NAGPA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAGPA
Entrez Gene ID	51172
Full Name	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Synonyms	APAA,UCE
General protein information	Preferred Names N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase Names N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase alpha-N-acetylglucosaminyl phosphodiesterase lysosomal alpha-N-acetylglucosaminidase mannose 6-phosphate-uncovering enzyme phosphodiester alpha-GlcNAcase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015].
Disorder MIM:	607985

mRNA and Protein(s)

mRNA	Protein	Name
XM_011522517.3	XP_011520819.1	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase isoform X1
NM_016256.4	NP_057340.2	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase precursor
NM_016256.3	NP_057340.2	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase precursor
XM_011522517.2	XP_011520819.1	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nagpa	NP_001101735.1
Danio rerio (zebrafish)	zgc:165534	NP_001103185.1
Homo sapiens (human)	NAGPA	NP_057340.2
Pan troglodytes (chimpanzee)	NAGPA	XP_510795.2
	NAGPA	XP_001100122.1
Canis lupus familiaris (dog)	NAGPA	XP_005621636.1
Mus musculus (house mouse)	Nagpa	NP_038824.2
Gallus gallus (chicken)	NAGPA	XP_414709.4
Xenopus tropicalis (tropical clawed frog)	nagpa	XP_002942191.2
Bos taurus (cattle)	NAGPA	NP_001193547.1

Gene Ontology
Bibliography

Related articles in PubMed

Variants in GNPTAB, GNPTG and NAGPA genes are associated with stutterers.
Kazemi N, Estiar MA, Fazilaty H, Sakhinia E
Gene(2017 Dec)

Mucolipidosis types II and III and non-syndromic stuttering are associated with different variants in the same genes.
Raza MH, Domingues CE, Webster R, Sainz E, Paris E, Rahn R, Gutierrez J, Chow HM, Mundorff J, Kang CS, Riaz N, Basra MA, Khan S, Riazuddin S, Moretti-Ferreira D, Braun A, Drayna D
European journal of human genetics : EJHG24(4)529-34(2016 Apr)

Association between Rare Variants in AP4E1, a Component of Intracellular Trafficking, and Persistent Stuttering.
Raza MH, Mattera R, Morell R, Sainz E, Rahn R, Gutierrez J, Paris E, Root J, Solomon B, Brewer C, Basra MA, Khan S, Riazuddin S, Braun A, Bonifacino JS, Drayna D
American journal of human genetics97(5)715-25(2015 Nov)

Association study of stuttering candidate genes GNPTAB, GNPTG and NAGPA with dyslexia in Chinese population.
Chen H, Xu J, Zhou Y, Gao Y, Wang G, Xia J, Huen MS, Siok WT, Jiang Y, Tan LH, Sun Y
BMC genetics167(2015 Feb)

Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.
Das D, Lee WS, Grant JC, Chiu HJ, Farr CL, Vance J, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Kornfeld S, Wilson IA
The Journal of biological chemistry288(23)16789-99(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SNPs covering GNPTAB, GNPTG and NAGPA were subjected to genotyping, association analysis was performed on all SNPs. Significant association of rs17031962 in GNPTAB and rs882294 in NAGPA with developmental dyslexia in a Chinese population was identified after false discovery rate correction for multiple comparisons.
Title: Association study of stuttering candidate genes GNPTAB, GNPTG and NAGPA with dyslexia in Chinese population.

Mutational analysis of several residues in a highly conserved surface cavity of hUCE revealed that they are essential for function.
Title: Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.

To date mutations in GNPTAB, GNPTG, and NAGPA have been associated with stuttering. These genes encode the lysosomal enzyme targeting pathway, defective in mucolipidosis. (Review)
Title: A role for inherited metabolic deficits in persistent developmental stuttering.

Analysis of mannose 6-phosphate uncovering enzyme mutations associated with persistent stuttering.
Title: Analysis of mannose 6-phosphate uncovering enzyme mutations associated with persistent stuttering.

identified three mutations in the NAGPA gene associated with stuttering
Title: Mutations in the lysosomal enzyme-targeting pathway and persistent stuttering.

