PDE3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE3A
Entrez Gene ID	5139
Full Name	phosphodiesterase 3A
Synonyms	CGI-PDE,CGI-PDE A,CGI-PDE-A,HTNB
General protein information	Preferred Names phosphodiesterase 3A Names cGMP-inhibited 3',5'-cyclic phosphodiesterase A cAMP phosphodiesterase, myocardial cGMP-inhibited cyclic GMP-inhibited phosphodiesterase A phosphodiesterase 3A, cGMP-inhibited
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p12.2
Summary	This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011].
Disorder MIM:	123805
Disorder Html:	Hypertension and brachydactyly syndrome, 112410 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244683.1	NP_001231612.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 2
XM_017019421.1	XP_016874910.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
XM_017019420.1	XP_016874909.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
NM_000921.4	NP_000912.3	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1
XM_017019421.2	XP_016874910.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2

Pathways from BioSystems

Homologies

	PDE3A	XP_001096963.2
Canis lupus familiaris (dog)	PDE3A	XP_005637137.1
Rattus norvegicus (Norway rat)	Pde3a	NP_059033.1
Danio rerio (zebrafish)	pde3a	XP_695432.4
Pan troglodytes (chimpanzee)	PDE3A	XP_520783.2
Mus musculus (house mouse)	Pde3a	NP_061249.1
Gallus gallus (chicken)	PDE3A	XP_416416.4
Homo sapiens (human)	PDE3A	NP_000912.3
Bos taurus (cattle)	PDE3A	XP_002687798.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeting tumor cells based on Phosphodiesterase 3A expression.
Nazir M, Senkowski W, Nyberg F, Blom K, Edqvist PH, Jarvius M, Andersson C, Gustafsson MG, Nygren P, Larsson R, Frykn?s M
Experimental cell research361(2)308-315(2017 12)

PDE3A is hypermethylated in cisplatin resistant non-small cell lung cancer cells and is a modulator of chemotherapy response.
Tian FM, Zhong CY, Wang XN, Meng Y
European review for medical and pharmacological sciences21(11)2635-2641(2017 Jun)

cUMP hydrolysis by PDE3A.
Berrisch S, Ostermeyer J, Kaever V, K?lble S, Hilfiker-Kleiner D, Seifert R, Schneider EH
Naunyn-Schmiedeberg's archives of pharmacology390(3)269-280(2017 Mar)

Previously reported PDE3A-SLCO1C1 genetic variant does not correlate with anti-TNF response in a large UK rheumatoid arthritis cohort.
Smith SL, Plant D, Lee XH, Massey J, Hyrich K, Morgan AW, Wilson AG, Isaacs J, Barton A
Pharmacogenomics17(7)715-20(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vulnerability to antineoplastic phosphodiesterase inhibitors inhibitors has been associated with co-expression of PDE3A and SLFN12 in various tumor types.
Title: Targeting tumor cells based on Phosphodiesterase 3A expression.

PDE3A is a low-affinity and high-velocity enzyme for hydrolysis of cUMP in cardiac myocytes.
Title: cUMP hydrolysis by PDE3A.

We found a novel heterozygous missense mutation c.1336T>C in exon 4 of the PDE3A gene in a Japanese family with multiple HTNB patients. This mutation was found to be completely linked to the family members who inherited this condition.
Title: A PDE3A mutation in familial hypertension and brachydactyly syndrome.

The genetic variant, rs3794271, located within the PDE3A-SLCO1C1 locus was analyzed for correlation with treatment response using both the EULAR classification criteria and absolute change in (Delta)DAS28 scores as outcome measures correlated with anti-TNF response in a large UK rheumatoid arthritis cohort.
Title: Previously reported PDE3A-SLCO1C1 genetic variant does not correlate with anti-TNF response in a large UK rheumatoid arthritis cohort.

Chemical proteomics results show that besides known interactors such as 14-3-3 family proteins, PDE3A was found to associate with a PP2A complex composed of a regulatory, scaffold and catalytic subunit.
Title: Charting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach.

The following PDE3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25135	NM_000921.4 Latest version!	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu83854	XM_017019421.2 Latest version!	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu25277	NM_001244683.1 Latest version!	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu83853	XM_017019420.1 Latest version!	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu83854	XM_017019421.1	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE3A ( NM_000921.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000912.3 Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25135
Clone ID Related Accession (Same CDS sequence)	NM_000921.4
Accession Version	NM_000921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117369.1, AC112777.7, U36798.1, AK095203.1 and BQ024709.1. This sequence is a reference standard in the RefSeqGene project. On Sep 24, 2011 this sequence version replaced NM_000921.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117371.1, SRR1660807.152543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC CCGGCGACGC TGCACGAGTC AGGGACAAGC CCGTCCACAG TGGGGTGAGT 
CAAGCCCCCA CGGCGGGCCG GGACTGCCAC CATCGTGCGG ACCCCGCATC GCCGCGGGAC 
TCGGGCTGCC GTGGCTGCTG GGGAGACCTG GTGCTGCAGC CGCTCCGGAG CTCTCGGAAA 
CTTTCCTCCG CGCTGTGCGC GGGCTCCCTG TCCTTTCTGC TGGCGCTGCT GGTGAGGCTG 
GTCCGCGGGG AGGTCGGCTG TGACCTGGAG CAGTGTAAGG AGGCGGCGGC GGCGGAGGAG 
GAGGAAGCAG CCCCGGGAGC AGAAGGGGGC GTCTTCCCGG GGCCTCGGGG AGGTGCTCCC 
GGGGGCGGTG CGCGGCTCAG CCCCTGGCTG CAGCCCTCGG CGCTGCTCTT CAGTCTCCTG 
TGTGCCTTCT TCTGGATGGG CTTGTACCTC CTGCGCGCCG GGGTGCGCCT GCCTCTGGCT 
GTCGCGCTGC TGGCCGCCTG CTGCGGGGGG GAAGCGCTCG TCCAGATTGG GCTGGGCGTC 
GGGGAGGATC ACTTACTCTC ACTCCCCGCC GCGGGGGTGG TGCTCAGCTG CTTGGCCGCC 
GCGACATGGC TGGTGCTGAG GCTGAGGCTG GGCGTCCTCA TGATCGCCTT GACTAGCGCG 
GTCAGGACCG TGTCCCTCAT TTCCTTAGAG AGGTTCAAGG TCGCCTGGAG ACCTTACCTG 
GCGTACCTGG CCGGCGTGCT GGGGATCCTC TTGGCCAGGT ACGTGGAACA AATCTTGCCG 
CAGTCCGCGG AGGCGGCTCC AAGGGAGCAT TTGGGGTCCC AGCTGATTGC TGGGACCAAG 
GAAGATATCC CGGTGTTTAA GAGGAGGAGG CGGTCCAGCT CCGTCGTGTC CGCCGAGATG 
TCCGGCTGCA GCAGCAAGTC CCATCGGAGG ACCTCCCTGC CCTGTATACC GAGGGAACAG 
CTCATGGGGC ATTCAGAATG GGACCACAAA CGAGGGCCAA GAGGATCACA GTCTTCAGGA 
ACCAGTATTA CTGTGGACAT CGCCGTCATG GGCGAGGCCC ACGGCCTCAT TACCGACCTC 
CTGGCAGACC CTTCTCTTCC ACCAAACGTG TGCACATCCT TGAGAGCCGT GAGCAACTTG 
CTCAGCACAC AGCTCACCTT CCAGGCCATT CACAAGCCCA GAGTGAATCC CGTCACTTCG 
CTCAGTGAAA ACTATACCTG TTCTGACTCT GAAGAGAGCT CTGAAAAAGA CAAGCTTGCT 
ATTCCAAAGC GCCTGAGAAG GAGTTTGCCT CCTGGCTTGT TGAGACGAGT TTCTTCCACT 
TGGACCACCA CCACCTCGGC CACAGGTCTA CCCACCTTGG AGCCTGCACC AGTACGGAGA 
GACCGCAGCA CCAGCATCAA ACTGCAGGAA GCACCTTCAT CCAGTCCTGA TTCTTGGAAT 
AATCCAGTGA TGATGACCCT CACCAAAAGC AGATCCTTTA CTTCATCCTA TGCTATTTCT 
GCAGCTAACC ATGTAAAGGC TAAAAAGCAA AGTCGACCAG GTGCCCTCGC TAAAATTTCA 
CCTCTTTCAT CGCCCTGCTC CTCACCTCTC CAAGGGACTC CTGCCAGCAG CCTGGTCAGC 
AAAATTTCTG CAGTGCAGTT TCCAGAATCT GCTGACACAA CTGCCAAACA AAGCCTAGGT 
TCTCACAGGG CCTTAACTTA CACTCAGAGT GCCCCAGACC TATCCCCTCA AATCCTGACT 
CCACCTGTTA TATGTAGCAG CTGTGGCAGA CCATATTCCC AAGGGAATCC TGCTGATGAG 
CCCCTGGAGA GAAGTGGGGT AGCCACTCGG ACACCAAGTA GAACAGATGA CACTGCTCAA 
GTTACCTCTG ATTATGAAAC CAATAACAAC AGTGACAGCA GTGACATTGT ACAGAATGAA 
GATGAAACAG AGTGCCTGAG AGAGCCTCTG AGGAAAGCAT CGGCTTGCAG CACCTATGCT 
CCTGAGACCA TGATGTTTCT GGACAAACCA ATTCTTGCTC CCGAACCTCT TGTCATGGAT 
AACCTGGACT CAATTATGGA GCAGCTAAAT ACTTGGAATT TTCCAATTTT TGATTTAGTG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTTAGTCAGG TATCTTACAG ACTTTTTGAA 
GACATGGGCC TCTTTGAAGC TTTTAAAATT CCAATTAGGG AATTTATGAA TTATTTTCAT 
GCTTTGGAGA TTGGATATAG GGATATTCCT TATCATAACA GAATCCATGC CACTGATGTT 
TTACATGCTG TTTGGTATCT TACTACACAG CCTATTCCAG GCCTCTCAAC TGTGATTAAT 
GATCATGGTT CAACCAGTGA TTCAGATTCT GACAGTGGAT TTACACATGG ACATATGGGA 
TATGTATTCT CAAAAACGTA TAATGTGACA GATGATAAAT ACGGATGTCT GTCTGGGAAT 
ATCCCTGCCT TGGAGTTGAT GGCGCTGTAT GTGGCTGCAG CCATGCACGA TTATGATCAT 
CCAGGAAGGA CTAATGCTTT CCTGGTTGCA ACTAGTGCTC CTCAGGCGGT GCTATATAAC 
GATCGTTCAG TTTTGGAGAA TCATCACGCA GCTGCTGCAT GGAATCTTTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAAT TAACCTTGAC CATGTGGAAT TTAAGCATTT CCGTTTCCTT 
GTCATTGAAG CAATTTTGGC CACTGACCTG AAGAAACACT TTGACTTCGT AGCCAAATTT 
AATGGCAAGG TAAATGATGA TGTTGGAATA GATTGGACCA ATGAAAATGA TCGTCTACTG 
GTTTGTCAAA TGTGTATAAA GTTGGCTGAT ATCAATGGTC CAGCTAAATG TAAAGAACTC 
CATCTTCAGT GGACAGATGG TATTGTCAAT GAATTTTATG AACAGGGTGA TGAAGAGGCC 
AGCCTTGGAT TACCCATAAG CCCCTTCATG GATCGTTCTG CTCCTCAGCT GGCCAACCTT 
CAGGAATCCT TCATCTCTCA CATTGTGGGG CCTCTGTGCA ACTCCTATGA TTCAGCAGGA 
CTAATGCCTG GAAAATGGGT GGAAGACAGC GATGAGTCAG GAGATACTGA TGACCCAGAA 
GAAGAGGAGG AAGAAGCACC AGCACCAAAT GAAGAGGAAA CCTGTGAAAA TAATGAATCT 
CCAAAAAAGA AGACTTTCAA AAGGAGAAAA ATCTACTGCC AAATAACTCA GCACCTCTTA 
CAGAACCACA AGATGTGGAA GAAAGTCATT GAAGAGGAGC AACGGTTGGC AGGCATAGAA 
AATCAATCCC TGGACCAGAC CCCTCAGTCG CACTCTTCAG AACAGATCCA GGCTATCAAG 
GAAGAAGAAG AAGAGAAAGG GAAACCAAGA GGCGAGGAGA TACCAACCCA AAAGCCAGAC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000912.3
CDS	41..3466
Translation	MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSLSFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLLCAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRLGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

