ATP4A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP4A
Entrez Gene ID	495
Full Name	ATPase H+/K+ transporting alpha subunit
Synonyms	ATP6A
General protein information	Preferred Names ATPase H+/K+ transporting alpha subunit Names potassium-transporting ATPase alpha chain 1 ATPase, H+/K+ exchanging, alpha polypeptide ATPase, H+/K+ transporting, alpha polypeptide gastric H+/K+ ATPase alpha subunit gastric H,K-ATPase catalytic subunit gastric hydrogen-potassium ATPase proton pump
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008].
Disorder MIM:	137216

mRNA and Protein(s)

mRNA	Protein	Name
NM_000704.3	NP_000695.2	potassium-transporting ATPase alpha chain 1
NM_000704.2	NP_000695.2	potassium-transporting ATPase alpha chain 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atp4a	NP_061201.2
Pan troglodytes (chimpanzee)	ATP4A	XP_003953499.1
Homo sapiens (human)	ATP4A	NP_000695.2
Rattus norvegicus (Norway rat)	Atp4a	NP_036641.1
Canis lupus familiaris (dog)	ATP4A	NP_001003342.1
Bos taurus (cattle)	ATP4A	NP_001137561.1

Gene Ontology
Bibliography

Related articles in PubMed

A cumulative effect involving malfunction of the PTH1R and ATP4A genes explains a familial gastric neuroendocrine tumor with hypothyroidism and arthritis.
Calvete O, Herraiz M, Reyes J, Pati?o A, Benitez J
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association20(6)998-1003(2017 Nov)

ATP4A autoimmunity in pediatric patients with type 1 diabetes and its relationship to blood count, iron metabolism, and vitamin B12.
Chobot A, Rusak E, Wenzlau J, Davidson H, Adamczyk P, Krzywicka A, Mazur B, Pola?ska J, Rewers M
Pediatric diabetes(2017 Apr)

A knockin mouse model for human ATP4aR703C mutation identified in familial gastric neuroendocrine tumors recapitulates the premalignant condition of the human disease and suggests new therapeutic strategies.
Calvete O, Varro A, Pritchard DM, Barroso A, Oteo M, Morcillo M?, Vargiu P, Dodd S, Garcia M, Reyes J, Ortega S, Benitez J
Disease models & mechanisms9(9)975-84(2016 09)

ECL-cell carcinoids and carcinoma in patients homozygous for an inactivating mutation in the gastric H(+) K(+) ATPase alpha subunit.
Fossmark R, Calvete O, Mj?nes P, Benitez J, Waldum HL
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica124(7)561-6(2016 Jul)

Exome sequencing identifies ATP4A gene as responsible of an atypical familial type I gastric neuroendocrine tumour.
Calvete O, Reyes J, Zu?iga S, Paumard-Hern?ndez B, Fern?ndez V, Bujanda L, Rodriguez-Pinilla MS, Palacios J, Heine-Su?er D, Banka S, Newman WG, Ca?amero M, Pritchard DM, Ben?tez J
Human molecular genetics24(10)2914-22(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the generation and characterization of a knockin mouse model for the ATP4a(R703C) mutation to better understand the tumorigenesis process.
Title: A knockin mouse model for human ATP4aR703C mutation identified in familial gastric neuroendocrine tumors recapitulates the premalignant condition of the human disease and suggests new therapeutic strategies.

In conclusion, patients homozygous for an inactivating ATP4A mutation develop gastric ECL-cell carcinoids in their 3rd or 4th decade.
Title: ECL-cell carcinoids and carcinoma in patients homozygous for an inactivating mutation in the gastric H(+) K(+) ATPase alpha subunit.

Homozygous missense mutation in the 14th exon of the ATP4A gene (c.2107C>T) is responsible for an atypical familial type I gastric neuroendocrine tumour.
Title: Exome sequencing identifies ATP4A gene as responsible of an atypical familial type I gastric neuroendocrine tumour.

