DHH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHH
Entrez Gene ID	50846
Full Name	desert hedgehog
Synonyms	GDXYM,HHG-3,SRXY7
General protein information	Preferred Names desert hedgehog Names desert hedgehog protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010].
Disorder MIM:	605423
Disorder Html:	46XY partial gonadal dysgenesis, with minifascicular neuropathy, 607080 (3); 46XY sex reversal 7, 233420 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021044.3	NP_066382.1	desert hedgehog protein preproprotein
XM_017019380.1	XP_016874869.1	desert hedgehog protein isoform X1
NM_021044.4	NP_066382.1	desert hedgehog protein preproprotein
NM_021044.2	NP_066382.1	desert hedgehog protein preproprotein
XM_017019381.1	XP_016874870.1	desert hedgehog protein isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DHH	XP_002687352.1
Canis lupus familiaris (dog)	DHH	XP_003640009.1
Rattus norvegicus (Norway rat)	Dhh	NP_445819.1
Homo sapiens (human)	DHH	NP_066382.1
Mus musculus (house mouse)	Dhh	NP_031883.1
Xenopus tropicalis (tropical clawed frog)	dhh	NP_001090638.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel variant of DHH in a familial case of 46,XY disorder of sex development: Insights from molecular dynamics simulations.
Paris F, Flatters D, Caburet S, Legois B, Servant N, Lefebvre H, Sultan C, Veitia RA
Clinical endocrinology87(5)539-544(2017 Nov)

46,XY Gonadal Dysgenesis due to a Homozygous Mutation in Desert Hedgehog (DHH) Identified by Exome Sequencing.
Werner R, Merz H, Birnbaum W, Marshall L, Schr?der T, Reiz B, Kavran JM, B?umer T, Capetian P, Hiort O
The Journal of clinical endocrinology and metabolism100(7)E1022-9(2015 Jul)

A genetic variant in 12q13, a possible risk factor for bipolar disorder, is associated with depressive state, accounting for stressful life events.
Shimasaki A, Kondo K, Saito T, Esaki K, Otsuka Y, Mano K, Ikeda M, Iwata N
PloS one9(12)e115135(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in DHH play a role in 46,XY gonadal dysgenesis and are associated with seminoma formation and a neuropathy with minifascicle formation.
Title: 46,XY Gonadal Dysgenesis due to a Homozygous Mutation in Desert Hedgehog (DHH) Identified by Exome Sequencing.

Single nucleotide polymorphism in the DHH gene is associated with bipolar disorder.
Title: A genetic variant in 12q13, a possible risk factor for bipolar disorder, is associated with depressive state, accounting for stressful life events.

Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML.
Title: DHH-RHEBL1 fusion transcript: a novel recurrent feature in the new landscape of pediatric CBFA2T3-GLIS2-positive acute myeloid leukemia.

Mutations in DHH are associated with 46,XY pure gonadal dysgenesis and mixed gonadal dysgenesis.
Title: In vitro and molecular modeling analysis of two mutant desert hedgehog proteins associated with 46,XY gonadal dysgenesis.

This study demonistrated that Desert hedgehog links transcription factor Sox10 to peripheral nerve development.
Title: Desert hedgehog links transcription factor Sox10 to perineurial development.

The following DHH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18499	NM_021044.4 Latest version!	Homo sapiens desert hedgehog signaling molecule (DHH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu83828	XM_017019381.1 Latest version!	Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu83827	XM_017019380.1 Latest version!	Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu18499	NM_021044.3	Homo sapiens desert hedgehog (DHH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu18499	NM_021044.2	Homo sapiens desert hedgehog (DHH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHH ( NM_021044.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066382.1 Homo sapiens desert hedgehog (DHH), mRNA.