Target ORF information:

RefSeq Version	NM_000921.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000921.4

ORF Insert Sequence:

ATGGCAGTGC CCGGCGACGC TGCACGAGTC AGGGACAAGC CCGTCCACAG TGGGGTGAGT 
CAAGCCCCCA CGGCGGGCCG GGACTGCCAC CATCGTGCGG ACCCCGCATC GCCGCGGGAC 
TCGGGCTGCC GTGGCTGCTG GGGAGACCTG GTGCTGCAGC CGCTCCGGAG CTCTCGGAAA 
CTTTCCTCCG CGCTGTGCGC GGGCTCCCTG TCCTTTCTGC TGGCGCTGCT GGTGAGGCTG 
GTCCGCGGGG AGGTCGGCTG TGACCTGGAG CAGTGTAAGG AGGCGGCGGC GGCGGAGGAG 
GAGGAAGCAG CCCCGGGAGC AGAAGGGGGC GTCTTCCCGG GGCCTCGGGG AGGTGCTCCC 
GGGGGCGGTG CGCGGCTCAG CCCCTGGCTG CAGCCCTCGG CGCTGCTCTT CAGTCTCCTG 
TGTGCCTTCT TCTGGATGGG CTTGTACCTC CTGCGCGCCG GGGTGCGCCT GCCTCTGGCT 
GTCGCGCTGC TGGCCGCCTG CTGCGGGGGG GAAGCGCTCG TCCAGATTGG GCTGGGCGTC 
GGGGAGGATC ACTTACTCTC ACTCCCCGCC GCGGGGGTGG TGCTCAGCTG CTTGGCCGCC 
GCGACATGGC TGGTGCTGAG GCTGAGGCTG GGCGTCCTCA TGATCGCCTT GACTAGCGCG 
GTCAGGACCG TGTCCCTCAT TTCCTTAGAG AGGTTCAAGG TCGCCTGGAG ACCTTACCTG 
GCGTACCTGG CCGGCGTGCT GGGGATCCTC TTGGCCAGGT ACGTGGAACA AATCTTGCCG 
CAGTCCGCGG AGGCGGCTCC AAGGGAGCAT TTGGGGTCCC AGCTGATTGC TGGGACCAAG 
GAAGATATCC CGGTGTTTAA GAGGAGGAGG CGGTCCAGCT CCGTCGTGTC CGCCGAGATG 
TCCGGCTGCA GCAGCAAGTC CCATCGGAGG ACCTCCCTGC CCTGTATACC GAGGGAACAG 
CTCATGGGGC ATTCAGAATG GGACCACAAA CGAGGGCCAA GAGGATCACA GTCTTCAGGA 
ACCAGTATTA CTGTGGACAT CGCCGTCATG GGCGAGGCCC ACGGCCTCAT TACCGACCTC 
CTGGCAGACC CTTCTCTTCC ACCAAACGTG TGCACATCCT TGAGAGCCGT GAGCAACTTG 
CTCAGCACAC AGCTCACCTT CCAGGCCATT CACAAGCCCA GAGTGAATCC CGTCACTTCG 
CTCAGTGAAA ACTATACCTG TTCTGACTCT GAAGAGAGCT CTGAAAAAGA CAAGCTTGCT 
ATTCCAAAGC GCCTGAGAAG GAGTTTGCCT CCTGGCTTGT TGAGACGAGT TTCTTCCACT 
TGGACCACCA CCACCTCGGC CACAGGTCTA CCCACCTTGG AGCCTGCACC AGTACGGAGA 
GACCGCAGCA CCAGCATCAA ACTGCAGGAA GCACCTTCAT CCAGTCCTGA TTCTTGGAAT 
AATCCAGTGA TGATGACCCT CACCAAAAGC AGATCCTTTA CTTCATCCTA TGCTATTTCT 
GCAGCTAACC ATGTAAAGGC TAAAAAGCAA AGTCGACCAG GTGCCCTCGC TAAAATTTCA 
CCTCTTTCAT CGCCCTGCTC CTCACCTCTC CAAGGGACTC CTGCCAGCAG CCTGGTCAGC 
AAAATTTCTG CAGTGCAGTT TCCAGAATCT GCTGACACAA CTGCCAAACA AAGCCTAGGT 
TCTCACAGGG CCTTAACTTA CACTCAGAGT GCCCCAGACC TATCCCCTCA AATCCTGACT 
CCACCTGTTA TATGTAGCAG CTGTGGCAGA CCATATTCCC AAGGGAATCC TGCTGATGAG 
CCCCTGGAGA GAAGTGGGGT AGCCACTCGG ACACCAAGTA GAACAGATGA CACTGCTCAA 
GTTACCTCTG ATTATGAAAC CAATAACAAC AGTGACAGCA GTGACATTGT ACAGAATGAA 
GATGAAACAG AGTGCCTGAG AGAGCCTCTG AGGAAAGCAT CGGCTTGCAG CACCTATGCT 
CCTGAGACCA TGATGTTTCT GGACAAACCA ATTCTTGCTC CCGAACCTCT TGTCATGGAT 
AACCTGGACT CAATTATGGA GCAGCTAAAT ACTTGGAATT TTCCAATTTT TGATTTAGTG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTTAGTCAGG TATCTTACAG ACTTTTTGAA 
GACATGGGCC TCTTTGAAGC TTTTAAAATT CCAATTAGGG AATTTATGAA TTATTTTCAT 
GCTTTGGAGA TTGGATATAG GGATATTCCT TATCATAACA GAATCCATGC CACTGATGTT 
TTACATGCTG TTTGGTATCT TACTACACAG CCTATTCCAG GCCTCTCAAC TGTGATTAAT 
GATCATGGTT CAACCAGTGA TTCAGATTCT GACAGTGGAT TTACACATGG ACATATGGGA 
TATGTATTCT CAAAAACGTA TAATGTGACA GATGATAAAT ACGGATGTCT GTCTGGGAAT 
ATCCCTGCCT TGGAGTTGAT GGCGCTGTAT GTGGCTGCAG CCATGCACGA TTATGATCAT 
CCAGGAAGGA CTAATGCTTT CCTGGTTGCA ACTAGTGCTC CTCAGGCGGT GCTATATAAC 
GATCGTTCAG TTTTGGAGAA TCATCACGCA GCTGCTGCAT GGAATCTTTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAAT TAACCTTGAC CATGTGGAAT TTAAGCATTT CCGTTTCCTT 
GTCATTGAAG CAATTTTGGC CACTGACCTG AAGAAACACT TTGACTTCGT AGCCAAATTT 
AATGGCAAGG TAAATGATGA TGTTGGAATA GATTGGACCA ATGAAAATGA TCGTCTACTG 
GTTTGTCAAA TGTGTATAAA GTTGGCTGAT ATCAATGGTC CAGCTAAATG TAAAGAACTC 
CATCTTCAGT GGACAGATGG TATTGTCAAT GAATTTTATG AACAGGGTGA TGAAGAGGCC 
AGCCTTGGAT TACCCATAAG CCCCTTCATG GATCGTTCTG CTCCTCAGCT GGCCAACCTT 
CAGGAATCCT TCATCTCTCA CATTGTGGGG CCTCTGTGCA ACTCCTATGA TTCAGCAGGA 
CTAATGCCTG GAAAATGGGT GGAAGACAGC GATGAGTCAG GAGATACTGA TGACCCAGAA 
