ITSN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITSN2
Entrez Gene ID	50618
Full Name	intersectin 2
Synonyms	PRO2015,SH3D1B,SH3P18,SWA,SWAP
General protein information	Preferred Names intersectin 2 Names intersectin-2 SH3 domain-containing protein 1B SH3P18-like WASP-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017].
Disorder MIM:	604464

mRNA and Protein(s)

mRNA	Protein	Name
NM_147152.2	NP_671494.2	intersectin-2 isoform 2
NM_001348185.1	NP_001335114.1	intersectin-2 isoform 8
XM_024452930.1	XP_024308698.1	intersectin-2 isoform X1
XM_024452931.1	XP_024308699.1	intersectin-2 isoform X1
XM_024452937.1	XP_024308705.1	intersectin-2 isoform X7
NM_019595.3	NP_062541.3	intersectin-2 isoform 3
NM_001348184.1	NP_001335113.1	intersectin-2 isoform 7
NM_001348186.1	NP_001335115.1	intersectin-2 isoform 9
NM_001348184.2	NP_001335113.1	intersectin-2 isoform 7
NM_006277.2	NP_006268.2	intersectin-2 isoform 1
NM_001348186.2	NP_001335115.1	intersectin-2 isoform 9
XM_024452932.1	XP_024308700.1	intersectin-2 isoform X2
XM_024452933.1	XP_024308701.1	intersectin-2 isoform X3
NM_006277.3	NP_006268.2	intersectin-2 isoform 1
NM_001348182.1	NP_001335111.1	intersectin-2 isoform 5
NM_001348183.1	NP_001335112.1	intersectin-2 isoform 6
NM_001348181.1	NP_001335110.1	intersectin-2 isoform 4
NM_001348183.2	NP_001335112.1	intersectin-2 isoform 6
NM_001348181.2	NP_001335110.1	intersectin-2 isoform 4
NM_001348182.2	NP_001335111.1	intersectin-2 isoform 5
XM_024452934.1	XP_024308702.1	intersectin-2 isoform X4
XM_024452935.1	XP_024308703.1	intersectin-2 isoform X5
XM_024452936.1	XP_024308704.1	intersectin-2 isoform X6
NM_019595.4	NP_062541.3	intersectin-2 isoform 3
NM_001348185.2	NP_001335114.1	intersectin-2 isoform 8
NM_147152.3	NP_671494.2	intersectin-2 isoform 2

Pathways from BioSystems

Homologies

	LOC704858	XP_002808077.1
Rattus norvegicus (Norway rat)	Itsn2	XP_003750174.1
Mus musculus (house mouse)	Itsn2	NP_001185897.1
Gallus gallus (chicken)	ITSN2	XP_419989.4
Pan troglodytes (chimpanzee)	ITSN2	XP_001145237.3
Canis lupus familiaris (dog)	ITSN2	XP_849291.2
Danio rerio (zebrafish)	itsn2a	NP_001138272.1
Xenopus tropicalis (tropical clawed frog)	itsn2	XP_004918242.1
Homo sapiens (human)	ITSN2	NP_062541.3
Bos taurus (cattle)	ITSN2	XP_002691512.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.
Kropyvko S, Gryaznova T, Morderer D, Rynditch A
Biochemical and biophysical research communications484(4)813-819(2017 03)

ITSN2L Interacts with and Negatively Regulates RABEP1.
Yang X, Yan F, He Z, Liu S, Cheng Y, Wei K, Gan S, Yuan J, Wang S, Xiao Y, Ren K, Liu N, Hu X, Ding X, Hu X, Xiang S
International journal of molecular sciences16(12)28242-54(2015 Nov)

Genome-wide association study identifies multiple susceptibility loci for diffuse large B cell lymphoma.
Cerhan JR, Berndt SI, Vijai J, Ghesqui?res H, McKay J, Wang SS, Wang Z, Yeager M, Conde L, de Bakker PI, Nieters A, Cox D, Burdett L, Monnereau A, Flowers CR, De Roos AJ, Brooks-Wilson AR, Lan Q, Severi G, Melbye M, Gu J, Jackson RD, Kane E, Teras LR, Purdue MP, Vajdic CM, Spinelli JJ, Giles GG, Albanes D, Kelly RS, Zucca M, Bertrand KA, Zeleniuch-Jacquotte A, Lawrence C, Hutchinson A, Zhi D, Habermann TM, Link BK, Novak AJ, Dogan A, Asmann YW, Liebow M, Thompson CA, Ansell SM, Witzig TE, Weiner GJ, Veron AS, Zelenika D, Tilly H, Haioun C, Molina TJ, Hjalgrim H, Glimelius B, Adami HO, Bracci PM, Riby J, Smith MT, Holly EA, Cozen W, Hartge P, Morton LM, Severson RK, Tinker LF, North KE, Becker N, Benavente Y, Boffetta P, Brennan P, Foretova L, Maynadie M, Staines A, Lightfoot T, Crouch S, Smith A, Roman E, Diver WR, Offit K, Zelenetz A, Klein RJ, Villano DJ, Zheng T, Zhang Y, Holford TR, Kricker A, Turner J, Southey MC, Clavel J, Virtamo J, Weinstein S, Riboli E, Vineis P, Kaaks R, Trichopoulos D, Vermeulen RC, Boeing H, Tjonneland A, Angelucci E, Di Lollo S, Rais M, Birmann BM, Laden F, Giovannucci E, Kraft P, Huang J, Ma B, Ye Y, Chiu BC, Sampson J, Liang L, Park JH, Chung CC, Weisenburger DD, Chatterjee N, Fraumeni JF Jr, Slager SL, Wu X, de Sanjose S, Smedby KE, Salles G, Skibola CF, Rothman N, Chanock SJ
Nature genetics46(11)1233-8(2014 Nov)

SYT14L, especially its C2 domain, is involved in regulating melanocyte differentiation.
Yoo JC, Lim Ty, Park JS, Hah YS, Park N, Hong SG, Park JY, Yoon TJ
Journal of dermatological science72(3)246-51(2013 Dec)

Adaptor proteins intersectin 1 and 2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins.
Novokhatska O, Dergai M, Tsyba L, Skrypkina I, Filonenko V, Moreau J, Rynditch A
PloS one8(7)e70546(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion.
Title: Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.

ITSN2L interacts with RABEP1 and stimulates its degradation in regulation of endocytosis.
Title: ITSN2L Interacts with and Negatively Regulates RABEP1.

The sequence and structural alignment of ITSN-2L C2 domain with other members of C2 domain protein family indicate its vital cellular roles in membrane trafficking, the generation of lipid-second messengers and activation of GTPases. Moreover, our data show the possible roles of ITSN-2L C2 domain in regulating the activity of Cdc42.
Title: Crystal structure of human Intersectin-2L C2 domain.

ITSN1 and ITSN2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins.
Title: Adaptor proteins intersectin 1 and 2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins.

The ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins.
Title: Human intersectin 2 (ITSN2) binds to Eps8 protein and enhances its degradation.

The following ITSN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18937	NM_006277.3 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu104907	XM_024452937.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100853	NM_001348183.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100854	NM_001348186.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100855	NM_001348185.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu100857	NM_001348184.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu104906	XM_024452936.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu10175	NM_147152.3 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$881.30 $1259.00
OHu100852	NM_001348181.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu100856	NM_001348182.2 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu100852	XM_024452932.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104904	XM_024452933.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104905	XM_024452935.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu10176	NM_019595.4 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu10176	XM_024452934.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu18937	XM_024452931.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu18937	XM_024452930.1 Latest version!	Homo sapiens intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu100857	NM_001348184.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100854	NM_001348186.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu18937	NM_006277.2	Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu100853	NM_001348183.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100852	NM_001348181.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu100856	NM_001348182.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu10176	NM_019595.3	Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu100855	NM_001348185.1	Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu10175	NM_147152.2	Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITSN2 ( NM_006277.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006268.2 Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18937
Clone ID Related Accession (Same CDS sequence)	XM_024452930.1 , XM_024452931.1 , NM_006277.2 , NM_006277.3
Accession Version	NM_006277.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4, DA382952.1, AF182198.1, BC146779.1 and AC008073.4. This sequence is a reference standard in the RefSeqGene project. On Jun 3, 2019 this sequence version replaced NM_006277.2. Transcript Variant: This variant (1), also known as ITSN2-L, encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006268.2
CDS	267..5360
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_006277.3
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006277.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452937.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308705.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X9, mRNA.

