ABCB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCB1
Entrez Gene ID	5243
Full Name	ATP binding cassette subfamily B member 1
Synonyms	ABC20,CD243,CLCS,GP170,MDR1,P-GP,PGY1
General protein information	Preferred Names ATP binding cassette subfamily B member 1 Names multidrug resistance protein 1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 P glycoprotein P-glycoprotein 1 colchicin sensitivity doxorubicin resistance
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.12
Summary	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017].
Disorder MIM:	171050
Disorder Html:	{Colchicine resistance}, 120080 (3); {Inflammatory bowel disease 13}, 612244 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001348945.1	NP_001335874.1	ATP-dependent translocase ABCB1 isoform 1
NM_001348946.2	NP_001335875.1	ATP-dependent translocase ABCB1 isoform 2
NM_000927.4	NP_000918.2	ATP-dependent translocase ABCB1 isoform 2
NM_001348944.1	NP_001335873.1	ATP-dependent translocase ABCB1 isoform 2
NM_001348946.1	NP_001335875.1	multidrug resistance protein 1 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ABCB1	XP_001163417.1
Mus musculus (house mouse)	Abcb1a	NP_035206.2
Rattus norvegicus (Norway rat)	Abcb1a	NP_596892.1
Caenorhabditis elegans (roundworm)	pgp-9	NP_507487.1
Arabidopsis thaliana (thale cress)	PGP9	NP_193539.6
	ABCB1	NP_001028059.1
Bos taurus (cattle)	ABCB1	XP_002686777.3
Drosophila melanogaster (fruit fly)	Mdr50	NP_523740.3
Arabidopsis thaliana (thale cress)	PGP5	NP_192092.1
Oryza sativa (rice)	Os01g0695700	NP_001043962.1
Xenopus tropicalis (tropical clawed frog)	abcb1	XP_004921510.1
Caenorhabditis elegans (roundworm)	pgp-1	NP_502413.1
Schizosaccharomyces pombe (fission yeast)	pmd1	NP_588265.1
Arabidopsis thaliana (thale cress)	PGP11	NP_171753.1
Arabidopsis thaliana (thale cress)	PGP7	NP_199466.1
Arabidopsis thaliana (thale cress)	ABCB4	NP_182223.1
Arabidopsis thaliana (thale cress)	PGP12	NP_171754.1
Homo sapiens (human)	ABCB1	NP_000918.2
Canis lupus familiaris (dog)	ABCB1	NP_001003215.1
Magnaporthe oryzae (rice blast fungus)	MGG_00141	XP_003718990.1
Neurospora crassa	NCU07546	XP_959059.2
Arabidopsis thaliana (thale cress)	PGP21	NP_191774.2
Arabidopsis thaliana (thale cress)	PGP3	NP_192091.1

