TLR7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR7
Entrez Gene ID	51284
Full Name	toll like receptor 7
Synonyms	TLR7-like
General protein information	Preferred Names toll like receptor 7 Names toll-like receptor 7 toll-like receptor 7-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.2
Summary	The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008].
Disorder MIM:	300365

mRNA and Protein(s)

mRNA	Protein	Name
NM_016562.4	NP_057646.1	toll-like receptor 7 precursor
NM_016562.3	NP_057646.1	toll-like receptor 7 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLR7	NP_057646.1
Pan troglodytes (chimpanzee)	TLR7	NP_001123605.1
Canis lupus familiaris (dog)	TLR7	NP_001041589.1
Danio rerio (zebrafish)	tlr7	XP_003199309.1
	TLR7	NP_001123898.1
Bos taurus (cattle)	TLR7	NP_001028933.1
Mus musculus (house mouse)	Tlr7	NP_573474.1
Gallus gallus (chicken)	TLR7	NP_001011688.1
Rattus norvegicus (Norway rat)	Tlr7	NP_001091051.1
Xenopus tropicalis (tropical clawed frog)	tlr7	NP_001120883.1

Gene Ontology

The following TLR7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19456	NM_016562.4 Latest version!	Homo sapiens toll like receptor 7 (TLR7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
View Old Accession Versions >>
OHu19456	NM_016562.3	Homo sapiens toll like receptor 7 (TLR7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR7 ( NM_016562.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057646.1 Homo sapiens toll like receptor 7 (TLR7), mRNA.

