DPH5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPH5
Entrez Gene ID	51611
Full Name	diphthamide biosynthesis 5
Synonyms	AD-018,CGI-30,HSPC143,NPD015
General protein information	Preferred Names diphthamide biosynthesis 5 Names diphthine methyl ester synthase DPH5 homolog diphthamide biosynthesis methyltransferase diphthine synthase protein x 0011
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p21.2
Summary	This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	611075

mRNA and Protein(s)

mRNA	Protein	Name
XM_005270938.1	XP_005270995.1	diphthine methyl ester synthase isoform X2
XM_005270941.3	XP_005270998.1	diphthine methyl ester synthase isoform X5
NM_015958.2	NP_057042.2	diphthine methyl ester synthase isoform a
NM_001077395.2	NP_001070863.1	diphthine methyl ester synthase isoform b
XM_017001460.2	XP_016856949.1	diphthine methyl ester synthase isoform X1
XM_011541570.3	XP_011539872.1	diphthine methyl ester synthase isoform X3
XM_011541569.1	XP_011539871.1	diphthine methyl ester synthase isoform X3
XM_017001461.1	XP_016856950.1	diphthine methyl ester synthase isoform X4
XM_006710683.3	XP_006710746.1	diphthine methyl ester synthase isoform X5
NM_015958.3	NP_057042.2	diphthine methyl ester synthase isoform a
XM_017001461.2	XP_016856950.1	diphthine methyl ester synthase isoform X3
XM_005270939.4	XP_005270996.1	diphthine methyl ester synthase isoform X4
XM_005270938.2	XP_005270995.1	diphthine methyl ester synthase isoform X2
XM_011541570.2	XP_011539872.1	diphthine methyl ester synthase isoform X4
XM_017001462.1	XP_016856951.1	diphthine methyl ester synthase isoform X6
XM_017001460.1	XP_016856949.1	diphthine methyl ester synthase isoform X1
XM_024447601.1	XP_024303369.1	diphthine methyl ester synthase isoform X2
NM_001077395.1	NP_001070863.1	diphthine methyl ester synthase isoform b
NM_001077394.1	NP_001070862.1	diphthine methyl ester synthase isoform a
XM_024447600.1	XP_024303368.1	diphthine methyl ester synthase isoform X2
XM_005270939.5	XP_005270996.1	diphthine methyl ester synthase isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DPH5	XP_005621916.1
Drosophila melanogaster (fruit fly)	Dph5	NP_524452.4
	DPH5	XP_001109137.1
Bos taurus (cattle)	DPH5	NP_001070289.1
Danio rerio (zebrafish)	dph5	NP_001093555.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004412	XP_313697.4
Homo sapiens (human)	DPH5	NP_001070862.1
Pan troglodytes (chimpanzee)	DPH5	XP_001137561.1
Mus musculus (house mouse)	Dph5	NP_081469.2
Caenorhabditis elegans (roundworm)	B0491.7	NP_496427.1
Saccharomyces cerevisiae (baker's yeast)	DPH5	NP_013273.1
Kluyveromyces lactis	KLLA0F22836g	XP_456098.1
Schizosaccharomyces pombe (fission yeast)	dph5	NP_588441.1
Neurospora crassa	NCU07266	XP_962120.1
Oryza sativa (rice)	Os03g0355800	NP_001050137.1
Gallus gallus (chicken)	DPH5	XP_422306.1
Xenopus tropicalis (tropical clawed frog)	dph5	NP_001005058.1
Rattus norvegicus (Norway rat)	Dph5	NP_001017449.1
Eremothecium gossypii	AGOS_AFR123W	NP_985670.1
Magnaporthe oryzae (rice blast fungus)	MGG_10496	XP_003713401.1
Arabidopsis thaliana (thale cress)	AT4G31790	NP_974655.1

Gene Ontology
Bibliography

Related articles in PubMed

Drug-gene interactions and the search for missing heritability: a cross-sectional pharmacogenomics study of the QT interval.
Avery CL, Sitlani CM, Arking DE, Arnett DK, Bis JC, Boerwinkle E, Buckley BM, Ida Chen YD, de Craen AJ, Eijgelsheim M, Enquobahrie D, Evans DS, Ford I, Garcia ME, Gudnason V, Harris TB, Heckbert SR, Hochner H, Hofman A, Hsueh WC, Isaacs A, Jukema JW, Knekt P, Kors JA, Krijthe BP, Kristiansson K, Laaksonen M, Liu Y, Li X, Macfarlane PW, Newton-Cheh C, Nieminen MS, Oostra BA, Peloso GM, Porthan K, Rice K, Rivadeneira FF, Rotter JI, Salomaa V, Sattar N, Siscovick DS, Slagboom PE, Smith AV, Sotoodehnia N, Stott DJ, Stricker BH, St?rmer T, Trompet S, Uitterlinden AG, van Duijn C, Westendorp RG, Witteman JC, Whitsel EA, Psaty BM
The pharmacogenomics journal14(1)6-13(2014 Feb)

