SPTBN5 cDNA ORF clone, Homo sapiens(human)

The following SPTBN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu15258 NM_016642.4
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu85485 XM_017022299.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu85486 XM_017022300.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu85487 XM_017022301.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu85488 XM_017022302.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu85489 XM_017022303.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu85490 XM_017022304.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu85491 XM_017022305.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
View Old Accession Versions >>
OHu15258 NM_016642.3 Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu15258
    Clone ID Related Accession (Same CDS sequence) NM_016642.4 , NM_016642.3
    Accession Version NM_016642.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF233523.1, AC020659.5 and AA599654.1. On Nov 22, 2018 this sequence version replaced NM_016642.3. ##Evidence-Data-START## Transcript exon combination :: AF233523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000320955.8/ ENSP00000317790.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
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    121
    181
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    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
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    3721
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
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    4621
    4681
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    4801
    4861
    4921
    4981
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    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
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    7921
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    8041
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    8161
    8221
    8281
    8341
    8401
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    8521
    8581
    8641
    8701
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    8821
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
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    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG 
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057726.4
    CDS205..11229
    Translation

    Target ORF information:

    RefSeq Version NM_016642.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016642.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG 
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15258
    Clone ID Related Accession (Same CDS sequence) NM_016642.4 , NM_016642.3
    Accession Version NM_016642.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF233523.1, AC020659.5 and AA599654.1. On May 3, 2013 this sequence version replaced NM_016642.2. ##Evidence-Data-START## Transcript exon combination :: AF233523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG 
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057726.4
    CDS229..11253
    Translation

    Target ORF information:

    RefSeq Version NM_016642.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016642.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG 
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85485
    Clone ID Related Accession (Same CDS sequence) XM_017022299.1
    Accession Version XM_017022299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2401
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    2521
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    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
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    7141
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    7261
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    7441
    7501
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    8401
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    8641
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGGT GGGCGGAAGC CACACCATCC
    GCGAAGGAGC AGAGAGAAGC CCCCTACAGA GATGGCAGGA GACTGCTCCA GCCTGGAAAG
    GCAGGGCTGC AGTCCAGACT GTCGCTCTCT TCCCACAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877788.1
    CDS27..11231
    Translation

    Target ORF information:

    RefSeq Version XM_017022299.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGGT GGGCGGAAGC CACACCATCC
    GCGAAGGAGC AGAGAGAAGC CCCCTACAGA GATGGCAGGA GACTGCTCCA GCCTGGAAAG
    GCAGGGCTGC AGTCCAGACT GTCGCTCTCT TCCCACAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85486
    Clone ID Related Accession (Same CDS sequence) XM_017022300.1
    Accession Version XM_017022300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    10501
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    11041
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877789.1
    CDS27..11111
    Translation

    Target ORF information:

    RefSeq Version XM_017022300.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGTCCCTAG AGCGTGAAGT GGGCCGCCTC TGCCAAAGAA GCCCCGAGGC AGCCCACGGC
    CTCAGGCACA GGCAGCAGGA GGTGGCTGAG AGCTGGTGGC AGCTCCGGAG CAGGGCCCAG
    AAGCGGAGGG AGGCGCTGGA TGCCTTGCAC CAAGCTCAGA AACTCCAGGC AATGCTGCAG
    GAATTGCTGG TCAGCGCCCA GAGGCTGCGG GCTCAGATGG ACACGAGCCC CGCTCCTCGC
    AGCCCTGTGG AAGCCCGGCG TATGTTAGAA GAGCATCAGG AGTGCAAGGC CGAGCTGGAC
    TCCTGGACAG ACAGCATCAG CCTGGCCCGA AGCACTGGGC AGCAACTGCT CACAGCGGGG
    CACCCCTTCA GCTCCGACAT TCGCCAGGTG CTGGCTGGCT TAGAACAGGA GCTGAGCAGC
    CTGGAAGGGG CCTGGCAGGA GCATCAGCTA CAGCTGCAGC AGGCCCTGGA GCTACAGCTG
    TTTCTGAGCT CAGTGGAGAA GATGGAACGT TGGCTTTGCA GCAAGGAAGA CTCCCTAGCC
    AGTGAGGGTC TATGGGACCC CTTGGCCCCC ATGGAGCCCC TTCTGTGGAA GCACAAGATG
    CTGGAGTGGG ACCTGGAGGT GCAGGCGGGA AAGATCAGTG CTCTGGAGGC CACGGCCCGC
    GGCCTGCACC AGGGTGGGCA CCCCGAGGCC CAGAGTGCCC TGGGCAGGTG CCAGGCCATG
    CTTCTGAGGA AGGAGGCGCT CTTCAGGCAA GCCGGGACCC GCCGCCATCG CCTGGAGGAG
    CTCCGGCAGC TGCAGGCCTT CCTGCAGGAC TCCCAGGAGG TGGCTGCGTG GCTGAGGGAG
    AAGAACCTGG TGGCCTTGGA GGAGGGTTTG CTGGACACAG CCATGCTGCC AGCACAGTTA
    CAGAAGCAGC AGAATTTCCA GGCGGAGCTG GACGCGAGCA TGCACCAACA GCAGGAGCTG
    CAGCGGGAGG GACAGAGGCT GCTGCAGGGG GGCCACCCAG CCTCGGAGGC CATCCAGGAG
    CGACTGGAGG AGCTGGGAGC ACTCTGGGGT GAGCTGCAAG ACAACTCCCA GAAGAAGGTG
    GCCAAGCTCC AGAAGGCCTG TGAGGCCCTG CGTCTGCGGC GGAGCATGGA GGAACTGGAG
    AACTGGCTGG AGCCCATCGA GGTTGAGCTG AGAGCCCCCA CTGTGGGCCA GGCCCTGCCT
    GGGGTGGGCG AGCTCCTGGG CACACAGAGG GAGCTGGAGG CAGCAGTGGA CAAGAAGGCC
    AGGCAGGCTG AGGCACTGCT GGGCCAGGCC CAGGCCTTTG TGAGGGAAGG CCACTGCCTT
    GCCCAAGATG TGGAAGAGCA GGCCCGGCGG CTGCTTCAGA GGTTCAAGAG CCTGAGGGAG
    CCCCTGCAGG AGCGCAGGAC GGCCCTGGAG GCCCGGAGCC TCCTCTTGAA GTTCTTCAGG
    GACGCCGACG AGGAAATGGC CTGGGTGCAG GAGAAGCTGC CTCTGGCCGC TGCCCAGGAC
    TATGGCCAGA GCCTGAGTGC GGTGCGGCAC CTGCAGGAGC AGCACCAGAA CCTGGAGAGT
    GAGATGAGCA GCCACGAGGC TCTGACCCGG GTGGTGCTGG GCACTGGGTA CAAGCTGGTG
    CAGGCTGGGC ACTTTGCCGC CCACGAGGTG GCCGCCCGGG TGCAGCAGCT GGAGAAGGCC
    ATGGCCCACC TGCGGGCAGA GGCGGCGCGG AGGCGGCTTC TGCTGCAGCA GGCTCAGGAG
    GCCCAGCAGT TTCTGACTGA GCTCCTGGAG GCGGGATCCT GGCTGGCTGA GCGGGGCCAT
    GTCCTGGACA GCGAGGACAT GGGCCACAGT GCTGAAGCCA CACAGGCCCT TCTGCGGCGG
    CTGGAGGCCA CCAAGAGAGA CCTGGAAGCG TTCAGCCCAC GCATCGAGCG GCTGCAGCAG
    ACAGCAGCAC TCCTGGAGAG CAGGAAGAAC CCAGAAAGCC CCAAGGTGCT AGCCCAGCTG
    CAGGCAGTTC GGGAGGCCCA CGCAGAGCTG CTGCGGAGGG CGGAGGCCAG GGGGCACGGC
    CTGCAGGAGC AGCTGCAGCT ACACCAGCTG GAGCGAGAGA CCCTGCTCCT CGACGCCTGG
    CTGACCACCA AGGCGGCCAC CGCCGAGTCC CAGGACTACG GGCAGGACCT GGAGGGTGTC
    AAGGTGCTGG AAGAGAAGTT TGATGCTTTC AGAAAGGAAG TGCAGAGCCT GGGCCAGGCC
    AAGGTGTATG CCCTGAGGAA GTTGGCAGGC ACCCTGGAGC GGGGTGCACC CAGGCGCTAT
    CCCCACATCC AAGCCCAGAG GAGCCGCATT GAGGCTGCTT GGGAGAGGTT GGACCAAGCA
    ATAAAAGCCC GCACAGAGAA CTTGGCTGCA GCCCATGAGG TCCACAGCTT TCAGCAGGCA
    GCAGCTGAGC TCCAGGGAAG GATGCAGGAG AAGACGGCCC TGATGAAGGG GGAGGACGGA
    GGCCACAGCC TGTCATCTGT GCGGACCCTG CAGCAACAGC ACAGGCGCCT GGAGAGAGAG
    CTGGAAGCTA TGGAGAAGGA GGTGGCACGG CTACAGACGG AGGCCTGCCG ACTGGGCCAG
    CTACATCCTG CAGCTCCGGG GGGCCTGGCC AAGGTGCAGG AGGCCTGGGC CACCCTGCAG
    GCGAAGGCCC AGGAGCGAGG CCAGTGGCTG GCGCAGGCTG CACAGGGCCA TGCCTTCCTC
    GGGCGCTGCC AGGAACTGCT AGCATGGGCA CAGGAGAGGC AGGAGCTGGC GTCCTCCGAG
    GAGCTGGCTG AGGACGTGGC GGGGGCTGAG CAGCTCCTTG GGCAGCATGA AGAGCTGGGG
    CAAGAAATCA GGGAGTGCCG CCTTCAAGCC CAGGACCTGC GGCAGGAAGG ACAGCAGCTG
    GTGGACAACA GCCACTTCAT GTCTGCGGAG GTGACAGAGT GCCTGCAGGA GCTGGAAGGG
    CGGCTGCAGG AGCTGGAGGA GGCTTGGGCC CTGCGCTGGC AACGCTGTGC CGAGAGCTGG
    GGCCTGCAGA AGCTTCGGCA GAGGCTGGAG CAGGCTGAGG CCTGGCTGGC CTGCTGGGAG
    GGACTCCTGC TGAAGCCCGA CTATGGGCAC TCAGTGTCAG ATGTGGAGTT GCTGCTGCAC
    AGACACCAGG ACTTAGAAAA GCTGCTGGCA GCCCAGGAAG AGAAGTTTGC CCAAATGCAA
    AAGACAGAGA TGGAACAGGA GCTCCTGCTG CAGCCACAGG AGCTGAAGCC CGGGAGAGCT
    GGCAGCTCGC TGACATCCTT TCAGTGGAGG CCCTCTGGAC ACCAGGGGCT AGGAGCACAG
    CTGGCTGAGA CGAGGGACCC CCAGGATGCA AAGGGTACCC CCACCATGGA GGGGTCTTTG
    GAGTTCAAGC AGCACCTGCT GCCTGGCGGG AGGCAGCCTA GCTCGAGCTC CTGGGACAGC
    TGCCGCGGGA ACTTGCAGGG CAGCTCTCTG AGCCTGTTCC TGGATGAGAG GATGGCAGCG
    GAGAAAGTAG CTTCCATAGC CCTCCTTGAC CTCACGGGAG CCCGGTGTGA GAGGCTGCGG
    GGCCGCCACG GCAGGAAACA CACATTCTCC TTAAGGCTGA CCAGTGGGGC AGAGATCCTG
    TTTGCAGCAC CGTCCGAAGA GCAGGCTGAG AGCTGGTGGC GAGCCCTGGG CAGCACTGCA
    GCCCAGAGTC TGAGCCCAAA ACTCAAAGCC AAACCTGTCA GCTCTCTGAA TGAGTGCACG
    ACCAAGGATG CCCGGCCTGG ATGTCTACTC AGGTCTGATC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85487
    Clone ID Related Accession (Same CDS sequence) XM_017022301.1
    Accession Version XM_017022301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
    1441
    1501
    1561
    1621
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    1741
    1801
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    1921
    1981
    2041
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    2641
    2701
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    2941
    3001
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    3181
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    4141
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    4261
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    4441
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    4681
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    5941
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    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    ATGTTTTTGG GGGGCTTCCT TACTCTCTGG ACACGATGGC AGAGGCTAAG GCCTGGAAGT 
    GCTCTTCTCA AGATCCTGCT TCAGCTCCAG GAGACAGAGC TGCTGCAGAC CCAGTACGAG
    CAGCTGGTGG CTGACCTTCT ACGCTGGATT GCAGAGAAGC AGATGCAGCT GGAGGCGCGG
    GATTTTCCAG ACTCGCTGCC CGCCATGCGG CAGCTACTGG CAGCATTCAC CATCTTCCGC
    ACCCAGGAGA AGCCACCCCG GCTACAGCAG CGAGGGGCCG CAGAGGCCCT GCTCTTCCGG
    CTACAGACAG CACTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCTCATGA GGGCCTGGGC
    CTTGCAGAGC TGTCCCAGTG CTGGGCAGGG CTGGAGTGGG CAGAGGCTGC AAGGAGCCAG
    GCCCTGCAGC AGAGGCTACT GCAGCTGCAG CGGCTAGAAA CCCTGGCCCG GCGCTTCCAG
    CACAAGGCAG CCCTCCGGGA GAGTTTCCTT AAGGATGCAG AGCAGGTGCT AGACCAGGCC
    AGAGCCCCGC CAGCCAGCCT GGCCACAGTG GAGGCAGCCG TCCAGAGGCT GGGCATGCTG
    GAGGCTGGCA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCTGAGAT CGCAGACATC
    CTCCGGCAGG AGCAGTACCA CAGCTGGGCA GATGTGGCCC GCAGGCAGGA GGAAGTTACC
    GTGCGCTGGC AGAGGCTCCT TCAGCATCTA CAGGGACAGA GGAAGCAGGT GGCAGACATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGGCTGCCT CCCACCAGCT GGAGGAGCTG
    CAGGAGCCGG CCAGGTCCAC CGCCTGTGGG CAGCAGCTGG CAGAAGTGGT GGAGCTGCTG
    CAGAGGCATG ACCTGCTGGA GGCTCAAGTC TCGGCCCACG GAGCCCATGT GAGCCATCTT
    GCTCAGCAGA CAGCAGAGCT GGACTCCTCC CTGGGCACCA GTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGA CACTGGCCCA GCTCCAACAG AGCCTGGTGG CTCTTGTCAG GGCCCGGCGG
    GCCCTGCTGG AGCAGACCCT GCAGCGGGCA GAGTTCCTGC GCAACTGTGA GGAGGAGGAA
    GCCTGGCTGA AGGAGTGCGG ACAGCGGGTG GGGAATGCGG CCCTGGGCCG GGATCTCAGC
    CAGATCGCAG GCGCCCTGCA GAAACACAAG GCCCTGGAAG CTGAGGTCCA CCGCCACCAG
    GCCGTGTGCG TAGATCTCGT GCGGAGGGGA CGCGACCTCA GCGCCCGCAG GCCCCCAACG
    CAGCCGGATC CCGGGGAACG GGCAGAGGCC GTTCAGGGAG GGTGGCAGCT GCTCCAGACC
    CGGGTGGTGG GGCGGGGCGC ACGGCTGCAG ACAGCCCTGC TGGTCCTGCA GTACTTCGCG
    GACGCGGCGG AGGCGGCTTC GTGGCTGCGC GAGCGGCGAT CCTCGCTGGA GAGAGCGTCC
    TGCGGTCAGG ACCAGGCGGC CGCCGAGACC CTGCTGAGGC GCCACGTGCG GCTGGAGCGC
    GTCCTGCGCG CCTTCGCGGC CGAGCTGCGG CGGCTGGAGG AGCAGGGGCG GGCGGCCTCG
    GCCCGGGCGT CGTTATTCAC GGTGAACTCT GCCCTGAGCC CTCCAGGAGA AAGCCTGAGG
    AACCCAGGGC CCTGGAGTGA GGCTTCCTGC CACCCTGGCC CTGGGGATGC CTGGAAGATG
    GCCCTCCCAG CTGAGCCTGA CCCTGACTTT GATCCCAACA CTATACTCCA GACACAGGAC
    CACTTGAGTC AGGACTATGA GAGTCTGCGG GCCCTGGCAC AGCTCCGCAG GGCCCGGTTG
    GAGGAGGCCA TGGCCCTGTT CGGTTTCTGC AGTTCCTGTG GGGAGCTCCA GTTGTGGCTG
    GAGAAGCAGA CAGTGCTGCT CCAAAGGGTG CAGCCCCAGG CTGACACCCT GGAGGTCATG
    CAGCTCAAAT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAAGGGCCT CTGGGCTGAG
    GTCAGCAGCT CTGCTGAGCA ACTGAGGCAG AGATATCCTG GGAACTCCAC ACAAATCCAA
    CGACAGCAGG AGGAACTGAG CCAGAGGTGG GGGCAGCTGG AGGCCCTGAA GAGGGAGAAG
    GCCGTGCAGC TGGCACACAG TGTGGAAGTG TGCAGTTTTC TGCAGGAGTG TGGACCCACA
    CAGGTCCAGC TGCGAGACGT GCTCCTCCAG CTGGAGGCCC TGCAGCCAGG GAGCTCAGAG
    GACACCTGCC ACGCCCTGCA GCTGGCCCAG AAGAAGACCC TGGTGCTGGA GAGGAGGGTC
    CACTTCCTCC AAAGTGTGGT CGTAAAGGTC GAGGAGCCAG GCTACGCAGA GAGCCAGCCT
    CTGCAAGGAC AGGTGGAGAC ACTGCAGGGG CTGCTGAAGC AAGTACAGGA ACAAGTGGCC
    CAACGGGCCC GGCGCCAGGC TGAGACTCAG GCCCGGCAGA GCTTCCTGCA AGAGAGCCAG
    CAACTGCTAC TGTGGGCAGA GAGTGTCCAG GCTCAGCTGC GCAGCAAGGA GGTGTCAGTG
    GATGTGGCCT CGGCTCAGCG GCTGCTGAGG GAGCACCAAG ACCTGCTGGA GGAGATCCAC
    CTGTGGCAGG AGAGGCTGCA GCAGCTGGAC GCTCAGAGCC AGCCCATGGC AGCCTTGGAC
    TGCCCAGACT CCCAAGAGGT GCCCAACACT CTGAGGGTCC TGGGGCAGCA GGGCCAGGAG
    CTGAAGGTTT TGTGGGAGCA GAGGCAGCAG TGGCTGCAAG AGGGGCTGGA GCTGCAGAAG
