PCBP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCBP1
Entrez Gene ID	5093
Full Name	poly(rC) binding protein 1
Synonyms	HEL-S-85,HNRPE1,HNRPX,hnRNP-E1,hnRNP-X
General protein information	Preferred Names poly(rC) binding protein 1 Names poly(rC)-binding protein 1 alpha-CP1 epididymis secretory protein Li 85 heterogeneous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein X nucleic acid-binding protein SUB2.3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.3
Summary	This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008].
Disorder MIM:	601209

mRNA and Protein(s)

mRNA	Protein	Name
NM_006196.4	NP_006187.2	poly(rC)-binding protein 1
NM_006196.3	NP_006187.2	poly(rC)-binding protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCBP1	NP_006187.2
Canis lupus familiaris (dog)	PCBP1	XP_538537.1
Bos taurus (cattle)	PCBP1	NP_001015565.1
Rattus norvegicus (Norway rat)	Pcbp1	XP_006236906.1
Pan troglodytes (chimpanzee)	PCBP1	XP_515530.2
Mus musculus (house mouse)	Pcbp1	NP_035995.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of both poly r(C) binding protein 1 (PCBP1) and miRNA-3978 is suppressed in peritoneal gastric cancer metastasis.
Ji FJ, Wu YY, An Z, Liu XS, Jiang JN, Chen FF, Fang XD
Scientific reports7(1)15488(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest a 216-nucleotide proximal cis-element in LIF mRNA exhibits mRNA destabilizing potential; on exposure to carcinogen PMA (phorbol-12-myristate-13-acetate), this cis-element exhibits mRNA stabilizing activity. PMA induces nucleo-cytoplasmic translocation of both nucleolin and PCBP1, 2 trans-acting factors that bind to and stabilize LIF mRNA. [LIF = leukemia inhibitory factor; PCBP1 = poly(rC) binding protein 1]
Title: Phorbol-12-myristate-13-acetate-mediated stabilization of leukemia inhibitory factor (

The results provide a mechanism for exon 16 3' splice site activation in which a coordinated effort among TIA1, Pcbp1, and RBM39 stabilizes or increases U2 snRNP recruitment, enhances spliceosome A complex formation, and facilitates exon definition through RBM39-mediated splicing regulation.
Title: Protein 4.1R Exon 16 3' Splice Site Activation Requires Coordination among TIA1, Pcbp1, and RBM39 during Terminal Erythropoiesis.

It was concluded that a novel positive feedback loop amplifies hnRNP-E1 during prolonged folate deficiency and thereby maximizes upregulation of folate receptors in order to restore folate homeostasis toward normalcy in placental cells.
Title: Evidence Favoring a Positive Feedback Loop for Physiologic Auto Upregulation of hnRNP-E1 during Prolonged Folate Deficiency in Human Placental Cells.

our findings suggest that PCBP1 is a molecular marker of oxaliplatin resistance in colorectal cancer
Title: Poly(C)-binding protein 1 mediates drug resistance in colorectal cancer.

Study report a novel interaction between mutant HSPB1-P182L and the RNA binding protein PCBP1, leading to a reduction in its translational repression activity. Identifying PCBP1 mRNA targets revealed a marked prevalence for an RNA recognition motif, preferably seen in their 5' and 3'UTRs. Findings further support a role for mutant HSPB1 in neurodegenerative diseases.
Title: Mutant HSPB1 causes loss of translational repression by binding to PCBP1, an RNA binding protein with a possible role in neurodegenerative disease.

The following PCBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18556	NM_006196.4 Latest version!	Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu18556	NM_006196.3	Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCBP1 ( NM_006196.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006187.2 Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.

Price & Availability↑

CloneID	OHu18556
Clone ID Related Accession (Same CDS sequence)	NM_006196.4 , NM_006196.3
Accession Version	NM_006196.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	poly(rC)-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM794597.1, BC039742.1 and Z29505.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_006196.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1163657.369363.1, SRR1163655.462887.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303577.7/ ENSP00000305556.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATGCCG GTGTGACTGA AAGTGGACTA AATGTGACTC TCACCATTCG GCTTCTTATG 
CACGGAAAGG AAGTAGGAAG CATCATTGGG AAGAAAGGGG AGTCGGTTAA GAGGATCCGC 
GAGGAGAGTG GCGCGCGGAT CAACATCTCG GAGGGGAATT GTCCGGAGAG AATCATCACT 
CTGACCGGCC CCACCAATGC CATCTTTAAG GCTTTCGCTA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACCGCGGCCA GCAGGCCCCC GGTCACCCTG 
AGGCTGGTGG TGCCGGCCAC CCAGTGCGGC TCCCTGATTG GGAAAGGCGG GTGTAAGATC 
AAAGAGATCC GCGAGAGTAC GGGGGCGCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCCACCGAGC GGGCCATCAC CATCGCTGGC GTGCCGCAGT CTGTCACCGA GTGTGTCAAG 
CAGATTTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CGCAAGGGAG AGTCATGACC 
ATTCCGTACC AGCCCATGCC GGCCAGCTCC CCAGTCATCT GCGCGGGCGG CCAAGATCGG 
TGCAGCGACG CTGCGGGCTA CCCCCATGCC ACCCATGACC TGGAGGGACC ACCTCTAGAT 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTCG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACCGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATTGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATTAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCAGTGGAA 
GGCTCCTCTG GTAGGCAGGT TACTATCACT GGCTCTGCTG CCAGTATTAG TCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGCATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006187.2
CDS	268..1338
Translation	MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

