DACT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DACT1
Entrez Gene ID	51339
Full Name	dishevelled binding antagonist of beta catenin 1
Synonyms	DAPPER,DAPPER1,DPR1,FRODO,HDPR1,TBS2,THYEX3
General protein information	Preferred Names dishevelled binding antagonist of beta catenin 1 Names dapper homolog 1 dapper antagonist of catenin 1 dapper, antagonist of beta-catenin, homolog 1 hepatocellular carcinoma novel gene 3 protein heptacellular carcinoma novel gene 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.1
Summary	The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	607861
Disorder Html:	?Townes-Brocks syndrome 2, 617466 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016651.5	NP_057735.2	dapper homolog 1 isoform 1
XM_006720167.3	XP_006720230.1	dapper homolog 1 isoform X1
NM_001079520.2	NP_001072988.1	dapper homolog 1 isoform 2
NM_016651.6	NP_057735.2	dapper homolog 1 isoform 1
NM_001079520.1	NP_001072988.1	dapper homolog 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dact1	NP_001177395.1
Danio rerio (zebrafish)	dact1	NP_999896.2
Homo sapiens (human)	DACT1	NP_057735.2
	DACT1	XP_001092593.1
Xenopus tropicalis (tropical clawed frog)	dact1	NP_001007950.1
Rattus norvegicus (Norway rat)	Dact1	XP_576044.1
Gallus gallus (chicken)	DACT1	NP_001038157.1
Pan troglodytes (chimpanzee)	DACT1	XP_001165895.1
Canis lupus familiaris (dog)	DACT1	XP_003435129.1
Bos taurus (cattle)	DACT1	XP_002691006.1

Gene Ontology
Bibliography

Related articles in PubMed

DACT1 overexpression inhibits proliferation, enhances apoptosis, and increases daunorubicin chemosensitivity in KG-1? cells.
Zhu K, Jiang B, Yang Y, Hu R, Liu Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(10)1010428317711089(2017 Oct)

DACT1 Overexpression in type I ovarian cancer inhibits malignant expansion and cis-platinum resistance by modulating canonical Wnt signalling and autophagy.
Li RN, Liu B, Li XM, Hou LS, Mu XL, Wang H, Linghu H
Scientific reports7(1)9285(2017 Aug)

Dapper1 attenuates hepatic gluconeogenesis and lipogenesis by activating PI3K/Akt signaling.
Kuang JR, Zhang ZH, Leng WL, Lei XT, Liang ZW
Molecular and cellular endocrinology447106-115(2017 May)

Aberrant methylation of DACT1 and DACT2 are associated with tumor progression and poor prognosis in esophageal squamous cell carcinoma.
Guo YL, Shan BE, Guo W, Dong ZM, Zhou Z, Shen SP, Guo X, Liang J, Kuang G
Journal of biomedical science24(1)6(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An inhibitory role for DACT1 in leukemogenesis.
Title: DACT1 overexpression inhibits proliferation, enhances apoptosis, and increases daunorubicin chemosensitivity in KG-1? cells.

Dact1 is up-regulated by TGF-beta1, inducing apoptosis in mesangial cells.
Title: Dact1, a Wnt-Pathway Inhibitor, Mediates Human Mesangial Cell TGF-?1-Induced Apoptosis.

Dapper1 attenuates hepatic gluconeogenesis and lipogenesis in Ttype 2 diabetes.
Title: Dapper1 attenuates hepatic gluconeogenesis and lipogenesis by activating PI3K/Akt signaling.

Findings suggest that the DACT1 c.1256G>A nonsense variant is causative of a specific genetic syndrome with features overlapping Townes-Brocks syndrome.
Title: Heterozygous Pathogenic Variant in DACT1 Causes an Autosomal-Dominant Syndrome with Features Overlapping Townes-Brocks Syndrome.

The simultaneous methylation of DACT1 and DACT2 may play important roles in progression of ESCC and may serve as prognostic methylation biomarkers for ESCC patients.
Title: Aberrant methylation of DACT1 and DACT2 are associated with tumor progression and poor prognosis in esophageal squamous cell carcinoma.