ATP4A are found in nearly one-third of children with type 1 diabetes and more common among females. In this cross-sectional analysis, Hp infection was not associated with autoimmunity against parietal cells.
Title: ATP4A autoimmunity and Helicobacter pylori infection in children with type 1 diabetes.

Downregulation of ATP4A gene is associated with gastric cancer.
Title: Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.

The following ATP4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25737	NM_000704.3 Latest version!	Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu25737	NM_000704.2	Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP4A ( NM_000704.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000695.2 Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.

Price & Availability↑

CloneID	OHu25737
Clone ID Related Accession (Same CDS sequence)	NM_000704.3 , NM_000704.2
Accession Version	NM_000704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	potassium-transporting ATPase alpha chain 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058032.1, AL832971.1 and AC002389.1. On May 17, 2019 this sequence version replaced NM_000704.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ891529.1, AK223259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262623.4/ ENSP00000262623.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CCGAGAACTA TGAGCTCTAC TCGGTGGAGC TGGGTCCTGG CCCTGGCGGG 
GACATGGCTG CCAAGATGAG CAAGAAGAAG AAGGCGGGTG GCGGGGGTGG CAAGAGGAAG 
GAGAAGCTGG AGAACATGAA GAAGGAGATG GAGATTAACG ACCACCAGCT GTCAGTGGCG 
GAGCTGGAAC AGAAATACCA GACCAGTGCC ACCAAGGGCC TCTCTGCGAG CCTGGCTGCT 
GAGCTGCTGC TGCGGGATGG GCCCAACGCA CTGCGGCCAC CACGGGGCAC CCCAGAGTAC 
GTCAAGTTCG CGAGGCAGCT GGCCGGGGGC CTGCAGTGCC TCATGTGGGT TGCCGCCGCC 
ATCTGCCTCA TCGCCTTTGC CATCCAGGCT AGTGAGGGGG ACCTCACCAC CGACGACAAT 
CTGTACCTGG CAATCGCTCT CATTGCTGTG GTTGTCGTCA CCGGCTGCTT TGGCTACTAC 
CAGGAATTCA AGAGCACCAA CATCATCGCC AGCTTTAAGA ACCTTGTGCC ACAGCAAGCC 
ACTGTCATCC GCGATGGAGA CAAATTCCAG ATCAACGCTG ACCAACTGGT GGTGGGCGAC 
CTGGTGGAGA TGAAAGGTGG GGACAGAGTG CCCGCCGACA TCCGCATCCT GGCGGCCCAG 
GGCTGCAAGG TGGACAACTC CTCGCTGACA GGGGAGTCTG AGCCACAGAC CCGCTCACCC 
GAGTGCACGC ACGAGAGCCC TCTGGAGACC CGCAACATCG CCTTCTTCTC CACCATGTGC 
CTTGAGGGCA CCGTGCAGGG CCTGGTGGTG AACACGGGCG ACCGCACCAT CATTGGGCGC 
ATCGCATCGC TGGCGTCGGG GGTGGAAAAC GAGAAGACAC CCATCGCTAT CGAGATCGAG 
CATTTTGTGG ACATCATCGC GGGCCTGGCC ATTCTCTTCG GTGCCACATT TTTTATTGTG 
GCCATGTGCA TTGGCTACAC CTTCCTGCGG GCCATGGTCT TCTTCATGGC CATCGTGGTG 
GCCTATGTGC CTGAGGGGCT GCTGGCCACT GTCACAGTCT GCCTGTCCCT GACAGCCAAG 
CGCCTGGCCA GTAAGAACTG CGTGGTCAAG AACCTGGAGG CGGTGGAGAC ATTGGGCTCC 
ACTTCGGTGA TCTGCTCGGA CAAGACAGGG ACTCTCACTC AGAACCGCAT GACTGTGTCC 
CATCTGTGGT TTGACAACCA CATCCACACA GCTGACACCA CGGAAGACCA GTCAGGGCAG 
ACGTTTGACC AGTCCTCGGA GACGTGGCGG GCGCTGTGCC GGGTGCTCAC CCTGTGCAAC 
CGCGCCGCCT TCAAGTCCGG CCAGGATGCA GTGCCTGTGC CCAAGCGCAT CGTGATTGGA 
