LCMT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LCMT1
Entrez Gene ID	51451
Full Name	leucine carboxyl methyltransferase 1
Synonyms	CGI-68,LCMT,PPMT1
General protein information	Preferred Names leucine carboxyl methyltransferase 1 Names leucine carboxyl methyltransferase 1 [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1 protein phosphatase methyltransferase 1 protein-leucine O-methyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.1
Summary	LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610286

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032391.1	NP_001027563.1	leucine carboxyl methyltransferase 1 isoform b
XM_011545864.1	XP_011544166.1	leucine carboxyl methyltransferase 1 isoform X1
NM_001032391.2	NP_001027563.1	leucine carboxyl methyltransferase 1 isoform b
XM_005255354.3	XP_005255411.1	leucine carboxyl methyltransferase 1 isoform X3
XM_005255354.4	XP_005255411.1	leucine carboxyl methyltransferase 1 isoform X3
XM_011545862.2	XP_011544164.1	leucine carboxyl methyltransferase 1 isoform X2
NM_016309.3	NP_057393.2	leucine carboxyl methyltransferase 1 isoform a
NM_016309.2	NP_057393.2	leucine carboxyl methyltransferase 1 isoform a

Pathways from BioSystems

Homologies

	LCMT1	XP_001099127.1
Drosophila melanogaster (fruit fly)	CG3793	NP_609766.1
Caenorhabditis elegans (roundworm)	CELE_B0285.4	NP_497875.1
Oryza sativa (rice)	Os06g0140100	NP_001056749.1
Xenopus tropicalis (tropical clawed frog)	lcmt1	NP_001016033.1
Pan troglodytes (chimpanzee)	LCMT1	XP_001164891.1
Canis lupus familiaris (dog)	LCMT1	XP_005621453.1
Bos taurus (cattle)	LCMT1	NP_001069956.1
Mus musculus (house mouse)	Lcmt1	NP_079580.2
Gallus gallus (chicken)	LCMT1	NP_001006167.2
Arabidopsis thaliana (thale cress)	AT1G02100	NP_973741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008768	XP_314892.4
Homo sapiens (human)	LCMT1	NP_057393.2
Rattus norvegicus (Norway rat)	Lcmt1	NP_955437.1
Danio rerio (zebrafish)	lcmt1	NP_001004645.1

Gene Ontology
Bibliography

Related articles in PubMed

NNMT Silencing Activates Tumor Suppressor PP2A, Inactivates Oncogenic STKs, and Inhibits Tumor Forming Ability.
Palanichamy K, Kanji S, Gordon N, Thirumoorthy K, Jacob JR, Litzenberg KT, Patel D, Chakravarti A
Clinical cancer research : an official journal of the American Association for Cancer Research23(9)2325-2334(2017 May)

A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size.
Xia X, Gholkar A, Senese S, Torres JZ
Cell cycle (Georgetown, Tex.)14(12)1938-47(2015)

Leucine carboxyl methyltransferase 1 (LCMT1)-dependent methylation regulates the association of protein phosphatase 2A and Tau protein with plasma membrane microdomains in neuroblastoma cells.
Sontag JM, Nunbhakdi-Craig V, Sontag E
The Journal of biological chemistry288(38)27396-405(2013 Sep)

The biogenesis of active protein phosphatase 2A holoenzymes: a tightly regulated process creating phosphatase specificity.
Sents W, Ivanova E, Lambrecht C, Haesen D, Janssens V
The FEBS journal280(2)644-61(2013 Jan)

Glycogen synthase kinase-3? regulates leucine-309 demethylation of protein phosphatase-2A via PPMT1 and PME-1.
Yao XQ, Li XC, Zhang XX, Yin YY, Liu B, Luo DJ, Wang Q, Wang JZ, Liu GP
FEBS letters586(16)2522-8(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division
Title: A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size.

alterations in the membrane localization of PP2A and Tau following down-regulation of LCMT1 may lead to PP2A and Tau dysfunction in AD.
Title: Leucine carboxyl methyltransferase 1 (LCMT1)-dependent methylation regulates the association of protein phosphatase 2A and Tau protein with plasma membrane microdomains in neuroblastoma cells.

Data indicate that PP2A holoenzyme biogenesis and activity are controlled by five PP2A modulators, consisting of alpha4, PTPA, LCMT1, PME-1 and TIPRL1, which serve to prevent promiscuous phosphatase activity until the holoenzyme is completely assembled.
Title: The biogenesis of active protein phosphatase 2A holoenzymes: a tightly regulated process creating phosphatase specificity.