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CloneID	OHu104907
Clone ID Related Accession (Same CDS sequence)	XM_024452937.1
Accession Version	XM_024452937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG GATTGGAGAA CAGAGACACA ACTACCATAT TCATTTATTC AGTTGATGAA 
AAAACCGTAG GAGAACCTGG TTGGCTTTAT GGTAGTTTTC AAGGAAATTT TGGCTGGTTT 
CCATGCAATT ATGTAGAAAA AATGCCATCA AGTGAAAATG AAAAAGCTGT ATCTCCAAAG 
AAGGCCTTAC TTCCTCCTAC AGTTTCTTTA TCTGCTACCT CAACTTCCTC TGAACCACTT 
TCTTCAAATC AACCAGCATC AGTGACTGAT TATCAAAATG TATCTTTTTC AAACCTAACT 
GTAAATACAT CATGGCAGAA AAAATCAGCC TTCACTCGAA CTGTGTCCCC TGGATCTGTA 
TCACCTATTC ATGGACAGGG ACAAGTGGTA GAAAACTTAA AAGCACAGGC CCTTTGTTCC 
TGGACTGCAA AGAAAGATAA CCACTTGAAC TTCTCAAAAC ATGACATTAT TACTGTCTTG 
GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTGCATGGAG GAAGAGGATG GTTTCCCAAA 
TCTTATGTCA AGATCATTCC TGGGAGTGAA GTAAAACGGG AAGAACCAGA AGCTTTGTAT 
GCAGCTGTAA ATAAGAAACC TACCTCGGCA GCCTATTCAG TTGGAGAAGA ATATATTGCA 
CTTTATCCAT ATTCAAGTGT GGAACCTGGA GATTTGACTT TCACAGAAGG TGAAGAAATA 
TTGGTGACCC AGAAAGATGG AGAGTGGTGG ACAGGAAGTA TTGGAGATAG AAGTGGAATT 
TTTCCATCAA ACTATGTCAA ACCAAAGGAT CAAGAGAGTT TTGGGAGTGC TAGCAAGTCT 
GGAGCATCAA ATAAAAAACC TGAGATTGCT CAGGTAACTT CAGCATATGT TGCTTCTGGT 
TCTGAACAAC TTAGCCTTGC ACCAGGACAG TTAATATTAA TTCTAAAGAA AAATACAAGT 
GGGTGGTGGC AAGGAGAGTT ACAGGCCAGA GGAAAAAAGC GACAGAAAGG ATGGTTTCCT 
GCCAGTCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAG CCACACCTGC CTTTCATCCT 
GTATGTCAGG TGATTGCTAT GTATGACTAT GCAGCAAATA ATGAAGATGA GCTCAGTTTC 
TCCAAGGGAC AACTCATTAA TGTTATGAAC AAAGATGATC CTGATTGGTG GCAAGGAGAG 
ATCAACGGGG TGACTGGTCT CTTTCCTTCA AACTACGTTA AGATGACGAC AGACTCAGAT 
CCAAGTCAAC AGTGGTGTGC TGATCTGCAA ACCCTGGACA CAATGCAGCC AATTGAGAGG 
AAAAGACAGG GCTATATTCA TGAGCTGATT CAGACCGAAG AGCGGTACAT GGCTGACCTT 
CAGCTCGTCG TCGAGGTTTT TCAGAAACGC ATGGCAGAGT CAGGCTTTCT CACTGAAGGG 
GAGATGGCCC TGATTTTTGT TAACTGGAAG GAGCTCATCA TGTCCAACAC AAAGCTGCTG 
AAGGCTTTGC GGGTGCGGAA GAAGACCGGG GGCGAGAAGA TGCCGGTGCA GATGATTGGG 
GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCTTACA TCAGGTTCTG CAGCTGCCAG 
CTTAATGGAG CAGCTCTGTT ACAGCAGAAG ACAGATGAAG ACACAGATTT CAAAGAATTT 
TTAAAGAAGC TGGCATCTGA CCCGCGGTGT AAAGGAATGC CCCTCTCCAG CTTCCTGCTG 
AAACCCATGC AGAGGATCAC CCGCTACCCA CTGCTCATCA GAAGTATTCT GGAGAACACC 
CCGGAGAGCC ATGCAGACCA TTCCTCCCTA AAGCTGGCCC TCGAGCGGGC AGAGGAGCTG 
TGCTCTCAAG TGAATGAGGG AGTTCGGGAG AAGGAAAACT CGGACCGACT GGAGTGGATC 
CAGGCGCACG TGCAGTGTGA AGGCCTCGCG GAGCAACTTA TTTTCAACTC TCTCACCAAC 
TGCCTGGGGC CCCGGAAGCT CTTACACAGT GGGAAATTAT ACAAGACCAA GAGCAACAAG 
GAACTGCACG GATTCCTCTT CAATGACTTC CTGCTTCTTA CCTACATGGT CAAGCAGTTT 
GCTGTTTCCT CTGGCTCTGA GAAACTTTTC AGCTCGAAGT CCAATGCTCA ATTCAAAATG 
TATAAAACGC CCATTTTCCT GAATGAAGTC TTGGTGAAAC TGCCCACAGA CCCTTCCAGC 
GATGAGCCTG TCTTCCACAT TTCCCACATT GATCGGGTCT ACACCCTCCG AACAGACAAC 
ATTAATGAGA GGACCGCCTG GGTGCAGAAG ATCAAGGCGG CGTCTGAGCA GTACATCGAC 
ACCGAGAAGA AGAAGCGTGA GAAAGCTTAC CAAGCCCGCT CCCAAAAGAC TTCAGGCATT 
GGGCGCCTGA TGGTGCATGT CATTGAAGCT ACAGAATTAA AAGCCTGCAA ACCAAATGGA 
AAGAGCAACC CATACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGGACCATC 
CAGGACACAC TCAATCCCAA GTGGAATTTT AACTGCCAGT TCTTTATTAA GGATCTCTAC 
CAAGACGTGC TGTGTCTCAC CCTGTTTGAC AGAGACCAGT TTTCACCAGA TGATTTCCTG 
GGTCGTACTG AAATTCCAGT GGCAAAAATT CGAACAGAAC AGGAAAGCAA AGGCCCTATG 
ACCCGCCGAC TGCTGCTGCA TGAGGTCCCC ACCGGGGAGG TCTGGGTCCG TTTTGACCTG 
CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308705.1
CDS	853..3642
Translation	MENGLENRDTTTIFIYSVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452937.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452937.1

ORF Insert Sequence:

ATGGAAAATG GATTGGAGAA CAGAGACACA ACTACCATAT TCATTTATTC AGTTGATGAA 
AAAACCGTAG GAGAACCTGG TTGGCTTTAT GGTAGTTTTC AAGGAAATTT TGGCTGGTTT 
CCATGCAATT ATGTAGAAAA AATGCCATCA AGTGAAAATG AAAAAGCTGT ATCTCCAAAG 
AAGGCCTTAC TTCCTCCTAC AGTTTCTTTA TCTGCTACCT CAACTTCCTC TGAACCACTT 
TCTTCAAATC AACCAGCATC AGTGACTGAT TATCAAAATG TATCTTTTTC AAACCTAACT 
GTAAATACAT CATGGCAGAA AAAATCAGCC TTCACTCGAA CTGTGTCCCC TGGATCTGTA 
TCACCTATTC ATGGACAGGG ACAAGTGGTA GAAAACTTAA AAGCACAGGC CCTTTGTTCC 
TGGACTGCAA AGAAAGATAA CCACTTGAAC TTCTCAAAAC ATGACATTAT TACTGTCTTG 
GAGCAGCAAG AAAATTGGTG GTTTGGGGAG GTGCATGGAG GAAGAGGATG GTTTCCCAAA 
TCTTATGTCA AGATCATTCC TGGGAGTGAA GTAAAACGGG AAGAACCAGA AGCTTTGTAT 
GCAGCTGTAA ATAAGAAACC TACCTCGGCA GCCTATTCAG TTGGAGAAGA ATATATTGCA 
CTTTATCCAT ATTCAAGTGT GGAACCTGGA GATTTGACTT TCACAGAAGG TGAAGAAATA 
TTGGTGACCC AGAAAGATGG AGAGTGGTGG ACAGGAAGTA TTGGAGATAG AAGTGGAATT 
TTTCCATCAA ACTATGTCAA ACCAAAGGAT CAAGAGAGTT TTGGGAGTGC TAGCAAGTCT 
GGAGCATCAA ATAAAAAACC TGAGATTGCT CAGGTAACTT CAGCATATGT TGCTTCTGGT 
TCTGAACAAC TTAGCCTTGC ACCAGGACAG TTAATATTAA TTCTAAAGAA AAATACAAGT 
GGGTGGTGGC AAGGAGAGTT ACAGGCCAGA GGAAAAAAGC GACAGAAAGG ATGGTTTCCT 
GCCAGTCATG TTAAACTTTT GGGTCCAAGT AGTGAAAGAG CCACACCTGC CTTTCATCCT 
GTATGTCAGG TGATTGCTAT GTATGACTAT GCAGCAAATA ATGAAGATGA GCTCAGTTTC 
TCCAAGGGAC AACTCATTAA TGTTATGAAC AAAGATGATC CTGATTGGTG GCAAGGAGAG 
ATCAACGGGG TGACTGGTCT CTTTCCTTCA AACTACGTTA AGATGACGAC AGACTCAGAT 
CCAAGTCAAC AGTGGTGTGC TGATCTGCAA ACCCTGGACA CAATGCAGCC AATTGAGAGG 
AAAAGACAGG GCTATATTCA TGAGCTGATT CAGACCGAAG AGCGGTACAT GGCTGACCTT 
CAGCTCGTCG TCGAGGTTTT TCAGAAACGC ATGGCAGAGT CAGGCTTTCT CACTGAAGGG 
GAGATGGCCC TGATTTTTGT TAACTGGAAG GAGCTCATCA TGTCCAACAC AAAGCTGCTG 
AAGGCTTTGC GGGTGCGGAA GAAGACCGGG GGCGAGAAGA TGCCGGTGCA GATGATTGGG 
GACATCCTGG CCGCTGAGCT GTCCCACATG CAGGCTTACA TCAGGTTCTG CAGCTGCCAG 
CTTAATGGAG CAGCTCTGTT ACAGCAGAAG ACAGATGAAG ACACAGATTT CAAAGAATTT 
TTAAAGAAGC TGGCATCTGA CCCGCGGTGT AAAGGAATGC CCCTCTCCAG CTTCCTGCTG 
AAACCCATGC AGAGGATCAC CCGCTACCCA CTGCTCATCA GAAGTATTCT GGAGAACACC 
CCGGAGAGCC ATGCAGACCA TTCCTCCCTA AAGCTGGCCC TCGAGCGGGC AGAGGAGCTG 
TGCTCTCAAG TGAATGAGGG AGTTCGGGAG AAGGAAAACT CGGACCGACT GGAGTGGATC 
CAGGCGCACG TGCAGTGTGA AGGCCTCGCG GAGCAACTTA TTTTCAACTC TCTCACCAAC 
TGCCTGGGGC CCCGGAAGCT CTTACACAGT GGGAAATTAT ACAAGACCAA GAGCAACAAG 
GAACTGCACG GATTCCTCTT CAATGACTTC CTGCTTCTTA CCTACATGGT CAAGCAGTTT 
GCTGTTTCCT CTGGCTCTGA GAAACTTTTC AGCTCGAAGT CCAATGCTCA ATTCAAAATG 
TATAAAACGC CCATTTTCCT GAATGAAGTC TTGGTGAAAC TGCCCACAGA CCCTTCCAGC 
GATGAGCCTG TCTTCCACAT TTCCCACATT GATCGGGTCT ACACCCTCCG AACAGACAAC 
ATTAATGAGA GGACCGCCTG GGTGCAGAAG ATCAAGGCGG CGTCTGAGCA GTACATCGAC 
ACCGAGAAGA AGAAGCGTGA GAAAGCTTAC CAAGCCCGCT CCCAAAAGAC TTCAGGCATT 
GGGCGCCTGA TGGTGCATGT CATTGAAGCT ACAGAATTAA AAGCCTGCAA ACCAAATGGA 
AAGAGCAACC CATACTGTGA AATCAGCATG GGCTCCCAGA GCTACACCAC CAGGACCATC 
CAGGACACAC TCAATCCCAA GTGGAATTTT AACTGCCAGT TCTTTATTAA GGATCTCTAC 
CAAGACGTGC TGTGTCTCAC CCTGTTTGAC AGAGACCAGT TTTCACCAGA TGATTTCCTG 
GGTCGTACTG AAATTCCAGT GGCAAAAATT CGAACAGAAC AGGAAAGCAA AGGCCCTATG 
ACCCGCCGAC TGCTGCTGCA TGAGGTCCCC ACCGGGGAGG TCTGGGTCCG TTTTGACCTG 
CAGCTTTTTG AGCAAAAAAC TCTCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348183.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335112.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu100853
Clone ID Related Accession (Same CDS sequence)	NM_001348183.1 , NM_001348183.2
Accession Version	NM_001348183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.206162.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335112.1
CDS	259..3756
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348183.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348183.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348183.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335112.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.