Price & Availability↑

CloneID	OHu19456
Clone ID Related Accession (Same CDS sequence)	NM_016562.4 , NM_016562.3
Accession Version	NM_016562.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	toll-like receptor 7 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005859.1, BC033651.1 and AF240467.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016562.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313858.1, AF240467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380659.4/ ENSP00000370034.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGTTTC CAATGTGGAC ACTGAAGAGA CAAATTCTTA TCCTTTTTAA CATAATCCTA 
ATTTCCAAAC TCCTTGGGGC TAGATGGTTT CCTAAAACTC TGCCCTGTGA TGTCACTCTG 
GATGTTCCAA AGAACCATGT GATCGTGGAC TGCACAGACA AGCATTTGAC AGAAATTCCT 
GGAGGTATTC CCACGAACAC CACGAACCTC ACCCTCACCA TTAACCACAT ACCAGACATC 
TCCCCAGCGT CCTTTCACAG ACTGGACCAT CTGGTAGAGA TCGATTTCAG ATGCAACTGT 
GTACCTATTC CACTGGGGTC AAAAAACAAC ATGTGCATCA AGAGGCTGCA GATTAAACCC 
AGAAGCTTTA GTGGACTCAC TTATTTAAAA TCCCTTTACC TGGATGGAAA CCAGCTACTA 
GAGATACCGC AGGGCCTCCC GCCTAGCTTA CAGCTTCTCA GCCTTGAGGC CAACAACATC 
TTTTCCATCA GAAAAGAGAA TCTAACAGAA CTGGCCAACA TAGAAATACT CTACCTGGGC 
CAAAACTGTT ATTATCGAAA TCCTTGTTAT GTTTCATATT CAATAGAGAA AGATGCCTTC 
CTAAACTTGA CAAAGTTAAA AGTGCTCTCC CTGAAAGATA ACAATGTCAC AGCCGTCCCT 
ACTGTTTTGC CATCTACTTT AACAGAACTA TATCTCTACA ACAACATGAT TGCAAAAATC 
CAAGAAGATG ATTTTAATAA CCTCAACCAA TTACAAATTC TTGACCTAAG TGGAAATTGC 
CCTCGTTGTT ATAATGCCCC ATTTCCTTGT GCGCCGTGTA AAAATAATTC TCCCCTACAG 
ATCCCTGTAA ATGCTTTTGA TGCGCTGACA GAATTAAAAG TTTTACGTCT ACACAGTAAC 
TCTCTTCAGC ATGTGCCCCC AAGATGGTTT AAGAACATCA ACAAACTCCA GGAACTGGAT 
CTGTCCCAAA ACTTCTTGGC CAAAGAAATT GGGGATGCTA AATTTCTGCA TTTTCTCCCC 
AGCCTCATCC AATTGGATCT GTCTTTCAAT TTTGAACTTC AGGTCTATCG TGCATCTATG 
AATCTATCAC AAGCATTTTC TTCACTGAAA AGCCTGAAAA TTCTGCGGAT CAGAGGATAT 
GTCTTTAAAG AGTTGAAAAG CTTTAACCTC TCGCCATTAC ATAATCTTCA AAATCTTGAA 
GTTCTTGATC TTGGCACTAA CTTTATAAAA ATTGCTAACC TCAGCATGTT TAAACAATTT 
AAAAGACTGA AAGTCATAGA TCTTTCAGTG AATAAAATAT CACCTTCAGG AGATTCAAGT 
GAAGTTGGCT TCTGCTCAAA TGCCAGAACT TCTGTAGAAA GTTATGAACC CCAGGTCCTG 
GAACAATTAC ATTATTTCAG ATATGATAAG TATGCAAGGA GTTGCAGATT CAAAAACAAA 
GAGGCTTCTT TCATGTCTGT TAATGAAAGC TGCTACAAGT ATGGGCAGAC CTTGGATCTA 
AGTAAAAATA GTATATTTTT TGTCAAGTCC TCTGATTTTC AGCATCTTTC TTTCCTCAAA 
TGCCTGAATC TGTCAGGAAA TCTCATTAGC CAAACTCTTA ATGGCAGTGA ATTCCAACCT 
TTAGCAGAGC TGAGATATTT GGACTTCTCC AACAACCGGC TTGATTTACT CCATTCAACA 
GCATTTGAAG AGCTTCACAA ACTGGAAGTT CTGGATATAA GCAGTAATAG CCATTATTTT 
CAATCAGAAG GAATTACTCA TATGCTAAAC TTTACCAAGA ACCTAAAGGT TCTGCAGAAA 
CTGATGATGA ACGACAATGA CATCTCTTCC TCCACCAGCA GGACCATGGA GAGTGAGTCT 
