POLR3K cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR3K
Entrez Gene ID	51728
Full Name	RNA polymerase III subunit K
Synonyms	C11,C11-RNP3,My010,RPC10,RPC11,RPC12.5
General protein information	Preferred Names RNA polymerase III subunit K Names DNA-directed RNA polymerase III subunit RPC10 DNA-directed RNA polymerase III subunit K DNA-directed RNA polymerases III 12.5 kDa polypeptide RNA polymerase III 12.5 kDa subunit RNA polymerase III subunit C10 RNA polymerase III subunit C11 RNA polymerase III subunit CII polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa polymerase (RNA) III subunit K
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010].
Disorder MIM:	606007

mRNA and Protein(s)

mRNA	Protein	Name
NM_016310.4	NP_057394.3	DNA-directed RNA polymerase III subunit RPC10
NM_016310.5	NP_057394.3	DNA-directed RNA polymerase III subunit RPC10

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polr3k	NP_080177.1
Danio rerio (zebrafish)	polr3k	NP_001002553.1
Xenopus tropicalis (tropical clawed frog)	polr3k	NP_001015803.1
Pan troglodytes (chimpanzee)	POLR3K	XP_001150299.1
Kluyveromyces lactis	KLLA0F18568g	XP_455912.1
Saccharomyces cerevisiae (baker's yeast)	RPC11	NP_010330.1
Bos taurus (cattle)	POLR3K	NP_001040082.1
Rattus norvegicus (Norway rat)	Polr3k	NP_001014281.1
Drosophila melanogaster (fruit fly)	CG33785	NP_001027443.1
Schizosaccharomyces pombe (fission yeast)	rpc11	NP_593235.1
Magnaporthe oryzae (rice blast fungus)	MGG_03072	XP_003720603.1
Neurospora crassa	NCU00570	XP_964731.1
Homo sapiens (human)	POLR3K	NP_057394.2
Caenorhabditis elegans (roundworm)	Y77E11A.6	NP_500076.1
Eremothecium gossypii	AGOS_AGR029W	NP_986694.1

Gene Ontology
Bibliography

Related articles in PubMed

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrent? L, Mar JC
PLoS genetics11(8)e1005428(2015 Aug)

Genome-wide association analysis of red blood cell traits in African Americans: the COGENT Network.
Chen Z, Tang H, Qayyum R, Schick UM, Nalls MA, Handsaker R, Li J, Lu Y, Yanek LR, Keating B, Meng Y, van Rooij FJ, Okada Y, Kubo M, Rasmussen-Torvik L, Keller MF, Lange L, Evans M, Bottinger EP, Linderman MD, Ruderfer DM, Hakonarson H, Papanicolaou G, Zonderman AB, Gottesman O, , , Thomson C, Ziv E, Singleton AB, Loos RJ, Sleiman PM, Ganesh S, McCarroll S, Becker DM, Wilson JG, Lettre G, Reiner AP
Human molecular genetics22(12)2529-38(2013 Jun)

Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases.
Johnson SS, Zhang C, Fromm J, Willis IM, Johnson DL
Molecular cell26(3)367-79(2007 May)

Pan-genome isolation of low abundance transcripts using SAGE tag.
Kim YC, Jung YC, Xuan Z, Dong H, Zhang MQ, Wang SM
FEBS letters580(28-29)6721-9(2006 Dec)

A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter.
De Gobbi M, Viprakasit V, Hughes JR, Fisher C, Buckle VJ, Ayyub H, Gibbons RJ, Vernimmen D, Yoshinaga Y, de Jong P, Cheng JF, Rubin EM, Wood WG, Bowden D, Higgs DR
Science (New York, N.Y.)312(5777)1215-7(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Changes in Maf1 expression affect Pol III-dependent transcription in human glioblastoma lines.
Title: Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases.

The following POLR3K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR3K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23621	NM_016310.5 Latest version!	Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23621	NM_016310.4	Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR3K ( NM_016310.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057394.3 Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.

Price & Availability↑

CloneID	OHu23621
Clone ID Related Accession (Same CDS sequence)	NM_016310.4 , NM_016310.5
Accession Version	NM_016310.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase III subunit RPC10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z69719.1, KF456133.1, BC011932.2 and DR978472.1. On Mar 25, 2014 this sequence version replaced NM_016310.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.24399.1, SRR1660803.249233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGT TCTGCCCCGG CTGCGGGAAC GGGCTGATCG TGGAGGAGGG ACAACGCTGC 
CACCGCTTCG CCTGCAACAC GTGCCCCTAC GTGCACAACA TCACCCGCAA GGTAACAAAT 
CGGAAGTACC CAAAACTGAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTTGACT CTACTGCAGA GTCGTGTCCC AAATGCGAAC ATCCTCGTGC TTACTTCATG 
CAGCTTCAGA CCCGCTCTGC AGATGAGCCG ATGACCACCT TCTACAAGTG CTGCAATGCT 
CAGTGTGGAC ACCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057394.3
CDS	47..373
Translation	MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

