APIP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APIP
Entrez Gene ID	51074
Full Name	APAF1 interacting protein
Synonyms	APIP2,CGI-29,CGI29,MMRP19,hAPIP
General protein information	Preferred Names APAF1 interacting protein Names methylthioribulose-1-phosphate dehydratase MTRu-1-P dehydratase probable methylthioribulose-1-phosphate dehydratase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p13
Summary	APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008].
Disorder MIM:	612491

mRNA and Protein(s)

mRNA	Protein	Name
NM_015957.3	NP_057041.2	methylthioribulose-1-phosphate dehydratase
XM_011520154.3	XP_011518456.1	methylthioribulose-1-phosphate dehydratase isoform X1
XM_017017875.1	XP_016873364.1	methylthioribulose-1-phosphate dehydratase isoform X2
NM_015957.4	NP_057041.2	methylthioribulose-1-phosphate dehydratase
XM_017017875.2	XP_016873364.1	methylthioribulose-1-phosphate dehydratase isoform X2
XM_011520154.2	XP_011518456.1	methylthioribulose-1-phosphate dehydratase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APIP	XP_508364.2
Drosophila melanogaster (fruit fly)	CG11134	NP_572916.1
Caenorhabditis elegans (roundworm)	ZC373.5	NP_509690.2
Xenopus tropicalis (tropical clawed frog)	apip	NP_001015712.1
Homo sapiens (human)	APIP	NP_057041.2
Gallus gallus (chicken)	APIP	NP_001026718.1
Schizosaccharomyces pombe (fission yeast)	SPAC20H4.05c	NP_593625.2
Oryza sativa (rice)	Os11g0484000	NP_001067908.1
Mus musculus (house mouse)	Apip	NP_062709.3
Anopheles gambiae (African malaria mosquito)	MTNB_ANOGA	XP_310624.5
Saccharomyces cerevisiae (baker's yeast)	MDE1	NP_012558.3
Eremothecium gossypii	AGOS_ACL126W	NP_983278.1
Magnaporthe oryzae (rice blast fungus)	MGG_01608	XP_003714592.1
Neurospora crassa	NCU09264	XP_964699.1
Canis lupus familiaris (dog)	APIP	XP_005631172.1
Bos taurus (cattle)	APIP	NP_001068820.1
Danio rerio (zebrafish)	apip	NP_001004679.1
Arabidopsis thaliana (thale cress)	AT5G53850	NP_974931.1

Gene Ontology
Bibliography

Related articles in PubMed

APIP, an ERBB3-binding partner, stimulates erbB2-3 heterodimer formation to promote tumorigenesis.
Hong SH, Lee WJ, Kim YD, Kim H, Jeon YJ, Lim B, Cho DH, Heo WD, Yang DH, Kim CY, Yang HK, Yang JK, Jung YK
Oncotarget7(16)21601-17(2016 Apr)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
Kang W, Hong SH, Lee HM, Kim NY, Lim YC, Le le TM, Lim B, Kim HC, Kim TY, Ashida H, Yokota A, Hah SS, Chun KH, Jung YK, Yang JK
Proceedings of the National Academy of Sciences of the United States of America111(1)E54-61(2014 Jan)

Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein.
Kang W, Yang JK
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 12)1518-20(2012 Dec)

Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway.
Mary C, Duek P, Salleron L, Tienz P, Bumann D, Bairoch A, Lane L
PloS one7(12)e52877(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that APIP stimulates cell proliferation via ERBB3, acting in conjunction with ERBB2, in gastric cancer cells.
Title: APIP, an ERBB3-binding partner, stimulates erbB2-3 heterodimer formation to promote tumorigenesis.

APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis
Title: Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.

human APIP was overproduced in Escherichia coli, purified and crystallized. An X-ray diffraction data set was collected to 2.40 A resolution
Title: Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein.

