HERC5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC5
Entrez Gene ID	51191
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 5
Synonyms	CEB1,CEBP1
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase 5 Names E3 ISG15--protein ligase HERC5 HECT domain and RCC1-like domain-containing protein 5 cyclin-E-binding protein 1 hect domain and RLD 5 probable E3 ubiquitin-protein ligase HERC5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q22.1
Summary	This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008].
Disorder MIM:	608242

mRNA and Protein(s)

mRNA	Protein	Name
NM_016323.3	NP_057407.2	E3 ISG15--protein ligase HERC5
NM_016323.4	NP_057407.2	E3 ISG15--protein ligase HERC5
XM_011532022.2	XP_011530324.2	E3 ISG15--protein ligase HERC5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HERC5	XP_003310459.1
Canis lupus familiaris (dog)	HERC5	XP_005639229.1
Homo sapiens (human)	HERC5	NP_057407.2
Bos taurus (cattle)	HERC5	NP_001095465.1

Gene Ontology
Bibliography

Related articles in PubMed

HECT Domain and RCC1-Like Domain-Containing Protein 5 (HERC-5) Gene Polymorphisms in HIV-1-Infected Individuals: A Study from India.
Shaikh N, Saxena V, Pandey S, Patil A, Thakar M
AIDS research and human retroviruses33(11)1171-1174(2017 Nov)

Covalent ISG15 conjugation positively regulates the ubiquitin E3 ligase activity of parkin.
Im E, Yoo L, Hyun M, Shin WH, Chung KC
Open biology6(8)(2016 08)

HERC5 is a prognostic biomarker for post-liver transplant recurrent human hepatocellular carcinoma.
Xue F, Higgs BW, Huang J, Morehouse C, Zhu W, Yao X, Brohawn P, Xiao Z, Sebastian Y, Liu Z, Xia Y, Shen D, Kuziora M, Dong Z, Han H, Gu Y, Gu J, Xia Q, Yao Y
Journal of translational medicine13379(2015 Dec)

Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase.
Domingues P, Bamford CG, Boutell C, McLauchlan J
The Journal of general virology96(11)3236-42(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that HERC5 mediates covalent ISG15 conjugation to parkin in mammalian cells and that ISG15 is conjugated to the Lys349 and Lys369 residues of parkin.
Title: Covalent ISG15 conjugation positively regulates the ubiquitin E3 ligase activity of parkin.

HERC5 plays a crucial role in HCC immune evasion and has clinical relevance as a reproducible prognostic marker for risk of tumor recurrence and survival in patients.
Title: HERC5 is a prognostic biomarker for post-liver transplant recurrent human hepatocellular carcinoma.

vIRF1 association with HERC5 altered ISG15 modification of cellular proteins, and knockdown of ISG15 augmented reactivation of KSHV from latency.
Title: Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 1 Interacts with a Member of the Interferon-Stimulated Gene 15 Pathway.

The inhibitory effect of ISG15 on HCV RNA replication does not require its conjugation to substrates by HERC5.
Title: Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase.

Results show that HERC5 gene may be involved in regulating the spread of non-small cell lung cancer tumors where the methylation of its promoter is correlated with number increase of disseminated tumor cells and metastases as well as survival decreased.
Title: Identification of HERC5 and its potential role in NSCLC progression.

