DCAF8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCAF8
Entrez Gene ID	50717
Full Name	DDB1 and CUL4 associated factor 8
Synonyms	GAN2,H326,WDR42A
General protein information	Preferred Names DDB1 and CUL4 associated factor 8 Names DDB1- and CUL4-associated factor 8 WD repeat domain 42A WD repeat-containing protein 42A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.2
Summary	This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009].
Disorder MIM:	615820
Disorder Html:	?Giant axonal neuropathy 2, autosomal dominant, 610100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015726.4	NP_056541.2	DDB1- and CUL4-associated factor 8
NM_015726.3	NP_056541.2	DDB1- and CUL4-associated factor 8

Pathways from BioSystems

Homologies

	LOC719645	XP_002808293.1
Xenopus tropicalis (tropical clawed frog)	dcaf8	NP_001016610.1
Homo sapiens (human)	DCAF8	NP_056541.2
Canis lupus familiaris (dog)	DCAF8	XP_536129.2
Bos taurus (cattle)	DCAF8	NP_001193348.1
Rattus norvegicus (Norway rat)	Dcaf8	NP_001014253.1
Gallus gallus (chicken)	DCAF8	XP_426809.4
Arabidopsis thaliana (thale cress)	AT3G45620	NP_001190014.1
Pan troglodytes (chimpanzee)	DCAF8	XP_003308591.1
Oryza sativa (rice)	Os03g0286800	NP_001049775.1
Mus musculus (house mouse)	Dcaf8	NP_705783.1
Danio rerio (zebrafish)	LOC100007924	XP_001346262.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons.
Klein CJ, Wu Y, Vogel P, Goebel HH, B?nnemann C, Zukosky K, Botuyan MV, Duan X, Middha S, Atkinson EJ, Mer G, Dyck PJ
Neurology82(10)873-8(2014 Mar)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A).
Wu F, Wang S, Xing J, Li M, Zheng C
FEBS letters586(8)1079-85(2012 Apr)

Global landscape of HIV-human protein complexes.
J?ger S, Cimermancic P, Gulbahce N, Johnson JR, McGovern KE, Clarke SC, Shales M, Mercenne G, Pache L, Li K, Hernandez H, Jang GM, Roth SL, Akiva E, Marlett J, Stephens M, D'Orso I, Fernandes J, Fahey M, Mahon C, O'Donoghue AJ, Todorovic A, Morris JH, Maltby DA, Alber T, Cagney G, Bushman FD, Young JA, Chanda SK, Sundquist WI, Kortemme T, Hernandez RD, Craik CS, Burlingame A, Sali A, Frankel AD, Krogan NJ
Nature481(7381)365-70(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DCAF8 p.R317C mutation is responsible for specific variety of HMSN2 with infrequent giant axons and mild cardiomyopathy.
Title: Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons.

WDR42A is a nucleocytoplasmic shuttling protein.
Title: Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A).

while RNF10 and WDR42A or VP22 alone showed distinct subcellular localization patterns, RNF10 and WDR42A were relocated when co-expressed with VP22 or its homologues; these potential host cell factors of VP22 might expand the list of host targets of VP22
Title: Host cell targets of tegument protein VP22 of herpes simplex virus 1.