    TTTGGCCGAG AAGTGGATGG TTTCACTGCC ACCTGTGCCA ACCACCAGGC CTGGCTGCAC
    CTGGACAACC TTGGGGAGGA CGTGAGGGAG GCCCTGAGCC TGCTGCAGCA GCACCGGGAG
    TTTGGGCGGC TCCTGAGCAC CCTGGGGCCT CGGGCAGAGG CTCTGCGGGC ACACGGCGAG
    AAGCTGGTTC AGAGCCAGCA CCCAGCTGCA CACACGGTCA GAGAGCAGCT GCAGAGTATC
    CAGGCACAGT GGACCAGGCT CCAGGGGAGG AGTGAGCAGA GGAGGAGGCA GTTGCTGGCT
    TCCCTCCAGC TCCAGGAGTG GAAGCAGGAT GTGGCAGAGC TGATGCAGTG GATGGAAGAG
    AAGGGGCTGA TGGCTGCGCA TGAGCCCTCC GGAGCGCGCA GAAACATCCT GCAGACACTC
    AAGCGGCACG AAGCAGCTGA GAGCGAGCTA CTCGCCACCC GCAGACACGT GGAGGCCCTG
    CAGCAGGTTG GGAGAGAGCT GTTGAGTAGG AGGCCCTGTG GCCAGGAGGA CATACAGACC
    AGGCTTCAAG GCCTGAGAAG CAAGTGGGAA GCTTTGAACC GCAAGATGAC TGAGCGTGGG
    GACGAGCTCC AGCAGGCTGG ACAGCAGGAG CAACTCCTGA GGCAGCTGCA GGATGCAAAG
    GAGCAGCTGG AGCAGCTCGA AGGGGCCCTA CAGAGCTCGG AAACAGGGCA GGACCTGCGC
    TCCAGCCAGA GGCTGCAGAA ACGGCACCAA CAGCTGGAGA GTGAGAGCCG GACCCTGGCT
    GCCAAGATGG CTGCCCTCGC CTCCATGGCC CATGGCATGG CCGCCTCCCC GGCCATCCTG
    GAAGAGACCC AGAAGCACCT CCGGAGGCTG GAGCTTCTGC AGGGGCATCT GGCCATCCGG
    GGCCTGCAGC TGCAGGCCTC AGTGGAGCTG CACCAGTTCT GCCACCTGAG CAACATGGAG
    CTCTCTTGGG TAGCCGAGCA CATGCCCCAT GGCAGCCCCA CCAGCTATAC CGAGTGCTTG
    AATGGTGCCC AGAGCCTTCA CCGCAAGCAC AAGGAGCTCC AGGTGGAGGT AAAAGCTCAC
    CAGGGGCAGG TGCAACGGGT GCTGAGTTCT GGGCGGAGCC TGGCAGCCTC AGGGCACCCC
    CAAGCCCAAC ACATCGTGGA GCAGTGCCAG GAGCTGGAAG GCCACTGGGC AGAGCTGGAG
    AGGGCATGTG AAGCGCGGGC CCAGTGTCTG CAGCAGGCTG TCACTTTCCA GCAGTACTTT
    CTGGATGTGT CAGAGCTGGA GGGCTGGGTG GAGGAGAAGC GGCCGCTGGT GAGCAGTCGG
    GACTATGGCA GAGACGAGGC AGCCACCCTC AGGCTCATTA ACAAGCACCA GGCTCTACAG
    GAGGAACTAG CCATTTACTG GAGCTCCATG GAGGAGCTTG ACCAGACGGC CCAAACCCTC
    ACTGGCCCCG AAGTCCCTGA GCAGCAGCGT GTGGTGCAGG AGAGGCTCCG GGAGCAGCTG
    CGGGCACTGC AGGAGTTGGC GGCCACACGG GACCGGGAAC TGGAGGGGAC CCTGAGGCTG
    CATGAGTTCC TGAGGGAGGC TGAGGACCTG CAGGGCTGGC TGGCAAGCCA GAAGCAGGCA
    GCCAAAGGAG GGGAGAGCCT GGGAGAGGAC CCCGAGCACG CCCTGCACCT CTGCACCAAG
    TTTGCAAAGT TTCAGCACCA AGTGGAGATG GGCAGCCAGC GGGTGGCCGC CTGCCGGCTG
    CTGGCGGAGA GCCTGCTAGA GCGTGGGCAC AGTGCTGGCC CCATGGTCCG TCAGAGGCAG
    CAGGATCTGC AGACCGCCTG GTCGGAGCTG TGGGAGCTGA CCCAGGCCCG AGGCCACGCG
    CTCCGAGACA CCGAGACCAC CCTCAGAGTT CACAGAGATC TCTTGGAAGT CCTCACCCAG
    GTCCAGGAGA AAGCCACGAG CCTCCCCAAC AATGTGGCAC GGGACCTGTG TGGGCTGGAG
    GCGCAGCTGA GAAGCCACCA GGGGCTGGAG CGAGAACTCG TGGGCACCGA GCGGCAGCTG
    CAGGAACTGC TGGAGACTGC AGGCAGGGTG CAGAAGCTGT GTCCGGGGCC TCAGGCCCAT
    GCGGTGCAGC AGAGGCAGCA AGCTGTGACG CAGGCGTGGG CAGTGCTGCA GCGACGCATG
    GAGCAGCGCA GGGCCCAGCT GGAGCGGGCA CGCCTCCTGG CCCGCTTCCG CACGGCGGTG
    CGTGACTATG CCTCCTGGGC AGCCCGCGTG CGCCAGGACC TGCAGGTGGA GGAGAGTTCG
    CAAGAGCCTA GCAGTGGCCC GCTGAAGCTC AGTGCCCACC AGTGGCTCCG GGCGGAGCTG
    GAGGCCCGGG AGAAGCTGTG GCAGCAGGCC ACCCAGCTGG GGCAGCAGGC ACTTCTTGCT
    GCAGGGACAC CCACCAAGGA GGTCCAGGAA GAGCTTCGAG CCCTGCAGGA CCAGCGGGAC
    CAGGTGTATC AGACCTGGGC ACGGAAGCAA GAGAGGCTGC AGGCCGAGCA GCAGGAGCAG
    CTCTTCCTCA GAGAGTGCGG CCGCCTGGAG GAGATCCTCG CGGCCCAGGA GGTCTCCCTG
    AAAACCAGTG CCTTGGGGAG CTCGGTGGAA GAGGTAGAGC AGTTGATTCG CAAGCACGAG
    GTCTTCCTGA AGGTTCTGAC TGCCCAGGAC AAGAAGGAGG CAGCCCTGCG TGAGCGGCTG
    AAGACGCTCC GGCGCCCCCG GGTGCGGGAC CGGCTTCCCA TCCTGCTGCA GCGCCGGATG
    AGAGTGAAGG AGCTGGCGGA GAGCCGGGGA CACGCCCTGC ATGCCTCCCT GCTGATGGCC
    AGCTTCACCC AGGCCGCAAC CCAGGCTGAG GACTGGATCC AGGCGTGGGC CCAGCAGCTG
    AAGGAGCCGG TCCCTCCTGG GGACCTGAGA GATAAGCTGA AGCCCCTGCT GAAACACCAG
    GCCTTTGAGG CTGAAGTCCA GGCCCATGAG GAGGTCATGA CCTCTGTTGC CAAGAAGGGA
    GAGGCTCTCC TGGCACAGAG TCACCCTCGA GCCGGAGAGG TCTCCCAGCG GCTGCAGGGC
    CTGCGGAAGC ACTGGGAGGA CCTGAGGCAG GCAATGGCCC TCAGGGGCCA GGAGCTGGAG
    GACAGGCGGA ACTTCCTGGA GTTCCTGCAG AGAGTGGACC TTGCAGAGGC CTGGATCCAG
    GAGAAGGAGG TGAAGATGAA TGTTGGTGAC CTGGGCCAGG ACCTGGAGCA CTGCCTGCAG
    CTCCGACGGC GGCTCCGCGA GTTCCGAGGA AACTCGGCCG GGGACACAGT GGGTGATGCC
    TGCATCAGGA GCATCAGTGA CTTGTCACTG CAGCTCAAGA ACCGGGACCC TGAGGAAGTC
    AAGATCATCT GCCAGCGGCG AAGCCAGCTC AACAACAGGT GGGCGAGTTT CCATGGCAAC
    TTGCTCCGGT ACCAGCAGCA GCTCGAAGGG GCCTTGGAGA TACACGTGTT GTCCCGAGAG
    CTGGACAATG TCACCAAGAG GATTCAGGAG AAGGAAGCCC TGATCCAGGC CCTGGACTGT
    GGGAAAGATC TGGAGAGCGT GCAGAGGCTG CTGCGGAAAC ACGAGGAGCT GGAGCGGGAA
    GTGCACCCCA TCCAGGCCCA GGTGGAGTCC CTAGAGCGTG AAGTGGGCCG CCTCTGCCAA
    AGAAGCCCCG AGGCAGCCCA CGGCCTCAGG CACAGGCAGC AGGAGGTGGC TGAGAGCTGG
    TGGCAGCTCC GGAGCAGGGC CCAGAAGCGG AGGGAGGCGC TGGATGCCTT GCACCAAGCT
    CAGAAACTCC AGGCAATGCT GCAGGAATTG CTGGTCAGCG CCCAGAGGCT GCGGGCTCAG
    ATGGACACGA GCCCCGCTCC TCGCAGCCCT GTGGAAGCCC GGCGTATGTT AGAAGAGCAT
    CAGGAGTGCA AGGCCGAGCT GGACTCCTGG ACAGACAGCA TCAGCCTGGC CCGAAGCACT
    GGGCAGCAAC TGCTCACAGC GGGGCACCCC TTCAGCTCCG ACATTCGCCA GGTGCTGGCT
    GGCTTAGAAC AGGAGCTGAG CAGCCTGGAA GGGGCCTGGC AGGAGCATCA GCTACAGCTG
    CAGCAGGCCC TGGAGCTACA GCTGTTTCTG AGCTCAGTGG AGAAGATGGA ACGTTGGCTT
    TGCAGCAAGG AAGACTCCCT AGCCAGTGAG GGTCTATGGG ACCCCTTGGC CCCCATGGAG
    CCCCTTCTGT GGAAGCACAA GATGCTGGAG TGGGACCTGG AGGTGCAGGC GGGAAAGATC
    AGTGCTCTGG AGGCCACGGC CCGCGGCCTG CACCAGGGTG GGCACCCCGA GGCCCAGAGT
    GCCCTGGGCA GGTGCCAGGC CATGCTTCTG AGGAAGGAGG CGCTCTTCAG GCAAGCCGGG
    ACCCGCCGCC ATCGCCTGGA GGAGCTCCGG CAGCTGCAGG CCTTCCTGCA GGACTCCCAG
    GAGGTGGCTG CGTGGCTGAG GGAGAAGAAC CTGGTGGCCT TGGAGGAGGG TTTGCTGGAC
    ACAGCCATGC TGCCAGCACA GTTACAGAAG CAGCAGAATT TCCAGGCGGA GCTGGACGCG
    AGCATGCACC AACAGCAGGA GCTGCAGCGG GAGGGACAGA GGCTGCTGCA GGGGGGCCAC
    CCAGCCTCGG AGGCCATCCA GGAGCGACTG GAGGAGCTGG GAGCACTCTG GGGTGAGCTG
    CAAGACAACT CCCAGAAGAA GGTGGCCAAG CTCCAGAAGG CCTGTGAGGC CCTGCGTCTG
    CGGCGGAGCA TGGAGGAACT GGAGAACTGG CTGGAGCCCA TCGAGGTTGA GCTGAGAGCC
    CCCACTGTGG GCCAGGCCCT GCCTGGGGTG GGCGAGCTCC TGGGCACACA GAGGGAGCTG
    GAGGCAGCAG TGGACAAGAA GGCCAGGCAG GCTGAGGCAC TGCTGGGCCA GGCCCAGGCC
    TTTGTGAGGG AAGGCCACTG CCTTGCCCAA GATGTGGAAG AGCAGGCCCG GCGGCTGCTT
    CAGAGGTTCA AGAGCCTGAG GGAGCCCCTG CAGGAGCGCA GGACGGCCCT GGAGGCCCGG
    AGCCTCCTCT TGAAGTTCTT CAGGGACGCC GACGAGGAAA TGGCCTGGGT GCAGGAGAAG
    CTGCCTCTGG CCGCTGCCCA GGACTATGGC CAGAGCCTGA GTGCGGTGCG GCACCTGCAG
    GAGCAGCACC AGAACCTGGA GAGTGAGATG AGCAGCCACG AGGCTCTGAC CCGGGTGGTG
    CTGGGCACTG GGTACAAGCT GGTGCAGGCT GGGCACTTTG CCGCCCACGA GGTGGCCGCC
    CGGGTGCAGC AGCTGGAGAA GGCCATGGCC CACCTGCGGG CAGAGGCGGC GCGGAGGCGG
    CTTCTGCTGC AGCAGGCTCA GGAGGCCCAG CAGTTTCTGA CTGAGCTCCT GGAGGCGGGA
    TCCTGGCTGG CTGAGCGGGG CCATGTCCTG GACAGCGAGG ACATGGGCCA CAGTGCTGAA
    GCCACACAGG CCCTTCTGCG GCGGCTGGAG GCCACCAAGA GAGACCTGGA AGCGTTCAGC
    CCACGCATCG AGCGGCTGCA GCAGACAGCA GCACTCCTGG AGAGCAGGAA GAACCCAGAA
    AGGTGGGCGG AAGCCACACC ATCCGCGAAG GAGCAGAGAG AAGCCCCCTA CAGAGATGGC
    AGGAGACTGC TCCAGCCTGG AAAGGCAGGG CTGCAGTCCA GACTGTCGCT CTCTTCCCAC
    AGCCCCAAGG TGCTAGCCCA GCTGCAGGCA GTTCGGGAGG CCCACGCAGA GCTGCTGCGG
    AGGGCGGAGG CCAGGGGGCA CGGCCTGCAG GAGCAGCTGC AGCTACACCA GCTGGAGCGA
    GAGACCCTGC TCCTCGACGC CTGGCTGACC ACCAAGGCGG CCACCGCCGA GTCCCAGGAC
    TACGGGCAGG ACCTGGAGGG TGTCAAGGTG CTGGAAGAGA AGTTTGATGC TTTCAGAAAG
    GAAGTGCAGA GCCTGGGCCA GGCCAAGGTG TATGCCCTGA GGAAGTTGGC AGGCACCCTG
    GAGCGGGGTG CACCCAGGCG CTATCCCCAC ATCCAAGCCC AGAGGAGCCG CATTGAGGCT
    GCTTGGGAGA GGTTGGACCA AGCAATAAAA GCCCGCACAG AGAACTTGGC TGCAGCCCAT
    GAGGTCCACA GCTTTCAGCA GGCAGCAGCT GAGCTCCAGG GAAGGATGCA GGAGAAGACG
    GCCCTGATGA AGGGGGAGGA CGGAGGCCAC AGCCTGTCAT CTGTGCGGAC CCTGCAGCAA
    CAGCACAGGC GCCTGGAGAG AGAGCTGGAA GCTATGGAGA AGGAGGTGGC ACGGCTACAG
    ACGGAGGCCT GCCGACTGGG CCAGCTACAT CCTGCAGCTC CGGGGGGCCT GGCCAAGGTG
    CAGGAGGCCT GGGCCACCCT GCAGGCGAAG GCCCAGGAGC GAGGCCAGTG GCTGGCGCAG
    GCTGCACAGG GCCATGCCTT CCTCGGGCGC TGCCAGGAAC TGCTAGCATG GGCACAGGAG
    AGGCAGGAGC TGGCGTCCTC CGAGGAGCTG GCTGAGGACG TGGCGGGGGC TGAGCAGCTC
    CTTGGGCAGC ATGAAGAGCT GGGGCAAGAA ATCAGGGAGT GCCGCCTTCA AGCCCAGGAC
    CTGCGGCAGG AAGGACAGCA GCTGGTGGAC AACAGCCACT TCATGTCTGC GGAGGTGACA
    GAGTGCCTGC AGGAGCTGGA AGGGCGGCTG CAGGAGCTGG AGGAGGCTTG GGCCCTGCGC
    TGGCAACGCT GTGCCGAGAG CTGGGGCCTG CAGAAGCTTC GGCAGAGGCT GGAGCAGGCT
    GAGGCCTGGC TGGCCTGCTG GGAGGGACTC CTGCTGAAGC CCGACTATGG GCACTCAGTG
    TCAGATGTGG AGTTGCTGCT GCACAGACAC CAGGACTTAG AAAAGCTGCT GGCAGCCCAG
    GAAGAGAAGT TTGCCCAAAT GCAAAAGACA GAGATGGAAC AGGAGCTCCT GCTGCAGCCA
    CAGGAGCTGA AGCCCGGGAG AGCTGGCAGC TCGCTGACAT CCTTTCAGTG GAGGCCCTCT
    GGACACCAGG GGCTAGGAGC ACAGCTGGCT GAGACGAGGG ACCCCCAGGA TGCAAAGGGT
    ACCCCCACCA TGGAGGGGTC TTTGGAGTTC AAGCAGCACC TGCTGCCTGG CGGGAGGCAG
    CCTAGCTCGA GCTCCTGGGA CAGCTGCCGC GGGAACTTGC AGGGCAGCTC TCTGAGCCTG
    TTCCTGGATG AGAGGATGGC AGCGGAGAAA GTAGCTTCCA TAGCCCTCCT TGACCTCACG
    GGAGCCCGGT GTGAGAGGCT GCGGGGCCGC CACGGCAGGA AACACACATT CTCCTTAAGG
    CTGACCAGTG GGGCAGAGAT CCTGTTTGCA GCACCGTCCG AAGAGCAGGC TGAGAGCTGG
    TGGCGAGCCC TGGGCAGCAC TGCAGCCCAG AGTCTGAGCC CAAAACTCAA AGCCAAACCT
    GTCAGCTCTC TGAATGAGTG CACGACCAAG GATGCCCGGC CTGGATGTCT ACTCAGGTCT
    GATCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877790.1
    CDS354..10682
    Translation