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CloneID	OHu100853
Clone ID Related Accession (Same CDS sequence)	NM_001348183.1 , NM_001348183.2
Accession Version	NM_001348183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. On Jun 2, 2019 this sequence version replaced NM_001348183.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.206162.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335112.1
CDS	267..3764
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348183.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348186.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335115.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu100854
Clone ID Related Accession (Same CDS sequence)	NM_001348186.1 , NM_001348186.2
Accession Version	NM_001348186.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.87835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335115.1
CDS	259..3717
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348186.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348186.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348186.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335115.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu100854
Clone ID Related Accession (Same CDS sequence)	NM_001348186.1 , NM_001348186.2
Accession Version	NM_001348186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. On Jun 2, 2019 this sequence version replaced NM_001348186.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.87835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335115.1
CDS	267..3725
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348186.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348186.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348185.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335114.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.

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CloneID	OHu100855
Clone ID Related Accession (Same CDS sequence)	NM_001348185.1 , NM_001348185.2
Accession Version	NM_001348185.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.95328.1, SRR1803611.169759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335114.1
CDS	259..3798
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348185.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348185.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348185.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335114.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.

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CloneID	OHu100855
Clone ID Related Accession (Same CDS sequence)	NM_001348185.1 , NM_001348185.2
Accession Version	NM_001348185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. On Jun 2, 2019 this sequence version replaced NM_001348185.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.95328.1, SRR1803611.169759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335114.1
CDS	267..3806
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348185.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348185.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348184.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335113.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu100857
Clone ID Related Accession (Same CDS sequence)	NM_001348184.1 , NM_001348184.2
Accession Version	NM_001348184.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.280568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335113.1
CDS	368..3823
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348184.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348184.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348184.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335113.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.

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CloneID	OHu100857
Clone ID Related Accession (Same CDS sequence)	NM_001348184.1 , NM_001348184.2
Accession Version	NM_001348184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4. On Jun 2, 2019 this sequence version replaced NM_001348184.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.280568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335113.1
CDS	376..3831
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	NM_001348184.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348184.2

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452936.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308704.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X8, mRNA.

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CloneID	OHu104906
Clone ID Related Accession (Same CDS sequence)	XM_024452936.1
Accession Version	XM_024452936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308704.1
CDS	31..3567
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_024452936.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452936.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_147152.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_671494.2 Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.

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CloneID	OHu10175
Clone ID Related Accession (Same CDS sequence)	NM_147152.2 , NM_147152.3
Accession Version	NM_147152.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA382952.1, AF182199.1 and AF038189.1. On Jul 17, 2008 this sequence version replaced NM_147152.1. Transcript Variant: This variant (2), also known as ITSN2-S1, has an alternate 3' coding region and 3' UTR compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TGAGGAGGCT GCTGCTGGCT 
AGCTCTCGGG GTATCTGCTG TCTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_671494.2
CDS	259..4008
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVRRLLLASSRGICCLS

Target ORF information:

RefSeq Version	NM_147152.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147152.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TGAGGAGGCT GCTGCTGGCT 
AGCTCTCGGG GTATCTGCTG TCTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_147152.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_671494.2 Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10175
Clone ID Related Accession (Same CDS sequence)	NM_147152.2 , NM_147152.3
Accession Version	NM_147152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4, DA382952.1, AF182199.1 and AF038189.1. On Jun 2, 2019 this sequence version replaced NM_147152.2. Transcript Variant: This variant (2), also known as ITSN2-S1, has an alternate 3' coding region and 3' UTR compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TGAGGAGGCT GCTGCTGGCT 
AGCTCTCGGG GTATCTGCTG TCTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_671494.2
CDS	267..4016
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVRRLLLASSRGICCLS

Target ORF information:

RefSeq Version	NM_147152.3
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147152.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TGAGGAGGCT GCTGCTGGCT 
AGCTCTCGGG GTATCTGCTG TCTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348181.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335110.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100852
Clone ID Related Accession (Same CDS sequence)	XM_024452932.1 , NM_001348181.1 , NM_001348181.2
Accession Version	NM_001348181.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4 and AC008073.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.261156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335110.1
CDS	368..5419
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_001348181.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348181.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348181.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335110.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.

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CloneID	OHu100852
Clone ID Related Accession (Same CDS sequence)	XM_024452932.1 , NM_001348181.1 , NM_001348181.2
Accession Version	NM_001348181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4 and AC008073.4. On Jun 2, 2019 this sequence version replaced NM_001348181.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.261156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335110.1
CDS	376..5427
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_001348181.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348181.2

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348182.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335111.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.

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CloneID	OHu100856
Clone ID Related Accession (Same CDS sequence)	NM_001348182.1 , NM_001348182.2
Accession Version	NM_001348182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4 and AC008073.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.188313.1, SRR1803615.244826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335111.1
CDS	259..5232
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_001348182.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348182.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_001348182.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335111.1 Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.

