MRPL30 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL30
Entrez Gene ID	51263
Full Name	mitochondrial ribosomal protein L30
Synonyms	L28MT,L30MT,MRP-L28,MRP-L30,MRPL28,MRPL28M,RPML28
General protein information	Preferred Names mitochondrial ribosomal protein L30 Names 39S ribosomal protein L30, mitochondrial 39S ribosomal protein L28, mitochondrial mitochondrial large ribosomal subunit protein uL30m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. [provided by RefSeq, Mar 2011].
Disorder MIM:	611838

mRNA and Protein(s)

mRNA	Protein	Name
NM_145212.4	NP_660213.1	39S ribosomal protein L30, mitochondrial precursor
NM_145212.3	NP_660213.1	39S ribosomal protein L30, mitochondrial precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100910006	XP_003750730.1
Mus musculus (house mouse)	Mrpl30	NP_081374.1
Danio rerio (zebrafish)	mrpl30	NP_001018597.1
Xenopus tropicalis (tropical clawed frog)	mrpl30	NP_001016148.1
Homo sapiens (human)	MRPL30	NP_660213.1
Pan troglodytes (chimpanzee)	MRPL30	XP_515648.3
	LOC713711	XP_001103161.2
Canis lupus familiaris (dog)	MRPL30	XP_005626086.1
Bos taurus (cattle)	MRPL30	NP_001030520.1
Rattus norvegicus (Norway rat)	Mrpl30	NP_001100373.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.

Nature genetics43(10)977-83(2011 Sep)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11047	NM_145212.4 Latest version!	Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu11047	NM_145212.3	Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL30 ( NM_145212.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_660213.1 Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11047
Clone ID Related Accession (Same CDS sequence)	NM_145212.4 , NM_145212.3
Accession Version	NM_145212.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	39S ribosomal protein L30, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022391.1, BU533068.1, AC092587.3, AC079447.4, BC013822.1 and DB353963.1. On Nov 22, 2018 this sequence version replaced NM_145212.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.188382.1, SRR1660803.206991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000338148.8/ ENSP00000338057.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGGA TTTTGCGCTT AGTAGTTCAA TGGCCCCCAG GCAGACTACA GACTGTGACA 
AAAGGTGTGG AGTCTCTTAT TTGTACAGAT TGGATTCGTC ACAAATTCAC CAGATCAAGA 
ATTCCAGAAA AAGTGTTTCA GGCCTCACCT GAAGATCATG AAAAATACGG TGGGGATCCA 
CAGAACCCTC ATAAACTGCA TATTGTTACC AGAATAAAAA GTACAAGAAG ACGTCCATAT 
TGGGAAAAAG ATATAATAAA GATGCTTGGA TTAGAAAAAG CACATACCCC TCAAGTTCAC 
AAGAATATCC CTTCAGTGAA TGCAAAATTG AAAGTAGTTA AGCATTTGAT AAGAATCAAG 
CCCTTGAAGT TGCCACAAGG ACTTCCAGCA GAGGAGAACA TGTCTAACAC GTGCCTCAAA 
AGCACTGGGG AGTTAGTAGT GCAGTGGCAT CTGAAACCTG TGGAGCAGAA AGCACATGAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660213.1
CDS	94..579
Translation	MAGILRLVVQWPPGRLQTVTKGVESLICTDWIRHKFTRSRIPEKVFQASPEDHEKYGGDPQNPHKLHIVTRIKSTRRRPYWEKDIIKMLGLEKAHTPQVHKNIPSVNAKLKVVKHLIRIKPLKLPQGLPAEENMSNTCLKSTGELVVQWHLKPVEQKAHES

Target ORF information:

RefSeq Version	NM_145212.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145212.4

ORF Insert Sequence:

ATGGCTGGGA TTTTGCGCTT AGTAGTTCAA TGGCCCCCAG GCAGACTACA GACTGTGACA 
AAAGGTGTGG AGTCTCTTAT TTGTACAGAT TGGATTCGTC ACAAATTCAC CAGATCAAGA 
ATTCCAGAAA AAGTGTTTCA GGCCTCACCT GAAGATCATG AAAAATACGG TGGGGATCCA 
CAGAACCCTC ATAAACTGCA TATTGTTACC AGAATAAAAA GTACAAGAAG ACGTCCATAT 
TGGGAAAAAG ATATAATAAA GATGCTTGGA TTAGAAAAAG CACATACCCC TCAAGTTCAC 
AAGAATATCC CTTCAGTGAA TGCAAAATTG AAAGTAGTTA AGCATTTGAT AAGAATCAAG 
CCCTTGAAGT TGCCACAAGG ACTTCCAGCA GAGGAGAACA TGTCTAACAC GTGCCTCAAA 
AGCACTGGGG AGTTAGTAGT GCAGTGGCAT CTGAAACCTG TGGAGCAGAA AGCACATGAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL30 ( NM_145212.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_660213.1 Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11047
Clone ID Related Accession (Same CDS sequence)	NM_145212.4 , NM_145212.3
Accession Version	NM_145212.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	39S ribosomal protein L30, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA310406.1, BC022391.1, BU533068.1, AC092587.3, AC079447.4, BC013822.1 and DB353963.1. On Mar 10, 2011 this sequence version replaced NM_145212.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DA310406.1, SRR1660807.209540.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGA TTTTGCGCTT AGTAGTTCAA TGGCCCCCAG GCAGACTACA GACTGTGACA 
AAAGGTGTGG AGTCTCTTAT TTGTACAGAT TGGATTCGTC ACAAATTCAC CAGATCAAGA 
ATTCCAGAAA AAGTGTTTCA GGCCTCACCT GAAGATCATG AAAAATACGG TGGGGATCCA 
CAGAACCCTC ATAAACTGCA TATTGTTACC AGAATAAAAA GTACAAGAAG ACGTCCATAT 
TGGGAAAAAG ATATAATAAA GATGCTTGGA TTAGAAAAAG CACATACCCC TCAAGTTCAC 
AAGAATATCC CTTCAGTGAA TGCAAAATTG AAAGTAGTTA AGCATTTGAT AAGAATCAAG 
CCCTTGAAGT TGCCACAAGG ACTTCCAGCA GAGGAGAACA TGTCTAACAC GTGCCTCAAA 
AGCACTGGGG AGTTAGTAGT GCAGTGGCAT CTGAAACCTG TGGAGCAGAA AGCACATGAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660213.1
CDS	199..684
Translation	MAGILRLVVQWPPGRLQTVTKGVESLICTDWIRHKFTRSRIPEKVFQASPEDHEKYGGDPQNPHKLHIVTRIKSTRRRPYWEKDIIKMLGLEKAHTPQVHKNIPSVNAKLKVVKHLIRIKPLKLPQGLPAEENMSNTCLKSTGELVVQWHLKPVEQKAHES

Target ORF information:

RefSeq Version	NM_145212.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L30 (MRPL30), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145212.3

ORF Insert Sequence:

ATGGCTGGGA TTTTGCGCTT AGTAGTTCAA TGGCCCCCAG GCAGACTACA GACTGTGACA 
AAAGGTGTGG AGTCTCTTAT TTGTACAGAT TGGATTCGTC ACAAATTCAC CAGATCAAGA 
ATTCCAGAAA AAGTGTTTCA GGCCTCACCT GAAGATCATG AAAAATACGG TGGGGATCCA 
CAGAACCCTC ATAAACTGCA TATTGTTACC AGAATAAAAA GTACAAGAAG ACGTCCATAT 
TGGGAAAAAG ATATAATAAA GATGCTTGGA TTAGAAAAAG CACATACCCC TCAAGTTCAC 
AAGAATATCC CTTCAGTGAA TGCAAAATTG AAAGTAGTTA AGCATTTGAT AAGAATCAAG 
CCCTTGAAGT TGCCACAAGG ACTTCCAGCA GAGGAGAACA TGTCTAACAC GTGCCTCAAA 
AGCACTGGGG AGTTAGTAGT GCAGTGGCAT CTGAAACCTG TGGAGCAGAA AGCACATGAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.
Nature genetics43(10)977-83(2011 Sep)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

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MRPL30 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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