CTTAGAACTC TGGAATTCAG AGGAAATCAC TTAGATGTTT TATGGAGAGA AGGTGATAAC 
AGATACTTAC AATTATTCAA GAATCTGCTA AAATTAGAGG AATTAGACAT CTCTAAAAAT 
TCCCTAAGTT TCTTGCCTTC TGGAGTTTTT GATGGTATGC CTCCAAATCT AAAGAATCTC 
TCTTTGGCCA AAAATGGGCT CAAATCTTTC AGTTGGAAGA AACTCCAGTG TCTAAAGAAC 
CTGGAAACTT TGGACCTCAG CCACAACCAA CTGACCACTG TCCCTGAGAG ATTATCCAAC 
TGTTCCAGAA GCCTCAAGAA TCTGATTCTT AAGAATAATC AAATCAGGAG TCTGACGAAG 
TATTTTCTAC AAGATGCCTT CCAGTTGCGA TATCTGGATC TCAGCTCAAA TAAAATCCAG 
ATGATCCAAA AGACCAGCTT CCCAGAAAAT GTCCTCAACA ATCTGAAGAT GTTGCTTTTG 
CATCATAATC GGTTTCTGTG CACCTGTGAT GCTGTGTGGT TTGTCTGGTG GGTTAACCAT 
ACGGAGGTGA CTATTCCTTA CCTGGCCACA GATGTGACTT GTGTGGGGCC AGGAGCACAC 
AAGGGCCAAA GTGTGATCTC CCTGGATCTG TACACCTGTG AGTTAGATCT GACTAACCTG 
ATTCTGTTCT CACTTTCCAT ATCTGTATCT CTCTTTCTCA TGGTGATGAT GACAGCAAGT 
CACCTCTATT TCTGGGATGT GTGGTATATT TACCATTTCT GTAAGGCCAA GATAAAGGGG 
TATCAGCGTC TAATATCACC AGACTGTTGC TATGATGCTT TTATTGTGTA TGACACTAAA 
GACCCAGCTG TGACCGAGTG GGTTTTGGCT GAGCTGGTGG CCAAACTGGA AGACCCAAGA 
GAGAAACATT TTAATTTATG TCTCGAGGAA AGGGACTGGT TACCAGGGCA GCCAGTTCTG 
GAAAACCTTT CCCAGAGCAT ACAGCTTAGC AAAAAGACAG TGTTTGTGAT GACAGACAAG 
TATGCAAAGA CTGAAAATTT TAAGATAGCA TTTTACTTGT CCCATCAGAG GCTCATGGAT 
GAAAAAGTTG ATGTGATTAT CTTGATATTT CTTGAGAAGC CCTTTCAGAA GTCCAAGTTC 
CTCCAGCTCC GGAAAAGGCT CTGTGGGAGT TCTGTCCTTG AGTGGCCAAC AAACCCGCAA 
GCTCACCCAT ACTTCTGGCA GTGTCTAAAG AACGCCCTGG CCACAGACAA TCATGTGGCC 
TATAGTCAGG TGTTCAAGGA AACGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057646.1
CDS	151..3300
Translation	MVFPMWTLKRQILILFNIILISKLLGARWFPKTLPCDVTLDVPKNHVIVDCTDKHLTEIPGGIPTNTTNLTLTINHIPDISPASFHRLDHLVEIDFRCNCVPIPLGSKNNMCIKRLQIKPRSFSGLTYLKSLYLDGNQLLEIPQGLPPSLQLLSLEANNIFSIRKENLTELANIEILYLGQNCYYRNPCYVSYSIEKDAFLNLTKLKVLSLKDNNVTAVPTVLPSTLTELYLYNNMIAKIQEDDFNNLNQLQILDLSGNCPRCYNAPFPCAPCKNNSPLQIPVNAFDALTELKVLRLHSNSLQHVPPRWFKNINKLQELDLSQNFLAKEIGDAKFLHFLPSLIQLDLSFNFELQVYRASMNLSQAFSSLKSLKILRIRGYVFKELKSFNLSPLHNLQNLEVLDLGTNFIKIANLSMFKQFKRLKVIDLSVNKISPSGDSSEVGFCSNARTSVESYEPQVLEQLHYFRYDKYARSCRFKNKEASFMSVNESCYKYGQTLDLSKNSIFFVKSSDFQHLSFLKCLNLSGNLISQTLNGSEFQPLAELRYLDFSNNRLDLLHSTAFEELHKLEVLDISSNSHYFQSEGITHMLNFTKNLKVLQKLMMNDNDISSSTSRTMESESLRTLEFRGNHLDVLWREGDNRYLQLFKNLLKLEELDISKNSLSFLPSGVFDGMPPNLKNLSLAKNGLKSFSWKKLQCLKNLETLDLSHNQLTTVPERLSNCSRSLKNLILKNNQIRSLTKYFLQDAFQLRYLDLSSNKIQMIQKTSFPENVLNNLKMLLLHHNRFLCTCDAVWFVWWVNHTEVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTNLILFSLSISVSLFLMVMMTASHLYFWDVWYIYHFCKAKIKGYQRLISPDCCYDAFIVYDTKDPAVTEWVLAELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFYLSHQRLMDEKVDVIILIFLEKPFQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLKNALATDNHVAYSQVFKETV

Target ORF information:

RefSeq Version	NM_016562.4
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 7 (TLR7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016562.4

ORF Insert Sequence:

ATGGTGTTTC CAATGTGGAC ACTGAAGAGA CAAATTCTTA TCCTTTTTAA CATAATCCTA 
ATTTCCAAAC TCCTTGGGGC TAGATGGTTT CCTAAAACTC TGCCCTGTGA TGTCACTCTG 
GATGTTCCAA AGAACCATGT GATCGTGGAC TGCACAGACA AGCATTTGAC AGAAATTCCT 
GGAGGTATTC CCACGAACAC CACGAACCTC ACCCTCACCA TTAACCACAT ACCAGACATC 
TCCCCAGCGT CCTTTCACAG ACTGGACCAT CTGGTAGAGA TCGATTTCAG ATGCAACTGT 
GTACCTATTC CACTGGGGTC AAAAAACAAC ATGTGCATCA AGAGGCTGCA GATTAAACCC 
AGAAGCTTTA GTGGACTCAC TTATTTAAAA TCCCTTTACC TGGATGGAAA CCAGCTACTA 
GAGATACCGC AGGGCCTCCC GCCTAGCTTA CAGCTTCTCA GCCTTGAGGC CAACAACATC 
TTTTCCATCA GAAAAGAGAA TCTAACAGAA CTGGCCAACA TAGAAATACT CTACCTGGGC 
CAAAACTGTT ATTATCGAAA TCCTTGTTAT GTTTCATATT CAATAGAGAA AGATGCCTTC 
CTAAACTTGA CAAAGTTAAA AGTGCTCTCC CTGAAAGATA ACAATGTCAC AGCCGTCCCT 
ACTGTTTTGC CATCTACTTT AACAGAACTA TATCTCTACA ACAACATGAT TGCAAAAATC 
CAAGAAGATG ATTTTAATAA CCTCAACCAA TTACAAATTC TTGACCTAAG TGGAAATTGC 
CCTCGTTGTT ATAATGCCCC ATTTCCTTGT GCGCCGTGTA AAAATAATTC TCCCCTACAG 
ATCCCTGTAA ATGCTTTTGA TGCGCTGACA GAATTAAAAG TTTTACGTCT ACACAGTAAC 
TCTCTTCAGC ATGTGCCCCC AAGATGGTTT AAGAACATCA ACAAACTCCA GGAACTGGAT 
CTGTCCCAAA ACTTCTTGGC CAAAGAAATT GGGGATGCTA AATTTCTGCA TTTTCTCCCC 
AGCCTCATCC AATTGGATCT GTCTTTCAAT TTTGAACTTC AGGTCTATCG TGCATCTATG 
AATCTATCAC AAGCATTTTC TTCACTGAAA AGCCTGAAAA TTCTGCGGAT CAGAGGATAT 
GTCTTTAAAG AGTTGAAAAG CTTTAACCTC TCGCCATTAC ATAATCTTCA AAATCTTGAA 
GTTCTTGATC TTGGCACTAA CTTTATAAAA ATTGCTAACC TCAGCATGTT TAAACAATTT 
AAAAGACTGA AAGTCATAGA TCTTTCAGTG AATAAAATAT CACCTTCAGG AGATTCAAGT 
GAAGTTGGCT TCTGCTCAAA TGCCAGAACT TCTGTAGAAA GTTATGAACC CCAGGTCCTG 
GAACAATTAC ATTATTTCAG ATATGATAAG TATGCAAGGA GTTGCAGATT CAAAAACAAA 
GAGGCTTCTT TCATGTCTGT TAATGAAAGC TGCTACAAGT ATGGGCAGAC CTTGGATCTA 
AGTAAAAATA GTATATTTTT TGTCAAGTCC TCTGATTTTC AGCATCTTTC TTTCCTCAAA 