The following DACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18705	NM_016651.6 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu30261	NM_001079520.2 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$566.30 $809.00
OHu47738	XM_006720167.3 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu18705	NM_016651.5	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu30261	NM_001079520.1	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$566.30 $809.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACT1 ( NM_016651.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_057735.2 Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18705
Clone ID Related Accession (Same CDS sequence)	NM_016651.5 , NM_016651.6
Accession Version	NM_016651.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	dapper homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133312.3, CN304224.1, DR001289.1, BK000256.1 and BU742074.1. This sequence is a reference standard in the RefSeqGene project. On Jun 3, 2019 this sequence version replaced NM_016651.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000256.1, SRR1660807.33303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATTGTT GGAATATAAA 
GAAGGCCACT GTGAAGACCA GGCCTCAGGG GCAGTTTGCC GTTCCCTCTC CACACCACAA 
TTTAATTCCC TTGATGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGTGA TCTGGTGTCT 
AAAAACGGGA ATGATGTATA TCGCTATCCC AGTCCACTTC ATGCTGTGGC TGTGCAGAGC 
CCAATGTTTC TCCTTTGTCT GACGGGCAAC CCTCTGAGGG AAGAGGACAG GCTTGGAAAC 
CATGCCAGTG ACATTTGCGG TGGATCTGAG CTAGATGCCG TCAAAACAGA CAGTTCCTTA 
CCGTCCCCAA GCAGTCTGTG GTCTGCTTCC CATCCTTCAT CCAGCAAGAA AATGGATGGC 
TACATTCTGA GCCTGGTCCA GAAAAAAACA CACCCTGTAA GGACCAACAA ACCAAGAACC 
AGCGTGAACG CTGACCCCAC GAAAGGGCTT CTGAGGAACG GGAGCGTTTG TGTCAGAGCC 
CCGGGCGGTG TCTCACAGGG CAACAGTGTG AACCTTAAGA ATTCGAAACA GGCGTGTCTG 
CCCTCTGGCG GGATACCTTC TCTGAACAAT GGGACATTCT CCCCACCGAA GCAGTGGTCG 
AAAGAATCAA AGGCCGAACA AGCCGAAAGC AAGAGGGTGC CCCTGCCAGA GGGCTGCCCC 
TCAGGCGCTG CCTCCGACCT TCAGAGTAAG CACCTGCCAA AAACGGCCAA GCCAGCCTCG 
CAAGAACATG CTCGGTGTTC CGCCATTGGG ACAGGGGAGT CCCCTAAGGA AAGCGCTCAG 
CTCTCAGGGG CCTCTCCAAA AGAGAGTCCT AGCAGAGGCC CTGCCCCGCC GCAGGAGAAC 
AAAGTTGTAC AGCCCCTGAA AAAGATGTCA CAGAAAAACA GCCTGCAGGG CGTCCCCCCG 
GCCACTCCTC CCCTGCTGTC TACAGCTTTC CCCGTGGAAG AGAGGCCTGC CTTGGATTTC 
AAGAGCGAGG GCTCTTCCCA AAGCCTGGAG GAAGCGCACC TGGTCAAGGC CCAGTTTATC 
CCGGGGCAGC AGCCCAGTGT CAGGCTCCAC CGGGGCCACA GGAACATGGG CGTCGTGAAG 
AACTCCAGCC TGAAGCACCG CGGCCCAGCC CTCCAGGGGC TGGAGAACGG CTTGCCCACC 
GTCAGGGAGA AAACGCGGGC CGGGAGCAAG AAGTGTCGCT TCCCAGATGA CTTGGATACA 
AATAAGAAAC TCAAGAAAGC CTCCTCCAAG GGGAGGAAGA GTGGGGGCGG GCCCGAGGCT 
GGTGTTCCCG GCAGGCCCGC GGGCGGGGGC CACAGGGCGG GGAGCAGGGC GCATGGCCAC 
GGACGGGAGG CGGTGGTGGC CAAACCTAAG CACAAGCGAA CTGACTACCG GCGGTGGAAG 
TCCTCGGCCG AGATTTCCTA CGAAGAGGCC CTGAGGAGGG CCCGGCGCGG TCGCCGGGAG 
AATGTGGGGC TGTACCCCGC GCCTGTGCCT CTGCCCTACG CCAGCCCCTA CGCCTACGTG 
GCTAGCGACT CCGAGTACTC GGCCGAGTGC GAGTCCCTGT TCCACTCCAC CGTGGTGGAC 
ACCAGTGAGG ACGAGCAGAG CAATTACACC ACCAACTGCT TCGGGGACAG CGAGTCGAGT 
GTGAGCGAGG GCGAGTTCGT GGGGGAGAGC ACAACCACCA GCGACTCTGA AGAAAGCGGG 
GGCTTAATTT GGTCCCAGTT TGTCCAGACT CTGCCCATTC AAACGGTAAC GGCCCCAGAC 
CTTCACAACC ACCCCGCAAA AACCTTTGTC AAAATTAAGG CCTCACATAA CCTCAAGAAG 
AAGATCCTCC GCTTTCGGTC TGGCTCTTTG AAACTGATGA CGACGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057735.2
CDS	242..2752
Translation	MKPSPAGTAKELEPPAPARGEQRTAEPEGRWREKGEADTERQRTRERQEATLAGLAELEYLRQRQELLVRGALRGAGGAGAAAPRAGELLGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLSLSDGCPKSADLIGLLEYKEGHCEDQASGAVCRSLSTPQFNSLDVIADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	NM_016651.6
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016651.6

ORF Insert Sequence:

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATTGTT GGAATATAAA 
GAAGGCCACT GTGAAGACCA GGCCTCAGGG GCAGTTTGCC GTTCCCTCTC CACACCACAA 
TTTAATTCCC TTGATGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGTGA TCTGGTGTCT 
AAAAACGGGA ATGATGTATA TCGCTATCCC AGTCCACTTC ATGCTGTGGC TGTGCAGAGC 
CCAATGTTTC TCCTTTGTCT GACGGGCAAC CCTCTGAGGG AAGAGGACAG GCTTGGAAAC 
CATGCCAGTG ACATTTGCGG TGGATCTGAG CTAGATGCCG TCAAAACAGA CAGTTCCTTA 
CCGTCCCCAA GCAGTCTGTG GTCTGCTTCC CATCCTTCAT CCAGCAAGAA AATGGATGGC 
TACATTCTGA GCCTGGTCCA GAAAAAAACA CACCCTGTAA GGACCAACAA ACCAAGAACC 
AGCGTGAACG CTGACCCCAC GAAAGGGCTT CTGAGGAACG GGAGCGTTTG TGTCAGAGCC 
CCGGGCGGTG TCTCACAGGG CAACAGTGTG AACCTTAAGA ATTCGAAACA GGCGTGTCTG 
CCCTCTGGCG GGATACCTTC TCTGAACAAT GGGACATTCT CCCCACCGAA GCAGTGGTCG 
AAAGAATCAA AGGCCGAACA AGCCGAAAGC AAGAGGGTGC CCCTGCCAGA GGGCTGCCCC 
TCAGGCGCTG CCTCCGACCT TCAGAGTAAG CACCTGCCAA AAACGGCCAA GCCAGCCTCG 
CAAGAACATG CTCGGTGTTC CGCCATTGGG ACAGGGGAGT CCCCTAAGGA AAGCGCTCAG 
CTCTCAGGGG CCTCTCCAAA AGAGAGTCCT AGCAGAGGCC CTGCCCCGCC GCAGGAGAAC 
AAAGTTGTAC AGCCCCTGAA AAAGATGTCA CAGAAAAACA GCCTGCAGGG CGTCCCCCCG 
GCCACTCCTC CCCTGCTGTC TACAGCTTTC CCCGTGGAAG AGAGGCCTGC CTTGGATTTC 
AAGAGCGAGG GCTCTTCCCA AAGCCTGGAG GAAGCGCACC TGGTCAAGGC CCAGTTTATC 
CCGGGGCAGC AGCCCAGTGT CAGGCTCCAC CGGGGCCACA GGAACATGGG CGTCGTGAAG 
AACTCCAGCC TGAAGCACCG CGGCCCAGCC CTCCAGGGGC TGGAGAACGG CTTGCCCACC 
GTCAGGGAGA AAACGCGGGC CGGGAGCAAG AAGTGTCGCT TCCCAGATGA CTTGGATACA 
AATAAGAAAC TCAAGAAAGC CTCCTCCAAG GGGAGGAAGA GTGGGGGCGG GCCCGAGGCT 
GGTGTTCCCG GCAGGCCCGC GGGCGGGGGC CACAGGGCGG GGAGCAGGGC GCATGGCCAC 
GGACGGGAGG CGGTGGTGGC CAAACCTAAG CACAAGCGAA CTGACTACCG GCGGTGGAAG 
TCCTCGGCCG AGATTTCCTA CGAAGAGGCC CTGAGGAGGG CCCGGCGCGG TCGCCGGGAG 
AATGTGGGGC TGTACCCCGC GCCTGTGCCT CTGCCCTACG CCAGCCCCTA CGCCTACGTG 
GCTAGCGACT CCGAGTACTC GGCCGAGTGC GAGTCCCTGT TCCACTCCAC CGTGGTGGAC 
ACCAGTGAGG ACGAGCAGAG CAATTACACC ACCAACTGCT TCGGGGACAG CGAGTCGAGT 
GTGAGCGAGG GCGAGTTCGT GGGGGAGAGC ACAACCACCA GCGACTCTGA AGAAAGCGGG 
GGCTTAATTT GGTCCCAGTT TGTCCAGACT CTGCCCATTC AAACGGTAAC GGCCCCAGAC 
CTTCACAACC ACCCCGCAAA AACCTTTGTC AAAATTAAGG CCTCACATAA CCTCAAGAAG 
AAGATCCTCC GCTTTCGGTC TGGCTCTTTG AAACTGATGA CGACGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT1 ( NM_016651.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057735.2 Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18705
Clone ID Related Accession (Same CDS sequence)	NM_016651.5 , NM_016651.6
Accession Version	NM_016651.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	dapper homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN304224.1, DR001289.1, BK000256.1, AL133312.3 and BU742074.1. This sequence is a reference standard in the RefSeqGene project. On Nov 30, 2006 this sequence version replaced NM_016651.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000256.1, SRR1660807.33303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATTGTT GGAATATAAA 
GAAGGCCACT GTGAAGACCA GGCCTCAGGG GCAGTTTGCC GTTCCCTCTC CACACCACAA 
TTTAATTCCC TTGATGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGTGA TCTGGTGTCT 
AAAAACGGGA ATGATGTATA TCGCTATCCC AGTCCACTTC ATGCTGTGGC TGTGCAGAGC 
CCAATGTTTC TCCTTTGTCT GACGGGCAAC CCTCTGAGGG AAGAGGACAG GCTTGGAAAC 
CATGCCAGTG ACATTTGCGG TGGATCTGAG CTAGATGCCG TCAAAACAGA CAGTTCCTTA 
CCGTCCCCAA GCAGTCTGTG GTCTGCTTCC CATCCTTCAT CCAGCAAGAA AATGGATGGC 
TACATTCTGA GCCTGGTCCA GAAAAAAACA CACCCTGTAA GGACCAACAA ACCAAGAACC 
AGCGTGAACG CTGACCCCAC GAAAGGGCTT CTGAGGAACG GGAGCGTTTG TGTCAGAGCC 
CCGGGCGGTG TCTCACAGGG CAACAGTGTG AACCTTAAGA ATTCGAAACA GGCGTGTCTG 
CCCTCTGGCG GGATACCTTC TCTGAACAAT GGGACATTCT CCCCACCGAA GCAGTGGTCG 
AAAGAATCAA AGGCCGAACA AGCCGAAAGC AAGAGGGTGC CCCTGCCAGA GGGCTGCCCC 
TCAGGCGCTG CCTCCGACCT TCAGAGTAAG CACCTGCCAA AAACGGCCAA GCCAGCCTCG 
CAAGAACATG CTCGGTGTTC CGCCATTGGG ACAGGGGAGT CCCCTAAGGA AAGCGCTCAG 
CTCTCAGGGG CCTCTCCAAA AGAGAGTCCT AGCAGAGGCC CTGCCCCGCC GCAGGAGAAC 
AAAGTTGTAC AGCCCCTGAA AAAGATGTCA CAGAAAAACA GCCTGCAGGG CGTCCCCCCG 
GCCACTCCTC CCCTGCTGTC TACAGCTTTC CCCGTGGAAG AGAGGCCTGC CTTGGATTTC 
AAGAGCGAGG GCTCTTCCCA AAGCCTGGAG GAAGCGCACC TGGTCAAGGC CCAGTTTATC 
CCGGGGCAGC AGCCCAGTGT CAGGCTCCAC CGGGGCCACA GGAACATGGG CGTCGTGAAG 
AACTCCAGCC TGAAGCACCG CGGCCCAGCC CTCCAGGGGC TGGAGAACGG CTTGCCCACC 
GTCAGGGAGA AAACGCGGGC CGGGAGCAAG AAGTGTCGCT TCCCAGATGA CTTGGATACA 
AATAAGAAAC TCAAGAAAGC CTCCTCCAAG GGGAGGAAGA GTGGGGGCGG GCCCGAGGCT 
GGTGTTCCCG GCAGGCCCGC GGGCGGGGGC CACAGGGCGG GGAGCAGGGC GCATGGCCAC 
GGACGGGAGG CGGTGGTGGC CAAACCTAAG CACAAGCGAA CTGACTACCG GCGGTGGAAG 
TCCTCGGCCG AGATTTCCTA CGAAGAGGCC CTGAGGAGGG CCCGGCGCGG TCGCCGGGAG 
AATGTGGGGC TGTACCCCGC GCCTGTGCCT CTGCCCTACG CCAGCCCCTA CGCCTACGTG 
GCTAGCGACT CCGAGTACTC GGCCGAGTGC GAGTCCCTGT TCCACTCCAC CGTGGTGGAC 
ACCAGTGAGG ACGAGCAGAG CAATTACACC ACCAACTGCT TCGGGGACAG CGAGTCGAGT 
GTGAGCGAGG GCGAGTTCGT GGGGGAGAGC ACAACCACCA GCGACTCTGA AGAAAGCGGG 
GGCTTAATTT GGTCCCAGTT TGTCCAGACT CTGCCCATTC AAACGGTAAC GGCCCCAGAC 
CTTCACAACC ACCCCGCAAA AACCTTTGTC AAAATTAAGG CCTCACATAA CCTCAAGAAG 
AAGATCCTCC GCTTTCGGTC TGGCTCTTTG AAACTGATGA CGACGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057735.2
CDS	165..2675
Translation	MKPSPAGTAKELEPPAPARGEQRTAEPEGRWREKGEADTERQRTRERQEATLAGLAELEYLRQRQELLVRGALRGAGGAGAAAPRAGELLGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLSLSDGCPKSADLIGLLEYKEGHCEDQASGAVCRSLSTPQFNSLDVIADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	NM_016651.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016651.5

ORF Insert Sequence:

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATCTCA TAGGATTGTT GGAATATAAA 
GAAGGCCACT GTGAAGACCA GGCCTCAGGG GCAGTTTGCC GTTCCCTCTC CACACCACAA 
TTTAATTCCC TTGATGTCAT TGCAGATGTG AATCCCAAGT ACCAGTGTGA TCTGGTGTCT 
AAAAACGGGA ATGATGTATA TCGCTATCCC AGTCCACTTC ATGCTGTGGC TGTGCAGAGC 
CCAATGTTTC TCCTTTGTCT GACGGGCAAC CCTCTGAGGG AAGAGGACAG GCTTGGAAAC 
CATGCCAGTG ACATTTGCGG TGGATCTGAG CTAGATGCCG TCAAAACAGA CAGTTCCTTA 
CCGTCCCCAA GCAGTCTGTG GTCTGCTTCC CATCCTTCAT CCAGCAAGAA AATGGATGGC 
TACATTCTGA GCCTGGTCCA GAAAAAAACA CACCCTGTAA GGACCAACAA ACCAAGAACC 
AGCGTGAACG CTGACCCCAC GAAAGGGCTT CTGAGGAACG GGAGCGTTTG TGTCAGAGCC 
CCGGGCGGTG TCTCACAGGG CAACAGTGTG AACCTTAAGA ATTCGAAACA GGCGTGTCTG 
CCCTCTGGCG GGATACCTTC TCTGAACAAT GGGACATTCT CCCCACCGAA GCAGTGGTCG 
AAAGAATCAA AGGCCGAACA AGCCGAAAGC AAGAGGGTGC CCCTGCCAGA GGGCTGCCCC 
TCAGGCGCTG CCTCCGACCT TCAGAGTAAG CACCTGCCAA AAACGGCCAA GCCAGCCTCG 
CAAGAACATG CTCGGTGTTC CGCCATTGGG ACAGGGGAGT CCCCTAAGGA AAGCGCTCAG 
CTCTCAGGGG CCTCTCCAAA AGAGAGTCCT AGCAGAGGCC CTGCCCCGCC GCAGGAGAAC 
AAAGTTGTAC AGCCCCTGAA AAAGATGTCA CAGAAAAACA GCCTGCAGGG CGTCCCCCCG 
GCCACTCCTC CCCTGCTGTC TACAGCTTTC CCCGTGGAAG AGAGGCCTGC CTTGGATTTC 
AAGAGCGAGG GCTCTTCCCA AAGCCTGGAG GAAGCGCACC TGGTCAAGGC CCAGTTTATC 
CCGGGGCAGC AGCCCAGTGT CAGGCTCCAC CGGGGCCACA GGAACATGGG CGTCGTGAAG 
AACTCCAGCC TGAAGCACCG CGGCCCAGCC CTCCAGGGGC TGGAGAACGG CTTGCCCACC 
GTCAGGGAGA AAACGCGGGC CGGGAGCAAG AAGTGTCGCT TCCCAGATGA CTTGGATACA 
AATAAGAAAC TCAAGAAAGC CTCCTCCAAG GGGAGGAAGA GTGGGGGCGG GCCCGAGGCT 
GGTGTTCCCG GCAGGCCCGC GGGCGGGGGC CACAGGGCGG GGAGCAGGGC GCATGGCCAC 
GGACGGGAGG CGGTGGTGGC CAAACCTAAG CACAAGCGAA CTGACTACCG GCGGTGGAAG 
TCCTCGGCCG AGATTTCCTA CGAAGAGGCC CTGAGGAGGG CCCGGCGCGG TCGCCGGGAG 
AATGTGGGGC TGTACCCCGC GCCTGTGCCT CTGCCCTACG CCAGCCCCTA CGCCTACGTG 
GCTAGCGACT CCGAGTACTC GGCCGAGTGC GAGTCCCTGT TCCACTCCAC CGTGGTGGAC 
ACCAGTGAGG ACGAGCAGAG CAATTACACC ACCAACTGCT TCGGGGACAG CGAGTCGAGT 
GTGAGCGAGG GCGAGTTCGT GGGGGAGAGC ACAACCACCA GCGACTCTGA AGAAAGCGGG 
GGCTTAATTT GGTCCCAGTT TGTCCAGACT CTGCCCATTC AAACGGTAAC GGCCCCAGAC 
CTTCACAACC ACCCCGCAAA AACCTTTGTC AAAATTAAGG CCTCACATAA CCTCAAGAAG 
AAGATCCTCC GCTTTCGGTC TGGCTCTTTG AAACTGATGA CGACGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT1 ( NM_001079520.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001072988.1 Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30261
Clone ID Related Accession (Same CDS sequence)	NM_001079520.2 , NM_001079520.1
Accession Version	NM_001079520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	dapper homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133312.3, CN304224.1, DR001289.1, DR157190.1 and BU742074.1. On Jun 2, 2019 this sequence version replaced NM_001079520.1. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.444163.1, SRR1803612.482533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGTGAT 
CTGGTGTCTA AAAACGGGAA TGATGTATAT CGCTATCCCA GTCCACTTCA TGCTGTGGCT 
GTGCAGAGCC CAATGTTTCT CCTTTGTCTG ACGGGCAACC CTCTGAGGGA AGAGGACAGG 
CTTGGAAACC ATGCCAGTGA CATTTGCGGT GGATCTGAGC TAGATGCCGT CAAAACAGAC 
AGTTCCTTAC CGTCCCCAAG CAGTCTGTGG TCTGCTTCCC ATCCTTCATC CAGCAAGAAA 
ATGGATGGCT ACATTCTGAG CCTGGTCCAG AAAAAAACAC ACCCTGTAAG GACCAACAAA 
CCAAGAACCA GCGTGAACGC TGACCCCACG AAAGGGCTTC TGAGGAACGG GAGCGTTTGT 
GTCAGAGCCC CGGGCGGTGT CTCACAGGGC AACAGTGTGA ACCTTAAGAA TTCGAAACAG 
GCGTGTCTGC CCTCTGGCGG GATACCTTCT CTGAACAATG GGACATTCTC CCCACCGAAG 
CAGTGGTCGA AAGAATCAAA GGCCGAACAA GCCGAAAGCA AGAGGGTGCC CCTGCCAGAG 
GGCTGCCCCT CAGGCGCTGC CTCCGACCTT CAGAGTAAGC ACCTGCCAAA AACGGCCAAG 
CCAGCCTCGC AAGAACATGC TCGGTGTTCC GCCATTGGGA CAGGGGAGTC CCCTAAGGAA 
AGCGCTCAGC TCTCAGGGGC CTCTCCAAAA GAGAGTCCTA GCAGAGGCCC TGCCCCGCCG 
CAGGAGAACA AAGTTGTACA GCCCCTGAAA AAGATGTCAC AGAAAAACAG CCTGCAGGGC 
GTCCCCCCGG CCACTCCTCC CCTGCTGTCT ACAGCTTTCC CCGTGGAAGA GAGGCCTGCC 
TTGGATTTCA AGAGCGAGGG CTCTTCCCAA AGCCTGGAGG AAGCGCACCT GGTCAAGGCC 
CAGTTTATCC CGGGGCAGCA GCCCAGTGTC AGGCTCCACC GGGGCCACAG GAACATGGGC 
GTCGTGAAGA ACTCCAGCCT GAAGCACCGC GGCCCAGCCC TCCAGGGGCT GGAGAACGGC 
TTGCCCACCG TCAGGGAGAA AACGCGGGCC GGGAGCAAGA AGTGTCGCTT CCCAGATGAC 
TTGGATACAA ATAAGAAACT CAAGAAAGCC TCCTCCAAGG GGAGGAAGAG TGGGGGCGGG 
CCCGAGGCTG GTGTTCCCGG CAGGCCCGCG GGCGGGGGCC ACAGGGCGGG GAGCAGGGCG 
CATGGCCACG GACGGGAGGC GGTGGTGGCC AAACCTAAGC ACAAGCGAAC TGACTACCGG 
CGGTGGAAGT CCTCGGCCGA GATTTCCTAC GAAGAGGCCC TGAGGAGGGC CCGGCGCGGT 
CGCCGGGAGA ATGTGGGGCT GTACCCCGCG CCTGTGCCTC TGCCCTACGC CAGCCCCTAC 
GCCTACGTGG CTAGCGACTC CGAGTACTCG GCCGAGTGCG AGTCCCTGTT CCACTCCACC 
GTGGTGGACA CCAGTGAGGA CGAGCAGAGC AATTACACCA CCAACTGCTT CGGGGACAGC 
GAGTCGAGTG TGAGCGAGGG CGAGTTCGTG GGGGAGAGCA CAACCACCAG CGACTCTGAA 
GAAAGCGGGG GCTTAATTTG GTCCCAGTTT GTCCAGACTC TGCCCATTCA AACGGTAACG 
GCCCCAGACC TTCACAACCA CCCCGCAAAA ACCTTTGTCA AAATTAAGGC CTCACATAAC 
CTCAAGAAGA AGATCCTCCG CTTTCGGTCT GGCTCTTTGA AACTGATGAC GACGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072988.1
CDS	242..2641
Translation	MKPSPAGTAKELEPPAPARGEQRTAEPEGRWREKGEADTERQRTRERQEATLAGLAELEYLRQRQELLVRGALRGAGGAGAAAPRAGELLGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLSLSDGCPKSADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	NM_001079520.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079520.2

