PPIL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPIL1
Entrez Gene ID	51645
Full Name	peptidylprolyl isomerase like 1
Synonyms	CGI-124,CYPL1,PPIase,hCyPX
General protein information	Preferred Names peptidylprolyl isomerase like 1 Names peptidyl-prolyl cis-trans isomerase-like 1 cyclophilin like 1 cyclophilin-related gene 1 peptidyl-prolyl cis-trans isomerase peptidylprolyl isomerase (cyclophilin)-like 1 rotamase PPIL1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.2
Summary	This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq, Jul 2008].
Disorder MIM:	601301

mRNA and Protein(s)

mRNA	Protein	Name
NM_016059.4	NP_057143.1	peptidyl-prolyl cis-trans isomerase-like 1
NM_016059.5	NP_057143.1	peptidyl-prolyl cis-trans isomerase-like 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPIL1	NP_001233365.1
Danio rerio (zebrafish)	ppil1	NP_001029350.1
Bos taurus (cattle)	PPIL1	NP_001014869.1
Schizosaccharomyces pombe (fission yeast)	cyp1	NP_593308.1
Arabidopsis thaliana (thale cress)	AT2G36130	NP_181157.1
Xenopus tropicalis (tropical clawed frog)	ppil1	NP_001005007.1
	PPIL1	XP_001096296.2
Canis lupus familiaris (dog)	PPIL1	XP_532126.2
Mus musculus (house mouse)	Ppil1	NP_081121.1
Caenorhabditis elegans (roundworm)	cyn-12	NP_501118.1
Homo sapiens (human)	PPIL1	NP_057143.1
Rattus norvegicus (Norway rat)	Ppil1	NP_001029360.1
Gallus gallus (chicken)	LOC100859332	XP_004951036.1
Neurospora crassa	NCU00578	XP_964739.2
Oryza sativa (rice)	Os08g0559400	NP_001062502.1
Drosophila melanogaster (fruit fly)	Cypl	NP_523874.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012376	XP_001238262.1
Magnaporthe oryzae (rice blast fungus)	MGG_02997	XP_003720695.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

Dynamic protein-protein interaction wiring of the human spliceosome.
Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, L?hrmann R, Stelzl U
Molecular cell45(4)567-80(2012 Feb)

Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases.
Davis TL, Walker JR, Campagna-Slater V, Finerty PJ, Paramanathan R, Bernstein G, MacKenzie F, Tempel W, Ouyang H, Lee WH, Eisenmesser EZ, Dhe-Paganon S
PLoS biology8(7)e1000439(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

molecular model for the binding mode of SKIP to PPIL1 which emphasizes the versatility of cyclophilin-type PPIases to engage in additional interactions with other proteins apart from active site contacts despite their limited surface area.
Title: The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP protein.

The large disordered region in SKIP provides an interaction platform. Its disorder-order transition, induced by PPIL1 binding, may adapt the requirement for a large structural rearrangement occurred in the activation of spliceosome
Title: A large intrinsically disordered region in SKIP and its disorder-order transition induced by PPIL1 binding revealed by NMR.

here the solution structure of PPIL1 determined by NMR spectroscopy
Title: Solution structure of human peptidyl prolyl isomerase-like protein 1 and insights into its interaction with SKIP.

NMR protein structure of PPIL1
Title: Backbone and side chain assignments of human Peptidylprolyl Isomerase Like 1 (hPPIL1).

The following PPIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10401	NM_016059.5 Latest version!	Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10401	NM_016059.4	Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPIL1 ( NM_016059.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057143.1 Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.

Price & Availability↑

CloneID	OHu10401
Clone ID Related Accession (Same CDS sequence)	NM_016059.4 , NM_016059.5
Accession Version	NM_016059.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Homo sapiens(human)
Product	peptidyl-prolyl cis-trans isomerase-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU195579.1, AL122034.29 and AK026636.1. On Dec 5, 2008 this sequence version replaced NM_016059.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.562934.1, AY359032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAA TTCCCCCAGA TTCCTGGCAG CCACCCAACG TTTACTTGGA GACCAGCATG 
GGAATCATTG TGCTGGAGCT GTACTGGAAG CATGCTCCAA AGACCTGTAA GAACTTTGCT 
GAGTTGGCTC GTCGAGGTTA CTACAATGGC ACAAAATTCC ACAGAATTAT CAAAGACTTC 
ATGATCCAAG GAGGTGACCC AACAGGGACA GGTCGAGGTG GTGCATCTAT CTATGGCAAA 
CAGTTTGAAG ATGAACTTCA TCCAGACTTG AAATTCACGG GGGCTGGAAT TCTCGCAATG 
GCCAATGCGG GGCCAGATAC CAATGGCAGC CAGTTCTTTG TGACCCTCGC CCCCACCCAG 
TGGCTTGACG GCAAACACAC CATTTTTGGC CGAGTGTGTC AGGGCATAGG AATGGTGAAT 
CGCGTGGGAA TGGTAGAAAC AAACTCCCAG GACCGCCCTG TGGACGACGT GAAGATCATT 
AAGGCATACC CTTCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057143.1
CDS	253..753
Translation	MAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAMANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQDRPVDDVKIIKAYPSG

Target ORF information:

RefSeq Version	NM_016059.4
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016059.4

ORF Insert Sequence:

ATGGCGGCAA TTCCCCCAGA TTCCTGGCAG CCACCCAACG TTTACTTGGA GACCAGCATG 
GGAATCATTG TGCTGGAGCT GTACTGGAAG CATGCTCCAA AGACCTGTAA GAACTTTGCT 
GAGTTGGCTC GTCGAGGTTA CTACAATGGC ACAAAATTCC ACAGAATTAT CAAAGACTTC 
ATGATCCAAG GAGGTGACCC AACAGGGACA GGTCGAGGTG GTGCATCTAT CTATGGCAAA 
CAGTTTGAAG ATGAACTTCA TCCAGACTTG AAATTCACGG GGGCTGGAAT TCTCGCAATG 
GCCAATGCGG GGCCAGATAC CAATGGCAGC CAGTTCTTTG TGACCCTCGC CCCCACCCAG 
TGGCTTGACG GCAAACACAC CATTTTTGGC CGAGTGTGTC AGGGCATAGG AATGGTGAAT 
CGCGTGGGAA TGGTAGAAAC AAACTCCCAG GACCGCCCTG TGGACGACGT GAAGATCATT 
AAGGCATACC CTTCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPIL1 ( NM_016059.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057143.1 Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.

Price & Availability↑

CloneID	OHu10401
Clone ID Related Accession (Same CDS sequence)	NM_016059.4 , NM_016059.5
Accession Version	NM_016059.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	peptidyl-prolyl cis-trans isomerase-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK026636.1. On Nov 23, 2018 this sequence version replaced NM_016059.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003048.1, AK026636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373699.6/ ENSP00000362803.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCAA TTCCCCCAGA TTCCTGGCAG CCACCCAACG TTTACTTGGA GACCAGCATG 
GGAATCATTG TGCTGGAGCT GTACTGGAAG CATGCTCCAA AGACCTGTAA GAACTTTGCT 
GAGTTGGCTC GTCGAGGTTA CTACAATGGC ACAAAATTCC ACAGAATTAT CAAAGACTTC 
ATGATCCAAG GAGGTGACCC AACAGGGACA GGTCGAGGTG GTGCATCTAT CTATGGCAAA 
CAGTTTGAAG ATGAACTTCA TCCAGACTTG AAATTCACGG GGGCTGGAAT TCTCGCAATG 
GCCAATGCGG GGCCAGATAC CAATGGCAGC CAGTTCTTTG TGACCCTCGC CCCCACCCAG 
TGGCTTGACG GCAAACACAC CATTTTTGGC CGAGTGTGTC AGGGCATAGG AATGGTGAAT 
CGCGTGGGAA TGGTAGAAAC AAACTCCCAG GACCGCCCTG TGGACGACGT GAAGATCATT 
AAGGCATACC CTTCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057143.1
CDS	32..532
Translation	MAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAMANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQDRPVDDVKIIKAYPSG

Target ORF information:

RefSeq Version	NM_016059.5
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidylprolyl isomerase like 1 (PPIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016059.5

ORF Insert Sequence:

ATGGCGGCAA TTCCCCCAGA TTCCTGGCAG CCACCCAACG TTTACTTGGA GACCAGCATG 
GGAATCATTG TGCTGGAGCT GTACTGGAAG CATGCTCCAA AGACCTGTAA GAACTTTGCT 
GAGTTGGCTC GTCGAGGTTA CTACAATGGC ACAAAATTCC ACAGAATTAT CAAAGACTTC 
ATGATCCAAG GAGGTGACCC AACAGGGACA GGTCGAGGTG GTGCATCTAT CTATGGCAAA 
CAGTTTGAAG ATGAACTTCA TCCAGACTTG AAATTCACGG GGGCTGGAAT TCTCGCAATG 
GCCAATGCGG GGCCAGATAC CAATGGCAGC CAGTTCTTTG TGACCCTCGC CCCCACCCAG 
TGGCTTGACG GCAAACACAC CATTTTTGGC CGAGTGTGTC AGGGCATAGG AATGGTGAAT 
CGCGTGGGAA TGGTAGAAAC AAACTCCCAG GACCGCCCTG TGGACGACGT GAAGATCATT 
AAGGCATACC CTTCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

Dynamic protein-protein interaction wiring of the human spliceosome.
Molecular cell45(4)567-80(2012 Feb)
Hegele A,Kamburov A,Grossmann A,Sourlis C,Wowro S,Weimann M,Will CL,Pena V,L?hrmann R,Stelzl U

Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases.
PLoS biology8(7)e1000439(2010 Jul)
Davis TL,Walker JR,Campagna-Slater V,Finerty PJ,Paramanathan R,Bernstein G,MacKenzie F,Tempel W,Ouyang H,Lee WH,Eisenmesser EZ,Dhe-Paganon S

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PPIL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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