The following HERC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu95224	XM_011532022.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu00347	NM_016323.4 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu00347	NM_016323.3	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC5 ( XM_011532022.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530324.2 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95224
Clone ID Related Accession (Same CDS sequence)	XM_011532022.2
Accession Version	XM_011532022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ISG15--protein ligase HERC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTGT CAGGGTGCCT CCTAGTGGAA CTGCAGCGTT TCCTCGAAAG CCATTCCGTC 
CAGGAGTTAA GGTGGCCGCT GCCCGCCCGG GGACACTTTC TCAGTCCAGC CCATCGGCCT 
TTACCCCAGC CAACGACCTG TTGCCTTTGG CCGCCTGCCA AGTCACTCTC ATTACAGTTT 
CCTTTTCCTT TTCGATTCCG CCCCCTAAAA ACAACAACAA ACATTATGTT TCGTTTTCCA 
CTGGAGCTGC AGGCCCCGCC CCCAAACAGC ACGTGGGGGG CGTGGCGTCC CGGCAGGGGC 
TCAGTAGCTG AGGCTGCGGT TCCCCGACGC CACGCAGCTG CGCGCAGCTG GTTCCCGCTC 
TGCAGCGCAA CGCCTGAGGC AGTGGGCGCG CTCAGTCCCG GGACCAGGCG TTCTCTCCTC 
TCGCCTCTGG GCCTGGGACC CCGCAAAGCG GCGATGGAGC GGAGGTCGCG GAGGAAGTCG 
CGGCGCAACG GGCGCTCGAC CGCGGGCAAG GCCGCCGCGA CCCAGCCCGC GAAGTCTCCG 
GGCGCACAGC TCTGGCTCTT TCCCAGCGCC GCGGGCCTCC ACCGCGCGCT GCTCCGGAGG 
GTGGAGGTGA CGCGCCAACT CTGCTGCTCG CCGGGGCGCC TCGCGGTCTT GGAACGCGGC 
GGGGCGGGCG TCCAGGTTCA CCAGCTGCTC GCCGGGAGCG GCGGCGCCCG GACGCCGAAA 
TGCATTAAAT TAGGAAAAAA CATGAAGATA CATTCCGTGG ACCAAGGAGC AGAGCACATG 
CTGATTCTCT CATCAGATGG AAAACCATTT GAGTATGACA ACTATAGCAT GAAACATCTA 
AGGTTTGAAA GCATTTTACA AGAAAAAAAA ATAATTCAGA TCACATGTGG AGATTACCAT 
TCTCTTGCAC TCTCAAAAGG TGGTGAGCTT TTTGCCTGGG GACAGAACCT GCATGGGCAG 
CTTGGAGTTG GAAGGAAATT TCCCTCAACC ACCACACCAC AGATTGTGGA GCACCTCGCA 
GGAGTACCCT TGGCTCAGAT TTCTGCCGGA GAAGCCCACA GCATGGCCTT ATCCATGTCT 
GGCAACATTT ATTCATGGGG AAAAAATGAA TGTGGACAAC TAGGCCTGGG CCACACTGAG 
AGTAAAGATG ATCCATCCCT TATTGAAGGA CTAGACAATC AGAAAGTTGA ATTTGTCGCT 
TGTGGTGGCT CTCACAGTGC CCTACTCACA CAGGATGGGC TGCTGTTTAC TTTCGGTGCT 
GGAAAACATG GGCAACTTGG TCATAATTCA ACACAGAATG AGCTAAGACC CTGTTTGGTG 
GCTGAGCTTG TTGGGTATAG AGTGACTCAG ATAGCATGTG GAAGGTGGCA CACACTTGCC 
TATGTTTCTG ATTTGGGAAA GGTCTTTTCC TTTGGTTCTG GAAAAGATGG ACAACTGGGA 
AATGGTGGAA CACGTGACCA GCTGATGCCG CTTCCAGTGA AAGTATCATC AAGTGAAGAA 
CTCAAACTTG AAAGCCATAC CTCAGAAAAG GAGTTAATAA TGATTGCTGG AGGGAATCAA 