Price & Availability↑

CloneID	OHu18499
Clone ID Related Accession (Same CDS sequence)	NM_021044.3 , NM_021044.4 , NM_021044.2
Accession Version	NM_021044.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	desert hedgehog protein preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033507.1, AC011603.37 and AW182088.1. This sequence is a reference standard in the RefSeqGene project. On Mar 26, 2016 this sequence version replaced NM_021044.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033507.1, AK313360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066382.1
CDS	308..1498
Translation	MALLTNLLPLCCLALLALPAQSCGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG

Target ORF information:

RefSeq Version	NM_021044.3
Organism	Homo sapiens(human)
Definition	Homo sapiens desert hedgehog (DHH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021044.3

ORF Insert Sequence:

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHH ( NM_021044.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066382.1 Homo sapiens desert hedgehog signaling molecule (DHH), mRNA.

Price & Availability↑

CloneID	OHu18499
Clone ID Related Accession (Same CDS sequence)	NM_021044.3 , NM_021044.4 , NM_021044.2
Accession Version	NM_021044.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	desert hedgehog protein preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033507.1, AC011603.37 and AW182088.1. This sequence is a reference standard in the RefSeqGene project. On Aug 9, 2018 this sequence version replaced NM_021044.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033507.1, HQ607677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000649637.2/ ENSP00000497483.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066382.1
CDS	290..1480
Translation	MALLTNLLPLCCLALLALPAQSCGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG

Target ORF information:

RefSeq Version	NM_021044.4
Organism	Homo sapiens(human)
Definition	Homo sapiens desert hedgehog signaling molecule (DHH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021044.4

ORF Insert Sequence:

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHH ( NM_021044.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_066382.1 Homo sapiens desert hedgehog (DHH), mRNA.

Price & Availability↑

CloneID	OHu18499
Clone ID Related Accession (Same CDS sequence)	NM_021044.3 , NM_021044.4 , NM_021044.2
Accession Version	NM_021044.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	desert hedgehog protein preproprotein
Comment

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066382.1
Translation	MALLTNLLPLCCLALLALPAQSCGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG

Target ORF information:

RefSeq Version	NM_021044.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens desert hedgehog (DHH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021044.2

ORF Insert Sequence:

ATGGCTCTCC TGACCAATCT ACTGCCCCTG TGCTGCTTGG CACTTCTGGC GCTGCCAGCC 
CAGAGCTGCG GGCCGGGCCG GGGGCCGGTT GGCCGGCGCC GCTATGCGCG CAAGCAGCTC 
GTGCCGCTAC TCTACAAGCA ATTTGTGCCC GGCGTGCCAG AGCGGACCCT GGGCGCCAGT 
GGGCCAGCGG AGGGGAGGGT GGCAAGGGGC TCCGAGCGCT TCCGGGACCT CGTGCCCAAC 
TACAACCCCG ACATCATCTT CAAGGATGAG GAGAACAGTG GAGCCGACCG CCTGATGACC 
GAGCGTTGTA AGGAGCGGGT GAACGCTTTG GCCATTGCCG TGATGAACAT GTGGCCCGGA 
GTGCGCCTAC GAGTGACTGA GGGCTGGGAC GAGGACGGCC ACCACGCTCA GGATTCACTC 
CACTACGAAG GCCGTGCTTT GGACATCACT ACGTCTGACC GCGACCGCAA CAAGTATGGG 
TTGCTGGCGC GCCTCGCAGT GGAAGCCGGC TTCGACTGGG TCTACTACGA GTCCCGCAAC 
CACGTCCACG TGTCGGTCAA AGCTGATAAC TCACTGGCGG TCCGGGCGGG CGGCTGCTTT 
CCGGGAAATG CAACTGTGCG CCTGTGGAGC GGCGAGCGGA AAGGGCTGCG GGAACTGCAC 
CGCGGAGACT GGGTTTTGGC GGCCGATGCG TCAGGCCGGG TGGTGCCCAC GCCGGTGCTG 
CTCTTCCTGG ACCGGGACTT GCAGCGCCGG GCTTCATTTG TGGCTGTGGA GACCGAGTGG 
CCTCCACGCA AACTGTTGCT CACGCCCTGG CACCTGGTGT TTGCCGCTCG AGGGCCGGCG 
CCCGCGCCAG GCGACTTTGC ACCGGTGTTC GCGCGCCGGC TACGCGCTGG GGACTCGGTG 
CTGGCGCCCG GCGGGGATGC GCTTCGGCCA GCGCGCGTGG CCCGTGTGGC GCGGGAGGAA 
GCCGTGGGCG TGTTCGCGCC GCTCACCGCG CACGGGACGC TGCTGGTGAA CGATGTCCTG 
GCCTCTTGCT ACGCGGTTCT GGAGAGTCAC CAGTGGGCGC ACCGCGCTTT TGCCCCCTTG 
AGACTGCTGC ACGCGCTAGG GGCGCTGCTC CCCGGCGGGG CCGTCCAGCC GACTGGCATG 
CATTGGTACT CTCGGCTCCT CTACCGCTTA GCGGAGGAGC TACTGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHH ( XM_017019381.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874870.1 Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X2, mRNA.