Target ORF information:

RefSeq Version	NM_016310.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016310.4

ORF Insert Sequence:

ATGCTGCTGT TCTGCCCCGG CTGCGGGAAC GGGCTGATCG TGGAGGAGGG ACAACGCTGC 
CACCGCTTCG CCTGCAACAC GTGCCCCTAC GTGCACAACA TCACCCGCAA GGTAACAAAT 
CGGAAGTACC CAAAACTGAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTTGACT CTACTGCAGA GTCGTGTCCC AAATGCGAAC ATCCTCGTGC TTACTTCATG 
CAGCTTCAGA CCCGCTCTGC AGATGAGCCG ATGACCACCT TCTACAAGTG CTGCAATGCT 
CAGTGTGGAC ACCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3K ( NM_016310.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057394.3 Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.

Price & Availability↑

CloneID	OHu23621
Clone ID Related Accession (Same CDS sequence)	NM_016310.4 , NM_016310.5
Accession Version	NM_016310.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase III subunit RPC10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF456133.1, BC011932.2, DR978472.1 and Z69719.1. On May 17, 2019 this sequence version replaced NM_016310.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF060223.1, SRR1803613.29839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293860.6/ ENSP00000293860.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGT TCTGCCCCGG CTGCGGGAAC GGGCTGATCG TGGAGGAGGG ACAACGCTGC 
CACCGCTTCG CCTGCAACAC GTGCCCCTAC GTGCACAACA TCACCCGCAA GGTAACAAAT 
CGGAAGTACC CAAAACTGAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTTGACT CTACTGCAGA GTCGTGTCCC AAATGCGAAC ATCCTCGTGC TTACTTCATG 
CAGCTTCAGA CCCGCTCTGC AGATGAGCCG ATGACCACCT TCTACAAGTG CTGCAATGCT 
CAGTGTGGAC ACCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057394.3
CDS	23..349
Translation	MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

Target ORF information:

RefSeq Version	NM_016310.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase III subunit K (POLR3K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016310.5

ORF Insert Sequence:

ATGCTGCTGT TCTGCCCCGG CTGCGGGAAC GGGCTGATCG TGGAGGAGGG ACAACGCTGC 
CACCGCTTCG CCTGCAACAC GTGCCCCTAC GTGCACAACA TCACCCGCAA GGTAACAAAT 
CGGAAGTACC CAAAACTGAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTTGACT CTACTGCAGA GTCGTGTCCC AAATGCGAAC ATCCTCGTGC TTACTTCATG 
CAGCTTCAGA CCCGCTCTGC AGATGAGCCG ATGACCACCT TCTACAAGTG CTGCAATGCT 
CAGTGTGGAC ACCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
PLoS genetics11(8)e1005428(2015 Aug)
Hasegawa Y,Taylor D,Ovchinnikov DA,Wolvetang EJ,de Torrent? L,Mar JC

Genome-wide association analysis of red blood cell traits in African Americans: the COGENT Network.
Human molecular genetics22(12)2529-38(2013 Jun)
Chen Z,Tang H,Qayyum R,Schick UM,Nalls MA,Handsaker R,Li J,Lu Y,Yanek LR,Keating B,Meng Y,van Rooij FJ,Okada Y,Kubo M,Rasmussen-Torvik L,Keller MF,Lange L,Evans M,Bottinger EP,Linderman MD,Ruderfer DM,Hakonarson H,Papanicolaou G,Zonderman AB,Gottesman O,,,Thomson C,Ziv E,Singleton AB,Loos RJ,Sleiman PM,Ganesh S,McCarroll S,Becker DM,Wilson JG,Lettre G,Reiner AP

Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases.
Molecular cell26(3)367-79(2007 May)
Johnson SS,Zhang C,Fromm J,Willis IM,Johnson DL

Pan-genome isolation of low abundance transcripts using SAGE tag.
FEBS letters580(28-29)6721-9(2006 Dec)
Kim YC,Jung YC,Xuan Z,Dong H,Zhang MQ,Wang SM

A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter.
Science (New York, N.Y.)312(5777)1215-7(2006 May)
De Gobbi M,Viprakasit V,Hughes JR,Fisher C,Buckle VJ,Ayyub H,Gibbons RJ,Vernimmen D,Yoshinaga Y,de Jong P,Cheng JF,Rubin EM,Wood WG,Bowden D,Higgs DR

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POLR3K cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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