ORF Insert Sequence:

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGTGAT 
CTGGTGTCTA AAAACGGGAA TGATGTATAT CGCTATCCCA GTCCACTTCA TGCTGTGGCT 
GTGCAGAGCC CAATGTTTCT CCTTTGTCTG ACGGGCAACC CTCTGAGGGA AGAGGACAGG 
CTTGGAAACC ATGCCAGTGA CATTTGCGGT GGATCTGAGC TAGATGCCGT CAAAACAGAC 
AGTTCCTTAC CGTCCCCAAG CAGTCTGTGG TCTGCTTCCC ATCCTTCATC CAGCAAGAAA 
ATGGATGGCT ACATTCTGAG CCTGGTCCAG AAAAAAACAC ACCCTGTAAG GACCAACAAA 
CCAAGAACCA GCGTGAACGC TGACCCCACG AAAGGGCTTC TGAGGAACGG GAGCGTTTGT 
GTCAGAGCCC CGGGCGGTGT CTCACAGGGC AACAGTGTGA ACCTTAAGAA TTCGAAACAG 
GCGTGTCTGC CCTCTGGCGG GATACCTTCT CTGAACAATG GGACATTCTC CCCACCGAAG 
CAGTGGTCGA AAGAATCAAA GGCCGAACAA GCCGAAAGCA AGAGGGTGCC CCTGCCAGAG 
GGCTGCCCCT CAGGCGCTGC CTCCGACCTT CAGAGTAAGC ACCTGCCAAA AACGGCCAAG 
CCAGCCTCGC AAGAACATGC TCGGTGTTCC GCCATTGGGA CAGGGGAGTC CCCTAAGGAA 
AGCGCTCAGC TCTCAGGGGC CTCTCCAAAA GAGAGTCCTA GCAGAGGCCC TGCCCCGCCG 
CAGGAGAACA AAGTTGTACA GCCCCTGAAA AAGATGTCAC AGAAAAACAG CCTGCAGGGC 
GTCCCCCCGG CCACTCCTCC CCTGCTGTCT ACAGCTTTCC CCGTGGAAGA GAGGCCTGCC 
TTGGATTTCA AGAGCGAGGG CTCTTCCCAA AGCCTGGAGG AAGCGCACCT GGTCAAGGCC 
CAGTTTATCC CGGGGCAGCA GCCCAGTGTC AGGCTCCACC GGGGCCACAG GAACATGGGC 
GTCGTGAAGA ACTCCAGCCT GAAGCACCGC GGCCCAGCCC TCCAGGGGCT GGAGAACGGC 
TTGCCCACCG TCAGGGAGAA AACGCGGGCC GGGAGCAAGA AGTGTCGCTT CCCAGATGAC 
TTGGATACAA ATAAGAAACT CAAGAAAGCC TCCTCCAAGG GGAGGAAGAG TGGGGGCGGG 
CCCGAGGCTG GTGTTCCCGG CAGGCCCGCG GGCGGGGGCC ACAGGGCGGG GAGCAGGGCG 
CATGGCCACG GACGGGAGGC GGTGGTGGCC AAACCTAAGC ACAAGCGAAC TGACTACCGG 
CGGTGGAAGT CCTCGGCCGA GATTTCCTAC GAAGAGGCCC TGAGGAGGGC CCGGCGCGGT 
CGCCGGGAGA ATGTGGGGCT GTACCCCGCG CCTGTGCCTC TGCCCTACGC CAGCCCCTAC 
GCCTACGTGG CTAGCGACTC CGAGTACTCG GCCGAGTGCG AGTCCCTGTT CCACTCCACC 
GTGGTGGACA CCAGTGAGGA CGAGCAGAGC AATTACACCA CCAACTGCTT CGGGGACAGC 
GAGTCGAGTG TGAGCGAGGG CGAGTTCGTG GGGGAGAGCA CAACCACCAG CGACTCTGAA 
GAAAGCGGGG GCTTAATTTG GTCCCAGTTT GTCCAGACTC TGCCCATTCA AACGGTAACG 
GCCCCAGACC TTCACAACCA CCCCGCAAAA ACCTTTGTCA AAATTAAGGC CTCACATAAC 
CTCAAGAAGA AGATCCTCCG CTTTCGGTCT GGCTCTTTGA AACTGATGAC GACGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT1 ( NM_001079520.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001072988.1 Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30261
Clone ID Related Accession (Same CDS sequence)	NM_001079520.2 , NM_001079520.1
Accession Version	NM_001079520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	dapper homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN304224.1, DR001289.1, DR157190.1, AL133312.3 and BU742074.1. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.444163.1, ERR279840.6121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGTGAT 
CTGGTGTCTA AAAACGGGAA TGATGTATAT CGCTATCCCA GTCCACTTCA TGCTGTGGCT 
GTGCAGAGCC CAATGTTTCT CCTTTGTCTG ACGGGCAACC CTCTGAGGGA AGAGGACAGG 
CTTGGAAACC ATGCCAGTGA CATTTGCGGT GGATCTGAGC TAGATGCCGT CAAAACAGAC 
AGTTCCTTAC CGTCCCCAAG CAGTCTGTGG TCTGCTTCCC ATCCTTCATC CAGCAAGAAA 
ATGGATGGCT ACATTCTGAG CCTGGTCCAG AAAAAAACAC ACCCTGTAAG GACCAACAAA 
CCAAGAACCA GCGTGAACGC TGACCCCACG AAAGGGCTTC TGAGGAACGG GAGCGTTTGT 
GTCAGAGCCC CGGGCGGTGT CTCACAGGGC AACAGTGTGA ACCTTAAGAA TTCGAAACAG 
GCGTGTCTGC CCTCTGGCGG GATACCTTCT CTGAACAATG GGACATTCTC CCCACCGAAG 
CAGTGGTCGA AAGAATCAAA GGCCGAACAA GCCGAAAGCA AGAGGGTGCC CCTGCCAGAG 
GGCTGCCCCT CAGGCGCTGC CTCCGACCTT CAGAGTAAGC ACCTGCCAAA AACGGCCAAG 
CCAGCCTCGC AAGAACATGC TCGGTGTTCC GCCATTGGGA CAGGGGAGTC CCCTAAGGAA 
AGCGCTCAGC TCTCAGGGGC CTCTCCAAAA GAGAGTCCTA GCAGAGGCCC TGCCCCGCCG 
CAGGAGAACA AAGTTGTACA GCCCCTGAAA AAGATGTCAC AGAAAAACAG CCTGCAGGGC 
GTCCCCCCGG CCACTCCTCC CCTGCTGTCT ACAGCTTTCC CCGTGGAAGA GAGGCCTGCC 
TTGGATTTCA AGAGCGAGGG CTCTTCCCAA AGCCTGGAGG AAGCGCACCT GGTCAAGGCC 
CAGTTTATCC CGGGGCAGCA GCCCAGTGTC AGGCTCCACC GGGGCCACAG GAACATGGGC 
GTCGTGAAGA ACTCCAGCCT GAAGCACCGC GGCCCAGCCC TCCAGGGGCT GGAGAACGGC 
TTGCCCACCG TCAGGGAGAA AACGCGGGCC GGGAGCAAGA AGTGTCGCTT CCCAGATGAC 
TTGGATACAA ATAAGAAACT CAAGAAAGCC TCCTCCAAGG GGAGGAAGAG TGGGGGCGGG 
CCCGAGGCTG GTGTTCCCGG CAGGCCCGCG GGCGGGGGCC ACAGGGCGGG GAGCAGGGCG 
CATGGCCACG GACGGGAGGC GGTGGTGGCC AAACCTAAGC ACAAGCGAAC TGACTACCGG 
CGGTGGAAGT CCTCGGCCGA GATTTCCTAC GAAGAGGCCC TGAGGAGGGC CCGGCGCGGT 
CGCCGGGAGA ATGTGGGGCT GTACCCCGCG CCTGTGCCTC TGCCCTACGC CAGCCCCTAC 
GCCTACGTGG CTAGCGACTC CGAGTACTCG GCCGAGTGCG AGTCCCTGTT CCACTCCACC 
GTGGTGGACA CCAGTGAGGA CGAGCAGAGC AATTACACCA CCAACTGCTT CGGGGACAGC 
GAGTCGAGTG TGAGCGAGGG CGAGTTCGTG GGGGAGAGCA CAACCACCAG CGACTCTGAA 
GAAAGCGGGG GCTTAATTTG GTCCCAGTTT GTCCAGACTC TGCCCATTCA AACGGTAACG 
GCCCCAGACC TTCACAACCA CCCCGCAAAA ACCTTTGTCA AAATTAAGGC CTCACATAAC 
CTCAAGAAGA AGATCCTCCG CTTTCGGTCT GGCTCTTTGA AACTGATGAC GACGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072988.1
CDS	165..2564
Translation	MKPSPAGTAKELEPPAPARGEQRTAEPEGRWREKGEADTERQRTRERQEATLAGLAELEYLRQRQELLVRGALRGAGGAGAAAPRAGELLGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLSLSDGCPKSADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	NM_001079520.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079520.1

ORF Insert Sequence:

ATGAAGCCGA GTCCGGCCGG GACGGCGAAG GAGCTGGAGC CTCCGGCGCC GGCCCGAGGC 
GAGCAGCGCA CGGCGGAGCC CGAGGGGCGC TGGCGGGAGA AGGGCGAGGC AGACACCGAG 
CGGCAGCGCA CCCGGGAGCG GCAGGAGGCC ACGCTGGCCG GGCTGGCGGA GCTGGAGTAC 
CTGCGCCAGC GCCAAGAGCT GCTGGTCAGG GGCGCCCTGC GCGGCGCCGG GGGTGCGGGA 
GCCGCTGCGC CCCGCGCTGG GGAGCTACTG GGGGAGGCGG CGCAGCGCAG TCGCCTGGAG 
GAGAAGTTCT TGGAGGAGAA CATCTTGCTG CTAAGAAAGC AATTGAACTG TTTGAGGCGA 
AGAGATGCTG GTTTGTTGAA TCAGTTGCAA GAGCTTGACA AGCAGATAAG TGACCTGAGA 
CTGGATGTAG AAAAGACATC TGAAGAGCAC CTGGAGACAG ACAGTCGGCC TAGCTCAGGG 
TTTTATGAGC TGAGTGATGG GGCTTCAGGA TCCCTTTCCA ATTCCTCTAA CTCGGTGTTC 
AGTGAGTGTT TATCCAGTTG TCATTCCAGC ACCTGCTTTT GCAGCCCCTT GGAGGCGACC 
TTGAGTCTCT CAGATGGTTG CCCCAAATCT GCAGATGTGA ATCCCAAGTA CCAGTGTGAT 
CTGGTGTCTA AAAACGGGAA TGATGTATAT CGCTATCCCA GTCCACTTCA TGCTGTGGCT 
GTGCAGAGCC CAATGTTTCT CCTTTGTCTG ACGGGCAACC CTCTGAGGGA AGAGGACAGG 
CTTGGAAACC ATGCCAGTGA CATTTGCGGT GGATCTGAGC TAGATGCCGT CAAAACAGAC 
AGTTCCTTAC CGTCCCCAAG CAGTCTGTGG TCTGCTTCCC ATCCTTCATC CAGCAAGAAA 
ATGGATGGCT ACATTCTGAG CCTGGTCCAG AAAAAAACAC ACCCTGTAAG GACCAACAAA 
CCAAGAACCA GCGTGAACGC TGACCCCACG AAAGGGCTTC TGAGGAACGG GAGCGTTTGT 
GTCAGAGCCC CGGGCGGTGT CTCACAGGGC AACAGTGTGA ACCTTAAGAA TTCGAAACAG 
GCGTGTCTGC CCTCTGGCGG GATACCTTCT CTGAACAATG GGACATTCTC CCCACCGAAG 
CAGTGGTCGA AAGAATCAAA GGCCGAACAA GCCGAAAGCA AGAGGGTGCC CCTGCCAGAG 
GGCTGCCCCT CAGGCGCTGC CTCCGACCTT CAGAGTAAGC ACCTGCCAAA AACGGCCAAG 
CCAGCCTCGC AAGAACATGC TCGGTGTTCC GCCATTGGGA CAGGGGAGTC CCCTAAGGAA 
AGCGCTCAGC TCTCAGGGGC CTCTCCAAAA GAGAGTCCTA GCAGAGGCCC TGCCCCGCCG 
CAGGAGAACA AAGTTGTACA GCCCCTGAAA AAGATGTCAC AGAAAAACAG CCTGCAGGGC 
GTCCCCCCGG CCACTCCTCC CCTGCTGTCT ACAGCTTTCC CCGTGGAAGA GAGGCCTGCC 
TTGGATTTCA AGAGCGAGGG CTCTTCCCAA AGCCTGGAGG AAGCGCACCT GGTCAAGGCC 
CAGTTTATCC CGGGGCAGCA GCCCAGTGTC AGGCTCCACC GGGGCCACAG GAACATGGGC 
GTCGTGAAGA ACTCCAGCCT GAAGCACCGC GGCCCAGCCC TCCAGGGGCT GGAGAACGGC 
TTGCCCACCG TCAGGGAGAA AACGCGGGCC GGGAGCAAGA AGTGTCGCTT CCCAGATGAC 
TTGGATACAA ATAAGAAACT CAAGAAAGCC TCCTCCAAGG GGAGGAAGAG TGGGGGCGGG 
CCCGAGGCTG GTGTTCCCGG CAGGCCCGCG GGCGGGGGCC ACAGGGCGGG GAGCAGGGCG 
CATGGCCACG GACGGGAGGC GGTGGTGGCC AAACCTAAGC ACAAGCGAAC TGACTACCGG 
CGGTGGAAGT CCTCGGCCGA GATTTCCTAC GAAGAGGCCC TGAGGAGGGC CCGGCGCGGT 
CGCCGGGAGA ATGTGGGGCT GTACCCCGCG CCTGTGCCTC TGCCCTACGC CAGCCCCTAC 
GCCTACGTGG CTAGCGACTC CGAGTACTCG GCCGAGTGCG AGTCCCTGTT CCACTCCACC 
GTGGTGGACA CCAGTGAGGA CGAGCAGAGC AATTACACCA CCAACTGCTT CGGGGACAGC 
GAGTCGAGTG TGAGCGAGGG CGAGTTCGTG GGGGAGAGCA CAACCACCAG CGACTCTGAA 
GAAAGCGGGG GCTTAATTTG GTCCCAGTTT GTCCAGACTC TGCCCATTCA AACGGTAACG 
GCCCCAGACC TTCACAACCA CCCCGCAAAA ACCTTTGTCA AAATTAAGGC CTCACATAAC 
