CHMP3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHMP3
Entrez Gene ID	51652
Full Name	charged multivesicular body protein 3
Synonyms	CGI-149,NEDF,VPS24
General protein information	Preferred Names charged multivesicular body protein 3 Names charged multivesicular body protein 3 25.1 protein CHMP family, member 3 chromatin-modifying protein 3 neuroendocrine differentiation factor vacuolar protein sorting 24 homolog vacuolar protein sorting-associated protein 24
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p11.2
Summary	This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010].
Disorder MIM:	610052

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005753.3	NP_001005753.1	charged multivesicular body protein 3 isoform 2
NM_001193517.2	NP_001180446.1	charged multivesicular body protein 3 isoform 3
NM_016079.3	NP_057163.1	charged multivesicular body protein 3 isoform 1
NM_001193517.1	NP_001180446.1	charged multivesicular body protein 3 isoform 3
NM_001005753.2	NP_001005753.1	charged multivesicular body protein 3 isoform 2
NM_016079.4	NP_057163.1	charged multivesicular body protein 3 isoform 1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	VPS24	NP_012883.1
Neurospora crassa	NCU01791	XP_956469.1
Pan troglodytes (chimpanzee)	VPS24	XP_001138427.1
Mus musculus (house mouse)	Chmp3	NP_080059.2
Rattus norvegicus (Norway rat)	Chmp3	NP_758834.1
Gallus gallus (chicken)	CHMP3	XP_420858.2
Danio rerio (zebrafish)	chmp3	NP_998485.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003507	XP_309652.2
Oryza sativa (rice)	Os03g0108400	NP_001048700.2
Eremothecium gossypii	AGOS_ABR049C	NP_982995.2
Magnaporthe oryzae (rice blast fungus)	MGG_06670	XP_003709375.1
Arabidopsis thaliana (thale cress)	VPS24.1	NP_197686.1
Homo sapiens (human)	CHMP3	NP_057163.1
Caenorhabditis elegans (roundworm)	T27F7.1	NP_494919.1
Kluyveromyces lactis	KLLA0F25366g	XP_456210.1
Xenopus tropicalis (tropical clawed frog)	chmp3	NP_001016229.2
Bos taurus (cattle)	CHMP3	NP_001030223.2
Drosophila melanogaster (fruit fly)	vps24	NP_649451.1
Arabidopsis thaliana (thale cress)	VPS24.2	NP_190086.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
Cashikar AG, Shim S, Roth R, Maldazys MR, Heuser JE, Hanson PI
eLife3(2014 May)

Helical structures of ESCRT-III are disassembled by VPS4.
Lata S, Schoehn G, Jain A, Pires R, Piehler J, Gottlinger HG, Weissenhorn W
Science (New York, N.Y.)321(5894)1354-7(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein kinase CK2 alpha is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly.
Title: CK2 involvement in ESCRT-III complex phosphorylation.

tight coupling of ESCRT-III CHMP3 and AMSH functions and provide insight into the regulation of ESCRT-III
Title: Structural basis for ESCRT-III CHMP3 recruitment of AMSH.

Data show that the N-terminal core domains of increased sodium tolerance-1 (IST1) and charged multivesicular body protein-3 (CHMP3) form equivalent four-helix bundles, revealing that IST1 is a previously unrecognized ESCRT-III family member.
Title: Structural basis for ESCRT-III protein autoinhibition.

study found the ESCRT-III proteins CHMP2A & CHMP3 could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule; VPS4 could bind on the inside of the tubule & disassemble the tubes
Title: Helical structures of ESCRT-III are disassembled by VPS4.

UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7.
Title: The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation.

The following CHMP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHMP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17608	NM_016079.4 Latest version!	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu23234	NM_001005753.3 Latest version!	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22047	NM_001193517.2 Latest version!	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu22047	NM_001193517.1	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu23234	NM_001005753.2	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17608	NM_016079.3	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHMP3 ( NM_016079.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057163.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17608
Clone ID Related Accession (Same CDS sequence)	NM_016079.3 , NM_016079.4
Accession Version	NM_016079.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK312353.1, BC004419.1, BU192410.1, AK025046.1 and AF151907.1. On Jul 30, 2010 this sequence version replaced NM_016079.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Vps24alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF151907.1, SRR1660809.80551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCTG TGAGCAAGCT GTATGCATCC 
AAAGCACACA TGAACTCAGT GCTCATGGGG ATGAAGAACC AGCTCGCGGT CTTGCGAGTG 
GCTGGTTCCC TGCAGAAGAG CACAGAAGTG ATGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057163.1
CDS	150..818
Translation	MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_016079.3
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016079.3

ORF Insert Sequence:

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCTG TGAGCAAGCT GTATGCATCC 
AAAGCACACA TGAACTCAGT GCTCATGGGG ATGAAGAACC AGCTCGCGGT CTTGCGAGTG 
GCTGGTTCCC TGCAGAAGAG CACAGAAGTG ATGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP3 ( NM_016079.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057163.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17608
Clone ID Related Accession (Same CDS sequence)	NM_016079.3 , NM_016079.4
Accession Version	NM_016079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK312353.1, BC004419.1, BU192410.1, AK025046.1 and AF151907.1. On Nov 23, 2018 this sequence version replaced NM_016079.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Vps24alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF151907.1, SRR1660809.80551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263856.9/ ENSP00000263856.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCTG TGAGCAAGCT GTATGCATCC 
AAAGCACACA TGAACTCAGT GCTCATGGGG ATGAAGAACC AGCTCGCGGT CTTGCGAGTG 
GCTGGTTCCC TGCAGAAGAG CACAGAAGTG ATGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057163.1
CDS	96..764
Translation	MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_016079.4
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016079.4

ORF Insert Sequence:

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCTG TGAGCAAGCT GTATGCATCC 
AAAGCACACA TGAACTCAGT GCTCATGGGG ATGAAGAACC AGCTCGCGGT CTTGCGAGTG 
GCTGGTTCCC TGCAGAAGAG CACAGAAGTG ATGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP3 ( NM_001005753.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005753.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23234
Clone ID Related Accession (Same CDS sequence)	NM_001005753.3 , NM_001005753.2
Accession Version	NM_001005753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK290725.1, AC068288.6 and AF151907.1. On May 31, 2019 this sequence version replaced NM_001005753.2. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. This results in translation initiation from a downstream start codon and an isoform (2, also known as Vps24beta) that has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.158794.1, SRR3476690.1025478.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGGT CAAGGAAGGC TGTGAGCAAG CTGTATGCAT CCAAAGCACA CATGAACTCA 
GTGCTCATGG GGATGAAGAA CCAGCTCGCG GTCTTGCGAG TGGCTGGTTC CCTGCAGAAG 
AGCACAGAAG TGATGAAGGC CATGCAAAGT CTTGTGAAGA TTCCAGAGAT TCAGGCCACC 
ATGAGGGAGT TGTCCAAAGA AATGATGAAG GCTGGGATCA TAGAGGAGAT GTTAGAGGAC 
ACTTTTGAAA GCATGGACGA TCAGGAAGAA ATGGAGGAAG AAGCAGAAAT GGAAATTGAC 
AGAATTCTCT TTGAAATTAC AGCAGGGGCC TTGGGCAAAG CACCCAGTAA AGTGACTGAT 
GCCCTTCCAG AGCCAGAACC TCCAGGAGCG ATGGCTGCCT CAGAGGATGA GGAGGAGGAG 
GAAGAGGCTC TGGAGGCCAT GCAGTCCCGG CTGGCCACAC TCCGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005753.1
CDS	233..703
Translation	MIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_001005753.3
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005753.3

ORF Insert Sequence:

ATGATCAGGT CAAGGAAGGC TGTGAGCAAG CTGTATGCAT CCAAAGCACA CATGAACTCA 
GTGCTCATGG GGATGAAGAA CCAGCTCGCG GTCTTGCGAG TGGCTGGTTC CCTGCAGAAG 
AGCACAGAAG TGATGAAGGC CATGCAAAGT CTTGTGAAGA TTCCAGAGAT TCAGGCCACC 
ATGAGGGAGT TGTCCAAAGA AATGATGAAG GCTGGGATCA TAGAGGAGAT GTTAGAGGAC 
ACTTTTGAAA GCATGGACGA TCAGGAAGAA ATGGAGGAAG AAGCAGAAAT GGAAATTGAC 
AGAATTCTCT TTGAAATTAC AGCAGGGGCC TTGGGCAAAG CACCCAGTAA AGTGACTGAT 
GCCCTTCCAG AGCCAGAACC TCCAGGAGCG ATGGCTGCCT CAGAGGATGA GGAGGAGGAG 
GAAGAGGCTC TGGAGGCCAT GCAGTCCCGG CTGGCCACAC TCCGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP3 ( NM_001005753.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005753.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23234
Clone ID Related Accession (Same CDS sequence)	NM_001005753.3 , NM_001005753.2
Accession Version	NM_001005753.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK290725.1, AC068288.6 and AF151907.1. On Jul 30, 2010 this sequence version replaced NM_001005753.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. This results in translation initiation from a downstream start codon and an isoform (2, also known as Vps24beta) that has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.154695.1, SRR1803616.252186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGGT CAAGGAAGGC TGTGAGCAAG CTGTATGCAT CCAAAGCACA CATGAACTCA 
GTGCTCATGG GGATGAAGAA CCAGCTCGCG GTCTTGCGAG TGGCTGGTTC CCTGCAGAAG 
AGCACAGAAG TGATGAAGGC CATGCAAAGT CTTGTGAAGA TTCCAGAGAT TCAGGCCACC 
ATGAGGGAGT TGTCCAAAGA AATGATGAAG GCTGGGATCA TAGAGGAGAT GTTAGAGGAC 
ACTTTTGAAA GCATGGACGA TCAGGAAGAA ATGGAGGAAG AAGCAGAAAT GGAAATTGAC 
AGAATTCTCT TTGAAATTAC AGCAGGGGCC TTGGGCAAAG CACCCAGTAA AGTGACTGAT 
GCCCTTCCAG AGCCAGAACC TCCAGGAGCG ATGGCTGCCT CAGAGGATGA GGAGGAGGAG 
GAAGAGGCTC TGGAGGCCAT GCAGTCCCGG CTGGCCACAC TCCGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005753.1
CDS	287..757
Translation	MIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_001005753.2
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005753.2

