RLIM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RLIM
Entrez Gene ID	51132
Full Name	ring finger protein, LIM domain interacting
Synonyms	MRX61,NY-REN-43,RNF12
General protein information	Preferred Names ring finger protein, LIM domain interacting Names E3 ubiquitin-protein ligase RLIM E3 ubiquitin-protein ligase RNF12 LIM domain-interacting RING finger protein R-LIM RING finger LIM domain-binding protein RING-type E3 ubiquitin transferase RLIM renal carcinoma antigen NY-REN-43 ring finger protein 12 ring zinc finger LIM domain binding protein ring zinc finger protein NY-REN-43antigen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq13.2
Summary	The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009].
Disorder MIM:	300379
Disorder Html:	Mental retardation, X-linked 61, 300978 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016120.4	NP_057204.2	E3 ubiquitin-protein ligase RLIM
NM_183353.2	NP_899196.1	E3 ubiquitin-protein ligase RLIM
NM_016120.3	NP_057204.2	E3 ubiquitin-protein ligase RLIM
NM_183353.3	NP_899196.1	E3 ubiquitin-protein ligase RLIM

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC562487	XP_686027.4
Mus musculus (house mouse)	Rlim	NP_035406.3
Rattus norvegicus (Norway rat)	LOC100910646	XP_006257203.1
Gallus gallus (chicken)	RLIM	NP_990159.1
Xenopus tropicalis (tropical clawed frog)	rlim	NP_001016091.1
Homo sapiens (human)	RLIM	NP_057204.2
Pan troglodytes (chimpanzee)	RLIM	XP_001142065.1
Canis lupus familiaris (dog)	RLIM	XP_005641563.1
Rattus norvegicus (Norway rat)	Rlim	NP_001020063.1
	RLIM	XP_001096367.1

Gene Ontology
Bibliography

Related articles in PubMed

RNF12 promotes p53-dependent cell growth suppression and apoptosis by targeting MDM2 for destruction.
Gao K, Wang C, Jin X, Xiao J, Zhang E, Yang X, Wang D, Huang H, Yu L, Zhang P
Cancer letters375(1)133-141(2016 May)

Syndromic X-linked intellectual disability segregating with a missense variant in RLIM.
T?nne E, Holdhus R, Stansberg C, Stray-Pedersen A, Petersen K, Brunner HG, Gilissen C, Hoischen A, Prescott T, Steen VM, Fiskerstrand T
European journal of human genetics : EJHG23(12)1652-6(2015 Dec)

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrent? L, Mar JC
PLoS genetics11(8)e1005428(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNF12 is a novel positive regulator of p53 pathway and an external E3 ubiquitin ligase for MDM2 destruction.
Title: RNF12 promotes p53-dependent cell growth suppression and apoptosis by targeting MDM2 for destruction.

missense mutation in RLIM gene was identified in four males with intellectual disability from a three-generation family.
Title: Syndromic X-linked intellectual disability segregating with a missense variant in RLIM.

Rlim is a novel regulator of Stathmin protein in a ubiquitin-dependent manner, and represents a new pathway for malignant phenotype turnover by modulating the level of Stathmin protein in human osteosarcomas.
Title: Rlim, an E3 ubiquitin ligase, influences the stability of Stathmin protein in human osteosarcoma cells.

hus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival
Title: Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling.

TP53 does not directly bind to the RLIM promoter, it physically interacts with and prevents the binding of Sp1 to the RLIM promoter.
Title: p53 Represses transcription of RING finger LIM domain-binding protein RLIM through Sp1.