The following DCAF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCAF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27915	NM_015726.4 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu27915	NM_015726.3	Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCAF8 ( NM_015726.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056541.2 Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27915
Clone ID Related Accession (Same CDS sequence)	NM_015726.4 , NM_015726.3
Accession Version	NM_015726.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139011.20. On Jun 2, 2019 this sequence version replaced NM_015726.3. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.216733.1, SRR1803614.48999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGCA AAGGGAGCAG CACAGATGGC AGAACAGACT TAGCTAATGG AAGCCTGTCT 
AGCAGTCCAG AGGAGATGTC TGGAGCTGAA GAGGGGAGGG AGACATCCTC AGGCATTGAA 
GTGGAGGCCT CAGACCTGAG TTTGAGCTTG ACTGGGGATG ATGGTGGCCC CAACCGCACC 
AGCACAGAAA GTCGAGGCAC AGACACAGAG AGCTCAGGTG AAGATAAGGA CTCTGACAGC 
ATGGAGGACA CTGGTCATTA CTCCATTAAT GATGAAAATC GAGTCCATGA CCGCTCAGAG 
GAAGAGGAAG AGGAGGAAGA AGAGGAGGAA GAAGAGCAGC CTCGGCGCCG TGTACAGCGC 
AAGCGGGCTA ACCGTGACCA GGACTCATCA GATGATGAGC GGGCCCTAGA GGACTGGGTG 
TCCTCAGAAA CATCAGCTCT ACCCCGACCT CGCTGGCAAG CCCTCCCTGC CCTTCGGGAG 
CGGGAGCTGG GTTCAAGTGC CCGCTTTGTC TATGAGGCCT GTGGGGCAAG AGTCTTTGTG 
CAGCGTTTCC GCCTGCAGCA TGGGCTTGAG GGCCATACTG GTTGTGTCAA TACCCTGCAC 
TTTAACCAGC GCGGCACCTG GCTGGCCAGT GGCAGCGATG ACCTGAAGGT GGTGGTGTGG 
GATTGGGTAC GGCGGCAGCC AGTACTGGAC TTTGAGAGTG GCCACAAAAG TAATGTGTTC 
CAGGCCAAGT TTCTTCCTAA CAGTGGTGAT TCTACTCTGG CCATGTGTGC CCGTGACGGG 
CAGGTTCGAG TAGCAGAACT GTCTGCCACA CAGTGTTGCA AGAATACAAA ACGTGTGGCC 
CAGCACAAGG GAGCGTCCCA CAAGTTGGCA CTGGAACCAG ACTCTCCCTG TACGTTCTTA 
TCTGCAGGTG AAGATGCAGT TGTTTTCACC ATTGACCTGA GACAAGACCG CCCAGCGTCG 
AAACTGGTGG TGACAAAAGA GAAAGAGAAG AAAGTGGGGC TGTATACGAT CTATGTGAAT 
CCTGCCAATA CCCACCAGTT TGCAGTGGGT GGACGAGATC AGTTTGTAAG GATTTATGAC 
CAGAGGAAAA TTGATGAGAA TGAGAACAAT GGAGTACTCA AGAAGTTCTG TCCTCATCAC 
CTGGTGAACA GTGAGTCCAA AGCAAACATC ACCTGTCTTG TGTACAGCCA CGACGGCACA 
GAGCTCCTGG CCAGTTACAA TGATGAAGAC ATTTACCTCT TCAACTCCTC TCACAGTGAT 
GGGGCCCAGT ATGTTAAGAG ATACAAGGGC CACAGAAATA ATGCCACAGT AAAAGGCGTC 
AATTTCTATG GCCCCAAGAG TGAGTTTGTG GTGAGCGGTA GTGACTGTGG GCACATCTTC 
CTCTGGGAGA AATCATCCTG CCAGATTATT CAGTTCATGG AGGGGGACAA GGGAGGCGTG 
GTAAACTGTC TTGAGCCCCA CCCTCACCTG CCTGTGCTGG CAACCAGTGG CCTAGACCAT 
GATGTGAAGA TCTGGGCACC CACAGCTGAA GCTTCCACTG AGCTGACAGG GTTAAAAGAT 
GTGATTAAGA AGAACAAGCG GGAGCGGGAT GAAGATAGCT TGCACCAAAC TGACCTGTTT 
GATAGTCACA TGCTGTGGTT CCTTATGCAT CACCTGAGAC AGAGACGCCA TCACCGGCGC 
TGGCGAGAAC CTGGGGTTGG GGCCACAGAC GCGGACTCTG ATGAGTCTCC CAGCTCCTCA 
GACACATCGG ACGAGGAGGA GGGCCCTGAC CGGGTGCAGT GCATGCCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056541.2
CDS	202..1995
Translation	MSSKGSSTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEDKDSDSMEDTGHYSINDENRVHDRSEEEEEEEEEEEEEQPRRRVQRKRANRDQDSSDDERALEDWVSSETSALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	NM_015726.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015726.4

ORF Insert Sequence:

ATGTCCAGCA AAGGGAGCAG CACAGATGGC AGAACAGACT TAGCTAATGG AAGCCTGTCT 
AGCAGTCCAG AGGAGATGTC TGGAGCTGAA GAGGGGAGGG AGACATCCTC AGGCATTGAA 
GTGGAGGCCT CAGACCTGAG TTTGAGCTTG ACTGGGGATG ATGGTGGCCC CAACCGCACC 
AGCACAGAAA GTCGAGGCAC AGACACAGAG AGCTCAGGTG AAGATAAGGA CTCTGACAGC 
ATGGAGGACA CTGGTCATTA CTCCATTAAT GATGAAAATC GAGTCCATGA CCGCTCAGAG 
GAAGAGGAAG AGGAGGAAGA AGAGGAGGAA GAAGAGCAGC CTCGGCGCCG TGTACAGCGC 
AAGCGGGCTA ACCGTGACCA GGACTCATCA GATGATGAGC GGGCCCTAGA GGACTGGGTG 
TCCTCAGAAA CATCAGCTCT ACCCCGACCT CGCTGGCAAG CCCTCCCTGC CCTTCGGGAG 
CGGGAGCTGG GTTCAAGTGC CCGCTTTGTC TATGAGGCCT GTGGGGCAAG AGTCTTTGTG 
CAGCGTTTCC GCCTGCAGCA TGGGCTTGAG GGCCATACTG GTTGTGTCAA TACCCTGCAC 
TTTAACCAGC GCGGCACCTG GCTGGCCAGT GGCAGCGATG ACCTGAAGGT GGTGGTGTGG 
GATTGGGTAC GGCGGCAGCC AGTACTGGAC TTTGAGAGTG GCCACAAAAG TAATGTGTTC 
CAGGCCAAGT TTCTTCCTAA CAGTGGTGAT TCTACTCTGG CCATGTGTGC CCGTGACGGG 
CAGGTTCGAG TAGCAGAACT GTCTGCCACA CAGTGTTGCA AGAATACAAA ACGTGTGGCC 
CAGCACAAGG GAGCGTCCCA CAAGTTGGCA CTGGAACCAG ACTCTCCCTG TACGTTCTTA 
TCTGCAGGTG AAGATGCAGT TGTTTTCACC ATTGACCTGA GACAAGACCG CCCAGCGTCG 
AAACTGGTGG TGACAAAAGA GAAAGAGAAG AAAGTGGGGC TGTATACGAT CTATGTGAAT 
CCTGCCAATA CCCACCAGTT TGCAGTGGGT GGACGAGATC AGTTTGTAAG GATTTATGAC 
CAGAGGAAAA TTGATGAGAA TGAGAACAAT GGAGTACTCA AGAAGTTCTG TCCTCATCAC 
CTGGTGAACA GTGAGTCCAA AGCAAACATC ACCTGTCTTG TGTACAGCCA CGACGGCACA 
GAGCTCCTGG CCAGTTACAA TGATGAAGAC ATTTACCTCT TCAACTCCTC TCACAGTGAT 
GGGGCCCAGT ATGTTAAGAG ATACAAGGGC CACAGAAATA ATGCCACAGT AAAAGGCGTC 
AATTTCTATG GCCCCAAGAG TGAGTTTGTG GTGAGCGGTA GTGACTGTGG GCACATCTTC 
CTCTGGGAGA AATCATCCTG CCAGATTATT CAGTTCATGG AGGGGGACAA GGGAGGCGTG 
GTAAACTGTC TTGAGCCCCA CCCTCACCTG CCTGTGCTGG CAACCAGTGG CCTAGACCAT 
GATGTGAAGA TCTGGGCACC CACAGCTGAA GCTTCCACTG AGCTGACAGG GTTAAAAGAT 
GTGATTAAGA AGAACAAGCG GGAGCGGGAT GAAGATAGCT TGCACCAAAC TGACCTGTTT 
GATAGTCACA TGCTGTGGTT CCTTATGCAT CACCTGAGAC AGAGACGCCA TCACCGGCGC 
TGGCGAGAAC CTGGGGTTGG GGCCACAGAC GCGGACTCTG ATGAGTCTCC CAGCTCCTCA 
GACACATCGG ACGAGGAGGA GGGCCCTGAC CGGGTGCAGT GCATGCCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF8 ( NM_015726.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056541.2 Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27915
Clone ID Related Accession (Same CDS sequence)	NM_015726.4 , NM_015726.3
Accession Version	NM_015726.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA622120.1, AK303188.1, BC099709.1, AL139011.20 and BU629460.1. This sequence is a reference standard in the RefSeqGene project. On Jul 22, 2009 this sequence version replaced NM_015726.2. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.216733.1, SRR1803616.69461.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGCA AAGGGAGCAG CACAGATGGC AGAACAGACT TAGCTAATGG AAGCCTGTCT 
