MRPL4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL4
Entrez Gene ID	51073
Full Name	mitochondrial ribosomal protein L4
Synonyms	CGI-28,L4mt
General protein information	Preferred Names mitochondrial ribosomal protein L4 Names 39S ribosomal protein L4, mitochondrial MRP-L4 mitochondrial large ribosomal subunit protein uL4m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	611823

mRNA and Protein(s)

mRNA	Protein	Name
NM_146388.2	NP_666500.1	39S ribosomal protein L4, mitochondrial isoform b
NM_015956.3	NP_057040.2	39S ribosomal protein L4, mitochondrial isoform a
NM_015956.2	NP_057040.2	39S ribosomal protein L4, mitochondrial isoform a
NM_146387.1	NP_666499.1	39S ribosomal protein L4, mitochondrial isoform a
XM_011528045.2	XP_011526347.1	39S ribosomal protein L4, mitochondrial isoform X1
NM_146388.1	NP_666500.1	39S ribosomal protein L4, mitochondrial isoform b
XM_011528045.3	XP_011526347.1	39S ribosomal protein L4, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G20060	NP_565463.1
Pan troglodytes (chimpanzee)	MRPL4	XP_001164062.2
Canis lupus familiaris (dog)	MRPL4	XP_854013.1
Rattus norvegicus (Norway rat)	Mrpl4	NP_001102224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007937	XP_317543.4
Homo sapiens (human)	MRPL4	NP_666499.1
Mus musculus (house mouse)	Mrpl4	NP_075656.2
Drosophila melanogaster (fruit fly)	mRpL4	NP_524939.1
Caenorhabditis elegans (roundworm)	mrpl-4	NP_505181.1
Xenopus tropicalis (tropical clawed frog)	mrpl4	NP_001017222.1
Bos taurus (cattle)	MRPL4	NP_001032527.1
Danio rerio (zebrafish)	zgmrpl4	NP_001018572.1
Oryza sativa (rice)	Os11g0585700	NP_001068165.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

The association between polymorphisms in the MRPL4 and TNF-? genes and susceptibility to allergic rhinitis.
Wei X, Zhang Y, Fu Z, Zhang L
PloS one8(3)e57981(2013)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

there is a strong association between allergic rhinitis risk and polymorphisms of MRPL4 and TNF-alpha genes in the Han Chinese population
Title: The association between polymorphisms in the MRPL4 and TNF-? genes and susceptibility to allergic rhinitis.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity.

The following MRPL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08967	NM_146387.1 Latest version!	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu08967	NM_015956.3 Latest version!	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu31522	NM_146388.2 Latest version!	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu67630	XM_011528045.3 Latest version!	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu08967	NM_015956.2	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu31522	NM_146388.1	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu67630	XM_011528045.2	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL4 ( NM_015956.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057040.2 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08967
Clone ID Related Accession (Same CDS sequence)	NM_015956.3 , NM_015956.2 , NM_146387.1
Accession Version	NM_015956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG325905.1, BC009858.2 and AC011511.12. On May 17, 2019 this sequence version replaced NM_015956.2. Transcript Variant: This variant (1) is the predominant transcript and it encodes the same isoform (a) as variant 2. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.633180.1, SRR1163655.419345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000253099.11/ ENSP00000253099.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057040.2
CDS	41..976
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHATQGPAATPYHC

Target ORF information:

RefSeq Version	NM_015956.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015956.3

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( NM_015956.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057040.2 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08967
Clone ID Related Accession (Same CDS sequence)	NM_015956.3 , NM_015956.2 , NM_146387.1
Accession Version	NM_015956.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG325905.1 and BC009858.2. On Aug 29, 2002 this sequence version replaced NM_015956.1. Transcript Variant: This variant (1) is the predominant transcript and it encodes the same isoform (a) as variant 2. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.191971.1, SRR1163658.558558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057040.2
CDS	291..1226
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHATQGPAATPYHC

Target ORF information:

RefSeq Version	NM_015956.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015956.2

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( NM_146387.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_666499.1 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08967
Clone ID Related Accession (Same CDS sequence)	NM_015956.3 , NM_015956.2 , NM_146387.1
Accession Version	NM_146387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG758801.1 and BC009858.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). ##Evidence-Data-START## CDS exon combination :: SRR1163655.197187.1, SRR1163657.426559.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666499.1
CDS	155..1090
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHATQGPAATPYHC

Target ORF information:

RefSeq Version	NM_146387.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146387.1

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG CCTAAATGTG CACAGCATGC TCAAGCACCA GACGCTGGTC 
CTGACGCTGC CCACCGTCGC CTTCCTGGAG GACAAGCTGC TCTGGCAGGA CTCACGTTAC 
AGACCCCTCT ACCCCTTCAG CCTGCCCTAC AGCGACTTCC CCCGACCCCT ACCCCACGCT 
ACCCAGGGCC CAGCGGCCAC CCCGTACCAC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( NM_146388.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_666500.1 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu31522
Clone ID Related Accession (Same CDS sequence)	NM_146388.2 , NM_146388.1
Accession Version	NM_146388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011511.12. On May 31, 2019 this sequence version replaced NM_146388.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes an isoform (b) which has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC000756.2, SRR1163657.563052.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG TGAGCAAAGA GCCCAGGCCC CTAGAGTGCG CATGTGCAGG 
CTCCGCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666500.1
CDS	41..832
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC

Target ORF information:

RefSeq Version	NM_146388.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146388.2

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG TGAGCAAAGA GCCCAGGCCC CTAGAGTGCG CATGTGCAGG 
CTCCGCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( NM_146388.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_666500.1 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.

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CloneID	OHu31522
Clone ID Related Accession (Same CDS sequence)	NM_146388.2 , NM_146388.1
Accession Version	NM_146388.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011511.12. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes an isoform (b) which has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC000756.2, SRR1163658.234872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG TGAGCAAAGA GCCCAGGCCC CTAGAGTGCG CATGTGCAGG 
CTCCGCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666500.1
CDS	291..1082
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC

Target ORF information:

RefSeq Version	NM_146388.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146388.1

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGG TGAGCAAAGA GCCCAGGCCC CTAGAGTGCG CATGTGCAGG 
CTCCGCTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( XM_011528045.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526347.1 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.

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CloneID	OHu67630
Clone ID Related Accession (Same CDS sequence)	XM_011528045.2 , XM_011528045.3
Accession Version	XM_011528045.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGC CACAAGATGG CGACATGAGC CTAAGTCAAG TCCTGTCCCT 
CAACCCACGC CCCTCGCTGG CTTCCCCACC TCTCTCAGGA TGAAAGCCCA AGTCATCAGG 
GTGGCACGTC AGGCCCTGCA CGATGTGCCC CGTCACCTCC CTGCCCTCCC GTCATTTGCC 
CTGGGTCTCC CCCACCAGAA TGGGAGCCAG GAGTCCCAGC CAGGCACAGG ACCGAGCCTG 
GCTCTCGGTC AGCAGGTGAT GAGCTGGGAG CAGCTCCTTG GCCAGAGGCT TACAGGCAAC 
AAGCGGCCAG GCAGGGTCTG GCCCCGGGCT GCTGGGCTCA CAAAGTCACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526347.1
CDS	286..1359
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVATRWRHEPKSSPVPQPTPLAGFPTSLRMKAQVIRVARQALHDVPRHLPALPSFALGLPHQNGSQESQPGTGPSLALGQQVMSWEQLLGQRLTGNKRPGRVWPRAAGLTKSH

Target ORF information:

RefSeq Version	XM_011528045.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528045.2