A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene.
Wei H, Bera TK, Wayne AS, Xiang L, Colantonio S, Chertov O, Pastan I
The Journal of biological chemistry288(17)12305-12(2013 Apr)

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.
Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH
Molecular and cellular biology24(21)9487-97(2004 Nov)

Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL
Proceedings of the National Academy of Sciences of the United States of America97(17)9543-8(2000 Aug)

The post-translational trimethylation of diphthamide studied in vitro.
Moehring JM, Moehring TJ
The Journal of biological chemistry263(8)3840-4(1988 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The yeast and Chinese hamster homologs of human DPH5 catalyze the trimethylation step in the biosynthesis of diphthamide
Title: Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.

The following DPH5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPH5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24931	NM_001077394.1 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu24931	NM_015958.3 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu24931	XM_017001460.2 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu24769	NM_001077395.2 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu33501	XM_005270938.2 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33501	XM_024447600.1 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33501	XM_024447601.1 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33502	XM_011541570.3 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu33502	XM_017001461.2 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu33502	XM_005270939.5 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu33503	XM_006710683.3 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu33503	XM_005270941.3 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu68060	XM_011541569.1 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu90110	XM_017001462.1 Latest version!	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu24931	NM_015958.2	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu33502	XM_017001461.1	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu33501	XM_005270938.1	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33502	XM_011541570.2	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu24931	XM_017001460.1	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu24769	NM_001077395.1	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu33502	XM_005270939.4	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPH5 ( NM_015958.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057042.2 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24931
Clone ID Related Accession (Same CDS sequence)	NM_015958.2 , XM_017001460.2 , NM_015958.3 , XM_017001460.1 , NM_001077394.1
Accession Version	NM_015958.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA133565.1, BC053857.1, AK125485.1 and BM808505.1. On Nov 1, 2006 this sequence version replaced NM_015958.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660809.214828.1, SRR1803614.203966.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057042.2
CDS	148..1005
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	NM_015958.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015958.2

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( NM_015958.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057042.2 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24931
Clone ID Related Accession (Same CDS sequence)	NM_015958.2 , XM_017001460.2 , NM_015958.3 , XM_017001460.1 , NM_001077394.1
Accession Version	NM_015958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA133565.1, BC053857.1, AK125485.1 and BM808505.1. On Nov 22, 2018 this sequence version replaced NM_015958.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.538689.1, SRR1660807.20674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370109.8/ ENSP00000359127.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057042.2
CDS	126..983
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	NM_015958.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015958.3

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( NM_001077394.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070862.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24931
Clone ID Related Accession (Same CDS sequence)	NM_015958.2 , XM_017001460.2 , NM_015958.3 , XM_017001460.1 , NM_001077394.1
Accession Version	NM_001077394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK002046.1, BI907367.1, AK125485.1 and BM808505.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.174313.1, SRR1803615.115705.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070862.1
CDS	166..1023
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	NM_001077394.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077394.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_017001460.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856949.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24931
Clone ID Related Accession (Same CDS sequence)	NM_015958.2 , XM_017001460.2 , NM_015958.3 , XM_017001460.1 , NM_001077394.1
Accession Version	XM_017001460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856949.1
CDS	168..1025
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_017001460.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001460.2

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_017001460.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856949.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24931
Clone ID Related Accession (Same CDS sequence)	NM_015958.2 , XM_017001460.2 , NM_015958.3 , XM_017001460.1 , NM_001077394.1
Accession Version	XM_017001460.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856949.1
CDS	168..1025
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_017001460.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001460.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGCAGTTA CCGAGGAGAC ACTTTGTGTT 
GGCTTAGCCA GGGTTGGAGC CGACGACCAG AAAATTGCAG CAGGCACTTT AAGGCAAATG 
TGCACTGTGG ACTTGGGAGA ACCATTGCAT TCCTTGATCA TCACAGGAGG CAGCATACAT 
CCAATGGAGA TGGAGATGCT AAGTCTGTTT TCCATACCAG AAAATAGCTC AGAATCTCAA 
AGCATCAATG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( NM_001077395.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070863.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24769
Clone ID Related Accession (Same CDS sequence)	NM_001077395.2 , NM_001077395.1
Accession Version	NM_001077395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225469.1, AC093157.2, AK125485.1 and BM808505.1. On May 31, 2019 this sequence version replaced NM_001077395.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is one amino acid shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK289351.1, AK225951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070863.1
CDS	126..980
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	NM_001077395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077395.2