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CloneID	OHu100856
Clone ID Related Accession (Same CDS sequence)	NM_001348182.1 , NM_001348182.2
Accession Version	NM_001348182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4 and AC008073.4. On Jun 2, 2019 this sequence version replaced NM_001348182.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2440478.1, SRR1803616.188313.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335111.1
CDS	267..5240
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_001348182.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348182.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452932.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308700.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OHu100852
Clone ID Related Accession (Same CDS sequence)	XM_024452932.1 , NM_001348181.1 , NM_001348181.2
Accession Version	XM_024452932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308700.1
CDS	411..5462
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452932.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452932.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG 
TCTCTGCTAA GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC 
AACACACAGC AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA 
ATTGAAAGGA AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG 
AAAGCAAAGC AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA 
AAACAAAAGC GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA 
GCTGAGGAGA AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG 
ACAGCTAGTG TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT 
GAGATGAGTT TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT 
GGTTGGCTTT ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA 
AAAATGCCAT CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT 
ACAGTTTCTT TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA 
TCAGTGACTG ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG 
AAAAAATCAG CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG 
GGACAAGTGG TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT 
AACCACTTGA ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG 
TGGTTTGGGG AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT 
CCTGGGAGTG AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA 
CCTACCTCGG CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT 
GTGGAACCTG GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT 
GGAGAGTGGT GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC 
AAACCAAAGG ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA 
CCTGAGATTG CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT 
GCACCAGGAC AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG 
TTACAGGCCA GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT 
TTGGGTCCAA GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT 
ATGTATGACT ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT 
AATGTTATGA ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT 
CTCTTTCCTT CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT 
GCTGATCTGC AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT 
CATGAGCTGA TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTT 
TTTCAGAAAC GCATGGCAGA GTCAGGCTTT CTCACTGAAG GGGAGATGGC CCTGATTTTT 
GTTAACTGGA AGGAGCTCAT CATGTCCAAC ACAAAGCTGC TGAAGGCTTT GCGGGTGCGG 
AAGAAGACCG GGGGCGAGAA GATGCCGGTG CAGATGATTG GGGACATCCT GGCCGCTGAG 
CTGTCCCACA TGCAGGCTTA CATCAGGTTC TGCAGCTGCC AGCTTAATGG AGCAGCTCTG 
TTACAGCAGA AGACAGATGA AGACACAGAT TTCAAAGAAT TTTTAAAGAA GCTGGCATCT 
GACCCGCGGT GTAAAGGAAT GCCCCTCTCC AGCTTCCTGC TGAAACCCAT GCAGAGGATC 
ACCCGCTACC CACTGCTCAT CAGAAGTATT CTGGAGAACA CCCCGGAGAG CCATGCAGAC 
CATTCCTCCC TAAAGCTGGC CCTCGAGCGG GCAGAGGAGC TGTGCTCTCA AGTGAATGAG 
GGAGTTCGGG AGAAGGAAAA CTCGGACCGA CTGGAGTGGA TCCAGGCGCA CGTGCAGTGT 
GAAGGCCTCG CGGAGCAACT TATTTTCAAC TCTCTCACCA ACTGCCTGGG GCCCCGGAAG 
CTCTTACACA GTGGGAAATT ATACAAGACC AAGAGCAACA AGGAACTGCA CGGATTCCTC 
TTCAATGACT TCCTGCTTCT TACCTACATG GTCAAGCAGT TTGCTGTTTC CTCTGGCTCT 
GAGAAACTTT TCAGCTCGAA GTCCAATGCT CAATTCAAAA TGTATAAAAC GCCCATTTTC 
CTGAATGAAG TCTTGGTGAA ACTGCCCACA GACCCTTCCA GCGATGAGCC TGTCTTCCAC 
ATTTCCCACA TTGATCGGGT CTACACCCTC CGAACAGACA ACATTAATGA GAGGACCGCC 
TGGGTGCAGA AGATCAAGGC GGCGTCTGAG CAGTACATCG ACACCGAGAA GAAGAAGCGT 
GAGAAAGCTT ACCAAGCCCG CTCCCAAAAG ACTTCAGGCA TTGGGCGCCT GATGGTGCAT 
GTCATTGAAG CTACAGAATT AAAAGCCTGC AAACCAAATG GAAAGAGCAA CCCATACTGT 
GAAATCAGCA TGGGCTCCCA GAGCTACACC ACCAGGACCA TCCAGGACAC ACTCAATCCC 
AAGTGGAATT TTAACTGCCA GTTCTTTATT AAGGATCTCT ACCAAGACGT GCTGTGTCTC 
ACCCTGTTTG ACAGAGACCA GTTTTCACCA GATGATTTCC TGGGTCGTAC TGAAATTCCA 
GTGGCAAAAA TTCGAACAGA ACAGGAAAGC AAAGGCCCTA TGACCCGCCG ACTGCTGCTG 
CATGAGGTCC CCACCGGGGA GGTCTGGGTC CGTTTTGACC TGCAGCTTTT TGAGCAAAAA 
ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452933.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308701.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu104904
Clone ID Related Accession (Same CDS sequence)	XM_024452933.1
Accession Version	XM_024452933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGG 
TGTGCTGATC TGCAAACCCT GGACACAATG CAGCCAATTG AGAGGAAAAG ACAGGGCTAT 
ATTCATGAGC TGATTCAGAC CGAAGAGCGG TACATGGCTG ACCTTCAGCT CGTCGTCGAG 
GTTTTTCAGA AACGCATGGC AGAGTCAGGC TTTCTCACTG AAGGGGAGAT GGCCCTGATT 
TTTGTTAACT GGAAGGAGCT CATCATGTCC AACACAAAGC TGCTGAAGGC TTTGCGGGTG 
CGGAAGAAGA CCGGGGGCGA GAAGATGCCG GTGCAGATGA TTGGGGACAT CCTGGCCGCT 
GAGCTGTCCC ACATGCAGGC TTACATCAGG TTCTGCAGCT GCCAGCTTAA TGGAGCAGCT 
CTGTTACAGC AGAAGACAGA TGAAGACACA GATTTCAAAG AATTTTTAAA GAAGCTGGCA 
TCTGACCCGC GGTGTAAAGG AATGCCCCTC TCCAGCTTCC TGCTGAAACC CATGCAGAGG 
ATCACCCGCT ACCCACTGCT CATCAGAAGT ATTCTGGAGA ACACCCCGGA GAGCCATGCA 
GACCATTCCT CCCTAAAGCT GGCCCTCGAG CGGGCAGAGG AGCTGTGCTC TCAAGTGAAT 
GAGGGAGTTC GGGAGAAGGA AAACTCGGAC CGACTGGAGT GGATCCAGGC GCACGTGCAG 
TGTGAAGGCC TCGCGGAGCA ACTTATTTTC AACTCTCTCA CCAACTGCCT GGGGCCCCGG 
AAGCTCTTAC ACAGTGGGAA ATTATACAAG ACCAAGAGCA ACAAGGAACT GCACGGATTC 
CTCTTCAATG ACTTCCTGCT TCTTACCTAC ATGGTCAAGC AGTTTGCTGT TTCCTCTGGC 
TCTGAGAAAC TTTTCAGCTC GAAGTCCAAT GCTCAATTCA AAATGTATAA AACGCCCATT 
TTCCTGAATG AAGTCTTGGT GAAACTGCCC ACAGACCCTT CCAGCGATGA GCCTGTCTTC 
CACATTTCCC ACATTGATCG GGTCTACACC CTCCGAACAG ACAACATTAA TGAGAGGACC 