GSK-3beta can inhibit PP2A by increasing the inhibitory L309-demethylation involving upregulation of PME-1 and inhibition of PPMT1
Title: Glycogen synthase kinase-3? regulates leucine-309 demethylation of protein phosphatase-2A via PPMT1 and PME-1.

determined crystal structures of human LCMT-1 in isolation and in complex with PP2A stabilized by a cofactor mimic. The structures show that the LCMT-1 active-site pocket recognizes the carboxyl terminus of PP2A
Title: The structural basis for tight control of PP2A methylation and function by LCMT-1.

The following LCMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LCMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30821	NM_016309.3 Latest version!	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu49145	XM_005255354.4 Latest version!	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu30839	NM_001032391.2 Latest version!	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu67945	XM_011545862.2 Latest version!	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu67947	XM_011545864.1 Latest version!	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu30839	NM_001032391.1	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu49145	XM_005255354.3	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu30821	NM_016309.2	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LCMT1 ( NM_016309.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057393.2 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30821
Clone ID Related Accession (Same CDS sequence)	NM_016309.3 , NM_016309.2
Accession Version	NM_016309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI459868.1 and BC001214.1. On Nov 23, 2018 this sequence version replaced NM_016309.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR5189652.215033.1, SRR3476690.168244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399069.8/ ENSP00000382021.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057393.2
CDS	143..1147
Translation	MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	NM_016309.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016309.3

ORF Insert Sequence:

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( NM_016309.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057393.2 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30821
Clone ID Related Accession (Same CDS sequence)	NM_016309.3 , NM_016309.2
Accession Version	NM_016309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI459868.1, BC001214.1 and BF058787.1. On Sep 1, 2005 this sequence version replaced NM_016309.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK225271.1, AF151826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057393.2
CDS	159..1163
Translation	MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	NM_016309.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016309.2

ORF Insert Sequence:

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( XM_005255354.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255411.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu49145
Clone ID Related Accession (Same CDS sequence)	XM_005255354.3 , XM_005255354.4
Accession Version	XM_005255354.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255411.1
CDS	248..952
Translation	MDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	XM_005255354.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255354.3

ORF Insert Sequence:

ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( XM_005255354.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255411.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu49145
Clone ID Related Accession (Same CDS sequence)	XM_005255354.3 , XM_005255354.4
Accession Version	XM_005255354.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005255354.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255411.1
CDS	255..959
Translation	MDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	XM_005255354.4
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255354.4

ORF Insert Sequence:

ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATGCAA GCCTCCCCTA 
TCCAGCCCCA TTCTAGAACT GCATTCAGAG GACACACTTC AGATGGATGG ACACATACTG 
GATTCAAAGA GATATGCCGT TATTGGAGCA GATCTCCGAG ACCTGTCTGA ACTGGAAGAG 
AAGCTAAAGA AATGTAACAT GAATACACAA TTGCCAACAC TCCTGATAGC TGAATGTGTG 
CTGGTTTACA TGACTCCAGA GCAGTCCGCA AACCTCCTGA AGTGGGCAGC CAACAGTTTT 
GAGAGAGCCA TGTTCATAAA CTACGAACAG GTGAACATGG GTGATCGGTT TGGGCAGATC 
ATGATTGAAA ACCTGCGGAG ACGCCAGTGT GACCTGGCGG GAGTGGAGAC CTGCAAGTCA 
TTAGAGTCAC AGAAAGAACG GCTCCTGTCG AATGGGTGGG AAACAGCATC GGCCGTCGAC 
ATGATGGAGT TGTACAACAG GTTACCTCGA GCTGAAGTGA GCAGGATAGA ATCACTTGAA 
TTCCTGGATG AAATGGAGCT GCTGGAGCAG CTCATGCGGC ATTACTGCCT TTGCTGGGCA 
ACCAAAGGAG GAAATGAGCT TGGGCTGAAG GAGATAACTT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( NM_001032391.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001027563.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30839
Clone ID Related Accession (Same CDS sequence)	NM_001032391.1 , NM_001032391.2
Accession Version	NM_001032391.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI459868.1, AL560574.3, BC001214.1 and BF058787.1. Transcript Variant: This variant (2) lacks two alternate internal exons that encode an in-frame segment, compared to variant 1. The resulting isoform (b) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: ERR279876.6228.1, AL582093.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATTGCC AACACTCCTG 
ATAGCTGAAT GTGTGCTGGT TTACATGACT CCAGAGCAGT CCGCAAACCT CCTGAAGTGG 
GCAGCCAACA GTTTTGAGAG AGCCATGTTC ATAAACTACG AACAGGTGAA CATGGGTGAT 
CGGTTTGGGC AGATCATGAT TGAAAACCTG CGGAGACGCC AGTGTGACCT GGCGGGAGTG 
GAGACCTGCA AGTCATTAGA GTCACAGAAA GAACGGCTCC TGTCGAATGG GTGGGAAACA 
GCATCGGCCG TCGACATGAT GGAGTTGTAC AACAGGTTAC CTCGAGCTGA AGTGAGCAGG 
ATAGAATCAC TTGAATTCCT GGATGAAATG GAGCTGCTGG AGCAGCTCAT GCGGCATTAC 
TGCCTTTGCT GGGCAACCAA AGGAGGAAAT GAGCTTGGGC TGAAGGAGAT AACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027563.1
CDS	159..998
Translation	MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	NM_001032391.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032391.1