GACGCATCGG AGACGGCGCT GCTCAAGTTC TCGGAGCTGA CGCTGGGCAA CGCCATGGGC 
TACCGGGACC GCTTCCCAAA AGTCTGCGAG ATACCCTTCA ACTCCACCAA CAAGTTCCAG 
CTGTCCATCC ATACGCTGGA GGACCCGCGG GACCCGCGAC ACTTGCTGGT GATGAAGGGC 
GCCCCCGAGC GCGTGCTGGA GCGCTGCAGC TCCATCCTTA TCAAGGGCCA GGAGCTGCCG 
CTGGACGAGC AGTGGCGCGA GGCCTTCCAG ACCGCCTACC TCAGCCTGGG AGGCCTGGGC 
GAACGCGTGC TCGGCTTCTG CCAGCTCTAC CTGAATGAGA AGGACTACCC GCCTGGCTAT 
GCCTTCGACG TAGAGGCCAT GAACTTTCCA TCTAGCGGCC TCTGCTTTGC GGGACTTGTA 
TCCATGATTG ACCCACCCCG GGCCACCGTC CCTGATGCTG TGCTCAAGTG TCGCACCGCA 
GGCATCCGGG TGATCATGGT AACGGGTGAC CACCCCATCA CCGCCAAGGC CATTGCAGCC 
AGTGTGGGCA TCATCTCGGA AGGCAGCGAG ACAGTGGAGG ACATCGCTGC CCGCCTCCGT 
GTGCCCGTAG ACCAGGTTAA TCGCAAGGAT GCCCGTGCCT GTGTGATCAA TGGCATGCAG 
CTGAAGGACA TGGACCCATC GGAACTGGTC GAGGCCCTGC GCACCCACCC CGAGATGGTG 
TTTGCGCGCA CCAGCCCCCA GCAGAAGCTG GTGATCGTGG AGAGCTGCCA GCGGCTGGGT 
GCGATTGTGG CCGTCACGGG GGATGGTGTG AATGACTCCC CAGCTCTGAA GAAGGCAGAC 
ATCGGAGTAG CCATGGGCAT CGCTGGCTCA GATGCTGCCA AAAATGCAGC TGACATGATC 
CTGCTGGATG ACAACTTTGC CTCCATTGTG ACAGGCGTGG AGCAGGGTCG ACTGATCTTC 
GACAACCTGA AGAAGTCTAT TGCCTACACA TTGACCAAGA ACATCCCAGA GCTGACACCC 
TACCTCATCT ACATCACCGT CAGCGTGCCC CTGCCCCTCG GGTGCATCAC CATCCTCTTC 
ATCGAACTCT GCACTGACAT TTTCCCATCT GTGTCCCTGG CATATGAAAA GGCCGAGAGT 
GACATCATGC ACCTGCGTCC ACGCAACCCA AAGCGTGACA GATTGGTCAA CGAGCCCCTG 
GCTGCCTACT CCTACTTCCA GATTGGTGCC ATTCAGTCCT TTGCTGGCTT CACTGACTAC 
TTCACGGCAA TGGCCCAGGA GGGCTGGTTC CCACTGCTGT GCGTGGGGCT GCGGGCGCAG 
TGGGAGGACC ACCACCTACA AGATCTGCAG GACAGCTACG GCCAGGAGTG GACATTCGGG 
CAGCGCCTGT ACCAGCAGTA CACCTGCTAC ACCGTGTTCT TCATCAGCAT TGAGGTGTGC 
CAGATCGCCG ATGTCCTCAT CCGCAAGACG CGCCGTCTCT CTGCCTTCCA GCAAGGCTTC 
TTCAGGAATA AGATCCTGGT GATCGCCATC GTGTTCCAGG TCTGCATCGG CTGCTTCCTG 
TGCTACTGCC CCGGCATGCC CAACATCTTC AACTTCATGC CCATTCGGTT CCAGTGGTGG 
CTGGTCCCCC TGCCCTACGG CATCCTCATC TTCGTCTATG ATGAGATCCG GAAGCTTGGA 
GTTCGCTGTT GCCCAGGGAG CTGGTGGGAC CAGGAACTCT ACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000695.2
CDS	30..3137
Translation	MGKAENYELYSVELGPGPGGDMAAKMSKKKKAGGGGGKRKEKLENMKKEMEINDHQLSVAELEQKYQTSATKGLSASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDNLYLAIALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRVPADIRILAAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTVQGLVVNTGDRTIIGRIASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATVTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQTFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEIPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDHPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMVFARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVTGVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAESDIMHLRPRNPKRDRLVNEPLAAYSYFQIGAIQSFAGFTDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQDSYGQEWTFGQRLYQQYTCYTVFFISIEVCQIADVLIRKTRRLSAFQQGFFRNKILVIAIVFQVCIGCFLCYCPGMPNIFNFMPIRFQWWLVPLPYGILIFVYDEIRKLGVRCCPGSWWDQELYY