    Target ORF information:

    RefSeq Version XM_017022301.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    ATGTTTTTGG GGGGCTTCCT TACTCTCTGG ACACGATGGC AGAGGCTAAG GCCTGGAAGT 
    GCTCTTCTCA AGATCCTGCT TCAGCTCCAG GAGACAGAGC TGCTGCAGAC CCAGTACGAG
    CAGCTGGTGG CTGACCTTCT ACGCTGGATT GCAGAGAAGC AGATGCAGCT GGAGGCGCGG
    GATTTTCCAG ACTCGCTGCC CGCCATGCGG CAGCTACTGG CAGCATTCAC CATCTTCCGC
    ACCCAGGAGA AGCCACCCCG GCTACAGCAG CGAGGGGCCG CAGAGGCCCT GCTCTTCCGG
    CTACAGACAG CACTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCTCATGA GGGCCTGGGC
    CTTGCAGAGC TGTCCCAGTG CTGGGCAGGG CTGGAGTGGG CAGAGGCTGC AAGGAGCCAG
    GCCCTGCAGC AGAGGCTACT GCAGCTGCAG CGGCTAGAAA CCCTGGCCCG GCGCTTCCAG
    CACAAGGCAG CCCTCCGGGA GAGTTTCCTT AAGGATGCAG AGCAGGTGCT AGACCAGGCC
    AGAGCCCCGC CAGCCAGCCT GGCCACAGTG GAGGCAGCCG TCCAGAGGCT GGGCATGCTG
    GAGGCTGGCA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCTGAGAT CGCAGACATC
    CTCCGGCAGG AGCAGTACCA CAGCTGGGCA GATGTGGCCC GCAGGCAGGA GGAAGTTACC
    GTGCGCTGGC AGAGGCTCCT TCAGCATCTA CAGGGACAGA GGAAGCAGGT GGCAGACATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGGCTGCCT CCCACCAGCT GGAGGAGCTG
    CAGGAGCCGG CCAGGTCCAC CGCCTGTGGG CAGCAGCTGG CAGAAGTGGT GGAGCTGCTG
    CAGAGGCATG ACCTGCTGGA GGCTCAAGTC TCGGCCCACG GAGCCCATGT GAGCCATCTT
    GCTCAGCAGA CAGCAGAGCT GGACTCCTCC CTGGGCACCA GTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGA CACTGGCCCA GCTCCAACAG AGCCTGGTGG CTCTTGTCAG GGCCCGGCGG
    GCCCTGCTGG AGCAGACCCT GCAGCGGGCA GAGTTCCTGC GCAACTGTGA GGAGGAGGAA
    GCCTGGCTGA AGGAGTGCGG ACAGCGGGTG GGGAATGCGG CCCTGGGCCG GGATCTCAGC
    CAGATCGCAG GCGCCCTGCA GAAACACAAG GCCCTGGAAG CTGAGGTCCA CCGCCACCAG
    GCCGTGTGCG TAGATCTCGT GCGGAGGGGA CGCGACCTCA GCGCCCGCAG GCCCCCAACG
    CAGCCGGATC CCGGGGAACG GGCAGAGGCC GTTCAGGGAG GGTGGCAGCT GCTCCAGACC
    CGGGTGGTGG GGCGGGGCGC ACGGCTGCAG ACAGCCCTGC TGGTCCTGCA GTACTTCGCG
    GACGCGGCGG AGGCGGCTTC GTGGCTGCGC GAGCGGCGAT CCTCGCTGGA GAGAGCGTCC
    TGCGGTCAGG ACCAGGCGGC CGCCGAGACC CTGCTGAGGC GCCACGTGCG GCTGGAGCGC
    GTCCTGCGCG CCTTCGCGGC CGAGCTGCGG CGGCTGGAGG AGCAGGGGCG GGCGGCCTCG
    GCCCGGGCGT CGTTATTCAC GGTGAACTCT GCCCTGAGCC CTCCAGGAGA AAGCCTGAGG
    AACCCAGGGC CCTGGAGTGA GGCTTCCTGC CACCCTGGCC CTGGGGATGC CTGGAAGATG
    GCCCTCCCAG CTGAGCCTGA CCCTGACTTT GATCCCAACA CTATACTCCA GACACAGGAC
    CACTTGAGTC AGGACTATGA GAGTCTGCGG GCCCTGGCAC AGCTCCGCAG GGCCCGGTTG
    GAGGAGGCCA TGGCCCTGTT CGGTTTCTGC AGTTCCTGTG GGGAGCTCCA GTTGTGGCTG
    GAGAAGCAGA CAGTGCTGCT CCAAAGGGTG CAGCCCCAGG CTGACACCCT GGAGGTCATG
    CAGCTCAAAT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAAGGGCCT CTGGGCTGAG
    GTCAGCAGCT CTGCTGAGCA ACTGAGGCAG AGATATCCTG GGAACTCCAC ACAAATCCAA
    CGACAGCAGG AGGAACTGAG CCAGAGGTGG GGGCAGCTGG AGGCCCTGAA GAGGGAGAAG
    GCCGTGCAGC TGGCACACAG TGTGGAAGTG TGCAGTTTTC TGCAGGAGTG TGGACCCACA
    CAGGTCCAGC TGCGAGACGT GCTCCTCCAG CTGGAGGCCC TGCAGCCAGG GAGCTCAGAG
    GACACCTGCC ACGCCCTGCA GCTGGCCCAG AAGAAGACCC TGGTGCTGGA GAGGAGGGTC
    CACTTCCTCC AAAGTGTGGT CGTAAAGGTC GAGGAGCCAG GCTACGCAGA GAGCCAGCCT
    CTGCAAGGAC AGGTGGAGAC ACTGCAGGGG CTGCTGAAGC AAGTACAGGA ACAAGTGGCC
    CAACGGGCCC GGCGCCAGGC TGAGACTCAG GCCCGGCAGA GCTTCCTGCA AGAGAGCCAG
    CAACTGCTAC TGTGGGCAGA GAGTGTCCAG GCTCAGCTGC GCAGCAAGGA GGTGTCAGTG
    GATGTGGCCT CGGCTCAGCG GCTGCTGAGG GAGCACCAAG ACCTGCTGGA GGAGATCCAC
    CTGTGGCAGG AGAGGCTGCA GCAGCTGGAC GCTCAGAGCC AGCCCATGGC AGCCTTGGAC
    TGCCCAGACT CCCAAGAGGT GCCCAACACT CTGAGGGTCC TGGGGCAGCA GGGCCAGGAG
    CTGAAGGTTT TGTGGGAGCA GAGGCAGCAG TGGCTGCAAG AGGGGCTGGA GCTGCAGAAG
    TTTGGCCGAG AAGTGGATGG TTTCACTGCC ACCTGTGCCA ACCACCAGGC CTGGCTGCAC
    CTGGACAACC TTGGGGAGGA CGTGAGGGAG GCCCTGAGCC TGCTGCAGCA GCACCGGGAG
    TTTGGGCGGC TCCTGAGCAC CCTGGGGCCT CGGGCAGAGG CTCTGCGGGC ACACGGCGAG
    AAGCTGGTTC AGAGCCAGCA CCCAGCTGCA CACACGGTCA GAGAGCAGCT GCAGAGTATC
    CAGGCACAGT GGACCAGGCT CCAGGGGAGG AGTGAGCAGA GGAGGAGGCA GTTGCTGGCT
    TCCCTCCAGC TCCAGGAGTG GAAGCAGGAT GTGGCAGAGC TGATGCAGTG GATGGAAGAG
    AAGGGGCTGA TGGCTGCGCA TGAGCCCTCC GGAGCGCGCA GAAACATCCT GCAGACACTC
    AAGCGGCACG AAGCAGCTGA GAGCGAGCTA CTCGCCACCC GCAGACACGT GGAGGCCCTG
    CAGCAGGTTG GGAGAGAGCT GTTGAGTAGG AGGCCCTGTG GCCAGGAGGA CATACAGACC
    AGGCTTCAAG GCCTGAGAAG CAAGTGGGAA GCTTTGAACC GCAAGATGAC TGAGCGTGGG
    GACGAGCTCC AGCAGGCTGG ACAGCAGGAG CAACTCCTGA GGCAGCTGCA GGATGCAAAG
    GAGCAGCTGG AGCAGCTCGA AGGGGCCCTA CAGAGCTCGG AAACAGGGCA GGACCTGCGC
    TCCAGCCAGA GGCTGCAGAA ACGGCACCAA CAGCTGGAGA GTGAGAGCCG GACCCTGGCT
    GCCAAGATGG CTGCCCTCGC CTCCATGGCC CATGGCATGG CCGCCTCCCC GGCCATCCTG
    GAAGAGACCC AGAAGCACCT CCGGAGGCTG GAGCTTCTGC AGGGGCATCT GGCCATCCGG
    GGCCTGCAGC TGCAGGCCTC AGTGGAGCTG CACCAGTTCT GCCACCTGAG CAACATGGAG
    CTCTCTTGGG TAGCCGAGCA CATGCCCCAT GGCAGCCCCA CCAGCTATAC CGAGTGCTTG
    AATGGTGCCC AGAGCCTTCA CCGCAAGCAC AAGGAGCTCC AGGTGGAGGT AAAAGCTCAC
    CAGGGGCAGG TGCAACGGGT GCTGAGTTCT GGGCGGAGCC TGGCAGCCTC AGGGCACCCC
    CAAGCCCAAC ACATCGTGGA GCAGTGCCAG GAGCTGGAAG GCCACTGGGC AGAGCTGGAG
    AGGGCATGTG AAGCGCGGGC CCAGTGTCTG CAGCAGGCTG TCACTTTCCA GCAGTACTTT
    CTGGATGTGT CAGAGCTGGA GGGCTGGGTG GAGGAGAAGC GGCCGCTGGT GAGCAGTCGG
    GACTATGGCA GAGACGAGGC AGCCACCCTC AGGCTCATTA ACAAGCACCA GGCTCTACAG
    GAGGAACTAG CCATTTACTG GAGCTCCATG GAGGAGCTTG ACCAGACGGC CCAAACCCTC
    ACTGGCCCCG AAGTCCCTGA GCAGCAGCGT GTGGTGCAGG AGAGGCTCCG GGAGCAGCTG
    CGGGCACTGC AGGAGTTGGC GGCCACACGG GACCGGGAAC TGGAGGGGAC CCTGAGGCTG
    CATGAGTTCC TGAGGGAGGC TGAGGACCTG CAGGGCTGGC TGGCAAGCCA GAAGCAGGCA
    GCCAAAGGAG GGGAGAGCCT GGGAGAGGAC CCCGAGCACG CCCTGCACCT CTGCACCAAG
    TTTGCAAAGT TTCAGCACCA AGTGGAGATG GGCAGCCAGC GGGTGGCCGC CTGCCGGCTG
    CTGGCGGAGA GCCTGCTAGA GCGTGGGCAC AGTGCTGGCC CCATGGTCCG TCAGAGGCAG
    CAGGATCTGC AGACCGCCTG GTCGGAGCTG TGGGAGCTGA CCCAGGCCCG AGGCCACGCG
    CTCCGAGACA CCGAGACCAC CCTCAGAGTT CACAGAGATC TCTTGGAAGT CCTCACCCAG
    GTCCAGGAGA AAGCCACGAG CCTCCCCAAC AATGTGGCAC GGGACCTGTG TGGGCTGGAG
    GCGCAGCTGA GAAGCCACCA GGGGCTGGAG CGAGAACTCG TGGGCACCGA GCGGCAGCTG
    CAGGAACTGC TGGAGACTGC AGGCAGGGTG CAGAAGCTGT GTCCGGGGCC TCAGGCCCAT
    GCGGTGCAGC AGAGGCAGCA AGCTGTGACG CAGGCGTGGG CAGTGCTGCA GCGACGCATG
    GAGCAGCGCA GGGCCCAGCT GGAGCGGGCA CGCCTCCTGG CCCGCTTCCG CACGGCGGTG
    CGTGACTATG CCTCCTGGGC AGCCCGCGTG CGCCAGGACC TGCAGGTGGA GGAGAGTTCG
    CAAGAGCCTA GCAGTGGCCC GCTGAAGCTC AGTGCCCACC AGTGGCTCCG GGCGGAGCTG
    GAGGCCCGGG AGAAGCTGTG GCAGCAGGCC ACCCAGCTGG GGCAGCAGGC ACTTCTTGCT
    GCAGGGACAC CCACCAAGGA GGTCCAGGAA GAGCTTCGAG CCCTGCAGGA CCAGCGGGAC
    CAGGTGTATC AGACCTGGGC ACGGAAGCAA GAGAGGCTGC AGGCCGAGCA GCAGGAGCAG
    CTCTTCCTCA GAGAGTGCGG CCGCCTGGAG GAGATCCTCG CGGCCCAGGA GGTCTCCCTG
    AAAACCAGTG CCTTGGGGAG CTCGGTGGAA GAGGTAGAGC AGTTGATTCG CAAGCACGAG
    GTCTTCCTGA AGGTTCTGAC TGCCCAGGAC AAGAAGGAGG CAGCCCTGCG TGAGCGGCTG
    AAGACGCTCC GGCGCCCCCG GGTGCGGGAC CGGCTTCCCA TCCTGCTGCA GCGCCGGATG
    AGAGTGAAGG AGCTGGCGGA GAGCCGGGGA CACGCCCTGC ATGCCTCCCT GCTGATGGCC
    AGCTTCACCC AGGCCGCAAC CCAGGCTGAG GACTGGATCC AGGCGTGGGC CCAGCAGCTG
    AAGGAGCCGG TCCCTCCTGG GGACCTGAGA GATAAGCTGA AGCCCCTGCT GAAACACCAG
    GCCTTTGAGG CTGAAGTCCA GGCCCATGAG GAGGTCATGA CCTCTGTTGC CAAGAAGGGA
    GAGGCTCTCC TGGCACAGAG TCACCCTCGA GCCGGAGAGG TCTCCCAGCG GCTGCAGGGC
    CTGCGGAAGC ACTGGGAGGA CCTGAGGCAG GCAATGGCCC TCAGGGGCCA GGAGCTGGAG
    GACAGGCGGA ACTTCCTGGA GTTCCTGCAG AGAGTGGACC TTGCAGAGGC CTGGATCCAG
    GAGAAGGAGG TGAAGATGAA TGTTGGTGAC CTGGGCCAGG ACCTGGAGCA CTGCCTGCAG
    CTCCGACGGC GGCTCCGCGA GTTCCGAGGA AACTCGGCCG GGGACACAGT GGGTGATGCC
    TGCATCAGGA GCATCAGTGA CTTGTCACTG CAGCTCAAGA ACCGGGACCC TGAGGAAGTC
    AAGATCATCT GCCAGCGGCG AAGCCAGCTC AACAACAGGT GGGCGAGTTT CCATGGCAAC
    TTGCTCCGGT ACCAGCAGCA GCTCGAAGGG GCCTTGGAGA TACACGTGTT GTCCCGAGAG
    CTGGACAATG TCACCAAGAG GATTCAGGAG AAGGAAGCCC TGATCCAGGC CCTGGACTGT
    GGGAAAGATC TGGAGAGCGT GCAGAGGCTG CTGCGGAAAC ACGAGGAGCT GGAGCGGGAA
    GTGCACCCCA TCCAGGCCCA GGTGGAGTCC CTAGAGCGTG AAGTGGGCCG CCTCTGCCAA
    AGAAGCCCCG AGGCAGCCCA CGGCCTCAGG CACAGGCAGC AGGAGGTGGC TGAGAGCTGG
    TGGCAGCTCC GGAGCAGGGC CCAGAAGCGG AGGGAGGCGC TGGATGCCTT GCACCAAGCT
    CAGAAACTCC AGGCAATGCT GCAGGAATTG CTGGTCAGCG CCCAGAGGCT GCGGGCTCAG
    ATGGACACGA GCCCCGCTCC TCGCAGCCCT GTGGAAGCCC GGCGTATGTT AGAAGAGCAT
    CAGGAGTGCA AGGCCGAGCT GGACTCCTGG ACAGACAGCA TCAGCCTGGC CCGAAGCACT
    GGGCAGCAAC TGCTCACAGC GGGGCACCCC TTCAGCTCCG ACATTCGCCA GGTGCTGGCT
    GGCTTAGAAC AGGAGCTGAG CAGCCTGGAA GGGGCCTGGC AGGAGCATCA GCTACAGCTG
    CAGCAGGCCC TGGAGCTACA GCTGTTTCTG AGCTCAGTGG AGAAGATGGA ACGTTGGCTT
    TGCAGCAAGG AAGACTCCCT AGCCAGTGAG GGTCTATGGG ACCCCTTGGC CCCCATGGAG
    CCCCTTCTGT GGAAGCACAA GATGCTGGAG TGGGACCTGG AGGTGCAGGC GGGAAAGATC
    AGTGCTCTGG AGGCCACGGC CCGCGGCCTG CACCAGGGTG GGCACCCCGA GGCCCAGAGT
    GCCCTGGGCA GGTGCCAGGC CATGCTTCTG AGGAAGGAGG CGCTCTTCAG GCAAGCCGGG
    ACCCGCCGCC ATCGCCTGGA GGAGCTCCGG CAGCTGCAGG CCTTCCTGCA GGACTCCCAG
    GAGGTGGCTG CGTGGCTGAG GGAGAAGAAC CTGGTGGCCT TGGAGGAGGG TTTGCTGGAC
    ACAGCCATGC TGCCAGCACA GTTACAGAAG CAGCAGAATT TCCAGGCGGA GCTGGACGCG
    AGCATGCACC AACAGCAGGA GCTGCAGCGG GAGGGACAGA GGCTGCTGCA GGGGGGCCAC
    CCAGCCTCGG AGGCCATCCA GGAGCGACTG GAGGAGCTGG GAGCACTCTG GGGTGAGCTG
    CAAGACAACT CCCAGAAGAA GGTGGCCAAG CTCCAGAAGG CCTGTGAGGC CCTGCGTCTG
    CGGCGGAGCA TGGAGGAACT GGAGAACTGG CTGGAGCCCA TCGAGGTTGA GCTGAGAGCC
    CCCACTGTGG GCCAGGCCCT GCCTGGGGTG GGCGAGCTCC TGGGCACACA GAGGGAGCTG
    GAGGCAGCAG TGGACAAGAA GGCCAGGCAG GCTGAGGCAC TGCTGGGCCA GGCCCAGGCC
    TTTGTGAGGG AAGGCCACTG CCTTGCCCAA GATGTGGAAG AGCAGGCCCG GCGGCTGCTT
    CAGAGGTTCA AGAGCCTGAG GGAGCCCCTG CAGGAGCGCA GGACGGCCCT GGAGGCCCGG
    AGCCTCCTCT TGAAGTTCTT CAGGGACGCC GACGAGGAAA TGGCCTGGGT GCAGGAGAAG
    CTGCCTCTGG CCGCTGCCCA GGACTATGGC CAGAGCCTGA GTGCGGTGCG GCACCTGCAG
    GAGCAGCACC AGAACCTGGA GAGTGAGATG AGCAGCCACG AGGCTCTGAC CCGGGTGGTG
    CTGGGCACTG GGTACAAGCT GGTGCAGGCT GGGCACTTTG CCGCCCACGA GGTGGCCGCC
    CGGGTGCAGC AGCTGGAGAA GGCCATGGCC CACCTGCGGG CAGAGGCGGC GCGGAGGCGG
    CTTCTGCTGC AGCAGGCTCA GGAGGCCCAG CAGTTTCTGA CTGAGCTCCT GGAGGCGGGA
    TCCTGGCTGG CTGAGCGGGG CCATGTCCTG GACAGCGAGG ACATGGGCCA CAGTGCTGAA
    GCCACACAGG CCCTTCTGCG GCGGCTGGAG GCCACCAAGA GAGACCTGGA AGCGTTCAGC
    CCACGCATCG AGCGGCTGCA GCAGACAGCA GCACTCCTGG AGAGCAGGAA GAACCCAGAA
    AGGTGGGCGG AAGCCACACC ATCCGCGAAG GAGCAGAGAG AAGCCCCCTA CAGAGATGGC
    AGGAGACTGC TCCAGCCTGG AAAGGCAGGG CTGCAGTCCA GACTGTCGCT CTCTTCCCAC
    AGCCCCAAGG TGCTAGCCCA GCTGCAGGCA GTTCGGGAGG CCCACGCAGA GCTGCTGCGG
    AGGGCGGAGG CCAGGGGGCA CGGCCTGCAG GAGCAGCTGC AGCTACACCA GCTGGAGCGA
    GAGACCCTGC TCCTCGACGC CTGGCTGACC ACCAAGGCGG CCACCGCCGA GTCCCAGGAC
    TACGGGCAGG ACCTGGAGGG TGTCAAGGTG CTGGAAGAGA AGTTTGATGC TTTCAGAAAG
    GAAGTGCAGA GCCTGGGCCA GGCCAAGGTG TATGCCCTGA GGAAGTTGGC AGGCACCCTG
    GAGCGGGGTG CACCCAGGCG CTATCCCCAC ATCCAAGCCC AGAGGAGCCG CATTGAGGCT
    GCTTGGGAGA GGTTGGACCA AGCAATAAAA GCCCGCACAG AGAACTTGGC TGCAGCCCAT
    GAGGTCCACA GCTTTCAGCA GGCAGCAGCT GAGCTCCAGG GAAGGATGCA GGAGAAGACG
    GCCCTGATGA AGGGGGAGGA CGGAGGCCAC AGCCTGTCAT CTGTGCGGAC CCTGCAGCAA
    CAGCACAGGC GCCTGGAGAG AGAGCTGGAA GCTATGGAGA AGGAGGTGGC ACGGCTACAG
    ACGGAGGCCT GCCGACTGGG CCAGCTACAT CCTGCAGCTC CGGGGGGCCT GGCCAAGGTG
    CAGGAGGCCT GGGCCACCCT GCAGGCGAAG GCCCAGGAGC GAGGCCAGTG GCTGGCGCAG
    GCTGCACAGG GCCATGCCTT CCTCGGGCGC TGCCAGGAAC TGCTAGCATG GGCACAGGAG
    AGGCAGGAGC TGGCGTCCTC CGAGGAGCTG GCTGAGGACG TGGCGGGGGC TGAGCAGCTC
    CTTGGGCAGC ATGAAGAGCT GGGGCAAGAA ATCAGGGAGT GCCGCCTTCA AGCCCAGGAC