GCCTGGGTGC AGAAGATCAA GGCGGCGTCT GAGCAGTACA TCGACACCGA GAAGAAGAAG 
CGTGAGAAAG CTTACCAAGC CCGCTCCCAA AAGACTTCAG GCATTGGGCG CCTGATGGTG 
CATGTCATTG AAGCTACAGA ATTAAAAGCC TGCAAACCAA ATGGAAAGAG CAACCCATAC 
TGTGAAATCA GCATGGGCTC CCAGAGCTAC ACCACCAGGA CCATCCAGGA CACACTCAAT 
CCCAAGTGGA ATTTTAACTG CCAGTTCTTT ATTAAGGATC TCTACCAAGA CGTGCTGTGT 
CTCACCCTGT TTGACAGAGA CCAGTTTTCA CCAGATGATT TCCTGGGTCG TACTGAAATT 
CCAGTGGCAA AAATTCGAAC AGAACAGGAA AGCAAAGGCC CTATGACCCG CCGACTGCTG 
CTGCATGAGG TCCCCACCGG GGAGGTCTGG GTCCGTTTTG ACCTGCAGCT TTTTGAGCAA 
AAAACTCTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308701.1
CDS	264..5318
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452933.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452933.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
GAGACAGCTA GTGTTTTGGT GAATTATAGA GCATTATACC CCTTTGAAGC AAGGAACCAT 
GATGAGATGA GTTTTAATTC TGGAGATATA ATTCAGGTTG ATGAAAAAAC CGTAGGAGAA 
CCTGGTTGGC TTTATGGTAG TTTTCAAGGA AATTTTGGCT GGTTTCCATG CAATTATGTA 
GAAAAAATGC CATCAAGTGA AAATGAAAAA GCTGTATCTC CAAAGAAGGC CTTACTTCCT 
CCTACAGTTT CTTTATCTGC TACCTCAACT TCCTCTGAAC CACTTTCTTC AAATCAACCA 
GCATCAGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAACTGTAAA TACATCATGG 
CAGAAAAAAT CAGCCTTCAC TCGAACTGTG TCCCCTGGAT CTGTATCACC TATTCATGGA 
CAGGGACAAG TGGTAGAAAA CTTAAAAGCA CAGGCCCTTT GTTCCTGGAC TGCAAAGAAA 
GATAACCACT TGAACTTCTC AAAACATGAC ATTATTACTG TCTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTGCA TGGAGGAAGA GGATGGTTTC CCAAATCTTA TGTCAAGATC 
ATTCCTGGGA GTGAAGTAAA ACGGGAAGAA CCAGAAGCTT TGTATGCAGC TGTAAATAAG 
AAACCTACCT CGGCAGCCTA TTCAGTTGGA GAAGAATATA TTGCACTTTA TCCATATTCA 
AGTGTGGAAC CTGGAGATTT GACTTTCACA GAAGGTGAAG AAATATTGGT GACCCAGAAA 
GATGGAGAGT GGTGGACAGG AAGTATTGGA GATAGAAGTG GAATTTTTCC ATCAAACTAT 
GTCAAACCAA AGGATCAAGA GAGTTTTGGG AGTGCTAGCA AGTCTGGAGC ATCAAATAAA 
AAACCTGAGA TTGCTCAGGT AACTTCAGCA TATGTTGCTT CTGGTTCTGA ACAACTTAGC 
CTTGCACCAG GACAGTTAAT ATTAATTCTA AAGAAAAATA CAAGTGGGTG GTGGCAAGGA 
GAGTTACAGG CCAGAGGAAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG TCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAGCCACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCTATGTATG ACTATGCAGC AAATAATGAA GATGAGCTCA GTTTCTCCAA GGGACAACTC 
ATTAATGTTA TGAACAAAGA TGATCCTGAT TGGTGGCAAG GAGAGATCAA CGGGGTGACT 
GGTCTCTTTC CTTCAAACTA CGTTAAGATG ACGACAGACT CAGATCCAAG TCAACAGTGG 
TGTGCTGATC TGCAAACCCT GGACACAATG CAGCCAATTG AGAGGAAAAG ACAGGGCTAT 
ATTCATGAGC TGATTCAGAC CGAAGAGCGG TACATGGCTG ACCTTCAGCT CGTCGTCGAG 
GTTTTTCAGA AACGCATGGC AGAGTCAGGC TTTCTCACTG AAGGGGAGAT GGCCCTGATT 
TTTGTTAACT GGAAGGAGCT CATCATGTCC AACACAAAGC TGCTGAAGGC TTTGCGGGTG 
CGGAAGAAGA CCGGGGGCGA GAAGATGCCG GTGCAGATGA TTGGGGACAT CCTGGCCGCT 
GAGCTGTCCC ACATGCAGGC TTACATCAGG TTCTGCAGCT GCCAGCTTAA TGGAGCAGCT 
CTGTTACAGC AGAAGACAGA TGAAGACACA GATTTCAAAG AATTTTTAAA GAAGCTGGCA 
TCTGACCCGC GGTGTAAAGG AATGCCCCTC TCCAGCTTCC TGCTGAAACC CATGCAGAGG 
ATCACCCGCT ACCCACTGCT CATCAGAAGT ATTCTGGAGA ACACCCCGGA GAGCCATGCA 
GACCATTCCT CCCTAAAGCT GGCCCTCGAG CGGGCAGAGG AGCTGTGCTC TCAAGTGAAT 
GAGGGAGTTC GGGAGAAGGA AAACTCGGAC CGACTGGAGT GGATCCAGGC GCACGTGCAG 
TGTGAAGGCC TCGCGGAGCA ACTTATTTTC AACTCTCTCA CCAACTGCCT GGGGCCCCGG 
AAGCTCTTAC ACAGTGGGAA ATTATACAAG ACCAAGAGCA ACAAGGAACT GCACGGATTC 
CTCTTCAATG ACTTCCTGCT TCTTACCTAC ATGGTCAAGC AGTTTGCTGT TTCCTCTGGC 
TCTGAGAAAC TTTTCAGCTC GAAGTCCAAT GCTCAATTCA AAATGTATAA AACGCCCATT 
TTCCTGAATG AAGTCTTGGT GAAACTGCCC ACAGACCCTT CCAGCGATGA GCCTGTCTTC 
CACATTTCCC ACATTGATCG GGTCTACACC CTCCGAACAG ACAACATTAA TGAGAGGACC 
GCCTGGGTGC AGAAGATCAA GGCGGCGTCT GAGCAGTACA TCGACACCGA GAAGAAGAAG 
CGTGAGAAAG CTTACCAAGC CCGCTCCCAA AAGACTTCAG GCATTGGGCG CCTGATGGTG 
CATGTCATTG AAGCTACAGA ATTAAAAGCC TGCAAACCAA ATGGAAAGAG CAACCCATAC 
TGTGAAATCA GCATGGGCTC CCAGAGCTAC ACCACCAGGA CCATCCAGGA CACACTCAAT 
CCCAAGTGGA ATTTTAACTG CCAGTTCTTT ATTAAGGATC TCTACCAAGA CGTGCTGTGT 
CTCACCCTGT TTGACAGAGA CCAGTTTTCA CCAGATGATT TCCTGGGTCG TACTGAAATT 
CCAGTGGCAA AAATTCGAAC AGAACAGGAA AGCAAAGGCC CTATGACCCG CCGACTGCTG 
CTGCATGAGG TCCCCACCGG GGAGGTCTGG GTCCGTTTTG ACCTGCAGCT TTTTGAGCAA 
AAAACTCTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452935.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308703.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu104905
Clone ID Related Accession (Same CDS sequence)	XM_024452935.1
Accession Version	XM_024452935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGGTGTG CTGATCTGCA AACCCTGGAC 
ACAATGCAGC CAATTGAGAG GAAAAGACAG GGCTATATTC ATGAGCTGAT TCAGACCGAA 
GAGCGGTACA TGGCTGACCT TCAGCTCGTC GTCGAGGTTT TTCAGAAACG CATGGCAGAG 
TCAGGCTTTC TCACTGAAGG GGAGATGGCC CTGATTTTTG TTAACTGGAA GGAGCTCATC 
ATGTCCAACA CAAAGCTGCT GAAGGCTTTG CGGGTGCGGA AGAAGACCGG GGGCGAGAAG 
ATGCCGGTGC AGATGATTGG GGACATCCTG GCCGCTGAGC TGTCCCACAT GCAGGCTTAC 
ATCAGGTTCT GCAGCTGCCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA 
GACACAGATT TCAAAGAATT TTTAAAGAAG CTGGCATCTG ACCCGCGGTG TAAAGGAATG 
CCCCTCTCCA GCTTCCTGCT GAAACCCATG CAGAGGATCA CCCGCTACCC ACTGCTCATC 
AGAAGTATTC TGGAGAACAC CCCGGAGAGC CATGCAGACC ATTCCTCCCT AAAGCTGGCC 
CTCGAGCGGG CAGAGGAGCT GTGCTCTCAA GTGAATGAGG GAGTTCGGGA GAAGGAAAAC 
TCGGACCGAC TGGAGTGGAT CCAGGCGCAC GTGCAGTGTG AAGGCCTCGC GGAGCAACTT 
ATTTTCAACT CTCTCACCAA CTGCCTGGGG CCCCGGAAGC TCTTACACAG TGGGAAATTA 
TACAAGACCA AGAGCAACAA GGAACTGCAC GGATTCCTCT TCAATGACTT CCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCTGGCTCTG AGAAACTTTT CAGCTCGAAG 
TCCAATGCTC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCCCACAT TGATCGGGTC 
TACACCCTCC GAACAGACAA CATTAATGAG AGGACCGCCT GGGTGCAGAA GATCAAGGCG 
GCGTCTGAGC AGTACATCGA CACCGAGAAG AAGAAGCGTG AGAAAGCTTA CCAAGCCCGC 
TCCCAAAAGA CTTCAGGCAT TGGGCGCCTG ATGGTGCATG TCATTGAAGC TACAGAATTA 
AAAGCCTGCA AACCAAATGG AAAGAGCAAC CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTACACCA CCAGGACCAT CCAGGACACA CTCAATCCCA AGTGGAATTT TAACTGCCAG 
TTCTTTATTA AGGATCTCTA CCAAGACGTG CTGTGTCTCA CCCTGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAATTCCAG TGGCAAAAAT TCGAACAGAA 
CAGGAAAGCA AAGGCCCTAT GACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GTTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308703.1
CDS	378..5348
Translation	MWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452935.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452935.1

