PLA2G4A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G4A
Entrez Gene ID	5321
Full Name	phospholipase A2 group IVA
Synonyms	PLA2G4,cPLA2,cPLA2-alpha
General protein information	Preferred Names phospholipase A2 group IVA Names cytosolic phospholipase A2 calcium-dependent phospholipid-binding protein lysophospholipase phosphatidylcholine 2-acylhydrolase phospholipase A2, group IVA (cytosolic, calcium-dependent)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q31.1
Summary	This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015].
Disorder MIM:	600522
Disorder Html:	Phospholipase A2, group IV A, deficiency of (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005245267.3	XP_005245324.2	cytosolic phospholipase A2 isoform X1
XM_005245267.4	XP_005245324.2	cytosolic phospholipase A2 isoform X1
NM_001311193.2	NP_001298122.2	cytosolic phospholipase A2 isoform 2
NM_001311193.1	NP_001298122.1	cytosolic phospholipase A2 isoform 2
NM_024420.2	NP_077734.1	cytosolic phospholipase A2 isoform 1
XM_011509642.2	XP_011507944.1	cytosolic phospholipase A2 isoform X2

Pathways from BioSystems

Homologies

	PLA2G4A	XP_001107479.2
Bos taurus (cattle)	PLA2G4A	NP_001069332.1
Gallus gallus (chicken)	PLA2G4A	NP_990754.1
Homo sapiens (human)	PLA2G4A	NP_077734.1
Xenopus tropicalis (tropical clawed frog)	pla2g4a	NP_001120577.1
Pan troglodytes (chimpanzee)	PLA2G4A	XP_001165694.1
Rattus norvegicus (Norway rat)	Pla2g4a	NP_598235.2
Danio rerio (zebrafish)	cpla2	NP_571370.1
Canis lupus familiaris (dog)	PLA2G4A	XP_005622510.1
Mus musculus (house mouse)	Pla2g4a	NP_032895.1
Danio rerio (zebrafish)	pla2g4a	XP_687485.3

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism.
Dessen A, Tang J, Schmidt H, Stahl M, Clark JD, Seehra J, Somers WS
Cell97(3)349-60(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these studies suggests that cPLA2alpha expressed by neutrophils, but not epithelial cells, plays a significant role in infection-induced neutrophil transepithelial migration by mediating LTB4 synthesis during migration, which serves to amplify the magnitude of neutrophil recruitment in response to epithelial infection
Title: Neutrophil-Derived Cytosolic PLA2? Contributes to Bacterial-Induced Neutrophil Transepithelial Migration.

cPLA2alpha activates PI3K/AKT and inhibits Smad2/3 during epithelial-mesenchymal transition of hepatocellular carcinoma cells.
Title: cPLA2? activates PI3K/AKT and inhibits Smad2/3 during epithelial-mesenchymal transition of hepatocellular carcinoma cells.

This study indicates the potential role of cPLA2alpha in breast cancer metastasis and indicates that this molecule is a promising therapeutic target for breast cancer.
Title: cPLA2? mediates TGF-?-induced epithelial-mesenchymal transition in breast cancer through PI3k/Akt signaling.

cPLA2alpha may play a key role in renal repair after injury through a PGE2-independent mechanism
Title: Cytosolic phospholipase A

present study indicates that among all cytosolic phospholipases A2, specific phospholipase A2 group IVA are the main enzymes involved in cigarette smoke induced anomalies in type I and type II lung epithelial cells
Title: Cytosolic phospholipase A2 (cPLA2) IVA as a potential signature molecule in cigarette smoke condensate induced pathologies in alveolar epithelial lineages.

The following PLA2G4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19957	NM_024420.2 Latest version!	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu59747	NM_001311193.1 Latest version!	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu59749	XM_011509642.2 Latest version!	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu108339	NM_001311193.2 Latest version!	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu90124	XM_005245267.4 Latest version!	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu90124	XM_005245267.3	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G4A ( NM_024420.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_077734.1 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19957
Clone ID Related Accession (Same CDS sequence)	NM_024420.2
Accession Version	NM_024420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022147.3, DA451491.1, BC114340.2, M72393.1 and BX118890.1. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2006 this sequence version replaced NM_024420.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M68874.1, SRR1660807.101293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT CACTGAAATG GTTCTAGAAA TGTCTCTTGA AGTTTGCTCA 
TGCCCAGACC TACGATTTAG TATGGCTCTG TGTGATCAGG AGAAGACTTT CAGACAACAG 
AGAAAAGAAC ACATAAGGGA GAGCATGAAG AAACTCTTGG GTCCAAAGAA TAGTGAAGGA 
TTGCATTCTG CACGTGATGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077734.1
CDS	206..2455
Translation	MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYKAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	NM_024420.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024420.2