TGCCTGAATC TGTCAGGAAA TCTCATTAGC CAAACTCTTA ATGGCAGTGA ATTCCAACCT 
TTAGCAGAGC TGAGATATTT GGACTTCTCC AACAACCGGC TTGATTTACT CCATTCAACA 
GCATTTGAAG AGCTTCACAA ACTGGAAGTT CTGGATATAA GCAGTAATAG CCATTATTTT 
CAATCAGAAG GAATTACTCA TATGCTAAAC TTTACCAAGA ACCTAAAGGT TCTGCAGAAA 
CTGATGATGA ACGACAATGA CATCTCTTCC TCCACCAGCA GGACCATGGA GAGTGAGTCT 
CTTAGAACTC TGGAATTCAG AGGAAATCAC TTAGATGTTT TATGGAGAGA AGGTGATAAC 
AGATACTTAC AATTATTCAA GAATCTGCTA AAATTAGAGG AATTAGACAT CTCTAAAAAT 
TCCCTAAGTT TCTTGCCTTC TGGAGTTTTT GATGGTATGC CTCCAAATCT AAAGAATCTC 
TCTTTGGCCA AAAATGGGCT CAAATCTTTC AGTTGGAAGA AACTCCAGTG TCTAAAGAAC 
CTGGAAACTT TGGACCTCAG CCACAACCAA CTGACCACTG TCCCTGAGAG ATTATCCAAC 
TGTTCCAGAA GCCTCAAGAA TCTGATTCTT AAGAATAATC AAATCAGGAG TCTGACGAAG 
TATTTTCTAC AAGATGCCTT CCAGTTGCGA TATCTGGATC TCAGCTCAAA TAAAATCCAG 
ATGATCCAAA AGACCAGCTT CCCAGAAAAT GTCCTCAACA ATCTGAAGAT GTTGCTTTTG 
CATCATAATC GGTTTCTGTG CACCTGTGAT GCTGTGTGGT TTGTCTGGTG GGTTAACCAT 
ACGGAGGTGA CTATTCCTTA CCTGGCCACA GATGTGACTT GTGTGGGGCC AGGAGCACAC 
AAGGGCCAAA GTGTGATCTC CCTGGATCTG TACACCTGTG AGTTAGATCT GACTAACCTG 
ATTCTGTTCT CACTTTCCAT ATCTGTATCT CTCTTTCTCA TGGTGATGAT GACAGCAAGT 
CACCTCTATT TCTGGGATGT GTGGTATATT TACCATTTCT GTAAGGCCAA GATAAAGGGG 
TATCAGCGTC TAATATCACC AGACTGTTGC TATGATGCTT TTATTGTGTA TGACACTAAA 
GACCCAGCTG TGACCGAGTG GGTTTTGGCT GAGCTGGTGG CCAAACTGGA AGACCCAAGA 
GAGAAACATT TTAATTTATG TCTCGAGGAA AGGGACTGGT TACCAGGGCA GCCAGTTCTG 
GAAAACCTTT CCCAGAGCAT ACAGCTTAGC AAAAAGACAG TGTTTGTGAT GACAGACAAG 
TATGCAAAGA CTGAAAATTT TAAGATAGCA TTTTACTTGT CCCATCAGAG GCTCATGGAT 
GAAAAAGTTG ATGTGATTAT CTTGATATTT CTTGAGAAGC CCTTTCAGAA GTCCAAGTTC 
CTCCAGCTCC GGAAAAGGCT CTGTGGGAGT TCTGTCCTTG AGTGGCCAAC AAACCCGCAA 
GCTCACCCAT ACTTCTGGCA GTGTCTAAAG AACGCCCTGG CCACAGACAA TCATGTGGCC 
TATAGTCAGG TGTTCAAGGA AACGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR7 ( NM_016562.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057646.1 Homo sapiens toll like receptor 7 (TLR7), mRNA.