ORF Insert Sequence:

ATGATCAGGT CAAGGAAGGC TGTGAGCAAG CTGTATGCAT CCAAAGCACA CATGAACTCA 
GTGCTCATGG GGATGAAGAA CCAGCTCGCG GTCTTGCGAG TGGCTGGTTC CCTGCAGAAG 
AGCACAGAAG TGATGAAGGC CATGCAAAGT CTTGTGAAGA TTCCAGAGAT TCAGGCCACC 
ATGAGGGAGT TGTCCAAAGA AATGATGAAG GCTGGGATCA TAGAGGAGAT GTTAGAGGAC 
ACTTTTGAAA GCATGGACGA TCAGGAAGAA ATGGAGGAAG AAGCAGAAAT GGAAATTGAC 
AGAATTCTCT TTGAAATTAC AGCAGGGGCC TTGGGCAAAG CACCCAGTAA AGTGACTGAT 
GCCCTTCCAG AGCCAGAACC TCCAGGAGCG ATGGCTGCCT CAGAGGATGA GGAGGAGGAG 
GAAGAGGCTC TGGAGGCCAT GCAGTCCCGG CTGGCCACAC TCCGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP3 ( NM_001193517.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180446.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.

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CloneID	OHu22047
Clone ID Related Accession (Same CDS sequence)	NM_001193517.2 , NM_001193517.1
Accession Version	NM_001193517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK129502.1, AC068288.6 and AF151907.1. On May 31, 2019 this sequence version replaced NM_001193517.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129502.1, BP226582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180446.1
CDS	96..644
Translation	MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_001193517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193517.2

ORF Insert Sequence:

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP3 ( NM_001193517.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180446.1 Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22047
Clone ID Related Accession (Same CDS sequence)	NM_001193517.2 , NM_001193517.1
Accession Version	NM_001193517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290886.1, AK129502.1, AC068288.6 and AF151907.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129502.1, BP226582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180446.1
CDS	150..698
Translation	MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Target ORF information:

RefSeq Version	NM_001193517.1
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 3 (CHMP3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193517.1

ORF Insert Sequence:

ATGGGGCTGT TTGGAAAGAC CCAGGAGAAG CCGCCCAAAG AACTGGTCAA TGAGTGGTCA 
TTGAAGATAA GAAAGGAAAT GAGAGTTGTT GACAGGCAAA TAAGGGATAT CCAAAGAGAA 
GAAGAAAAAG TGAAACGATC TGTGAAAGAT GCTGCCAAGA AGGGCCAGAA GGATGTCTGC 
ATAGTTCTGG CCAAGGAGAT GATCAGGTCA AGGAAGGCCA TGCAAAGTCT TGTGAAGATT 
CCAGAGATTC AGGCCACCAT GAGGGAGTTG TCCAAAGAAA TGATGAAGGC TGGGATCATA 
GAGGAGATGT TAGAGGACAC TTTTGAAAGC ATGGACGATC AGGAAGAAAT GGAGGAAGAA 
GCAGAAATGG AAATTGACAG AATTCTCTTT GAAATTACAG CAGGGGCCTT GGGCAAAGCA 
CCCAGTAAAG TGACTGATGC CCTTCCAGAG CCAGAACCTC CAGGAGCGAT GGCTGCCTCA 
GAGGATGAGG AGGAGGAGGA AGAGGCTCTG GAGGCCATGC AGTCCCGGCT GGCCACACTC 
CGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
eLife3(2014 May)
Cashikar AG,Shim S,Roth R,Maldazys MR,Heuser JE,Hanson PI

Helical structures of ESCRT-III are disassembled by VPS4.
Science (New York, N.Y.)321(5894)1354-7(2008 Sep)
Lata S,Schoehn G,Jain A,Pires R,Piehler J,Gottlinger HG,Weissenhorn W

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CHMP3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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