ORF Insert Sequence:

ATGTGGGCTA TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG 
CCGGCCCCTG TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG 
GATCAGCAAG AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG 
TTGCCTGTGG TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT 
GCTCGTTTTG GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT 
GCACCTATAA CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG 
CCCACTCCCC TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC 
ATTCAGCCTT TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT 
CTGATGATGG GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA 
GGATCTAGTA GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG 
ACCTCAGAGT GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT 
CTTGACAAAA GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG 
TCAAATCTTT CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT 
GGACAGCTAA AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT 
GGACAGCCAT TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA 
AAGCAAATTG ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG 
CCTCAGAAGA AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG 
AACATGGAGC TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA 
CGTAAAGCCC AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA 
GAATGGAAGA AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA 
CAACGAGAGG AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT 
GAACGACAAC GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG 
AATAGAGAAC AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG 
TTGGAAGCAC TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC 
AAAAAGCAAA CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT 
ATGGAAATCA AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG 
GTACCTGAGA AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT 
GATTCAGGGG TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA 
CTTAAAGAAC AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT 
TCTTTTAACA ATCAACTAAA GGAACTGAGA GAAACCTACA ACACACAGCA GTTAGCCCTT 
GAACAGCTTT ATAAGATCAA ACGTGACAAG TTGAAGGAAA TTGAAAGGAA AAGATTAGAA 
CTAATGCAGA AAAAGAAACT AGAAGATGAG GCTGCAAGGA AAGCAAAGCA AGGAAAAGAA 
AACTTATGGA AAGAAAATCT TAGAAAGGAG GAAGAAGAAA AACAAAAGCG ACTCCAGGAA 
GAAAAAACAC AAGAAAAAAT TCAAGAAGAG GAACGGAAAG CTGAGGAGAA ACAACGTAAG 
GATAAGGATA CTTTGAAAGC TGAGGAGAAA AAACGTGAGA CAGCTAGTGT TTTGGTGAAT 
TATAGAGCAT TATACCCCTT TGAAGCAAGG AACCATGATG AGATGAGTTT TAATTCTGGA 
GATATAATTC AGGTTGATGA AAAAACCGTA GGAGAACCTG GTTGGCTTTA TGGTAGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAATGCCATC AAGTGAAAAT 
GAAAAAGCTG TATCTCCAAA GAAGGCCTTA CTTCCTCCTA CAGTTTCTTT ATCTGCTACC 
TCAACTTCCT CTGAACCACT TTCTTCAAAT CAACCAGCAT CAGTGACTGA TTATCAAAAT 
GTATCTTTTT CAAACCTAAC TGTAAATACA TCATGGCAGA AAAAATCAGC CTTCACTCGA 
ACTGTGTCCC CTGGATCTGT ATCACCTATT CATGGACAGG GACAAGTGGT AGAAAACTTA 
AAAGCACAGG CCCTTTGTTC CTGGACTGCA AAGAAAGATA ACCACTTGAA CTTCTCAAAA 
CATGACATTA TTACTGTCTT GGAGCAGCAA GAAAATTGGT GGTTTGGGGA GGTGCATGGA 
GGAAGAGGAT GGTTTCCCAA ATCTTATGTC AAGATCATTC CTGGGAGTGA AGTAAAACGG 
GAAGAACCAG AAGCTTTGTA TGCAGCTGTA AATAAGAAAC CTACCTCGGC AGCCTATTCA 
GTTGGAGAAG AATATATTGC ACTTTATCCA TATTCAAGTG TGGAACCTGG AGATTTGACT 
TTCACAGAAG GTGAAGAAAT ATTGGTGACC CAGAAAGATG GAGAGTGGTG GACAGGAAGT 
ATTGGAGATA GAAGTGGAAT TTTTCCATCA AACTATGTCA AACCAAAGGA TCAAGAGAGT 
TTTGGGAGTG CTAGCAAGTC TGGAGCATCA AATAAAAAAC CTGAGATTGC TCAGGTAACT 
TCAGCATATG TTGCTTCTGG TTCTGAACAA CTTAGCCTTG CACCAGGACA GTTAATATTA 
ATTCTAAAGA AAAATACAAG TGGGTGGTGG CAAGGAGAGT TACAGGCCAG AGGAAAAAAG 
CGACAGAAAG GATGGTTTCC TGCCAGTCAT GTTAAACTTT TGGGTCCAAG TAGTGAAAGA 
GCCACACCTG CCTTTCATCC TGTATGTCAG GTGATTGCTA TGTATGACTA TGCAGCAAAT 
AATGAAGATG AGCTCAGTTT CTCCAAGGGA CAACTCATTA ATGTTATGAA CAAAGATGAT 
CCTGATTGGT GGCAAGGAGA GATCAACGGG GTGACTGGTC TCTTTCCTTC AAACTACGTT 
AAGATGACGA CAGACTCAGA TCCAAGTCAA CAGTGGTGTG CTGATCTGCA AACCCTGGAC 
ACAATGCAGC CAATTGAGAG GAAAAGACAG GGCTATATTC ATGAGCTGAT TCAGACCGAA 
GAGCGGTACA TGGCTGACCT TCAGCTCGTC GTCGAGGTTT TTCAGAAACG CATGGCAGAG 
TCAGGCTTTC TCACTGAAGG GGAGATGGCC CTGATTTTTG TTAACTGGAA GGAGCTCATC 
ATGTCCAACA CAAAGCTGCT GAAGGCTTTG CGGGTGCGGA AGAAGACCGG GGGCGAGAAG 
ATGCCGGTGC AGATGATTGG GGACATCCTG GCCGCTGAGC TGTCCCACAT GCAGGCTTAC 
ATCAGGTTCT GCAGCTGCCA GCTTAATGGA GCAGCTCTGT TACAGCAGAA GACAGATGAA 
GACACAGATT TCAAAGAATT TTTAAAGAAG CTGGCATCTG ACCCGCGGTG TAAAGGAATG 
CCCCTCTCCA GCTTCCTGCT GAAACCCATG CAGAGGATCA CCCGCTACCC ACTGCTCATC 
AGAAGTATTC TGGAGAACAC CCCGGAGAGC CATGCAGACC ATTCCTCCCT AAAGCTGGCC 
CTCGAGCGGG CAGAGGAGCT GTGCTCTCAA GTGAATGAGG GAGTTCGGGA GAAGGAAAAC 
TCGGACCGAC TGGAGTGGAT CCAGGCGCAC GTGCAGTGTG AAGGCCTCGC GGAGCAACTT 
ATTTTCAACT CTCTCACCAA CTGCCTGGGG CCCCGGAAGC TCTTACACAG TGGGAAATTA 
TACAAGACCA AGAGCAACAA GGAACTGCAC GGATTCCTCT TCAATGACTT CCTGCTTCTT 
ACCTACATGG TCAAGCAGTT TGCTGTTTCC TCTGGCTCTG AGAAACTTTT CAGCTCGAAG 
TCCAATGCTC AATTCAAAAT GTATAAAACG CCCATTTTCC TGAATGAAGT CTTGGTGAAA 
CTGCCCACAG ACCCTTCCAG CGATGAGCCT GTCTTCCACA TTTCCCACAT TGATCGGGTC 
TACACCCTCC GAACAGACAA CATTAATGAG AGGACCGCCT GGGTGCAGAA GATCAAGGCG 
GCGTCTGAGC AGTACATCGA CACCGAGAAG AAGAAGCGTG AGAAAGCTTA CCAAGCCCGC 
TCCCAAAAGA CTTCAGGCAT TGGGCGCCTG ATGGTGCATG TCATTGAAGC TACAGAATTA 
AAAGCCTGCA AACCAAATGG AAAGAGCAAC CCATACTGTG AAATCAGCAT GGGCTCCCAG 
AGCTACACCA CCAGGACCAT CCAGGACACA CTCAATCCCA AGTGGAATTT TAACTGCCAG 
TTCTTTATTA AGGATCTCTA CCAAGACGTG CTGTGTCTCA CCCTGTTTGA CAGAGACCAG 
TTTTCACCAG ATGATTTCCT GGGTCGTACT GAAATTCCAG TGGCAAAAAT TCGAACAGAA 
CAGGAAAGCA AAGGCCCTAT GACCCGCCGA CTGCTGCTGC ATGAGGTCCC CACCGGGGAG 
GTCTGGGTCC GTTTTGACCT GCAGCTTTTT GAGCAAAAAA CTCTCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_019595.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_062541.3 Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.

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CloneID	OHu10176
Clone ID Related Accession (Same CDS sequence)	NM_019595.3 , XM_024452934.1 , NM_019595.4
Accession Version	NM_019595.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA382952.1, AF182199.1, BC146779.1 and AF182198.1. On Jul 17, 2008 this sequence version replaced NM_019595.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. This results in a shorter protein (isoform 3) that lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB033082.1, BC146779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062541.3
CDS	259..5271
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_019595.3
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019595.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_019595.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_062541.3 Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.

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CloneID	OHu10176
Clone ID Related Accession (Same CDS sequence)	NM_019595.3 , XM_024452934.1 , NM_019595.4
Accession Version	NM_019595.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009228.4, DA382952.1, AF182199.1, BC146779.1, AF182198.1 and AC008073.4. On Jun 2, 2019 this sequence version replaced NM_019595.3. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. This results in a shorter protein (isoform 3) that lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146779.1, AB033082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062541.3
CDS	267..5279
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_019595.4
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019595.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( NM_006277.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006268.2 Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.

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CloneID	OHu18937
Clone ID Related Accession (Same CDS sequence)	XM_024452930.1 , XM_024452931.1 , NM_006277.2 , NM_006277.3
Accession Version	NM_006277.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	intersectin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA382952.1, AF182198.1 and BC146779.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2008 this sequence version replaced NM_006277.1. Transcript Variant: This variant (1), also known as ITSN2-L, encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006268.2
CDS	259..5352
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_006277.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006277.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452934.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308702.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X5, mRNA.

