PEPD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEPD
Entrez Gene ID	5184
Full Name	peptidase D
Synonyms	PROLIDASE
General protein information	Preferred Names peptidase D Names xaa-Pro dipeptidase X-Pro dipeptidase aminoacyl-L-proline hydrolase imidodipeptidase proline dipeptidase testicular tissue protein Li 138
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.11
Summary	This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].
Disorder MIM:	613230
Disorder Html:	Prolidase deficiency, 170100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166057.2	NP_001159529.1	xaa-Pro dipeptidase isoform 3
NM_000285.3	NP_000276.2	xaa-Pro dipeptidase isoform 1
NM_001166056.2	NP_001159528.1	xaa-Pro dipeptidase isoform 2
NM_001166057.1	NP_001159529.1	xaa-Pro dipeptidase isoform 3
NM_001166056.1	NP_001159528.1	xaa-Pro dipeptidase isoform 2
NM_000285.4	NP_000276.2	xaa-Pro dipeptidase isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PEPD	XP_512574.3
Bos taurus (cattle)	PEPD	NP_001073787.2
Drosophila melanogaster (fruit fly)	Dip-C	NP_650192.1
Rattus norvegicus (Norway rat)	Pepd	NP_001009641.1
Gallus gallus (chicken)	PEPD	NP_001073185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004775	XP_318039.3
Oryza sativa (rice)	Os02g0224400	NP_001046335.1
Caenorhabditis elegans (roundworm)	K12C11.1	NP_490843.1
	PEPD	XP_001108725.2
Mus musculus (house mouse)	Pepd	NP_032846.2
Danio rerio (zebrafish)	pepd	NP_944594.1
Arabidopsis thaliana (thale cress)	AT4G29490	NP_194678.2
Xenopus tropicalis (tropical clawed frog)	pepd	NP_001008094.1
Homo sapiens (human)	PEPD	NP_000276.2
Canis lupus familiaris (dog)	PEPD	XP_533702.2

Gene Ontology
Bibliography

Related articles in PubMed

PEPD is a pivotal regulator of p53 tumor suppressor.
Yang L, Li Y, Bhattacharya A, Zhang Y
Nature communications8(1)2052(2017 Dec)

Serum prolidase enzyme activity in obese subjects and its relationship with oxidative stress markers.
Aslan M, Duzenli U, Esen R, Soyoral YU
Clinica chimica acta; international journal of clinical chemistry473186-190(2017 Oct)

Substrate specificity and reaction mechanism of human prolidase.
Wilk P, Uehlein M, Kalms J, Dobbek H, Mueller U, Weiss MS
The FEBS journal284(17)2870-2885(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High resolution X-ray crystallographic models of substrate and product binding in the active site of human prolidase.
Title: Substrate specificity and reaction mechanism of human prolidase.

Prolidase activity decreases in patients with chronic obstructive pulmonary disease.
Title: The relationships among the levels of oxidative and antioxidative parameters, FEV1 and prolidase activity in COPD.

We report here that by introducing either the A252R or P365R substitution mutation, the structural changes affecting catalytic turnover rate and substrate binding affinity are valuable in improving the catalytic activity of human prolidase towards toxic organophosphorus compound hydrolysis.
Title: Improved Hydrolysis of Organophosphorus Compounds by Engineered Human Prolidases.

In ankylosing spondylitis, serum prolidase level was successful in measuring disease activity
Title: Serum prolidase level in ankylosing spondylitis: low serum levels as a new potential gold standard biomarker for disease activity.

there is an independent relationship of aortic prolidase activity and aortic prolidase immunostaining with aortic stiffness beta index in patients who underwent coronary artery bypass grafting
Title: Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

The following PEPD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEPD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24790	NM_000285.4 Latest version!	Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24752	NM_001166056.2 Latest version!	Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu25079	NM_001166057.2 Latest version!	Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
View Old Accession Versions >>
OHu25079	NM_001166057.1	Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OHu24752	NM_001166056.1	Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu24790	NM_000285.3	Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEPD ( NM_000285.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000276.2 Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24790
Clone ID Related Accession (Same CDS sequence)	NM_000285.3 , NM_000285.4
Accession Version	NM_000285.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, BC015027.1 and AW512991.1. This sequence is a reference standard in the RefSeqGene project. On Oct 6, 2009 this sequence version replaced NM_000285.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005451.1, BC015027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA 
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT 
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC 
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA 
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG 
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA 
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG 
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG 
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG 
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA 
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA 
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC 
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG 
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC 
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA 
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000276.2
CDS	134..1615
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_000285.3
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000285.3