The following RLIM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RLIM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23727	NM_183353.3 Latest version!	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23727	NM_016120.4 Latest version!	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu23727	NM_016120.3	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23727	NM_183353.2	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RLIM ( NM_016120.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057204.2 Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23727
Clone ID Related Accession (Same CDS sequence)	NM_016120.4 , NM_183353.2 , NM_016120.3 , NM_183353.3
Accession Version	NM_016120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RLIM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF686000.1, BC013357.2, AL513007.5 and AK127880.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_016120.3. Transcript Variant: This variant (1) lacks an alternate exon in the 5' UTR, compared to variant 2, and may represent the more abundant form. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.89394.1, SRR1803611.124621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000332687.11/ ENSP00000328059.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057204.2
CDS	227..2101
Translation	MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV

Target ORF information:

RefSeq Version	NM_016120.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016120.4

ORF Insert Sequence:

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RLIM ( NM_183353.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_899196.1 Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23727
Clone ID Related Accession (Same CDS sequence)	NM_016120.4 , NM_183353.2 , NM_016120.3 , NM_183353.3
Accession Version	NM_183353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RLIM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF686000.1, AL712605.1, BC013357.2, AL513007.5 and AK127880.1. On Feb 19, 2009 this sequence version replaced NM_183353.1. Transcript Variant: This variant (2) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.36183.1, SRR1803615.220179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899196.1
CDS	291..2165
Translation	MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV

Target ORF information:

RefSeq Version	NM_183353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183353.2

ORF Insert Sequence:

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RLIM ( NM_016120.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057204.2 Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.

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CloneID	OHu23727
Clone ID Related Accession (Same CDS sequence)	NM_016120.4 , NM_183353.2 , NM_016120.3 , NM_183353.3
Accession Version	NM_016120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RLIM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF686000.1, BC013357.2, AL513007.5 and AK127880.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2009 this sequence version replaced NM_016120.2. Transcript Variant: This variant (1) lacks an alternate exon in the 5' UTR, compared to variant 2, and may represent the more abundant form. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.89394.1, SRR1803612.51186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057204.2
CDS	229..2103
Translation	MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV

Target ORF information:

RefSeq Version	NM_016120.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016120.3

ORF Insert Sequence:

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RLIM ( NM_183353.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_899196.1 Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23727
Clone ID Related Accession (Same CDS sequence)	NM_016120.4 , NM_183353.2 , NM_016120.3 , NM_183353.3
Accession Version	NM_183353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RLIM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF686000.1, AL712605.1, BC013357.2, AL513007.5 and AK127880.1. On May 31, 2019 this sequence version replaced NM_183353.2. Transcript Variant: This variant (2) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.36183.1, SRR1803615.220179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899196.1
CDS	289..2163
Translation	MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV

Target ORF information:

RefSeq Version	NM_183353.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183353.3

ORF Insert Sequence:

ATGGAAAACT CAGATTCCAA TGACAAAGGA AGTGGTGATC AGTCTGCAGC ACAGCGCAGA 
AGTCAGATGG ACCGATTGGA TCGAGAAGAA GCTTTCTATC AATTTGTAAA TAACCTGAGT 
GAAGAAGATT ATAGGCTTAT GAGAGATAAC AATTTGCTAG GCACCCCAGG TGAAAGTACT 
GAGGAAGAGT TGCTGAGACG ACTACAGCAA ATTAAAGAAG GCCCACCACC GCAAAACTCA 
GATGAAAATA GAGGAGGAGA CTCTTCAGAT GATGTGTCTA ATGGTGACTC TATAATAGAC 
TGGCTTAACT CTGTCAGACA AACTGGAAAT ACAACAAGAA GTGGGCAAAG AGGAAACCAA 
TCTTGGAGAG CAGTGAGTCG GACTAATCCA AACAGTGGTG ATTTCAGATT CAGTTTAGAG 
ATAAATGTTA ACCGTAATAA TGGGAGCCAA AATTCAGAGA ATGAAAATGA GCCATCTGCA 
AGACGTTCTA GTGGAGAAAA TGTGGAAAAC AACAGCCAAA GGCAAGTGGA AAACCCACGA 
TCTGAATCAA CATCTGCAAG GCCATCTAGA TCAGAACGAA ATTCAACTGA AGCGTTAACA 
GAGGTCCCAC CTACCAGAGG TCAGAGGAGG GCAAGAAGCA GGAGCCCAGA CCATCGGAGA 
ACCAGAGCAA GAGCTGAAAG AAGTAGGTCA CCTCTGCATC CAATGAGTGA AATTCCACGA 
AGATCTCATC ATAGTATCTC ATCTCAGACT TTTGAACATC CTTTGGTAAA TGAGACGGAG 
GGAAGTTCTA GAACCCGGCA CCATGTGACA TTGAGGCAGC AAATATCTGG GCCTGAGTTG 
CTAAGTAGAG GTCTTTTTGC AGCTTCTGGA ACAAGAAATG CTTCTCAAGG AGCAGGTTCT 
TCAGACACAG CTGCCAGTGG TGAATCTACA GGATCAGGAC AGAGACCTCC AACCATAGTC 
CTTGATCTTC AAGTAAGAAG AGTTCGTCCT GGAGAATATC GGCAGAGAGA TAGCATAGCC 
AGCAGAACTC GGTCTAGGTC TCAGACACCA AACAACACTG TCACCTATGA AAGTGAACGA 
GGAGGTTTTA GGCGTACATT TTCACGTTCT GAGCGGGCAG GTGTGAGAAC CTATGTCAGT 
ACCATCAGAA TTCCCATTCG TAGAATCTTA AATACTGGTT TAAGTGAGAC TACATCTGTT 
GCAATTCAGA CCATGTTAAG GCAGATAATG ACAGGTTTTG GTGAGTTAAG CTATTTTATG 
TACAGTGATA GCGACTCAGA GCCTACTGGC TCAGTCTCAA ATCGAAATAT GGAAAGGGCA 
GAGTCACGGA GTGGAAGAGG AGGTTCTGGT GGTGGTAGTA GTTCTGGTTC CAGTTCGAGT 
TCCAGTTCCA GTTCGAGTTC CAGTTCCAGT TCAAGTTCCA GTTCCAGTCC TAGTTCCAGT 
TCCGGTGGTG AAAGTTCAGA AACTAGCTCA GATTTATTTG AAGGCAGTAA TGAAGGAAGC 
TCATCATCAG GCTCATCAGG TGCCAGGCGA GAGGGTCGAC ATAGGGCCCC AGTCACATTT 
GATGAAAGTG GCTCTTTGCC CTTCCTTAGC CTGGCTCAGT TTTTCCTCTT AAATGAGGAT 
GATGATGACC AACCTAGAGG ACTCACCAAA GAACAGATTG ACAACTTGGC AATGAGAAGT 
TTTGGTGAAA ATGATGCATT AAAAACCTGT AGTGTTTGCA TTACAGAATA TACAGAAGGC 
AACAAACTTC GTAAACTACC TTGTTCCCAT GAGTACCATG TCCACTGCAT CGATCGCTGG 
TTATCTGAGA ATTCTACCTG TCCTATTTGT CGCAGAGCAG TCTTAGCTTC TGGTAACAGA 
GAAAGTGTTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNF12 promotes p53-dependent cell growth suppression and apoptosis by targeting MDM2 for destruction.
Cancer letters375(1)133-141(2016 May)
Gao K,Wang C,Jin X,Xiao J,Zhang E,Yang X,Wang D,Huang H,Yu L,Zhang P

Syndromic X-linked intellectual disability segregating with a missense variant in RLIM.
European journal of human genetics : EJHG23(12)1652-6(2015 Dec)
T?nne E,Holdhus R,Stansberg C,Stray-Pedersen A,Petersen K,Brunner HG,Gilissen C,Hoischen A,Prescott T,Steen VM,Fiskerstrand T

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
PLoS genetics11(8)e1005428(2015 Aug)
Hasegawa Y,Taylor D,Ovchinnikov DA,Wolvetang EJ,de Torrent? L,Mar JC

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RLIM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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