TAS2R14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAS2R14
Entrez Gene ID	50840
Full Name	taste 2 receptor member 14
Synonyms	T2R14,TRB1
General protein information	Preferred Names taste 2 receptor member 14 Names taste receptor type 2 member 14 taste receptor, family B, member 1 taste receptor, type 2, member 14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.2
Summary	This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13. [provided by RefSeq, Jul 2008].
Disorder MIM:	604790

mRNA and Protein(s)

mRNA	Protein	Name
NM_023922.1	NP_076411.1	taste receptor type 2 member 14

Pathways from BioSystems

Homologies

	LOC718111	XP_001115534.1
Pan troglodytes (chimpanzee)	TAS2R14	XP_001151404.2
Homo sapiens (human)	TAS2R14	NP_076411.1
Rattus norvegicus (Norway rat)	Tas2r140	NP_001078866.1
Mus musculus (house mouse)	Tas2r140	NP_067537.1

Gene Ontology
Bibliography

Related articles in PubMed

Taste receptor polymorphisms and male infertility.
Gentiluomo M, Crifasi L, Luddi A, Locci D, Barale R, Piomboni P, Campa D
Human reproduction (Oxford, England)32(11)2324-2331(2017 Nov)

Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via G
Kim D, Woo JA, Geffken E, An SS, Liggett SB
American journal of respiratory cell and molecular biology56(6)762-771(2017 Jun)

Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor.
Hariri BM, McMahon DB, Chen B, Freund JR, Mansfield CJ, Doghramji LJ, Adappa ND, Palmer JN, Kennedy DW, Reed DR, Jiang P, Lee RJ
The Journal of biological chemistry292(20)8484-8497(2017 05)

?2-Adrenergic Receptors Chaperone Trapped Bitter Taste Receptor 14 to the Cell Surface as a Heterodimer and Exert Unidirectional Desensitization of Taste Receptor Function.
Kim D, Pauer SH, Yong HM, An SS, Liggett SB
The Journal of biological chemistry291(34)17616-28(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that, unlike in taste cells, TAS2Rs couple to the prevalent G proteins, Galphai1, Galphai2, and Galphai3, with no evidence for functional coupling to Galphagust.
Title: Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via G

Heterologously expressed T2R14 responds to multiple flavones. These flavones also activate T2R14-driven calcium signals in primary cells that activate nitric oxide production to increase ciliary beating and mucociliary clearance.
Title: Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor.

Thus the beta2AR acts as a double-edged sword: increasing TAS2R14 cell surface expression, but when activated by beta-agonist, partially offsetting the expression phenotype by direct receptor:receptor desensitization of TAS2R14 function.
Title: ?2-Adrenergic Receptors Chaperone Trapped Bitter Taste Receptor 14 to the Cell Surface as a Heterodimer and Exert Unidirectional Desensitization of Taste Receptor Function.

Probing the Binding Pocket of the Broadly Tuned Human Bitter Taste Receptor TAS2R14 by Chemical Modification of Cognate Agonists.
Title: Probing the Binding Pocket of the Broadly Tuned Human Bitter Taste Receptor TAS2R14 by Chemical Modification of Cognate Agonists.

TAS2R14 and TAS2R39 were activated by isoflavones and other isoflavonoids.
Title: Soy isoflavones and other isoflavonoids activate the human bitter taste receptors hTAS2R14 and hTAS2R39.

The following TAS2R14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAS2R14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu29269	NM_023922.1 Latest version!	Homo sapiens taste 2 receptor member 14 (TAS2R14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAS2R14 ( NM_023922.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_076411.1 Homo sapiens taste 2 receptor member 14 (TAS2R14), mRNA.

