ORF » Species Summary » Homo sapiens » NLK cDNA ORF clone

NLK cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following NLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu50459 XM_005257988.2
Latest version!		 Homo sapiens nemo like kinase (NLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu13346 NM_016231.5
Latest version!		 Homo sapiens nemo like kinase (NLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
View Old Accession Versions >>
OHu13346 NM_016231.4 Homo sapiens nemo like kinase (NLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NLK ( XM_005257988.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258045.1 Homo sapiens nemo like kinase (NLK), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu50459
    Clone ID Related Accession (Same CDS sequence) XM_005257988.2
    Accession Version XM_005257988.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase NLK isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005257988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGT CCAAAAGAAT ATCCGCTAAG GATGCCTTAG CCCACCCCTA CCTAGATGAA 
    GGGCGACTAC GATATCACAC ATGTATGTGT AAATGTTGCT TTTCCACCTC CACTGGAAGA 
    GTTTATACCA GTGACTTTGA GCCTGTCACC AATCCCAAAT TTGATGACAC TTTCGAGAAG 
    AACCTCAGTT CTGTCCGACA GGTTAAAGAA ATTATTCATC AGTTCATTTT GGAACAGCAG 
    AAAGGAAACA GAGTGCCTCT CTGCATCAAC CCTCAGTCTG CTGCTTTTAA GAGCTTTATT 
    AGTTCCACTG TTGCTCAGCC ATCTGAGATG CCCCCATCTC CTCTGGTGTG GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258045.1
    CDS228..1724
    Translation

    Target ORF information:

    RefSeq Version XM_005257988.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nemo like kinase (NLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005257988.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGT CCAAAAGAAT ATCCGCTAAG GATGCCTTAG CCCACCCCTA CCTAGATGAA 
    GGGCGACTAC GATATCACAC ATGTATGTGT AAATGTTGCT TTTCCACCTC CACTGGAAGA 
    GTTTATACCA GTGACTTTGA GCCTGTCACC AATCCCAAAT TTGATGACAC TTTCGAGAAG 
    AACCTCAGTT CTGTCCGACA GGTTAAAGAA ATTATTCATC AGTTCATTTT GGAACAGCAG 
    AAAGGAAACA GAGTGCCTCT CTGCATCAAC CCTCAGTCTG CTGCTTTTAA GAGCTTTATT 
    AGTTCCACTG TTGCTCAGCC ATCTGAGATG CCCCCATCTC CTCTGGTGTG GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NLK ( NM_016231.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057315.3 Homo sapiens nemo like kinase (NLK), mRNA.
    Price & Availability↑
    CloneID OHu13346
    Clone ID Related Accession (Same CDS sequence) NM_016231.4 , NM_016231.5
    Accession Version NM_016231.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase NLK
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090287.9, DA224552.1, AK024686.1, U69562.1, DA573077.1, BQ636970.1, BM723604.1 and AL036279.2. On Jun 22, 2007 this sequence version replaced NM_016231.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064663.1, SRR1803617.172398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGC CATCTCTTCC TGTACTCTAT ACCCTGTCTA GCCAGGCTAC ACATGAAGCT 
    GTTCATCTCC TTTGCAGGAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
    GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGACTACGAT ATCACACATG TATGTGTAAA 
    TGTTGCTTTT CCACCTCCAC TGGAAGAGTT TATACCAGTG ACTTTGAGCC TGTCACCAAT 
    CCCAAATTTG ATGACACTTT CGAGAAGAAC CTCAGTTCTG TCCGACAGGT TAAAGAAATT 
    ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
    CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACTGTTG CTCAGCCATC TGAGATGCCC 
    CCATCTCCTC TGGTGTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057315.3
    CDS213..1796
    Translation

    Target ORF information:

    RefSeq Version NM_016231.4
    Organism Homo sapiens(human)
    Definition Homo sapiens nemo like kinase (NLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_016231.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGC CATCTCTTCC TGTACTCTAT ACCCTGTCTA GCCAGGCTAC ACATGAAGCT 
    GTTCATCTCC TTTGCAGGAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
    GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGACTACGAT ATCACACATG TATGTGTAAA 
    TGTTGCTTTT CCACCTCCAC TGGAAGAGTT TATACCAGTG ACTTTGAGCC TGTCACCAAT 
    CCCAAATTTG ATGACACTTT CGAGAAGAAC CTCAGTTCTG TCCGACAGGT TAAAGAAATT 
    ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
    CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACTGTTG CTCAGCCATC TGAGATGCCC 
    CCATCTCCTC TGGTGTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NLK ( NM_016231.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057315.3 Homo sapiens nemo like kinase (NLK), mRNA.
    Price & Availability↑
    CloneID OHu13346
    Clone ID Related Accession (Same CDS sequence) NM_016231.4 , NM_016231.5
    Accession Version NM_016231.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase NLK
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090287.9, DA224552.1, AK024686.1, U69562.1, DA573077.1, BQ636970.1, BM723604.1 and AL036279.2. On Nov 22, 2018 this sequence version replaced NM_016231.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064663.1, SRR1803617.172398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000407008.8/ ENSP00000384625.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGC CATCTCTTCC TGTACTCTAT ACCCTGTCTA GCCAGGCTAC ACATGAAGCT 
    GTTCATCTCC TTTGCAGGAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
    GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGACTACGAT ATCACACATG TATGTGTAAA 
    TGTTGCTTTT CCACCTCCAC TGGAAGAGTT TATACCAGTG ACTTTGAGCC TGTCACCAAT 
    CCCAAATTTG ATGACACTTT CGAGAAGAAC CTCAGTTCTG TCCGACAGGT TAAAGAAATT 
    ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
    CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACTGTTG CTCAGCCATC TGAGATGCCC 
    CCATCTCCTC TGGTGTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057315.3
    CDS198..1781
    Translation

    Target ORF information:

    RefSeq Version NM_016231.5
    Organism Homo sapiens(human)
    Definition Homo sapiens nemo like kinase (NLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_016231.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCTTT GTGGCGCAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
    TCTGCAGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCACA CCTCCCTCCT 
    CCTCACCTGC ACCACCACCA CCACCCTCAA CACCATCTTC ATCCGGGGTC GGCTGCCGCT 
    GTACACCCTG TACAGCAGCA CACCTCTTCG GCAGCTGCGG CAGCCGCAGC AGCGGCTGCA 
    GCTGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCACC GGCACCGGGG 
    CAGGCTCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AGGCTGCCGC AGCTGCTACA 
    GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCACAGC AGCAGCTGGA TATTGAGCCG 
    GATAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
    GGAAAGAGAG TAGCGCTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
    AGGGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAGC ATGATAATGT ACTCTCTGCC 
    CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
    TTGATGCAGA GTGACCTACA TAAAATTATC GTCTCTCCTC AACCACTCAG CTCAGATCAT 
    GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
    TTACATCGAG ACATTAAGCC AGGGAATCTC CTTGTGAACA GCAACTGTGT TCTAAAGATT 
    TGTGATTTTG GATTGGCCAG AGTGGAAGAA TTAGATGAAT CCCGTCATAT GACTCAGGAA 
    GTTGTTACTC AGTATTATCG GGCTCCAGAA ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
    GCTATTGACA TCTGGTCTGT GGGATGTATC TTTGCAGAAC TACTAGGACG AAGAATATTG 
    TTTCAGGCAC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CGGATCTGTT GGGCACACCA 
    TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGCGCTAAGG CACATATACT CAGGGGTCCT 
    CATAAACAGC CATCTCTTCC TGTACTCTAT ACCCTGTCTA GCCAGGCTAC ACATGAAGCT 
    GTTCATCTCC TTTGCAGGAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
    GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGACTACGAT ATCACACATG TATGTGTAAA 
    TGTTGCTTTT CCACCTCCAC TGGAAGAGTT TATACCAGTG ACTTTGAGCC TGTCACCAAT 
    CCCAAATTTG ATGACACTTT CGAGAAGAAC CTCAGTTCTG TCCGACAGGT TAAAGAAATT 
    ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
    CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACTGTTG CTCAGCCATC TGAGATGCCC 
    CCATCTCCTC TGGTGTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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