Price & Availability↑

CloneID	OHu19456
Clone ID Related Accession (Same CDS sequence)	NM_016562.4 , NM_016562.3
Accession Version	NM_016562.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	toll-like receptor 7 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033651.1 and AF240467.1. This sequence is a reference standard in the RefSeqGene project. On Jun 17, 2005 this sequence version replaced NM_016562.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF240467.1, SRR1163655.595483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTTTC CAATGTGGAC ACTGAAGAGA CAAATTCTTA TCCTTTTTAA CATAATCCTA 
ATTTCCAAAC TCCTTGGGGC TAGATGGTTT CCTAAAACTC TGCCCTGTGA TGTCACTCTG 
GATGTTCCAA AGAACCATGT GATCGTGGAC TGCACAGACA AGCATTTGAC AGAAATTCCT 
GGAGGTATTC CCACGAACAC CACGAACCTC ACCCTCACCA TTAACCACAT ACCAGACATC 
TCCCCAGCGT CCTTTCACAG ACTGGACCAT CTGGTAGAGA TCGATTTCAG ATGCAACTGT 
GTACCTATTC CACTGGGGTC AAAAAACAAC ATGTGCATCA AGAGGCTGCA GATTAAACCC 
AGAAGCTTTA GTGGACTCAC TTATTTAAAA TCCCTTTACC TGGATGGAAA CCAGCTACTA 
GAGATACCGC AGGGCCTCCC GCCTAGCTTA CAGCTTCTCA GCCTTGAGGC CAACAACATC 
TTTTCCATCA GAAAAGAGAA TCTAACAGAA CTGGCCAACA TAGAAATACT CTACCTGGGC 
CAAAACTGTT ATTATCGAAA TCCTTGTTAT GTTTCATATT CAATAGAGAA AGATGCCTTC 
CTAAACTTGA CAAAGTTAAA AGTGCTCTCC CTGAAAGATA ACAATGTCAC AGCCGTCCCT 
ACTGTTTTGC CATCTACTTT AACAGAACTA TATCTCTACA ACAACATGAT TGCAAAAATC 
CAAGAAGATG ATTTTAATAA CCTCAACCAA TTACAAATTC TTGACCTAAG TGGAAATTGC 
CCTCGTTGTT ATAATGCCCC ATTTCCTTGT GCGCCGTGTA AAAATAATTC TCCCCTACAG 
ATCCCTGTAA ATGCTTTTGA TGCGCTGACA GAATTAAAAG TTTTACGTCT ACACAGTAAC 
TCTCTTCAGC ATGTGCCCCC AAGATGGTTT AAGAACATCA ACAAACTCCA GGAACTGGAT 
CTGTCCCAAA ACTTCTTGGC CAAAGAAATT GGGGATGCTA AATTTCTGCA TTTTCTCCCC 
AGCCTCATCC AATTGGATCT GTCTTTCAAT TTTGAACTTC AGGTCTATCG TGCATCTATG 
AATCTATCAC AAGCATTTTC TTCACTGAAA AGCCTGAAAA TTCTGCGGAT CAGAGGATAT 
GTCTTTAAAG AGTTGAAAAG CTTTAACCTC TCGCCATTAC ATAATCTTCA AAATCTTGAA 
GTTCTTGATC TTGGCACTAA CTTTATAAAA ATTGCTAACC TCAGCATGTT TAAACAATTT 
AAAAGACTGA AAGTCATAGA TCTTTCAGTG AATAAAATAT CACCTTCAGG AGATTCAAGT 
GAAGTTGGCT TCTGCTCAAA TGCCAGAACT TCTGTAGAAA GTTATGAACC CCAGGTCCTG 
GAACAATTAC ATTATTTCAG ATATGATAAG TATGCAAGGA GTTGCAGATT CAAAAACAAA 
GAGGCTTCTT TCATGTCTGT TAATGAAAGC TGCTACAAGT ATGGGCAGAC CTTGGATCTA 
AGTAAAAATA GTATATTTTT TGTCAAGTCC TCTGATTTTC AGCATCTTTC TTTCCTCAAA 
TGCCTGAATC TGTCAGGAAA TCTCATTAGC CAAACTCTTA ATGGCAGTGA ATTCCAACCT 
TTAGCAGAGC TGAGATATTT GGACTTCTCC AACAACCGGC TTGATTTACT CCATTCAACA 
GCATTTGAAG AGCTTCACAA ACTGGAAGTT CTGGATATAA GCAGTAATAG CCATTATTTT 
CAATCAGAAG GAATTACTCA TATGCTAAAC TTTACCAAGA ACCTAAAGGT TCTGCAGAAA 
CTGATGATGA ACGACAATGA CATCTCTTCC TCCACCAGCA GGACCATGGA GAGTGAGTCT 
