SF3B6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SF3B6
Entrez Gene ID	51639
Full Name	splicing factor 3b subunit 6
Synonyms	CGI-110,HSPC175,Ht006,P14,SAP14,SAP14a,SF3B14,SF3B14a
General protein information	Preferred Names splicing factor 3b subunit 6 Names splicing factor 3B subunit 6 SF3b 14 kDa subunit pre-mRNA branch site protein p14 spliceosome-associated protein, 14 kDa subunit spliceosome-associated protein, 14-kDa splicing factor 3B, 14 kDa subunit splicing factor 3b, subunit 6, 14kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008].
Disorder MIM:	607835

mRNA and Protein(s)

mRNA	Protein	Name
NM_016047.4	NP_057131.1	splicing factor 3B subunit 6
NM_016047.3	NP_057131.1	splicing factor 3B subunit 6

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0811700	NP_001051674.1
Gallus gallus (chicken)	SF3B14	XP_419986.1
Danio rerio (zebrafish)	sf3b6	NP_001002067.1
Drosophila melanogaster (fruit fly)	CG13298	NP_648037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002780	XP_312143.4
Xenopus tropicalis (tropical clawed frog)	sf3b14	NP_001017249.1
Homo sapiens (human)	SF3B6	NP_057131.1
	SF3B14	XP_001098393.1
Canis lupus familiaris (dog)	SF3B14	XP_532889.1
Mus musculus (house mouse)	0610009D07Rik	NP_079599.1
Caenorhabditis elegans (roundworm)	C50D2.5	NP_493659.3
Arabidopsis thaliana (thale cress)	AT5G12190	NP_196780.1
Pan troglodytes (chimpanzee)	SF3B14	XP_515324.1
Bos taurus (cattle)	SF3B14	NP_001039482.1
Rattus norvegicus (Norway rat)	LOC100365504	NP_001258151.1

Gene Ontology
Bibliography

Related articles in PubMed

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Teng T, Tsai JH, Puyang X, Seiler M, Peng S, Prajapati S, Aird D, Buonamici S, Caleb B, Chan B, Corson L, Feala J, Fekkes P, Gerard B, Karr C, Korpal M, Liu X, T Lowe J, Mizui Y, Palacino J, Park E, Smith PG, Subramanian V, Wu ZJ, Zou J, Yu L, Chicas A, Warmuth M, Larsen N, Zhu P
Nature communications815522(2017 May)

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Cretu C, Schmitzov? J, Ponce-Salvatierra A, Dybkov O, De Laurentiis EI, Sharma K, Will CL, Urlaub H, L?hrmann R, Pena V
Molecular cell64(2)307-319(2016 Oct)

Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.
Perea W, Schroeder KT, Bryant AN, Greenbaum NL
Biochemistry55(4)629-32(2016 Feb)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the p14-RNA interaction screens charges on the backbone of the branch site during spliceosome assembly.
Title: Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity.

determined the high resolution structure of a complex between SF3b14 and a peptide derived from SF3b155 and proposed a model for recognition of the pre-mRNA branch sequence adenosine.
Title: Crystal structure of a core spliceosomal protein interface.

determined the three-dimensional structure of the human SF3b complex by single-particle electron cryomicroscopy at a resolution of less than 10 angstroms, allowing identification of protein domains with known structural folds
Title: Molecular architecture of the multiprotein splicing factor SF3b.

The following SF3B6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SF3B6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25649	NM_016047.4 Latest version!	Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25649	NM_016047.3	Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SF3B6 ( NM_016047.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057131.1 Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.

Price & Availability↑

CloneID	OHu25649
Clone ID Related Accession (Same CDS sequence)	NM_016047.4 , NM_016047.3
Accession Version	NM_016047.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015463.1 and BG200650.1. On Nov 22, 2018 this sequence version replaced NM_016047.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.172119.1, SRR1163657.34312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233468.5/ ENSP00000233468.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGATGC AAGCGGCCAA GAGGGCGAAC ATTCGACTTC CACCTGAAGT AAATCGGATA 
TTGTATATAA GAAATTTGCC ATACAAAATC ACAGCTGAAG AAATGTATGA TATATTTGGG 
AAATATGGAC CTATTCGTCA AATCAGAGTG GGGAACACAC CTGAAACTAG AGGAACAGCT 
TATGTGGTCT ATGAGGACAT CTTTGATGCC AAGAATGCAT GTGATCACCT ATCGGGATTC 
AATGTTTGTA ACAGATACCT TGTGGTTTTG TACTATAATG CCAACAGGGC ATTTCAGAAG 
ATGGACACAA AGAAGAAGGA GGAACAGTTG AAGCTTCTCA AGGAGAAATA TGGCATCAAC 
ACAGATCCAC CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057131.1
CDS	98..475
Translation	MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK

Target ORF information:

RefSeq Version	NM_016047.4
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016047.4

ORF Insert Sequence:

ATGGCGATGC AAGCGGCCAA GAGGGCGAAC ATTCGACTTC CACCTGAAGT AAATCGGATA 
TTGTATATAA GAAATTTGCC ATACAAAATC ACAGCTGAAG AAATGTATGA TATATTTGGG 
AAATATGGAC CTATTCGTCA AATCAGAGTG GGGAACACAC CTGAAACTAG AGGAACAGCT 
TATGTGGTCT ATGAGGACAT CTTTGATGCC AAGAATGCAT GTGATCACCT ATCGGGATTC 
AATGTTTGTA ACAGATACCT TGTGGTTTTG TACTATAATG CCAACAGGGC ATTTCAGAAG 
ATGGACACAA AGAAGAAGGA GGAACAGTTG AAGCTTCTCA AGGAGAAATA TGGCATCAAC 
ACAGATCCAC CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF3B6 ( NM_016047.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057131.1 Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.

Price & Availability↑

CloneID	OHu25649
Clone ID Related Accession (Same CDS sequence)	NM_016047.4 , NM_016047.3
Accession Version	NM_016047.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015463.1 and BG200650.1. On Oct 23, 2004 this sequence version replaced NM_016047.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015463.1, SRR1163658.323032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGATGC AAGCGGCCAA GAGGGCGAAC ATTCGACTTC CACCTGAAGT AAATCGGATA 
TTGTATATAA GAAATTTGCC ATACAAAATC ACAGCTGAAG AAATGTATGA TATATTTGGG 
AAATATGGAC CTATTCGTCA AATCAGAGTG GGGAACACAC CTGAAACTAG AGGAACAGCT 
TATGTGGTCT ATGAGGACAT CTTTGATGCC AAGAATGCAT GTGATCACCT ATCGGGATTC 
AATGTTTGTA ACAGATACCT TGTGGTTTTG TACTATAATG CCAACAGGGC ATTTCAGAAG 
ATGGACACAA AGAAGAAGGA GGAACAGTTG AAGCTTCTCA AGGAGAAATA TGGCATCAAC 
ACAGATCCAC CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057131.1
CDS	216..593
Translation	MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK

Target ORF information:

RefSeq Version	NM_016047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 6 (SF3B6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016047.3

ORF Insert Sequence:

ATGGCGATGC AAGCGGCCAA GAGGGCGAAC ATTCGACTTC CACCTGAAGT AAATCGGATA 
TTGTATATAA GAAATTTGCC ATACAAAATC ACAGCTGAAG AAATGTATGA TATATTTGGG 
AAATATGGAC CTATTCGTCA AATCAGAGTG GGGAACACAC CTGAAACTAG AGGAACAGCT 
TATGTGGTCT ATGAGGACAT CTTTGATGCC AAGAATGCAT GTGATCACCT ATCGGGATTC 
AATGTTTGTA ACAGATACCT TGTGGTTTTG TACTATAATG CCAACAGGGC ATTTCAGAAG 
ATGGACACAA AGAAGAAGGA GGAACAGTTG AAGCTTCTCA AGGAGAAATA TGGCATCAAC 
ACAGATCCAC CAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Nature communications815522(2017 May)
Teng T,Tsai JH,Puyang X,Seiler M,Peng S,Prajapati S,Aird D,Buonamici S,Caleb B,Chan B,Corson L,Feala J,Fekkes P,Gerard B,Karr C,Korpal M,Liu X,T Lowe J,Mizui Y,Palacino J,Park E,Smith PG,Subramanian V,Wu ZJ,Zou J,Yu L,Chicas A,Warmuth M,Larsen N,Zhu P

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Molecular cell64(2)307-319(2016 Oct)
Cretu C,Schmitzov? J,Ponce-Salvatierra A,Dybkov O,De Laurentiis EI,Sharma K,Will CL,Urlaub H,L?hrmann R,Pena V

Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.
Biochemistry55(4)629-32(2016 Feb)
Perea W,Schroeder KT,Bryant AN,Greenbaum NL

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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SF3B6 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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