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CloneID	OHu83828
Clone ID Related Accession (Same CDS sequence)	XM_017019381.1
Accession Version	XM_017019381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	desert hedgehog protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGT GGCCCGGAGT GCGCCTACGA GTGACTGAGG GCTGGGACGA GGACGGCCAC 
CACGCTCAGG ATTCACTCCA CTACGAAGGC CGTGCTTTGG ACATCACTAC GTCTGACCGC 
GACCGCAACA AGTATGGGTT GCTGGCGCGC CTCGCAGTGG AAGCCGGCTT CGACTGGGTC 
TACTACGAGT CCCGCAACCA CGTCCACGTG TCGGTCAAAG CTGATAACTC ACTGGCGGTC 
CGGGCGGGCG GCTGCTTTCC GGGAAATGCA ACTGTGCGCC TGTGGAGCGG CGAGCGGAAA 
GGGCTGCGGG AACTGCACCG CGGAGACTGG GTTTTGGCGG CCGATGCGTC AGGCCGGGTG 
GTGCCCACGC CGGTGCTGCT CTTCCTGGAC CGGGACTTGC AGCGCCGGGC TTCATTTGTG 
GCTGTGGAGA CCGAGTGGCC TCCACGCAAA CTGTTGCTCA CGCCCTGGCA CCTGGTGTTT 
GCCGCTCGAG GGCCGGCGCC CGCGCCAGGC GACTTTGCAC CGGTGTTCGC GCGCCGGCTA 
CGCGCTGGGG ACTCGGTGCT GGCGCCCGGC GGGGATGCGC TTCGGCCAGC GCGCGTGGCC 
CGTGTGGCGC GGGAGGAAGC CGTGGGCGTG TTCGCGCCGC TCACCGCGCA CGGGACGCTG 
CTGGTGAACG ATGTCCTGGC CTCTTGCTAC GCGGTTCTGG AGAGTCACCA GTGGGCGCAC 
CGCGCTTTTG CCCCCTTGAG ACTGCTGCAC GCGCTAGGGG CGCTGCTCCC CGGCGGGGCC 
GTCCAGCCGA CTGGCATGCA TTGGTACTCT CGGCTCCTCT ACCGCTTAGC GGAGGAGCTA 
CTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874870.1
CDS	298..1146
Translation	MNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG

Target ORF information:

RefSeq Version	XM_017019381.1
Organism	Homo sapiens(human)
Definition	Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019381.1

ORF Insert Sequence:

ATGAACATGT GGCCCGGAGT GCGCCTACGA GTGACTGAGG GCTGGGACGA GGACGGCCAC 
CACGCTCAGG ATTCACTCCA CTACGAAGGC CGTGCTTTGG ACATCACTAC GTCTGACCGC 
GACCGCAACA AGTATGGGTT GCTGGCGCGC CTCGCAGTGG AAGCCGGCTT CGACTGGGTC 
TACTACGAGT CCCGCAACCA CGTCCACGTG TCGGTCAAAG CTGATAACTC ACTGGCGGTC 
CGGGCGGGCG GCTGCTTTCC GGGAAATGCA ACTGTGCGCC TGTGGAGCGG CGAGCGGAAA 
GGGCTGCGGG AACTGCACCG CGGAGACTGG GTTTTGGCGG CCGATGCGTC AGGCCGGGTG 
GTGCCCACGC CGGTGCTGCT CTTCCTGGAC CGGGACTTGC AGCGCCGGGC TTCATTTGTG 
GCTGTGGAGA CCGAGTGGCC TCCACGCAAA CTGTTGCTCA CGCCCTGGCA CCTGGTGTTT 
GCCGCTCGAG GGCCGGCGCC CGCGCCAGGC GACTTTGCAC CGGTGTTCGC GCGCCGGCTA 
CGCGCTGGGG ACTCGGTGCT GGCGCCCGGC GGGGATGCGC TTCGGCCAGC GCGCGTGGCC 
CGTGTGGCGC GGGAGGAAGC CGTGGGCGTG TTCGCGCCGC TCACCGCGCA CGGGACGCTG 
CTGGTGAACG ATGTCCTGGC CTCTTGCTAC GCGGTTCTGG AGAGTCACCA GTGGGCGCAC 
CGCGCTTTTG CCCCCTTGAG ACTGCTGCAC GCGCTAGGGG CGCTGCTCCC CGGCGGGGCC 
GTCCAGCCGA CTGGCATGCA TTGGTACTCT CGGCTCCTCT ACCGCTTAGC GGAGGAGCTA 
CTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHH ( XM_017019380.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874869.1 Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu83827
Clone ID Related Accession (Same CDS sequence)	XM_017019380.1
Accession Version	XM_017019380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	desert hedgehog protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGCG CCAAGGTCTC CCGCTCCCAG GCTGGAGCGG CCAAGATAAC TGTGGTCTCA 
CTCCCCCAGG TGCAGGCTTC CAGCCGCAGC TTCTTTTCCT CTTCTCCGCT CTCACCCTGC 
AGGGGGCTCC CCAGATCCGC ACAAGGAGAC CGTTATTCTC AGCGTTGTAA GGAGCGGGTG 
AACGCTTTGG CCATTGCCGT GATGAACATG TGGCCCGGAG TGCGCCTACG AGTGACTGAG 
GGCTGGGACG AGGACGGCCA CCACGCTCAG GATTCACTCC ACTACGAAGG CCGTGCTTTG 
GACATCACTA CGTCTGACCG CGACCGCAAC AAGTATGGGT TGCTGGCGCG CCTCGCAGTG 
GAAGCCGGCT TCGACTGGGT CTACTACGAG TCCCGCAACC ACGTCCACGT GTCGGTCAAA 
GCTGATAACT CACTGGCGGT CCGGGCGGGC GGCTGCTTTC CGGGAAATGC AACTGTGCGC 
CTGTGGAGCG GCGAGCGGAA AGGGCTGCGG GAACTGCACC GCGGAGACTG GGTTTTGGCG 
GCCGATGCGT CAGGCCGGGT GGTGCCCACG CCGGTGCTGC TCTTCCTGGA CCGGGACTTG 
CAGCGCCGGG CTTCATTTGT GGCTGTGGAG ACCGAGTGGC CTCCACGCAA ACTGTTGCTC 
ACGCCCTGGC ACCTGGTGTT TGCCGCTCGA GGGCCGGCGC CCGCGCCAGG CGACTTTGCA 
CCGGTGTTCG CGCGCCGGCT ACGCGCTGGG GACTCGGTGC TGGCGCCCGG CGGGGATGCG 
CTTCGGCCAG CGCGCGTGGC CCGTGTGGCG CGGGAGGAAG CCGTGGGCGT GTTCGCGCCG 
CTCACCGCGC ACGGGACGCT GCTGGTGAAC GATGTCCTGG CCTCTTGCTA CGCGGTTCTG 
GAGAGTCACC AGTGGGCGCA CCGCGCTTTT GCCCCCTTGA GACTGCTGCA CGCGCTAGGG 
GCGCTGCTCC CCGGCGGGGC CGTCCAGCCG ACTGGCATGC ATTGGTACTC TCGGCTCCTC 
TACCGCTTAG CGGAGGAGCT ACTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874869.1
CDS	380..1429
Translation	MVRAKVSRSQAGAAKITVVSLPQVQASSRSFFSSSPLSPCRGLPRSAQGDRYSQRCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG

Target ORF information:

RefSeq Version	XM_017019380.1
Organism	Homo sapiens(human)
Definition	Homo sapiens desert hedgehog signaling molecule (DHH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019380.1

ORF Insert Sequence:

ATGGTGCGCG CCAAGGTCTC CCGCTCCCAG GCTGGAGCGG CCAAGATAAC TGTGGTCTCA 
CTCCCCCAGG TGCAGGCTTC CAGCCGCAGC TTCTTTTCCT CTTCTCCGCT CTCACCCTGC 
AGGGGGCTCC CCAGATCCGC ACAAGGAGAC CGTTATTCTC AGCGTTGTAA GGAGCGGGTG 
AACGCTTTGG CCATTGCCGT GATGAACATG TGGCCCGGAG TGCGCCTACG AGTGACTGAG 
GGCTGGGACG AGGACGGCCA CCACGCTCAG GATTCACTCC ACTACGAAGG CCGTGCTTTG 
GACATCACTA CGTCTGACCG CGACCGCAAC AAGTATGGGT TGCTGGCGCG CCTCGCAGTG 
GAAGCCGGCT TCGACTGGGT CTACTACGAG TCCCGCAACC ACGTCCACGT GTCGGTCAAA 
GCTGATAACT CACTGGCGGT CCGGGCGGGC GGCTGCTTTC CGGGAAATGC AACTGTGCGC 
CTGTGGAGCG GCGAGCGGAA AGGGCTGCGG GAACTGCACC GCGGAGACTG GGTTTTGGCG 
GCCGATGCGT CAGGCCGGGT GGTGCCCACG CCGGTGCTGC TCTTCCTGGA CCGGGACTTG 
CAGCGCCGGG CTTCATTTGT GGCTGTGGAG ACCGAGTGGC CTCCACGCAA ACTGTTGCTC 
ACGCCCTGGC ACCTGGTGTT TGCCGCTCGA GGGCCGGCGC CCGCGCCAGG CGACTTTGCA 
CCGGTGTTCG CGCGCCGGCT ACGCGCTGGG GACTCGGTGC TGGCGCCCGG CGGGGATGCG 
CTTCGGCCAG CGCGCGTGGC CCGTGTGGCG CGGGAGGAAG CCGTGGGCGT GTTCGCGCCG 
CTCACCGCGC ACGGGACGCT GCTGGTGAAC GATGTCCTGG CCTCTTGCTA CGCGGTTCTG 
GAGAGTCACC AGTGGGCGCA CCGCGCTTTT GCCCCCTTGA GACTGCTGCA CGCGCTAGGG 
GCGCTGCTCC CCGGCGGGGC CGTCCAGCCG ACTGGCATGC ATTGGTACTC TCGGCTCCTC 
TACCGCTTAG CGGAGGAGCT ACTGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel variant of DHH in a familial case of 46,XY disorder of sex development: Insights from molecular dynamics simulations.
Clinical endocrinology87(5)539-544(2017 Nov)
Paris F,Flatters D,Caburet S,Legois B,Servant N,Lefebvre H,Sultan C,Veitia RA

46,XY Gonadal Dysgenesis due to a Homozygous Mutation in Desert Hedgehog (DHH) Identified by Exome Sequencing.
The Journal of clinical endocrinology and metabolism100(7)E1022-9(2015 Jul)
Werner R,Merz H,Birnbaum W,Marshall L,Schr?der T,Reiz B,Kavran JM,B?umer T,Capetian P,Hiort O

A genetic variant in 12q13, a possible risk factor for bipolar disorder, is associated with depressive state, accounting for stressful life events.
PloS one9(12)e115135(2014)
Shimasaki A,Kondo K,Saito T,Esaki K,Otsuka Y,Mano K,Ikeda M,Iwata N

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DHH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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