AGCAGTCCAG AGGAGATGTC TGGAGCTGAA GAGGGGAGGG AGACATCCTC AGGCATTGAA 
GTGGAGGCCT CAGACCTGAG TTTGAGCTTG ACTGGGGATG ATGGTGGCCC CAACCGCACC 
AGCACAGAAA GTCGAGGCAC AGACACAGAG AGCTCAGGTG AAGATAAGGA CTCTGACAGC 
ATGGAGGACA CTGGTCATTA CTCCATTAAT GATGAAAATC GAGTCCATGA CCGCTCAGAG 
GAAGAGGAAG AGGAGGAAGA AGAGGAGGAA GAAGAGCAGC CTCGGCGCCG TGTACAGCGC 
AAGCGGGCTA ACCGTGACCA GGACTCATCA GATGATGAGC GGGCCCTAGA GGACTGGGTG 
TCCTCAGAAA CATCAGCTCT ACCCCGACCT CGCTGGCAAG CCCTCCCTGC CCTTCGGGAG 
CGGGAGCTGG GTTCAAGTGC CCGCTTTGTC TATGAGGCCT GTGGGGCAAG AGTCTTTGTG 
CAGCGTTTCC GCCTGCAGCA TGGGCTTGAG GGCCATACTG GTTGTGTCAA TACCCTGCAC 
TTTAACCAGC GCGGCACCTG GCTGGCCAGT GGCAGCGATG ACCTGAAGGT GGTGGTGTGG 
GATTGGGTAC GGCGGCAGCC AGTACTGGAC TTTGAGAGTG GCCACAAAAG TAATGTGTTC 
CAGGCCAAGT TTCTTCCTAA CAGTGGTGAT TCTACTCTGG CCATGTGTGC CCGTGACGGG 
CAGGTTCGAG TAGCAGAACT GTCTGCCACA CAGTGTTGCA AGAATACAAA ACGTGTGGCC 
CAGCACAAGG GAGCGTCCCA CAAGTTGGCA CTGGAACCAG ACTCTCCCTG TACGTTCTTA 
TCTGCAGGTG AAGATGCAGT TGTTTTCACC ATTGACCTGA GACAAGACCG CCCAGCGTCG 
AAACTGGTGG TGACAAAAGA GAAAGAGAAG AAAGTGGGGC TGTATACGAT CTATGTGAAT 
CCTGCCAATA CCCACCAGTT TGCAGTGGGT GGACGAGATC AGTTTGTAAG GATTTATGAC 
CAGAGGAAAA TTGATGAGAA TGAGAACAAT GGAGTACTCA AGAAGTTCTG TCCTCATCAC 
CTGGTGAACA GTGAGTCCAA AGCAAACATC ACCTGTCTTG TGTACAGCCA CGACGGCACA 
GAGCTCCTGG CCAGTTACAA TGATGAAGAC ATTTACCTCT TCAACTCCTC TCACAGTGAT 
GGGGCCCAGT ATGTTAAGAG ATACAAGGGC CACAGAAATA ATGCCACAGT AAAAGGCGTC 
AATTTCTATG GCCCCAAGAG TGAGTTTGTG GTGAGCGGTA GTGACTGTGG GCACATCTTC 
CTCTGGGAGA AATCATCCTG CCAGATTATT CAGTTCATGG AGGGGGACAA GGGAGGCGTG 
GTAAACTGTC TTGAGCCCCA CCCTCACCTG CCTGTGCTGG CAACCAGTGG CCTAGACCAT 
GATGTGAAGA TCTGGGCACC CACAGCTGAA GCTTCCACTG AGCTGACAGG GTTAAAAGAT 
GTGATTAAGA AGAACAAGCG GGAGCGGGAT GAAGATAGCT TGCACCAAAC TGACCTGTTT 
GATAGTCACA TGCTGTGGTT CCTTATGCAT CACCTGAGAC AGAGACGCCA TCACCGGCGC 
TGGCGAGAAC CTGGGGTTGG GGCCACAGAC GCGGACTCTG ATGAGTCTCC CAGCTCCTCA 
GACACATCGG ACGAGGAGGA GGGCCCTGAC CGGGTGCAGT GCATGCCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056541.2
CDS	213..2006
Translation	MSSKGSSTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEDKDSDSMEDTGHYSINDENRVHDRSEEEEEEEEEEEEEQPRRRVQRKRANRDQDSSDDERALEDWVSSETSALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	NM_015726.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015726.3