Target ORF information:

RefSeq Version	NM_000704.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000704.3

ORF Insert Sequence:

ATGGGGAAGG CCGAGAACTA TGAGCTCTAC TCGGTGGAGC TGGGTCCTGG CCCTGGCGGG 
GACATGGCTG CCAAGATGAG CAAGAAGAAG AAGGCGGGTG GCGGGGGTGG CAAGAGGAAG 
GAGAAGCTGG AGAACATGAA GAAGGAGATG GAGATTAACG ACCACCAGCT GTCAGTGGCG 
GAGCTGGAAC AGAAATACCA GACCAGTGCC ACCAAGGGCC TCTCTGCGAG CCTGGCTGCT 
GAGCTGCTGC TGCGGGATGG GCCCAACGCA CTGCGGCCAC CACGGGGCAC CCCAGAGTAC 
GTCAAGTTCG CGAGGCAGCT GGCCGGGGGC CTGCAGTGCC TCATGTGGGT TGCCGCCGCC 
ATCTGCCTCA TCGCCTTTGC CATCCAGGCT AGTGAGGGGG ACCTCACCAC CGACGACAAT 
CTGTACCTGG CAATCGCTCT CATTGCTGTG GTTGTCGTCA CCGGCTGCTT TGGCTACTAC 
CAGGAATTCA AGAGCACCAA CATCATCGCC AGCTTTAAGA ACCTTGTGCC ACAGCAAGCC 
ACTGTCATCC GCGATGGAGA CAAATTCCAG ATCAACGCTG ACCAACTGGT GGTGGGCGAC 
CTGGTGGAGA TGAAAGGTGG GGACAGAGTG CCCGCCGACA TCCGCATCCT GGCGGCCCAG 
GGCTGCAAGG TGGACAACTC CTCGCTGACA GGGGAGTCTG AGCCACAGAC CCGCTCACCC 
GAGTGCACGC ACGAGAGCCC TCTGGAGACC CGCAACATCG CCTTCTTCTC CACCATGTGC 
CTTGAGGGCA CCGTGCAGGG CCTGGTGGTG AACACGGGCG ACCGCACCAT CATTGGGCGC 
ATCGCATCGC TGGCGTCGGG GGTGGAAAAC GAGAAGACAC CCATCGCTAT CGAGATCGAG 
CATTTTGTGG ACATCATCGC GGGCCTGGCC ATTCTCTTCG GTGCCACATT TTTTATTGTG 
GCCATGTGCA TTGGCTACAC CTTCCTGCGG GCCATGGTCT TCTTCATGGC CATCGTGGTG 
GCCTATGTGC CTGAGGGGCT GCTGGCCACT GTCACAGTCT GCCTGTCCCT GACAGCCAAG 
CGCCTGGCCA GTAAGAACTG CGTGGTCAAG AACCTGGAGG CGGTGGAGAC ATTGGGCTCC 
ACTTCGGTGA TCTGCTCGGA CAAGACAGGG ACTCTCACTC AGAACCGCAT GACTGTGTCC 
CATCTGTGGT TTGACAACCA CATCCACACA GCTGACACCA CGGAAGACCA GTCAGGGCAG 
ACGTTTGACC AGTCCTCGGA GACGTGGCGG GCGCTGTGCC GGGTGCTCAC CCTGTGCAAC 
CGCGCCGCCT TCAAGTCCGG CCAGGATGCA GTGCCTGTGC CCAAGCGCAT CGTGATTGGA 
GACGCATCGG AGACGGCGCT GCTCAAGTTC TCGGAGCTGA CGCTGGGCAA CGCCATGGGC 
TACCGGGACC GCTTCCCAAA AGTCTGCGAG ATACCCTTCA ACTCCACCAA CAAGTTCCAG 
CTGTCCATCC ATACGCTGGA GGACCCGCGG GACCCGCGAC ACTTGCTGGT GATGAAGGGC 