    CTGCGGCAGG AAGGACAGCA GCTGGTGGAC AACAGCCACT TCATGTCTGC GGAGGTGACA
    GAGTGCCTGC AGGAGCTGGA AGGGCGGCTG CAGGAGCTGG AGGAGGCTTG GGCCCTGCGC
    TGGCAACGCT GTGCCGAGAG CTGGGGCCTG CAGAAGCTTC GGCAGAGGCT GGAGCAGGCT
    GAGGCCTGGC TGGCCTGCTG GGAGGGACTC CTGCTGAAGC CCGACTATGG GCACTCAGTG
    TCAGATGTGG AGTTGCTGCT GCACAGACAC CAGGACTTAG AAAAGCTGCT GGCAGCCCAG
    GAAGAGAAGT TTGCCCAAAT GCAAAAGACA GAGATGGAAC AGGAGCTCCT GCTGCAGCCA
    CAGGAGCTGA AGCCCGGGAG AGCTGGCAGC TCGCTGACAT CCTTTCAGTG GAGGCCCTCT
    GGACACCAGG GGCTAGGAGC ACAGCTGGCT GAGACGAGGG ACCCCCAGGA TGCAAAGGGT
    ACCCCCACCA TGGAGGGGTC TTTGGAGTTC AAGCAGCACC TGCTGCCTGG CGGGAGGCAG
    CCTAGCTCGA GCTCCTGGGA CAGCTGCCGC GGGAACTTGC AGGGCAGCTC TCTGAGCCTG
    TTCCTGGATG AGAGGATGGC AGCGGAGAAA GTAGCTTCCA TAGCCCTCCT TGACCTCACG
    GGAGCCCGGT GTGAGAGGCT GCGGGGCCGC CACGGCAGGA AACACACATT CTCCTTAAGG
    CTGACCAGTG GGGCAGAGAT CCTGTTTGCA GCACCGTCCG AAGAGCAGGC TGAGAGCTGG
    TGGCGAGCCC TGGGCAGCAC TGCAGCCCAG AGTCTGAGCC CAAAACTCAA AGCCAAACCT
    GTCAGCTCTC TGAATGAGTG CACGACCAAG GATGCCCGGC CTGGATGTCT ACTCAGGTCT
    GATCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85488
    Clone ID Related Accession (Same CDS sequence) XM_017022302.1
    Accession Version XM_017022302.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAGAGTCT GCGGGCCCTG GCACAGCTCC GCAGGGCCCG GTTGGAGGAG GCCATGGCCC 
    TGTTCGGTTT CTGCAGTTCC TGTGGGGAGC TCCAGTTGTG GCTGGAGAAG CAGACAGTGC
    TGCTCCAAAG GGTGCAGCCC CAGGCTGACA CCCTGGAGGT CATGCAGCTC AAATATGAGG
    TGGGGGCAGC TGGAGGCCCT GAAGAGGGAG AAGGCCGTGC AGCTGGCACA CAGTGTGGAA
    GTGTGCAGTT TTCTGCAGGA GTGTGGACCC ACACAGGTCC AGCTGCGAGA CGTGCTCCTC
    CAGCTGGAGG CCCTGCAGCC AGGGAGCTCA GAGGACACCT GCCACGCCCT GCAGCTGGCC
    CAGAAGAAGA CCCTGGTGCT GGAGAGGAGG GTCCACTTCC TCCAAAGTGT GGTCGTAAAG
    GTCGAGGAGC CAGGCTACGC AGAGAGCCAG CCTCTGCAAG GACAGGTGGA GACACTGCAG
    GGGCTGCTGA AGCAAGTACA GGAACAAGTG GCCCAACGGG CCCGGCGCCA GGCTGAGACT
    CAGGCCCGGC AGAGCTTCCT GCAAGAGAGC CAGCAACTGC TACTGTGGGC AGAGAGTGTC
    CAGGCTCAGC TGCGCAGCAA GGAGGTGTCA GTGGATGTGG CCTCGGCTCA GCGGCTGCTG
    AGGGAGCACC AAGACCTGCT GGAGGAGATC CACCTGTGGC AGGAGAGGCT GCAGCAGCTG
    GACGCTCAGA GCCAGCCCAT GGCAGCCTTG GACTGCCCAG ACTCCCAAGA GGTGCCCAAC
    ACTCTGAGGG TCCTGGGGCA GCAGGGCCAG GAGCTGAAGG TTTTGTGGGA GCAGAGGCAG
    CAGTGGCTGC AAGAGGGGCT GGAGCTGCAG AAGTTTGGCC GAGAAGTGGA TGGTTTCACT
    GCCACCTGTG CCAACCACCA GGCCTGGCTG CACCTGGACA ACCTTGGGGA GGACGTGAGG
    GAGGCCCTGA GCCTGCTGCA GCAGCACCGG GAGTTTGGGC GGCTCCTGAG CACCCTGGGG
    CCTCGGGCAG AGGCTCTGCG GGCACACGGC GAGAAGCTGG TTCAGAGCCA GCACCCAGCT
    GCACACACGG TCAGAGAGCA GCTGCAGAGT ATCCAGGCAC AGTGGACCAG GCTCCAGGGG
    AGGAGTGAGC AGAGGAGGAG GCAGTTGCTG GCTTCCCTCC AGCTCCAGGA GTGGAAGCAG
    GATGTGGCAG AGCTGATGCA GTGGATGGAA GAGAAGGGGC TGATGGCTGC GCATGAGCCC
    TCCGGAGCGC GCAGAAACAT CCTGCAGACA CTCAAGCGGC ACGAAGCAGC TGAGAGCGAG
    CTACTCGCCA CCCGCAGACA CGTGGAGGCC CTGCAGCAGG TTGGGAGAGA GCTGTTGAGT
    AGGAGGCCCT GTGGCCAGGA GGACATACAG ACCAGGCTTC AAGGCCTGAG AAGCAAGTGG
    GAAGCTTTGA ACCGCAAGAT GACTGAGCGT GGGGACGAGC TCCAGCAGGC TGGACAGCAG
    GAGCAACTCC TGAGGCAGCT GCAGGATGCA AAGGAGCAGC TGGAGCAGCT CGAAGGGGCC
    CTACAGAGCT CGGAAACAGG GCAGGACCTG CGCTCCAGCC AGAGGCTGCA GAAACGGCAC
    CAACAGCTGG AGAGTGAGAG CCGGACCCTG GCTGCCAAGA TGGCTGCCCT CGCCTCCATG
    GCCCATGGCA TGGCCGCCTC CCCGGCCATC CTGGAAGAGA CCCAGAAGCA CCTCCGGAGG
    CTGGAGCTTC TGCAGGGGCA TCTGGCCATC CGGGGCCTGC AGCTGCAGGC CTCAGTGGAG
    CTGCACCAGT TCTGCCACCT GAGCAACATG GAGCTCTCTT GGGTAGCCGA GCACATGCCC
    CATGGCAGCC CCACCAGCTA TACCGAGTGC TTGAATGGTG CCCAGAGCCT TCACCGCAAG
    CACAAGGAGC TCCAGGTGGA GGTAAAAGCT CACCAGGGGC AGGTGCAACG GGTGCTGAGT
    TCTGGGCGGA GCCTGGCAGC CTCAGGGCAC CCCCAAGCCC AACACATCGT GGAGCAGTGC
    CAGGAGCTGG AAGGCCACTG GGCAGAGCTG GAGAGGGCAT GTGAAGCGCG GGCCCAGTGT
    CTGCAGCAGG CTGTCACTTT CCAGCAGTAC TTTCTGGATG TGTCAGAGCT GGAGGGCTGG
    GTGGAGGAGA AGCGGCCGCT GGTGAGCAGT CGGGACTATG GCAGAGACGA GGCAGCCACC
    CTCAGGCTCA TTAACAAGCA CCAGGCTCTA CAGGAGGAAC TAGCCATTTA CTGGAGCTCC
    ATGGAGGAGC TTGACCAGAC GGCCCAAACC CTCACTGGCC CCGAAGTCCC TGAGCAGCAG
    CGTGTGGTGC AGGAGAGGCT CCGGGAGCAG CTGCGGGCAC TGCAGGAGTT GGCGGCCACA
    CGGGACCGGG AACTGGAGGG GACCCTGAGG CTGCATGAGT TCCTGAGGGA GGCTGAGGAC
    CTGCAGGGCT GGCTGGCAAG CCAGAAGCAG GCAGCCAAAG GAGGGGAGAG CCTGGGAGAG
    GACCCCGAGC ACGCCCTGCA CCTCTGCACC AAGTTTGCAA AGTTTCAGCA CCAAGTGGAG
    ATGGGCAGCC AGCGGGTGGC CGCCTGCCGG CTGCTGGCGG AGAGCCTGCT AGAGCGTGGG
    CACAGTGCTG GCCCCATGGT CCGTCAGAGG CAGCAGGATC TGCAGACCGC CTGGTCGGAG
    CTGTGGGAGC TGACCCAGGC CCGAGGCCAC GCGCTCCGAG ACACCGAGAC CACCCTCAGA
    GTTCACAGAG ATCTCTTGGA AGTCCTCACC CAGGTCCAGG AGAAAGCCAC GAGCCTCCCC
    AACAATGTGG CACGGGACCT GTGTGGGCTG GAGGCGCAGC TGAGAAGCCA CCAGGGGCTG
    GAGCGAGAAC TCGTGGGCAC CGAGCGGCAG CTGCAGGAAC TGCTGGAGAC TGCAGGCAGG
    GTGCAGAAGC TGTGTCCGGG GCCTCAGGCC CATGCGGTGC AGCAGAGGCA GCAAGCTGTG
    ACGCAGGCGT GGGCAGTGCT GCAGCGACGC ATGGAGCAGC GCAGGGCCCA GCTGGAGCGG
    GCACGCCTCC TGGCCCGCTT CCGCACGGCG GTGCGTGACT ATGCCTCCTG GGCAGCCCGC
    GTGCGCCAGG ACCTGCAGGT GGAGGAGAGT TCGCAAGAGC CTAGCAGTGG CCCGCTGAAG
    CTCAGTGCCC ACCAGTGGCT CCGGGCGGAG CTGGAGGCCC GGGAGAAGCT GTGGCAGCAG
    GCCACCCAGC TGGGGCAGCA GGCACTTCTT GCTGCAGGGA CACCCACCAA GGAGGTCCAG
    GAAGAGCTTC GAGCCCTGCA GGACCAGCGG GACCAGGTGT ATCAGACCTG GGCACGGAAG
    CAAGAGAGGC TGCAGGCCGA GCAGCAGGAG CAGCTCTTCC TCAGAGAGTG CGGCCGCCTG
    GAGGAGATCC TCGCGGCCCA GGAGGTCTCC CTGAAAACCA GTGCCTTGGG GAGCTCGGTG
    GAAGAGGTAG AGCAGTTGAT TCGCAAGCAC GAGGTCTTCC TGAAGGTTCT GACTGCCCAG
    GACAAGAAGG AGGCAGCCCT GCGTGAGCGG CTGAAGACGC TCCGGCGCCC CCGGGTGCGG
    GACCGGCTTC CCATCCTGCT GCAGCGCCGG ATGAGAGTGA AGGAGCTGGC GGAGAGCCGG
    GGACACGCCC TGCATGCCTC CCTGCTGATG GCCAGCTTCA CCCAGGCCGC AACCCAGGCT
    GAGGACTGGA TCCAGGCGTG GGCCCAGCAG CTGAAGGAGC CGGTCCCTCC TGGGGACCTG
    AGAGATAAGC TGAAGCCCCT GCTGAAACAC CAGGCCTTTG AGGCTGAAGT CCAGGCCCAT
    GAGGAGGTCA TGACCTCTGT TGCCAAGAAG GGAGAGGCTC TCCTGGCACA GAGTCACCCT
    CGAGCCGGAG AGGTCTCCCA GCGGCTGCAG GGCCTGCGGA AGCACTGGGA GGACCTGAGG
    CAGGCAATGG CCCTCAGGGG CCAGGAGCTG GAGGACAGGC GGAACTTCCT GGAGTTCCTG
    CAGAGAGTGG ACCTTGCAGA GGCCTGGATC CAGGAGAAGG AGGTGAAGAT GAATGTTGGT
    GACCTGGGCC AGGACCTGGA GCACTGCCTG CAGCTCCGAC GGCGGCTCCG CGAGTTCCGA
    GGAAACTCGG CCGGGGACAC AGTGGGTGAT GCCTGCATCA GGAGCATCAG TGACTTGTCA
    CTGCAGCTCA AGAACCGGGA CCCTGAGGAA GTCAAGATCA TCTGCCAGCG GCGAAGCCAG
    CTCAACAACA GGTGGGCGAG TTTCCATGGC AACTTGCTCC GGTACCAGCA GCAGCTCGAA
    GGGGCCTTGG AGATACACGT GTTGTCCCGA GAGCTGGACA ATGTCACCAA GAGGATTCAG
    GAGAAGGAAG CCCTGATCCA GGCCCTGGAC TGTGGGAAAG ATCTGGAGAG CGTGCAGAGG
    CTGCTGCGGA AACACGAGGA GCTGGAGCGG GAAGTGCACC CCATCCAGGC CCAGGTGGAG
    TCCCTAGAGC GTGAAGTGGG CCGCCTCTGC CAAAGAAGCC CCGAGGCAGC CCACGGCCTC
    AGGCACAGGC AGCAGGAGGT GGCTGAGAGC TGGTGGCAGC TCCGGAGCAG GGCCCAGAAG
    CGGAGGGAGG CGCTGGATGC CTTGCACCAA GCTCAGAAAC TCCAGGCAAT GCTGCAGGAA
    TTGCTGGTCA GCGCCCAGAG GCTGCGGGCT CAGATGGACA CGAGCCCCGC TCCTCGCAGC
    CCTGTGGAAG CCCGGCGTAT GTTAGAAGAG CATCAGGAGT GCAAGGCCGA GCTGGACTCC
    TGGACAGACA GCATCAGCCT GGCCCGAAGC ACTGGGCAGC AACTGCTCAC AGCGGGGCAC
    CCCTTCAGCT CCGACATTCG CCAGGTGCTG GCTGGCTTAG AACAGGAGCT GAGCAGCCTG
    GAAGGGGCCT GGCAGGAGCA TCAGCTACAG CTGCAGCAGG CCCTGGAGCT ACAGCTGTTT
    CTGAGCTCAG TGGAGAAGAT GGAACGTTGG CTTTGCAGCA AGGAAGACTC CCTAGCCAGT
    GAGGGTCTAT GGGACCCCTT GGCCCCCATG GAGCCCCTTC TGTGGAAGCA CAAGATGCTG
    GAGTGGGACC TGGAGGTGCA GGCGGGAAAG ATCAGTGCTC TGGAGGCCAC GGCCCGCGGC
    CTGCACCAGG GTGGGCACCC CGAGGCCCAG AGTGCCCTGG GCAGGTGCCA GGCCATGCTT
    CTGAGGAAGG AGGCGCTCTT CAGGCAAGCC GGGACCCGCC GCCATCGCCT GGAGGAGCTC
    CGGCAGCTGC AGGCCTTCCT GCAGGACTCC CAGGAGGTGG CTGCGTGGCT GAGGGAGAAG
    AACCTGGTGG CCTTGGAGGA GGGTTTGCTG GACACAGCCA TGCTGCCAGC ACAGTTACAG
    AAGCAGCAGA ATTTCCAGGC GGAGCTGGAC GCGAGCATGC ACCAACAGCA GGAGCTGCAG
    CGGGAGGGAC AGAGGCTGCT GCAGGGGGGC CACCCAGCCT CGGAGGCCAT CCAGGAGCGA
    CTGGAGGAGC TGGGAGCACT CTGGGGTGAG CTGCAAGACA ACTCCCAGAA GAAGGTGGCC
    AAGCTCCAGA AGGCCTGTGA GGCCCTGCGT CTGCGGCGGA GCATGGAGGA ACTGGAGAAC
    TGGCTGGAGC CCATCGAGGT TGAGCTGAGA GCCCCCACTG TGGGCCAGGC CCTGCCTGGG
    GTGGGCGAGC TCCTGGGCAC ACAGAGGGAG CTGGAGGCAG CAGTGGACAA GAAGGCCAGG
    CAGGCTGAGG CACTGCTGGG CCAGGCCCAG GCCTTTGTGA GGGAAGGCCA CTGCCTTGCC
    CAAGATGTGG AAGAGCAGGC CCGGCGGCTG CTTCAGAGGT TCAAGAGCCT GAGGGAGCCC
    CTGCAGGAGC GCAGGACGGC CCTGGAGGCC CGGAGCCTCC TCTTGAAGTT CTTCAGGGAC
    GCCGACGAGG AAATGGCCTG GGTGCAGGAG AAGCTGCCTC TGGCCGCTGC CCAGGACTAT
    GGCCAGAGCC TGAGTGCGGT GCGGCACCTG CAGGAGCAGC ACCAGAACCT GGAGAGTGAG
    ATGAGCAGCC ACGAGGCTCT GACCCGGGTG GTGCTGGGCA CTGGGTACAA GCTGGTGCAG
    GCTGGGCACT TTGCCGCCCA CGAGGTGGCC GCCCGGGTGC AGCAGCTGGA GAAGGCCATG
    GCCCACCTGC GGGCAGAGGC GGCGCGGAGG CGGCTTCTGC TGCAGCAGGC TCAGGAGGCC
    CAGCAGTTTC TGACTGAGCT CCTGGAGGCG GGATCCTGGC TGGCTGAGCG GGGCCATGTC
    CTGGACAGCG AGGACATGGG CCACAGTGCT GAAGCCACAC AGGCCCTTCT GCGGCGGCTG
    GAGGCCACCA AGAGAGACCT GGAAGCGTTC AGCCCACGCA TCGAGCGGCT GCAGCAGACA
    GCAGCACTCC TGGAGAGCAG GAAGAACCCA GAAAGGTGGG CGGAAGCCAC ACCATCCGCG
    AAGGAGCAGA GAGAAGCCCC CTACAGAGAT GGCAGGAGAC TGCTCCAGCC TGGAAAGGCA
    GGGCTGCAGT CCAGACTGTC GCTCTCTTCC CACAGCCCCA AGGTGCTAGC CCAGCTGCAG
    GCAGTTCGGG AGGCCCACGC AGAGCTGCTG CGGAGGGCGG AGGCCAGGGG GCACGGCCTG
    CAGGAGCAGC TGCAGCTACA CCAGCTGGAG CGAGAGACCC TGCTCCTCGA CGCCTGGCTG
    ACCACCAAGG CGGCCACCGC CGAGTCCCAG GACTACGGGC AGGACCTGGA GGGTGTCAAG
    GTGCTGGAAG AGAAGTTTGA TGCTTTCAGA AAGGAAGTGC AGAGCCTGGG CCAGGCCAAG
    GTGTATGCCC TGAGGAAGTT GGCAGGCACC CTGGAGCGGG GTGCACCCAG GCGCTATCCC
    CACATCCAAG CCCAGAGGAG CCGCATTGAG GCTGCTTGGG AGAGGTTGGA CCAAGCAATA
    AAAGCCCGCA CAGAGAACTT GGCTGCAGCC CATGAGGTCC ACAGCTTTCA GCAGGCAGCA
    GCTGAGCTCC AGGGAAGGAT GCAGGAGAAG ACGGCCCTGA TGAAGGGGGA GGACGGAGGC
    CACAGCCTGT CATCTGTGCG GACCCTGCAG CAACAGCACA GGCGCCTGGA GAGAGAGCTG
    GAAGCTATGG AGAAGGAGGT GGCACGGCTA CAGACGGAGG CCTGCCGACT GGGCCAGCTA
    CATCCTGCAG CTCCGGGGGG CCTGGCCAAG GTGCAGGAGG CCTGGGCCAC CCTGCAGGCG
    AAGGCCCAGG AGCGAGGCCA GTGGCTGGCG CAGGCTGCAC AGGGCCATGC CTTCCTCGGG
    CGCTGCCAGG AACTGCTAGC ATGGGCACAG GAGAGGCAGG AGCTGGCGTC CTCCGAGGAG
    CTGGCTGAGG ACGTGGCGGG GGCTGAGCAG CTCCTTGGGC AGCATGAAGA GCTGGGGCAA
    GAAATCAGGG AGTGCCGCCT TCAAGCCCAG GACCTGCGGC AGGAAGGACA GCAGCTGGTG
    GACAACAGCC ACTTCATGTC TGCGGAGGTG ACAGAGTGCC TGCAGGAGCT GGAAGGGCGG
    CTGCAGGAGC TGGAGGAGGC TTGGGCCCTG CGCTGGCAAC GCTGTGCCGA GAGCTGGGGC
    CTGCAGAAGC TTCGGCAGAG GCTGGAGCAG GCTGAGGCCT GGCTGGCCTG CTGGGAGGGA
    CTCCTGCTGA AGCCCGACTA TGGGCACTCA GTGTCAGATG TGGAGTTGCT GCTGCACAGA
    CACCAGGACT TAGAAAAGCT GCTGGCAGCC CAGGAAGAGA AGTTTGCCCA AATGCAAAAG
    ACAGAGATGG AACAGGAGCT CCTGCTGCAG CCACAGGAGC TGAAGCCCGG GAGAGCTGGC
    AGCTCGCTGA CATCCTTTCA GTGGAGGCCC TCTGGACACC AGGGGCTAGG AGCACAGCTG
    GCTGAGACGA GGGACCCCCA GGATGCAAAG GGTACCCCCA CCATGGAGGG GTCTTTGGAG
    TTCAAGCAGC ACCTGCTGCC TGGCGGGAGG CAGCCTAGCT CGAGCTCCTG GGACAGCTGC
    CGCGGGAACT TGCAGGGCAG CTCTCTGAGC CTGTTCCTGG ATGAGAGGAT GGCAGCGGAG
    AAAGTAGCTT CCATAGCCCT CCTTGACCTC ACGGGAGCCC GGTGTGAGAG GCTGCGGGGC
    CGCCACGGCA GGAAACACAC ATTCTCCTTA AGGCTGACCA GTGGGGCAGA GATCCTGTTT
    GCAGCACCGT CCGAAGAGCA GGCTGAGAGC TGGTGGCGAG CCCTGGGCAG CACTGCAGCC
    CAGAGTCTGA GCCCAAAACT CAAAGCCAAA CCTGTCAGCT CTCTGAATGA GTGCACGACC
    AAGGATGCCC GGCCTGGATG TCTACTCAGG TCTGATCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877791.1
    CDS99..8480
    Translation