A polymorphism associated with reduced expression of a putative methionine salvage pathway dehydratase, apoptotic protease activating factor 1 (APAF1)-interacting protein (APIP), was associated with increased caspase-1-mediated cell death in Salmonella.
Title: Functional genetic screen of human diversity reveals that a methionine salvage enzyme regulates inflammatory cell death.

these results confirm the involvement of APIP in the methionine salvage pathway, which plays a key role in many biological functions like cancer, apoptosis, microbial proliferation and inflammation.
Title: Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway.

The following APIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12242	NM_015957.4 Latest version!	Homo sapiens APAF1 interacting protein (APIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu67632	XM_017017875.2 Latest version!	Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu67631	XM_011520154.3 Latest version!	Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu12242	NM_015957.3	Homo sapiens APAF1 interacting protein (APIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu67632	XM_017017875.1	Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu67631	XM_011520154.2	Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APIP ( NM_015957.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057041.2 Homo sapiens APAF1 interacting protein (APIP), mRNA.

Price & Availability↑

CloneID	OHu12242
Clone ID Related Accession (Same CDS sequence)	NM_015957.3 , NM_015957.4
Accession Version	NM_015957.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107928.3, BP200131.1, BC009077.1 and AF088034.1. On Jan 9, 2014 this sequence version replaced NM_015957.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.500804.1, SRR1803616.212640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGCT GTGATGCTCG GGAGGGAGAC TGTTGTTCCC GGAGATGCGG CGCGCAGGAC 
AAGGAGCATC CAAGATACCT GATCCCAGAA CTTTGCAAAC AGTTTTACCA TTTAGGCTGG 
GTCACTGGGA CTGGAGGAGG AATTAGCTTG AAGCATGGCG ATGAAATCTA CATTGCTCCT 
TCAGGAGTGC AAAAGGAACG AATTCAGCCT GAAGACATGT TTGTTTGTGA TATAAATGAA 
AAGGACATAA GTGGACCTTC GCCATCGAAG AAGCTAAAAA AAAGCCAGTG TACTCCTCTT 
TTCATGAATG CTTACACAAT GAGAGGAGCA GGTGCAGTGA TTCATACCCA CTCTAAAGCT 
GCTGTGATGG CCACACTTCT CTTTCCAGGA CGGGAGTTTA AAATTACACA TCAAGAGATG 
ATAAAAGGAA TAAAGAAATG TACTTCCGGA GGGTATTATA GATATGATGA TATGTTAGTG 
GTACCCATTA TTGAGAATAC ACCTGAGGAG AAAGACCTCA AAGATAGAAT GGCTCATGCA 
ATGAATGAAT ACCCAGACTC CTGTGCAGTA CTGGTCAGAC GTCATGGAGT ATATGTGTGG 
GGGGAAACAT GGGAGAAGGC CAAAACCATG TGTGAGTGTT ATGACTATTT ATTTGATATT 
GCCGTATCAA TGAAGAAAGT AGGACTTGAT CCTTCACAGC TCCCAGTTGG AGAAAATGGA 
ATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057041.2
CDS	128..856
Translation	MSGCDAREGDCCSRRCGAQDKEHPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	NM_015957.3
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015957.3

ORF Insert Sequence:

ATGTCTGGCT GTGATGCTCG GGAGGGAGAC TGTTGTTCCC GGAGATGCGG CGCGCAGGAC 
AAGGAGCATC CAAGATACCT GATCCCAGAA CTTTGCAAAC AGTTTTACCA TTTAGGCTGG 
GTCACTGGGA CTGGAGGAGG AATTAGCTTG AAGCATGGCG ATGAAATCTA CATTGCTCCT 
TCAGGAGTGC AAAAGGAACG AATTCAGCCT GAAGACATGT TTGTTTGTGA TATAAATGAA 
AAGGACATAA GTGGACCTTC GCCATCGAAG AAGCTAAAAA AAAGCCAGTG TACTCCTCTT 
TTCATGAATG CTTACACAAT GAGAGGAGCA GGTGCAGTGA TTCATACCCA CTCTAAAGCT 
GCTGTGATGG CCACACTTCT CTTTCCAGGA CGGGAGTTTA AAATTACACA TCAAGAGATG 
ATAAAAGGAA TAAAGAAATG TACTTCCGGA GGGTATTATA GATATGATGA TATGTTAGTG 
GTACCCATTA TTGAGAATAC ACCTGAGGAG AAAGACCTCA AAGATAGAAT GGCTCATGCA 
ATGAATGAAT ACCCAGACTC CTGTGCAGTA CTGGTCAGAC GTCATGGAGT ATATGTGTGG 
GGGGAAACAT GGGAGAAGGC CAAAACCATG TGTGAGTGTT ATGACTATTT ATTTGATATT 
GCCGTATCAA TGAAGAAAGT AGGACTTGAT CCTTCACAGC TCCCAGTTGG AGAAAATGGA 
ATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APIP ( NM_015957.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057041.2 Homo sapiens APAF1 interacting protein (APIP), mRNA.

Price & Availability↑

CloneID	OHu12242
Clone ID Related Accession (Same CDS sequence)	NM_015957.3 , NM_015957.4
Accession Version	NM_015957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107928.3, BP200131.1, BC009077.1 and AF088034.1. On Nov 22, 2018 this sequence version replaced NM_015957.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.129302.1, SRR5189667.244568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395787.4/ ENSP00000379133.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGCT GTGATGCTCG GGAGGGAGAC TGTTGTTCCC GGAGATGCGG CGCGCAGGAC 
AAGGAGCATC CAAGATACCT GATCCCAGAA CTTTGCAAAC AGTTTTACCA TTTAGGCTGG 
GTCACTGGGA CTGGAGGAGG AATTAGCTTG AAGCATGGCG ATGAAATCTA CATTGCTCCT 
TCAGGAGTGC AAAAGGAACG AATTCAGCCT GAAGACATGT TTGTTTGTGA TATAAATGAA 
AAGGACATAA GTGGACCTTC GCCATCGAAG AAGCTAAAAA AAAGCCAGTG TACTCCTCTT 
TTCATGAATG CTTACACAAT GAGAGGAGCA GGTGCAGTGA TTCATACCCA CTCTAAAGCT 
GCTGTGATGG CCACACTTCT CTTTCCAGGA CGGGAGTTTA AAATTACACA TCAAGAGATG 
ATAAAAGGAA TAAAGAAATG TACTTCCGGA GGGTATTATA GATATGATGA TATGTTAGTG 
GTACCCATTA TTGAGAATAC ACCTGAGGAG AAAGACCTCA AAGATAGAAT GGCTCATGCA 
ATGAATGAAT ACCCAGACTC CTGTGCAGTA CTGGTCAGAC GTCATGGAGT ATATGTGTGG 
GGGGAAACAT GGGAGAAGGC CAAAACCATG TGTGAGTGTT ATGACTATTT ATTTGATATT 
GCCGTATCAA TGAAGAAAGT AGGACTTGAT CCTTCACAGC TCCCAGTTGG AGAAAATGGA 
ATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057041.2
CDS	96..824
Translation	MSGCDAREGDCCSRRCGAQDKEHPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	NM_015957.4
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015957.4

ORF Insert Sequence:

ATGTCTGGCT GTGATGCTCG GGAGGGAGAC TGTTGTTCCC GGAGATGCGG CGCGCAGGAC 
AAGGAGCATC CAAGATACCT GATCCCAGAA CTTTGCAAAC AGTTTTACCA TTTAGGCTGG 
GTCACTGGGA CTGGAGGAGG AATTAGCTTG AAGCATGGCG ATGAAATCTA CATTGCTCCT 
TCAGGAGTGC AAAAGGAACG AATTCAGCCT GAAGACATGT TTGTTTGTGA TATAAATGAA 
AAGGACATAA GTGGACCTTC GCCATCGAAG AAGCTAAAAA AAAGCCAGTG TACTCCTCTT 
TTCATGAATG CTTACACAAT GAGAGGAGCA GGTGCAGTGA TTCATACCCA CTCTAAAGCT 
GCTGTGATGG CCACACTTCT CTTTCCAGGA CGGGAGTTTA AAATTACACA TCAAGAGATG 
ATAAAAGGAA TAAAGAAATG TACTTCCGGA GGGTATTATA GATATGATGA TATGTTAGTG 
GTACCCATTA TTGAGAATAC ACCTGAGGAG AAAGACCTCA AAGATAGAAT GGCTCATGCA 
ATGAATGAAT ACCCAGACTC CTGTGCAGTA CTGGTCAGAC GTCATGGAGT ATATGTGTGG 
GGGGAAACAT GGGAGAAGGC CAAAACCATG TGTGAGTGTT ATGACTATTT ATTTGATATT 
GCCGTATCAA TGAAGAAAGT AGGACTTGAT CCTTCACAGC TCCCAGTTGG AGAAAATGGA 
ATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APIP ( XM_017017875.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873364.1 Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67632
Clone ID Related Accession (Same CDS sequence)	XM_017017875.1 , XM_017017875.2
Accession Version	XM_017017875.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTT GTGATATAAA TGAAAAGGAC ATAAGTGGAC CTTCGCCATC GAAGAAGCTA 
AAAAAAAGCC AGTGTACTCC TCTTTTCATG AATGCTTACA CAATGAGAGG AGCAGGTGCA 
GTGATTCATA CCCACTCTAA AGCTGCTGTG ATGGCCACAC TTCTCTTTCC AGGACGGGAG 
TTTAAAATTA CACATCAAGA GATGATAAAA GGAATAAAGA AATGTACTTC CGGAGGGTAT 
TATAGATATG ATGATATGTT AGTGGTACCC ATTATTGAGA ATACACCTGA GGAGAAAGAC 
CTCAAAGATA GAATGGCTCA TGCAATGAAT GAATACCCAG ACTCCTGTGC AGTACTGGTC 
AGACGTCATG GAGTATATGT GTGGGGGGAA ACATGGGAGA AGGCCAAAAC CATGTGTGAG 
TGTTATGACT ATTTATTTGA TATTGCCGTA TCAATGAAGA AAGTAGGACT TGATCCTTCA 
CAGCTCCCAG TTGGAGAAAA TGGAATTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873364.1
CDS	391..903
Translation	MFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	XM_017017875.1
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017875.1

ORF Insert Sequence:

ATGTTTGTTT GTGATATAAA TGAAAAGGAC ATAAGTGGAC CTTCGCCATC GAAGAAGCTA 
AAAAAAAGCC AGTGTACTCC TCTTTTCATG AATGCTTACA CAATGAGAGG AGCAGGTGCA 
GTGATTCATA CCCACTCTAA AGCTGCTGTG ATGGCCACAC TTCTCTTTCC AGGACGGGAG 
TTTAAAATTA CACATCAAGA GATGATAAAA GGAATAAAGA AATGTACTTC CGGAGGGTAT 
TATAGATATG ATGATATGTT AGTGGTACCC ATTATTGAGA ATACACCTGA GGAGAAAGAC 
CTCAAAGATA GAATGGCTCA TGCAATGAAT GAATACCCAG ACTCCTGTGC AGTACTGGTC 
AGACGTCATG GAGTATATGT GTGGGGGGAA ACATGGGAGA AGGCCAAAAC CATGTGTGAG 
TGTTATGACT ATTTATTTGA TATTGCCGTA TCAATGAAGA AAGTAGGACT TGATCCTTCA 
CAGCTCCCAG TTGGAGAAAA TGGAATTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APIP ( XM_017017875.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873364.1 Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67632
Clone ID Related Accession (Same CDS sequence)	XM_017017875.1 , XM_017017875.2
Accession Version	XM_017017875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTT GTGATATAAA TGAAAAGGAC ATAAGTGGAC CTTCGCCATC GAAGAAGCTA 
AAAAAAAGCC AGTGTACTCC TCTTTTCATG AATGCTTACA CAATGAGAGG AGCAGGTGCA 
GTGATTCATA CCCACTCTAA AGCTGCTGTG ATGGCCACAC TTCTCTTTCC AGGACGGGAG 
TTTAAAATTA CACATCAAGA GATGATAAAA GGAATAAAGA AATGTACTTC CGGAGGGTAT 
TATAGATATG ATGATATGTT AGTGGTACCC ATTATTGAGA ATACACCTGA GGAGAAAGAC 
CTCAAAGATA GAATGGCTCA TGCAATGAAT GAATACCCAG ACTCCTGTGC AGTACTGGTC 
AGACGTCATG GAGTATATGT GTGGGGGGAA ACATGGGAGA AGGCCAAAAC CATGTGTGAG 
TGTTATGACT ATTTATTTGA TATTGCCGTA TCAATGAAGA AAGTAGGACT TGATCCTTCA 
CAGCTCCCAG TTGGAGAAAA TGGAATTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873364.1
CDS	422..934
Translation	MFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	XM_017017875.2
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017875.2