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT CACTGAAATG GTTCTAGAAA TGTCTCTTGA AGTTTGCTCA 
TGCCCAGACC TACGATTTAG TATGGCTCTG TGTGATCAGG AGAAGACTTT CAGACAACAG 
AGAAAAGAAC ACATAAGGGA GAGCATGAAG AAACTCTTGG GTCCAAAGAA TAGTGAAGGA 
TTGCATTCTG CACGTGATGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( NM_001311193.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001298122.1 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu59747
Clone ID Related Accession (Same CDS sequence)	NM_001311193.1
Accession Version	NM_001311193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022147.3, AK302938.1, BC114340.2 and BX118890.1. Transcript Variant: This variant (2) lacks two in-frame exons in the central coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.5588.1, AK302938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298122.1
CDS	206..2275
Translation	MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYKAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	NM_001311193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311193.1

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( XM_011509642.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507944.1 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X2, mRNA.

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CloneID	OHu59749
Clone ID Related Accession (Same CDS sequence)	XM_011509642.2
Accession Version	XM_011509642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT CACTGAAATG GTTCTAGAAA TGTCTCTTGA AGTTTGCTCA 
TGCCCAGACC TACGATTTAG TATGGCTCTG TGTGATCAGG AGAAGACTTT CAGACAACAG 
AGAAAAGAAC ACATAAGGGA GAGCATGAAG AAACTCTTGG GTCCAAAGAA TAGTGAAGGA 
TTGCATTCTG CACGTGATGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AGGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507944.1
CDS	157..2406
Translation	MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	XM_011509642.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509642.2

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT CACTGAAATG GTTCTAGAAA TGTCTCTTGA AGTTTGCTCA 
TGCCCAGACC TACGATTTAG TATGGCTCTG TGTGATCAGG AGAAGACTTT CAGACAACAG 
AGAAAAGAAC ACATAAGGGA GAGCATGAAG AAACTCTTGG GTCCAAAGAA TAGTGAAGGA 
TTGCATTCTG CACGTGATGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AGGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( NM_001311193.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001298122.2 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.

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CloneID	OHu108339
Clone ID Related Accession (Same CDS sequence)	NM_001311193.2
Accession Version	NM_001311193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022147.3 and AL049797.8. On Jun 2, 2019 this sequence version replaced NM_001311193.1. Transcript Variant: This variant (2) lacks two in-frame exons in the central coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.5588.1, AK302938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AGGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298122.2
CDS	157..2226
Translation	MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	NM_001311193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311193.2

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT 
ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT 
ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA 
AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA 
ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT 
CCTTTTATTT TCAACCAAGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA 
GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT 
GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC 
CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG 
AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA 
GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT 
ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG 
TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG 
TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA 
TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA 
TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT 
CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT 
ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA 
CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC 
AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG 
ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA 
AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC 
TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AGGGCTCCAG GTGTTCCAAG GGAAACTGAG 
GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC 
TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT 
ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA 
CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC 
AAGGAGTTTC TAAGTAAACC CAAAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( XM_005245267.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245324.2 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