CTTAGAACTC TGGAATTCAG AGGAAATCAC TTAGATGTTT TATGGAGAGA AGGTGATAAC 
AGATACTTAC AATTATTCAA GAATCTGCTA AAATTAGAGG AATTAGACAT CTCTAAAAAT 
TCCCTAAGTT TCTTGCCTTC TGGAGTTTTT GATGGTATGC CTCCAAATCT AAAGAATCTC 
TCTTTGGCCA AAAATGGGCT CAAATCTTTC AGTTGGAAGA AACTCCAGTG TCTAAAGAAC 
CTGGAAACTT TGGACCTCAG CCACAACCAA CTGACCACTG TCCCTGAGAG ATTATCCAAC 
TGTTCCAGAA GCCTCAAGAA TCTGATTCTT AAGAATAATC AAATCAGGAG TCTGACGAAG 
TATTTTCTAC AAGATGCCTT CCAGTTGCGA TATCTGGATC TCAGCTCAAA TAAAATCCAG 
ATGATCCAAA AGACCAGCTT CCCAGAAAAT GTCCTCAACA ATCTGAAGAT GTTGCTTTTG 
CATCATAATC GGTTTCTGTG CACCTGTGAT GCTGTGTGGT TTGTCTGGTG GGTTAACCAT 
ACGGAGGTGA CTATTCCTTA CCTGGCCACA GATGTGACTT GTGTGGGGCC AGGAGCACAC 
AAGGGCCAAA GTGTGATCTC CCTGGATCTG TACACCTGTG AGTTAGATCT GACTAACCTG 
ATTCTGTTCT CACTTTCCAT ATCTGTATCT CTCTTTCTCA TGGTGATGAT GACAGCAAGT 
CACCTCTATT TCTGGGATGT GTGGTATATT TACCATTTCT GTAAGGCCAA GATAAAGGGG 
TATCAGCGTC TAATATCACC AGACTGTTGC TATGATGCTT TTATTGTGTA TGACACTAAA 
GACCCAGCTG TGACCGAGTG GGTTTTGGCT GAGCTGGTGG CCAAACTGGA AGACCCAAGA 
GAGAAACATT TTAATTTATG TCTCGAGGAA AGGGACTGGT TACCAGGGCA GCCAGTTCTG 
GAAAACCTTT CCCAGAGCAT ACAGCTTAGC AAAAAGACAG TGTTTGTGAT GACAGACAAG 
TATGCAAAGA CTGAAAATTT TAAGATAGCA TTTTACTTGT CCCATCAGAG GCTCATGGAT 
GAAAAAGTTG ATGTGATTAT CTTGATATTT CTTGAGAAGC CCTTTCAGAA GTCCAAGTTC 
CTCCAGCTCC GGAAAAGGCT CTGTGGGAGT TCTGTCCTTG AGTGGCCAAC AAACCCGCAA 
GCTCACCCAT ACTTCTGGCA GTGTCTAAAG AACGCCCTGG CCACAGACAA TCATGTGGCC 
TATAGTCAGG TGTTCAAGGA AACGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057646.1
CDS	140..3289
Translation	MVFPMWTLKRQILILFNIILISKLLGARWFPKTLPCDVTLDVPKNHVIVDCTDKHLTEIPGGIPTNTTNLTLTINHIPDISPASFHRLDHLVEIDFRCNCVPIPLGSKNNMCIKRLQIKPRSFSGLTYLKSLYLDGNQLLEIPQGLPPSLQLLSLEANNIFSIRKENLTELANIEILYLGQNCYYRNPCYVSYSIEKDAFLNLTKLKVLSLKDNNVTAVPTVLPSTLTELYLYNNMIAKIQEDDFNNLNQLQILDLSGNCPRCYNAPFPCAPCKNNSPLQIPVNAFDALTELKVLRLHSNSLQHVPPRWFKNINKLQELDLSQNFLAKEIGDAKFLHFLPSLIQLDLSFNFELQVYRASMNLSQAFSSLKSLKILRIRGYVFKELKSFNLSPLHNLQNLEVLDLGTNFIKIANLSMFKQFKRLKVIDLSVNKISPSGDSSEVGFCSNARTSVESYEPQVLEQLHYFRYDKYARSCRFKNKEASFMSVNESCYKYGQTLDLSKNSIFFVKSSDFQHLSFLKCLNLSGNLISQTLNGSEFQPLAELRYLDFSNNRLDLLHSTAFEELHKLEVLDISSNSHYFQSEGITHMLNFTKNLKVLQKLMMNDNDISSSTSRTMESESLRTLEFRGNHLDVLWREGDNRYLQLFKNLLKLEELDISKNSLSFLPSGVFDGMPPNLKNLSLAKNGLKSFSWKKLQCLKNLETLDLSHNQLTTVPERLSNCSRSLKNLILKNNQIRSLTKYFLQDAFQLRYLDLSSNKIQMIQKTSFPENVLNNLKMLLLHHNRFLCTCDAVWFVWWVNHTEVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTNLILFSLSISVSLFLMVMMTASHLYFWDVWYIYHFCKAKIKGYQRLISPDCCYDAFIVYDTKDPAVTEWVLAELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFYLSHQRLMDEKVDVIILIFLEKPFQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLKNALATDNHVAYSQVFKETV

Target ORF information:

RefSeq Version	NM_016562.3
Organism	Homo sapiens(human)
Definition	Homo sapiens toll like receptor 7 (TLR7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016562.3

ORF Insert Sequence:

ATGGTGTTTC CAATGTGGAC ACTGAAGAGA CAAATTCTTA TCCTTTTTAA CATAATCCTA 
ATTTCCAAAC TCCTTGGGGC TAGATGGTTT CCTAAAACTC TGCCCTGTGA TGTCACTCTG 
GATGTTCCAA AGAACCATGT GATCGTGGAC TGCACAGACA AGCATTTGAC AGAAATTCCT 
GGAGGTATTC CCACGAACAC CACGAACCTC ACCCTCACCA TTAACCACAT ACCAGACATC 
TCCCCAGCGT CCTTTCACAG ACTGGACCAT CTGGTAGAGA TCGATTTCAG ATGCAACTGT 
GTACCTATTC CACTGGGGTC AAAAAACAAC ATGTGCATCA AGAGGCTGCA GATTAAACCC 
AGAAGCTTTA GTGGACTCAC TTATTTAAAA TCCCTTTACC TGGATGGAAA CCAGCTACTA 
GAGATACCGC AGGGCCTCCC GCCTAGCTTA CAGCTTCTCA GCCTTGAGGC CAACAACATC 
TTTTCCATCA GAAAAGAGAA TCTAACAGAA CTGGCCAACA TAGAAATACT CTACCTGGGC 
CAAAACTGTT ATTATCGAAA TCCTTGTTAT GTTTCATATT CAATAGAGAA AGATGCCTTC 
CTAAACTTGA CAAAGTTAAA AGTGCTCTCC CTGAAAGATA ACAATGTCAC AGCCGTCCCT 
ACTGTTTTGC CATCTACTTT AACAGAACTA TATCTCTACA ACAACATGAT TGCAAAAATC 
CAAGAAGATG ATTTTAATAA CCTCAACCAA TTACAAATTC TTGACCTAAG TGGAAATTGC 
CCTCGTTGTT ATAATGCCCC ATTTCCTTGT GCGCCGTGTA AAAATAATTC TCCCCTACAG 
ATCCCTGTAA ATGCTTTTGA TGCGCTGACA GAATTAAAAG TTTTACGTCT ACACAGTAAC 
TCTCTTCAGC ATGTGCCCCC AAGATGGTTT AAGAACATCA ACAAACTCCA GGAACTGGAT 
CTGTCCCAAA ACTTCTTGGC CAAAGAAATT GGGGATGCTA AATTTCTGCA TTTTCTCCCC 
AGCCTCATCC AATTGGATCT GTCTTTCAAT TTTGAACTTC AGGTCTATCG TGCATCTATG 
AATCTATCAC AAGCATTTTC TTCACTGAAA AGCCTGAAAA TTCTGCGGAT CAGAGGATAT 
GTCTTTAAAG AGTTGAAAAG CTTTAACCTC TCGCCATTAC ATAATCTTCA AAATCTTGAA 
GTTCTTGATC TTGGCACTAA CTTTATAAAA ATTGCTAACC TCAGCATGTT TAAACAATTT 
AAAAGACTGA AAGTCATAGA TCTTTCAGTG AATAAAATAT CACCTTCAGG AGATTCAAGT 
GAAGTTGGCT TCTGCTCAAA TGCCAGAACT TCTGTAGAAA GTTATGAACC CCAGGTCCTG 
GAACAATTAC ATTATTTCAG ATATGATAAG TATGCAAGGA GTTGCAGATT CAAAAACAAA 
GAGGCTTCTT TCATGTCTGT TAATGAAAGC TGCTACAAGT ATGGGCAGAC CTTGGATCTA 
AGTAAAAATA GTATATTTTT TGTCAAGTCC TCTGATTTTC AGCATCTTTC TTTCCTCAAA 
TGCCTGAATC TGTCAGGAAA TCTCATTAGC CAAACTCTTA ATGGCAGTGA ATTCCAACCT 
TTAGCAGAGC TGAGATATTT GGACTTCTCC AACAACCGGC TTGATTTACT CCATTCAACA 
GCATTTGAAG AGCTTCACAA ACTGGAAGTT CTGGATATAA GCAGTAATAG CCATTATTTT 
CAATCAGAAG GAATTACTCA TATGCTAAAC TTTACCAAGA ACCTAAAGGT TCTGCAGAAA 
CTGATGATGA ACGACAATGA CATCTCTTCC TCCACCAGCA GGACCATGGA GAGTGAGTCT 
CTTAGAACTC TGGAATTCAG AGGAAATCAC TTAGATGTTT TATGGAGAGA AGGTGATAAC 
AGATACTTAC AATTATTCAA GAATCTGCTA AAATTAGAGG AATTAGACAT CTCTAAAAAT 
TCCCTAAGTT TCTTGCCTTC TGGAGTTTTT GATGGTATGC CTCCAAATCT AAAGAATCTC 
TCTTTGGCCA AAAATGGGCT CAAATCTTTC AGTTGGAAGA AACTCCAGTG TCTAAAGAAC 
CTGGAAACTT TGGACCTCAG CCACAACCAA CTGACCACTG TCCCTGAGAG ATTATCCAAC 
TGTTCCAGAA GCCTCAAGAA TCTGATTCTT AAGAATAATC AAATCAGGAG TCTGACGAAG 
TATTTTCTAC AAGATGCCTT CCAGTTGCGA TATCTGGATC TCAGCTCAAA TAAAATCCAG 
ATGATCCAAA AGACCAGCTT CCCAGAAAAT GTCCTCAACA ATCTGAAGAT GTTGCTTTTG 
CATCATAATC GGTTTCTGTG CACCTGTGAT GCTGTGTGGT TTGTCTGGTG GGTTAACCAT 
ACGGAGGTGA CTATTCCTTA CCTGGCCACA GATGTGACTT GTGTGGGGCC AGGAGCACAC 
AAGGGCCAAA GTGTGATCTC CCTGGATCTG TACACCTGTG AGTTAGATCT GACTAACCTG 
ATTCTGTTCT CACTTTCCAT ATCTGTATCT CTCTTTCTCA TGGTGATGAT GACAGCAAGT 
CACCTCTATT TCTGGGATGT GTGGTATATT TACCATTTCT GTAAGGCCAA GATAAAGGGG 
TATCAGCGTC TAATATCACC AGACTGTTGC TATGATGCTT TTATTGTGTA TGACACTAAA 
GACCCAGCTG TGACCGAGTG GGTTTTGGCT GAGCTGGTGG CCAAACTGGA AGACCCAAGA 
GAGAAACATT TTAATTTATG TCTCGAGGAA AGGGACTGGT TACCAGGGCA GCCAGTTCTG 
GAAAACCTTT CCCAGAGCAT ACAGCTTAGC AAAAAGACAG TGTTTGTGAT GACAGACAAG 
TATGCAAAGA CTGAAAATTT TAAGATAGCA TTTTACTTGT CCCATCAGAG GCTCATGGAT 
GAAAAAGTTG ATGTGATTAT CTTGATATTT CTTGAGAAGC CCTTTCAGAA GTCCAAGTTC 
CTCCAGCTCC GGAAAAGGCT CTGTGGGAGT TCTGTCCTTG AGTGGCCAAC AAACCCGCAA 
GCTCACCCAT ACTTCTGGCA GTGTCTAAAG AACGCCCTGG CCACAGACAA TCATGTGGCC 
TATAGTCAGG TGTTCAAGGA AACGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TLR7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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