GCCCCCGAGC GCGTGCTGGA GCGCTGCAGC TCCATCCTTA TCAAGGGCCA GGAGCTGCCG 
CTGGACGAGC AGTGGCGCGA GGCCTTCCAG ACCGCCTACC TCAGCCTGGG AGGCCTGGGC 
GAACGCGTGC TCGGCTTCTG CCAGCTCTAC CTGAATGAGA AGGACTACCC GCCTGGCTAT 
GCCTTCGACG TAGAGGCCAT GAACTTTCCA TCTAGCGGCC TCTGCTTTGC GGGACTTGTA 
TCCATGATTG ACCCACCCCG GGCCACCGTC CCTGATGCTG TGCTCAAGTG TCGCACCGCA 
GGCATCCGGG TGATCATGGT AACGGGTGAC CACCCCATCA CCGCCAAGGC CATTGCAGCC 
AGTGTGGGCA TCATCTCGGA AGGCAGCGAG ACAGTGGAGG ACATCGCTGC CCGCCTCCGT 
GTGCCCGTAG ACCAGGTTAA TCGCAAGGAT GCCCGTGCCT GTGTGATCAA TGGCATGCAG 
CTGAAGGACA TGGACCCATC GGAACTGGTC GAGGCCCTGC GCACCCACCC CGAGATGGTG 
TTTGCGCGCA CCAGCCCCCA GCAGAAGCTG GTGATCGTGG AGAGCTGCCA GCGGCTGGGT 
GCGATTGTGG CCGTCACGGG GGATGGTGTG AATGACTCCC CAGCTCTGAA GAAGGCAGAC 
ATCGGAGTAG CCATGGGCAT CGCTGGCTCA GATGCTGCCA AAAATGCAGC TGACATGATC 
CTGCTGGATG ACAACTTTGC CTCCATTGTG ACAGGCGTGG AGCAGGGTCG ACTGATCTTC 
GACAACCTGA AGAAGTCTAT TGCCTACACA TTGACCAAGA ACATCCCAGA GCTGACACCC 
TACCTCATCT ACATCACCGT CAGCGTGCCC CTGCCCCTCG GGTGCATCAC CATCCTCTTC 
ATCGAACTCT GCACTGACAT TTTCCCATCT GTGTCCCTGG CATATGAAAA GGCCGAGAGT 
GACATCATGC ACCTGCGTCC ACGCAACCCA AAGCGTGACA GATTGGTCAA CGAGCCCCTG 
GCTGCCTACT CCTACTTCCA GATTGGTGCC ATTCAGTCCT TTGCTGGCTT CACTGACTAC 
TTCACGGCAA TGGCCCAGGA GGGCTGGTTC CCACTGCTGT GCGTGGGGCT GCGGGCGCAG 
TGGGAGGACC ACCACCTACA AGATCTGCAG GACAGCTACG GCCAGGAGTG GACATTCGGG 
CAGCGCCTGT ACCAGCAGTA CACCTGCTAC ACCGTGTTCT TCATCAGCAT TGAGGTGTGC 
CAGATCGCCG ATGTCCTCAT CCGCAAGACG CGCCGTCTCT CTGCCTTCCA GCAAGGCTTC 
TTCAGGAATA AGATCCTGGT GATCGCCATC GTGTTCCAGG TCTGCATCGG CTGCTTCCTG 
TGCTACTGCC CCGGCATGCC CAACATCTTC AACTTCATGC CCATTCGGTT CCAGTGGTGG 
CTGGTCCCCC TGCCCTACGG CATCCTCATC TTCGTCTATG ATGAGATCCG GAAGCTTGGA 
GTTCGCTGTT GCCCAGGGAG CTGGTGGGAC CAGGAACTCT ACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP4A ( NM_000704.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000695.2 Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.