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CloneID	OHu90124
Clone ID Related Accession (Same CDS sequence)	XM_005245267.3 , XM_005245267.4
Accession Version	XM_005245267.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245267.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTAT GTTTGTCTAG ATCTTTGAAT GATAACATTT CAAACAGTTC TCTGATGCTA 
TCTGTCAGAT TAGCATTTTA CCTGGGCCTT AAATTAGCCA AAGTGACAAA GGGGGCCTTT 
GGTGACATGC TTGATACTCC AGATCCCTAT GTGGAACTTT TTATCTCTAC AACCCCTGAC 
AGCAGGAAGA GAACAAGACA TTTCAATAAT GACATAAACC CTGTGTGGAA TGAGACCTTT 
GAATTTATTT TGGATCCTAA TCAGGAAAAT GTTTTGGAGA TTACGTTAAT GGATGCCAAT 
TATGTCATGG ATGAAACTCT AGGGACAGCA ACATTTACTG TATCTTCTAT GAAGGTGGGA 
GAAAAGAAAG AAGTTCCTTT TATTTTCAAC CAAGTCACTG AAATGGTTCT AGAAATGTCT 
CTTGAAGTTT GCTCATGCCC AGACCTACGA TTTAGTATGG CTCTGTGTGA TCAGGAGAAG 
ACTTTCAGAC AACAGAGAAA AGAACACATA AGGGAGAGCA TGAAGAAACT CTTGGGTCCA 
AAGAATAGTG AAGGATTGCA TTCTGCACGT GATGTGCCTG TGGTAGCCAT ATTGGGTTCA 
GGTGGGGGTT TCCGAGCCAT GGTGGGATTC TCTGGTGTGA TGAAGGCATT ATACGAATCA 
GGAATTCTGG ATTGTGCTAC CTACGTTGCT GGTCTTTCTG GCTCCACCTG GTATATGTCA 
ACCTTGTATT CTCACCCTGA TTTTCCAGAG AAAGGGCCAG AGGAGATTAA TGAAGAACTA 
ATGAAAAATG TTAGCCACAA TCCCCTTTTA CTTCTCACAC CACAGAAAGT TAAAAGATAT 
GTTGAGTCTT TATGGAAGAA GAAAAGCTCT GGACAACCTG TCACCTTTAC TGATATCTTT 
GGGATGTTAA TAGGAGAAAC ACTAATTCAT AATAGAATGA ATACTACTCT GAGCAGTTTG 
AAGGAAAAAG TTAATACTGC ACAATGCCCT TTACCTCTTT TCACCTGTCT TCATGTCAAA 
CCTGACGTTT CAGAGCTGAT GTTTGCAGAT TGGGTTGAAT TTAGTCCATA CGAAATTGGC 
ATGGCTAAAT ATGGTACTTT TATGGCTCCC GACTTATTTG GAAGCAAATT TTTTATGGGA 