Gene Ontology

The following ABCB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu102681	NM_001348945.1 Latest version!	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu23307	NM_001348946.2 Latest version!	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu23307	NM_000927.4 Latest version!	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu23307	NM_001348944.1 Latest version!	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
View Old Accession Versions >>
OHu23307	NM_001348946.1	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCB1 ( NM_001348945.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335874.1 Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu102681
Clone ID Related Accession (Same CDS sequence)	NM_001348945.1
Accession Version	NM_001348945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	ATP-dependent translocase ABCB1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002457.2 and AC005068.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.69469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTCA ACTTGCAAGG GGACCAGAGA GGTGCAACGG AAGCCAGAAC ATTCCTCCTG 
GAAATTCAAC CTGTTTCGCA GTTTCTCGAG GAATCAGCAT TCAGTCAATC CGGGCCGGGA 
GCAGTCATCT GTGGTCTTTC CACTAAAGTC GGAGTATCTT CTTCCAAAAT TTCACGTCTT 
GGTGGCCGTT CCAAGGAGCG CGAGGTCGGA ATGGATCTTG AAGGGGACCG CAATGGAGGA 
GCAAAGAAGA AGAACTTTTT TAAACTGAAC AATAAAAGTG AAAAAGATAA GAAGGAAAAG 
AAACCAACTG TCAGTGTATT TTCAATGTTT CGCTATTCAA ATTGGCTTGA CAAGTTGTAT 
ATGGTGGTGG GAACTTTGGC TGCCATCATC CATGGGGCTG GACTTCCTCT CATGATGCTG 
GTGTTTGGAG AAATGACAGA TATCTTTGCA AATGCAGGAA ATTTAGAAGA TCTGATGTCA 
AACATCACTA ATAGAAGTGA TATCAATGAT ACAGGGTTCT TCATGAATCT GGAGGAAGAC 
ATGACCAGGT ATGCCTATTA TTACAGTGGA ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC 
ATTCAGGTTT CATTTTGGTG CCTGGCAGCT GGAAGACAAA TACACAAAAT TAGAAAACAG 
TTTTTTCATG CTATAATGCG ACAGGAGATA GGCTGGTTTG ATGTGCACGA TGTTGGGGAG 
CTTAACACCC GACTTACAGA TGATGTCTCC AAGATTAATG AAGGAATTGG TGACAAAATT 
GGAATGTTCT TTCAGTCAAT GGCAACATTT TTCACTGGGT TTATAGTAGG ATTTACACGT 
GGTTGGAAGC TAACCCTTGT GATTTTGGCC ATCAGTCCTG TTCTTGGACT GTCAGCTGCT 
GTCTGGGCAA AGATACTATC TTCATTTACT GATAAAGAAC TCTTAGCGTA TGCAAAAGCT 
GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA ATTAGAACTG TGATTGCATT TGGAGGACAA 
AAGAAAGAAC TTGAAAGGTA CAACAAAAAT TTAGAAGAAG CTAAAAGAAT TGGGATAAAG 
AAAGCTATTA CAGCCAATAT TTCTATAGGT GCTGCTTTCC TGCTGATCTA TGCATCTTAT 
GCTCTGGCCT TCTGGTATGG GACCACCTTG GTCCTCTCAG GGGAATATTC TATTGGACAA 
GTACTCACTG TATTCTTTTC TGTATTAATT GGGGCTTTTA GTGTTGGACA GGCATCTCCA 
AGCATTGAAG CATTTGCAAA TGCAAGAGGA GCAGCTTATG AAATCTTCAA GATAATTGAT 
AATAAGCCAA GTATTGACAG CTATTCGAAG AGTGGGCACA AACCAGATAA TATTAAGGGA 
AATTTGGAAT TCAGAAATGT TCACTTCAGT TACCCATCTC GAAAAGAAGT TAAGATCTTG 
AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG CAGACGGTGG CCCTGGTTGG AAACAGTGGC 
TGTGGGAAGA GCACAACAGT CCAGCTGATG CAGAGGCTCT ATGACCCCAC AGAGGGGATG 
GTCAGTGTTG ATGGACAGGA TATTAGGACC ATAAATGTAA GGTTTCTACG GGAAATCATT 