Price & Availability↑

CloneID	OHu25737
Clone ID Related Accession (Same CDS sequence)	NM_000704.3 , NM_000704.2
Accession Version	NM_000704.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	potassium-transporting ATPase alpha chain 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058032.1 and AL832971.1. On Sep 8, 2004 this sequence version replaced NM_000704.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223259.1, AK058032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CCGAGAACTA TGAGCTCTAC TCGGTGGAGC TGGGTCCTGG CCCTGGCGGG 
GACATGGCTG CCAAGATGAG CAAGAAGAAG AAGGCGGGTG GCGGGGGTGG CAAGAGGAAG 
GAGAAGCTGG AGAACATGAA GAAGGAGATG GAGATTAACG ACCACCAGCT GTCAGTGGCG 
GAGCTGGAAC AGAAATACCA GACCAGTGCC ACCAAGGGCC TCTCTGCGAG CCTGGCTGCT 
GAGCTGCTGC TGCGGGATGG GCCCAACGCA CTGCGGCCAC CACGGGGCAC CCCAGAGTAC 
GTCAAGTTCG CGAGGCAGCT GGCCGGGGGC CTGCAGTGCC TCATGTGGGT TGCCGCCGCC 
ATCTGCCTCA TCGCCTTTGC CATCCAGGCT AGTGAGGGGG ACCTCACCAC CGACGACAAT 
CTGTACCTGG CAATCGCTCT CATTGCTGTG GTTGTCGTCA CCGGCTGCTT TGGCTACTAC 
CAGGAATTCA AGAGCACCAA CATCATCGCC AGCTTTAAGA ACCTTGTGCC ACAGCAAGCC 
ACTGTCATCC GCGATGGAGA CAAATTCCAG ATCAACGCTG ACCAACTGGT GGTGGGCGAC 
CTGGTGGAGA TGAAAGGTGG GGACAGAGTG CCCGCCGACA TCCGCATCCT GGCGGCCCAG 
GGCTGCAAGG TGGACAACTC CTCGCTGACA GGGGAGTCTG AGCCACAGAC CCGCTCACCC 
GAGTGCACGC ACGAGAGCCC TCTGGAGACC CGCAACATCG CCTTCTTCTC CACCATGTGC 
CTTGAGGGCA CCGTGCAGGG CCTGGTGGTG AACACGGGCG ACCGCACCAT CATTGGGCGC 
ATCGCATCGC TGGCGTCGGG GGTGGAAAAC GAGAAGACAC CCATCGCTAT CGAGATCGAG 
CATTTTGTGG ACATCATCGC GGGCCTGGCC ATTCTCTTCG GTGCCACATT TTTTATTGTG 
GCCATGTGCA TTGGCTACAC CTTCCTGCGG GCCATGGTCT TCTTCATGGC CATCGTGGTG 
