gRNAs
The following SPTBN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu15258 | |
| Clone ID Related Accession (Same CDS sequence) | NM_016642.4 , NM_016642.3 | |
| Accession Version | NM_016642.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11025bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-02 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF233523.1, AC020659.5 and AA599654.1. On Nov 22, 2018 this sequence version replaced NM_016642.3. ##Evidence-Data-START## Transcript exon combination :: AF233523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000320955.8/ ENSP00000317790.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_057726.4 |
| CDS | 205..11229 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_016642.4 |
1 | ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu15258 | |
| Clone ID Related Accession (Same CDS sequence) | NM_016642.4 , NM_016642.3 | |
| Accession Version | NM_016642.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11025bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-02 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF233523.1, AC020659.5 and AA599654.1. On May 3, 2013 this sequence version replaced NM_016642.2. ##Evidence-Data-START## Transcript exon combination :: AF233523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_057726.4 |
| CDS | 229..11253 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_016642.3 |
1 | ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85485 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022299.1 | |
| Accession Version | XM_017022299.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11205bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877788.1 |
| CDS | 27..11231 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022299.1 |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85486 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022300.1 | |
| Accession Version | XM_017022300.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11085bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877789.1 |
| CDS | 27..11111 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022300.1 |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85487 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022301.1 | |
| Accession Version | XM_017022301.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10329bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTTTTTGG GGGGCTTCCT TACTCTCTGG ACACGATGGC AGAGGCTAAG GCCTGGAAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877790.1 |
| CDS | 354..10682 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022301.1 |
1 | ATGTTTTTGG GGGGCTTCCT TACTCTCTGG ACACGATGGC AGAGGCTAAG GCCTGGAAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85488 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022302.1 | |
| Accession Version | XM_017022302.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8382bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X4 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGAGTCT GCGGGCCCTG GCACAGCTCC GCAGGGCCCG GTTGGAGGAG GCCATGGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877791.1 |
| CDS | 99..8480 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022302.1 |
1 | ATGAGAGTCT GCGGGCCCTG GCACAGCTCC GCAGGGCCCG GTTGGAGGAG GCCATGGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85489 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022303.1 | |
| Accession Version | XM_017022303.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X5 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877792.1 |
| CDS | 27..7769 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022303.1 |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85490 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022304.1 | |
| Accession Version | XM_017022304.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X6 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877793.1 |
| CDS | 27..7136 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022304.1 |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu85491 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017022305.1 | |
| Accession Version | XM_017022305.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | spectrin beta chain, non-erythrocytic 5 isoform X7 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016877794.1 |
| CDS | 27..5597 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017022305.1 |
1 | ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Spectrin ?V adaptive mutations and changes in subcellular location correlate with emergence of hair cell electromotility in mammalians. |
The giant spectrin ?V couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route. |
Association of genetic variants with hemorrhagic stroke in Japanese individuals. |
Association of genetic variants with ischemic stroke in Japanese individuals with or without metabolic syndrome. |
Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals. |
