PDHA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDHA2
Entrez Gene ID	5161
Full Name	pyruvate dehydrogenase E1 alpha 2 subunit
Synonyms	PDHAL
General protein information	Preferred Names pyruvate dehydrogenase E1 alpha 2 subunit Names pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial PDHE1-A type II pyruvate dehydrogenase (lipoamide) alpha 2 pyruvate dehydrogenase alpha 2 pyruvate dehydrogenase, E1-alpha polypeptide, testis specific
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q22.3
Disorder MIM:	179061

mRNA and Protein(s)

mRNA	Protein	Name
NM_005390.4	NP_005381.1	pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial precursor
NM_005390.5	NP_005381.1	pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDHA2	NP_005381.1
Pan troglodytes (chimpanzee)	PDHA2	XP_526637.2
Bos taurus (cattle)	PDHA2	NP_001070539.1
	PDHA2	XP_001097880.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003030	XP_311846.5
Rattus norvegicus (Norway rat)	Pdha1l1	XP_006253546.1
Drosophila melanogaster (fruit fly)	l(1)G0334	NP_572181.4

Gene Ontology
Bibliography

Related articles in PubMed

Pyruvate dehydrogenase complex: mRNA and protein expression patterns of E1? subunit genes in human spermatogenesis.
Pinheiro A, Silva MJ, Gra?a I, Silva J, S? R, Sousa M, Barros A, Tavares de Almeida I, Rivera I
Gene506(1)173-8(2012 Sep)

Demethylation of the coding region triggers the activation of the human testis-specific PDHA2 gene in somatic tissues.
Pinheiro A, Nunes MJ, Milagre I, Rodrigues E, Silva MJ, de Almeida IT, Rivera I
PloS one7(6)e38076(2012)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of the PDHA1 gene was found in all somatic cells, whereas expression of PDHA2 gene was restricted to germ cells. The switch from X-linked to autosomic gene expression occurred in spermatocytes.
Title: Pyruvate dehydrogenase complex: mRNA and protein expression patterns of E1? subunit genes in human spermatogenesis.

Methylation of the PDHA2 coding region can inhibit gene transcription.
Title: Demethylation of the coding region triggers the activation of the human testis-specific PDHA2 gene in somatic tissues.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

it is the full demethylation of the CpG island located in the coding region that seems to play a crucial role upon PDHA2 gene transcription in testis.
Title: Human testis-specific PDHA2 gene: methylation status of a CpG island in the open reading frame correlates with transcriptional activity.

The following PDHA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30250	NM_005390.5 Latest version!	Homo sapiens pyruvate dehydrogenase E1 subunit alpha 2 (PDHA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu30250	NM_005390.4	Homo sapiens pyruvate dehydrogenase E1 alpha 2 subunit (PDHA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDHA2 ( NM_005390.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005381.1 Homo sapiens pyruvate dehydrogenase E1 alpha 2 subunit (PDHA2), mRNA.