ORF Insert Sequence:

ATGTTTGTTT GTGATATAAA TGAAAAGGAC ATAAGTGGAC CTTCGCCATC GAAGAAGCTA 
AAAAAAAGCC AGTGTACTCC TCTTTTCATG AATGCTTACA CAATGAGAGG AGCAGGTGCA 
GTGATTCATA CCCACTCTAA AGCTGCTGTG ATGGCCACAC TTCTCTTTCC AGGACGGGAG 
TTTAAAATTA CACATCAAGA GATGATAAAA GGAATAAAGA AATGTACTTC CGGAGGGTAT 
TATAGATATG ATGATATGTT AGTGGTACCC ATTATTGAGA ATACACCTGA GGAGAAAGAC 
CTCAAAGATA GAATGGCTCA TGCAATGAAT GAATACCCAG ACTCCTGTGC AGTACTGGTC 
AGACGTCATG GAGTATATGT GTGGGGGGAA ACATGGGAGA AGGCCAAAAC CATGTGTGAG 
TGTTATGACT ATTTATTTGA TATTGCCGTA TCAATGAAGA AAGTAGGACT TGATCCTTCA 
CAGCTCCCAG TTGGAGAAAA TGGAATTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APIP ( XM_011520154.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518456.1 Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67631
Clone ID Related Accession (Same CDS sequence)	XM_011520154.3 , XM_011520154.2
Accession Version	XM_011520154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCG CAGTGGCTTA CATGGCTATC AAAATTGAAG TTCAAGGTCA CCAGAGTTTT 
GCAATACCTT CAGGAACATT CTGGCTTTGG CGCTCACTTC TCTCCCAGGA CAAGGAGCAT 
CCAAGATACC TGATCCCAGA ACTTTGCAAA CAGTTTTACC ATTTAGGCTG GGTCACTGGG 
ACTGGAGGAG GAATTAGCTT GAAGCATGGC GATGAAATCT ACATTGCTCC TTCAGGAGTG 
CAAAAGGAAC GAATTCAGCC TGAAGACATG TTTGTTTGTG ATATAAATGA AAAGGACATA 
AGTGGACCTT CGCCATCGAA GAAGCTAAAA AAAAGCCAGT GTACTCCTCT TTTCATGAAT 
GCTTACACAA TGAGAGGAGC AGGTGCAGTG ATTCATACCC ACTCTAAAGC TGCTGTGATG 
GCCACACTTC TCTTTCCAGG ACGGGAGTTT AAAATTACAC ATCAAGAGAT GATAAAAGGA 
ATAAAGAAAT GTACTTCCGG AGGGTATTAT AGATATGATG ATATGTTAGT GGTACCCATT 
ATTGAGAATA CACCTGAGGA GAAAGACCTC AAAGATAGAA TGGCTCATGC AATGAATGAA 
TACCCAGACT CCTGTGCAGT ACTGGTCAGA CGTCATGGAG TATATGTGTG GGGGGAAACA 
TGGGAGAAGG CCAAAACCAT GTGTGAGTGT TATGACTATT TATTTGATAT TGCCGTATCA 
ATGAAGAAAG TAGGACTTGA TCCTTCACAG CTCCCAGTTG GAGAAAATGG AATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518456.1
CDS	172..951
Translation	MRRAVAYMAIKIEVQGHQSFAIPSGTFWLWRSLLSQDKEHPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	XM_011520154.3
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520154.3