GAAGAGGAGG AAGAAGCACC AGCACCAAAT GAAGAGGAAA CCTGTGAAAA TAATGAATCT 
CCAAAAAAGA AGACTTTCAA AAGGAGAAAA ATCTACTGCC AAATAACTCA GCACCTCTTA 
CAGAACCACA AGATGTGGAA GAAAGTCATT GAAGAGGAGC AACGGTTGGC AGGCATAGAA 
AATCAATCCC TGGACCAGAC CCCTCAGTCG CACTCTTCAG AACAGATCCA GGCTATCAAG 
GAAGAAGAAG AAGAGAAAGG GAAACCAAGA GGCGAGGAGA TACCAACCCA AAAGCCAGAC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_017019421.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874910.1 Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83854
Clone ID Related Accession (Same CDS sequence)	XM_017019421.1 , XM_017019421.2
Accession Version	XM_017019421.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATT CAGAATGGGA CCACAAACGA GGGCCAAGAG GATCACAGTC TTCAGGAACC 
AGTATTACTG TGGACATCGC CGTCATGGGC GAGGCCCACG GCCTCATTAC CGACCTCCTG 
GCAGACCCTT CTCTTCCACC AAACGTGTGC ACATCCTTGA GAGCCGTGAG CAACTTGCTC 
AGCACACAGC TCACCTTCCA GGCCATTCAC AAGCCCAGAG TGAATCCCGT CACTTCGCTC 
AGTGAAAACT ATACCTGTTC TGACTCTGAA GAGAGCTCTG AAAAAGACAA GCTTGCTATT 
CCAAAGCGCC TGAGAAGGAG TTTGCCTCCT GGCTTGTTGA GACGAGTTTC TTCCACTTGG 
ACCACCACCA CCTCGGCCAC AGGTCTACCC ACCTTGGAGC CTGCACCAGT ACGGAGAGAC 
CGCAGCACCA GCATCAAACT GCAGGAAGCA CCTTCATCCA GTCCTGATTC TTGGAATAAT 
CCAGTGATGA TGACCCTCAC CAAAAGCAGA TCCTTTACTT CATCCTATGC TATTTCTGCA 
GCTAACCATG TAAAGGCTAA AAAGCAAAGT CGACCAGGTG CCCTCGCTAA AATTTCACCT 
CTTTCATCGC CCTGCTCCTC ACCTCTCCAA GGGACTCCTG CCAGCAGCCT GGTCAGCAAA 
ATTTCTGCAG TGCAGTTTCC AGAATCTGCT GACACAACTG CCAAACAAAG CCTAGGTTCT 
CACAGGGCCT TAACTTACAC TCAGAGTGCC CCAGACCTAT CCCCTCAAAT CCTGACTCCA 
CCTGTTATAT GTAGCAGCTG TGGCAGACCA TATTCCCAAG GGAATCCTGC TGATGAGCCC 
CTGGAGAGAA GTGGGGTAGC CACTCGGACA CCAAGTAGAA CAGATGACAC TGCTCAAGTT 
ACCTCTGATT ATGAAACCAA TAACAACAGT GACAGCAGTG ACATTGTACA GAATGAAGAT 
GAAACAGAGT GCCTGAGAGA GCCTCTGAGG AAAGCATCGG CTTGCAGCAC CTATGCTCCT 
GAGACCATGA TGTTTCTGGA CAAACCAATT CTTGCTCCCG AACCTCTTGT CATGGATAAC 
CTGGACTCAA TTATGGAGCA GCTAAATACT TGGAATTTTC CAATTTTTGA TTTAGTGGAA 
AATATAGGAA GAAAATGTGG CCGTATTCTT AGTCAGGTAT CTTACAGACT TTTTGAAGAC 
ATGGGCCTCT TTGAAGCTTT TAAAATTCCA ATTAGGGAAT TTATGAATTA TTTTCATGCT 
TTGGAGATTG GATATAGGGA TATTCCTTAT CATAACAGAA TCCATGCCAC TGATGTTTTA 
CATGCTGTTT GGTATCTTAC TACACAGCCT ATTCCAGGCC TCTCAACTGT GATTAATGAT 
CATGGTTCAA CCAGTGATTC AGATTCTGAC AGTGGATTTA CACATGGACA TATGGGATAT 
GTATTCTCAA AAACGTATAA TGTGACAGAT GATAAATACG GATGTCTGTC TGGGAATATC 
CCTGCCTTGG AGTTGATGGC GCTGTATGTG GCTGCAGCCA TGCACGATTA TGATCATCCA 
GGAAGGACTA ATGCTTTCCT GGTTGCAACT AGTGCTCCTC AGGCGGTGCT ATATAACGAT 
CGTTCAGTTT TGGAGAATCA TCACGCAGCT GCTGCATGGA ATCTTTTCAT GTCCCGGCCA 
GAGTATAACT TCTTAATTAA CCTTGACCAT GTGGAATTTA AGCATTTCCG TTTCCTTGTC 
ATTGAAGCAA TTTTGGCCAC TGACCTGAAG AAACACTTTG ACTTCGTAGC CAAATTTAAT 
GGCAAGGTAA ATGATGATGT TGGAATAGAT TGGACCAATG AAAATGATCG TCTACTGGTT 
TGTCAAATGT GTATAAAGTT GGCTGATATC AATGGTCCAG CTAAATGTAA AGAACTCCAT 
CTTCAGTGGA CAGATGGTAT TGTCAATGAA TTTTATGAAC AGGGTGATGA AGAGGCCAGC 
CTTGGATTAC CCATAAGCCC CTTCATGGAT CGTTCTGCTC CTCAGCTGGC CAACCTTCAG 
GAATCCTTCA TCTCTCACAT TGTGGGGCCT CTGTGCAACT CCTATGATTC AGCAGGACTA 
ATGCCTGGAA AATGGGTGGA AGACAGCGAT GAGTCAGGAG ATACTGATGA CCCAGAAGAA 
GAGGAGGAAG AAGCACCAGC ACCAAATGAA GAGGAAACCT GTGAAAATAA TGAATCTCCA 
AAAAAGAAGA CTTTCAAAAG GAGAAAAATC TACTGCCAAA TAACTCAGCA CCTCTTACAG 
AACCACAAGA TGTGGAAGAA AGTCATTGAA GAGGAGCAAC GGTTGGCAGG CATAGAAAAT 
CAATCCCTGG ACCAGACCCC TCAGTCGCAC TCTTCAGAAC AGATCCAGGC TATCAAGGAA 
GAAGAAGAAG AGAAAGGGAA ACCAAGAGGC GAGGAGATAC CAACCCAAAA GCCAGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874910.1
CDS	3702..6164
Translation	MGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

Target ORF information:

RefSeq Version	XM_017019421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019421.1

ORF Insert Sequence:

ATGGGGCATT CAGAATGGGA CCACAAACGA GGGCCAAGAG GATCACAGTC TTCAGGAACC 
AGTATTACTG TGGACATCGC CGTCATGGGC GAGGCCCACG GCCTCATTAC CGACCTCCTG 
GCAGACCCTT CTCTTCCACC AAACGTGTGC ACATCCTTGA GAGCCGTGAG CAACTTGCTC 
AGCACACAGC TCACCTTCCA GGCCATTCAC AAGCCCAGAG TGAATCCCGT CACTTCGCTC 
AGTGAAAACT ATACCTGTTC TGACTCTGAA GAGAGCTCTG AAAAAGACAA GCTTGCTATT 
CCAAAGCGCC TGAGAAGGAG TTTGCCTCCT GGCTTGTTGA GACGAGTTTC TTCCACTTGG 
ACCACCACCA CCTCGGCCAC AGGTCTACCC ACCTTGGAGC CTGCACCAGT ACGGAGAGAC 
CGCAGCACCA GCATCAAACT GCAGGAAGCA CCTTCATCCA GTCCTGATTC TTGGAATAAT 
CCAGTGATGA TGACCCTCAC CAAAAGCAGA TCCTTTACTT CATCCTATGC TATTTCTGCA 
GCTAACCATG TAAAGGCTAA AAAGCAAAGT CGACCAGGTG CCCTCGCTAA AATTTCACCT 
CTTTCATCGC CCTGCTCCTC ACCTCTCCAA GGGACTCCTG CCAGCAGCCT GGTCAGCAAA 
ATTTCTGCAG TGCAGTTTCC AGAATCTGCT GACACAACTG CCAAACAAAG CCTAGGTTCT 
CACAGGGCCT TAACTTACAC TCAGAGTGCC CCAGACCTAT CCCCTCAAAT CCTGACTCCA 
CCTGTTATAT GTAGCAGCTG TGGCAGACCA TATTCCCAAG GGAATCCTGC TGATGAGCCC 
CTGGAGAGAA GTGGGGTAGC CACTCGGACA CCAAGTAGAA CAGATGACAC TGCTCAAGTT 
ACCTCTGATT ATGAAACCAA TAACAACAGT GACAGCAGTG ACATTGTACA GAATGAAGAT 
GAAACAGAGT GCCTGAGAGA GCCTCTGAGG AAAGCATCGG CTTGCAGCAC CTATGCTCCT 
GAGACCATGA TGTTTCTGGA CAAACCAATT CTTGCTCCCG AACCTCTTGT CATGGATAAC 
CTGGACTCAA TTATGGAGCA GCTAAATACT TGGAATTTTC CAATTTTTGA TTTAGTGGAA 
AATATAGGAA GAAAATGTGG CCGTATTCTT AGTCAGGTAT CTTACAGACT TTTTGAAGAC 
ATGGGCCTCT TTGAAGCTTT TAAAATTCCA ATTAGGGAAT TTATGAATTA TTTTCATGCT 
TTGGAGATTG GATATAGGGA TATTCCTTAT CATAACAGAA TCCATGCCAC TGATGTTTTA 
CATGCTGTTT GGTATCTTAC TACACAGCCT ATTCCAGGCC TCTCAACTGT GATTAATGAT 
CATGGTTCAA CCAGTGATTC AGATTCTGAC AGTGGATTTA CACATGGACA TATGGGATAT 
GTATTCTCAA AAACGTATAA TGTGACAGAT GATAAATACG GATGTCTGTC TGGGAATATC 
CCTGCCTTGG AGTTGATGGC GCTGTATGTG GCTGCAGCCA TGCACGATTA TGATCATCCA 
GGAAGGACTA ATGCTTTCCT GGTTGCAACT AGTGCTCCTC AGGCGGTGCT ATATAACGAT 
CGTTCAGTTT TGGAGAATCA TCACGCAGCT GCTGCATGGA ATCTTTTCAT GTCCCGGCCA 
GAGTATAACT TCTTAATTAA CCTTGACCAT GTGGAATTTA AGCATTTCCG TTTCCTTGTC 
ATTGAAGCAA TTTTGGCCAC TGACCTGAAG AAACACTTTG ACTTCGTAGC CAAATTTAAT 
GGCAAGGTAA ATGATGATGT TGGAATAGAT TGGACCAATG AAAATGATCG TCTACTGGTT 
TGTCAAATGT GTATAAAGTT GGCTGATATC AATGGTCCAG CTAAATGTAA AGAACTCCAT 
CTTCAGTGGA CAGATGGTAT TGTCAATGAA TTTTATGAAC AGGGTGATGA AGAGGCCAGC 
CTTGGATTAC CCATAAGCCC CTTCATGGAT CGTTCTGCTC CTCAGCTGGC CAACCTTCAG 
GAATCCTTCA TCTCTCACAT TGTGGGGCCT CTGTGCAACT CCTATGATTC AGCAGGACTA 
ATGCCTGGAA AATGGGTGGA AGACAGCGAT GAGTCAGGAG ATACTGATGA CCCAGAAGAA 
GAGGAGGAAG AAGCACCAGC ACCAAATGAA GAGGAAACCT GTGAAAATAA TGAATCTCCA 
AAAAAGAAGA CTTTCAAAAG GAGAAAAATC TACTGCCAAA TAACTCAGCA CCTCTTACAG 
AACCACAAGA TGTGGAAGAA AGTCATTGAA GAGGAGCAAC GGTTGGCAGG CATAGAAAAT 
CAATCCCTGG ACCAGACCCC TCAGTCGCAC TCTTCAGAAC AGATCCAGGC TATCAAGGAA 
GAAGAAGAAG AGAAAGGGAA ACCAAGAGGC GAGGAGATAC CAACCCAAAA GCCAGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_017019421.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874910.1 Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.

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CloneID	OHu83854
Clone ID Related Accession (Same CDS sequence)	XM_017019421.1 , XM_017019421.2
Accession Version	XM_017019421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATT CAGAATGGGA CCACAAACGA GGGCCAAGAG GATCACAGTC TTCAGGAACC 
AGTATTACTG TGGACATCGC CGTCATGGGC GAGGCCCACG GCCTCATTAC CGACCTCCTG 
GCAGACCCTT CTCTTCCACC AAACGTGTGC ACATCCTTGA GAGCCGTGAG CAACTTGCTC 
AGCACACAGC TCACCTTCCA GGCCATTCAC AAGCCCAGAG TGAATCCCGT CACTTCGCTC 
AGTGAAAACT ATACCTGTTC TGACTCTGAA GAGAGCTCTG AAAAAGACAA GCTTGCTATT 
CCAAAGCGCC TGAGAAGGAG TTTGCCTCCT GGCTTGTTGA GACGAGTTTC TTCCACTTGG 
ACCACCACCA CCTCGGCCAC AGGTCTACCC ACCTTGGAGC CTGCACCAGT ACGGAGAGAC 
CGCAGCACCA GCATCAAACT GCAGGAAGCA CCTTCATCCA GTCCTGATTC TTGGAATAAT 
CCAGTGATGA TGACCCTCAC CAAAAGCAGA TCCTTTACTT CATCCTATGC TATTTCTGCA 
GCTAACCATG TAAAGGCTAA AAAGCAAAGT CGACCAGGTG CCCTCGCTAA AATTTCACCT 
CTTTCATCGC CCTGCTCCTC ACCTCTCCAA GGGACTCCTG CCAGCAGCCT GGTCAGCAAA 
ATTTCTGCAG TGCAGTTTCC AGAATCTGCT GACACAACTG CCAAACAAAG CCTAGGTTCT 
CACAGGGCCT TAACTTACAC TCAGAGTGCC CCAGACCTAT CCCCTCAAAT CCTGACTCCA 
CCTGTTATAT GTAGCAGCTG TGGCAGACCA TATTCCCAAG GGAATCCTGC TGATGAGCCC 
CTGGAGAGAA GTGGGGTAGC CACTCGGACA CCAAGTAGAA CAGATGACAC TGCTCAAGTT 
ACCTCTGATT ATGAAACCAA TAACAACAGT GACAGCAGTG ACATTGTACA GAATGAAGAT 
GAAACAGAGT GCCTGAGAGA GCCTCTGAGG AAAGCATCGG CTTGCAGCAC CTATGCTCCT 
GAGACCATGA TGTTTCTGGA CAAACCAATT CTTGCTCCCG AACCTCTTGT CATGGATAAC 
CTGGACTCAA TTATGGAGCA GCTAAATACT TGGAATTTTC CAATTTTTGA TTTAGTGGAA 
AATATAGGAA GAAAATGTGG CCGTATTCTT AGTCAGGTAT CTTACAGACT TTTTGAAGAC 
ATGGGCCTCT TTGAAGCTTT TAAAATTCCA ATTAGGGAAT TTATGAATTA TTTTCATGCT 
TTGGAGATTG GATATAGGGA TATTCCTTAT CATAACAGAA TCCATGCCAC TGATGTTTTA 
CATGCTGTTT GGTATCTTAC TACACAGCCT ATTCCAGGCC TCTCAACTGT GATTAATGAT 
CATGGTTCAA CCAGTGATTC AGATTCTGAC AGTGGATTTA CACATGGACA TATGGGATAT 
GTATTCTCAA AAACGTATAA TGTGACAGAT GATAAATACG GATGTCTGTC TGGGAATATC 
CCTGCCTTGG AGTTGATGGC GCTGTATGTG GCTGCAGCCA TGCACGATTA TGATCATCCA 
GGAAGGACTA ATGCTTTCCT GGTTGCAACT AGTGCTCCTC AGGCGGTGCT ATATAACGAT 
CGTTCAGTTT TGGAGAATCA TCACGCAGCT GCTGCATGGA ATCTTTTCAT GTCCCGGCCA 
GAGTATAACT TCTTAATTAA CCTTGACCAT GTGGAATTTA AGCATTTCCG TTTCCTTGTC 
ATTGAAGCAA TTTTGGCCAC TGACCTGAAG AAACACTTTG ACTTCGTAGC CAAATTTAAT 
GGCAAGGTAA ATGATGATGT TGGAATAGAT TGGACCAATG AAAATGATCG TCTACTGGTT 
TGTCAAATGT GTATAAAGTT GGCTGATATC AATGGTCCAG CTAAATGTAA AGAACTCCAT 
CTTCAGTGGA CAGATGGTAT TGTCAATGAA TTTTATGAAC AGGGTGATGA AGAGGCCAGC 
CTTGGATTAC CCATAAGCCC CTTCATGGAT CGTTCTGCTC CTCAGCTGGC CAACCTTCAG 
GAATCCTTCA TCTCTCACAT TGTGGGGCCT CTGTGCAACT CCTATGATTC AGCAGGACTA 
ATGCCTGGAA AATGGGTGGA AGACAGCGAT GAGTCAGGAG ATACTGATGA CCCAGAAGAA 
GAGGAGGAAG AAGCACCAGC ACCAAATGAA GAGGAAACCT GTGAAAATAA TGAATCTCCA 
AAAAAGAAGA CTTTCAAAAG GAGAAAAATC TACTGCCAAA TAACTCAGCA CCTCTTACAG 
AACCACAAGA TGTGGAAGAA AGTCATTGAA GAGGAGCAAC GGTTGGCAGG CATAGAAAAT 
CAATCCCTGG ACCAGACCCC TCAGTCGCAC TCTTCAGAAC AGATCCAGGC TATCAAGGAA 
GAAGAAGAAG AGAAAGGGAA ACCAAGAGGC GAGGAGATAC CAACCCAAAA GCCAGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874910.1
CDS	3772..6234
Translation	MGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