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CloneID	OHu10176
Clone ID Related Accession (Same CDS sequence)	NM_019595.3 , XM_024452934.1 , NM_019595.4
Accession Version	XM_024452934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308702.1
CDS	463..5475
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452934.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452934.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGGAACTGA GAGAAACCTA CAACACACAG CAGTTAGCCC TTGAACAGCT TTATAAGATC 
AAACGTGACA AGTTGAAGGA AATTGAAAGG AAAAGATTAG AACTAATGCA GAAAAAGAAA 
CTAGAAGATG AGGCTGCAAG GAAAGCAAAG CAAGGAAAAG AAAACTTATG GAAAGAAAAT 
CTTAGAAAGG AGGAAGAAGA AAAACAAAAG CGACTCCAGG AAGAAAAAAC ACAAGAAAAA 
ATTCAAGAAG AGGAACGGAA AGCTGAGGAG AAACAACGTA AGGATAAGGA TACTTTGAAA 
GCTGAGGAGA AAAAACGTGA GACAGCTAGT GTTTTGGTGA ATTATAGAGC ATTATACCCC 
TTTGAAGCAA GGAACCATGA TGAGATGAGT TTTAATTCTG GAGATATAAT TCAGGTTGAT 
GAAAAAACCG TAGGAGAACC TGGTTGGCTT TATGGTAGTT TTCAAGGAAA TTTTGGCTGG 
TTTCCATGCA ATTATGTAGA AAAAATGCCA TCAAGTGAAA ATGAAAAAGC TGTATCTCCA 
AAGAAGGCCT TACTTCCTCC TACAGTTTCT TTATCTGCTA CCTCAACTTC CTCTGAACCA 
CTTTCTTCAA ATCAACCAGC ATCAGTGACT GATTATCAAA ATGTATCTTT TTCAAACCTA 
ACTGTAAATA CATCATGGCA GAAAAAATCA GCCTTCACTC GAACTGTGTC CCCTGGATCT 
GTATCACCTA TTCATGGACA GGGACAAGTG GTAGAAAACT TAAAAGCACA GGCCCTTTGT 
TCCTGGACTG CAAAGAAAGA TAACCACTTG AACTTCTCAA AACATGACAT TATTACTGTC 
TTGGAGCAGC AAGAAAATTG GTGGTTTGGG GAGGTGCATG GAGGAAGAGG ATGGTTTCCC 
AAATCTTATG TCAAGATCAT TCCTGGGAGT GAAGTAAAAC GGGAAGAACC AGAAGCTTTG 
TATGCAGCTG TAAATAAGAA ACCTACCTCG GCAGCCTATT CAGTTGGAGA AGAATATATT 
GCACTTTATC CATATTCAAG TGTGGAACCT GGAGATTTGA CTTTCACAGA AGGTGAAGAA 
ATATTGGTGA CCCAGAAAGA TGGAGAGTGG TGGACAGGAA GTATTGGAGA TAGAAGTGGA 
ATTTTTCCAT CAAACTATGT CAAACCAAAG GATCAAGAGA GTTTTGGGAG TGCTAGCAAG 
TCTGGAGCAT CAAATAAAAA ACCTGAGATT GCTCAGGTAA CTTCAGCATA TGTTGCTTCT 
GGTTCTGAAC AACTTAGCCT TGCACCAGGA CAGTTAATAT TAATTCTAAA GAAAAATACA 
AGTGGGTGGT GGCAAGGAGA GTTACAGGCC AGAGGAAAAA AGCGACAGAA AGGATGGTTT 
CCTGCCAGTC ATGTTAAACT TTTGGGTCCA AGTAGTGAAA GAGCCACACC TGCCTTTCAT 
CCTGTATGTC AGGTGATTGC TATGTATGAC TATGCAGCAA ATAATGAAGA TGAGCTCAGT 
TTCTCCAAGG GACAACTCAT TAATGTTATG AACAAAGATG ATCCTGATTG GTGGCAAGGA 
GAGATCAACG GGGTGACTGG TCTCTTTCCT TCAAACTACG TTAAGATGAC GACAGACTCA 
GATCCAAGTC AACAGTGGTG TGCTGATCTG CAAACCCTGG ACACAATGCA GCCAATTGAG 
AGGAAAAGAC AGGGCTATAT TCATGAGCTG ATTCAGACCG AAGAGCGGTA CATGGCTGAC 
CTTCAGCTCG TCGTCGAGGT TTTTCAGAAA CGCATGGCAG AGTCAGGCTT TCTCACTGAA 
GGGGAGATGG CCCTGATTTT TGTTAACTGG AAGGAGCTCA TCATGTCCAA CACAAAGCTG 
CTGAAGGCTT TGCGGGTGCG GAAGAAGACC GGGGGCGAGA AGATGCCGGT GCAGATGATT 
GGGGACATCC TGGCCGCTGA GCTGTCCCAC ATGCAGGCTT ACATCAGGTT CTGCAGCTGC 
CAGCTTAATG GAGCAGCTCT GTTACAGCAG AAGACAGATG AAGACACAGA TTTCAAAGAA 
TTTTTAAAGA AGCTGGCATC TGACCCGCGG TGTAAAGGAA TGCCCCTCTC CAGCTTCCTG 
CTGAAACCCA TGCAGAGGAT CACCCGCTAC CCACTGCTCA TCAGAAGTAT TCTGGAGAAC 
ACCCCGGAGA GCCATGCAGA CCATTCCTCC CTAAAGCTGG CCCTCGAGCG GGCAGAGGAG 
CTGTGCTCTC AAGTGAATGA GGGAGTTCGG GAGAAGGAAA ACTCGGACCG ACTGGAGTGG 
ATCCAGGCGC ACGTGCAGTG TGAAGGCCTC GCGGAGCAAC TTATTTTCAA CTCTCTCACC 
AACTGCCTGG GGCCCCGGAA GCTCTTACAC AGTGGGAAAT TATACAAGAC CAAGAGCAAC 
AAGGAACTGC ACGGATTCCT CTTCAATGAC TTCCTGCTTC TTACCTACAT GGTCAAGCAG 
TTTGCTGTTT CCTCTGGCTC TGAGAAACTT TTCAGCTCGA AGTCCAATGC TCAATTCAAA 
ATGTATAAAA CGCCCATTTT CCTGAATGAA GTCTTGGTGA AACTGCCCAC AGACCCTTCC 
AGCGATGAGC CTGTCTTCCA CATTTCCCAC ATTGATCGGG TCTACACCCT CCGAACAGAC 
AACATTAATG AGAGGACCGC CTGGGTGCAG AAGATCAAGG CGGCGTCTGA GCAGTACATC 
GACACCGAGA AGAAGAAGCG TGAGAAAGCT TACCAAGCCC GCTCCCAAAA GACTTCAGGC 
ATTGGGCGCC TGATGGTGCA TGTCATTGAA GCTACAGAAT TAAAAGCCTG CAAACCAAAT 
GGAAAGAGCA ACCCATACTG TGAAATCAGC ATGGGCTCCC AGAGCTACAC CACCAGGACC 
ATCCAGGACA CACTCAATCC CAAGTGGAAT TTTAACTGCC AGTTCTTTAT TAAGGATCTC 
TACCAAGACG TGCTGTGTCT CACCCTGTTT GACAGAGACC AGTTTTCACC AGATGATTTC 
CTGGGTCGTA CTGAAATTCC AGTGGCAAAA ATTCGAACAG AACAGGAAAG CAAAGGCCCT 
ATGACCCGCC GACTGCTGCT GCATGAGGTC CCCACCGGGG AGGTCTGGGT CCGTTTTGAC 
CTGCAGCTTT TTGAGCAAAA AACTCTCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452930.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308698.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X1, mRNA.