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( NM_001077395.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070863.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24769
Clone ID Related Accession (Same CDS sequence)	NM_001077395.2 , NM_001077395.1
Accession Version	NM_001077395.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA133565.1, AK225469.1, AC093157.2, AK125485.1 and BM808505.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is one amino acid shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.68780.1, AK289351.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070863.1
CDS	148..1002
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	NM_001077395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077395.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA GGGAAGGAAG 
ATCTATGAAC CTCCACGGTA TATGAGTGTA AACCAAGCAG CCCAGCAGCT TCTGGAGATT 
GTTCAAAATC AAAGAATACG AGGAGAAGAA CCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_005270938.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270995.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33501
Clone ID Related Accession (Same CDS sequence)	XM_005270938.1 , XM_005270938.2 , XM_024447601.1 , XM_024447600.1
Accession Version	XM_005270938.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270995.1
CDS	148..870
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLINSYRGDTLCWLSQGWSRRPENCSRHFKANVHCGLGRTIAFLDHHRRQHTSNGDGDAKSVFHTRK

Target ORF information:

RefSeq Version	XM_005270938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270938.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_005270938.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270995.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33501
Clone ID Related Accession (Same CDS sequence)	XM_005270938.1 , XM_005270938.2 , XM_024447601.1 , XM_024447600.1
Accession Version	XM_005270938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005270938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270995.1
CDS	148..870
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLINSYRGDTLCWLSQGWSRRPENCSRHFKANVHCGLGRTIAFLDHHRRQHTSNGDGDAKSVFHTRK

Target ORF information:

RefSeq Version	XM_005270938.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270938.2

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_024447601.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303369.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu33501
Clone ID Related Accession (Same CDS sequence)	XM_005270938.1 , XM_005270938.2 , XM_024447601.1 , XM_024447600.1
Accession Version	XM_024447601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303369.1
CDS	168..890
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLINSYRGDTLCWLSQGWSRRPENCSRHFKANVHCGLGRTIAFLDHHRRQHTSNGDGDAKSVFHTRK

Target ORF information:

RefSeq Version	XM_024447601.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447601.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_024447600.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303368.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu33501
Clone ID Related Accession (Same CDS sequence)	XM_005270938.1 , XM_005270938.2 , XM_024447601.1 , XM_024447600.1
Accession Version	XM_024447600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303368.1
CDS	144..866
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLINSYRGDTLCWLSQGWSRRPENCSRHFKANVHCGLGRTIAFLDHHRRQHTSNGDGDAKSVFHTRK

Target ORF information:

RefSeq Version	XM_024447600.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447600.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA CAGTTACCGA 
GGAGACACTT TGTGTTGGCT TAGCCAGGGT TGGAGCCGAC GACCAGAAAA TTGCAGCAGG 
CACTTTAAGG CAAATGTGCA CTGTGGACTT GGGAGAACCA TTGCATTCCT TGATCATCAC 
AGGAGGCAGC ATACATCCAA TGGAGATGGA GATGCTAAGT CTGTTTTCCA TACCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_011541570.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539872.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_011541570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539872.1
CDS	287..805
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_011541570.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541570.3

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_017001461.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856950.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_017001461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856950.1
CDS	294..812
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_017001461.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001461.1

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_017001461.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856950.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_017001461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856950.1
CDS	294..812
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_017001461.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001461.2

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_005270939.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270996.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_005270939.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270939.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270996.1
CDS	295..813
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_005270939.4
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270939.4

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_011541570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539872.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_011541570.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539872.1
CDS	287..805
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_011541570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541570.2

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_005270939.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270996.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu33502
Clone ID Related Accession (Same CDS sequence)	XM_011541570.3 , XM_017001461.1 , XM_017001461.2 , XM_005270939.4 , XM_011541570.2 , XM_005270939.5
Accession Version	XM_005270939.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005270939.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270996.1
CDS	314..832
Translation	MNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_005270939.5
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270939.5

ORF Insert Sequence:

ATGAATGCTG TAGGCTGCTG TGGTTTACAG TTATATAAGT TTGGAGAGAC AGTTTCTATT 
GTTTTTTGGA CAGACACTTG GAGACCAGAA AGCTTCTTTG ACAAAGTGAA GAAGAACAGA 
CAAAATGGCA TGCACACATT ATGTTTACTA GACATCAAAG TAAAGGAGCA GTCTTTGGAA 
AATCTAATCA AGGGAAGGAA GATCTATGAA CCTCCACGGT ATATGAGTGT AAACCAAGCA 
GCCCAGCAGC TTCTGGAGAT TGTTCAAAAT CAAAGAATAC GAGGAGAAGA ACCAGCAGTT 
ACCGAGGAGA CACTTTGTGT TGGCTTAGCC AGGGTTGGAG CCGACGACCA GAAAATTGCA 
GCAGGCACTT TAAGGCAAAT GTGCACTGTG GACTTGGGAG AACCATTGCA TTCCTTGATC 
ATCACAGGAG GCAGCATACA TCCAATGGAG ATGGAGATGC TAAGTCTGTT TTCCATACCA 
GAAAATAGCT CAGAATCTCA AAGCATCAAT GGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_005270941.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270998.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu33503
Clone ID Related Accession (Same CDS sequence)	XM_005270941.3 , XM_006710683.3
Accession Version	XM_005270941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270998.1
CDS	294..788
Translation	MLISVMLHSLWLVIHLDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_005270941.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270941.3

ORF Insert Sequence:

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_006710683.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710746.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu33503
Clone ID Related Accession (Same CDS sequence)	XM_005270941.3 , XM_006710683.3
Accession Version	XM_006710683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710683.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710746.1
CDS	166..660
Translation	MLISVMLHSLWLVIHLDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_006710683.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710683.3

ORF Insert Sequence:

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GCAGTTACCG AGGAGACACT TTGTGTTGGC 
TTAGCCAGGG TTGGAGCCGA CGACCAGAAA ATTGCAGCAG GCACTTTAAG GCAAATGTGC 
ACTGTGGACT TGGGAGAACC ATTGCATTCC TTGATCATCA CAGGAGGCAG CATACATCCA 
ATGGAGATGG AGATGCTAAG TCTGTTTTCC ATACCAGAAA ATAGCTCAGA ATCTCAAAGC 
ATCAATGGAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_011541569.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539871.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu68060
Clone ID Related Accession (Same CDS sequence)	XM_011541569.1
Accession Version	XM_011541569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA TTACCGAGGA 
GACACTTTGT GTTGGCTTAG CCAGGGTTGG AGCCGACGAC CAGAAAATTG CAGCAGGCAC 
TTTAAGGCAA ATGTGCACTG TGGACTTGGG AGAACCATTG CATTCCTTGA TCATCACAGG 
AGGCAGCATA CATCCAATGG AGATGGAGAT GCTAAGTCTG TTTTCCATAC CAGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539871.1
CDS	148..867
Translation	MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLINYRGDTLCWLSQGWSRRPENCSRHFKANVHCGLGRTIAFLDHHRRQHTSNGDGDAKSVFHTRK

Target ORF information:

RefSeq Version	XM_011541569.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541569.1

ORF Insert Sequence:

ATGCTTTATC TCATCGGGTT GGGCCTGGGA GATGCCAAGG ACATCACAGT CAAGGGCCTG 
GAAGTTGTTA GACGCTGCAG TCGAGTGTAT CTGGAAGCCT ACACCTCAGT CCTAACTGTA 
GGGAAGGAAG CCTTGGAAGA GTTTTATGGA AGAAAATTGG TTGTTGCTGA TAGAGAAGAA 
GTGGAACAAG AAGCAGATAA TATTTTAAAG GATGCTGATA TCAGTGATGT TGCATTCCTT 
GTGGTTGGTG ATCCATTTGG GGCCACAACA CACAGTGATC TTGTTCTAAG AGCAACAAAG 
CTGGGAATTC CTTATAGAGT TATTCACAAT GCCTCCATAA TGAATGCTGT AGGCTGCTGT 
GGTTTACAGT TATATAAGTT TGGAGAGACA GTTTCTATTG TTTTTTGGAC AGACACTTGG 
AGACCAGAAA GCTTCTTTGA CAAAGTGAAG AAGAACAGAC AAAATGGCAT GCACACATTA 
TGTTTACTAG ACATCAAAGT AAAGGAGCAG TCTTTGGAAA ATCTAATCAA TTACCGAGGA 
GACACTTTGT GTTGGCTTAG CCAGGGTTGG AGCCGACGAC CAGAAAATTG CAGCAGGCAC 
TTTAAGGCAA ATGTGCACTG TGGACTTGGG AGAACCATTG CATTCCTTGA TCATCACAGG 
AGGCAGCATA CATCCAATGG AGATGGAGAT GCTAAGTCTG TTTTCCATAC CAGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH5 ( XM_017001462.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856951.1 Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu90110
Clone ID Related Accession (Same CDS sequence)	XM_017001462.1
Accession Version	XM_017001462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diphthine methyl ester synthase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GTTACCGAGG AGACACTTTG TGTTGGCTTA 
GCCAGGGTTG GAGCCGACGA CCAGAAAATT GCAGCAGGCA CTTTAAGGCA AATGTGCACT 
GTGGACTTGG GAGAACCATT GCATTCCTTG ATCATCACAG GAGGCAGCAT ACATCCAATG 
GAGATGGAGA TGCTAAGTCT GTTTTCCATA CCAGAAAATA GCTCAGAATC TCAAAGCATC 
AATGGACTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856951.1
CDS	316..807
Translation	MLISVMLHSLWLVIHLDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Target ORF information:

RefSeq Version	XM_017001462.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 5 (DPH5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001462.1

ORF Insert Sequence:

ATGCTGATAT CAGTGATGTT GCATTCCTTG TGGTTGGTGA TCCATTTGGA CACTTGGAGA 
CCAGAAAGCT TCTTTGACAA AGTGAAGAAG AACAGACAAA ATGGCATGCA CACATTATGT 
TTACTAGACA TCAAAGTAAA GGAGCAGTCT TTGGAAAATC TAATCAAGGG AAGGAAGATC 
TATGAACCTC CACGGTATAT GAGTGTAAAC CAAGCAGCCC AGCAGCTTCT GGAGATTGTT 
CAAAATCAAA GAATACGAGG AGAAGAACCA GTTACCGAGG AGACACTTTG TGTTGGCTTA 
GCCAGGGTTG GAGCCGACGA CCAGAAAATT GCAGCAGGCA CTTTAAGGCA AATGTGCACT 
GTGGACTTGG GAGAACCATT GCATTCCTTG ATCATCACAG GAGGCAGCAT ACATCCAATG 
GAGATGGAGA TGCTAAGTCT GTTTTCCATA CCAGAAAATA GCTCAGAATC TCAAAGCATC 
AATGGACTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Drug-gene interactions and the search for missing heritability: a cross-sectional pharmacogenomics study of the QT interval.
The pharmacogenomics journal14(1)6-13(2014 Feb)
Avery CL,Sitlani CM,Arking DE,Arnett DK,Bis JC,Boerwinkle E,Buckley BM,Ida Chen YD,de Craen AJ,Eijgelsheim M,Enquobahrie D,Evans DS,Ford I,Garcia ME,Gudnason V,Harris TB,Heckbert SR,Hochner H,Hofman A,Hsueh WC,Isaacs A,Jukema JW,Knekt P,Kors JA,Krijthe BP,Kristiansson K,Laaksonen M,Liu Y,Li X,Macfarlane PW,Newton-Cheh C,Nieminen MS,Oostra BA,Peloso GM,Porthan K,Rice K,Rivadeneira FF,Rotter JI,Salomaa V,Sattar N,Siscovick DS,Slagboom PE,Smith AV,Sotoodehnia N,Stott DJ,Stricker BH,St?rmer T,Trompet S,Uitterlinden AG,van Duijn C,Westendorp RG,Witteman JC,Whitsel EA,Psaty BM

A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene.
The Journal of biological chemistry288(17)12305-12(2013 Apr)
Wei H,Bera TK,Wayne AS,Xiang L,Colantonio S,Chertov O,Pastan I

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.
Molecular and cellular biology24(21)9487-97(2004 Nov)
Liu S,Milne GT,Kuremsky JG,Fink GR,Leppla SH

Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
Proceedings of the National Academy of Sciences of the United States of America97(17)9543-8(2000 Aug)
Hu RM,Han ZG,Song HD,Peng YD,Huang QH,Ren SX,Gu YJ,Huang CH,Li YB,Jiang CL,Fu G,Zhang QH,Gu BW,Dai M,Mao YF,Gao GF,Rong R,Ye M,Zhou J,Xu SH,Gu J,Shi JX,Jin WR,Zhang CK,Wu TM,Huang GY,Chen Z,Chen MD,Chen JL

The post-translational trimethylation of diphthamide studied in vitro.
The Journal of biological chemistry263(8)3840-4(1988 Mar)
Moehring JM,Moehring TJ

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DPH5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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見積依頼

Eメール

電話

FAX

オンラインフォーム