PDE6D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6D
Entrez Gene ID	5147
Full Name	phosphodiesterase 6D
Synonyms	JBTS22,PDED
General protein information	Preferred Names phosphodiesterase 6D Names retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta GMP-PDE delta phosphodiesterase 6D, cGMP-specific, rod, delta protein p17
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014].
Disorder MIM:	602676
Disorder Html:	?Joubert syndrome 22, 615665 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002601.3	NP_002592.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1
NM_002601.4	NP_002592.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1
XM_011511342.1	XP_011509644.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform X1
NM_001291018.1	NP_001277947.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE6D	NP_002592.1
Canis lupus familiaris (dog)	PDE6D	NP_001003156.1
Mus musculus (house mouse)	Pde6d	NP_032827.1
Rattus norvegicus (Norway rat)	Pde6d	NP_001102276.1
Gallus gallus (chicken)	PDE6D	XP_422739.3
Drosophila melanogaster (fruit fly)	PrBP	NP_609246.1
Pan troglodytes (chimpanzee)	PDE6D	XP_001144711.1
Danio rerio (zebrafish)	pde6d	NP_001002708.1
	PDE6D	XP_001113642.1
Bos taurus (cattle)	PDE6D	NP_776845.1
Xenopus tropicalis (tropical clawed frog)	pde6d	XP_002937389.1
Anopheles gambiae (African malaria mosquito)	PDE6D_ANOGA	XP_320754.1
Caenorhabditis elegans (roundworm)	pdl-1	NP_495490.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Shuttling and sorting lipid-modified cargo into the cilia.
Stephen LA, Ismail S
Biochemical Society transactions44(5)1273-1280(2016 10)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
Thomas S, Wright KJ, Le Corre S, Micalizzi A, Romani M, Abhyankar A, Saada J, Perrault I, Amiel J, Litzler J, Filhol E, Elkhartoufi N, Kwong M, Casanova JL, Boddaert N, Baehr W, Lyonnet S, Munnich A, Burglen L, Chassaing N, Encha-Ravazi F, Vekemans M, Gleeson JG, Valente EM, Jackson PK, Drummond IA, Saunier S, Atti?-Bitach T
Human mutation35(1)137-46(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that the binding of UNC119 and PDE6D, to the lipid-modified ciliary cargo and the specific release of the cargo in the cilia by the ciliary small G-protein Arl3 in a GTP-dependent manner.
Title: Shuttling and sorting lipid-modified cargo into the cilia.

Study identifies PDE6D as a novel Joubert syndrome gene and provides the first evidence of prenyl-binding-dependent trafficking in ciliopathies.
Title: A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.

RPGR is acting as a scaffold protein recruiting cargo-loaded PDE6D and Arl3 to release lipidated cargo into cilia.
Title: The interplay between RPGR, PDE? and Arl2/3 regulate the ciliary targeting of farnesylated cargo.

findings indicate that ARL13B, INPP5E, PDE6D, and CEP164 form a distinct functional network that is involved in JBTS and NPHP but independent of the ones previously defined by NPHP and MKS proteins
Title: ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Data show that PDEdelta activity augments K/Hras signalling by enriching Ras at the plasma membrane.
Title: The GDI-like solubilizing factor PDE? sustains the spatial organization and signalling of Ras family proteins.

The following PDE6D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24109	NM_002601.4 Latest version!	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu59578	XM_011511342.1 Latest version!	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu23708	NM_001291018.1 Latest version!	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu24109	NM_002601.3	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6D ( NM_002601.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002592.1 Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24109
Clone ID Related Accession (Same CDS sequence)	NM_002601.3 , NM_002601.4
Accession Version	NM_002601.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037751.1 and BC007831.2. This sequence is a reference standard in the RefSeqGene project. On Mar 22, 2014 this sequence version replaced NM_002601.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007831.2, SRR1163658.270467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGAATGG TTCTTCGAGT TTGGCTTTGT GATCCCTAAC 
TCCACAAATA CCTGGCAGTC CTTGATAGAG GCAGCACCCG AGTCCCAGAT GATGCCAGCA 
AGCGTCTTAA CTGGGAACGT TATCATAGAA ACAAAGTTTT TTGACGACGA TCTTCTTGTA 
AGCACATCCA GAGTGAGACT TTTCTATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002592.1
CDS	214..666
Translation	MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Target ORF information:

RefSeq Version	NM_002601.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002601.3

ORF Insert Sequence:

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGAATGG TTCTTCGAGT TTGGCTTTGT GATCCCTAAC 
TCCACAAATA CCTGGCAGTC CTTGATAGAG GCAGCACCCG AGTCCCAGAT GATGCCAGCA 
AGCGTCTTAA CTGGGAACGT TATCATAGAA ACAAAGTTTT TTGACGACGA TCTTCTTGTA 
AGCACATCCA GAGTGAGACT TTTCTATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6D ( NM_002601.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002592.1 Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24109
Clone ID Related Accession (Same CDS sequence)	NM_002601.3 , NM_002601.4
Accession Version	NM_002601.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037751.1 and BC007831.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002601.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.164007.1, SRR1163655.302877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000287600.9/ ENSP00000287600.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGAATGG TTCTTCGAGT TTGGCTTTGT GATCCCTAAC 
TCCACAAATA CCTGGCAGTC CTTGATAGAG GCAGCACCCG AGTCCCAGAT GATGCCAGCA 
AGCGTCTTAA CTGGGAACGT TATCATAGAA ACAAAGTTTT TTGACGACGA TCTTCTTGTA 
AGCACATCCA GAGTGAGACT TTTCTATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002592.1
CDS	169..621
Translation	MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Target ORF information:

RefSeq Version	NM_002601.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002601.4

ORF Insert Sequence:

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGAATGG TTCTTCGAGT TTGGCTTTGT GATCCCTAAC 
TCCACAAATA CCTGGCAGTC CTTGATAGAG GCAGCACCCG AGTCCCAGAT GATGCCAGCA 
AGCGTCTTAA CTGGGAACGT TATCATAGAA ACAAAGTTTT TTGACGACGA TCTTCTTGTA 
AGCACATCCA GAGTGAGACT TTTCTATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6D ( XM_011511342.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509644.1 Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59578
Clone ID Related Accession (Same CDS sequence)	XM_011511342.1
Accession Version	XM_011511342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTTC GGGATGCTGA GACAGGGAAG ATACTCTGGC AAGGAACAGA AGACCTGTCT 
GTCCCTGGTG TGGAGCATGA AGCCCGTGTT CCCAAGAAAA TCCTCAAGTG CAAGGCAGTG 
TCTCGAGAAC TTAATTTTTC TTCGACAGAA CAAATGGAAA AATTCCGCCT GGAACAAAAA 
GTTTACTTCA AAGGGCAATG CCTAGAAGAA TGGTTCTTCG AGTTTGGCTT TGTGATCCCT 
AACTCCACAA ATACCTGGCA GTCCTTGATA GAGGCAGCAC CCGAGTCCCA GATGATGCCA 
GCAAGCGTCT TAACTGGGAA CGTTATCATA GAAACAAAGT TTTTTGACGA CGATCTTCTT 
GTAAGCACAT CCAGAGTGAG ACTTTTCTAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509644.1
CDS	752..1147
Translation	MNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Target ORF information:

RefSeq Version	XM_011511342.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511342.1

ORF Insert Sequence:

ATGAACCTTC GGGATGCTGA GACAGGGAAG ATACTCTGGC AAGGAACAGA AGACCTGTCT 
GTCCCTGGTG TGGAGCATGA AGCCCGTGTT CCCAAGAAAA TCCTCAAGTG CAAGGCAGTG 
TCTCGAGAAC TTAATTTTTC TTCGACAGAA CAAATGGAAA AATTCCGCCT GGAACAAAAA 
GTTTACTTCA AAGGGCAATG CCTAGAAGAA TGGTTCTTCG AGTTTGGCTT TGTGATCCCT 
AACTCCACAA ATACCTGGCA GTCCTTGATA GAGGCAGCAC CCGAGTCCCA GATGATGCCA 
GCAAGCGTCT TAACTGGGAA CGTTATCATA GAAACAAAGT TTTTTGACGA CGATCTTCTT 
GTAAGCACAT CCAGAGTGAG ACTTTTCTAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6D ( NM_001291018.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277947.1 Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23708
Clone ID Related Accession (Same CDS sequence)	NM_001291018.1
Accession Version	NM_001291018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037751.1, BI754373.1, AC073476.6 and BC007831.2. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.19778.1, SRR1163658.174528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2149398 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGTGGGA ACGTTATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277947.1
CDS	214..495
Translation	MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEVGTLS

Target ORF information:

RefSeq Version	NM_001291018.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 6D (PDE6D), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291018.1

ORF Insert Sequence:

ATGTCAGCCA AGGACGAGCG GGCCAGGGAG ATCCTGAGGG GCTTCAAACT AAATTGGATG 
AACCTTCGGG ATGCTGAGAC AGGGAAGATA CTCTGGCAAG GAACAGAAGA CCTGTCTGTC 
CCTGGTGTGG AGCATGAAGC CCGTGTTCCC AAGAAAATCC TCAAGTGCAA GGCAGTGTCT 
CGAGAACTTA ATTTTTCTTC GACAGAACAA ATGGAAAAAT TCCGCCTGGA ACAAAAAGTT 
TACTTCAAAG GGCAATGCCT AGAAGTGGGA ACGTTATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Shuttling and sorting lipid-modified cargo into the cilia.
Biochemical Society transactions44(5)1273-1280(2016 10)
Stephen LA,Ismail S

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
Human mutation35(1)137-46(2014 Jan)
Thomas S,Wright KJ,Le Corre S,Micalizzi A,Romani M,Abhyankar A,Saada J,Perrault I,Amiel J,Litzler J,Filhol E,Elkhartoufi N,Kwong M,Casanova JL,Boddaert N,Baehr W,Lyonnet S,Munnich A,Burglen L,Chassaing N,Encha-Ravazi F,Vekemans M,Gleeson JG,Valente EM,Jackson PK,Drummond IA,Saunier S,Atti?-Bitach T

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PDE6D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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