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CloneID	OHu18937
Clone ID Related Accession (Same CDS sequence)	XM_024452930.1 , XM_024452931.1 , NM_006277.2 , NM_006277.3
Accession Version	XM_024452930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308698.1
CDS	143..5236
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452930.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452930.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_024452931.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308699.1 Homo sapiens intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OHu18937
Clone ID Related Accession (Same CDS sequence)	XM_024452930.1 , XM_024452931.1 , NM_006277.2 , NM_006277.3
Accession Version	XM_024452931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308699.1
CDS	513..5606
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_024452931.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452931.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCTATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGTA CTAAGCATGA CAGGCAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCACGTAA TTTTTTCCTA CAATCAGGTC TGCCGGCCCC TGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTAAACAAG GATGGGAAGA TGGATCAGCA AGAGTTCTCC 
ATAGCTATGA AACTCATCAA ACTGAAGCTT CAAGGCCAAC AGTTGCCTGT GGTTCTCCCT 
CCTATTATGA AGCAACCCCC TATGTTTTCT CCATTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGCCA TTGCCTCCAG CTGCACCTAT AACATCATTG 
TCTTCTGCGA CTTCAGGGAC CAACCTTCCT CCCTTAATGA TGCCCACTCC CCTAGTGCCT 
TCTGTTAGCA CATCATCATT ACCAAATGGA ACCGCCAGTC TCATTCAGCC TTTACCCATT 
CCTTATTCTT CTTCAACATT GCCTCATGGG TCATCTTATA GTCTGATGAT GGGAGGATTT 
GGAGGTGCTA GTATACAGAA AGCGCAGTCT CTGATTGATT TAGGATCTAG TAGCTCAACT 
TCCTCGACTG CTTCACTCTC AGGGAACTCA CCCAAGACTG GGACCTCAGA GTGGGCAGTT 
CCTCAGCCTA CAAGATTAAA ATATCGGCAA AAATTTAATA CTCTTGACAA AAGTATGAGT 
GGATATCTCT CAGGTTTTCA AGCTAGAAAT GCCCTTCTTC AGTCAAATCT TTCTCAAACT 
CAGCTGGCTA CTATTTGGAC TCTGGCTGAC GTTGATGGTG ATGGACAGCT AAAAGCAGAA 
GAGTTTATTC TTGCAATGCA CCTTACTGAC ATGGCCAAAG CTGGACAGCC ATTACCACTG 
ACTTTACCTC CTGAGCTTGT TCCTCCATCT TTCAGAGGAG GAAAGCAAAT TGATTCCATT 
AATGGAACTC TGCCTTCATA TCAGAAAATG CAAGAAGAGG AGCCTCAGAA GAAATTACCA 
GTTACTTTTG AGGACAAACG GAAAGCCAAC TATGAGCGAG GGAACATGGA GCTGGAAAAG 
CGACGCCAAG CCTTGATGGA GCAGCAACAA AGGGAGGCAG AACGTAAAGC CCAGAAAGAA 
AAGGAAGAGT GGGAACGAAA ACAGAGAGAA TTACAAGAAC AAGAATGGAA GAAACAACTT 
GAATTAGAAA AACGCTTAGA GAAGCAACGG GAATTGGAGA GACAACGAGA GGAAGAAAGG 
AGAAAAGACA TAGAAAGACG AGAGGCAGCA AAACAGGAAC TTGAACGACA ACGTCGCTTA 
GAATGGGAGA GAATTCGGCG ACAGGAGCTT CTCAATCAAA AGAATAGAGA ACAAGAAGAA 
ATTGTCAGGT TAAACTCTAA AAAGAAGAAT CTTCATCTTG AGTTGGAAGC ACTGAATGGC 
AAACATCAGC AGATCTCAGG CAGACTTCAG GATGTCCGAC TCAAAAAGCA AACTCAAAAG 
ACTGAGCTGG AAGTTCTGGA TAAGCAGTGT GACTTGGAAA TTATGGAAAT CAAGCAACTT 
CAACAGGAAC TTCAGGAATA TCAGAATAAG CTTATCTATC TGGTACCTGA GAAGCAATTA 
TTAAATGAAA GAATTAAAAA CATGCAGTTC AGTAACACAC CTGATTCAGG GGTCAGTTTA 
CTTCATAAAA AATCATTAGA AAAGGAAGAA TTATGCCAAA GACTTAAAGA ACAGTTAGAT 
GCTCTTGAAA AAGAAACTGC ATCTAAGCTG TCAGAAATGG ATTCTTTTAA CAATCAACTA 
AAGTGTGGGA ATATGGATGA CTCTGTTCTT CAGTGCCTTT TGTCTCTGCT AAGCTGTCTC 
AACAACCTCT TCCTCTTACT TAAGGAACTG AGAGAAACCT ACAACACACA GCAGTTAGCC 
CTTGAACAGC TTTATAAGAT CAAACGTGAC AAGTTGAAGG AAATTGAAAG GAAAAGATTA 
GAACTAATGC AGAAAAAGAA ACTAGAAGAT GAGGCTGCAA GGAAAGCAAA GCAAGGAAAA 
GAAAACTTAT GGAAAGAAAA TCTTAGAAAG GAGGAAGAAG AAAAACAAAA GCGACTCCAG 
GAAGAAAAAA CACAAGAAAA AATTCAAGAA GAGGAACGGA AAGCTGAGGA GAAACAACGT 
AAGGATAAGG ATACTTTGAA AGCTGAGGAG AAAAAACGTG AGACAGCTAG TGTTTTGGTG 
AATTATAGAG CATTATACCC CTTTGAAGCA AGGAACCATG ATGAGATGAG TTTTAATTCT 
GGAGATATAA TTCAGGTTGA TGAAAAAACC GTAGGAGAAC CTGGTTGGCT TTATGGTAGT 
TTTCAAGGAA ATTTTGGCTG GTTTCCATGC AATTATGTAG AAAAAATGCC ATCAAGTGAA 
AATGAAAAAG CTGTATCTCC AAAGAAGGCC TTACTTCCTC CTACAGTTTC TTTATCTGCT 
ACCTCAACTT CCTCTGAACC ACTTTCTTCA AATCAACCAG CATCAGTGAC TGATTATCAA 
AATGTATCTT TTTCAAACCT AACTGTAAAT ACATCATGGC AGAAAAAATC AGCCTTCACT 
CGAACTGTGT CCCCTGGATC TGTATCACCT ATTCATGGAC AGGGACAAGT GGTAGAAAAC 
TTAAAAGCAC AGGCCCTTTG TTCCTGGACT GCAAAGAAAG ATAACCACTT GAACTTCTCA 
AAACATGACA TTATTACTGT CTTGGAGCAG CAAGAAAATT GGTGGTTTGG GGAGGTGCAT 
GGAGGAAGAG GATGGTTTCC CAAATCTTAT GTCAAGATCA TTCCTGGGAG TGAAGTAAAA 
CGGGAAGAAC CAGAAGCTTT GTATGCAGCT GTAAATAAGA AACCTACCTC GGCAGCCTAT 
TCAGTTGGAG AAGAATATAT TGCACTTTAT CCATATTCAA GTGTGGAACC TGGAGATTTG 
ACTTTCACAG AAGGTGAAGA AATATTGGTG ACCCAGAAAG ATGGAGAGTG GTGGACAGGA 
AGTATTGGAG ATAGAAGTGG AATTTTTCCA TCAAACTATG TCAAACCAAA GGATCAAGAG 
AGTTTTGGGA GTGCTAGCAA GTCTGGAGCA TCAAATAAAA AACCTGAGAT TGCTCAGGTA 
ACTTCAGCAT ATGTTGCTTC TGGTTCTGAA CAACTTAGCC TTGCACCAGG ACAGTTAATA 
TTAATTCTAA AGAAAAATAC AAGTGGGTGG TGGCAAGGAG AGTTACAGGC CAGAGGAAAA 
AAGCGACAGA AAGGATGGTT TCCTGCCAGT CATGTTAAAC TTTTGGGTCC AAGTAGTGAA 
AGAGCCACAC CTGCCTTTCA TCCTGTATGT CAGGTGATTG CTATGTATGA CTATGCAGCA 
AATAATGAAG ATGAGCTCAG TTTCTCCAAG GGACAACTCA TTAATGTTAT GAACAAAGAT 
GATCCTGATT GGTGGCAAGG AGAGATCAAC GGGGTGACTG GTCTCTTTCC TTCAAACTAC 
GTTAAGATGA CGACAGACTC AGATCCAAGT CAACAGTGGT GTGCTGATCT GCAAACCCTG 
GACACAATGC AGCCAATTGA GAGGAAAAGA CAGGGCTATA TTCATGAGCT GATTCAGACC 
GAAGAGCGGT ACATGGCTGA CCTTCAGCTC GTCGTCGAGG TTTTTCAGAA ACGCATGGCA 
GAGTCAGGCT TTCTCACTGA AGGGGAGATG GCCCTGATTT TTGTTAACTG GAAGGAGCTC 
ATCATGTCCA ACACAAAGCT GCTGAAGGCT TTGCGGGTGC GGAAGAAGAC CGGGGGCGAG 
AAGATGCCGG TGCAGATGAT TGGGGACATC CTGGCCGCTG AGCTGTCCCA CATGCAGGCT 
TACATCAGGT TCTGCAGCTG CCAGCTTAAT GGAGCAGCTC TGTTACAGCA GAAGACAGAT 
GAAGACACAG ATTTCAAAGA ATTTTTAAAG AAGCTGGCAT CTGACCCGCG GTGTAAAGGA 
ATGCCCCTCT CCAGCTTCCT GCTGAAACCC ATGCAGAGGA TCACCCGCTA CCCACTGCTC 
ATCAGAAGTA TTCTGGAGAA CACCCCGGAG AGCCATGCAG ACCATTCCTC CCTAAAGCTG 
GCCCTCGAGC GGGCAGAGGA GCTGTGCTCT CAAGTGAATG AGGGAGTTCG GGAGAAGGAA 
AACTCGGACC GACTGGAGTG GATCCAGGCG CACGTGCAGT GTGAAGGCCT CGCGGAGCAA 
CTTATTTTCA ACTCTCTCAC CAACTGCCTG GGGCCCCGGA AGCTCTTACA CAGTGGGAAA 
TTATACAAGA CCAAGAGCAA CAAGGAACTG CACGGATTCC TCTTCAATGA CTTCCTGCTT 
CTTACCTACA TGGTCAAGCA GTTTGCTGTT TCCTCTGGCT CTGAGAAACT TTTCAGCTCG 
AAGTCCAATG CTCAATTCAA AATGTATAAA ACGCCCATTT TCCTGAATGA AGTCTTGGTG 
AAACTGCCCA CAGACCCTTC CAGCGATGAG CCTGTCTTCC ACATTTCCCA CATTGATCGG 
GTCTACACCC TCCGAACAGA CAACATTAAT GAGAGGACCG CCTGGGTGCA GAAGATCAAG 
GCGGCGTCTG AGCAGTACAT CGACACCGAG AAGAAGAAGC GTGAGAAAGC TTACCAAGCC 
CGCTCCCAAA AGACTTCAGG CATTGGGCGC CTGATGGTGC ATGTCATTGA AGCTACAGAA 
TTAAAAGCCT GCAAACCAAA TGGAAAGAGC AACCCATACT GTGAAATCAG CATGGGCTCC 
CAGAGCTACA CCACCAGGAC CATCCAGGAC ACACTCAATC CCAAGTGGAA TTTTAACTGC 
CAGTTCTTTA TTAAGGATCT CTACCAAGAC GTGCTGTGTC TCACCCTGTT TGACAGAGAC 
CAGTTTTCAC CAGATGATTT CCTGGGTCGT ACTGAAATTC CAGTGGCAAA AATTCGAACA 
GAACAGGAAA GCAAAGGCCC TATGACCCGC CGACTGCTGC TGCATGAGGT CCCCACCGGG 
GAGGTCTGGG TCCGTTTTGA CCTGCAGCTT TTTGAGCAAA AAACTCTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.
Biochemical and biophysical research communications484(4)813-819(2017 03)
Kropyvko S,Gryaznova T,Morderer D,Rynditch A

ITSN2L Interacts with and Negatively Regulates RABEP1.
International journal of molecular sciences16(12)28242-54(2015 Nov)
Yang X,Yan F,He Z,Liu S,Cheng Y,Wei K,Gan S,Yuan J,Wang S,Xiao Y,Ren K,Liu N,Hu X,Ding X,Hu X,Xiang S

Genome-wide association study identifies multiple susceptibility loci for diffuse large B cell lymphoma.
Nature genetics46(11)1233-8(2014 Nov)
Cerhan JR,Berndt SI,Vijai J,Ghesqui?res H,McKay J,Wang SS,Wang Z,Yeager M,Conde L,de Bakker PI,Nieters A,Cox D,Burdett L,Monnereau A,Flowers CR,De Roos AJ,Brooks-Wilson AR,Lan Q,Severi G,Melbye M,Gu J,Jackson RD,Kane E,Teras LR,Purdue MP,Vajdic CM,Spinelli JJ,Giles GG,Albanes D,Kelly RS,Zucca M,Bertrand KA,Zeleniuch-Jacquotte A,Lawrence C,Hutchinson A,Zhi D,Habermann TM,Link BK,Novak AJ,Dogan A,Asmann YW,Liebow M,Thompson CA,Ansell SM,Witzig TE,Weiner GJ,Veron AS,Zelenika D,Tilly H,Haioun C,Molina TJ,Hjalgrim H,Glimelius B,Adami HO,Bracci PM,Riby J,Smith MT,Holly EA,Cozen W,Hartge P,Morton LM,Severson RK,Tinker LF,North KE,Becker N,Benavente Y,Boffetta P,Brennan P,Foretova L,Maynadie M,Staines A,Lightfoot T,Crouch S,Smith A,Roman E,Diver WR,Offit K,Zelenetz A,Klein RJ,Villano DJ,Zheng T,Zhang Y,Holford TR,Kricker A,Turner J,Southey MC,Clavel J,Virtamo J,Weinstein S,Riboli E,Vineis P,Kaaks R,Trichopoulos D,Vermeulen RC,Boeing H,Tjonneland A,Angelucci E,Di Lollo S,Rais M,Birmann BM,Laden F,Giovannucci E,Kraft P,Huang J,Ma B,Ye Y,Chiu BC,Sampson J,Liang L,Park JH,Chung CC,Weisenburger DD,Chatterjee N,Fraumeni JF Jr,Slager SL,Wu X,de Sanjose S,Smedby KE,Salles G,Skibola CF,Rothman N,Chanock SJ

SYT14L, especially its C2 domain, is involved in regulating melanocyte differentiation.
Journal of dermatological science72(3)246-51(2013 Dec)
Yoo JC,Lim Ty,Park JS,Hah YS,Park N,Hong SG,Park JY,Yoon TJ

Adaptor proteins intersectin 1 and 2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins.
PloS one8(7)e70546(2013)
Novokhatska O,Dergai M,Tsyba L,Skrypkina I,Filonenko V,Moreau J,Rynditch A

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ITSN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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