    Target ORF information:

    RefSeq Version XM_017022302.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAGAGTCT GCGGGCCCTG GCACAGCTCC GCAGGGCCCG GTTGGAGGAG GCCATGGCCC 
    TGTTCGGTTT CTGCAGTTCC TGTGGGGAGC TCCAGTTGTG GCTGGAGAAG CAGACAGTGC
    TGCTCCAAAG GGTGCAGCCC CAGGCTGACA CCCTGGAGGT CATGCAGCTC AAATATGAGG
    TGGGGGCAGC TGGAGGCCCT GAAGAGGGAG AAGGCCGTGC AGCTGGCACA CAGTGTGGAA
    GTGTGCAGTT TTCTGCAGGA GTGTGGACCC ACACAGGTCC AGCTGCGAGA CGTGCTCCTC
    CAGCTGGAGG CCCTGCAGCC AGGGAGCTCA GAGGACACCT GCCACGCCCT GCAGCTGGCC
    CAGAAGAAGA CCCTGGTGCT GGAGAGGAGG GTCCACTTCC TCCAAAGTGT GGTCGTAAAG
    GTCGAGGAGC CAGGCTACGC AGAGAGCCAG CCTCTGCAAG GACAGGTGGA GACACTGCAG
    GGGCTGCTGA AGCAAGTACA GGAACAAGTG GCCCAACGGG CCCGGCGCCA GGCTGAGACT
    CAGGCCCGGC AGAGCTTCCT GCAAGAGAGC CAGCAACTGC TACTGTGGGC AGAGAGTGTC
    CAGGCTCAGC TGCGCAGCAA GGAGGTGTCA GTGGATGTGG CCTCGGCTCA GCGGCTGCTG
    AGGGAGCACC AAGACCTGCT GGAGGAGATC CACCTGTGGC AGGAGAGGCT GCAGCAGCTG
    GACGCTCAGA GCCAGCCCAT GGCAGCCTTG GACTGCCCAG ACTCCCAAGA GGTGCCCAAC
    ACTCTGAGGG TCCTGGGGCA GCAGGGCCAG GAGCTGAAGG TTTTGTGGGA GCAGAGGCAG
    CAGTGGCTGC AAGAGGGGCT GGAGCTGCAG AAGTTTGGCC GAGAAGTGGA TGGTTTCACT
    GCCACCTGTG CCAACCACCA GGCCTGGCTG CACCTGGACA ACCTTGGGGA GGACGTGAGG
    GAGGCCCTGA GCCTGCTGCA GCAGCACCGG GAGTTTGGGC GGCTCCTGAG CACCCTGGGG
    CCTCGGGCAG AGGCTCTGCG GGCACACGGC GAGAAGCTGG TTCAGAGCCA GCACCCAGCT
    GCACACACGG TCAGAGAGCA GCTGCAGAGT ATCCAGGCAC AGTGGACCAG GCTCCAGGGG
    AGGAGTGAGC AGAGGAGGAG GCAGTTGCTG GCTTCCCTCC AGCTCCAGGA GTGGAAGCAG
    GATGTGGCAG AGCTGATGCA GTGGATGGAA GAGAAGGGGC TGATGGCTGC GCATGAGCCC
    TCCGGAGCGC GCAGAAACAT CCTGCAGACA CTCAAGCGGC ACGAAGCAGC TGAGAGCGAG
    CTACTCGCCA CCCGCAGACA CGTGGAGGCC CTGCAGCAGG TTGGGAGAGA GCTGTTGAGT
    AGGAGGCCCT GTGGCCAGGA GGACATACAG ACCAGGCTTC AAGGCCTGAG AAGCAAGTGG
    GAAGCTTTGA ACCGCAAGAT GACTGAGCGT GGGGACGAGC TCCAGCAGGC TGGACAGCAG
    GAGCAACTCC TGAGGCAGCT GCAGGATGCA AAGGAGCAGC TGGAGCAGCT CGAAGGGGCC
    CTACAGAGCT CGGAAACAGG GCAGGACCTG CGCTCCAGCC AGAGGCTGCA GAAACGGCAC
    CAACAGCTGG AGAGTGAGAG CCGGACCCTG GCTGCCAAGA TGGCTGCCCT CGCCTCCATG
    GCCCATGGCA TGGCCGCCTC CCCGGCCATC CTGGAAGAGA CCCAGAAGCA CCTCCGGAGG
    CTGGAGCTTC TGCAGGGGCA TCTGGCCATC CGGGGCCTGC AGCTGCAGGC CTCAGTGGAG
    CTGCACCAGT TCTGCCACCT GAGCAACATG GAGCTCTCTT GGGTAGCCGA GCACATGCCC
    CATGGCAGCC CCACCAGCTA TACCGAGTGC TTGAATGGTG CCCAGAGCCT TCACCGCAAG
    CACAAGGAGC TCCAGGTGGA GGTAAAAGCT CACCAGGGGC AGGTGCAACG GGTGCTGAGT
    TCTGGGCGGA GCCTGGCAGC CTCAGGGCAC CCCCAAGCCC AACACATCGT GGAGCAGTGC
    CAGGAGCTGG AAGGCCACTG GGCAGAGCTG GAGAGGGCAT GTGAAGCGCG GGCCCAGTGT
    CTGCAGCAGG CTGTCACTTT CCAGCAGTAC TTTCTGGATG TGTCAGAGCT GGAGGGCTGG
    GTGGAGGAGA AGCGGCCGCT GGTGAGCAGT CGGGACTATG GCAGAGACGA GGCAGCCACC
    CTCAGGCTCA TTAACAAGCA CCAGGCTCTA CAGGAGGAAC TAGCCATTTA CTGGAGCTCC
    ATGGAGGAGC TTGACCAGAC GGCCCAAACC CTCACTGGCC CCGAAGTCCC TGAGCAGCAG
    CGTGTGGTGC AGGAGAGGCT CCGGGAGCAG CTGCGGGCAC TGCAGGAGTT GGCGGCCACA
    CGGGACCGGG AACTGGAGGG GACCCTGAGG CTGCATGAGT TCCTGAGGGA GGCTGAGGAC
    CTGCAGGGCT GGCTGGCAAG CCAGAAGCAG GCAGCCAAAG GAGGGGAGAG CCTGGGAGAG
    GACCCCGAGC ACGCCCTGCA CCTCTGCACC AAGTTTGCAA AGTTTCAGCA CCAAGTGGAG
    ATGGGCAGCC AGCGGGTGGC CGCCTGCCGG CTGCTGGCGG AGAGCCTGCT AGAGCGTGGG
    CACAGTGCTG GCCCCATGGT CCGTCAGAGG CAGCAGGATC TGCAGACCGC CTGGTCGGAG
    CTGTGGGAGC TGACCCAGGC CCGAGGCCAC GCGCTCCGAG ACACCGAGAC CACCCTCAGA
    GTTCACAGAG ATCTCTTGGA AGTCCTCACC CAGGTCCAGG AGAAAGCCAC GAGCCTCCCC
    AACAATGTGG CACGGGACCT GTGTGGGCTG GAGGCGCAGC TGAGAAGCCA CCAGGGGCTG
    GAGCGAGAAC TCGTGGGCAC CGAGCGGCAG CTGCAGGAAC TGCTGGAGAC TGCAGGCAGG
    GTGCAGAAGC TGTGTCCGGG GCCTCAGGCC CATGCGGTGC AGCAGAGGCA GCAAGCTGTG
    ACGCAGGCGT GGGCAGTGCT GCAGCGACGC ATGGAGCAGC GCAGGGCCCA GCTGGAGCGG
    GCACGCCTCC TGGCCCGCTT CCGCACGGCG GTGCGTGACT ATGCCTCCTG GGCAGCCCGC
    GTGCGCCAGG ACCTGCAGGT GGAGGAGAGT TCGCAAGAGC CTAGCAGTGG CCCGCTGAAG
    CTCAGTGCCC ACCAGTGGCT CCGGGCGGAG CTGGAGGCCC GGGAGAAGCT GTGGCAGCAG
    GCCACCCAGC TGGGGCAGCA GGCACTTCTT GCTGCAGGGA CACCCACCAA GGAGGTCCAG
    GAAGAGCTTC GAGCCCTGCA GGACCAGCGG GACCAGGTGT ATCAGACCTG GGCACGGAAG
    CAAGAGAGGC TGCAGGCCGA GCAGCAGGAG CAGCTCTTCC TCAGAGAGTG CGGCCGCCTG
    GAGGAGATCC TCGCGGCCCA GGAGGTCTCC CTGAAAACCA GTGCCTTGGG GAGCTCGGTG
    GAAGAGGTAG AGCAGTTGAT TCGCAAGCAC GAGGTCTTCC TGAAGGTTCT GACTGCCCAG
    GACAAGAAGG AGGCAGCCCT GCGTGAGCGG CTGAAGACGC TCCGGCGCCC CCGGGTGCGG
    GACCGGCTTC CCATCCTGCT GCAGCGCCGG ATGAGAGTGA AGGAGCTGGC GGAGAGCCGG
    GGACACGCCC TGCATGCCTC CCTGCTGATG GCCAGCTTCA CCCAGGCCGC AACCCAGGCT
    GAGGACTGGA TCCAGGCGTG GGCCCAGCAG CTGAAGGAGC CGGTCCCTCC TGGGGACCTG
    AGAGATAAGC TGAAGCCCCT GCTGAAACAC CAGGCCTTTG AGGCTGAAGT CCAGGCCCAT
    GAGGAGGTCA TGACCTCTGT TGCCAAGAAG GGAGAGGCTC TCCTGGCACA GAGTCACCCT
    CGAGCCGGAG AGGTCTCCCA GCGGCTGCAG GGCCTGCGGA AGCACTGGGA GGACCTGAGG
    CAGGCAATGG CCCTCAGGGG CCAGGAGCTG GAGGACAGGC GGAACTTCCT GGAGTTCCTG
    CAGAGAGTGG ACCTTGCAGA GGCCTGGATC CAGGAGAAGG AGGTGAAGAT GAATGTTGGT
    GACCTGGGCC AGGACCTGGA GCACTGCCTG CAGCTCCGAC GGCGGCTCCG CGAGTTCCGA
    GGAAACTCGG CCGGGGACAC AGTGGGTGAT GCCTGCATCA GGAGCATCAG TGACTTGTCA
    CTGCAGCTCA AGAACCGGGA CCCTGAGGAA GTCAAGATCA TCTGCCAGCG GCGAAGCCAG
    CTCAACAACA GGTGGGCGAG TTTCCATGGC AACTTGCTCC GGTACCAGCA GCAGCTCGAA
    GGGGCCTTGG AGATACACGT GTTGTCCCGA GAGCTGGACA ATGTCACCAA GAGGATTCAG
    GAGAAGGAAG CCCTGATCCA GGCCCTGGAC TGTGGGAAAG ATCTGGAGAG CGTGCAGAGG
    CTGCTGCGGA AACACGAGGA GCTGGAGCGG GAAGTGCACC CCATCCAGGC CCAGGTGGAG
    TCCCTAGAGC GTGAAGTGGG CCGCCTCTGC CAAAGAAGCC CCGAGGCAGC CCACGGCCTC
    AGGCACAGGC AGCAGGAGGT GGCTGAGAGC TGGTGGCAGC TCCGGAGCAG GGCCCAGAAG
    CGGAGGGAGG CGCTGGATGC CTTGCACCAA GCTCAGAAAC TCCAGGCAAT GCTGCAGGAA
    TTGCTGGTCA GCGCCCAGAG GCTGCGGGCT CAGATGGACA CGAGCCCCGC TCCTCGCAGC
    CCTGTGGAAG CCCGGCGTAT GTTAGAAGAG CATCAGGAGT GCAAGGCCGA GCTGGACTCC
    TGGACAGACA GCATCAGCCT GGCCCGAAGC ACTGGGCAGC AACTGCTCAC AGCGGGGCAC
    CCCTTCAGCT CCGACATTCG CCAGGTGCTG GCTGGCTTAG AACAGGAGCT GAGCAGCCTG
    GAAGGGGCCT GGCAGGAGCA TCAGCTACAG CTGCAGCAGG CCCTGGAGCT ACAGCTGTTT
    CTGAGCTCAG TGGAGAAGAT GGAACGTTGG CTTTGCAGCA AGGAAGACTC CCTAGCCAGT
    GAGGGTCTAT GGGACCCCTT GGCCCCCATG GAGCCCCTTC TGTGGAAGCA CAAGATGCTG
    GAGTGGGACC TGGAGGTGCA GGCGGGAAAG ATCAGTGCTC TGGAGGCCAC GGCCCGCGGC
    CTGCACCAGG GTGGGCACCC CGAGGCCCAG AGTGCCCTGG GCAGGTGCCA GGCCATGCTT
    CTGAGGAAGG AGGCGCTCTT CAGGCAAGCC GGGACCCGCC GCCATCGCCT GGAGGAGCTC
    CGGCAGCTGC AGGCCTTCCT GCAGGACTCC CAGGAGGTGG CTGCGTGGCT GAGGGAGAAG
    AACCTGGTGG CCTTGGAGGA GGGTTTGCTG GACACAGCCA TGCTGCCAGC ACAGTTACAG
    AAGCAGCAGA ATTTCCAGGC GGAGCTGGAC GCGAGCATGC ACCAACAGCA GGAGCTGCAG
    CGGGAGGGAC AGAGGCTGCT GCAGGGGGGC CACCCAGCCT CGGAGGCCAT CCAGGAGCGA
    CTGGAGGAGC TGGGAGCACT CTGGGGTGAG CTGCAAGACA ACTCCCAGAA GAAGGTGGCC
    AAGCTCCAGA AGGCCTGTGA GGCCCTGCGT CTGCGGCGGA GCATGGAGGA ACTGGAGAAC
    TGGCTGGAGC CCATCGAGGT TGAGCTGAGA GCCCCCACTG TGGGCCAGGC CCTGCCTGGG
    GTGGGCGAGC TCCTGGGCAC ACAGAGGGAG CTGGAGGCAG CAGTGGACAA GAAGGCCAGG
    CAGGCTGAGG CACTGCTGGG CCAGGCCCAG GCCTTTGTGA GGGAAGGCCA CTGCCTTGCC
    CAAGATGTGG AAGAGCAGGC CCGGCGGCTG CTTCAGAGGT TCAAGAGCCT GAGGGAGCCC
    CTGCAGGAGC GCAGGACGGC CCTGGAGGCC CGGAGCCTCC TCTTGAAGTT CTTCAGGGAC
    GCCGACGAGG AAATGGCCTG GGTGCAGGAG AAGCTGCCTC TGGCCGCTGC CCAGGACTAT
    GGCCAGAGCC TGAGTGCGGT GCGGCACCTG CAGGAGCAGC ACCAGAACCT GGAGAGTGAG
    ATGAGCAGCC ACGAGGCTCT GACCCGGGTG GTGCTGGGCA CTGGGTACAA GCTGGTGCAG
    GCTGGGCACT TTGCCGCCCA CGAGGTGGCC GCCCGGGTGC AGCAGCTGGA GAAGGCCATG
    GCCCACCTGC GGGCAGAGGC GGCGCGGAGG CGGCTTCTGC TGCAGCAGGC TCAGGAGGCC
    CAGCAGTTTC TGACTGAGCT CCTGGAGGCG GGATCCTGGC TGGCTGAGCG GGGCCATGTC
    CTGGACAGCG AGGACATGGG CCACAGTGCT GAAGCCACAC AGGCCCTTCT GCGGCGGCTG
    GAGGCCACCA AGAGAGACCT GGAAGCGTTC AGCCCACGCA TCGAGCGGCT GCAGCAGACA
    GCAGCACTCC TGGAGAGCAG GAAGAACCCA GAAAGGTGGG CGGAAGCCAC ACCATCCGCG
    AAGGAGCAGA GAGAAGCCCC CTACAGAGAT GGCAGGAGAC TGCTCCAGCC TGGAAAGGCA
    GGGCTGCAGT CCAGACTGTC GCTCTCTTCC CACAGCCCCA AGGTGCTAGC CCAGCTGCAG
    GCAGTTCGGG AGGCCCACGC AGAGCTGCTG CGGAGGGCGG AGGCCAGGGG GCACGGCCTG
    CAGGAGCAGC TGCAGCTACA CCAGCTGGAG CGAGAGACCC TGCTCCTCGA CGCCTGGCTG
    ACCACCAAGG CGGCCACCGC CGAGTCCCAG GACTACGGGC AGGACCTGGA GGGTGTCAAG
    GTGCTGGAAG AGAAGTTTGA TGCTTTCAGA AAGGAAGTGC AGAGCCTGGG CCAGGCCAAG
    GTGTATGCCC TGAGGAAGTT GGCAGGCACC CTGGAGCGGG GTGCACCCAG GCGCTATCCC
    CACATCCAAG CCCAGAGGAG CCGCATTGAG GCTGCTTGGG AGAGGTTGGA CCAAGCAATA
    AAAGCCCGCA CAGAGAACTT GGCTGCAGCC CATGAGGTCC ACAGCTTTCA GCAGGCAGCA
    GCTGAGCTCC AGGGAAGGAT GCAGGAGAAG ACGGCCCTGA TGAAGGGGGA GGACGGAGGC
    CACAGCCTGT CATCTGTGCG GACCCTGCAG CAACAGCACA GGCGCCTGGA GAGAGAGCTG
    GAAGCTATGG AGAAGGAGGT GGCACGGCTA CAGACGGAGG CCTGCCGACT GGGCCAGCTA
    CATCCTGCAG CTCCGGGGGG CCTGGCCAAG GTGCAGGAGG CCTGGGCCAC CCTGCAGGCG
    AAGGCCCAGG AGCGAGGCCA GTGGCTGGCG CAGGCTGCAC AGGGCCATGC CTTCCTCGGG
    CGCTGCCAGG AACTGCTAGC ATGGGCACAG GAGAGGCAGG AGCTGGCGTC CTCCGAGGAG
    CTGGCTGAGG ACGTGGCGGG GGCTGAGCAG CTCCTTGGGC AGCATGAAGA GCTGGGGCAA
    GAAATCAGGG AGTGCCGCCT TCAAGCCCAG GACCTGCGGC AGGAAGGACA GCAGCTGGTG
    GACAACAGCC ACTTCATGTC TGCGGAGGTG ACAGAGTGCC TGCAGGAGCT GGAAGGGCGG
    CTGCAGGAGC TGGAGGAGGC TTGGGCCCTG CGCTGGCAAC GCTGTGCCGA GAGCTGGGGC
    CTGCAGAAGC TTCGGCAGAG GCTGGAGCAG GCTGAGGCCT GGCTGGCCTG CTGGGAGGGA
    CTCCTGCTGA AGCCCGACTA TGGGCACTCA GTGTCAGATG TGGAGTTGCT GCTGCACAGA
    CACCAGGACT TAGAAAAGCT GCTGGCAGCC CAGGAAGAGA AGTTTGCCCA AATGCAAAAG
    ACAGAGATGG AACAGGAGCT CCTGCTGCAG CCACAGGAGC TGAAGCCCGG GAGAGCTGGC
    AGCTCGCTGA CATCCTTTCA GTGGAGGCCC TCTGGACACC AGGGGCTAGG AGCACAGCTG
    GCTGAGACGA GGGACCCCCA GGATGCAAAG GGTACCCCCA CCATGGAGGG GTCTTTGGAG
    TTCAAGCAGC ACCTGCTGCC TGGCGGGAGG CAGCCTAGCT CGAGCTCCTG GGACAGCTGC
    CGCGGGAACT TGCAGGGCAG CTCTCTGAGC CTGTTCCTGG ATGAGAGGAT GGCAGCGGAG
    AAAGTAGCTT CCATAGCCCT CCTTGACCTC ACGGGAGCCC GGTGTGAGAG GCTGCGGGGC
    CGCCACGGCA GGAAACACAC ATTCTCCTTA AGGCTGACCA GTGGGGCAGA GATCCTGTTT
    GCAGCACCGT CCGAAGAGCA GGCTGAGAGC TGGTGGCGAG CCCTGGGCAG CACTGCAGCC
    CAGAGTCTGA GCCCAAAACT CAAAGCCAAA CCTGTCAGCT CTCTGAATGA GTGCACGACC
    AAGGATGCCC GGCCTGGATG TCTACTCAGG TCTGATCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85489
    Clone ID Related Accession (Same CDS sequence) XM_017022303.1
    Accession Version XM_017022303.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGGAGGGAG GCGCTGGATG CCTTGCACCA AGCTCAGAAA CTCCAGGCAA TGCTGCAGGA
    ATTGCTGGTC AGCGCCCAGA GGCTGCGGGC TCAGATGGAC ACGAGCCCCG CTCCTCGCAG
    CCCTGTGGAA GCCCGGCGTA TGTTAGAAGA GCATCAGGAG TGCAAGGCCG AGCTGGACTC
    CTGGACAGAC AGCATCAGCC TGGCCCGAAG CACTGGGCAG CAACTGCTCA CAGCGGGGCA
    CCCCTTCAGC TCCGACATTC GCCAGGTGCT GGCTGGCTTA GAACAGGAGC TGAGCAGCCT
    GGAAGGGGCC TGGCAGGAGC ATCAGCTACA GCTGCAGCAG GCCCTGGAGC TACAGCTGTT
    TCTGAGCTCA GTGGAGAAGA TGGAACGTTG GCTTTGCAGC AAGGAAGACT CCCTAGCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877792.1
    CDS27..7769
    Translation