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGC CACAAGATGG CGACATGAGC CTAAGTCAAG TCCTGTCCCT 
CAACCCACGC CCCTCGCTGG CTTCCCCACC TCTCTCAGGA TGAAAGCCCA AGTCATCAGG 
GTGGCACGTC AGGCCCTGCA CGATGTGCCC CGTCACCTCC CTGCCCTCCC GTCATTTGCC 
CTGGGTCTCC CCCACCAGAA TGGGAGCCAG GAGTCCCAGC CAGGCACAGG ACCGAGCCTG 
GCTCTCGGTC AGCAGGTGAT GAGCTGGGAG CAGCTCCTTG GCCAGAGGCT TACAGGCAAC 
AAGCGGCCAG GCAGGGTCTG GCCCCGGGCT GCTGGGCTCA CAAAGTCACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL4 ( XM_011528045.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526347.1 Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67630
Clone ID Related Accession (Same CDS sequence)	XM_011528045.2 , XM_011528045.3
Accession Version	XM_011528045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	39S ribosomal protein L4, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGC CACAAGATGG CGACATGAGC CTAAGTCAAG TCCTGTCCCT 
CAACCCACGC CCCTCGCTGG CTTCCCCACC TCTCTCAGGA TGAAAGCCCA AGTCATCAGG 
GTGGCACGTC AGGCCCTGCA CGATGTGCCC CGTCACCTCC CTGCCCTCCC GTCATTTGCC 
CTGGGTCTCC CCCACCAGAA TGGGAGCCAG GAGTCCCAGC CAGGCACAGG ACCGAGCCTG 
GCTCTCGGTC AGCAGGTGAT GAGCTGGGAG CAGCTCCTTG GCCAGAGGCT TACAGGCAAC 
AAGCGGCCAG GCAGGGTCTG GCCCCGGGCT GCTGGGCTCA CAAAGTCACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526347.1
CDS	293..1366
Translation	MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVATRWRHEPKSSPVPQPTPLAGFPTSLRMKAQVIRVARQALHDVPRHLPALPSFALGLPHQNGSQESQPGTGPSLALGQQVMSWEQLLGQRLTGNKRPGRVWPRAAGLTKSH

Target ORF information:

RefSeq Version	XM_011528045.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L4 (MRPL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528045.3

ORF Insert Sequence:

ATGCTGCAGT TCGTCCGGGC CGGGGCGCGG GCCTGGCTTC GGCCTACCGG CAGCCAGGGC 
CTGAGTTCCC TGGCGGAAGA GGCAGCGCGT GCGACCGAGA ACCCGGAGCA GGTGGCGAGC 
GAGGGTCTCC CGGAGCCCGT GCTGCGCAAA GTCGAGCTCC CGGTACCCAC TCATCGACGC 
CCAGTGCAGG CCTGGGTCGA GTCCTTGCGG GGCTTCGAGC AGGAGCGCGT GGGCCTGGCC 
GACCTGCACC CCGATGTTTT CGCCACCGCG CCCAGGCTGG ACATACTGCA CCAGGTTGCT 
ATGTGGCAGA AGAACTTCAA GAGAATTAGC TATGCCAAGA CCAAGACGAG AGCCGAGGTG 
CGGGGCGGTG GCCGGAAGCC TTGGCCGCAG AAAGGCACTG GGCGGGCCCG GCATGGCAGC 
ATCCGCTCTC CGCTCTGGCG AGGAGGAGGT GTTGCCCATG GCCCCCGGGG CCCCACAAGT 
TACTACTACA TGCTGCCCAT GAAGGTGCGG GCGCTGGGTC TCAAAGTGGC ACTGACCGTC 
AAGCTGGCCC AGGACGACCT GCACATCATG GACTCCCTAG AGCTGCCCAC CGGAGACCCA 
CAGTACCTGA CAGAGCTGGC GCACTACCGC CGCTGGGGGG ACTCCGTACT CCTCGTGGAC 
TTAACACACG AGGAGATGCC ACAGAGCATC GTGGAGGCCA CCTCTAGGCT TAAGACCTTC 
AACTTGATCC CGGCTGTTGC CACAAGATGG CGACATGAGC CTAAGTCAAG TCCTGTCCCT 
CAACCCACGC CCCTCGCTGG CTTCCCCACC TCTCTCAGGA TGAAAGCCCA AGTCATCAGG 
GTGGCACGTC AGGCCCTGCA CGATGTGCCC CGTCACCTCC CTGCCCTCCC GTCATTTGCC 
CTGGGTCTCC CCCACCAGAA TGGGAGCCAG GAGTCCCAGC CAGGCACAGG ACCGAGCCTG 
GCTCTCGGTC AGCAGGTGAT GAGCTGGGAG CAGCTCCTTG GCCAGAGGCT TACAGGCAAC 
AAGCGGCCAG GCAGGGTCTG GCCCCGGGCT GCTGGGCTCA CAAAGTCACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

The association between polymorphisms in the MRPL4 and TNF-? genes and susceptibility to allergic rhinitis.
PloS one8(3)e57981(2013)
Wei X,Zhang Y,Fu Z,Zhang L

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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MRPL4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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