Price & Availability↑

CloneID	OHu30250
Clone ID Related Accession (Same CDS sequence)	NM_005390.4 , NM_005390.5
Accession Version	NM_005390.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093828.3 and BC030697.2. On Mar 23, 2007 this sequence version replaced NM_005390.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC030697.2 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGGCCG CCTTCATCTC CCGCGTGTTG AGGCGAGTTG CCCAGAAATC AGCTCGCAGA 
GTGCTGGTGG CATCCCGTAA CTCCTCAAAT GACGCTACAT TTGAAATTAA GAAATGTGAT 
CTTTATCTGT TGGAAGAGGG TCCCCCTGTC ACTACAGTGC TCACTAGGGC GGAGGGGCTT 
AAATACTACA GGATGATGCT GACTGTTCGC CGCATGGAAT TGAAGGCAGA TCAGCTGTAC 
AAACAGAAAT TCATTCGCGG TTTCTGTCAC CTGTGCGATG GTCAGGAAGC TTGTTGCGTG 
GGCCTTGAGG CCGGCATAAA CCCCTCGGAT CACGTCATTA CATCCTATAG GGCTCATGGT 
GTGTGCTATA CTCGGGGACT TTCTGTCCGA TCCATTCTCG CAGAGCTGAC GGGAAGAAGA 
GGAGGTTGTG CTAAAGGAAA AGGAGGATCG ATGCATATGT ATACCAAGAA CTTCTATGGG 
GGCAATGGCA TCGTCGGTGC ACAGGGCCCC CTGGGCGCTG GCATTGCTCT GGCCTGTAAA 
TATAAAGGAA ACGATGAGAT CTGTTTGACT TTATATGGGG ATGGCGCTGC GAATCAGGGG 
CAGATAGCCG AAGCTTTCAA TATGGCAGCT TTATGGAAAT TACCTTGTGT TTTCATCTGT 
GAGAATAACC TATATGGAAT GGGAACATCT ACTGAGAGAG CAGCAGCCAG CCCTGATTAC 
TACAAGAGGG GCAATTTTAT CCCTGGGCTA AAGGTCGATG GAATGGATGT TCTGTGTGTT 
CGTGAGGCAA CAAAATTTGC AGCTAACTAC TGTAGATCTG GAAAGGGGCC CATACTGATG 
GAGCTGCAAA CCTACCGTTA TCATGGACAC AGTATGAGTG ATCCTGGAGT CAGTTATCGT 
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGGGATC CTATAATAAT TCTCCAAGAT 
AGAATGGTAA ACAGCAAGCT CGCCACTGTG GAAGAATTAA AGGAAATTGG GGCTGAGGTG 
AGGAAAGAAA TTGATGATGC TGCCCAGTTT GCTACCACTG ATCCTGAGCC ACATTTGGAA 
GAATTAGGCC ATCACATCTA CAGCAGTGAT TCATCTTTTG AAGTTCGTGG TGCAAATCCA 
TGGATCAAGT TTAAGTCCGT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005381.1
CDS	64..1230
Translation	MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

Target ORF information:

RefSeq Version	NM_005390.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase E1 alpha 2 subunit (PDHA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005390.4

ORF Insert Sequence:

ATGCTGGCCG CCTTCATCTC CCGCGTGTTG AGGCGAGTTG CCCAGAAATC AGCTCGCAGA 
GTGCTGGTGG CATCCCGTAA CTCCTCAAAT GACGCTACAT TTGAAATTAA GAAATGTGAT 
CTTTATCTGT TGGAAGAGGG TCCCCCTGTC ACTACAGTGC TCACTAGGGC GGAGGGGCTT 
AAATACTACA GGATGATGCT GACTGTTCGC CGCATGGAAT TGAAGGCAGA TCAGCTGTAC 
AAACAGAAAT TCATTCGCGG TTTCTGTCAC CTGTGCGATG GTCAGGAAGC TTGTTGCGTG 
GGCCTTGAGG CCGGCATAAA CCCCTCGGAT CACGTCATTA CATCCTATAG GGCTCATGGT 
GTGTGCTATA CTCGGGGACT TTCTGTCCGA TCCATTCTCG CAGAGCTGAC GGGAAGAAGA 
GGAGGTTGTG CTAAAGGAAA AGGAGGATCG ATGCATATGT ATACCAAGAA CTTCTATGGG 
GGCAATGGCA TCGTCGGTGC ACAGGGCCCC CTGGGCGCTG GCATTGCTCT GGCCTGTAAA 
TATAAAGGAA ACGATGAGAT CTGTTTGACT TTATATGGGG ATGGCGCTGC GAATCAGGGG 
CAGATAGCCG AAGCTTTCAA TATGGCAGCT TTATGGAAAT TACCTTGTGT TTTCATCTGT 
GAGAATAACC TATATGGAAT GGGAACATCT ACTGAGAGAG CAGCAGCCAG CCCTGATTAC 
TACAAGAGGG GCAATTTTAT CCCTGGGCTA AAGGTCGATG GAATGGATGT TCTGTGTGTT 
CGTGAGGCAA CAAAATTTGC AGCTAACTAC TGTAGATCTG GAAAGGGGCC CATACTGATG 
GAGCTGCAAA CCTACCGTTA TCATGGACAC AGTATGAGTG ATCCTGGAGT CAGTTATCGT 
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGGGATC CTATAATAAT TCTCCAAGAT 
AGAATGGTAA ACAGCAAGCT CGCCACTGTG GAAGAATTAA AGGAAATTGG GGCTGAGGTG 
AGGAAAGAAA TTGATGATGC TGCCCAGTTT GCTACCACTG ATCCTGAGCC ACATTTGGAA 
GAATTAGGCC ATCACATCTA CAGCAGTGAT TCATCTTTTG AAGTTCGTGG TGCAAATCCA 
TGGATCAAGT TTAAGTCCGT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHA2 ( NM_005390.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005381.1 Homo sapiens pyruvate dehydrogenase E1 subunit alpha 2 (PDHA2), mRNA.