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA 
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT 
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC 
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA 
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG 
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA 
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG 
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG 
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG 
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA 
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA 
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC 
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG 
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC 
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA 
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEPD ( NM_000285.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000276.2 Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24790
Clone ID Related Accession (Same CDS sequence)	NM_000285.3 , NM_000285.4
Accession Version	NM_000285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015027.1 and AW512991.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000285.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.640554.1, SRR3476690.130374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000244137.12/ ENSP00000244137.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA 
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT 
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC 
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA 
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG 
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA 
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG 
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG 
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG 
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA 
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA 
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC 
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG 
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC 
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA 
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000276.2
CDS	32..1513
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_000285.4
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000285.4

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA 
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT 
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC 
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA 
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG 
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA 
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG 
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG 
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG 
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA 
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA 
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC 
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG 
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC 
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA 
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEPD ( NM_001166056.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159528.1 Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24752
Clone ID Related Accession (Same CDS sequence)	NM_001166056.2 , NM_001166056.1
Accession Version	NM_001166056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294619.1 and AW512991.1. On May 31, 2019 this sequence version replaced NM_001166056.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294619.1, SRR3476690.953951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC 
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT 
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT 
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC 
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG 
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG 
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC 
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC 
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG 
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC 
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG 
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC 
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC 
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159528.1
CDS	32..1390
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_001166056.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166056.2

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC 
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT 
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT 
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC 
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG 
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG 
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC 
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC 
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG 
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC 
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG 
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC 
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC 
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEPD ( NM_001166056.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159528.1 Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24752
Clone ID Related Accession (Same CDS sequence)	NM_001166056.2 , NM_001166056.1
Accession Version	NM_001166056.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK294619.1 and AW512991.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1064819.1, SRR3476690.953951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC 
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT 
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT 
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC 
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG 
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG 
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC 
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC 
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG 
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC 
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG 
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC 
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC 
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159528.1
CDS	134..1492
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_001166056.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166056.1

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA 
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT 
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG 
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG 
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG 
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC 
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC 
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT 
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT 
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC 
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG 
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG 
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC 
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC 
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG 
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC 
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG 
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC 
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC 
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEPD ( NM_001166057.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159529.1 Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu25079
Clone ID Related Accession (Same CDS sequence)	NM_001166057.2 , NM_001166057.1
Accession Version	NM_001166057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK293126.1, BC015027.1 and AW512991.1. On May 31, 2019 this sequence version replaced NM_001166057.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC 
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC 
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA 
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG 
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC 
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC 
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC 
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC 
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC 
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC 
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG 
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG 
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG 
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG 
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG 
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC 
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG 
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG 
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159529.1
CDS	32..1321
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_001166057.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166057.2

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC 
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC 
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA 
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG 
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC 
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC 
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC 
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC 
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC 
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC 
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG 
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG 
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG 
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG 
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG 
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC 
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG 
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG 
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEPD ( NM_001166057.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159529.1 Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu25079
Clone ID Related Accession (Same CDS sequence)	NM_001166057.2 , NM_001166057.1
Accession Version	NM_001166057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	xaa-Pro dipeptidase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK293126.1, BC015027.1 and AW512991.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC 
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC 
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA 
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG 
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC 
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC 
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC 
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC 
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC 
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC 
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG 
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG 
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG 
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG 
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG 
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC 
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG 
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG 
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159529.1
CDS	134..1423
Translation	MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Target ORF information:

RefSeq Version	NM_001166057.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166057.1

ORF Insert Sequence:

ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG 
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC 
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC 
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC 
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA 
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG 
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC 
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC 
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC 
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC 
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC 
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC 
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG 
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG 
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG 
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG 
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG 
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC 
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG 
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG 
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEPD is a pivotal regulator of p53 tumor suppressor.
Nature communications8(1)2052(2017 Dec)
Yang L,Li Y,Bhattacharya A,Zhang Y

Serum prolidase enzyme activity in obese subjects and its relationship with oxidative stress markers.
Clinica chimica acta; international journal of clinical chemistry473186-190(2017 Oct)
Aslan M,Duzenli U,Esen R,Soyoral YU

Substrate specificity and reaction mechanism of human prolidase.
The FEBS journal284(17)2870-2885(2017 Sep)
Wilk P,Uehlein M,Kalms J,Dobbek H,Mueller U,Weiss MS

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PEPD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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