GGTGTGGTGA GTCAGGAACC TGTATTGTTT GCCACCACGA TAGCTGAAAA CATTCGCTAT 
GGCCGTGAAA ATGTCACCAT GGATGAGATT GAGAAAGCTG TCAAGGAAGC CAATGCCTAT 
GACTTTATCA TGAAACTGCC TCATAAATTT GACACCCTGG TTGGAGAGAG AGGGGCCCAG 
TTGAGTGGTG GGCAGAAGCA GAGGATCGCC ATTGCACGTG CCCTGGTTCG CAACCCCAAG 
ATCCTCCTGC TGGATGAGGC CACGTCAGCC TTGGACACAG AAAGCGAAGC AGTGGTTCAG 
GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG ACCACCATTG TGATAGCTCA TCGTTTGTCT 
ACAGTTCGTA ATGCTGACGT CATCGCTGGT TTCGATGATG GAGTCATTGT GGAGAAAGGA 
AATCATGATG AACTCATGAA AGAGAAAGGC ATTTACTTCA AACTTGTCAC AATGCAGACA 
GCAGGAAATG AAGTTGAATT AGAAAATGCA GCTGATGAAT CCAAAAGTGA AATTGATGCC 
TTGGAAATGT CTTCAAATGA TTCAAGATCC AGTCTAATAA GAAAAAGATC AACTCGTAGG 
AGTGTCCGTG GATCACAAGC CCAAGACAGA AAGCTTAGTA CCAAAGAGGC TCTGGATGAA 
AGTATACCTC CAGTTTCCTT TTGGAGGATT ATGAAGCTAA ATTTAACTGA ATGGCCTTAT 
TTTGTTGTTG GTGTATTTTG TGCCATTATA AATGGAGGCC TGCAACCAGC ATTTGCAATA 
ATATTTTCAA AGATTATAGG GGTTTTTACA AGAATTGATG ATCCTGAAAC AAAACGACAG 
AATAGTAACT TGTTTTCACT ATTGTTTCTA GCCCTTGGAA TTATTTCTTT TATTACATTT 
TTCCTTCAGG GTTTCACATT TGGCAAAGCT GGAGAGATCC TCACCAAGCG GCTCCGATAC 
ATGGTTTTCC GATCCATGCT CAGACAGGAT GTGAGTTGGT TTGATGACCC TAAAAACACC 
ACTGGAGCAT TGACTACCAG GCTCGCCAAT GATGCTGCTC AAGTTAAAGG GGCTATAGGT 
TCCAGGCTTG CTGTAATTAC CCAGAATATA GCAAATCTTG GGACAGGAAT AATTATATCC 
TTCATCTATG GTTGGCAACT AACACTGTTA CTCTTAGCAA TTGTACCCAT CATTGCAATA 
GCAGGAGTTG TTGAAATGAA AATGTTGTCT GGACAAGCAC TGAAAGATAA GAAAGAACTA 
GAAGGTTCTG GGAAGATCGC TACTGAAGCA ATAGAAAACT TCCGAACCGT TGTTTCTTTG 
ACTCAGGAGC AGAAGTTTGA ACATATGTAT GCTCAGAGTT TGCAGGTACC ATACAGAAAC 
TCTTTGAGGA AAGCACACAT CTTTGGAATT ACATTTTCCT TCACCCAGGC AATGATGTAT 
TTTTCCTATG CTGGATGTTT CCGGTTTGGA GCCTACTTGG TGGCACATAA ACTCATGAGC 
TTTGAGGATG TTCTGTTAGT ATTTTCAGCT GTTGTCTTTG GTGCCATGGC CGTGGGGCAA 
GTCAGTTCAT TTGCTCCTGA CTATGCCAAA GCCAAAATAT CAGCAGCCCA CATCATCATG 
ATCATTGAAA AAACCCCTTT GATTGACAGC TACAGCACGG AAGGCCTAAT GCCGAACACA 
TTGGAAGGAA ATGTCACATT TGGTGAAGTT GTATTCAACT ATCCCACCCG ACCGGACATC 
CCAGTGCTTC AGGGACTGAG CCTGGAGGTG AAGAAGGGCC AGACGCTGGC TCTGGTGGGC 
AGCAGTGGCT GTGGGAAGAG CACAGTGGTC CAGCTCCTGG AGCGGTTCTA CGACCCCTTG 
GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA ATAAAGCGAC TGAATGTTCA GTGGCTCCGA 
GCACACCTGG GCATCGTGTC CCAGGAGCCC ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC 
ATTGCCTATG GAGACAACAG CCGGGTGGTG TCACAGGAAG AGATTGTGAG GGCAGCAAAG 
GAGGCCAACA TACATGCCTT CATCGAGTCA CTGCCTAATA AATATAGCAC TAAAGTAGGA 
GACAAAGGAA CTCAGCTCTC TGGTGGCCAG AAACAACGCA TTGCCATAGC TCGTGCCCTT 
GTTAGACAGC CTCATATTTT GCTTTTGGAT GAAGCCACGT CAGCTCTGGA TACAGAAAGT 
GAAAAGGTTG TCCAAGAAGC CCTGGACAAA GCCAGAGAAG GCCGCACCTG CATTGTGATT 
GCTCACCGCC TGTCCACCAT CCAGAATGCA GACTTAATAG TGGTGTTTCA GAATGGCAGA 
GTCAAGGAGC ATGGCACGCA TCAGCAGCTG CTGGCACAGA AAGGCATCTA TTTTTCAATG 
GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335874.1
CDS	336..4388
Translation	MSVNLQGDQRGATEARTFLLEIQPVSQFLEESAFSQSGPGAVICGLSTKVGVSSSKISRLGGRSKEREVGMDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Target ORF information:

RefSeq Version	NM_001348945.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348945.1

ORF Insert Sequence:

ATGAGTGTCA ACTTGCAAGG GGACCAGAGA GGTGCAACGG AAGCCAGAAC ATTCCTCCTG 
GAAATTCAAC CTGTTTCGCA GTTTCTCGAG GAATCAGCAT TCAGTCAATC CGGGCCGGGA 
GCAGTCATCT GTGGTCTTTC CACTAAAGTC GGAGTATCTT CTTCCAAAAT TTCACGTCTT 
GGTGGCCGTT CCAAGGAGCG CGAGGTCGGA ATGGATCTTG AAGGGGACCG CAATGGAGGA 
GCAAAGAAGA AGAACTTTTT TAAACTGAAC AATAAAAGTG AAAAAGATAA GAAGGAAAAG 
AAACCAACTG TCAGTGTATT TTCAATGTTT CGCTATTCAA ATTGGCTTGA CAAGTTGTAT 
ATGGTGGTGG GAACTTTGGC TGCCATCATC CATGGGGCTG GACTTCCTCT CATGATGCTG 
GTGTTTGGAG AAATGACAGA TATCTTTGCA AATGCAGGAA ATTTAGAAGA TCTGATGTCA 
AACATCACTA ATAGAAGTGA TATCAATGAT ACAGGGTTCT TCATGAATCT GGAGGAAGAC 
ATGACCAGGT ATGCCTATTA TTACAGTGGA ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC 
ATTCAGGTTT CATTTTGGTG CCTGGCAGCT GGAAGACAAA TACACAAAAT TAGAAAACAG 
TTTTTTCATG CTATAATGCG ACAGGAGATA GGCTGGTTTG ATGTGCACGA TGTTGGGGAG 
CTTAACACCC GACTTACAGA TGATGTCTCC AAGATTAATG AAGGAATTGG TGACAAAATT 
GGAATGTTCT TTCAGTCAAT GGCAACATTT TTCACTGGGT TTATAGTAGG ATTTACACGT 
GGTTGGAAGC TAACCCTTGT GATTTTGGCC ATCAGTCCTG TTCTTGGACT GTCAGCTGCT 
GTCTGGGCAA AGATACTATC TTCATTTACT GATAAAGAAC TCTTAGCGTA TGCAAAAGCT 
GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA ATTAGAACTG TGATTGCATT TGGAGGACAA 
AAGAAAGAAC TTGAAAGGTA CAACAAAAAT TTAGAAGAAG CTAAAAGAAT TGGGATAAAG 
AAAGCTATTA CAGCCAATAT TTCTATAGGT GCTGCTTTCC TGCTGATCTA TGCATCTTAT 
GCTCTGGCCT TCTGGTATGG GACCACCTTG GTCCTCTCAG GGGAATATTC TATTGGACAA 
GTACTCACTG TATTCTTTTC TGTATTAATT GGGGCTTTTA GTGTTGGACA GGCATCTCCA 
AGCATTGAAG CATTTGCAAA TGCAAGAGGA GCAGCTTATG AAATCTTCAA GATAATTGAT 
AATAAGCCAA GTATTGACAG CTATTCGAAG AGTGGGCACA AACCAGATAA TATTAAGGGA 
AATTTGGAAT TCAGAAATGT TCACTTCAGT TACCCATCTC GAAAAGAAGT TAAGATCTTG 
AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG CAGACGGTGG CCCTGGTTGG AAACAGTGGC 
TGTGGGAAGA GCACAACAGT CCAGCTGATG CAGAGGCTCT ATGACCCCAC AGAGGGGATG 
GTCAGTGTTG ATGGACAGGA TATTAGGACC ATAAATGTAA GGTTTCTACG GGAAATCATT 
GGTGTGGTGA GTCAGGAACC TGTATTGTTT GCCACCACGA TAGCTGAAAA CATTCGCTAT 
GGCCGTGAAA ATGTCACCAT GGATGAGATT GAGAAAGCTG TCAAGGAAGC CAATGCCTAT 
GACTTTATCA TGAAACTGCC TCATAAATTT GACACCCTGG TTGGAGAGAG AGGGGCCCAG 
TTGAGTGGTG GGCAGAAGCA GAGGATCGCC ATTGCACGTG CCCTGGTTCG CAACCCCAAG 
ATCCTCCTGC TGGATGAGGC CACGTCAGCC TTGGACACAG AAAGCGAAGC AGTGGTTCAG 
GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG ACCACCATTG TGATAGCTCA TCGTTTGTCT 
ACAGTTCGTA ATGCTGACGT CATCGCTGGT TTCGATGATG GAGTCATTGT GGAGAAAGGA 
AATCATGATG AACTCATGAA AGAGAAAGGC ATTTACTTCA AACTTGTCAC AATGCAGACA 
GCAGGAAATG AAGTTGAATT AGAAAATGCA GCTGATGAAT CCAAAAGTGA AATTGATGCC 
TTGGAAATGT CTTCAAATGA TTCAAGATCC AGTCTAATAA GAAAAAGATC AACTCGTAGG 
AGTGTCCGTG GATCACAAGC CCAAGACAGA AAGCTTAGTA CCAAAGAGGC TCTGGATGAA 
AGTATACCTC CAGTTTCCTT TTGGAGGATT ATGAAGCTAA ATTTAACTGA ATGGCCTTAT 