Price & Availability↑

CloneID	OHu30250
Clone ID Related Accession (Same CDS sequence)	NM_005390.4 , NM_005390.5
Accession Version	NM_005390.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC030697.2. On May 17, 2019 this sequence version replaced NM_005390.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC094760.1, SRR5189667.171651.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000295266.6/ ENSP00000295266.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGGCCG CCTTCATCTC CCGCGTGTTG AGGCGAGTTG CCCAGAAATC AGCTCGCAGA 
GTGCTGGTGG CATCCCGTAA CTCCTCAAAT GACGCTACAT TTGAAATTAA GAAATGTGAT 
CTTTATCTGT TGGAAGAGGG TCCCCCTGTC ACTACAGTGC TCACTAGGGC GGAGGGGCTT 
AAATACTACA GGATGATGCT GACTGTTCGC CGCATGGAAT TGAAGGCAGA TCAGCTGTAC 
AAACAGAAAT TCATTCGCGG TTTCTGTCAC CTGTGCGATG GTCAGGAAGC TTGTTGCGTG 
GGCCTTGAGG CCGGCATAAA CCCCTCGGAT CACGTCATTA CATCCTATAG GGCTCATGGT 
GTGTGCTATA CTCGGGGACT TTCTGTCCGA TCCATTCTCG CAGAGCTGAC GGGAAGAAGA 
GGAGGTTGTG CTAAAGGAAA AGGAGGATCG ATGCATATGT ATACCAAGAA CTTCTATGGG 
GGCAATGGCA TCGTCGGTGC ACAGGGCCCC CTGGGCGCTG GCATTGCTCT GGCCTGTAAA 
TATAAAGGAA ACGATGAGAT CTGTTTGACT TTATATGGGG ATGGCGCTGC GAATCAGGGG 
CAGATAGCCG AAGCTTTCAA TATGGCAGCT TTATGGAAAT TACCTTGTGT TTTCATCTGT 
GAGAATAACC TATATGGAAT GGGAACATCT ACTGAGAGAG CAGCAGCCAG CCCTGATTAC 
TACAAGAGGG GCAATTTTAT CCCTGGGCTA AAGGTCGATG GAATGGATGT TCTGTGTGTT 
CGTGAGGCAA CAAAATTTGC AGCTAACTAC TGTAGATCTG GAAAGGGGCC CATACTGATG 
GAGCTGCAAA CCTACCGTTA TCATGGACAC AGTATGAGTG ATCCTGGAGT CAGTTATCGT 
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGGGATC CTATAATAAT TCTCCAAGAT 
AGAATGGTAA ACAGCAAGCT CGCCACTGTG GAAGAATTAA AGGAAATTGG GGCTGAGGTG 
AGGAAAGAAA TTGATGATGC TGCCCAGTTT GCTACCACTG ATCCTGAGCC ACATTTGGAA 
GAATTAGGCC ATCACATCTA CAGCAGTGAT TCATCTTTTG AAGTTCGTGG TGCAAATCCA 
TGGATCAAGT TTAAGTCCGT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005381.1
CDS	59..1225
Translation	MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