    Target ORF information:

    RefSeq Version XM_017022303.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGGA CACAGTGGGT GATGCCTGCA TCAGGAGCAT CAGTGACTTG
    TCACTGCAGC TCAAGAACCG GGACCCTGAG GAAGTCAAGA TCATCTGCCA GCGGCGAAGC
    CAGCTCAACA ACAGGTGGGC GAGTTTCCAT GGCAACTTGC TCCGGTACCA GCAGCAGCTC
    GAAGGGGCCT TGGAGATACA CGTGTTGTCC CGAGAGCTGG ACAATGTCAC CAAGAGGATT
    CAGGAGAAGG AAGCCCTGAT CCAGGCCCTG GACTGTGGGA AAGATCTGGA GAGCGTGCAG
    AGGCTGCTGC GGAAACACGA GGAGCTGGAG CGGGAAGTGC ACCCCATCCA GGCCCAGGTG
    GAGGAGGGAG GCGCTGGATG CCTTGCACCA AGCTCAGAAA CTCCAGGCAA TGCTGCAGGA
    ATTGCTGGTC AGCGCCCAGA GGCTGCGGGC TCAGATGGAC ACGAGCCCCG CTCCTCGCAG
    CCCTGTGGAA GCCCGGCGTA TGTTAGAAGA GCATCAGGAG TGCAAGGCCG AGCTGGACTC
    CTGGACAGAC AGCATCAGCC TGGCCCGAAG CACTGGGCAG CAACTGCTCA CAGCGGGGCA
    CCCCTTCAGC TCCGACATTC GCCAGGTGCT GGCTGGCTTA GAACAGGAGC TGAGCAGCCT
    GGAAGGGGCC TGGCAGGAGC ATCAGCTACA GCTGCAGCAG GCCCTGGAGC TACAGCTGTT
    TCTGAGCTCA GTGGAGAAGA TGGAACGTTG GCTTTGCAGC AAGGAAGACT CCCTAGCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85490
    Clone ID Related Accession (Same CDS sequence) XM_017022304.1
    Accession Version XM_017022304.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGAA TGGGGGTAGC ACCCACCTCA GAGGTAAGAG AGCACGTGTG
    TGGTGGCAGG TACGTGAATC TCTAGCTGTG ATAAAACTGC ATAGACCTAT CCCCCACTGC
    CCCGCACAGA TGCATACTTG CAAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877793.1
    CDS27..7136
    Translation

    Target ORF information:

    RefSeq Version XM_017022304.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022304.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAC CGCCTGGTCG
    GAGCTGTGGG AGCTGACCCA GGCCCGAGGC CACGCGCTCC GAGACACCGA GACCACCCTC
    AGAGTTCACA GAGATCTCTT GGAAGTCCTC ACCCAGGTCC AGGAGAAAGC CACGAGCCTC
    CCCAACAATG TGGCACGGGA CCTGTGTGGG CTGGAGGCGC AGCTGAGAAG CCACCAGGGG
    CTGGAGCGAG AACTCGTGGG CACCGAGCGG CAGCTGCAGG AACTGCTGGA GACTGCAGGC
    AGGGTGCAGA AGCTGTGTCC GGGGCCTCAG GCCCATGCGG TGCAGCAGAG GCAGCAAGCT
    GTGACGCAGG CGTGGGCAGT GCTGCAGCGA CGCATGGAGC AGCGCAGGGC CCAGCTGGAG
    CGGGCACGCC TCCTGGCCCG CTTCCGCACG GCGGTGCGTG ACTATGCCTC CTGGGCAGCC
    CGCGTGCGCC AGGACCTGCA GGTGGAGGAG AGTTCGCAAG AGCCTAGCAG TGGCCCGCTG
    AAGCTCAGTG CCCACCAGTG GCTCCGGGCG GAGCTGGAGG CCCGGGAGAA GCTGTGGCAG
    CAGGCCACCC AGCTGGGGCA GCAGGCACTT CTTGCTGCAG GGACACCCAC CAAGGAGGTC
    CAGGAAGAGC TTCGAGCCCT GCAGGACCAG CGGGACCAGG TGTATCAGAC CTGGGCACGG
    AAGCAAGAGA GGCTGCAGGC CGAGCAGCAG GAGCAGCTCT TCCTCAGAGA GTGCGGCCGC
    CTGGAGGAGA TCCTCGCGGC CCAGGAGGTC TCCCTGAAAA CCAGTGCCTT GGGGAGCTCG
    GTGGAAGAGG TAGAGCAGTT GATTCGCAAG CACGAGGTCT TCCTGAAGGT TCTGACTGCC
    CAGGACAAGA AGGAGGCAGC CCTGCGTGAG CGGCTGAAGA CGCTCCGGCG CCCCCGGGTG
    CGGGACCGGC TTCCCATCCT GCTGCAGCGC CGGATGAGAG TGAAGGAGCT GGCGGAGAGC
    CGGGGACACG CCCTGCATGC CTCCCTGCTG ATGGCCAGCT TCACCCAGGC CGCAACCCAG
    GCTGAGGACT GGATCCAGGC GTGGGCCCAG CAGCTGAAGG AGCCGGTCCC TCCTGGGGAC
    CTGAGAGATA AGCTGAAGCC CCTGCTGAAA CACCAGGCCT TTGAGGCTGA AGTCCAGGCC
    CATGAGGAGG TCATGACCTC TGTTGCCAAG AAGGGAGAGG CTCTCCTGGC ACAGAGTCAC
    CCTCGAGCCG GAGAGGTCTC CCAGCGGCTG CAGGGCCTGC GGAAGCACTG GGAGGACCTG
    AGGCAGGCAA TGGCCCTCAG GGGCCAGGAG CTGGAGGACA GGCGGAACTT CCTGGAGTTC
    CTGCAGAGAG TGGACCTTGC AGAGGCCTGG ATCCAGGAGA AGGAGGTGAA GATGAATGTT
    GGTGACCTGG GCCAGGACCT GGAGCACTGC CTGCAGCTCC GACGGCGGCT CCGCGAGTTC
    CGAGGAAACT CGGCCGGGAA TGGGGGTAGC ACCCACCTCA GAGGTAAGAG AGCACGTGTG
    TGGTGGCAGG TACGTGAATC TCTAGCTGTG ATAAAACTGC ATAGACCTAT CCCCCACTGC
    CCCGCACAGA TGCATACTTG CAAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85491
    Clone ID Related Accession (Same CDS sequence) XM_017022305.1
    Accession Version XM_017022305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAG AAAGCCACGA
    GCCTCCCCAA CAATGTGGCA CGGGACCTGT GTGGGCTGGA GGCGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877794.1
    CDS27..5597
    Translation