ORF Insert Sequence:

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATTGCC AACACTCCTG 
ATAGCTGAAT GTGTGCTGGT TTACATGACT CCAGAGCAGT CCGCAAACCT CCTGAAGTGG 
GCAGCCAACA GTTTTGAGAG AGCCATGTTC ATAAACTACG AACAGGTGAA CATGGGTGAT 
CGGTTTGGGC AGATCATGAT TGAAAACCTG CGGAGACGCC AGTGTGACCT GGCGGGAGTG 
GAGACCTGCA AGTCATTAGA GTCACAGAAA GAACGGCTCC TGTCGAATGG GTGGGAAACA 
GCATCGGCCG TCGACATGAT GGAGTTGTAC AACAGGTTAC CTCGAGCTGA AGTGAGCAGG 
ATAGAATCAC TTGAATTCCT GGATGAAATG GAGCTGCTGG AGCAGCTCAT GCGGCATTAC 
TGCCTTTGCT GGGCAACCAA AGGAGGAAAT GAGCTTGGGC TGAAGGAGAT AACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( NM_001032391.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001027563.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30839
Clone ID Related Accession (Same CDS sequence)	NM_001032391.1 , NM_001032391.2
Accession Version	NM_001032391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI459868.1, AL560574.3 and BC001214.1. On May 31, 2019 this sequence version replaced NM_001032391.1. Transcript Variant: This variant (2) lacks two alternate internal exons that encode an in-frame segment, compared to variant 1. The resulting isoform (b) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.585301.1, ERR279876.6228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATTGCC AACACTCCTG 
ATAGCTGAAT GTGTGCTGGT TTACATGACT CCAGAGCAGT CCGCAAACCT CCTGAAGTGG 
GCAGCCAACA GTTTTGAGAG AGCCATGTTC ATAAACTACG AACAGGTGAA CATGGGTGAT 
CGGTTTGGGC AGATCATGAT TGAAAACCTG CGGAGACGCC AGTGTGACCT GGCGGGAGTG 
GAGACCTGCA AGTCATTAGA GTCACAGAAA GAACGGCTCC TGTCGAATGG GTGGGAAACA 
GCATCGGCCG TCGACATGAT GGAGTTGTAC AACAGGTTAC CTCGAGCTGA AGTGAGCAGG 
ATAGAATCAC TTGAATTCCT GGATGAAATG GAGCTGCTGG AGCAGCTCAT GCGGCATTAC 
TGCCTTTGCT GGGCAACCAA AGGAGGAAAT GAGCTTGGGC TGAAGGAGAT AACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027563.1
CDS	143..982
Translation	MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	NM_001032391.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032391.2

ORF Insert Sequence:

ATGGCCACTA GGCAGAGGGA ATCCTCTATC ACCTCCTGCT GTTCCACCTC GAGCTGCGAC 
GCAGACGACG AGGGCGTGCG CGGCACCTGC GAAGATGCTT CCCTGTGCAA GAGGTTTGCA 
GTAAGCATTG GCTACTGGCA TGACCCTTAC ATACAGCACT TTGTGAGACT GTCTAAAGAG 
AGGAAAGCCC CTGAAATCAA CAGAGGATAT TTTGCTCGAG TCCATGGTGT CAGTCAGCTT 
ATAAAGGCAT TTCTACGGAA GACAGAATGT CATTGTCAAA TTGTCAACCT TGGGGCAGGC 
ATGGATACCA CCTTCTGGAG ATTAAAGGAT GAAGATCTTC TCCCAAGTAA ATATTTTGAG 
GTTGACTTTC CAATGATTGT CACGAGAAAG CTGCACAGTA TCAAATTGCC AACACTCCTG 
ATAGCTGAAT GTGTGCTGGT TTACATGACT CCAGAGCAGT CCGCAAACCT CCTGAAGTGG 
GCAGCCAACA GTTTTGAGAG AGCCATGTTC ATAAACTACG AACAGGTGAA CATGGGTGAT 
CGGTTTGGGC AGATCATGAT TGAAAACCTG CGGAGACGCC AGTGTGACCT GGCGGGAGTG 
GAGACCTGCA AGTCATTAGA GTCACAGAAA GAACGGCTCC TGTCGAATGG GTGGGAAACA 
GCATCGGCCG TCGACATGAT GGAGTTGTAC AACAGGTTAC CTCGAGCTGA AGTGAGCAGG 
ATAGAATCAC TTGAATTCCT GGATGAAATG GAGCTGCTGG AGCAGCTCAT GCGGCATTAC 
TGCCTTTGCT GGGCAACCAA AGGAGGAAAT GAGCTTGGGC TGAAGGAGAT AACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( XM_011545862.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544164.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67945
Clone ID Related Accession (Same CDS sequence)	XM_011545862.2
Accession Version	XM_011545862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCCT GTGCAAGAGA ACTGCCATCA GCAGCGCCCG ACAGCACTCT CCTCCTCACT 
GCCCAACACT GGGTGGCAAA TCTTTTCATC TTGGCCACTC TGATAGGCTG GACAGTGAGG 
TCCTTCTTCC TAGGGCGCAT GGAAACCTGT TGCAGTCACC TGGGTTTGCG GAGTGGGAGG 
AGGTTTGCAG TAAGCATTGG CTACTGGCAT GACCCTTACA TACAGCACTT TGTGAGACTG 
TCTAAAGAGA GGAAAGCCCC TGAAATCAAC AGAGGATATT TTGCTCGAGT CCATGGTGTC 
AGTCAGCTTA TAAAGGCATT TCTACGGAAG ACAGAATGTC ATTGTCAAAT TGTCAACCTT 
GGGGCAGGCA TGGATACCAC CTTCTGGAGA TTAAAGGATG AAGATCTTCT CCCAAGTAAA 
TATTTTGAGG TTGACTTTCC AATGATTGTC ACGAGAAAGC TGCACAGTAT CAAATTGCCA 
ACACTCCTGA TAGCTGAATG TGTGCTGGTT TACATGACTC CAGAGCAGTC CGCAAACCTC 
CTGAAGTGGG CAGCCAACAG TTTTGAGAGA GCCATGTTCA TAAACTACGA ACAGGTGAAC 
ATGGGTGATC GGTTTGGGCA GATCATGATT GAAAACCTGC GGAGACGCCA GTGTGACCTG 
GCGGGAGTGG AGACCTGCAA GTCATTAGAG TCACAGAAAG AACGGCTCCT GTCGAATGGG 
TGGGAAACAG CATCGGCCGT CGACATGATG GAGTTGTACA ACAGGTTACC TCGAGCTGAA 
GTGAGCAGGA TAGAATCACT TGAATTCCTG GATGAAATGG AGCTGCTGGA GCAGCTCATG 
CGGCATTACT GCCTTTGCTG GGCAACCAAA GGAGGAAATG AGCTTGGGCT GAAGGAGATA 
ACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544164.1
CDS	204..1112
Translation	MLPCARELPSAAPDSTLLLTAQHWVANLFILATLIGWTVRSFFLGRMETCCSHLGLRSGRRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	XM_011545862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545862.2

ORF Insert Sequence:

ATGCTTCCCT GTGCAAGAGA ACTGCCATCA GCAGCGCCCG ACAGCACTCT CCTCCTCACT 
GCCCAACACT GGGTGGCAAA TCTTTTCATC TTGGCCACTC TGATAGGCTG GACAGTGAGG 
TCCTTCTTCC TAGGGCGCAT GGAAACCTGT TGCAGTCACC TGGGTTTGCG GAGTGGGAGG 
AGGTTTGCAG TAAGCATTGG CTACTGGCAT GACCCTTACA TACAGCACTT TGTGAGACTG 
TCTAAAGAGA GGAAAGCCCC TGAAATCAAC AGAGGATATT TTGCTCGAGT CCATGGTGTC 
AGTCAGCTTA TAAAGGCATT TCTACGGAAG ACAGAATGTC ATTGTCAAAT TGTCAACCTT 
GGGGCAGGCA TGGATACCAC CTTCTGGAGA TTAAAGGATG AAGATCTTCT CCCAAGTAAA 
TATTTTGAGG TTGACTTTCC AATGATTGTC ACGAGAAAGC TGCACAGTAT CAAATTGCCA 
ACACTCCTGA TAGCTGAATG TGTGCTGGTT TACATGACTC CAGAGCAGTC CGCAAACCTC 
CTGAAGTGGG CAGCCAACAG TTTTGAGAGA GCCATGTTCA TAAACTACGA ACAGGTGAAC 
ATGGGTGATC GGTTTGGGCA GATCATGATT GAAAACCTGC GGAGACGCCA GTGTGACCTG 
GCGGGAGTGG AGACCTGCAA GTCATTAGAG TCACAGAAAG AACGGCTCCT GTCGAATGGG 
TGGGAAACAG CATCGGCCGT CGACATGATG GAGTTGTACA ACAGGTTACC TCGAGCTGAA 
GTGAGCAGGA TAGAATCACT TGAATTCCTG GATGAAATGG AGCTGCTGGA GCAGCTCATG 
CGGCATTACT GCCTTTGCTG GGCAACCAAA GGAGGAAATG AGCTTGGGCT GAAGGAGATA 
ACTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCMT1 ( XM_011545864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544166.1 Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67947
Clone ID Related Accession (Same CDS sequence)	XM_011545864.1
Accession Version	XM_011545864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	leucine carboxyl methyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCCT GTGCAAGAGA ACTGCCATCA GCAGCGCCCG ACAGCACTCT CCTCCTCACT 
GCCCAACACT GGGTGGCAAA TCTTTTCATC TTGGCCACTC TGATAGGCTG GACAGTGAGG 
TCCTTCTTCC TAGGGCGCAT GGAAACCTGT TGCAGTCACC TGGGTTTGCG GAGTGGGAGG 
AGGTTTGCAG TAAGCATTGG CTACTGGCAT GACCCTTACA TACAGCACTT TGTGAGACTG 
TCTAAAGAGA GGAAAGCCCC TGAAATCAAC AGAGGATATT TTGCTCGAGT CCATGGTGTC 
AGTCAGCTTA TAAAGGCATT TCTACGGAAG ACAGAATGTC ATTGTCAAAT TGTCAACCTT 
GGGGCAGGCA TGGATACCAC CTTCTGGAGA TTAAAGGATG AAGATCTTCT CCCAAGTAAA 
TATTTTGAGG TTGACTTTCC AATGATTGTC ACGAGAAAGC TGCACAGTAT CAAATGCAAG 
CCTCCCCTAT CCAGCCCCAT TCTAGAACTG CATTCAGAGG ACACACTTCA GATGGATGGA 
CACATACTGG ATTCAAAGAG ATATGCCGTT ATTGGAGCAG ATCTCCGAGA CCTGTCTGAA 
CTGGAAGAGA AGCTAAAGAA ATGTAACATG AATACACAAT TGCCAACACT CCTGATAGCT 
GAATGTGTGC TGGTTTACAT GACTCCAGAG CAGTCCGCAA ACCTCCTGAA GTGGGCAGCC 
AACAGTTTTG AGAGAGCCAT GTTCATAAAC TACGAACAGG TGAACATGGG TGATCGGTTT 
GGGCAGATCA TGATTGAAAA CCTGCGGAGA CGCCAGTGTG ACCTGGCGGG AGTGGAGACC 
TGCAAGTCAT TAGAGTCACA GAAAGAACGG CTCCTGTCGA ATGGGTGGGA AACAGCATCG 
GCCGTCGACA TGATGGAGTT GTACAACAGG TTACCTCGAG CTGAAGTGAG CAGGATAGAA 
TCACTTGAAT TCCTGGATGA AATGGAGCTG CTGGAGCAGC TCATGCGGCA TTACTGCCTT 
TGCTGGGCAA CCAAAGGAGG AAATGAGCTT GGGCTGAAGG AGATAACTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544166.1
CDS	204..1277
Translation	MLPCARELPSAAPDSTLLLTAQHWVANLFILATLIGWTVRSFFLGRMETCCSHLGLRSGRRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY

Target ORF information:

RefSeq Version	XM_011545864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545864.1

ORF Insert Sequence:

ATGCTTCCCT GTGCAAGAGA ACTGCCATCA GCAGCGCCCG ACAGCACTCT CCTCCTCACT 
GCCCAACACT GGGTGGCAAA TCTTTTCATC TTGGCCACTC TGATAGGCTG GACAGTGAGG 
TCCTTCTTCC TAGGGCGCAT GGAAACCTGT TGCAGTCACC TGGGTTTGCG GAGTGGGAGG 
AGGTTTGCAG TAAGCATTGG CTACTGGCAT GACCCTTACA TACAGCACTT TGTGAGACTG 
TCTAAAGAGA GGAAAGCCCC TGAAATCAAC AGAGGATATT TTGCTCGAGT CCATGGTGTC 
AGTCAGCTTA TAAAGGCATT TCTACGGAAG ACAGAATGTC ATTGTCAAAT TGTCAACCTT 
GGGGCAGGCA TGGATACCAC CTTCTGGAGA TTAAAGGATG AAGATCTTCT CCCAAGTAAA 
TATTTTGAGG TTGACTTTCC AATGATTGTC ACGAGAAAGC TGCACAGTAT CAAATGCAAG 
CCTCCCCTAT CCAGCCCCAT TCTAGAACTG CATTCAGAGG ACACACTTCA GATGGATGGA 
CACATACTGG ATTCAAAGAG ATATGCCGTT ATTGGAGCAG ATCTCCGAGA CCTGTCTGAA 
CTGGAAGAGA AGCTAAAGAA ATGTAACATG AATACACAAT TGCCAACACT CCTGATAGCT 
GAATGTGTGC TGGTTTACAT GACTCCAGAG CAGTCCGCAA ACCTCCTGAA GTGGGCAGCC 
AACAGTTTTG AGAGAGCCAT GTTCATAAAC TACGAACAGG TGAACATGGG TGATCGGTTT 
GGGCAGATCA TGATTGAAAA CCTGCGGAGA CGCCAGTGTG ACCTGGCGGG AGTGGAGACC 
TGCAAGTCAT TAGAGTCACA GAAAGAACGG CTCCTGTCGA ATGGGTGGGA AACAGCATCG 
GCCGTCGACA TGATGGAGTT GTACAACAGG TTACCTCGAG CTGAAGTGAG CAGGATAGAA 
TCACTTGAAT TCCTGGATGA AATGGAGCTG CTGGAGCAGC TCATGCGGCA TTACTGCCTT 
TGCTGGGCAA CCAAAGGAGG AAATGAGCTT GGGCTGAAGG AGATAACTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NNMT Silencing Activates Tumor Suppressor PP2A, Inactivates Oncogenic STKs, and Inhibits Tumor Forming Ability.
Clinical cancer research : an official journal of the American Association for Cancer Research23(9)2325-2334(2017 May)
Palanichamy K,Kanji S,Gordon N,Thirumoorthy K,Jacob JR,Litzenberg KT,Patel D,Chakravarti A

A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size.
Cell cycle (Georgetown, Tex.)14(12)1938-47(2015)
Xia X,Gholkar A,Senese S,Torres JZ

Leucine carboxyl methyltransferase 1 (LCMT1)-dependent methylation regulates the association of protein phosphatase 2A and Tau protein with plasma membrane microdomains in neuroblastoma cells.
The Journal of biological chemistry288(38)27396-405(2013 Sep)
Sontag JM,Nunbhakdi-Craig V,Sontag E

The biogenesis of active protein phosphatase 2A holoenzymes: a tightly regulated process creating phosphatase specificity.
The FEBS journal280(2)644-61(2013 Jan)
Sents W,Ivanova E,Lambrecht C,Haesen D,Janssens V

Glycogen synthase kinase-3? regulates leucine-309 demethylation of protein phosphatase-2A via PPMT1 and PME-1.
FEBS letters586(16)2522-8(2012 Jul)
Yao XQ,Li XC,Zhang XX,Yin YY,Liu B,Luo DJ,Wang Q,Wang JZ,Liu GP

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LCMT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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