Target ORF information:

RefSeq Version	NM_005390.5
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase E1 subunit alpha 2 (PDHA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005390.5

ORF Insert Sequence:

ATGCTGGCCG CCTTCATCTC CCGCGTGTTG AGGCGAGTTG CCCAGAAATC AGCTCGCAGA 
GTGCTGGTGG CATCCCGTAA CTCCTCAAAT GACGCTACAT TTGAAATTAA GAAATGTGAT 
CTTTATCTGT TGGAAGAGGG TCCCCCTGTC ACTACAGTGC TCACTAGGGC GGAGGGGCTT 
AAATACTACA GGATGATGCT GACTGTTCGC CGCATGGAAT TGAAGGCAGA TCAGCTGTAC 
AAACAGAAAT TCATTCGCGG TTTCTGTCAC CTGTGCGATG GTCAGGAAGC TTGTTGCGTG 
GGCCTTGAGG CCGGCATAAA CCCCTCGGAT CACGTCATTA CATCCTATAG GGCTCATGGT 
GTGTGCTATA CTCGGGGACT TTCTGTCCGA TCCATTCTCG CAGAGCTGAC GGGAAGAAGA 
GGAGGTTGTG CTAAAGGAAA AGGAGGATCG ATGCATATGT ATACCAAGAA CTTCTATGGG 
GGCAATGGCA TCGTCGGTGC ACAGGGCCCC CTGGGCGCTG GCATTGCTCT GGCCTGTAAA 
TATAAAGGAA ACGATGAGAT CTGTTTGACT TTATATGGGG ATGGCGCTGC GAATCAGGGG 
CAGATAGCCG AAGCTTTCAA TATGGCAGCT TTATGGAAAT TACCTTGTGT TTTCATCTGT 
GAGAATAACC TATATGGAAT GGGAACATCT ACTGAGAGAG CAGCAGCCAG CCCTGATTAC 
TACAAGAGGG GCAATTTTAT CCCTGGGCTA AAGGTCGATG GAATGGATGT TCTGTGTGTT 
CGTGAGGCAA CAAAATTTGC AGCTAACTAC TGTAGATCTG GAAAGGGGCC CATACTGATG 
GAGCTGCAAA CCTACCGTTA TCATGGACAC AGTATGAGTG ATCCTGGAGT CAGTTATCGT 
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGGGATC CTATAATAAT TCTCCAAGAT 
AGAATGGTAA ACAGCAAGCT CGCCACTGTG GAAGAATTAA AGGAAATTGG GGCTGAGGTG 
AGGAAAGAAA TTGATGATGC TGCCCAGTTT GCTACCACTG ATCCTGAGCC ACATTTGGAA 
GAATTAGGCC ATCACATCTA CAGCAGTGAT TCATCTTTTG AAGTTCGTGG TGCAAATCCA 
TGGATCAAGT TTAAGTCCGT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pyruvate dehydrogenase complex: mRNA and protein expression patterns of E1? subunit genes in human spermatogenesis.
Gene506(1)173-8(2012 Sep)
Pinheiro A,Silva MJ,Gra?a I,Silva J,S? R,Sousa M,Barros A,Tavares de Almeida I,Rivera I

Demethylation of the coding region triggers the activation of the human testis-specific PDHA2 gene in somatic tissues.
PloS one7(6)e38076(2012)
Pinheiro A,Nunes MJ,Milagre I,Rodrigues E,Silva MJ,de Almeida IT,Rivera I

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

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PDHA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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