Target ORF information:

RefSeq Version	XM_017019421.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019421.2

ORF Insert Sequence:

ATGGGGCATT CAGAATGGGA CCACAAACGA GGGCCAAGAG GATCACAGTC TTCAGGAACC 
AGTATTACTG TGGACATCGC CGTCATGGGC GAGGCCCACG GCCTCATTAC CGACCTCCTG 
GCAGACCCTT CTCTTCCACC AAACGTGTGC ACATCCTTGA GAGCCGTGAG CAACTTGCTC 
AGCACACAGC TCACCTTCCA GGCCATTCAC AAGCCCAGAG TGAATCCCGT CACTTCGCTC 
AGTGAAAACT ATACCTGTTC TGACTCTGAA GAGAGCTCTG AAAAAGACAA GCTTGCTATT 
CCAAAGCGCC TGAGAAGGAG TTTGCCTCCT GGCTTGTTGA GACGAGTTTC TTCCACTTGG 
ACCACCACCA CCTCGGCCAC AGGTCTACCC ACCTTGGAGC CTGCACCAGT ACGGAGAGAC 
CGCAGCACCA GCATCAAACT GCAGGAAGCA CCTTCATCCA GTCCTGATTC TTGGAATAAT 
CCAGTGATGA TGACCCTCAC CAAAAGCAGA TCCTTTACTT CATCCTATGC TATTTCTGCA 
GCTAACCATG TAAAGGCTAA AAAGCAAAGT CGACCAGGTG CCCTCGCTAA AATTTCACCT 
CTTTCATCGC CCTGCTCCTC ACCTCTCCAA GGGACTCCTG CCAGCAGCCT GGTCAGCAAA 
ATTTCTGCAG TGCAGTTTCC AGAATCTGCT GACACAACTG CCAAACAAAG CCTAGGTTCT 
CACAGGGCCT TAACTTACAC TCAGAGTGCC CCAGACCTAT CCCCTCAAAT CCTGACTCCA 
CCTGTTATAT GTAGCAGCTG TGGCAGACCA TATTCCCAAG GGAATCCTGC TGATGAGCCC 
CTGGAGAGAA GTGGGGTAGC CACTCGGACA CCAAGTAGAA CAGATGACAC TGCTCAAGTT 
ACCTCTGATT ATGAAACCAA TAACAACAGT GACAGCAGTG ACATTGTACA GAATGAAGAT 
GAAACAGAGT GCCTGAGAGA GCCTCTGAGG AAAGCATCGG CTTGCAGCAC CTATGCTCCT 
GAGACCATGA TGTTTCTGGA CAAACCAATT CTTGCTCCCG AACCTCTTGT CATGGATAAC 
CTGGACTCAA TTATGGAGCA GCTAAATACT TGGAATTTTC CAATTTTTGA TTTAGTGGAA 
AATATAGGAA GAAAATGTGG CCGTATTCTT AGTCAGGTAT CTTACAGACT TTTTGAAGAC 
ATGGGCCTCT TTGAAGCTTT TAAAATTCCA ATTAGGGAAT TTATGAATTA TTTTCATGCT 
TTGGAGATTG GATATAGGGA TATTCCTTAT CATAACAGAA TCCATGCCAC TGATGTTTTA 
CATGCTGTTT GGTATCTTAC TACACAGCCT ATTCCAGGCC TCTCAACTGT GATTAATGAT 
CATGGTTCAA CCAGTGATTC AGATTCTGAC AGTGGATTTA CACATGGACA TATGGGATAT 
GTATTCTCAA AAACGTATAA TGTGACAGAT GATAAATACG GATGTCTGTC TGGGAATATC 
CCTGCCTTGG AGTTGATGGC GCTGTATGTG GCTGCAGCCA TGCACGATTA TGATCATCCA 
GGAAGGACTA ATGCTTTCCT GGTTGCAACT AGTGCTCCTC AGGCGGTGCT ATATAACGAT 
CGTTCAGTTT TGGAGAATCA TCACGCAGCT GCTGCATGGA ATCTTTTCAT GTCCCGGCCA 
GAGTATAACT TCTTAATTAA CCTTGACCAT GTGGAATTTA AGCATTTCCG TTTCCTTGTC 
ATTGAAGCAA TTTTGGCCAC TGACCTGAAG AAACACTTTG ACTTCGTAGC CAAATTTAAT 
GGCAAGGTAA ATGATGATGT TGGAATAGAT TGGACCAATG AAAATGATCG TCTACTGGTT 
TGTCAAATGT GTATAAAGTT GGCTGATATC AATGGTCCAG CTAAATGTAA AGAACTCCAT 
CTTCAGTGGA CAGATGGTAT TGTCAATGAA TTTTATGAAC AGGGTGATGA AGAGGCCAGC 
CTTGGATTAC CCATAAGCCC CTTCATGGAT CGTTCTGCTC CTCAGCTGGC CAACCTTCAG 
GAATCCTTCA TCTCTCACAT TGTGGGGCCT CTGTGCAACT CCTATGATTC AGCAGGACTA 
ATGCCTGGAA AATGGGTGGA AGACAGCGAT GAGTCAGGAG ATACTGATGA CCCAGAAGAA 
GAGGAGGAAG AAGCACCAGC ACCAAATGAA GAGGAAACCT GTGAAAATAA TGAATCTCCA 
AAAAAGAAGA CTTTCAAAAG GAGAAAAATC TACTGCCAAA TAACTCAGCA CCTCTTACAG 
AACCACAAGA TGTGGAAGAA AGTCATTGAA GAGGAGCAAC GGTTGGCAGG CATAGAAAAT 
CAATCCCTGG ACCAGACCCC TCAGTCGCAC TCTTCAGAAC AGATCCAGGC TATCAAGGAA 
GAAGAAGAAG AGAAAGGGAA ACCAAGAGGC GAGGAGATAC CAACCCAAAA GCCAGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( NM_001244683.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231612.1 Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 2, mRNA.