ACAGTCGTTA AGAAGTATGA AGAAAACCCC TTGCATTTCT TAATGGGTGT CTGGGGCAGT 
GCCTTTTCCA TATTGTTCAA CAGAGTTTTG GGCGTTTCTG GTTCACAAAG CAGAGGCTCC 
ACAATGGAGG AAGAATTAGA AAATATTACC ACAAAGCATA TTGTGAGTAA TGATAGCTCG 
GACAGTGATG ATGAATCACA CGAACCCAAA GGCACTGAAA ATGAAGATGC TGGAAGTGAC 
TATCAAAGTG ATAATCAAGC AAGTTGGATT CATCGTATGA TAATGGCCTT GGTGAGTGAT 
TCAGCTTTAT TCAATACCAG AGAAGGACGT GCTGGGAAGG TACACAACTT CATGCTGGGC 
TTGAATCTCA ATACATCTTA TCCACTGTCT CCTTTGAGTG ACTTTGCCAC ACAGGACTCC 
TTTGATGATG ATGAACTGGA TGCAGCTGTA GCAGATCCTG ATGAATTTGA GCGAATATAT 
GAGCCTCTGG ATGTCAAAAG TAAAAAGATT CATGTAGTGG ACAGTGGGCT CACATTTAAC 
CTGCCGTATC CCTTGATACT GAGACCTCAG AGAGGGGTTG ATCTCATAAT CTCCTTTGAC 
TTTTCTGCAA GGCCAAGTGA CTCTAGTCCT CCGTTCAAGG AACTTCTACT TGCAGAAAAG 
TGGGCTAAAA TGAACAAGCT CCCCTTTCCA AAGATTGATC CTTATGTGTT TGATCGGGAA 
GGGCTGAAGG AGTGCTATGT CTTTAAACCC AAGAATCCTG ATATGGAGAA AGATTGCCCA 
ACCATCATCC ACTTTGTTCT GGCCAACATC AACTTCAGAA AGTACAGGGC TCCAGGTGTT 
CCAAGGGAAA CTGAGGAAGA GAAAGAAATC GCTGACTTTG ATATTTTTGA TGACCCAGAA 
TCACCATTTT CAACCTTCAA TTTTCAATAT CCAAATCAAG CATTCAAAAG ACTACATGAT 
CTTATGCACT TCAATACTCT GAACAACATT GATGTGATAA AAGAAGCCAT GGTTGAAAGC 
ATTGAATATA GAAGACAGAA TCCATCTCGT TGCTCTGTTT CCCTTAGTAA TGTTGAGGCA 
AGAAGATTTT TCAACAAGGA GTTTCTAAGT AAACCCAAAG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245324.2
CDS	12..2276
Translation	MWLCLSRSLNDNISNSSLMLSVRLAFYLGLKLAKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	XM_005245267.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245267.3