Price & Availability↑

CloneID	OHu29269
Clone ID Related Accession (Same CDS sequence)	NM_023922.1
Accession Version	NM_023922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	taste receptor type 2 member 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF227138.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC069148.1, BC101993.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGGTGGTG TCATAAAGAG CATATTTACA TTCGTTTTAA TTGTGGAATT TATAATTGGA 
AATTTAGGAA ATAGTTTCAT AGCACTGGTG AACTGTATTG ACTGGGTCAA GGGAAGAAAG 
ATCTCTTCGG TTGATCGGAT CCTCACTGCT TTGGCAATCT CTCGAATTAG CCTGGTTTGG 
TTAATATTCG GAAGCTGGTG TGTGTCTGTG TTTTTCCCAG CTTTATTTGC CACTGAAAAA 
ATGTTCAGAA TGCTTACTAA TATCTGGACA GTGATCAATC ATTTTAGTGT CTGGTTAGCT 
ACAGGCCTCG GTACTTTTTA TTTTCTCAAG ATAGCCAATT TTTCTAACTC TATTTTTCTC 
TACCTAAAGT GGAGGGTTAA AAAGGTGGTT TTGGTGCTGC TTCTTGTGAC TTCGGTCTTC 
TTGTTTTTAA ATATTGCACT GATAAACATC CATATAAATG CCAGTATCAA TGGATACAGA 
AGAAACAAGA CTTGCAGTTC TGATTCAAGT AACTTTACAC GATTTTCCAG TCTTATTGTA 
TTAACCAGCA CTGTGTTCAT TTTCATACCC TTTACTTTGT CCCTGGCAAT GTTTCTTCTC 
CTCATCTTCT CCATGTGGAA ACATCGCAAG AAGATGCAGC ACACTGTCAA AATATCCGGA 
GACGCCAGCA CCAAAGCCCA CAGAGGAGTT AAAAGTGTGA TCACTTTCTT CCTACTCTAT 
GCCATTTTCT CTCTGTCTTT TTTCATATCA GTTTGGACCT CTGAAAGGTT GGAGGAAAAT 
CTAATTATTC TTTCCCAGGT GATGGGAATG GCTTATCCTT CATGTCACTC ATGTGTTCTG 
ATTCTTGGAA ACAAGAAGCT GAGACAGGCC TCTCTGTCAG TGCTACTGTG GCTGAGGTAC 
ATGTTCAAAG ATGGGGAGCC CTCAGGTCAC AAAGAATTTA GAGAATCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076411.1
CDS	1..954
Translation	MGGVIKSIFTFVLIVEFIIGNLGNSFIALVNCIDWVKGRKISSVDRILTALAISRISLVWLIFGSWCVSVFFPALFATEKMFRMLTNIWTVINHFSVWLATGLGTFYFLKIANFSNSIFLYLKWRVKKVVLVLLLVTSVFLFLNIALINIHINASINGYRRNKTCSSDSSNFTRFSSLIVLTSTVFIFIPFTLSLAMFLLLIFSMWKHRKKMQHTVKISGDASTKAHRGVKSVITFFLLYAIFSLSFFISVWTSERLEENLIILSQVMGMAYPSCHSCVLILGNKKLRQASLSVLLWLRYMFKDGEPSGHKEFRESS

Target ORF information:

RefSeq Version	NM_023922.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 2 receptor member 14 (TAS2R14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023922.1

ORF Insert Sequence:

ATGGGTGGTG TCATAAAGAG CATATTTACA TTCGTTTTAA TTGTGGAATT TATAATTGGA 
AATTTAGGAA ATAGTTTCAT AGCACTGGTG AACTGTATTG ACTGGGTCAA GGGAAGAAAG 
ATCTCTTCGG TTGATCGGAT CCTCACTGCT TTGGCAATCT CTCGAATTAG CCTGGTTTGG 
TTAATATTCG GAAGCTGGTG TGTGTCTGTG TTTTTCCCAG CTTTATTTGC CACTGAAAAA 
ATGTTCAGAA TGCTTACTAA TATCTGGACA GTGATCAATC ATTTTAGTGT CTGGTTAGCT 
ACAGGCCTCG GTACTTTTTA TTTTCTCAAG ATAGCCAATT TTTCTAACTC TATTTTTCTC 
TACCTAAAGT GGAGGGTTAA AAAGGTGGTT TTGGTGCTGC TTCTTGTGAC TTCGGTCTTC 
TTGTTTTTAA ATATTGCACT GATAAACATC CATATAAATG CCAGTATCAA TGGATACAGA 
AGAAACAAGA CTTGCAGTTC TGATTCAAGT AACTTTACAC GATTTTCCAG TCTTATTGTA 
TTAACCAGCA CTGTGTTCAT TTTCATACCC TTTACTTTGT CCCTGGCAAT GTTTCTTCTC 
CTCATCTTCT CCATGTGGAA ACATCGCAAG AAGATGCAGC ACACTGTCAA AATATCCGGA 
GACGCCAGCA CCAAAGCCCA CAGAGGAGTT AAAAGTGTGA TCACTTTCTT CCTACTCTAT 
GCCATTTTCT CTCTGTCTTT TTTCATATCA GTTTGGACCT CTGAAAGGTT GGAGGAAAAT 
CTAATTATTC TTTCCCAGGT GATGGGAATG GCTTATCCTT CATGTCACTC ATGTGTTCTG 
ATTCTTGGAA ACAAGAAGCT GAGACAGGCC TCTCTGTCAG TGCTACTGTG GCTGAGGTAC 
ATGTTCAAAG ATGGGGAGCC CTCAGGTCAC AAAGAATTTA GAGAATCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Taste receptor polymorphisms and male infertility.
Human reproduction (Oxford, England)32(11)2324-2331(2017 Nov)
Gentiluomo M,Crifasi L,Luddi A,Locci D,Barale R,Piomboni P,Campa D

Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via G
American journal of respiratory cell and molecular biology56(6)762-771(2017 Jun)
Kim D,Woo JA,Geffken E,An SS,Liggett SB

Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor.
The Journal of biological chemistry292(20)8484-8497(2017 05)
Hariri BM,McMahon DB,Chen B,Freund JR,Mansfield CJ,Doghramji LJ,Adappa ND,Palmer JN,Kennedy DW,Reed DR,Jiang P,Lee RJ

?2-Adrenergic Receptors Chaperone Trapped Bitter Taste Receptor 14 to the Cell Surface as a Heterodimer and Exert Unidirectional Desensitization of Taste Receptor Function.
The Journal of biological chemistry291(34)17616-28(2016 08)
Kim D,Pauer SH,Yong HM,An SS,Liggett SB

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TAS2R14 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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