AGCATTTTGC TCTGGATAAA GAAAGAGAAT TCATATGTTA ATCTGAAGAG GACAATTCCT 
ACTCTGAATG AAGGGACTGT AAAGAGATGG ATTGCTGATG TGGAGACTAA ACGGTGGCAG 
AGCACAAAAA GGGAAATCCA AGAGATATTT TCATCTCCTG CTTGTCTAAC TGGAAGTTTT 
TTAAGGAAAA GAAGAACTAC AGAAATGATG CCTGTTTATT TGGACTTAAA TAAAGCAAGA 
AACATCTTCA AGGAGTTAAC CCAAAAGGAC TGGATTACTA ACATGATAAC CACCTGCCTC 
AAAGATAATC TGCTCAAAAG ACTTCCATTT CATTCTCCAC CCCAAGAAGC TTTAGAAATT 
TTCTTCCTTC TCCCAGAATG TCCTATGATG CATATTTCCA ACAACTGGGA GAGCCTTGTG 
GTTCCATTTG CAAAGGTTGT TTGTAAAATG AGTGACCAGT CTTCACTGGT TCTGGAAGAG 
TATTGGGCAA CTCTGCAAGA ATCCACTTTC AGCAAACTGG TCCAGATGTT TAAAACAGCC 
GTCATATGCC AGTTGGATTA CTGGGATGAA AGTGCTGAGG AGAATGGTAA TGTTCAAGCT 
CTCCTAGAAA TGTTGAAGAA GCTGCACAGG AGTAAAAAAC ATAAAGCTTA TCTTAGGTCG 
GCAGCAATTG AGGAAGAAAG AGAGTCTGAA TTCGCTTTGA GGCCCACGTT TGATCTAACA 
GTCAGAAGGA ATCACTTGAT TGAGGATGTT TTGAATCAGC TAAGTCAATT TGAGAATGAA 
GACCTGAGGA AAGAGTTATG GGTTTCATTT AGTGGAGAAA TTGGGTATGA CCTCGGAGGA 
GTCAAGAAAG AGTTCTTCTA CTGTCTGTTT GCAGAGATGA TCCAGCCGGA ATATGGGATG 
TTCATGTATC CTGAAGGGGC TTCCTGCATG TGGTTTCCTG TCAAGCCTAA ATTTGAGAAG 
AAAAGATACT TCTTTTTTGG GGTTCTATGT GGACTTTCCC TGTTCAATTG CAATGTTGCC 
AACCTTCCTT TCCCACTGGC ACTGTTTAAG AAACTTTTGG ACCAAATGCC ATCATTGGAA 
GACTTGAAAG AACTCAGTCC TGATTTGGGA AAGAATTTGC AAACACTTCT GGATGATGAA 
GGTGATAACT TTGAGGAAGT ATTTTACATC CATTTTAATG TGCACTGGGA CAGAAACGAC 
ACAAACTTAA TTCCTAATGG AAGTAGCATA ACTGTCAACC AGACTAACAA GAGAGACTAT 
GTTTCTAAGT ATATCAATTA CATTTTCAAC GACTCTGTAA AGGCGGTTTA TGAAGAATTT 
CGGAGAGGAT TTTATAAAAT GTGCGACGAA GACATTATCA AATTATTCCA CCCCGAAGAA 
CTGAAGGATG TGATTGTTGG AAATACAGAT TATGATTGGA AAACATTTGA AAAGAATGCA 
CGTTATGAAC CAGGATATAA CAGTTCACAT CCCACCATAG TGATGTTTTG GAAGGCTTTC 
CACAAATTGA CTCTGGAAGA AAAGAAAAAA TTCCTTGTAT TTCTTACAGG AACTGACAGA 
CTACAAATGA AAGATTTAAA TAATATGAAA ATAACATTTT GCTGTCCTGA AAGTTGGAAT 
GAAAGAGACC CTATAAGAGC ACTGACATGT TTCAGTGTCC TCTTCCTCCC TAAATATTCT 
ACAATGGAAA CAGTTGAAGA AGCGCTTCAA GAAGCCATCA ACAACAACAG AGGATTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530324.2
CDS	214..3516
Translation	MRVSGCLLVELQRFLESHSVQELRWPLPARGHFLSPAHRPLPQPTTCCLWPPAKSLSLQFPFPFRFRPLKTTTNIMFRFPLELQAPPPNSTWGAWRPGRGSVAEAAVPRRHAAARSWFPLCSATPEAVGALSPGTRRSLLSPLGLGPRKAAMERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRSKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG

Target ORF information:

RefSeq Version	XM_011532022.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532022.2

ORF Insert Sequence:

ATGCGTGTGT CAGGGTGCCT CCTAGTGGAA CTGCAGCGTT TCCTCGAAAG CCATTCCGTC 
CAGGAGTTAA GGTGGCCGCT GCCCGCCCGG GGACACTTTC TCAGTCCAGC CCATCGGCCT 
TTACCCCAGC CAACGACCTG TTGCCTTTGG CCGCCTGCCA AGTCACTCTC ATTACAGTTT 
CCTTTTCCTT TTCGATTCCG CCCCCTAAAA ACAACAACAA ACATTATGTT TCGTTTTCCA 
CTGGAGCTGC AGGCCCCGCC CCCAAACAGC ACGTGGGGGG CGTGGCGTCC CGGCAGGGGC 
TCAGTAGCTG AGGCTGCGGT TCCCCGACGC CACGCAGCTG CGCGCAGCTG GTTCCCGCTC 
TGCAGCGCAA CGCCTGAGGC AGTGGGCGCG CTCAGTCCCG GGACCAGGCG TTCTCTCCTC 
TCGCCTCTGG GCCTGGGACC CCGCAAAGCG GCGATGGAGC GGAGGTCGCG GAGGAAGTCG 
CGGCGCAACG GGCGCTCGAC CGCGGGCAAG GCCGCCGCGA CCCAGCCCGC GAAGTCTCCG 
GGCGCACAGC TCTGGCTCTT TCCCAGCGCC GCGGGCCTCC ACCGCGCGCT GCTCCGGAGG 
GTGGAGGTGA CGCGCCAACT CTGCTGCTCG CCGGGGCGCC TCGCGGTCTT GGAACGCGGC 
GGGGCGGGCG TCCAGGTTCA CCAGCTGCTC GCCGGGAGCG GCGGCGCCCG GACGCCGAAA 
TGCATTAAAT TAGGAAAAAA CATGAAGATA CATTCCGTGG ACCAAGGAGC AGAGCACATG 
CTGATTCTCT CATCAGATGG AAAACCATTT GAGTATGACA ACTATAGCAT GAAACATCTA 
AGGTTTGAAA GCATTTTACA AGAAAAAAAA ATAATTCAGA TCACATGTGG AGATTACCAT 
TCTCTTGCAC TCTCAAAAGG TGGTGAGCTT TTTGCCTGGG GACAGAACCT GCATGGGCAG 
CTTGGAGTTG GAAGGAAATT TCCCTCAACC ACCACACCAC AGATTGTGGA GCACCTCGCA 
GGAGTACCCT TGGCTCAGAT TTCTGCCGGA GAAGCCCACA GCATGGCCTT ATCCATGTCT 
GGCAACATTT ATTCATGGGG AAAAAATGAA TGTGGACAAC TAGGCCTGGG CCACACTGAG 
AGTAAAGATG ATCCATCCCT TATTGAAGGA CTAGACAATC AGAAAGTTGA ATTTGTCGCT 
TGTGGTGGCT CTCACAGTGC CCTACTCACA CAGGATGGGC TGCTGTTTAC TTTCGGTGCT 
GGAAAACATG GGCAACTTGG TCATAATTCA ACACAGAATG AGCTAAGACC CTGTTTGGTG 
GCTGAGCTTG TTGGGTATAG AGTGACTCAG ATAGCATGTG GAAGGTGGCA CACACTTGCC 
TATGTTTCTG ATTTGGGAAA GGTCTTTTCC TTTGGTTCTG GAAAAGATGG ACAACTGGGA 
AATGGTGGAA CACGTGACCA GCTGATGCCG CTTCCAGTGA AAGTATCATC AAGTGAAGAA 
CTCAAACTTG AAAGCCATAC CTCAGAAAAG GAGTTAATAA TGATTGCTGG AGGGAATCAA 
AGCATTTTGC TCTGGATAAA GAAAGAGAAT TCATATGTTA ATCTGAAGAG GACAATTCCT 
ACTCTGAATG AAGGGACTGT AAAGAGATGG ATTGCTGATG TGGAGACTAA ACGGTGGCAG 
AGCACAAAAA GGGAAATCCA AGAGATATTT TCATCTCCTG CTTGTCTAAC TGGAAGTTTT 
TTAAGGAAAA GAAGAACTAC AGAAATGATG CCTGTTTATT TGGACTTAAA TAAAGCAAGA 
AACATCTTCA AGGAGTTAAC CCAAAAGGAC TGGATTACTA ACATGATAAC CACCTGCCTC 
AAAGATAATC TGCTCAAAAG ACTTCCATTT CATTCTCCAC CCCAAGAAGC TTTAGAAATT 
TTCTTCCTTC TCCCAGAATG TCCTATGATG CATATTTCCA ACAACTGGGA GAGCCTTGTG 
GTTCCATTTG CAAAGGTTGT TTGTAAAATG AGTGACCAGT CTTCACTGGT TCTGGAAGAG 
TATTGGGCAA CTCTGCAAGA ATCCACTTTC AGCAAACTGG TCCAGATGTT TAAAACAGCC 
GTCATATGCC AGTTGGATTA CTGGGATGAA AGTGCTGAGG AGAATGGTAA TGTTCAAGCT 
CTCCTAGAAA TGTTGAAGAA GCTGCACAGG AGTAAAAAAC ATAAAGCTTA TCTTAGGTCG 
GCAGCAATTG AGGAAGAAAG AGAGTCTGAA TTCGCTTTGA GGCCCACGTT TGATCTAACA 
GTCAGAAGGA ATCACTTGAT TGAGGATGTT TTGAATCAGC TAAGTCAATT TGAGAATGAA 
GACCTGAGGA AAGAGTTATG GGTTTCATTT AGTGGAGAAA TTGGGTATGA CCTCGGAGGA 
GTCAAGAAAG AGTTCTTCTA CTGTCTGTTT GCAGAGATGA TCCAGCCGGA ATATGGGATG 
TTCATGTATC CTGAAGGGGC TTCCTGCATG TGGTTTCCTG TCAAGCCTAA ATTTGAGAAG 
AAAAGATACT TCTTTTTTGG GGTTCTATGT GGACTTTCCC TGTTCAATTG CAATGTTGCC 
AACCTTCCTT TCCCACTGGC ACTGTTTAAG AAACTTTTGG ACCAAATGCC ATCATTGGAA 
GACTTGAAAG AACTCAGTCC TGATTTGGGA AAGAATTTGC AAACACTTCT GGATGATGAA 
GGTGATAACT TTGAGGAAGT ATTTTACATC CATTTTAATG TGCACTGGGA CAGAAACGAC 
ACAAACTTAA TTCCTAATGG AAGTAGCATA ACTGTCAACC AGACTAACAA GAGAGACTAT 
GTTTCTAAGT ATATCAATTA CATTTTCAAC GACTCTGTAA AGGCGGTTTA TGAAGAATTT 
CGGAGAGGAT TTTATAAAAT GTGCGACGAA GACATTATCA AATTATTCCA CCCCGAAGAA 
CTGAAGGATG TGATTGTTGG AAATACAGAT TATGATTGGA AAACATTTGA AAAGAATGCA 
CGTTATGAAC CAGGATATAA CAGTTCACAT CCCACCATAG TGATGTTTTG GAAGGCTTTC 
CACAAATTGA CTCTGGAAGA AAAGAAAAAA TTCCTTGTAT TTCTTACAGG AACTGACAGA 
CTACAAATGA AAGATTTAAA TAATATGAAA ATAACATTTT GCTGTCCTGA AAGTTGGAAT 
GAAAGAGACC CTATAAGAGC ACTGACATGT TTCAGTGTCC TCTTCCTCCC TAAATATTCT 
ACAATGGAAA CAGTTGAAGA AGCGCTTCAA GAAGCCATCA ACAACAACAG AGGATTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC5 ( NM_016323.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057407.2 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.