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CloneID	OHu25277
Clone ID Related Accession (Same CDS sequence)	NM_001244683.1
Accession Version	NM_001244683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK308317.1, AC112777.7, BQ187047.1, AK095203.1 and BQ024709.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308317.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACGA TATTTTCCAA ATCGTGGAGC TTCTACTGGG AAAAGTCTTC AGGAACCAGT 
ATTACTGTGG ACATCGCCGT CATGGGCGAG GCCCACGGCC TCATTACCGA CCTCCTGGCA 
GACCCTTCTC TTCCACCAAA CGTGTGCACA TCCTTGAGAG CCGTGAGCAA CTTGCTCAGC 
ACACAGCTCA CCTTCCAGGC CATTCACAAG CCCAGAGTGA ATCCCGTCAC TTCGCTCAGT 
GAAAACTATA CCTGTTCTGA CTCTGAAGAG AGCTCTGAAA AAGACAAGCT TGCTATTCCA 
AAGCGCCTGA GAAGGAGTTT GCCTCCTGGC TTGTTGAGAC GAGTTTCTTC CACTTGGACC 
ACCACCACCT CGGCCACAGG TCTACCCACC TTGGAGCCTG CACCAGTACG GAGAGACCGC 
AGCACCAGCA TCAAACTGCA GGAAGCACCT TCATCCAGTC CTGATTCTTG GAATAATCCA 
GTGATGATGA CCCTCACCAA AAGCAGATCC TTTACTTCAT CCTATGCTAT TTCTGCAGCT 
AACCATGTAA AGGCTAAAAA GCAAAGTCGA CCAGGTGCCC TCGCTAAAAT TTCACCTCTT 
TCATCGCCCT GCTCCTCACC TCTCCAAGGG ACTCCTGCCA GCAGCCTGGT CAGCAAAATT 
TCTGCAGTGC AGTTTCCAGA ATCTGCTGAC ACAACTGCCA AACAAAGCCT AGGTTCTCAC 
AGGGCCTTAA CTTACACTCA GAGTGCCCCA GACCTATCCC CTCAAATCCT GACTCCACCT 
GTTATATGTA GCAGCTGTGG CAGACCATAT TCCCAAGGGA ATCCTGCTGA TGAGCCCCTG 
GAGAGAAGTG GGGTAGCCAC TCGGACACCA AGTAGAACAG ATGACACTGC TCAAGTTACC 
TCTGATTATG AAACCAATAA CAACAGTGAC AGCAGTGACA TTGTACAGAA TGAAGATGAA 
ACAGAGTGCC TGAGAGAGCC TCTGAGGAAA GCATCGGCTT GCAGCACCTA TGCTCCTGAG 
ACCATGATGT TTCTGGACAA ACCAATTCTT GCTCCCGAAC CTCTTGTCAT GGATAACCTG 
GACTCAATTA TGGAGCAGCT AAATACTTGG AATTTTCCAA TTTTTGATTT AGTGGAAAAT 
ATAGGAAGAA AATGTGGCCG TATTCTTAGT CAGGTATCTT ACAGACTTTT TGAAGACATG 
GGCCTCTTTG AAGCTTTTAA AATTCCAATT AGGGAATTTA TGAATTATTT TCATGCTTTG 
GAGATTGGAT ATAGGGATAT TCCTTATCAT AACAGAATCC ATGCCACTGA TGTTTTACAT 
GCTGTTTGGT ATCTTACTAC ACAGCCTATT CCAGGCCTCT CAACTGTGAT TAATGATCAT 
GGTTCAACCA GTGATTCAGA TTCTGACAGT GGATTTACAC ATGGACATAT GGGATATGTA 
TTCTCAAAAA CGTATAATGT GACAGATGAT AAATACGGAT GTCTGTCTGG GAATATCCCT 
GCCTTGGAGT TGATGGCGCT GTATGTGGCT GCAGCCATGC ACGATTATGA TCATCCAGGA 
AGGACTAATG CTTTCCTGGT TGCAACTAGT GCTCCTCAGG CGGTGCTATA TAACGATCGT 
TCAGTTTTGG AGAATCATCA CGCAGCTGCT GCATGGAATC TTTTCATGTC CCGGCCAGAG 
TATAACTTCT TAATTAACCT TGACCATGTG GAATTTAAGC ATTTCCGTTT CCTTGTCATT 
GAAGCAATTT TGGCCACTGA CCTGAAGAAA CACTTTGACT TCGTAGCCAA ATTTAATGGC 
AAGGTAAATG ATGATGTTGG AATAGATTGG ACCAATGAAA ATGATCGTCT ACTGGTTTGT 
CAAATGTGTA TAAAGTTGGC TGATATCAAT GGTCCAGCTA AATGTAAAGA ACTCCATCTT 
CAGTGGACAG ATGGTATTGT CAATGAATTT TATGAACAGG GTGATGAAGA GGCCAGCCTT 
GGATTACCCA TAAGCCCCTT CATGGATCGT TCTGCTCCTC AGCTGGCCAA CCTTCAGGAA 
TCCTTCATCT CTCACATTGT GGGGCCTCTG TGCAACTCCT ATGATTCAGC AGGACTAATG 
CCTGGAAAAT GGGTGGAAGA CAGCGATGAG TCAGGAGATA CTGATGACCC AGAAGAAGAG 
GAGGAAGAAG CACCAGCACC AAATGAAGAG GAAACCTGTG AAAATAATGA ATCTCCAAAA 
AAGAAGACTT TCAAAAGGAG AAAAATCTAC TGCCAAATAA CTCAGCACCT CTTACAGAAC 
CACAAGATGT GGAAGAAAGT CATTGAAGAG GAGCAACGGT TGGCAGGCAT AGAAAATCAA 
TCCCTGGACC AGACCCCTCA GTCGCACTCT TCAGAACAGA TCCAGGCTAT CAAGGAAGAA 
GAAGAAGAGA AAGGGAAACC AAGAGGCGAG GAGATACCAA CCCAAAAGCC AGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231612.1
CDS	264..2723
Translation	MVTIFSKSWSFYWEKSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

Target ORF information:

RefSeq Version	NM_001244683.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244683.1

ORF Insert Sequence:

ATGGTGACGA TATTTTCCAA ATCGTGGAGC TTCTACTGGG AAAAGTCTTC AGGAACCAGT 
ATTACTGTGG ACATCGCCGT CATGGGCGAG GCCCACGGCC TCATTACCGA CCTCCTGGCA 
GACCCTTCTC TTCCACCAAA CGTGTGCACA TCCTTGAGAG CCGTGAGCAA CTTGCTCAGC 
ACACAGCTCA CCTTCCAGGC CATTCACAAG CCCAGAGTGA ATCCCGTCAC TTCGCTCAGT 
GAAAACTATA CCTGTTCTGA CTCTGAAGAG AGCTCTGAAA AAGACAAGCT TGCTATTCCA 
AAGCGCCTGA GAAGGAGTTT GCCTCCTGGC TTGTTGAGAC GAGTTTCTTC CACTTGGACC 
ACCACCACCT CGGCCACAGG TCTACCCACC TTGGAGCCTG CACCAGTACG GAGAGACCGC 
AGCACCAGCA TCAAACTGCA GGAAGCACCT TCATCCAGTC CTGATTCTTG GAATAATCCA 
GTGATGATGA CCCTCACCAA AAGCAGATCC TTTACTTCAT CCTATGCTAT TTCTGCAGCT 
AACCATGTAA AGGCTAAAAA GCAAAGTCGA CCAGGTGCCC TCGCTAAAAT TTCACCTCTT 
TCATCGCCCT GCTCCTCACC TCTCCAAGGG ACTCCTGCCA GCAGCCTGGT CAGCAAAATT 
TCTGCAGTGC AGTTTCCAGA ATCTGCTGAC ACAACTGCCA AACAAAGCCT AGGTTCTCAC 
AGGGCCTTAA CTTACACTCA GAGTGCCCCA GACCTATCCC CTCAAATCCT GACTCCACCT 
GTTATATGTA GCAGCTGTGG CAGACCATAT TCCCAAGGGA ATCCTGCTGA TGAGCCCCTG 
GAGAGAAGTG GGGTAGCCAC TCGGACACCA AGTAGAACAG ATGACACTGC TCAAGTTACC 
TCTGATTATG AAACCAATAA CAACAGTGAC AGCAGTGACA TTGTACAGAA TGAAGATGAA 
ACAGAGTGCC TGAGAGAGCC TCTGAGGAAA GCATCGGCTT GCAGCACCTA TGCTCCTGAG 
ACCATGATGT TTCTGGACAA ACCAATTCTT GCTCCCGAAC CTCTTGTCAT GGATAACCTG 
GACTCAATTA TGGAGCAGCT AAATACTTGG AATTTTCCAA TTTTTGATTT AGTGGAAAAT 
ATAGGAAGAA AATGTGGCCG TATTCTTAGT CAGGTATCTT ACAGACTTTT TGAAGACATG 
GGCCTCTTTG AAGCTTTTAA AATTCCAATT AGGGAATTTA TGAATTATTT TCATGCTTTG 
GAGATTGGAT ATAGGGATAT TCCTTATCAT AACAGAATCC ATGCCACTGA TGTTTTACAT 
GCTGTTTGGT ATCTTACTAC ACAGCCTATT CCAGGCCTCT CAACTGTGAT TAATGATCAT 
GGTTCAACCA GTGATTCAGA TTCTGACAGT GGATTTACAC ATGGACATAT GGGATATGTA 
TTCTCAAAAA CGTATAATGT GACAGATGAT AAATACGGAT GTCTGTCTGG GAATATCCCT 
GCCTTGGAGT TGATGGCGCT GTATGTGGCT GCAGCCATGC ACGATTATGA TCATCCAGGA 
AGGACTAATG CTTTCCTGGT TGCAACTAGT GCTCCTCAGG CGGTGCTATA TAACGATCGT 
TCAGTTTTGG AGAATCATCA CGCAGCTGCT GCATGGAATC TTTTCATGTC CCGGCCAGAG 
TATAACTTCT TAATTAACCT TGACCATGTG GAATTTAAGC ATTTCCGTTT CCTTGTCATT 
GAAGCAATTT TGGCCACTGA CCTGAAGAAA CACTTTGACT TCGTAGCCAA ATTTAATGGC 
AAGGTAAATG ATGATGTTGG AATAGATTGG ACCAATGAAA ATGATCGTCT ACTGGTTTGT 
CAAATGTGTA TAAAGTTGGC TGATATCAAT GGTCCAGCTA AATGTAAAGA ACTCCATCTT 
CAGTGGACAG ATGGTATTGT CAATGAATTT TATGAACAGG GTGATGAAGA GGCCAGCCTT 
GGATTACCCA TAAGCCCCTT CATGGATCGT TCTGCTCCTC AGCTGGCCAA CCTTCAGGAA 
TCCTTCATCT CTCACATTGT GGGGCCTCTG TGCAACTCCT ATGATTCAGC AGGACTAATG 
CCTGGAAAAT GGGTGGAAGA CAGCGATGAG TCAGGAGATA CTGATGACCC AGAAGAAGAG 
GAGGAAGAAG CACCAGCACC AAATGAAGAG GAAACCTGTG AAAATAATGA ATCTCCAAAA 
AAGAAGACTT TCAAAAGGAG AAAAATCTAC TGCCAAATAA CTCAGCACCT CTTACAGAAC 
CACAAGATGT GGAAGAAAGT CATTGAAGAG GAGCAACGGT TGGCAGGCAT AGAAAATCAA 
TCCCTGGACC AGACCCCTCA GTCGCACTCT TCAGAACAGA TCCAGGCTAT CAAGGAAGAA 
GAAGAAGAGA AAGGGAAACC AAGAGGCGAG GAGATACCAA CCCAAAAGCC AGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_017019420.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874909.1 Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X1, mRNA.

