PDHA1 cDNA ORF clone, Homo sapiens(human)

The following PDHA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10616 NM_001173456.1
Latest version!
Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$149.50-$209.30
$299.00
OHu11172 NM_001173454.1
Latest version!
Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu20621 NM_000284.4
Latest version!
Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu11667 NM_001173455.1
Latest version!
Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $237.30
$339.00
OHu99331 XM_017029574.2
Latest version!
Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu20621 NM_000284.3 Homo sapiens pyruvate dehydrogenase E1 alpha 1 subunit (PDHA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu99331 XM_017029574.1 Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10616
    Clone ID Related Accession (Same CDS sequence) NM_001173456.1
    Accession Version NM_001173456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK296457.1, AK296341.1, AL732326.4 and R49470.1. Transcript Variant: This variant (4) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (4) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.31856.1, SRR1803612.185639.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GGCCAGATAT TCGAAGCTTA CAACATGGCA
    GCTTTGTGGA AATTACCTTG TATTTTCATC TGTGAGAATA ATCGCTATGG AATGGGAACG
    TCTGTTGAGA GAGCGGCAGC CAGCACTGAT TACTACAAGA GAGGCGATTT CATTCCTGGG
    CTGAGAGTGG ATGGAATGGA TATCCTGTGC GTCCGAGAGG CAACAAGGTT TGCTGCTGCC
    TATTGTAGAT CTGGGAAGGG GCCCATCCTG ATGGAGCTGC AGACTTACCG TTACCACGGA
    CACAGTATGA GTGACCCTGG AGTCAGTTAC CGTACACGAG AAGAAATTCA GGAAGTAAGA
    AGTAAGAGTG ACCCTATTAT GCTTCTCAAG GACAGGATGG TGAACAGCAA TCTTGCCAGT
    GTGGAAGAAC TAAAGGAAAT TGATGTGGAA GTGAGGAAGG AGATTGAGGA TGCTGCCCAG
    TTTGCCACGG CCGATCCTGA GCCACCTTTG GAAGAGCTGG GCTACCACAT CTACTCCAGC
    GACCCACCTT TTGAAGTTCG TGGTGCCAAT CAGTGGATCA AGTTTAAGTC AGTCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001166927.1
    CDS146..1225
    Translation

    Target ORF information:

    RefSeq Version NM_001173456.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001173456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GGCCAGATAT TCGAAGCTTA CAACATGGCA
    GCTTTGTGGA AATTACCTTG TATTTTCATC TGTGAGAATA ATCGCTATGG AATGGGAACG
    TCTGTTGAGA GAGCGGCAGC CAGCACTGAT TACTACAAGA GAGGCGATTT CATTCCTGGG
    CTGAGAGTGG ATGGAATGGA TATCCTGTGC GTCCGAGAGG CAACAAGGTT TGCTGCTGCC
    TATTGTAGAT CTGGGAAGGG GCCCATCCTG ATGGAGCTGC AGACTTACCG TTACCACGGA
    CACAGTATGA GTGACCCTGG AGTCAGTTAC CGTACACGAG AAGAAATTCA GGAAGTAAGA
    AGTAAGAGTG ACCCTATTAT GCTTCTCAAG GACAGGATGG TGAACAGCAA TCTTGCCAGT
    GTGGAAGAAC TAAAGGAAAT TGATGTGGAA GTGAGGAAGG AGATTGAGGA TGCTGCCCAG
    TTTGCCACGG CCGATCCTGA GCCACCTTTG GAAGAGCTGG GCTACCACAT CTACTCCAGC
    GACCCACCTT TTGAAGTTCG TGGTGCCAAT CAGTGGATCA AGTTTAAGTC AGTCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11172
    Clone ID Related Accession (Same CDS sequence) NM_001173454.1
    Accession Version NM_001173454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK293250.1, BU170389.1, AL732326.4 and R49470.1. Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.53198.1, SRR1803613.115012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGTGCCC CTGGGCGCTG GGATTGCTCT AGCCTGTAAG
    TATAATGGAA AAGATGAGGT CTGCCTGACT TTATATGGCG ATGGTGCTGC TAACCAGGGC
    CAGATATTCG AAGCTTACAA CATGGCAGCT TTGTGGAAAT TACCTTGTAT TTTCATCTGT
    GAGAATAATC GCTATGGAAT GGGAACGTCT GTTGAGAGAG CGGCAGCCAG CACTGATTAC
    TACAAGAGAG GCGATTTCAT TCCTGGGCTG AGAGTGGATG GAATGGATAT CCTGTGCGTC
    CGAGAGGCAA CAAGGTTTGC TGCTGCCTAT TGTAGATCTG GGAAGGGGCC CATCCTGATG
    GAGCTGCAGA CTTACCGTTA CCACGGACAC AGTATGAGTG ACCCTGGAGT CAGTTACCGT
    ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGTGACC CTATTATGCT TCTCAAGGAC
    AGGATGGTGA ACAGCAATCT TGCCAGTGTG GAAGAACTAA AGGAAATTGA TGTGGAAGTG
    AGGAAGGAGA TTGAGGATGC TGCCCAGTTT GCCACGGCCG ATCCTGAGCC ACCTTTGGAA
    GAGCTGGGCT ACCACATCTA CTCCAGCGAC CCACCTTTTG AAGTTCGTGG TGCCAATCAG
    TGGATCAAGT TTAAGTCAGT CAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001166925.1
    CDS146..1432
    Translation

    Target ORF information:

    RefSeq Version NM_001173454.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001173454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGTGCCC CTGGGCGCTG GGATTGCTCT AGCCTGTAAG
    TATAATGGAA AAGATGAGGT CTGCCTGACT TTATATGGCG ATGGTGCTGC TAACCAGGGC
    CAGATATTCG AAGCTTACAA CATGGCAGCT TTGTGGAAAT TACCTTGTAT TTTCATCTGT
    GAGAATAATC GCTATGGAAT GGGAACGTCT GTTGAGAGAG CGGCAGCCAG CACTGATTAC
    TACAAGAGAG GCGATTTCAT TCCTGGGCTG AGAGTGGATG GAATGGATAT CCTGTGCGTC
    CGAGAGGCAA CAAGGTTTGC TGCTGCCTAT TGTAGATCTG GGAAGGGGCC CATCCTGATG
    GAGCTGCAGA CTTACCGTTA CCACGGACAC AGTATGAGTG ACCCTGGAGT CAGTTACCGT
    ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGTGACC CTATTATGCT TCTCAAGGAC
    AGGATGGTGA ACAGCAATCT TGCCAGTGTG GAAGAACTAA AGGAAATTGA TGTGGAAGTG
    AGGAAGGAGA TTGAGGATGC TGCCCAGTTT GCCACGGCCG ATCCTGAGCC ACCTTTGGAA
    GAGCTGGGCT ACCACATCTA CTCCAGCGAC CCACCTTTTG AAGTTCGTGG TGCCAATCAG
    TGGATCAAGT TTAAGTCAGT CAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20621
    Clone ID Related Accession (Same CDS sequence) NM_000284.4 , NM_000284.3
    Accession Version NM_000284.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, L13318.1, BU170389.1, AL732326.4 and R49470.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000284.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.267695.1, SRR1803615.9181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000422285.7/ ENSP00000394382.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
    TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
    CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
    ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
    GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
    TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
    CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
    TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
    AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
    GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
    TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
    AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000275.1
    CDS112..1284
    Translation

    Target ORF information:

    RefSeq Version NM_000284.4
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000284.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
    TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
    CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
    ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
    GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
    TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
    CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
    TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
    AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
    GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
    TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
    AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20621
    Clone ID Related Accession (Same CDS sequence) NM_000284.4 , NM_000284.3
    Accession Version NM_000284.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, L13318.1, BU170389.1, AL732326.4 and R49470.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced NM_000284.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.151307.1, SRR1803614.207600.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
    TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
    CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
    ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
    GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
    TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
    CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
    TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
    AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
    GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
    TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
    AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000275.1
    CDS146..1318
    Translation

    Target ORF information:

    RefSeq Version NM_000284.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 alpha 1 subunit (PDHA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000284.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
    TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
    CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
    CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
    TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
    TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
    CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
    ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
    GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
    TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
    CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
    TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
    AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
    GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
    TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
    AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11667
    Clone ID Related Accession (Same CDS sequence) NM_001173455.1
    Accession Version NM_001173455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK296341.1, AL732326.4 and R49470.1. Transcript Variant: This variant (3) uses an alternative acceptor splice site at one of the coding exons compared to variant 1, resulting in a longer isoform (3) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.216353.1, SRR1803616.95482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GTTTCTCCTT
    CCTCTAACAC AGGAAGCTTG CTGTGTGGGC CTGGAGGCCG GCATCAACCC CACAGACCAT
    CTCATCACAG CCTACCGGGC TCACGGCTTT ACTTTCACCC GGGGCCTTTC CGTCCGAGAA
    ATTCTCGCAG AGCTTACAGG ACGAAAAGGA GGTTGTGCTA AAGGGAAAGG AGGATCGATG
    CACATGTATG CCAAGAACTT CTACGGGGGC AATGGCATCG TGGGAGCGCA GGTGCCCCTG
    GGCGCTGGGA TTGCTCTAGC CTGTAAGTAT AATGGAAAAG ATGAGGTCTG CCTGACTTTA
    TATGGCGATG GTGCTGCTAA CCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001166926.1
    CDS146..1339
    Translation

    Target ORF information:

    RefSeq Version NM_001173455.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001173455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
    AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
    TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
    GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
    CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GTTTCTCCTT
    CCTCTAACAC AGGAAGCTTG CTGTGTGGGC CTGGAGGCCG GCATCAACCC CACAGACCAT
    CTCATCACAG CCTACCGGGC TCACGGCTTT ACTTTCACCC GGGGCCTTTC CGTCCGAGAA
    ATTCTCGCAG AGCTTACAGG ACGAAAAGGA GGTTGTGCTA AAGGGAAAGG AGGATCGATG
    CACATGTATG CCAAGAACTT CTACGGGGGC AATGGCATCG TGGGAGCGCA GGTGCCCCTG
    GGCGCTGGGA TTGCTCTAGC CTGTAAGTAT AATGGAAAAG ATGAGGTCTG CCTGACTTTA
    TATGGCGATG GTGCTGCTAA CCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99331
    Clone ID Related Accession (Same CDS sequence) XM_017029574.1 , XM_017029574.2
    Accession Version XM_017029574.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885063.1
    CDS133..1326
    Translation

    Target ORF information:

    RefSeq Version XM_017029574.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99331
    Clone ID Related Accession (Same CDS sequence) XM_017029574.1 , XM_017029574.2
    Accession Version XM_017029574.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885063.1
    CDS133..1326
    Translation

    Target ORF information:

    RefSeq Version XM_017029574.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029574.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
    CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
    CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
    GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
    CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
    AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
    AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
    GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
    TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
    GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
    GGCAATGGCA TCGTGGGAGC GCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
    TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
    GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
    GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
    AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
    ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
    AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
    GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
    ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
    CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.