Price & Availability↑

CloneID	OHu00347
Clone ID Related Accession (Same CDS sequence)	NM_016323.3 , NM_016323.4
Accession Version	NM_016323.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	E3 ISG15--protein ligase HERC5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY337518.1, BC140716.1, AK302142.1, AC083829.5 and AI336946.1. On Mar 15, 2012 this sequence version replaced NM_016323.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY337518.1, AB027289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA GGTCGCGGAG GAAGTCGCGG CGCAACGGGC GCTCGACCGC GGGCAAGGCC 
GCCGCGACCC AGCCCGCGAA GTCTCCGGGC GCACAGCTCT GGCTCTTTCC CAGCGCCGCG 
GGCCTCCACC GCGCGCTGCT CCGGAGGGTG GAGGTGACGC GCCAACTCTG CTGCTCGCCG 
GGGCGCCTCG CGGTCTTGGA ACGCGGCGGG GCGGGCGTCC AGGTTCACCA GCTGCTCGCC 
GGGAGCGGCG GCGCCCGGAC GCCGAAATGC ATTAAATTAG GAAAAAACAT GAAGATACAT 
TCCGTGGACC AAGGAGCAGA GCACATGCTG ATTCTCTCAT CAGATGGAAA ACCATTTGAG 
TATGACAACT ATAGCATGAA ACATCTAAGG TTTGAAAGCA TTTTACAAGA AAAAAAAATA 
ATTCAGATCA CATGTGGAGA TTACCATTCT CTTGCACTCT CAAAAGGTGG TGAGCTTTTT 
GCCTGGGGAC AGAACCTGCA TGGGCAGCTT GGAGTTGGAA GGAAATTTCC CTCAACCACC 
ACACCACAGA TTGTGGAGCA CCTCGCAGGA GTACCCTTGG CTCAGATTTC TGCCGGAGAA 
GCCCACAGCA TGGCCTTATC CATGTCTGGC AACATTTATT CATGGGGAAA AAATGAATGT 
GGACAACTAG GCCTGGGCCA CACTGAGAGT AAAGATGATC CATCCCTTAT TGAAGGACTA 
GACAATCAGA AAGTTGAATT TGTCGCTTGT GGTGGCTCTC ACAGTGCCCT ACTCACACAG 
GATGGGCTGC TGTTTACTTT CGGTGCTGGA AAACATGGGC AACTTGGTCA TAATTCAACA 
CAGAATGAGC TAAGACCCTG TTTGGTGGCT GAGCTTGTTG GGTATAGAGT GACTCAGATA 
GCATGTGGAA GGTGGCACAC ACTTGCCTAT GTTTCTGATT TGGGAAAGGT CTTTTCCTTT 
GGTTCTGGAA AAGATGGACA ACTGGGAAAT GGTGGAACAC GTGACCAGCT GATGCCGCTT 
CCAGTGAAAG TATCATCAAG TGAAGAACTC AAACTTGAAA GCCATACCTC AGAAAAGGAG 
TTAATAATGA TTGCTGGAGG GAATCAAAGC ATTTTGCTCT GGATAAAGAA AGAGAATTCA 
TATGTTAATC TGAAGAGGAC AATTCCTACT CTGAATGAAG GGACTGTAAA GAGATGGATT 
GCTGATGTGG AGACTAAACG GTGGCAGAGC ACAAAAAGGG AAATCCAAGA GATATTTTCA 
TCTCCTGCTT GTCTAACTGG AAGTTTTTTA AGGAAAAGAA GAACTACAGA AATGATGCCT 
GTTTATTTGG ACTTAAATAA AGCAAGAAAC ATCTTCAAGG AGTTAACCCA AAAGGACTGG 
ATTACTAACA TGATAACCAC CTGCCTCAAA GATAATCTGC TCAAAAGACT TCCATTTCAT 
TCTCCACCCC AAGAAGCTTT AGAAATTTTC TTCCTTCTCC CAGAATGTCC TATGATGCAT 
ATTTCCAACA ACTGGGAGAG CCTTGTGGTT CCATTTGCAA AGGTTGTTTG TAAAATGAGT 
GACCAGTCTT CACTGGTTCT GGAAGAGTAT TGGGCAACTC TGCAAGAATC CACTTTCAGC 
AAACTGGTCC AGATGTTTAA AACAGCCGTC ATATGCCAGT TGGATTACTG GGATGAAAGT 
GCTGAGGAGA ATGGTAATGT TCAAGCTCTC CTAGAAATGT TGAAGAAGCT GCACAGGGTA 
AACCAGGTGA AATGTCAACT ACCTGAAAGT ATTTTCCAAG TAGACGAACT CTTGCACCGT 
CTCAATTTTT TTGTAGAAGT ATGCAGAAGG TACTTGTGGA AAATGACTGT GGACGCTTCA 
GAAAATGTAC AATGCTGCGT CATATTCAGT CACTTTCCAT TTATCTTTAA TAATCTGTCG 
AAAATTAAAC TACTACATAC AGACACACTT TTAAAAATAG AGAGTAAAAA ACATAAAGCT 
TATCTTAGGT CGGCAGCAAT TGAGGAAGAA AGAGAGTCTG AATTCGCTTT GAGGCCCACG 
TTTGATCTAA CAGTCAGAAG GAATCACTTG ATTGAGGATG TTTTGAATCA GCTAAGTCAA 
TTTGAGAATG AAGACCTGAG GAAAGAGTTA TGGGTTTCAT TTAGTGGAGA AATTGGGTAT 
GACCTCGGAG GAGTCAAGAA AGAGTTCTTC TACTGTCTGT TTGCAGAGAT GATCCAGCCG 
GAATATGGGA TGTTCATGTA TCCTGAAGGG GCTTCCTGCA TGTGGTTTCC TGTCAAGCCT 
AAATTTGAGA AGAAAAGATA CTTCTTTTTT GGGGTTCTAT GTGGACTTTC CCTGTTCAAT 
TGCAATGTTG CCAACCTTCC TTTCCCACTG GCACTGTTTA AGAAACTTTT GGACCAAATG 
CCATCATTGG AAGACTTGAA AGAACTCAGT CCTGATTTGG GAAAGAATTT GCAAACACTT 
CTGGATGATG AAGGTGATAA CTTTGAGGAA GTATTTTACA TCCATTTTAA TGTGCACTGG 
GACAGAAACG ACACAAACTT AATTCCTAAT GGAAGTAGCA TAACTGTCAA CCAGACTAAC 
AAGAGAGACT ATGTTTCTAA GTATATCAAT TACATTTTCA ACGACTCTGT AAAGGCGGTT 
TATGAAGAAT TTCGGAGAGG ATTTTATAAA ATGTGCGACG AAGACATTAT CAAATTATTC 
CACCCCGAAG AACTGAAGGA TGTGATTGTT GGAAATACAG ATTATGATTG GAAAACATTT 
GAAAAGAATG CACGTTATGA ACCAGGATAT AACAGTTCAC ATCCCACCAT AGTGATGTTT 
TGGAAGGCTT TCCACAAATT GACTCTGGAA GAAAAGAAAA AATTCCTTGT ATTTCTTACA 
GGAACTGACA GACTACAAAT GAAAGATTTA AATAATATGA AAATAACATT TTGCTGTCCT 
GAAAGTTGGA ATGAAAGAGA CCCTATAAGA GCACTGACAT GTTTCAGTGT CCTCTTCCTC 
CCTAAATATT CTACAATGGA AACAGTTGAA GAAGCGCTTC AAGAAGCCAT CAACAACAAC 
AGAGGATTTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057407.2
CDS	154..3228
Translation	MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG

Target ORF information:

RefSeq Version	NM_016323.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016323.3

ORF Insert Sequence:

ATGGAGCGGA GGTCGCGGAG GAAGTCGCGG CGCAACGGGC GCTCGACCGC GGGCAAGGCC 
GCCGCGACCC AGCCCGCGAA GTCTCCGGGC GCACAGCTCT GGCTCTTTCC CAGCGCCGCG 
GGCCTCCACC GCGCGCTGCT CCGGAGGGTG GAGGTGACGC GCCAACTCTG CTGCTCGCCG 
GGGCGCCTCG CGGTCTTGGA ACGCGGCGGG GCGGGCGTCC AGGTTCACCA GCTGCTCGCC 
GGGAGCGGCG GCGCCCGGAC GCCGAAATGC ATTAAATTAG GAAAAAACAT GAAGATACAT 
TCCGTGGACC AAGGAGCAGA GCACATGCTG ATTCTCTCAT CAGATGGAAA ACCATTTGAG 
TATGACAACT ATAGCATGAA ACATCTAAGG TTTGAAAGCA TTTTACAAGA AAAAAAAATA 
ATTCAGATCA CATGTGGAGA TTACCATTCT CTTGCACTCT CAAAAGGTGG TGAGCTTTTT 
GCCTGGGGAC AGAACCTGCA TGGGCAGCTT GGAGTTGGAA GGAAATTTCC CTCAACCACC 
ACACCACAGA TTGTGGAGCA CCTCGCAGGA GTACCCTTGG CTCAGATTTC TGCCGGAGAA 
GCCCACAGCA TGGCCTTATC CATGTCTGGC AACATTTATT CATGGGGAAA AAATGAATGT 
GGACAACTAG GCCTGGGCCA CACTGAGAGT AAAGATGATC CATCCCTTAT TGAAGGACTA 
GACAATCAGA AAGTTGAATT TGTCGCTTGT GGTGGCTCTC ACAGTGCCCT ACTCACACAG 
GATGGGCTGC TGTTTACTTT CGGTGCTGGA AAACATGGGC AACTTGGTCA TAATTCAACA 
CAGAATGAGC TAAGACCCTG TTTGGTGGCT GAGCTTGTTG GGTATAGAGT GACTCAGATA 
GCATGTGGAA GGTGGCACAC ACTTGCCTAT GTTTCTGATT TGGGAAAGGT CTTTTCCTTT 
GGTTCTGGAA AAGATGGACA ACTGGGAAAT GGTGGAACAC GTGACCAGCT GATGCCGCTT 
CCAGTGAAAG TATCATCAAG TGAAGAACTC AAACTTGAAA GCCATACCTC AGAAAAGGAG 
TTAATAATGA TTGCTGGAGG GAATCAAAGC ATTTTGCTCT GGATAAAGAA AGAGAATTCA 
TATGTTAATC TGAAGAGGAC AATTCCTACT CTGAATGAAG GGACTGTAAA GAGATGGATT 
GCTGATGTGG AGACTAAACG GTGGCAGAGC ACAAAAAGGG AAATCCAAGA GATATTTTCA 
TCTCCTGCTT GTCTAACTGG AAGTTTTTTA AGGAAAAGAA GAACTACAGA AATGATGCCT 
GTTTATTTGG ACTTAAATAA AGCAAGAAAC ATCTTCAAGG AGTTAACCCA AAAGGACTGG 
ATTACTAACA TGATAACCAC CTGCCTCAAA GATAATCTGC TCAAAAGACT TCCATTTCAT 
TCTCCACCCC AAGAAGCTTT AGAAATTTTC TTCCTTCTCC CAGAATGTCC TATGATGCAT 
ATTTCCAACA ACTGGGAGAG CCTTGTGGTT CCATTTGCAA AGGTTGTTTG TAAAATGAGT 
GACCAGTCTT CACTGGTTCT GGAAGAGTAT TGGGCAACTC TGCAAGAATC CACTTTCAGC 
AAACTGGTCC AGATGTTTAA AACAGCCGTC ATATGCCAGT TGGATTACTG GGATGAAAGT 
GCTGAGGAGA ATGGTAATGT TCAAGCTCTC CTAGAAATGT TGAAGAAGCT GCACAGGGTA 
AACCAGGTGA AATGTCAACT ACCTGAAAGT ATTTTCCAAG TAGACGAACT CTTGCACCGT 
CTCAATTTTT TTGTAGAAGT ATGCAGAAGG TACTTGTGGA AAATGACTGT GGACGCTTCA 
GAAAATGTAC AATGCTGCGT CATATTCAGT CACTTTCCAT TTATCTTTAA TAATCTGTCG 
AAAATTAAAC TACTACATAC AGACACACTT TTAAAAATAG AGAGTAAAAA ACATAAAGCT 
TATCTTAGGT CGGCAGCAAT TGAGGAAGAA AGAGAGTCTG AATTCGCTTT GAGGCCCACG 
TTTGATCTAA CAGTCAGAAG GAATCACTTG ATTGAGGATG TTTTGAATCA GCTAAGTCAA 
TTTGAGAATG AAGACCTGAG GAAAGAGTTA TGGGTTTCAT TTAGTGGAGA AATTGGGTAT 
GACCTCGGAG GAGTCAAGAA AGAGTTCTTC TACTGTCTGT TTGCAGAGAT GATCCAGCCG 
GAATATGGGA TGTTCATGTA TCCTGAAGGG GCTTCCTGCA TGTGGTTTCC TGTCAAGCCT 
AAATTTGAGA AGAAAAGATA CTTCTTTTTT GGGGTTCTAT GTGGACTTTC CCTGTTCAAT 
TGCAATGTTG CCAACCTTCC TTTCCCACTG GCACTGTTTA AGAAACTTTT GGACCAAATG 
CCATCATTGG AAGACTTGAA AGAACTCAGT CCTGATTTGG GAAAGAATTT GCAAACACTT 
CTGGATGATG AAGGTGATAA CTTTGAGGAA GTATTTTACA TCCATTTTAA TGTGCACTGG 
GACAGAAACG ACACAAACTT AATTCCTAAT GGAAGTAGCA TAACTGTCAA CCAGACTAAC 
AAGAGAGACT ATGTTTCTAA GTATATCAAT TACATTTTCA ACGACTCTGT AAAGGCGGTT 
TATGAAGAAT TTCGGAGAGG ATTTTATAAA ATGTGCGACG AAGACATTAT CAAATTATTC 
CACCCCGAAG AACTGAAGGA TGTGATTGTT GGAAATACAG ATTATGATTG GAAAACATTT 
GAAAAGAATG CACGTTATGA ACCAGGATAT AACAGTTCAC ATCCCACCAT AGTGATGTTT 
TGGAAGGCTT TCCACAAATT GACTCTGGAA GAAAAGAAAA AATTCCTTGT ATTTCTTACA 
GGAACTGACA GACTACAAAT GAAAGATTTA AATAATATGA AAATAACATT TTGCTGTCCT 
GAAAGTTGGA ATGAAAGAGA CCCTATAAGA GCACTGACAT GTTTCAGTGT CCTCTTCCTC 
CCTAAATATT CTACAATGGA AACAGTTGAA GAAGCGCTTC AAGAAGCCAT CAACAACAAC 
AGAGGATTTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC5 ( NM_016323.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057407.2 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.