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CloneID	OHu83853
Clone ID Related Accession (Same CDS sequence)	XM_017019420.1
Accession Version	XM_017019420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CCGGCGACGC TGCACGAGTC AGGGACAAGC CCGTCCACAG TGGGGTGAGT 
CAAGCCCCCA CGGCGGGCCG GGACTGCCAC CATCGTGCGG ACCCCGCATC GCCGCGGGAC 
TCGGGCTGCC GTGGCTGCTG GGGAGACCTG GTGCTGCAGC CGCTCCGGAG CTCTCGGAAA 
CTTTCCTCCG CGCTGTGCGC GGGCTCCCTG TCCTTTCTGC TGGCGCTGCT GGTGAGGCTG 
GTCCGCGGGG AGGTCGGCTG TGACCTGGAG CAGTGTAAGG AGGCGGCGGC GGCGGAGGAG 
GAGGAAGCAG CCCCGGGAGC AGAAGGGGGC GTCTTCCCGG GGCCTCGGGG AGGTGCTCCC 
GGGGGCGGTG CGCGGCTCAG CCCCTGGCTG CAGCCCTCGG CGCTGCTCTT CAGTCTCCTG 
TGTGCCTTCT TCTGGATGGG CTTGTACCTC CTGCGCGCCG GGGTGCGCCT GCCTCTGGCT 
GTCGCGCTGC TGGCCGCCTG CTGCGGGGGG GAAGCGCTCG TCCAGATTGG GCTGGGCGTC 
GGGGAGGATC ACTTACTCTC ACTCCCCGCC GCGGGGGTGG TGCTCAGCTG CTTGGCCGCC 
GCGACATGGC TGGTGCTGAG GCTGAGGCTG GGCGTCCTCA TGATCGCCTT GACTAGCGCG 
GTCAGGACCG TGTCCCTCAT TTCCTTAGAG AGGTTCAAGG TCGCCTGGAG ACCTTACCTG 
GCGTACCTGG CCGGCGTGCT GGGGATCCTC TTGGCCAGGT ACGTGGAACA AATCTTGCCG 
CAGTCCGCGG AGGCGGCTCC AAGGGAGCAT TTGGGGTCCC AGCTGATTGC TGGGACCAAG 
GAAGATATCC CGGTGTTTAA GAGGAGGAGG CGGTCCAGCT CCGTCGTGTC CGCCGAGATG 
TCCGGCTGCA GCAGCAAGTC CCATCGGAGG ACCTCCCTGC CCTGTATACC GAGGGAACAG 
CTCATGGGGC ATTCAGAATG GGACCACAAA CGAGGGCCAA GAGGATCACA GTCTTCAGGA 
ACCAGTATTA CTGTGGACAT CGCCGTCATG GGCGAGGCCC ACGGCCTCAT TACCGACCTC 
CTGGCAGACC CTTCTCTTCC ACCAAACGTG TGCACATCCT TGAGAGCCGT GAGCAACTTG 
CTCAGCACAC AGCTCACCTT CCAGGCCATT CACAAGCCCA GAGTGAATCC CGTCACTTCG 
CTCAGTGAAA ACTATACCTG TTCTGACTCT GAAGAGAGCT CTGAAAAAGA CAAGCTTGCT 
ATTCCAAAGC GCCTGAGAAG GAGTTTGCCT CCTGGCTTGT TGAGACGAGT TTCTTCCACT 
TGGACCACCA CCACCTCGGC CACAGGTCTA CCCACCTTGG AGCCTGCACC AGTACGGAGA 
GACCGCAGCA CCAGCATCAA ACTGCAGGAA GCACCTTCAT CCAGTCCTGA TTCTTGGAAT 
AATCCAGTGA TGATGACCCT CACCAAAAGC AGATCCTTTA CTTCATCCTA TGCTATTTCT 
GCAGCTAACC ATGTAAAGGC TAAAAAGCAA AGTCGACCAG GTGCCCTCGC TAAAATTTCA 
CCTCTTTCAT CGCCCTGCTC CTCACCTCTC CAAGGGACTC CTGCCAGCAG CCTGGTCAGC 
AAAATTTCTG CAGTGCAGTT TCCAGAATCT GCTGACACAA CTGCCAAACA AAGCCTAGGT 
TCTCACAGGG CCTTAACTTA CACTCAGAGT GCCCCAGACC TATCCCCTCA AATCCTGACT 
CCACCTGTTA TATGTAGCAG CTGTGGCAGA CCATATTCCC AAGGGAATCC TGCTGATGAG 
CCCCTGGAGA GAAGTGGGGT AGCCACTCGG ACACCAAGTA GAACAGATGA CACTGCTCAA 
GTTACCTCTG ATTATGAAAC CAATAACAAC AGTGACAGCA GTGACATTGT ACAGAATGAA 
GATGAAACAG AGTGCCTGAG AGAGCCTCTG AGGAAAGCAT CGGCTTGCAG CACCTATGCT 
CCTGAGACCA TGATGTTTCT GGACAAACCA ATTCTTGCTC CCGAACCTCT TGTCATGGAT 
AACCTGGACT CAATTATGGA GCAGCTAAAT ACTTGGAATT TTCCAATTTT TGATTTAGTG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTTAGTCAGG TATCTTACAG ACTTTTTGAA 
GACATGGGCC TCTTTGAAGC TTTTAAAATT CCAATTAGGG AATTTATGAA TTATTTTCAT 
GCTTTGGAGA TTGGATATAG GGATATTCCT TATCATAACA GAATCCATGC CACTGATGTT 
TTACATGCTG TTTGGTATCT TACTACACAG CCTATTCCAG GCCTCTCAAC TGTGATTAAT 
GATCATGGTT CAACCAGTGA TTCAGATTCT GACAGTGGAT TTACACATGG ACATATGGGA 
TATGTATTCT CAAAAACGTA TAATGTGACA GATGATAAAT ACGGATGTCT GTCTGGGAAT 
ATCCCTGCCT TGGAGTTGAT GGCGCTGTAT GTGGCTGCAG CCATGCACGA TTATGATCAT 
CCAGGAAGGA CTAATGCTTT CCTGGTTGCA ACTAGTGCTC CTCAGGCGGT GCTATATAAC 
GATCGTTCAG TTTTGGAGAA TCATCACGCA GCTGCTGCAT GGAATCTTTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAAT TAACCTTGAC CATGTGGAAT TTAAGCATTT CCGTTTCCTT 
GTCATTGAAG CAATTTTGGC CACTGACCTG AAGAAACACT TTGACTTCGT AGCCAAATTT 
AATGGCAAGG TAAATGATGA TGTTGGAATA GATTGGACCA ATGAAAATGA TCGTCTACTG 
GTTTGTCAAA TGTGTATAAA GTTGGCTGAT ATCAATGGTC CAGCTAAATG TAAAGAACTC 
CATCTTCAGT GGACAGATGG TATTGTCAAT GAATTTTATG AACAGGGTGA TGAAGAGGCC 
AGCCTTGGAT TACCCATAAG CCCCTTCATG GATCGTTCTG CTCCTCAGCT GGCCAACCTT 
CAGGAATCCT TCATCTCTCA CATTGTGGGG CCTCTGTGCA ACTCCTATGA TTCAGCAGGA 
CTAATGCCTG GAAAATGGGT GGAAGACAGC GATGAGTCAG GAGATACTGA TGACCCAGAA 
GAAGAGGAGG AAGAAGCACC AGCACCAAAT GAAGAGGAAA CCTGTGAAAA TAATGAATCT 
CCAAGCAAGT ACCAGATGCC TCTAGCCTAC CGTCTTGAAG ACACCCTCCA AGACAAGTTT 
CTTAAAGGAG GTATGCTAAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874909.1
CDS	22..3285
Translation	MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSLSFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLLCAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRLGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPSKYQMPLAYRLEDTLQDKFLKGGMLR