    Target ORF information:

    RefSeq Version XM_017022305.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCCTCCA GGTTTAGGGA GCCATCCAGC AGCTCCTCAT CCCCAAAGTC TGCAGGGCTG 
    ATGGCTGGTC AGCCCCACAG TCCCCGGGAG CTCCTCGGGG CTGCAGGGCA CCGCAGCAGG
    AGGCCCAGCA CAGAACTCCG GGTCCCGCCC AGTCCAAGTC TCACCATGGA CTCTCAGTAC
    GAGACGGGCC ACATTCGCAA GCTACAGGCC CGGCACATGC AGATGCAGGA GAAGACTTTC
    ACCAAGTGGA TCAATAACGT CTTCCAGTGC GGCCAGGCGG GCATCAAGAT CCGGAACCTG
    TACACAGAGC TGGCTGACGG CATCCACCTC CTGCGGCTGC TGGAGCTCAT CTCAGGGGAG
    GCCCTGCCAC CCCCGAGCCG GGGCCGCCTG CGTGTGCACT TCCTGGAGAA CAGCAGCCGA
    GCTCTGGCCT TCCTCAGGGC CAAGGTGCCA GTACCACTCA TCGGGCCAGA GAACATCGTG
    GACGGAGACC AGACACTCAT CCTGGGACTC ATCTGGGTCA TCATTCTGCG TTTCCAGATC
    TCCCACATCT CCTTGGACAA GGAGGAGTTT GGGGCCAGCG CAGCCCTGCT GTCCACCAAG
    GAAGCCCTGC TGGTCTGGTG CCAGCGGAAG ACAGCCAGCT ACACCAACGT GAACATTACA
    GATTTCTCCC GAAGCTGGAG CGATGGGCTG GGCTTCAATG CCCTCATCCA TGCCCACAGG
    CCAGACCTGT TGGACTACGG CTCCCTGCGT CCAGACCGCC CACTGCACAA CCTTGCTTTT
    GCTTTCCTGG TGGCTGAGCA GGAGCTGGGC ATTGCTCAGC TGCTGGACCC CGAGGACGTG
    GCAGCCGCAC AGCCAGATGA GCGCTCTATC ATGACCTACG TCTCCCTCTA CTACCACTAC
    TGCTCCCGCC TGCATCAGGG GCAGACTGTC CAGAGGAGAC TCACTAAGAT CCTGCTTCAG
    CTCCAGGAGA CAGAGCTGCT GCAGACCCAG TACGAGCAGC TGGTGGCTGA CCTTCTACGC
    TGGATTGCAG AGAAGCAGAT GCAGCTGGAG GCGCGGGATT TTCCAGACTC GCTGCCCGCC
    ATGCGGCAGC TACTGGCAGC ATTCACCATC TTCCGCACCC AGGAGAAGCC ACCCCGGCTA
    CAGCAGCGAG GGGCCGCAGA GGCCCTGCTC TTCCGGCTAC AGACAGCACT CCAAGCCCAG
    AACCGCAGGC CCTTCCTGCC TCATGAGGGC CTGGGCCTTG CAGAGCTGTC CCAGTGCTGG
    GCAGGGCTGG AGTGGGCAGA GGCTGCAAGG AGCCAGGCCC TGCAGCAGAG GCTACTGCAG
    CTGCAGCGGC TAGAAACCCT GGCCCGGCGC TTCCAGCACA AGGCAGCCCT CCGGGAGAGT
    TTCCTTAAGG ATGCAGAGCA GGTGCTAGAC CAGGCCAGAG CCCCGCCAGC CAGCCTGGCC
    ACAGTGGAGG CAGCCGTCCA GAGGCTGGGC ATGCTGGAGG CTGGCATCCT GCCCCAGGAG
    GGGCGCTTCC AGGCCCTGGC TGAGATCGCA GACATCCTCC GGCAGGAGCA GTACCACAGC
    TGGGCAGATG TGGCCCGCAG GCAGGAGGAA GTTACCGTGC GCTGGCAGAG GCTCCTTCAG
    CATCTACAGG GACAGAGGAA GCAGGTGGCA GACATGCAGG CTGTGCTGAG CCTGCTGCAG
    GAGGTGGAGG CTGCCTCCCA CCAGCTGGAG GAGCTGCAGG AGCCGGCCAG GTCCACCGCC
    TGTGGGCAGC AGCTGGCAGA AGTGGTGGAG CTGCTGCAGA GGCATGACCT GCTGGAGGCT
    CAAGTCTCGG CCCACGGAGC CCATGTGAGC CATCTTGCTC AGCAGACAGC AGAGCTGGAC
    TCCTCCCTGG GCACCAGTGT GGAGGTGCTG CAGGCCAAGG CCAGGACACT GGCCCAGCTC
    CAACAGAGCC TGGTGGCTCT TGTCAGGGCC CGGCGGGCCC TGCTGGAGCA GACCCTGCAG
    CGGGCAGAGT TCCTGCGCAA CTGTGAGGAG GAGGAAGCCT GGCTGAAGGA GTGCGGACAG
    CGGGTGGGGA ATGCGGCCCT GGGCCGGGAT CTCAGCCAGA TCGCAGGCGC CCTGCAGAAA
    CACAAGGCCC TGGAAGCTGA GGTCCACCGC CACCAGGCCG TGTGCGTAGA TCTCGTGCGG
    AGGGGACGCG ACCTCAGCGC CCGCAGGCCC CCAACGCAGC CGGATCCCGG GGAACGGGCA
    GAGGCCGTTC AGGGAGGGTG GCAGCTGCTC CAGACCCGGG TGGTGGGGCG GGGCGCACGG
    CTGCAGACAG CCCTGCTGGT CCTGCAGTAC TTCGCGGACG CGGCGGAGGC GGCTTCGTGG
    CTGCGCGAGC GGCGATCCTC GCTGGAGAGA GCGTCCTGCG GTCAGGACCA GGCGGCCGCC
    GAGACCCTGC TGAGGCGCCA CGTGCGGCTG GAGCGCGTCC TGCGCGCCTT CGCGGCCGAG
    CTGCGGCGGC TGGAGGAGCA GGGGCGGGCG GCCTCGGCCC GGGCGTCGTT ATTCACGGTG
    AACTCTGCCC TGAGCCCTCC AGGAGAAAGC CTGAGGAACC CAGGGCCCTG GAGTGAGGCT
    TCCTGCCACC CTGGCCCTGG GGATGCCTGG AAGATGGCCC TCCCAGCTGA GCCTGACCCT
    GACTTTGATC CCAACACTAT ACTCCAGACA CAGGACCACT TGAGTCAGGA CTATGAGAGT
    CTGCGGGCCC TGGCACAGCT CCGCAGGGCC CGGTTGGAGG AGGCCATGGC CCTGTTCGGT
    TTCTGCAGTT CCTGTGGGGA GCTCCAGTTG TGGCTGGAGA AGCAGACAGT GCTGCTCCAA
    AGGGTGCAGC CCCAGGCTGA CACCCTGGAG GTCATGCAGC TCAAATATGA GAACTTCCTC
    ACTGCCCTGG CTGTGGGAAA GGGCCTCTGG GCTGAGGTCA GCAGCTCTGC TGAGCAACTG
    AGGCAGAGAT ATCCTGGGAA CTCCACACAA ATCCAACGAC AGCAGGAGGA ACTGAGCCAG
    AGGTGGGGGC AGCTGGAGGC CCTGAAGAGG GAGAAGGCCG TGCAGCTGGC ACACAGTGTG
    GAAGTGTGCA GTTTTCTGCA GGAGTGTGGA CCCACACAGG TCCAGCTGCG AGACGTGCTC
    CTCCAGCTGG AGGCCCTGCA GCCAGGGAGC TCAGAGGACA CCTGCCACGC CCTGCAGCTG
    GCCCAGAAGA AGACCCTGGT GCTGGAGAGG AGGGTCCACT TCCTCCAAAG TGTGGTCGTA
    AAGGTCGAGG AGCCAGGCTA CGCAGAGAGC CAGCCTCTGC AAGGACAGGT GGAGACACTG
    CAGGGGCTGC TGAAGCAAGT ACAGGAACAA GTGGCCCAAC GGGCCCGGCG CCAGGCTGAG
    ACTCAGGCCC GGCAGAGCTT CCTGCAAGAG AGCCAGCAAC TGCTACTGTG GGCAGAGAGT
    GTCCAGGCTC AGCTGCGCAG CAAGGAGGTG TCAGTGGATG TGGCCTCGGC TCAGCGGCTG
    CTGAGGGAGC ACCAAGACCT GCTGGAGGAG ATCCACCTGT GGCAGGAGAG GCTGCAGCAG
    CTGGACGCTC AGAGCCAGCC CATGGCAGCC TTGGACTGCC CAGACTCCCA AGAGGTGCCC
    AACACTCTGA GGGTCCTGGG GCAGCAGGGC CAGGAGCTGA AGGTTTTGTG GGAGCAGAGG
    CAGCAGTGGC TGCAAGAGGG GCTGGAGCTG CAGAAGTTTG GCCGAGAAGT GGATGGTTTC
    ACTGCCACCT GTGCCAACCA CCAGGCCTGG CTGCACCTGG ACAACCTTGG GGAGGACGTG
    AGGGAGGCCC TGAGCCTGCT GCAGCAGCAC CGGGAGTTTG GGCGGCTCCT GAGCACCCTG
    GGGCCTCGGG CAGAGGCTCT GCGGGCACAC GGCGAGAAGC TGGTTCAGAG CCAGCACCCA
    GCTGCACACA CGGTCAGAGA GCAGCTGCAG AGTATCCAGG CACAGTGGAC CAGGCTCCAG
    GGGAGGAGTG AGCAGAGGAG GAGGCAGTTG CTGGCTTCCC TCCAGCTCCA GGAGTGGAAG
    CAGGATGTGG CAGAGCTGAT GCAGTGGATG GAAGAGAAGG GGCTGATGGC TGCGCATGAG
    CCCTCCGGAG CGCGCAGAAA CATCCTGCAG ACACTCAAGC GGCACGAAGC AGCTGAGAGC
    GAGCTACTCG CCACCCGCAG ACACGTGGAG GCCCTGCAGC AGGTTGGGAG AGAGCTGTTG
    AGTAGGAGGC CCTGTGGCCA GGAGGACATA CAGACCAGGC TTCAAGGCCT GAGAAGCAAG
    TGGGAAGCTT TGAACCGCAA GATGACTGAG CGTGGGGACG AGCTCCAGCA GGCTGGACAG
    CAGGAGCAAC TCCTGAGGCA GCTGCAGGAT GCAAAGGAGC AGCTGGAGCA GCTCGAAGGG
    GCCCTACAGA GCTCGGAAAC AGGGCAGGAC CTGCGCTCCA GCCAGAGGCT GCAGAAACGG
    CACCAACAGC TGGAGAGTGA GAGCCGGACC CTGGCTGCCA AGATGGCTGC CCTCGCCTCC
    ATGGCCCATG GCATGGCCGC CTCCCCGGCC ATCCTGGAAG AGACCCAGAA GCACCTCCGG
    AGGCTGGAGC TTCTGCAGGG GCATCTGGCC ATCCGGGGCC TGCAGCTGCA GGCCTCAGTG
    GAGCTGCACC AGTTCTGCCA CCTGAGCAAC ATGGAGCTCT CTTGGGTAGC CGAGCACATG
    CCCCATGGCA GCCCCACCAG CTATACCGAG TGCTTGAATG GTGCCCAGAG CCTTCACCGC
    AAGCACAAGG AGCTCCAGGT GGAGGTAAAA GCTCACCAGG GGCAGGTGCA ACGGGTGCTG
    AGTTCTGGGC GGAGCCTGGC AGCCTCAGGG CACCCCCAAG CCCAACACAT CGTGGAGCAG
    TGCCAGGAGC TGGAAGGCCA CTGGGCAGAG CTGGAGAGGG CATGTGAAGC GCGGGCCCAG
    TGTCTGCAGC AGGCTGTCAC TTTCCAGCAG TACTTTCTGG ATGTGTCAGA GCTGGAGGGC
    TGGGTGGAGG AGAAGCGGCC GCTGGTGAGC AGTCGGGACT ATGGCAGAGA CGAGGCAGCC
    ACCCTCAGGC TCATTAACAA GCACCAGGCT CTACAGGAGG AACTAGCCAT TTACTGGAGC
    TCCATGGAGG AGCTTGACCA GACGGCCCAA ACCCTCACTG GCCCCGAAGT CCCTGAGCAG
    CAGCGTGTGG TGCAGGAGAG GCTCCGGGAG CAGCTGCGGG CACTGCAGGA GTTGGCGGCC
    ACACGGGACC GGGAACTGGA GGGGACCCTG AGGCTGCATG AGTTCCTGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC AAGCCAGAAG CAGGCAGCCA AAGGAGGGGA GAGCCTGGGA
    GAGGACCCCG AGCACGCCCT GCACCTCTGC ACCAAGTTTG CAAAGTTTCA GCACCAAGTG
    GAGATGGGCA GCCAGCGGGT GGCCGCCTGC CGGCTGCTGG CGGAGAGCCT GCTAGAGCGT
    GGGCACAGTG CTGGCCCCAT GGTCCGTCAG AGGCAGCAGG ATCTGCAGAG AAAGCCACGA
    GCCTCCCCAA CAATGTGGCA CGGGACCTGT GTGGGCTGGA GGCGCAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.