The following NAGPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAGPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21223	NM_016256.4 Latest version!	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu67734	XM_011522517.3 Latest version!	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu21223	NM_016256.3	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu67734	XM_011522517.2	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAGPA ( NM_016256.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057340.2 Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

Price & Availability↑

CloneID	OHu21223
Clone ID Related Accession (Same CDS sequence)	NM_016256.4 , NM_016256.3
Accession Version	NM_016256.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA879815.1, AK025570.1 and AC026458.8. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_016256.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK127952.1, AF187072.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312251.8/ ENSP00000310998.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG GACCGCCTGG CTAGCCCTCA CCCTGGCGCT GGCCTTCCTC 
CTGCTGATCA GCACTGCAGC AAACCTGTCC TTGCTCCTGT CCAGAGCAGA GAGGAACCGG 
CGCCTGCATG GGGACTATGC ATACCACCCG CTGCAGGAGA TGAACGGGGA GCCTCTGGCC 
GCAGAGAAGG AGCAGCCAGG GGGCGCCCAC AACCCCTTCA AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057340.2
CDS	22..1569
Translation	MATSTGRWLLLRLALFGFLWEASGGLDSGASRDDDLLLPYPRARARLPRDCTRVRAGNREHESWPPPPATPGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGFFRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCHGHGTCVDGHCQCTGHFWRGPGCDELDCGPSNCSQHGLCTETGCRCDAGWTGSNCSEECPLGWHGPGCQRPCKCEHHCPCDPKTGNCSVSRVKQCLQPPEATLRAGELSFFTRTAWLALTLALAFLLLISTAANLSLLLSRAERNRRLHGDYAYHPLQEMNGEPLAAEKEQPGGAHNPFKD

Target ORF information:

RefSeq Version	NM_016256.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016256.4

ORF Insert Sequence:

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG GACCGCCTGG CTAGCCCTCA CCCTGGCGCT GGCCTTCCTC 
CTGCTGATCA GCACTGCAGC AAACCTGTCC TTGCTCCTGT CCAGAGCAGA GAGGAACCGG 
CGCCTGCATG GGGACTATGC ATACCACCCG CTGCAGGAGA TGAACGGGGA GCCTCTGGCC 
GCAGAGAAGG AGCAGCCAGG GGGCGCCCAC AACCCCTTCA AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAGPA ( NM_016256.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057340.2 Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

Price & Availability↑

CloneID	OHu21223
Clone ID Related Accession (Same CDS sequence)	NM_016256.4 , NM_016256.3
Accession Version	NM_016256.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA879815.1, AK025570.1 and AC026458.8. This sequence is a reference standard in the RefSeqGene project. On Jul 31, 2010 this sequence version replaced NM_016256.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK025570.1, AF187072.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG GACCGCCTGG CTAGCCCTCA CCCTGGCGCT GGCCTTCCTC 
CTGCTGATCA GCACTGCAGC AAACCTGTCC TTGCTCCTGT CCAGAGCAGA GAGGAACCGG 
CGCCTGCATG GGGACTATGC ATACCACCCG CTGCAGGAGA TGAACGGGGA GCCTCTGGCC 
GCAGAGAAGG AGCAGCCAGG GGGCGCCCAC AACCCCTTCA AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057340.2
CDS	28..1575
Translation	MATSTGRWLLLRLALFGFLWEASGGLDSGASRDDDLLLPYPRARARLPRDCTRVRAGNREHESWPPPPATPGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGFFRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCHGHGTCVDGHCQCTGHFWRGPGCDELDCGPSNCSQHGLCTETGCRCDAGWTGSNCSEECPLGWHGPGCQRPCKCEHHCPCDPKTGNCSVSRVKQCLQPPEATLRAGELSFFTRTAWLALTLALAFLLLISTAANLSLLLSRAERNRRLHGDYAYHPLQEMNGEPLAAEKEQPGGAHNPFKD

Target ORF information:

RefSeq Version	NM_016256.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016256.3

ORF Insert Sequence:

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG GACCGCCTGG CTAGCCCTCA CCCTGGCGCT GGCCTTCCTC 
CTGCTGATCA GCACTGCAGC AAACCTGTCC TTGCTCCTGT CCAGAGCAGA GAGGAACCGG 
CGCCTGCATG GGGACTATGC ATACCACCCG CTGCAGGAGA TGAACGGGGA GCCTCTGGCC 
GCAGAGAAGG AGCAGCCAGG GGGCGCCCAC AACCCCTTCA AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAGPA ( XM_011522517.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520819.1 Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67734
Clone ID Related Accession (Same CDS sequence)	XM_011522517.3 , XM_011522517.2
Accession Version	XM_011522517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011522517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG AGCAGAGAGG AACCGGCGCC TGCATGGGGA CTATGCATAC 
CACCCGCTGC AGGAGATGAA CGGGGAGCCT CTGGCCGCAG AGAAGGAGCA GCCAGGGGGC 
GCCCACAACC CCTTCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520819.1
CDS	27..1490
Translation	MATSTGRWLLLRLALFGFLWEASGGLDSGASRDDDLLLPYPRARARLPRDCTRVRAGNREHESWPPPPATPGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGFFRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCHGHGTCVDGHCQCTGHFWRGPGCDELDCGPSNCSQHGLCTETGCRCDAGWTGSNCSEECPLGWHGPGCQRPCKCEHHCPCDPKTGNCSVSRVKQCLQPPEATLRAGELSFFTRAERNRRLHGDYAYHPLQEMNGEPLAAEKEQPGGAHNPFKD

Target ORF information:

RefSeq Version	XM_011522517.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522517.3

ORF Insert Sequence:

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG AGCAGAGAGG AACCGGCGCC TGCATGGGGA CTATGCATAC 
CACCCGCTGC AGGAGATGAA CGGGGAGCCT CTGGCCGCAG AGAAGGAGCA GCCAGGGGGC 
GCCCACAACC CCTTCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAGPA ( XM_011522517.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520819.1 Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.

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CloneID	OHu67734
Clone ID Related Accession (Same CDS sequence)	XM_011522517.3 , XM_011522517.2
Accession Version	XM_011522517.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG AGCAGAGAGG AACCGGCGCC TGCATGGGGA CTATGCATAC 
CACCCGCTGC AGGAGATGAA CGGGGAGCCT CTGGCCGCAG AGAAGGAGCA GCCAGGGGGC 
GCCCACAACC CCTTCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520819.1
CDS	28..1491
Translation	MATSTGRWLLLRLALFGFLWEASGGLDSGASRDDDLLLPYPRARARLPRDCTRVRAGNREHESWPPPPATPGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGFFRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCHGHGTCVDGHCQCTGHFWRGPGCDELDCGPSNCSQHGLCTETGCRCDAGWTGSNCSEECPLGWHGPGCQRPCKCEHHCPCDPKTGNCSVSRVKQCLQPPEATLRAGELSFFTRAERNRRLHGDYAYHPLQEMNGEPLAAEKEQPGGAHNPFKD

Target ORF information:

RefSeq Version	XM_011522517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522517.2

ORF Insert Sequence:

ATGGCGACCT CCACGGGTCG CTGGCTTCTC CTCCGGCTTG CACTATTCGG CTTCCTCTGG 
GAAGCGTCCG GCGGCCTCGA CTCGGGGGCC TCCCGCGACG ACGACTTGCT ACTGCCCTAT 
CCACGCGCGC GCGCGCGCCT CCCCCGGGAC TGCACACGGG TGCGCGCCGG CAACCGCGAG 
CACGAGAGTT GGCCTCCGCC TCCCGCGACT CCCGGCGCCG GCGGTCTGGC CGTGCGCACC 
TTCGTGTCGC ACTTCAGGGA CCGCGCGGTG GCCGGCCACC TGACGCGGGC CGTTGAGCCC 
CTGCGCACCT TCTCGGTGCT GGAGCCCGGT GGACCCGGCG GCTGCGCGGC GAGACGACGC 
GCCACCGTGG AGGAGACGGC GCGGGCGGCC GACTGCCGTG TCGCCCAGAA CGGCGGCTTC 
TTCCGCATGA ACTCGGGCGA GTGCCTGGGG AACGTGGTGA GCGACGAGCG GCGGGTGAGC 
AGCTCCGGGG GGCTGCAGAA CGCGCAGTTC GGGATCCGCC GCGACGGGAC CCTGGTCACC 
GGGTACCTGT CTGAGGAGGA GGTGCTGGAC ACTGAGAACC CATTTGTGCA GCTGCTGAGT 
GGGGTCGTGT GGCTGATTCG TAATGGAAGC ATCTACATCA ACGAGAGCCA AGCCACAGAG 
TGTGACGAGA CACAGGAGAC AGGTTCCTTT AGCAAATTTG TGAATGTGAT ATCAGCCAGG 
ACGGCCATTG GCCACGACCG GAAAGGGCAG CTGGTGCTCT TTCATGCAGA CGGCCAAACG 
GAGCAGCGTG GCATCAACCT GTGGGAAATG GCGGAGTTCC TGCTGAAACA GGACGTGGTC 
AACGCCATCA ACCTGGATGG GGGTGGCTCT GCCACCTTTG TGCTCAACGG GACCTTGGCC 
AGTTACCCGT CAGATCACTG CCAGGACAAC ATGTGGCGCT GTCCCCGCCA AGTGTCCACC 
GTGGTGTGTG TGCACGAACC CCGCTGCCAG CCGCCTGACT GCCACGGCCA CGGGACCTGC 
GTGGACGGGC ACTGCCAATG CACCGGGCAC TTCTGGCGGG GTCCCGGCTG TGATGAGCTG 
GACTGTGGCC CCTCTAACTG CAGCCAGCAC GGACTGTGCA CGGAGACCGG CTGCCGCTGT 
GATGCCGGAT GGACCGGGTC CAACTGCAGT GAAGAGTGTC CCCTTGGCTG GCATGGGCCG 
GGCTGCCAGA GGCCTTGTAA GTGTGAGCAC CATTGTCCCT GTGACCCCAA GACTGGCAAC 
TGCAGCGTCT CCAGAGTAAA GCAGTGTCTC CAGCCACCTG AAGCCACCCT GAGGGCGGGA 
GAACTCTCCT TTTTCACCAG AGCAGAGAGG AACCGGCGCC TGCATGGGGA CTATGCATAC 
CACCCGCTGC AGGAGATGAA CGGGGAGCCT CTGGCCGCAG AGAAGGAGCA GCCAGGGGGC 
GCCCACAACC CCTTCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variants in GNPTAB, GNPTG and NAGPA genes are associated with stutterers.
Gene(2017 Dec)
Kazemi N,Estiar MA,Fazilaty H,Sakhinia E

Mucolipidosis types II and III and non-syndromic stuttering are associated with different variants in the same genes.
European journal of human genetics : EJHG24(4)529-34(2016 Apr)
Raza MH,Domingues CE,Webster R,Sainz E,Paris E,Rahn R,Gutierrez J,Chow HM,Mundorff J,Kang CS,Riaz N,Basra MA,Khan S,Riazuddin S,Moretti-Ferreira D,Braun A,Drayna D

Association between Rare Variants in AP4E1, a Component of Intracellular Trafficking, and Persistent Stuttering.
American journal of human genetics97(5)715-25(2015 Nov)
Raza MH,Mattera R,Morell R,Sainz E,Rahn R,Gutierrez J,Paris E,Root J,Solomon B,Brewer C,Basra MA,Khan S,Riazuddin S,Braun A,Bonifacino JS,Drayna D

Association study of stuttering candidate genes GNPTAB, GNPTG and NAGPA with dyslexia in Chinese population.
BMC genetics167(2015 Feb)
Chen H,Xu J,Zhou Y,Gao Y,Wang G,Xia J,Huen MS,Siok WT,Jiang Y,Tan LH,Sun Y

Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.
The Journal of biological chemistry288(23)16789-99(2013 Jun)
Das D,Lee WS,Grant JC,Chiu HJ,Farr CL,Vance J,Klock HE,Knuth MW,Miller MD,Elsliger MA,Deacon AM,Godzik A,Lesley SA,Kornfeld S,Wilson IA

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NAGPA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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