Target ORF information:

RefSeq Version	NM_006196.4
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006196.4

ORF Insert Sequence:

ATGGATGCCG GTGTGACTGA AAGTGGACTA AATGTGACTC TCACCATTCG GCTTCTTATG 
CACGGAAAGG AAGTAGGAAG CATCATTGGG AAGAAAGGGG AGTCGGTTAA GAGGATCCGC 
GAGGAGAGTG GCGCGCGGAT CAACATCTCG GAGGGGAATT GTCCGGAGAG AATCATCACT 
CTGACCGGCC CCACCAATGC CATCTTTAAG GCTTTCGCTA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACCGCGGCCA GCAGGCCCCC GGTCACCCTG 
AGGCTGGTGG TGCCGGCCAC CCAGTGCGGC TCCCTGATTG GGAAAGGCGG GTGTAAGATC 
AAAGAGATCC GCGAGAGTAC GGGGGCGCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCCACCGAGC GGGCCATCAC CATCGCTGGC GTGCCGCAGT CTGTCACCGA GTGTGTCAAG 
CAGATTTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CGCAAGGGAG AGTCATGACC 
ATTCCGTACC AGCCCATGCC GGCCAGCTCC CCAGTCATCT GCGCGGGCGG CCAAGATCGG 
TGCAGCGACG CTGCGGGCTA CCCCCATGCC ACCCATGACC TGGAGGGACC ACCTCTAGAT 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTCG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACCGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATTGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATTAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCAGTGGAA 
GGCTCCTCTG GTAGGCAGGT TACTATCACT GGCTCTGCTG CCAGTATTAG TCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGCATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCBP1 ( NM_006196.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006187.2 Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.

Price & Availability↑

CloneID	OHu18556
Clone ID Related Accession (Same CDS sequence)	NM_006196.4 , NM_006196.3
Accession Version	NM_006196.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	poly(rC)-binding protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM794597.1, BC039742.1 and Z29505.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2009 this sequence version replaced NM_006196.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGATGCCG GTGTGACTGA AAGTGGACTA AATGTGACTC TCACCATTCG GCTTCTTATG 
CACGGAAAGG AAGTAGGAAG CATCATTGGG AAGAAAGGGG AGTCGGTTAA GAGGATCCGC 
GAGGAGAGTG GCGCGCGGAT CAACATCTCG GAGGGGAATT GTCCGGAGAG AATCATCACT 
CTGACCGGCC CCACCAATGC CATCTTTAAG GCTTTCGCTA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACCGCGGCCA GCAGGCCCCC GGTCACCCTG 
AGGCTGGTGG TGCCGGCCAC CCAGTGCGGC TCCCTGATTG GGAAAGGCGG GTGTAAGATC 
AAAGAGATCC GCGAGAGTAC GGGGGCGCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCCACCGAGC GGGCCATCAC CATCGCTGGC GTGCCGCAGT CTGTCACCGA GTGTGTCAAG 
CAGATTTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CGCAAGGGAG AGTCATGACC 
ATTCCGTACC AGCCCATGCC GGCCAGCTCC CCAGTCATCT GCGCGGGCGG CCAAGATCGG 
TGCAGCGACG CTGCGGGCTA CCCCCATGCC ACCCATGACC TGGAGGGACC ACCTCTAGAT 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTCG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACCGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATTGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATTAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCAGTGGAA 
GGCTCCTCTG GTAGGCAGGT TACTATCACT GGCTCTGCTG CCAGTATTAG TCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGCATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006187.2
CDS	292..1362
Translation	MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

Target ORF information:

RefSeq Version	NM_006196.3
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006196.3

ORF Insert Sequence:

ATGGATGCCG GTGTGACTGA AAGTGGACTA AATGTGACTC TCACCATTCG GCTTCTTATG 
CACGGAAAGG AAGTAGGAAG CATCATTGGG AAGAAAGGGG AGTCGGTTAA GAGGATCCGC 
GAGGAGAGTG GCGCGCGGAT CAACATCTCG GAGGGGAATT GTCCGGAGAG AATCATCACT 
CTGACCGGCC CCACCAATGC CATCTTTAAG GCTTTCGCTA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACCGCGGCCA GCAGGCCCCC GGTCACCCTG 
AGGCTGGTGG TGCCGGCCAC CCAGTGCGGC TCCCTGATTG GGAAAGGCGG GTGTAAGATC 
AAAGAGATCC GCGAGAGTAC GGGGGCGCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCCACCGAGC GGGCCATCAC CATCGCTGGC GTGCCGCAGT CTGTCACCGA GTGTGTCAAG 
CAGATTTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CGCAAGGGAG AGTCATGACC 
ATTCCGTACC AGCCCATGCC GGCCAGCTCC CCAGTCATCT GCGCGGGCGG CCAAGATCGG 
TGCAGCGACG CTGCGGGCTA CCCCCATGCC ACCCATGACC TGGAGGGACC ACCTCTAGAT 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTCG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACCGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATTGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATTAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCAGTGGAA 
GGCTCCTCTG GTAGGCAGGT TACTATCACT GGCTCTGCTG CCAGTATTAG TCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGCATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of both poly r(C) binding protein 1 (PCBP1) and miRNA-3978 is suppressed in peritoneal gastric cancer metastasis.
Scientific reports7(1)15488(2017 Nov)
Ji FJ,Wu YY,An Z,Liu XS,Jiang JN,Chen FF,Fang XD

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PCBP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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