PIGF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIGF
Entrez Gene ID	5281
Full Name	phosphatidylinositol glycan anchor biosynthesis class F
General protein information	Preferred Names phosphatidylinositol glycan anchor biosynthesis class F Names phosphatidylinositol-glycan biosynthesis class F protein GPI11 homolog PIG-F
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p21
Summary	This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	600153

mRNA and Protein(s)

mRNA	Protein	Name
XM_011532908.2	XP_011531210.1	phosphatidylinositol-glycan biosynthesis class F protein isoform X1
NM_173074.2	NP_775097.1	phosphatidylinositol-glycan biosynthesis class F protein isoform 2
XM_011532908.3	XP_011531210.1	phosphatidylinositol-glycan biosynthesis class F protein isoform X1
XM_005264369.3	XP_005264426.1	phosphatidylinositol-glycan biosynthesis class F protein isoform X2
XM_005264369.2	XP_005264426.1	phosphatidylinositol-glycan biosynthesis class F protein isoform X2
NM_002643.3	NP_002634.1	phosphatidylinositol-glycan biosynthesis class F protein isoform 1
NM_002643.4	NP_002634.1	phosphatidylinositol-glycan biosynthesis class F protein isoform 1
NM_173074.3	NP_775097.1	phosphatidylinositol-glycan biosynthesis class F protein isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIGF	NP_002634.1
Canis lupus familiaris (dog)	PIGF	XP_531809.2
Xenopus tropicalis (tropical clawed frog)	pigf	NP_001016071.1
Bos taurus (cattle)	PIGF	NP_001070542.1
Mus musculus (house mouse)	Pigf	NP_032864.1
Rattus norvegicus (Norway rat)	Pigf	XP_006239787.1
Danio rerio (zebrafish)	pigf	NP_991208.1
	LOC714844	XP_001113130.2
Gallus gallus (chicken)	PIGF	XP_003641023.1
Pan troglodytes (chimpanzee)	PIGF	XP_001148023.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhanced HIF2? expression during human trophoblast differentiation into syncytiotrophoblast suppresses transcription of placental growth factor.
Fujii T, Nagamatsu T, Morita K, Schust DJ, Iriyama T, Komatsu A, Osuga Y, Fujii T
Scientific reports7(1)12455(2017 Sep)

New insights into the functions of PIGF, a protein involved in the ethanolamine phosphate transfer steps of glycosylphosphatidylinositol biosynthesis.
Stokes MJ, Murakami Y, Maeda Y, Kinoshita T, Morita YS
The Biochemical journal463(2)249-56(2014 Oct)

Retinal reactive astrocytic tumor: gene expression profiling.
Shehri M, Bouhenni R, Ghazi NG, Abu Safieh L, Edward DP
JAMA ophthalmology132(6)773-5(2014 Jun)

Identification of type 2 diabetes-associated combination of SNPs using support vector machine.
Ban HJ, Heo JY, Oh KS, Park KJ
BMC genetics1126(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study investigated the interplay of VEGF-A165a isoform, the anti-angiogenic VEGF-A165b, placental growth factor (PIGF) and their receptors, VEGFR1 and VEGFR2.on junctional occupancy of VE-cadherin and macromolecular leakage in human endothelial monolayers and the perfused placental microvascular bed.
Title: Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A

The early variations of PIGF and soluble fms-like tyrosine kinase-1 concentrations in newly pregnant obstetric antiphospholipid syndrome (oAPS) may help to detect patients at low risk of placenta-mediated complications (PMC).
Title: Obstetric antiphospholipid syndrome: early variations of angiogenic factors are associated with adverse outcomes.

knockdown of PlGF in spheroid body cells derived from two gastric cancer cell lines reduced in vitro tumorigenicity and stemness properties of spheroid body cells such as self-renewal ability, colony forming, migratory, and MMPs activities and decreased ability to differentiation and angiogenesis
Title: PlGF Knockdown Decreases Tumorigenicity and Stemness Properties of Spheroid Body Cells Derived from Gastric Cancer Cells.

Lower PlGF and higher PAPP-A and free beta-hCG levels were found in the fetal circulation of near-term severe preeclamptic pregnancies
Title: Lower placental growth factor and higher free ?-hCG and PAPP-A levels in the fetal circulation of near-term pregnancies complicated with severe preeclampsia.

There is a significant negative correlation between the concentration of sFLt-1 and PlGF in normal pregnancy.
Title: Difference of concentration of placental soluble fms-like tyrosine kinase-1(sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in severe preeclampsia and normal pregnancy.