CTCAAGAAGA AGATCCTCCG CTTTCGGTCT GGCTCTTTGA AACTGATGAC GACGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT1 ( XM_006720167.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720230.1 Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X1, mRNA.

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CloneID	OHu47738
Clone ID Related Accession (Same CDS sequence)	XM_006720167.3
Accession Version	XM_006720167.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dapper homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCC TTTGTCTGAC GGGCAACCCT CTGAGGGAAG AGGACAGGCT TGGAAACCAT 
GCCAGTGACA TTTGCGGTGG ATCTGAGCTA GATGCCGTCA AAACAGACAG TTCCTTACCG 
TCCCCAAGCA GTCTGTGGTC TGCTTCCCAT CCTTCATCCA GCAAGAAAAT GGATGGCTAC 
ATTCTGAGCC TGGTCCAGAA AAAAACACAC CCTGTAAGGA CCAACAAACC AAGAACCAGC 
GTGAACGCTG ACCCCACGAA AGGGCTTCTG AGGAACGGGA GCGTTTGTGT CAGAGCCCCG 
GGCGGTGTCT CACAGGGCAA CAGTGTGAAC CTTAAGAATT CGAAACAGGC GTGTCTGCCC 
TCTGGCGGGA TACCTTCTCT GAACAATGGG ACATTCTCCC CACCGAAGCA GTGGTCGAAA 
GAATCAAAGG CCGAACAAGC CGAAAGCAAG AGGGTGCCCC TGCCAGAGGG CTGCCCCTCA 
GGCGCTGCCT CCGACCTTCA GAGTAAGCAC CTGCCAAAAA CGGCCAAGCC AGCCTCGCAA 
GAACATGCTC GGTGTTCCGC CATTGGGACA GGGGAGTCCC CTAAGGAAAG CGCTCAGCTC 
TCAGGGGCCT CTCCAAAAGA GAGTCCTAGC AGAGGCCCTG CCCCGCCGCA GGAGAACAAA 
GTTGTACAGC CCCTGAAAAA GATGTCACAG AAAAACAGCC TGCAGGGCGT CCCCCCGGCC 
ACTCCTCCCC TGCTGTCTAC AGCTTTCCCC GTGGAAGAGA GGCCTGCCTT GGATTTCAAG 
AGCGAGGGCT CTTCCCAAAG CCTGGAGGAA GCGCACCTGG TCAAGGCCCA GTTTATCCCG 
GGGCAGCAGC CCAGTGTCAG GCTCCACCGG GGCCACAGGA ACATGGGCGT CGTGAAGAAC 
TCCAGCCTGA AGCACCGCGG CCCAGCCCTC CAGGGGCTGG AGAACGGCTT GCCCACCGTC 
AGGGAGAAAA CGCGGGCCGG GAGCAAGAAG TGTCGCTTCC CAGATGACTT GGATACAAAT 
AAGAAACTCA AGAAAGCCTC CTCCAAGGGG AGGAAGAGTG GGGGCGGGCC CGAGGCTGGT 
GTTCCCGGCA GGCCCGCGGG CGGGGGCCAC AGGGCGGGGA GCAGGGCGCA TGGCCACGGA 
CGGGAGGCGG TGGTGGCCAA ACCTAAGCAC AAGCGAACTG ACTACCGGCG GTGGAAGTCC 
TCGGCCGAGA TTTCCTACGA AGAGGCCCTG AGGAGGGCCC GGCGCGGTCG CCGGGAGAAT 
GTGGGGCTGT ACCCCGCGCC TGTGCCTCTG CCCTACGCCA GCCCCTACGC CTACGTGGCT 
AGCGACTCCG AGTACTCGGC CGAGTGCGAG TCCCTGTTCC ACTCCACCGT GGTGGACACC 
AGTGAGGACG AGCAGAGCAA TTACACCACC AACTGCTTCG GGGACAGCGA GTCGAGTGTG 
AGCGAGGGCG AGTTCGTGGG GGAGAGCACA ACCACCAGCG ACTCTGAAGA AAGCGGGGGC 
TTAATTTGGT CCCAGTTTGT CCAGACTCTG CCCATTCAAA CGGTAACGGC CCCAGACCTT 
CACAACCACC CCGCAAAAAC CTTTGTCAAA ATTAAGGCCT CACATAACCT CAAGAAGAAG 
ATCCTCCGCT TTCGGTCTGG CTCTTTGAAA CTGATGACGA CGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720230.1
CDS	945..2612
Translation	MFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	XM_006720167.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 1 (DACT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720167.3