Target ORF information:

RefSeq Version	XM_017019420.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3A (PDE3A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019420.1

ORF Insert Sequence:

ATGGCAGTGC CCGGCGACGC TGCACGAGTC AGGGACAAGC CCGTCCACAG TGGGGTGAGT 
CAAGCCCCCA CGGCGGGCCG GGACTGCCAC CATCGTGCGG ACCCCGCATC GCCGCGGGAC 
TCGGGCTGCC GTGGCTGCTG GGGAGACCTG GTGCTGCAGC CGCTCCGGAG CTCTCGGAAA 
CTTTCCTCCG CGCTGTGCGC GGGCTCCCTG TCCTTTCTGC TGGCGCTGCT GGTGAGGCTG 
GTCCGCGGGG AGGTCGGCTG TGACCTGGAG CAGTGTAAGG AGGCGGCGGC GGCGGAGGAG 
GAGGAAGCAG CCCCGGGAGC AGAAGGGGGC GTCTTCCCGG GGCCTCGGGG AGGTGCTCCC 
GGGGGCGGTG CGCGGCTCAG CCCCTGGCTG CAGCCCTCGG CGCTGCTCTT CAGTCTCCTG 
TGTGCCTTCT TCTGGATGGG CTTGTACCTC CTGCGCGCCG GGGTGCGCCT GCCTCTGGCT 
GTCGCGCTGC TGGCCGCCTG CTGCGGGGGG GAAGCGCTCG TCCAGATTGG GCTGGGCGTC 
GGGGAGGATC ACTTACTCTC ACTCCCCGCC GCGGGGGTGG TGCTCAGCTG CTTGGCCGCC 
GCGACATGGC TGGTGCTGAG GCTGAGGCTG GGCGTCCTCA TGATCGCCTT GACTAGCGCG 
GTCAGGACCG TGTCCCTCAT TTCCTTAGAG AGGTTCAAGG TCGCCTGGAG ACCTTACCTG 
GCGTACCTGG CCGGCGTGCT GGGGATCCTC TTGGCCAGGT ACGTGGAACA AATCTTGCCG 
CAGTCCGCGG AGGCGGCTCC AAGGGAGCAT TTGGGGTCCC AGCTGATTGC TGGGACCAAG 
GAAGATATCC CGGTGTTTAA GAGGAGGAGG CGGTCCAGCT CCGTCGTGTC CGCCGAGATG 
TCCGGCTGCA GCAGCAAGTC CCATCGGAGG ACCTCCCTGC CCTGTATACC GAGGGAACAG 
CTCATGGGGC ATTCAGAATG GGACCACAAA CGAGGGCCAA GAGGATCACA GTCTTCAGGA 
ACCAGTATTA CTGTGGACAT CGCCGTCATG GGCGAGGCCC ACGGCCTCAT TACCGACCTC 
CTGGCAGACC CTTCTCTTCC ACCAAACGTG TGCACATCCT TGAGAGCCGT GAGCAACTTG 
CTCAGCACAC AGCTCACCTT CCAGGCCATT CACAAGCCCA GAGTGAATCC CGTCACTTCG 
CTCAGTGAAA ACTATACCTG TTCTGACTCT GAAGAGAGCT CTGAAAAAGA CAAGCTTGCT 
ATTCCAAAGC GCCTGAGAAG GAGTTTGCCT CCTGGCTTGT TGAGACGAGT TTCTTCCACT 
TGGACCACCA CCACCTCGGC CACAGGTCTA CCCACCTTGG AGCCTGCACC AGTACGGAGA 
GACCGCAGCA CCAGCATCAA ACTGCAGGAA GCACCTTCAT CCAGTCCTGA TTCTTGGAAT 
AATCCAGTGA TGATGACCCT CACCAAAAGC AGATCCTTTA CTTCATCCTA TGCTATTTCT 
GCAGCTAACC ATGTAAAGGC TAAAAAGCAA AGTCGACCAG GTGCCCTCGC TAAAATTTCA 
CCTCTTTCAT CGCCCTGCTC CTCACCTCTC CAAGGGACTC CTGCCAGCAG CCTGGTCAGC 
AAAATTTCTG CAGTGCAGTT TCCAGAATCT GCTGACACAA CTGCCAAACA AAGCCTAGGT 
TCTCACAGGG CCTTAACTTA CACTCAGAGT GCCCCAGACC TATCCCCTCA AATCCTGACT 
CCACCTGTTA TATGTAGCAG CTGTGGCAGA CCATATTCCC AAGGGAATCC TGCTGATGAG 
CCCCTGGAGA GAAGTGGGGT AGCCACTCGG ACACCAAGTA GAACAGATGA CACTGCTCAA 
GTTACCTCTG ATTATGAAAC CAATAACAAC AGTGACAGCA GTGACATTGT ACAGAATGAA 
GATGAAACAG AGTGCCTGAG AGAGCCTCTG AGGAAAGCAT CGGCTTGCAG CACCTATGCT 
CCTGAGACCA TGATGTTTCT GGACAAACCA ATTCTTGCTC CCGAACCTCT TGTCATGGAT 
AACCTGGACT CAATTATGGA GCAGCTAAAT ACTTGGAATT TTCCAATTTT TGATTTAGTG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTTAGTCAGG TATCTTACAG ACTTTTTGAA 
GACATGGGCC TCTTTGAAGC TTTTAAAATT CCAATTAGGG AATTTATGAA TTATTTTCAT 
GCTTTGGAGA TTGGATATAG GGATATTCCT TATCATAACA GAATCCATGC CACTGATGTT 
TTACATGCTG TTTGGTATCT TACTACACAG CCTATTCCAG GCCTCTCAAC TGTGATTAAT 
GATCATGGTT CAACCAGTGA TTCAGATTCT GACAGTGGAT TTACACATGG ACATATGGGA 
TATGTATTCT CAAAAACGTA TAATGTGACA GATGATAAAT ACGGATGTCT GTCTGGGAAT 
ATCCCTGCCT TGGAGTTGAT GGCGCTGTAT GTGGCTGCAG CCATGCACGA TTATGATCAT 
CCAGGAAGGA CTAATGCTTT CCTGGTTGCA ACTAGTGCTC CTCAGGCGGT GCTATATAAC 
GATCGTTCAG TTTTGGAGAA TCATCACGCA GCTGCTGCAT GGAATCTTTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAAT TAACCTTGAC CATGTGGAAT TTAAGCATTT CCGTTTCCTT 
GTCATTGAAG CAATTTTGGC CACTGACCTG AAGAAACACT TTGACTTCGT AGCCAAATTT 
AATGGCAAGG TAAATGATGA TGTTGGAATA GATTGGACCA ATGAAAATGA TCGTCTACTG 
GTTTGTCAAA TGTGTATAAA GTTGGCTGAT ATCAATGGTC CAGCTAAATG TAAAGAACTC 
CATCTTCAGT GGACAGATGG TATTGTCAAT GAATTTTATG AACAGGGTGA TGAAGAGGCC 
AGCCTTGGAT TACCCATAAG CCCCTTCATG GATCGTTCTG CTCCTCAGCT GGCCAACCTT 
CAGGAATCCT TCATCTCTCA CATTGTGGGG CCTCTGTGCA ACTCCTATGA TTCAGCAGGA 
CTAATGCCTG GAAAATGGGT GGAAGACAGC GATGAGTCAG GAGATACTGA TGACCCAGAA 
GAAGAGGAGG AAGAAGCACC AGCACCAAAT GAAGAGGAAA CCTGTGAAAA TAATGAATCT 
CCAAGCAAGT ACCAGATGCC TCTAGCCTAC CGTCTTGAAG ACACCCTCCA AGACAAGTTT 
CTTAAAGGAG GTATGCTAAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeting tumor cells based on Phosphodiesterase 3A expression.
Experimental cell research361(2)308-315(2017 12)
Nazir M,Senkowski W,Nyberg F,Blom K,Edqvist PH,Jarvius M,Andersson C,Gustafsson MG,Nygren P,Larsson R,Frykn?s M

PDE3A is hypermethylated in cisplatin resistant non-small cell lung cancer cells and is a modulator of chemotherapy response.
European review for medical and pharmacological sciences21(11)2635-2641(2017 Jun)
Tian FM,Zhong CY,Wang XN,Meng Y

cUMP hydrolysis by PDE3A.
Naunyn-Schmiedeberg's archives of pharmacology390(3)269-280(2017 Mar)
Berrisch S,Ostermeyer J,Kaever V,K?lble S,Hilfiker-Kleiner D,Seifert R,Schneider EH

Previously reported PDE3A-SLCO1C1 genetic variant does not correlate with anti-TNF response in a large UK rheumatoid arthritis cohort.
Pharmacogenomics17(7)715-20(2016 05)
Smith SL,Plant D,Lee XH,Massey J,Hyrich K,Morgan AW,Wilson AG,Isaacs J,Barton A

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PDE3A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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