ORF Insert Sequence:

ATGTCCAGCA AAGGGAGCAG CACAGATGGC AGAACAGACT TAGCTAATGG AAGCCTGTCT 
AGCAGTCCAG AGGAGATGTC TGGAGCTGAA GAGGGGAGGG AGACATCCTC AGGCATTGAA 
GTGGAGGCCT CAGACCTGAG TTTGAGCTTG ACTGGGGATG ATGGTGGCCC CAACCGCACC 
AGCACAGAAA GTCGAGGCAC AGACACAGAG AGCTCAGGTG AAGATAAGGA CTCTGACAGC 
ATGGAGGACA CTGGTCATTA CTCCATTAAT GATGAAAATC GAGTCCATGA CCGCTCAGAG 
GAAGAGGAAG AGGAGGAAGA AGAGGAGGAA GAAGAGCAGC CTCGGCGCCG TGTACAGCGC 
AAGCGGGCTA ACCGTGACCA GGACTCATCA GATGATGAGC GGGCCCTAGA GGACTGGGTG 
TCCTCAGAAA CATCAGCTCT ACCCCGACCT CGCTGGCAAG CCCTCCCTGC CCTTCGGGAG 
CGGGAGCTGG GTTCAAGTGC CCGCTTTGTC TATGAGGCCT GTGGGGCAAG AGTCTTTGTG 
CAGCGTTTCC GCCTGCAGCA TGGGCTTGAG GGCCATACTG GTTGTGTCAA TACCCTGCAC 
TTTAACCAGC GCGGCACCTG GCTGGCCAGT GGCAGCGATG ACCTGAAGGT GGTGGTGTGG 
GATTGGGTAC GGCGGCAGCC AGTACTGGAC TTTGAGAGTG GCCACAAAAG TAATGTGTTC 
CAGGCCAAGT TTCTTCCTAA CAGTGGTGAT TCTACTCTGG CCATGTGTGC CCGTGACGGG 
CAGGTTCGAG TAGCAGAACT GTCTGCCACA CAGTGTTGCA AGAATACAAA ACGTGTGGCC 
CAGCACAAGG GAGCGTCCCA CAAGTTGGCA CTGGAACCAG ACTCTCCCTG TACGTTCTTA 
TCTGCAGGTG AAGATGCAGT TGTTTTCACC ATTGACCTGA GACAAGACCG CCCAGCGTCG 
AAACTGGTGG TGACAAAAGA GAAAGAGAAG AAAGTGGGGC TGTATACGAT CTATGTGAAT 
CCTGCCAATA CCCACCAGTT TGCAGTGGGT GGACGAGATC AGTTTGTAAG GATTTATGAC 
CAGAGGAAAA TTGATGAGAA TGAGAACAAT GGAGTACTCA AGAAGTTCTG TCCTCATCAC 
CTGGTGAACA GTGAGTCCAA AGCAAACATC ACCTGTCTTG TGTACAGCCA CGACGGCACA 
GAGCTCCTGG CCAGTTACAA TGATGAAGAC ATTTACCTCT TCAACTCCTC TCACAGTGAT 
GGGGCCCAGT ATGTTAAGAG ATACAAGGGC CACAGAAATA ATGCCACAGT AAAAGGCGTC 
AATTTCTATG GCCCCAAGAG TGAGTTTGTG GTGAGCGGTA GTGACTGTGG GCACATCTTC 
CTCTGGGAGA AATCATCCTG CCAGATTATT CAGTTCATGG AGGGGGACAA GGGAGGCGTG 
GTAAACTGTC TTGAGCCCCA CCCTCACCTG CCTGTGCTGG CAACCAGTGG CCTAGACCAT 
GATGTGAAGA TCTGGGCACC CACAGCTGAA GCTTCCACTG AGCTGACAGG GTTAAAAGAT 
GTGATTAAGA AGAACAAGCG GGAGCGGGAT GAAGATAGCT TGCACCAAAC TGACCTGTTT 
GATAGTCACA TGCTGTGGTT CCTTATGCAT CACCTGAGAC AGAGACGCCA TCACCGGCGC 
TGGCGAGAAC CTGGGGTTGG GGCCACAGAC GCGGACTCTG ATGAGTCTCC CAGCTCCTCA 
GACACATCGG ACGAGGAGGA GGGCCCTGAC CGGGTGCAGT GCATGCCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons.
Neurology82(10)873-8(2014 Mar)
Klein CJ,Wu Y,Vogel P,Goebel HH,B?nnemann C,Zukosky K,Botuyan MV,Duan X,Middha S,Atkinson EJ,Mer G,Dyck PJ

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A).
FEBS letters586(8)1079-85(2012 Apr)
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DCAF8 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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