TTTGTTGTTG GTGTATTTTG TGCCATTATA AATGGAGGCC TGCAACCAGC ATTTGCAATA 
ATATTTTCAA AGATTATAGG GGTTTTTACA AGAATTGATG ATCCTGAAAC AAAACGACAG 
AATAGTAACT TGTTTTCACT ATTGTTTCTA GCCCTTGGAA TTATTTCTTT TATTACATTT 
TTCCTTCAGG GTTTCACATT TGGCAAAGCT GGAGAGATCC TCACCAAGCG GCTCCGATAC 
ATGGTTTTCC GATCCATGCT CAGACAGGAT GTGAGTTGGT TTGATGACCC TAAAAACACC 
ACTGGAGCAT TGACTACCAG GCTCGCCAAT GATGCTGCTC AAGTTAAAGG GGCTATAGGT 
TCCAGGCTTG CTGTAATTAC CCAGAATATA GCAAATCTTG GGACAGGAAT AATTATATCC 
TTCATCTATG GTTGGCAACT AACACTGTTA CTCTTAGCAA TTGTACCCAT CATTGCAATA 
GCAGGAGTTG TTGAAATGAA AATGTTGTCT GGACAAGCAC TGAAAGATAA GAAAGAACTA 
GAAGGTTCTG GGAAGATCGC TACTGAAGCA ATAGAAAACT TCCGAACCGT TGTTTCTTTG 
ACTCAGGAGC AGAAGTTTGA ACATATGTAT GCTCAGAGTT TGCAGGTACC ATACAGAAAC 
TCTTTGAGGA AAGCACACAT CTTTGGAATT ACATTTTCCT TCACCCAGGC AATGATGTAT 
TTTTCCTATG CTGGATGTTT CCGGTTTGGA GCCTACTTGG TGGCACATAA ACTCATGAGC 
TTTGAGGATG TTCTGTTAGT ATTTTCAGCT GTTGTCTTTG GTGCCATGGC CGTGGGGCAA 
GTCAGTTCAT TTGCTCCTGA CTATGCCAAA GCCAAAATAT CAGCAGCCCA CATCATCATG 
ATCATTGAAA AAACCCCTTT GATTGACAGC TACAGCACGG AAGGCCTAAT GCCGAACACA 
TTGGAAGGAA ATGTCACATT TGGTGAAGTT GTATTCAACT ATCCCACCCG ACCGGACATC 
CCAGTGCTTC AGGGACTGAG CCTGGAGGTG AAGAAGGGCC AGACGCTGGC TCTGGTGGGC 
AGCAGTGGCT GTGGGAAGAG CACAGTGGTC CAGCTCCTGG AGCGGTTCTA CGACCCCTTG 
GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA ATAAAGCGAC TGAATGTTCA GTGGCTCCGA 
GCACACCTGG GCATCGTGTC CCAGGAGCCC ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC 
ATTGCCTATG GAGACAACAG CCGGGTGGTG TCACAGGAAG AGATTGTGAG GGCAGCAAAG 
GAGGCCAACA TACATGCCTT CATCGAGTCA CTGCCTAATA AATATAGCAC TAAAGTAGGA 
GACAAAGGAA CTCAGCTCTC TGGTGGCCAG AAACAACGCA TTGCCATAGC TCGTGCCCTT 
GTTAGACAGC CTCATATTTT GCTTTTGGAT GAAGCCACGT CAGCTCTGGA TACAGAAAGT 
GAAAAGGTTG TCCAAGAAGC CCTGGACAAA GCCAGAGAAG GCCGCACCTG CATTGTGATT 
GCTCACCGCC TGTCCACCAT CCAGAATGCA GACTTAATAG TGGTGTTTCA GAATGGCAGA 
GTCAAGGAGC ATGGCACGCA TCAGCAGCTG CTGGCACAGA AAGGCATCTA TTTTTCAATG 
GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( NM_001348946.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335875.1 Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu23307
Clone ID Related Accession (Same CDS sequence)	NM_001348946.2 , NM_000927.4 , NM_001348944.1 , NM_001348946.1
Accession Version	NM_001348946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	ATP-dependent translocase ABCB1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130424.1, AY425005.1, EU852583.1, AB208970.1 and AC005068.2. On Jul 1, 2019 this sequence version replaced NM_001348946.1. Transcript Variant: This variant (4) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Variants 2, 3, and 4 all encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335875.1
CDS	137..3979
Translation	MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Target ORF information:

RefSeq Version	NM_001348946.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348946.2

ORF Insert Sequence:

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( NM_000927.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000918.2 Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 3, mRNA.

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CloneID	OHu23307
Clone ID Related Accession (Same CDS sequence)	NM_001348946.2 , NM_000927.4 , NM_001348944.1 , NM_001348946.1
Accession Version	NM_000927.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	ATP-dependent translocase ABCB1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290159.1, AC002457.2 and AA776371.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2011 this sequence version replaced NM_000927.3. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Variants 2, 3, and 4 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290159.1, SRR1660809.208280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000918.2
CDS	494..4336
Translation	MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Target ORF information:

RefSeq Version	NM_000927.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000927.4

ORF Insert Sequence:

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( NM_001348944.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335873.1 Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 2, mRNA.

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CloneID	OHu23307
Clone ID Related Accession (Same CDS sequence)	NM_001348946.2 , NM_000927.4 , NM_001348944.1 , NM_001348946.1
Accession Version	NM_001348944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	ATP-dependent translocase ABCB1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002457.2 and AC005068.2. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Variants 2, 3, and 4 all encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.200562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335873.1
CDS	347..4189
Translation	MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Target ORF information:

RefSeq Version	NM_001348944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348944.1

ORF Insert Sequence:

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCB1 ( NM_001348946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335875.1 Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.

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CloneID	OHu23307
Clone ID Related Accession (Same CDS sequence)	NM_001348946.2 , NM_000927.4 , NM_001348944.1 , NM_001348946.1
Accession Version	NM_001348946.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	multidrug resistance protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002457.2 and AC005068.2. Transcript Variant: This variant (4) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Variants 2, 3, and 4 all encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC130424.1, SRR1803613.111013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335875.1
CDS	141..3983
Translation	MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Target ORF information:

RefSeq Version	NM_001348946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348946.1

ORF Insert Sequence:

ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC 
AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT 
CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC 
CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA 
AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT 
ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA 
ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT 
GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA 
GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC 
AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT 
TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC 
ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT 
GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA 
ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT 
TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT 
GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG 
GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT 
GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA 
GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG 
AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT 
TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG 
CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG 
CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC 
ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT 
GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT 
GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT 
GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC 
ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC 
TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG 
ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT 
TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC 
ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA 
GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC 
AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA 
AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT 
ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA 
AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA 
AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA 
GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT 
GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT 
GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT 
GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA 
GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA 
CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT 
GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA 
ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT 
GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT 
ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA 
GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT 
GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA 
GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC 
TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT 
GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG 
AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC 
CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA 
ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG 
TCACAGGAAG AGATTGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA 
CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG 
AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT 
GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA 
GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA 
GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG 
CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ABCB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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