The following PIGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13287	NM_173074.3 Latest version!	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu13366	NM_002643.4 Latest version!	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu35437	XM_005264369.3 Latest version!	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu59682	XM_011532908.3 Latest version!	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu59682	XM_011532908.2	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu35437	XM_005264369.2	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu13366	NM_002643.3	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu13287	NM_173074.2	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIGF ( NM_173074.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_775097.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13287
Clone ID Related Accession (Same CDS sequence)	NM_173074.2 , NM_173074.3
Accession Version	NM_173074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-06
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018682.4 and BC029408.1. On Jul 6, 2006 this sequence version replaced NM_173074.1. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC021725.1, SRR1163658.382772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGATGA CAGTAGAAAG AAAACGTAGC ACTTATAGAT CTCTCCATGT ACCCTGCAGG 
GGGCTTGGTA CTGTGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775097.1
CDS	165..785
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQMTVERKRSTYRSLHVPCRGLGTVK

Target ORF information:

RefSeq Version	NM_173074.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173074.2

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGATGA CAGTAGAAAG AAAACGTAGC ACTTATAGAT CTCTCCATGT ACCCTGCAGG 
GGGCTTGGTA CTGTGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( NM_173074.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775097.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13287
Clone ID Related Accession (Same CDS sequence)	NM_173074.2 , NM_173074.3
Accession Version	NM_173074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018682.4 and BC029408.1. On May 31, 2019 this sequence version replaced NM_173074.2. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021725.1, SRR1163658.382772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGATGA CAGTAGAAAG AAAACGTAGC ACTTATAGAT CTCTCCATGT ACCCTGCAGG 
GGGCTTGGTA CTGTGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775097.1
CDS	94..714
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQMTVERKRSTYRSLHVPCRGLGTVK

Target ORF information:

RefSeq Version	NM_173074.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173074.3

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGATGA CAGTAGAAAG AAAACGTAGC ACTTATAGAT CTCTCCATGT ACCCTGCAGG 
GGGCTTGGTA CTGTGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( NM_002643.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002634.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13366
Clone ID Related Accession (Same CDS sequence)	NM_002643.3 , NM_002643.4
Accession Version	NM_002643.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-06
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018682.4 and BC029408.1. On Jul 6, 2006 this sequence version replaced NM_002643.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC029408.1, SRR1163658.226583.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGTAT GGCCCATCTC CTGTACGCTT GGAGCGACCT TTGGCTACGT GGCTGGCCTT 
GTTATTTCAC CACTCTGGAT ATACTGGAAT AGAAAGCAAC TTACATACAA GAACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002634.1
CDS	165..824
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN

Target ORF information:

RefSeq Version	NM_002643.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002643.3

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGTAT GGCCCATCTC CTGTACGCTT GGAGCGACCT TTGGCTACGT GGCTGGCCTT 
GTTATTTCAC CACTCTGGAT ATACTGGAAT AGAAAGCAAC TTACATACAA GAACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( NM_002643.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002634.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13366
Clone ID Related Accession (Same CDS sequence)	NM_002643.3 , NM_002643.4
Accession Version	NM_002643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018682.4 and BC029408.1. On May 17, 2019 this sequence version replaced NM_002643.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.439290.1, SRR1163657.60128.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000281382.11/ ENSP00000281382.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGTAT GGCCCATCTC CTGTACGCTT GGAGCGACCT TTGGCTACGT GGCTGGCCTT 
GTTATTTCAC CACTCTGGAT ATACTGGAAT AGAAAGCAAC TTACATACAA GAACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002634.1
CDS	94..753
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN

Target ORF information:

RefSeq Version	NM_002643.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002643.4

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGTAT GGCCCATCTC CTGTACGCTT GGAGCGACCT TTGGCTACGT GGCTGGCCTT 
GTTATTTCAC CACTCTGGAT ATACTGGAAT AGAAAGCAAC TTACATACAA GAACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( XM_005264369.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264426.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35437
Clone ID Related Accession (Same CDS sequence)	XM_005264369.3 , XM_005264369.2
Accession Version	XM_005264369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGAAGC AGATAGCAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264426.1
CDS	110..673
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQKQIAR

Target ORF information:

RefSeq Version	XM_005264369.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264369.3

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGAAGC AGATAGCAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( XM_005264369.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264426.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35437
Clone ID Related Accession (Same CDS sequence)	XM_005264369.3 , XM_005264369.2
Accession Version	XM_005264369.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGAAGC AGATAGCAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264426.1
CDS	100..663
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQKQIAR

Target ORF information:

RefSeq Version	XM_005264369.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264369.2

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGAAGC AGATAGCAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( XM_011532908.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531210.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59682
Clone ID Related Accession (Same CDS sequence)	XM_011532908.2 , XM_011532908.3
Accession Version	XM_011532908.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGGGG CTTGGTACTG TGAAATGAGT AACTTAATCC TGACAACCGT AGTGCAAGGT 
ATGGCCCATC TCCTGTACGC TTGGAGCGAC CTTTGGCTAC GTGGCTGGCC TTGTTATTTC 
ACCACTCTGG ATATACTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531210.1
CDS	100..783
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQGAWYCEMSNLILTTVVQGMAHLLYAWSDLWLRGWPCYFTTLDILE

Target ORF information:

RefSeq Version	XM_011532908.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532908.2

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGGGG CTTGGTACTG TGAAATGAGT AACTTAATCC TGACAACCGT AGTGCAAGGT 
ATGGCCCATC TCCTGTACGC TTGGAGCGAC CTTTGGCTAC GTGGCTGGCC TTGTTATTTC 
ACCACTCTGG ATATACTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGF ( XM_011532908.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531210.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59682
Clone ID Related Accession (Same CDS sequence)	XM_011532908.2 , XM_011532908.3
Accession Version	XM_011532908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan biosynthesis class F protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGGGG CTTGGTACTG TGAAATGAGT AACTTAATCC TGACAACCGT AGTGCAAGGT 
ATGGCCCATC TCCTGTACGC TTGGAGCGAC CTTTGGCTAC GTGGCTGGCC TTGTTATTTC 
ACCACTCTGG ATATACTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531210.1
CDS	110..793
Translation	MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQGAWYCEMSNLILTTVVQGMAHLLYAWSDLWLRGWPCYFTTLDILE

Target ORF information:

RefSeq Version	XM_011532908.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532908.3

ORF Insert Sequence:

ATGAAAGATA ACGATATCAA GAGACTACTG TATACCCATC TTTTATGCAT ATTTTCAATT 
ATCCTAAGTG TCTTCATTCC ATCACTCTTC TTGGAGAACT TCTCAATATT GGAAACACAC 
TTGACATGGT TGTGCATCTG TTCTGGTTTT GTAACTGCTG TCAATCTAGT ACTATATTTA 
GTAGTGAAAC CAAATACATC CTCTAAAAGA AGTTCATTAT CACACAAGGT AACTGGATTT 
TTGAAATGCT GTATCTACTT TCTTATGTCT TGTTTCTCCT TTCATGTAAT TTTTGTTCTG 
TATGGAGCAC CACTGATAGA GTTGGCATTG GAAACATTTT TATTTGCAGT TATTTTGTCT 
ACTTTTACTA CTGTGCCTTG CTTATGTTTG TTAGGACCAA ACCTCAAAGC ATGGCTAAGA 
GTGTTCAGTA GAAATGGAGT TACATCCATA TGGGAGAATA GTCTCCAGAT CACTACAATT 
TCTAGCTTTG TAGGAGCATG GCTTGGAGCA CTTCCTATTC CACTGGATTG GGAAAGACCA 
TGGCAGGGGG CTTGGTACTG TGAAATGAGT AACTTAATCC TGACAACCGT AGTGCAAGGT 
ATGGCCCATC TCCTGTACGC TTGGAGCGAC CTTTGGCTAC GTGGCTGGCC TTGTTATTTC 
ACCACTCTGG ATATACTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhanced HIF2? expression during human trophoblast differentiation into syncytiotrophoblast suppresses transcription of placental growth factor.
Scientific reports7(1)12455(2017 Sep)
Fujii T,Nagamatsu T,Morita K,Schust DJ,Iriyama T,Komatsu A,Osuga Y,Fujii T

New insights into the functions of PIGF, a protein involved in the ethanolamine phosphate transfer steps of glycosylphosphatidylinositol biosynthesis.
The Biochemical journal463(2)249-56(2014 Oct)
Stokes MJ,Murakami Y,Maeda Y,Kinoshita T,Morita YS

Retinal reactive astrocytic tumor: gene expression profiling.
JAMA ophthalmology132(6)773-5(2014 Jun)
Shehri M,Bouhenni R,Ghazi NG,Abu Safieh L,Edward DP

Identification of type 2 diabetes-associated combination of SNPs using support vector machine.
BMC genetics1126(2010 Apr)
Ban HJ,Heo JY,Oh KS,Park KJ

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PIGF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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