAAGCAGGG GTCCTTGCTG 
TCTCCACAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877807.1
CDS	277..3849
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQGSLLSPQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL

Target ORF information:

RefSeq Version	XM_017022318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022318.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GAGCCGCGGA CCGCCAGCCT GGAGGAGCTC CGGGAGCTAA AGGGCGTGGT GAAGCTGCAG 
CGGCGGCACG AGAAGGAGCT GCGAGAGTTG GAGCGGCGCG GAGCGCGGCG CTGGGAGGAG 
CTGCTGCAGC GGGGCGCGGC GCAGCTGGCG GAGCTCGGGC CACCGGGCGT GGGGGGCGTC 
GGGGCCTGCA AGCTCGGTCC CGGCAAGGGC TCTCGCAAGA AGAGGAGCCT GCCCCGCGAG 
GAGAGCGCCG GAGCCGCGCC GGGCGAGGGC CCTGAGGGCG TGGACGGGCG CGTGCGGGAG 
CTGAAAGACA GGCTGGAGCT GGAGCTGCTG CGGCAGGGCG AGGAGCAGTA CGAGTGCGTT 
CTGAAGCGCA AGGAGCAGCA CGTGGCCGAG CAAATCTCCA AAATGATGGA GCTGGCCAGA 
GAGAAACAGG CGGCAGAGCT GAAGGCCCTG AAGGAGACGT CGGAGAACGA CACCAAAGAG 
ATGAAGAAAA AGCTGGAGAC AAAGAGACTG GAGCGGATCC AGGGCATGAC CAAAGTCACC 
ACAGACAAGA TGGCCCAGGA GAGGTTGAAG AGAGAGATTA ACAACTCCCA CATCCAGGAA 
GTAGTGCAGG TGATCAAGCA GATGACGGAG AACTTGGAGA GGCACCAGGA GAAGCTGGAG 
GAGAAGCAGG CGGCTTGCCT GGAACAGATA CGGGAGATGG AAAAGCAGGG GTCCTTGCTG 
TCTCCACAGT TCCAGAAGGA GGCGCTGGCA GAGTACGAGG CCAGGATGAA GGGTCTGGAG 
GCAGAGGTGA AGGAGTCGGT GAGGGCCTGC CTCAGGACCT GCTTTCCCTC CGAGGCCAAG 
GACAAGCCTG AGAGGGCCTG CGAGTGCCCC CCAGAGCTGT GTGAGCAGGA CCCACTCATA 
GCAAAGGCAG ATGCCCAGGA GAGCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022319.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877808.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X8, mRNA.

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CloneID	OHu85499
Clone ID Related Accession (Same CDS sequence)	XM_017022319.2 , XM_017022319.1
Accession Version	XM_017022319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGAACATAC TGAGCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877808.1
CDS	274..3684
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQNILSP

Target ORF information:

RefSeq Version	XM_017022319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022319.2

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGAACATAC TGAGCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB2 ( XM_017022319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877808.1 Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.

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CloneID	OHu85499
Clone ID Related Accession (Same CDS sequence)	XM_017022319.2 , XM_017022319.1
Accession Version	XM_017022319.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGAACATAC TGAGCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877808.1
CDS	277..3687
Translation	MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAPRLFTCIFSGHKHKKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQNILSP

Target ORF information:

RefSeq Version	XM_017022319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C beta 2 (PLCB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022319.1

ORF Insert Sequence:

ATGTCTCTGC TCAACCCTGT CCTGCTGCCC CCCAAGGTGA AGGCCTATCT GAGCCAAGGG 
GAGCGCTTCA TCAAATGGGA TGATGAAACT ACAGTTGCCT CTCCAGTTAT CCTCCGTGTG 
GATCCTAAGG GCTACTACTT ATACTGGACG TATCAAAGTA AGGAGATGGA GTTTCTGGAT 
ATCACCAGCA TCCGGGATAC TCGCTTTGGG AAGTTTGCCA AGATGCCCAA GAGCCAGAAG 
CTCCGGGACG TCTTCAACAT GGACTTTCCT GATAACAGTT TCCTGCTGAA GACACTCACG 
GTGGTGTCCG GCCCGGACAT GGTGGACCTC ACCTTCCACA ACTTCGTCTC CTACAAGGAG 
AACGTGGGCA AGGCCTGGGC TGAGGACGTA CTGGCCCTAG TCAAACATCC GCTGACGGCC 
AACGCCTCCC GCAGCACCTT CCTGGACAAG ATCCTTGTGA AGCTCAAGAT GCAGCTCAAC 
TCTGAAGGGA AGATTCCGGT GAAGAACTTT TTCCAGATGT TTCCTGCTGA CCGCAAGCGG 
GTGGAAGCTG CTCTCAGTGC CTGCCACCTC CCCAAAGGCA AAAATGACGC CATCAATCCT 
GAGGACTTCC CAGAACCTGT CTACAAGAGT TTCCTCATGA GCCTCTGTCC TCGGCCAGAA 
ATAGATGAGA TCTTCACTTC TTACCATGCT AAGGCCAAAC CCTACATGAC GAAGGAGCAC 
CTGACCAAAT TCATCAACCA GAAACAGCGG GACTCCCGGC TTAACTCCCT GCTGTTCCCG 
CCAGCACGGC CTGACCAGGT GCAGGGCCTC ATCGACAAGT ATGAGCCCAG TGGCATCAAT 
GCACAGAGGG GCCAGCTGTC ACCTGAAGGC ATGGTCTGGT TTCTCTGTGG GCCAGAGAAC 
AGCGTGCTGG CCCAGGACAA GCTGCTGCTC CACCACGACA TGACGCAGCC ACTCAATCAT 
TACTTCATCA ACTCGTCCCA CAACACCTAC CTGACAGCCG GCCAGTTCTC AGGCCTCTCC 
TCGGCTGAGA TGTACCGCCA GGTGCTGCTC TCTGGCTGCC GTTGCGTGGA GCTAGACTGC 
TGGAAGGGGA AACCCCCTGA CGAGGAGCCC ATTATCACCC ATGGCTTCAC CATGACCACA 
GACATCTTCT TCAAAGAAGC AATTGAGGCT ATTGCAGAAA GCGCCTTTAA GACCTCCCCC 
TATCCCATCA TCCTGTCGTT TGAGAACCAT GTGGACTCAC CCCGCCAGCA GGCTAAGATG 
GCTGAGTATT GCCGGACGAT CTTTGGGGAT ATGCTGCTCA CAGAGCCCCT GGAAAAGTTC 
CCACTAAAAC CAGGTGTCCC CCTGCCCAGC CCTGAGGATC TCAGGGGCAA GATCCTCATC 
AAGAACAAGA AGAACCAGTT TTCTGGCCCC ACCTCCTCCA GTAAGGATAC TGGTGGGGAG 
GCTGAGGGCA GCAGCCCACC CAGTGCCCCT GCAGGTGAGG GCACAGTGTG GGCTGGCGAG 
GAAGGGACTG AGCTGGAGGA GGAGGAGGTG GAAGAGGAAG AGGAGGAGGA GTCAGGAAAC 
CTGGATGAAG AAGAGATTAA GAAGATGCAG TCGGATGAGG GCACAGCGGG CCTGGAAGTG 
ACGGCTTATG AGGAGATGTC CAGCCTAGTC AATTACATCC AGCCCACCAA GTTCGTCTCC 
TTTGAGTTCT CTGCCCCCCG GCTGTTTACC TGTATCTTCT CTGGACACAA GCACAAAAAG 
AACCGAAGTT ATGTCATCTC GTCCTTCACA GAGCTCAAGG CATATGACCT GCTCTCCAAG 
GCCTCGGTGC AGTTTGTGGA CTACAACAAG CGCCAGATGA GCCGCATTTA CCCCAAGGGA 
ACCCGCATGG ACTCCTCCAA CTACATGCCC CAGATGTTCT GGAATGCTGG ATGCCAGATG 
GTTGCCCTCA ACTTCCAGAC GATGGACTTG CCCATGCAGC AGAACATGGC AGTATTTGAG 
TTCAACGGGC AGAGCGGCTA CCTCCTCAAG CATGAGTTCA TGCGCCGGCC GGACAAGCAG 
TTCAACCCCT TCTCAGTGGA CCGCATCGAC GTGGTGGTGG CCACCACCCT TTCCATTACG 
GTGATCTCTG GGCAGTTCCT GTCAGAACGC AGCGTGCGCA CCTATGTAGA AGTGGAGCTG 