ORF Insert Sequence:

ATGTTTCTCC TTTGTCTGAC GGGCAACCCT CTGAGGGAAG AGGACAGGCT TGGAAACCAT 
GCCAGTGACA TTTGCGGTGG ATCTGAGCTA GATGCCGTCA AAACAGACAG TTCCTTACCG 
TCCCCAAGCA GTCTGTGGTC TGCTTCCCAT CCTTCATCCA GCAAGAAAAT GGATGGCTAC 
ATTCTGAGCC TGGTCCAGAA AAAAACACAC CCTGTAAGGA CCAACAAACC AAGAACCAGC 
GTGAACGCTG ACCCCACGAA AGGGCTTCTG AGGAACGGGA GCGTTTGTGT CAGAGCCCCG 
GGCGGTGTCT CACAGGGCAA CAGTGTGAAC CTTAAGAATT CGAAACAGGC GTGTCTGCCC 
TCTGGCGGGA TACCTTCTCT GAACAATGGG ACATTCTCCC CACCGAAGCA GTGGTCGAAA 
GAATCAAAGG CCGAACAAGC CGAAAGCAAG AGGGTGCCCC TGCCAGAGGG CTGCCCCTCA 
GGCGCTGCCT CCGACCTTCA GAGTAAGCAC CTGCCAAAAA CGGCCAAGCC AGCCTCGCAA 
GAACATGCTC GGTGTTCCGC CATTGGGACA GGGGAGTCCC CTAAGGAAAG CGCTCAGCTC 
TCAGGGGCCT CTCCAAAAGA GAGTCCTAGC AGAGGCCCTG CCCCGCCGCA GGAGAACAAA 
GTTGTACAGC CCCTGAAAAA GATGTCACAG AAAAACAGCC TGCAGGGCGT CCCCCCGGCC 
ACTCCTCCCC TGCTGTCTAC AGCTTTCCCC GTGGAAGAGA GGCCTGCCTT GGATTTCAAG 
AGCGAGGGCT CTTCCCAAAG CCTGGAGGAA GCGCACCTGG TCAAGGCCCA GTTTATCCCG 
GGGCAGCAGC CCAGTGTCAG GCTCCACCGG GGCCACAGGA ACATGGGCGT CGTGAAGAAC 
TCCAGCCTGA AGCACCGCGG CCCAGCCCTC CAGGGGCTGG AGAACGGCTT GCCCACCGTC 
AGGGAGAAAA CGCGGGCCGG GAGCAAGAAG TGTCGCTTCC CAGATGACTT GGATACAAAT 
AAGAAACTCA AGAAAGCCTC CTCCAAGGGG AGGAAGAGTG GGGGCGGGCC CGAGGCTGGT 
GTTCCCGGCA GGCCCGCGGG CGGGGGCCAC AGGGCGGGGA GCAGGGCGCA TGGCCACGGA 
CGGGAGGCGG TGGTGGCCAA ACCTAAGCAC AAGCGAACTG ACTACCGGCG GTGGAAGTCC 
TCGGCCGAGA TTTCCTACGA AGAGGCCCTG AGGAGGGCCC GGCGCGGTCG CCGGGAGAAT 
GTGGGGCTGT ACCCCGCGCC TGTGCCTCTG CCCTACGCCA GCCCCTACGC CTACGTGGCT 
AGCGACTCCG AGTACTCGGC CGAGTGCGAG TCCCTGTTCC ACTCCACCGT GGTGGACACC 
AGTGAGGACG AGCAGAGCAA TTACACCACC AACTGCTTCG GGGACAGCGA GTCGAGTGTG 
AGCGAGGGCG AGTTCGTGGG GGAGAGCACA ACCACCAGCG ACTCTGAAGA AAGCGGGGGC 
TTAATTTGGT CCCAGTTTGT CCAGACTCTG CCCATTCAAA CGGTAACGGC CCCAGACCTT 
CACAACCACC CCGCAAAAAC CTTTGTCAAA ATTAAGGCCT CACATAACCT CAAGAAGAAG 
ATCCTCCGCT TTCGGTCTGG CTCTTTGAAA CTGATGACGA CGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DACT1 overexpression inhibits proliferation, enhances apoptosis, and increases daunorubicin chemosensitivity in KG-1? cells.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(10)1010428317711089(2017 Oct)
Zhu K,Jiang B,Yang Y,Hu R,Liu Z

DACT1 Overexpression in type I ovarian cancer inhibits malignant expansion and cis-platinum resistance by modulating canonical Wnt signalling and autophagy.
Scientific reports7(1)9285(2017 Aug)
Li RN,Liu B,Li XM,Hou LS,Mu XL,Wang H,Linghu H

Dapper1 attenuates hepatic gluconeogenesis and lipogenesis by activating PI3K/Akt signaling.
Molecular and cellular endocrinology447106-115(2017 May)
Kuang JR,Zhang ZH,Leng WL,Lei XT,Liang ZW

Aberrant methylation of DACT1 and DACT2 are associated with tumor progression and poor prognosis in esophageal squamous cell carcinoma.
Journal of biomedical science24(1)6(2017 Jan)
Guo YL,Shan BE,Guo W,Dong ZM,Zhou Z,Shen SP,Guo X,Liang J,Kuang G

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DACT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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