ORF Insert Sequence:

ATGTGGTTAT GTTTGTCTAG ATCTTTGAAT GATAACATTT CAAACAGTTC TCTGATGCTA 
TCTGTCAGAT TAGCATTTTA CCTGGGCCTT AAATTAGCCA AAGTGACAAA GGGGGCCTTT 
GGTGACATGC TTGATACTCC AGATCCCTAT GTGGAACTTT TTATCTCTAC AACCCCTGAC 
AGCAGGAAGA GAACAAGACA TTTCAATAAT GACATAAACC CTGTGTGGAA TGAGACCTTT 
GAATTTATTT TGGATCCTAA TCAGGAAAAT GTTTTGGAGA TTACGTTAAT GGATGCCAAT 
TATGTCATGG ATGAAACTCT AGGGACAGCA ACATTTACTG TATCTTCTAT GAAGGTGGGA 
GAAAAGAAAG AAGTTCCTTT TATTTTCAAC CAAGTCACTG AAATGGTTCT AGAAATGTCT 
CTTGAAGTTT GCTCATGCCC AGACCTACGA TTTAGTATGG CTCTGTGTGA TCAGGAGAAG 
ACTTTCAGAC AACAGAGAAA AGAACACATA AGGGAGAGCA TGAAGAAACT CTTGGGTCCA 
AAGAATAGTG AAGGATTGCA TTCTGCACGT GATGTGCCTG TGGTAGCCAT ATTGGGTTCA 
GGTGGGGGTT TCCGAGCCAT GGTGGGATTC TCTGGTGTGA TGAAGGCATT ATACGAATCA 
GGAATTCTGG ATTGTGCTAC CTACGTTGCT GGTCTTTCTG GCTCCACCTG GTATATGTCA 
ACCTTGTATT CTCACCCTGA TTTTCCAGAG AAAGGGCCAG AGGAGATTAA TGAAGAACTA 
ATGAAAAATG TTAGCCACAA TCCCCTTTTA CTTCTCACAC CACAGAAAGT TAAAAGATAT 
GTTGAGTCTT TATGGAAGAA GAAAAGCTCT GGACAACCTG TCACCTTTAC TGATATCTTT 
GGGATGTTAA TAGGAGAAAC ACTAATTCAT AATAGAATGA ATACTACTCT GAGCAGTTTG 
AAGGAAAAAG TTAATACTGC ACAATGCCCT TTACCTCTTT TCACCTGTCT TCATGTCAAA 
CCTGACGTTT CAGAGCTGAT GTTTGCAGAT TGGGTTGAAT TTAGTCCATA CGAAATTGGC 
ATGGCTAAAT ATGGTACTTT TATGGCTCCC GACTTATTTG GAAGCAAATT TTTTATGGGA 
ACAGTCGTTA AGAAGTATGA AGAAAACCCC TTGCATTTCT TAATGGGTGT CTGGGGCAGT 
GCCTTTTCCA TATTGTTCAA CAGAGTTTTG GGCGTTTCTG GTTCACAAAG CAGAGGCTCC 
ACAATGGAGG AAGAATTAGA AAATATTACC ACAAAGCATA TTGTGAGTAA TGATAGCTCG 
GACAGTGATG ATGAATCACA CGAACCCAAA GGCACTGAAA ATGAAGATGC TGGAAGTGAC 
TATCAAAGTG ATAATCAAGC AAGTTGGATT CATCGTATGA TAATGGCCTT GGTGAGTGAT 
TCAGCTTTAT TCAATACCAG AGAAGGACGT GCTGGGAAGG TACACAACTT CATGCTGGGC 
TTGAATCTCA ATACATCTTA TCCACTGTCT CCTTTGAGTG ACTTTGCCAC ACAGGACTCC 
TTTGATGATG ATGAACTGGA TGCAGCTGTA GCAGATCCTG ATGAATTTGA GCGAATATAT 
GAGCCTCTGG ATGTCAAAAG TAAAAAGATT CATGTAGTGG ACAGTGGGCT CACATTTAAC 
CTGCCGTATC CCTTGATACT GAGACCTCAG AGAGGGGTTG ATCTCATAAT CTCCTTTGAC 
TTTTCTGCAA GGCCAAGTGA CTCTAGTCCT CCGTTCAAGG AACTTCTACT TGCAGAAAAG 
TGGGCTAAAA TGAACAAGCT CCCCTTTCCA AAGATTGATC CTTATGTGTT TGATCGGGAA 
GGGCTGAAGG AGTGCTATGT CTTTAAACCC AAGAATCCTG ATATGGAGAA AGATTGCCCA 
ACCATCATCC ACTTTGTTCT GGCCAACATC AACTTCAGAA AGTACAGGGC TCCAGGTGTT 
CCAAGGGAAA CTGAGGAAGA GAAAGAAATC GCTGACTTTG ATATTTTTGA TGACCCAGAA 
TCACCATTTT CAACCTTCAA TTTTCAATAT CCAAATCAAG CATTCAAAAG ACTACATGAT 
CTTATGCACT TCAATACTCT GAACAACATT GATGTGATAA AAGAAGCCAT GGTTGAAAGC 
ATTGAATATA GAAGACAGAA TCCATCTCGT TGCTCTGTTT CCCTTAGTAA TGTTGAGGCA 
AGAAGATTTT TCAACAAGGA GTTTCTAAGT AAACCCAAAG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( XM_005245267.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245324.2 Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