TTTGGCCTTC CTGGGGACCC CAAGAGGCGC TATCGAACTA AGCTGTCACC CAGTACTAAC 
TCCATCAATC CTGTCTGGAA GGAGGAGCCC TTTGTCTTTG AGAAGATCTT GATGCCTGAG 
CTGGCCTCCC TCAGAGTGGC TGTGATGGAG GAAGGCAACA AGTTTCTTGG ACACCGCATC 
ATCCCCATCA ATGCCCTAAA TTCTGGGTAC CACCACCTGT GCCTGCACAG TGAGAGCAAC 
ATGCCCCTCA CCATGCCTGC GCTCTTCATC TTCCTGGAGA TGAAGGACTA CATACCTGGT 
GCTTGGGCAG ATCTCACTGT GGCCCTCGCC AACCCCATTA AGTTCTTCAG TGCCCATGAC 
ACGAAGTCTG TGAAGCTCAA GGAGGCCATG GGAGGTCTGC CTGAGAAGCC CTTCCCACTG 
GCGAGTCCAG TTGCCAGCCA GGTCAATGGG GCGTTGGCCC CAACGAGCAA TGGGTCACCA 
GCAGCCAGGG CCGGGGCCAG GGAAGAGGCT ATGAAAGAAG CTGCGGAGCC GCGGACCGCC 
AGCCTGGAGG AGCTCCGGGA GCTAAAGGGC GTGGTGAAGC TGCAGCGGCG GCACGAGAAG 
GAGCTGCGAG AGTTGGAGCG GCGCGGAGCG CGGCGCTGGG AGGAGCTGCT GCAGCGGGGC 
GCGGCGCAGC TGGCGGAGCT CGGGCCACCG GGCGTGGGGG GCGTCGGGGC CTGCAAGCTC 
GGTCCCGGCA AGGGCTCTCG CAAGAAGAGG AGCCTGCCCC GCGAGGAGAG CGCCGGAGCC 
GCGCCGGGCG AGGGCCCTGA GGGCGTGGAC GGGCGCGTGC GGGAGCTGAA AGACAGGCTG 
GAGCTGGAGC TGCTGCGGCA GGGCGAGGAG CAGTACGAGT GCGTTCTGAA GCGCAAGGAG 
CAGCACGTGG CCGAGCAAAT CTCCAAAATG ATGGAGCTGG CCAGAGAGAA ACAGGCGGCA 
GAGCTGAAGG CCCTGAAGGA GACGTCGGAG AACGACACCA AAGAGATGAA GAAAAAGCTG 
GAGACAAAGA GACTGGAGCG GATCCAGGGC ATGACCAAAG TCACCACAGA CAAGATGGCC 
CAGGAGAGGT TGAAGAGAGA GATTAACAAC TCCCACATCC AGGAAGTAGT GCAGGTGATC 
AAGCAGATGA CGGAGAACTT GGAGAGGCAC CAGGAGAAGC TGGAGGAGAA GCAGGCGGCT 
TGCCTGGAAC AGATACGGGA GATGGAAAAG CAGAACATAC TGAGCCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Up-modulation of PLC-?2 reduces the number and malignancy of triple-negative breast tumor cells with a CD133
BMC cancer17(1)617(2017 Sep)
Brugnoli F,Grassilli S,Lanuti P,Marchisio M,Al-Qassab Y,Vezzali F,Capitani S,Bertagnolo V

PLC-?2 is modulated by low oxygen availability in breast tumor cells and plays a phenotype dependent role in their hypoxia-related malignant potential.
Molecular carcinogenesis55(12)2210-2221(2016 Dec)
Brugnoli F,Grassilli S,Al-Qassab Y,Capitani S,Bertagnolo V

Nuclear factor-?B regulates expression of platelet phospholipase C-?2 (PLCB2).
Thrombosis and haemostasis116(5)931-940(2016 Oct)
Mao G,Jin J,Kunapuli SP,Rao AK

Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.
Blood127(3)314-24(2016 Jan)
Block H,Stadtmann A,Riad D,Rossaint J,Sohlbach C,Germena G,Wu D,Simon SI,Ley K,Zarbock A

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PLCB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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