ORF Insert Sequence:

ATGCGGCGCG CAGTGGCTTA CATGGCTATC AAAATTGAAG TTCAAGGTCA CCAGAGTTTT 
GCAATACCTT CAGGAACATT CTGGCTTTGG CGCTCACTTC TCTCCCAGGA CAAGGAGCAT 
CCAAGATACC TGATCCCAGA ACTTTGCAAA CAGTTTTACC ATTTAGGCTG GGTCACTGGG 
ACTGGAGGAG GAATTAGCTT GAAGCATGGC GATGAAATCT ACATTGCTCC TTCAGGAGTG 
CAAAAGGAAC GAATTCAGCC TGAAGACATG TTTGTTTGTG ATATAAATGA AAAGGACATA 
AGTGGACCTT CGCCATCGAA GAAGCTAAAA AAAAGCCAGT GTACTCCTCT TTTCATGAAT 
GCTTACACAA TGAGAGGAGC AGGTGCAGTG ATTCATACCC ACTCTAAAGC TGCTGTGATG 
GCCACACTTC TCTTTCCAGG ACGGGAGTTT AAAATTACAC ATCAAGAGAT GATAAAAGGA 
ATAAAGAAAT GTACTTCCGG AGGGTATTAT AGATATGATG ATATGTTAGT GGTACCCATT 
ATTGAGAATA CACCTGAGGA GAAAGACCTC AAAGATAGAA TGGCTCATGC AATGAATGAA 
TACCCAGACT CCTGTGCAGT ACTGGTCAGA CGTCATGGAG TATATGTGTG GGGGGAAACA 
TGGGAGAAGG CCAAAACCAT GTGTGAGTGT TATGACTATT TATTTGATAT TGCCGTATCA 
ATGAAGAAAG TAGGACTTGA TCCTTCACAG CTCCCAGTTG GAGAAAATGG AATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APIP ( XM_011520154.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518456.1 Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67631
Clone ID Related Accession (Same CDS sequence)	XM_011520154.3 , XM_011520154.2
Accession Version	XM_011520154.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methylthioribulose-1-phosphate dehydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCG CAGTGGCTTA CATGGCTATC AAAATTGAAG TTCAAGGTCA CCAGAGTTTT 
GCAATACCTT CAGGAACATT CTGGCTTTGG CGCTCACTTC TCTCCCAGGA CAAGGAGCAT 
CCAAGATACC TGATCCCAGA ACTTTGCAAA CAGTTTTACC ATTTAGGCTG GGTCACTGGG 
ACTGGAGGAG GAATTAGCTT GAAGCATGGC GATGAAATCT ACATTGCTCC TTCAGGAGTG 
CAAAAGGAAC GAATTCAGCC TGAAGACATG TTTGTTTGTG ATATAAATGA AAAGGACATA 
AGTGGACCTT CGCCATCGAA GAAGCTAAAA AAAAGCCAGT GTACTCCTCT TTTCATGAAT 
GCTTACACAA TGAGAGGAGC AGGTGCAGTG ATTCATACCC ACTCTAAAGC TGCTGTGATG 
GCCACACTTC TCTTTCCAGG ACGGGAGTTT AAAATTACAC ATCAAGAGAT GATAAAAGGA 
ATAAAGAAAT GTACTTCCGG AGGGTATTAT AGATATGATG ATATGTTAGT GGTACCCATT 
ATTGAGAATA CACCTGAGGA GAAAGACCTC AAAGATAGAA TGGCTCATGC AATGAATGAA 
TACCCAGACT CCTGTGCAGT ACTGGTCAGA CGTCATGGAG TATATGTGTG GGGGGAAACA 
TGGGAGAAGG CCAAAACCAT GTGTGAGTGT TATGACTATT TATTTGATAT TGCCGTATCA 
ATGAAGAAAG TAGGACTTGA TCCTTCACAG CTCCCAGTTG GAGAAAATGG AATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518456.1
CDS	150..929
Translation	MRRAVAYMAIKIEVQGHQSFAIPSGTFWLWRSLLSQDKEHPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Target ORF information:

RefSeq Version	XM_011520154.2
Organism	Homo sapiens(human)
Definition	Homo sapiens APAF1 interacting protein (APIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520154.2

ORF Insert Sequence:

ATGCGGCGCG CAGTGGCTTA CATGGCTATC AAAATTGAAG TTCAAGGTCA CCAGAGTTTT 
GCAATACCTT CAGGAACATT CTGGCTTTGG CGCTCACTTC TCTCCCAGGA CAAGGAGCAT 
CCAAGATACC TGATCCCAGA ACTTTGCAAA CAGTTTTACC ATTTAGGCTG GGTCACTGGG 
ACTGGAGGAG GAATTAGCTT GAAGCATGGC GATGAAATCT ACATTGCTCC TTCAGGAGTG 
CAAAAGGAAC GAATTCAGCC TGAAGACATG TTTGTTTGTG ATATAAATGA AAAGGACATA 
AGTGGACCTT CGCCATCGAA GAAGCTAAAA AAAAGCCAGT GTACTCCTCT TTTCATGAAT 
GCTTACACAA TGAGAGGAGC AGGTGCAGTG ATTCATACCC ACTCTAAAGC TGCTGTGATG 
GCCACACTTC TCTTTCCAGG ACGGGAGTTT AAAATTACAC ATCAAGAGAT GATAAAAGGA 
ATAAAGAAAT GTACTTCCGG AGGGTATTAT AGATATGATG ATATGTTAGT GGTACCCATT 
ATTGAGAATA CACCTGAGGA GAAAGACCTC AAAGATAGAA TGGCTCATGC AATGAATGAA 
TACCCAGACT CCTGTGCAGT ACTGGTCAGA CGTCATGGAG TATATGTGTG GGGGGAAACA 
TGGGAGAAGG CCAAAACCAT GTGTGAGTGT TATGACTATT TATTTGATAT TGCCGTATCA 
ATGAAGAAAG TAGGACTTGA TCCTTCACAG CTCCCAGTTG GAGAAAATGG AATTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APIP, an ERBB3-binding partner, stimulates erbB2-3 heterodimer formation to promote tumorigenesis.
Oncotarget7(16)21601-17(2016 Apr)
Hong SH,Lee WJ,Kim YD,Kim H,Jeon YJ,Lim B,Cho DH,Heo WD,Yang DH,Kim CY,Yang HK,Yang JK,Jung YK

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
Proceedings of the National Academy of Sciences of the United States of America111(1)E54-61(2014 Jan)
Kang W,Hong SH,Lee HM,Kim NY,Lim YC,Le le TM,Lim B,Kim HC,Kim TY,Ashida H,Yokota A,Hah SS,Chun KH,Jung YK,Yang JK

Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein.
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 12)1518-20(2012 Dec)
Kang W,Yang JK

Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway.
PloS one7(12)e52877(2012)
Mary C,Duek P,Salleron L,Tienz P,Bumann D,Bairoch A,Lane L

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APIP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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