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CloneID	OHu90124
Clone ID Related Accession (Same CDS sequence)	XM_005245267.3 , XM_005245267.4
Accession Version	XM_005245267.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic phospholipase A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245267.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTAT GTTTGTCTAG ATCTTTGAAT GATAACATTT CAAACAGTTC TCTGATGCTA 
TCTGTCAGAT TAGCATTTTA CCTGGGCCTT AAATTAGCCA AAGTGACAAA GGGGGCCTTT 
GGTGACATGC TTGATACTCC AGATCCCTAT GTGGAACTTT TTATCTCTAC AACCCCTGAC 
AGCAGGAAGA GAACAAGACA TTTCAATAAT GACATAAACC CTGTGTGGAA TGAGACCTTT 
GAATTTATTT TGGATCCTAA TCAGGAAAAT GTTTTGGAGA TTACGTTAAT GGATGCCAAT 
TATGTCATGG ATGAAACTCT AGGGACAGCA ACATTTACTG TATCTTCTAT GAAGGTGGGA 
GAAAAGAAAG AAGTTCCTTT TATTTTCAAC CAAGTCACTG AAATGGTTCT AGAAATGTCT 
CTTGAAGTTT GCTCATGCCC AGACCTACGA TTTAGTATGG CTCTGTGTGA TCAGGAGAAG 
ACTTTCAGAC AACAGAGAAA AGAACACATA AGGGAGAGCA TGAAGAAACT CTTGGGTCCA 
AAGAATAGTG AAGGATTGCA TTCTGCACGT GATGTGCCTG TGGTAGCCAT ATTGGGTTCA 
GGTGGGGGTT TCCGAGCCAT GGTGGGATTC TCTGGTGTGA TGAAGGCATT ATACGAATCA 
GGAATTCTGG ATTGTGCTAC CTACGTTGCT GGTCTTTCTG GCTCCACCTG GTATATGTCA 
ACCTTGTATT CTCACCCTGA TTTTCCAGAG AAAGGGCCAG AGGAGATTAA TGAAGAACTA 
ATGAAAAATG TTAGCCACAA TCCCCTTTTA CTTCTCACAC CACAGAAAGT TAAAAGATAT 
GTTGAGTCTT TATGGAAGAA GAAAAGCTCT GGACAACCTG TCACCTTTAC TGATATCTTT 
GGGATGTTAA TAGGAGAAAC ACTAATTCAT AATAGAATGA ATACTACTCT GAGCAGTTTG 
AAGGAAAAAG TTAATACTGC ACAATGCCCT TTACCTCTTT TCACCTGTCT TCATGTCAAA 
CCTGACGTTT CAGAGCTGAT GTTTGCAGAT TGGGTTGAAT TTAGTCCATA CGAAATTGGC 
ATGGCTAAAT ATGGTACTTT TATGGCTCCC GACTTATTTG GAAGCAAATT TTTTATGGGA 
ACAGTCGTTA AGAAGTATGA AGAAAACCCC TTGCATTTCT TAATGGGTGT CTGGGGCAGT 
GCCTTTTCCA TATTGTTCAA CAGAGTTTTG GGCGTTTCTG GTTCACAAAG CAGAGGCTCC 
ACAATGGAGG AAGAATTAGA AAATATTACC ACAAAGCATA TTGTGAGTAA TGATAGCTCG 
GACAGTGATG ATGAATCACA CGAACCCAAA GGCACTGAAA ATGAAGATGC TGGAAGTGAC 
TATCAAAGTG ATAATCAAGC AAGTTGGATT CATCGTATGA TAATGGCCTT GGTGAGTGAT 
TCAGCTTTAT TCAATACCAG AGAAGGACGT GCTGGGAAGG TACACAACTT CATGCTGGGC 
TTGAATCTCA ATACATCTTA TCCACTGTCT CCTTTGAGTG ACTTTGCCAC ACAGGACTCC 
TTTGATGATG ATGAACTGGA TGCAGCTGTA GCAGATCCTG ATGAATTTGA GCGAATATAT 
GAGCCTCTGG ATGTCAAAAG TAAAAAGATT CATGTAGTGG ACAGTGGGCT CACATTTAAC 
CTGCCGTATC CCTTGATACT GAGACCTCAG AGAGGGGTTG ATCTCATAAT CTCCTTTGAC 
TTTTCTGCAA GGCCAAGTGA CTCTAGTCCT CCGTTCAAGG AACTTCTACT TGCAGAAAAG 
TGGGCTAAAA TGAACAAGCT CCCCTTTCCA AAGATTGATC CTTATGTGTT TGATCGGGAA 
GGGCTGAAGG AGTGCTATGT CTTTAAACCC AAGAATCCTG ATATGGAGAA AGATTGCCCA 
ACCATCATCC ACTTTGTTCT GGCCAACATC AACTTCAGAA AGTACAGGGC TCCAGGTGTT 
CCAAGGGAAA CTGAGGAAGA GAAAGAAATC GCTGACTTTG ATATTTTTGA TGACCCAGAA 
TCACCATTTT CAACCTTCAA TTTTCAATAT CCAAATCAAG CATTCAAAAG ACTACATGAT 
CTTATGCACT TCAATACTCT GAACAACATT GATGTGATAA AAGAAGCCAT GGTTGAAAGC 
ATTGAATATA GAAGACAGAA TCCATCTCGT TGCTCTGTTT CCCTTAGTAA TGTTGAGGCA 
AGAAGATTTT TCAACAAGGA GTTTCTAAGT AAACCCAAAG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245324.2
CDS	12..2276
Translation	MWLCLSRSLNDNISNSSLMLSVRLAFYLGLKLAKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA

Target ORF information:

RefSeq Version	XM_005245267.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245267.4

ORF Insert Sequence:

ATGTGGTTAT GTTTGTCTAG ATCTTTGAAT GATAACATTT CAAACAGTTC TCTGATGCTA 
TCTGTCAGAT TAGCATTTTA CCTGGGCCTT AAATTAGCCA AAGTGACAAA GGGGGCCTTT 
GGTGACATGC TTGATACTCC AGATCCCTAT GTGGAACTTT TTATCTCTAC AACCCCTGAC 
AGCAGGAAGA GAACAAGACA TTTCAATAAT GACATAAACC CTGTGTGGAA TGAGACCTTT 
GAATTTATTT TGGATCCTAA TCAGGAAAAT GTTTTGGAGA TTACGTTAAT GGATGCCAAT 
TATGTCATGG ATGAAACTCT AGGGACAGCA ACATTTACTG TATCTTCTAT GAAGGTGGGA 
GAAAAGAAAG AAGTTCCTTT TATTTTCAAC CAAGTCACTG AAATGGTTCT AGAAATGTCT 
CTTGAAGTTT GCTCATGCCC AGACCTACGA TTTAGTATGG CTCTGTGTGA TCAGGAGAAG 
ACTTTCAGAC AACAGAGAAA AGAACACATA AGGGAGAGCA TGAAGAAACT CTTGGGTCCA 
AAGAATAGTG AAGGATTGCA TTCTGCACGT GATGTGCCTG TGGTAGCCAT ATTGGGTTCA 
GGTGGGGGTT TCCGAGCCAT GGTGGGATTC TCTGGTGTGA TGAAGGCATT ATACGAATCA 
GGAATTCTGG ATTGTGCTAC CTACGTTGCT GGTCTTTCTG GCTCCACCTG GTATATGTCA 
ACCTTGTATT CTCACCCTGA TTTTCCAGAG AAAGGGCCAG AGGAGATTAA TGAAGAACTA 
ATGAAAAATG TTAGCCACAA TCCCCTTTTA CTTCTCACAC CACAGAAAGT TAAAAGATAT 
GTTGAGTCTT TATGGAAGAA GAAAAGCTCT GGACAACCTG TCACCTTTAC TGATATCTTT 
GGGATGTTAA TAGGAGAAAC ACTAATTCAT AATAGAATGA ATACTACTCT GAGCAGTTTG 
AAGGAAAAAG TTAATACTGC ACAATGCCCT TTACCTCTTT TCACCTGTCT TCATGTCAAA 
CCTGACGTTT CAGAGCTGAT GTTTGCAGAT TGGGTTGAAT TTAGTCCATA CGAAATTGGC 
ATGGCTAAAT ATGGTACTTT TATGGCTCCC GACTTATTTG GAAGCAAATT TTTTATGGGA 
ACAGTCGTTA AGAAGTATGA AGAAAACCCC TTGCATTTCT TAATGGGTGT CTGGGGCAGT 
GCCTTTTCCA TATTGTTCAA CAGAGTTTTG GGCGTTTCTG GTTCACAAAG CAGAGGCTCC 
ACAATGGAGG AAGAATTAGA AAATATTACC ACAAAGCATA TTGTGAGTAA TGATAGCTCG 
GACAGTGATG ATGAATCACA CGAACCCAAA GGCACTGAAA ATGAAGATGC TGGAAGTGAC 
TATCAAAGTG ATAATCAAGC AAGTTGGATT CATCGTATGA TAATGGCCTT GGTGAGTGAT 
TCAGCTTTAT TCAATACCAG AGAAGGACGT GCTGGGAAGG TACACAACTT CATGCTGGGC 
TTGAATCTCA ATACATCTTA TCCACTGTCT CCTTTGAGTG ACTTTGCCAC ACAGGACTCC 
TTTGATGATG ATGAACTGGA TGCAGCTGTA GCAGATCCTG ATGAATTTGA GCGAATATAT 
GAGCCTCTGG ATGTCAAAAG TAAAAAGATT CATGTAGTGG ACAGTGGGCT CACATTTAAC 
CTGCCGTATC CCTTGATACT GAGACCTCAG AGAGGGGTTG ATCTCATAAT CTCCTTTGAC 
TTTTCTGCAA GGCCAAGTGA CTCTAGTCCT CCGTTCAAGG AACTTCTACT TGCAGAAAAG 
TGGGCTAAAA TGAACAAGCT CCCCTTTCCA AAGATTGATC CTTATGTGTT TGATCGGGAA 
GGGCTGAAGG AGTGCTATGT CTTTAAACCC AAGAATCCTG ATATGGAGAA AGATTGCCCA 
ACCATCATCC ACTTTGTTCT GGCCAACATC AACTTCAGAA AGTACAGGGC TCCAGGTGTT 
CCAAGGGAAA CTGAGGAAGA GAAAGAAATC GCTGACTTTG ATATTTTTGA TGACCCAGAA 
TCACCATTTT CAACCTTCAA TTTTCAATAT CCAAATCAAG CATTCAAAAG ACTACATGAT 
CTTATGCACT TCAATACTCT GAACAACATT GATGTGATAA AAGAAGCCAT GGTTGAAAGC 
ATTGAATATA GAAGACAGAA TCCATCTCGT TGCTCTGTTT CCCTTAGTAA TGTTGAGGCA 
AGAAGATTTT TCAACAAGGA GTTTCTAAGT AAACCCAAAG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism.
Cell97(3)349-60(1999 Apr)
Dessen A,Tang J,Schmidt H,Stahl M,Clark JD,Seehra J,Somers WS

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PLA2G4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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