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CloneID	OHu00347
Clone ID Related Accession (Same CDS sequence)	NM_016323.3 , NM_016323.4
Accession Version	NM_016323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	E3 ISG15--protein ligase HERC5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY337518.1, BC140716.1, AK302142.1, AC083829.5 and AI336946.1. On Nov 22, 2018 this sequence version replaced NM_016323.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.122484.1, AY337518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264350.8/ ENSP00000264350.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCGGA GGTCGCGGAG GAAGTCGCGG CGCAACGGGC GCTCGACCGC GGGCAAGGCC 
GCCGCGACCC AGCCCGCGAA GTCTCCGGGC GCACAGCTCT GGCTCTTTCC CAGCGCCGCG 
GGCCTCCACC GCGCGCTGCT CCGGAGGGTG GAGGTGACGC GCCAACTCTG CTGCTCGCCG 
GGGCGCCTCG CGGTCTTGGA ACGCGGCGGG GCGGGCGTCC AGGTTCACCA GCTGCTCGCC 
GGGAGCGGCG GCGCCCGGAC GCCGAAATGC ATTAAATTAG GAAAAAACAT GAAGATACAT 
TCCGTGGACC AAGGAGCAGA GCACATGCTG ATTCTCTCAT CAGATGGAAA ACCATTTGAG 
TATGACAACT ATAGCATGAA ACATCTAAGG TTTGAAAGCA TTTTACAAGA AAAAAAAATA 
ATTCAGATCA CATGTGGAGA TTACCATTCT CTTGCACTCT CAAAAGGTGG TGAGCTTTTT 
GCCTGGGGAC AGAACCTGCA TGGGCAGCTT GGAGTTGGAA GGAAATTTCC CTCAACCACC 
ACACCACAGA TTGTGGAGCA CCTCGCAGGA GTACCCTTGG CTCAGATTTC TGCCGGAGAA 
GCCCACAGCA TGGCCTTATC CATGTCTGGC AACATTTATT CATGGGGAAA AAATGAATGT 
GGACAACTAG GCCTGGGCCA CACTGAGAGT AAAGATGATC CATCCCTTAT TGAAGGACTA 
GACAATCAGA AAGTTGAATT TGTCGCTTGT GGTGGCTCTC ACAGTGCCCT ACTCACACAG 
GATGGGCTGC TGTTTACTTT CGGTGCTGGA AAACATGGGC AACTTGGTCA TAATTCAACA 
CAGAATGAGC TAAGACCCTG TTTGGTGGCT GAGCTTGTTG GGTATAGAGT GACTCAGATA 
GCATGTGGAA GGTGGCACAC ACTTGCCTAT GTTTCTGATT TGGGAAAGGT CTTTTCCTTT 
GGTTCTGGAA AAGATGGACA ACTGGGAAAT GGTGGAACAC GTGACCAGCT GATGCCGCTT 
CCAGTGAAAG TATCATCAAG TGAAGAACTC AAACTTGAAA GCCATACCTC AGAAAAGGAG 
TTAATAATGA TTGCTGGAGG GAATCAAAGC ATTTTGCTCT GGATAAAGAA AGAGAATTCA 
TATGTTAATC TGAAGAGGAC AATTCCTACT CTGAATGAAG GGACTGTAAA GAGATGGATT 
GCTGATGTGG AGACTAAACG GTGGCAGAGC ACAAAAAGGG AAATCCAAGA GATATTTTCA 
TCTCCTGCTT GTCTAACTGG AAGTTTTTTA AGGAAAAGAA GAACTACAGA AATGATGCCT 
GTTTATTTGG ACTTAAATAA AGCAAGAAAC ATCTTCAAGG AGTTAACCCA AAAGGACTGG 
ATTACTAACA TGATAACCAC CTGCCTCAAA GATAATCTGC TCAAAAGACT TCCATTTCAT 
TCTCCACCCC AAGAAGCTTT AGAAATTTTC TTCCTTCTCC CAGAATGTCC TATGATGCAT 
ATTTCCAACA ACTGGGAGAG CCTTGTGGTT CCATTTGCAA AGGTTGTTTG TAAAATGAGT 
GACCAGTCTT CACTGGTTCT GGAAGAGTAT TGGGCAACTC TGCAAGAATC CACTTTCAGC 
AAACTGGTCC AGATGTTTAA AACAGCCGTC ATATGCCAGT TGGATTACTG GGATGAAAGT 
GCTGAGGAGA ATGGTAATGT TCAAGCTCTC CTAGAAATGT TGAAGAAGCT GCACAGGGTA 
AACCAGGTGA AATGTCAACT ACCTGAAAGT ATTTTCCAAG TAGACGAACT CTTGCACCGT 
CTCAATTTTT TTGTAGAAGT ATGCAGAAGG TACTTGTGGA AAATGACTGT GGACGCTTCA 
GAAAATGTAC AATGCTGCGT CATATTCAGT CACTTTCCAT TTATCTTTAA TAATCTGTCG 
AAAATTAAAC TACTACATAC AGACACACTT TTAAAAATAG AGAGTAAAAA ACATAAAGCT 
TATCTTAGGT CGGCAGCAAT TGAGGAAGAA AGAGAGTCTG AATTCGCTTT GAGGCCCACG 
TTTGATCTAA CAGTCAGAAG GAATCACTTG ATTGAGGATG TTTTGAATCA GCTAAGTCAA 
TTTGAGAATG AAGACCTGAG GAAAGAGTTA TGGGTTTCAT TTAGTGGAGA AATTGGGTAT 
GACCTCGGAG GAGTCAAGAA AGAGTTCTTC TACTGTCTGT TTGCAGAGAT GATCCAGCCG 
GAATATGGGA TGTTCATGTA TCCTGAAGGG GCTTCCTGCA TGTGGTTTCC TGTCAAGCCT 
AAATTTGAGA AGAAAAGATA CTTCTTTTTT GGGGTTCTAT GTGGACTTTC CCTGTTCAAT 
TGCAATGTTG CCAACCTTCC TTTCCCACTG GCACTGTTTA AGAAACTTTT GGACCAAATG 
CCATCATTGG AAGACTTGAA AGAACTCAGT CCTGATTTGG GAAAGAATTT GCAAACACTT 
CTGGATGATG AAGGTGATAA CTTTGAGGAA GTATTTTACA TCCATTTTAA TGTGCACTGG 
GACAGAAACG ACACAAACTT AATTCCTAAT GGAAGTAGCA TAACTGTCAA CCAGACTAAC 
AAGAGAGACT ATGTTTCTAA GTATATCAAT TACATTTTCA ACGACTCTGT AAAGGCGGTT 
TATGAAGAAT TTCGGAGAGG ATTTTATAAA ATGTGCGACG AAGACATTAT CAAATTATTC 
CACCCCGAAG AACTGAAGGA TGTGATTGTT GGAAATACAG ATTATGATTG GAAAACATTT 
GAAAAGAATG CACGTTATGA ACCAGGATAT AACAGTTCAC ATCCCACCAT AGTGATGTTT 
TGGAAGGCTT TCCACAAATT GACTCTGGAA GAAAAGAAAA AATTCCTTGT ATTTCTTACA 
GGAACTGACA GACTACAAAT GAAAGATTTA AATAATATGA AAATAACATT TTGCTGTCCT 
GAAAGTTGGA ATGAAAGAGA CCCTATAAGA GCACTGACAT GTTTCAGTGT CCTCTTCCTC 
CCTAAATATT CTACAATGGA AACAGTTGAA GAAGCGCTTC AAGAAGCCAT CAACAACAAC 
AGAGGATTTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057407.2
CDS	152..3226
Translation	MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG

Target ORF information:

RefSeq Version	NM_016323.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 5 (HERC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016323.4

ORF Insert Sequence:

ATGGAGCGGA GGTCGCGGAG GAAGTCGCGG CGCAACGGGC GCTCGACCGC GGGCAAGGCC 
GCCGCGACCC AGCCCGCGAA GTCTCCGGGC GCACAGCTCT GGCTCTTTCC CAGCGCCGCG 
GGCCTCCACC GCGCGCTGCT CCGGAGGGTG GAGGTGACGC GCCAACTCTG CTGCTCGCCG 
GGGCGCCTCG CGGTCTTGGA ACGCGGCGGG GCGGGCGTCC AGGTTCACCA GCTGCTCGCC 
GGGAGCGGCG GCGCCCGGAC GCCGAAATGC ATTAAATTAG GAAAAAACAT GAAGATACAT 
TCCGTGGACC AAGGAGCAGA GCACATGCTG ATTCTCTCAT CAGATGGAAA ACCATTTGAG 
TATGACAACT ATAGCATGAA ACATCTAAGG TTTGAAAGCA TTTTACAAGA AAAAAAAATA 
ATTCAGATCA CATGTGGAGA TTACCATTCT CTTGCACTCT CAAAAGGTGG TGAGCTTTTT 
GCCTGGGGAC AGAACCTGCA TGGGCAGCTT GGAGTTGGAA GGAAATTTCC CTCAACCACC 
ACACCACAGA TTGTGGAGCA CCTCGCAGGA GTACCCTTGG CTCAGATTTC TGCCGGAGAA 
GCCCACAGCA TGGCCTTATC CATGTCTGGC AACATTTATT CATGGGGAAA AAATGAATGT 
GGACAACTAG GCCTGGGCCA CACTGAGAGT AAAGATGATC CATCCCTTAT TGAAGGACTA 
GACAATCAGA AAGTTGAATT TGTCGCTTGT GGTGGCTCTC ACAGTGCCCT ACTCACACAG 
GATGGGCTGC TGTTTACTTT CGGTGCTGGA AAACATGGGC AACTTGGTCA TAATTCAACA 
CAGAATGAGC TAAGACCCTG TTTGGTGGCT GAGCTTGTTG GGTATAGAGT GACTCAGATA 
GCATGTGGAA GGTGGCACAC ACTTGCCTAT GTTTCTGATT TGGGAAAGGT CTTTTCCTTT 
GGTTCTGGAA AAGATGGACA ACTGGGAAAT GGTGGAACAC GTGACCAGCT GATGCCGCTT 
CCAGTGAAAG TATCATCAAG TGAAGAACTC AAACTTGAAA GCCATACCTC AGAAAAGGAG 
TTAATAATGA TTGCTGGAGG GAATCAAAGC ATTTTGCTCT GGATAAAGAA AGAGAATTCA 
TATGTTAATC TGAAGAGGAC AATTCCTACT CTGAATGAAG GGACTGTAAA GAGATGGATT 
GCTGATGTGG AGACTAAACG GTGGCAGAGC ACAAAAAGGG AAATCCAAGA GATATTTTCA 
TCTCCTGCTT GTCTAACTGG AAGTTTTTTA AGGAAAAGAA GAACTACAGA AATGATGCCT 
GTTTATTTGG ACTTAAATAA AGCAAGAAAC ATCTTCAAGG AGTTAACCCA AAAGGACTGG 
ATTACTAACA TGATAACCAC CTGCCTCAAA GATAATCTGC TCAAAAGACT TCCATTTCAT 
TCTCCACCCC AAGAAGCTTT AGAAATTTTC TTCCTTCTCC CAGAATGTCC TATGATGCAT 
ATTTCCAACA ACTGGGAGAG CCTTGTGGTT CCATTTGCAA AGGTTGTTTG TAAAATGAGT 
GACCAGTCTT CACTGGTTCT GGAAGAGTAT TGGGCAACTC TGCAAGAATC CACTTTCAGC 
AAACTGGTCC AGATGTTTAA AACAGCCGTC ATATGCCAGT TGGATTACTG GGATGAAAGT 
GCTGAGGAGA ATGGTAATGT TCAAGCTCTC CTAGAAATGT TGAAGAAGCT GCACAGGGTA 
AACCAGGTGA AATGTCAACT ACCTGAAAGT ATTTTCCAAG TAGACGAACT CTTGCACCGT 
CTCAATTTTT TTGTAGAAGT ATGCAGAAGG TACTTGTGGA AAATGACTGT GGACGCTTCA 
GAAAATGTAC AATGCTGCGT CATATTCAGT CACTTTCCAT TTATCTTTAA TAATCTGTCG 
AAAATTAAAC TACTACATAC AGACACACTT TTAAAAATAG AGAGTAAAAA ACATAAAGCT 
TATCTTAGGT CGGCAGCAAT TGAGGAAGAA AGAGAGTCTG AATTCGCTTT GAGGCCCACG 
TTTGATCTAA CAGTCAGAAG GAATCACTTG ATTGAGGATG TTTTGAATCA GCTAAGTCAA 
TTTGAGAATG AAGACCTGAG GAAAGAGTTA TGGGTTTCAT TTAGTGGAGA AATTGGGTAT 
GACCTCGGAG GAGTCAAGAA AGAGTTCTTC TACTGTCTGT TTGCAGAGAT GATCCAGCCG 
GAATATGGGA TGTTCATGTA TCCTGAAGGG GCTTCCTGCA TGTGGTTTCC TGTCAAGCCT 
AAATTTGAGA AGAAAAGATA CTTCTTTTTT GGGGTTCTAT GTGGACTTTC CCTGTTCAAT 
TGCAATGTTG CCAACCTTCC TTTCCCACTG GCACTGTTTA AGAAACTTTT GGACCAAATG 
CCATCATTGG AAGACTTGAA AGAACTCAGT CCTGATTTGG GAAAGAATTT GCAAACACTT 
CTGGATGATG AAGGTGATAA CTTTGAGGAA GTATTTTACA TCCATTTTAA TGTGCACTGG 
GACAGAAACG ACACAAACTT AATTCCTAAT GGAAGTAGCA TAACTGTCAA CCAGACTAAC 
AAGAGAGACT ATGTTTCTAA GTATATCAAT TACATTTTCA ACGACTCTGT AAAGGCGGTT 
TATGAAGAAT TTCGGAGAGG ATTTTATAAA ATGTGCGACG AAGACATTAT CAAATTATTC 
CACCCCGAAG AACTGAAGGA TGTGATTGTT GGAAATACAG ATTATGATTG GAAAACATTT 
GAAAAGAATG CACGTTATGA ACCAGGATAT AACAGTTCAC ATCCCACCAT AGTGATGTTT 
TGGAAGGCTT TCCACAAATT GACTCTGGAA GAAAAGAAAA AATTCCTTGT ATTTCTTACA 
GGAACTGACA GACTACAAAT GAAAGATTTA AATAATATGA AAATAACATT TTGCTGTCCT 
GAAAGTTGGA ATGAAAGAGA CCCTATAAGA GCACTGACAT GTTTCAGTGT CCTCTTCCTC 
CCTAAATATT CTACAATGGA AACAGTTGAA GAAGCGCTTC AAGAAGCCAT CAACAACAAC 
AGAGGATTTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HECT Domain and RCC1-Like Domain-Containing Protein 5 (HERC-5) Gene Polymorphisms in HIV-1-Infected Individuals: A Study from India.
AIDS research and human retroviruses33(11)1171-1174(2017 Nov)
Shaikh N,Saxena V,Pandey S,Patil A,Thakar M

Covalent ISG15 conjugation positively regulates the ubiquitin E3 ligase activity of parkin.
Open biology6(8)(2016 08)
Im E,Yoo L,Hyun M,Shin WH,Chung KC

HERC5 is a prognostic biomarker for post-liver transplant recurrent human hepatocellular carcinoma.
Journal of translational medicine13379(2015 Dec)
Xue F,Higgs BW,Huang J,Morehouse C,Zhu W,Yao X,Brohawn P,Xiao Z,Sebastian Y,Liu Z,Xia Y,Shen D,Kuziora M,Dong Z,Han H,Gu Y,Gu J,Xia Q,Yao Y

Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase.
The Journal of general virology96(11)3236-42(2015 Nov)
Domingues P,Bamford CG,Boutell C,McLauchlan J

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HERC5 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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