GCCTATGTGC CTGAGGGGCT GCTGGCCACT GTCACAGTCT GCCTGTCCCT GACAGCCAAG 
CGCCTGGCCA GTAAGAACTG CGTGGTCAAG AACCTGGAGG CGGTGGAGAC ATTGGGCTCC 
ACTTCGGTGA TCTGCTCGGA CAAGACAGGG ACTCTCACTC AGAACCGCAT GACTGTGTCC 
CATCTGTGGT TTGACAACCA CATCCACACA GCTGACACCA CGGAAGACCA GTCAGGGCAG 
ACGTTTGACC AGTCCTCGGA GACGTGGCGG GCGCTGTGCC GGGTGCTCAC CCTGTGCAAC 
CGCGCCGCCT TCAAGTCCGG CCAGGATGCA GTGCCTGTGC CCAAGCGCAT CGTGATTGGA 
GACGCATCGG AGACGGCGCT GCTCAAGTTC TCGGAGCTGA CGCTGGGCAA CGCCATGGGC 
TACCGGGACC GCTTCCCAAA AGTCTGCGAG ATACCCTTCA ACTCCACCAA CAAGTTCCAG 
CTGTCCATCC ATACGCTGGA GGACCCGCGG GACCCGCGAC ACTTGCTGGT GATGAAGGGC 
GCCCCCGAGC GCGTGCTGGA GCGCTGCAGC TCCATCCTTA TCAAGGGCCA GGAGCTGCCG 
CTGGACGAGC AGTGGCGCGA GGCCTTCCAG ACCGCCTACC TCAGCCTGGG AGGCCTGGGC 
GAACGCGTGC TCGGCTTCTG CCAGCTCTAC CTGAATGAGA AGGACTACCC GCCTGGCTAT 
GCCTTCGACG TAGAGGCCAT GAACTTTCCA TCTAGCGGCC TCTGCTTTGC GGGACTTGTA 
TCCATGATTG ACCCACCCCG GGCCACCGTC CCTGATGCTG TGCTCAAGTG TCGCACCGCA 
GGCATCCGGG TGATCATGGT AACGGGTGAC CACCCCATCA CCGCCAAGGC CATTGCAGCC 
AGTGTGGGCA TCATCTCGGA AGGCAGCGAG ACAGTGGAGG ACATCGCTGC CCGCCTCCGT 
GTGCCCGTAG ACCAGGTTAA TCGCAAGGAT GCCCGTGCCT GTGTGATCAA TGGCATGCAG 
CTGAAGGACA TGGACCCATC GGAACTGGTC GAGGCCCTGC GCACCCACCC CGAGATGGTG 
TTTGCGCGCA CCAGCCCCCA GCAGAAGCTG GTGATCGTGG AGAGCTGCCA GCGGCTGGGT 
GCGATTGTGG CCGTCACGGG GGATGGTGTG AATGACTCCC CAGCTCTGAA GAAGGCAGAC 
ATCGGAGTAG CCATGGGCAT CGCTGGCTCA GATGCTGCCA AAAATGCAGC TGACATGATC 
CTGCTGGATG ACAACTTTGC CTCCATTGTG ACAGGCGTGG AGCAGGGTCG ACTGATCTTC 
GACAACCTGA AGAAGTCTAT TGCCTACACA TTGACCAAGA ACATCCCAGA GCTGACACCC 
TACCTCATCT ACATCACCGT CAGCGTGCCC CTGCCCCTCG GGTGCATCAC CATCCTCTTC 
ATCGAACTCT GCACTGACAT TTTCCCATCT GTGTCCCTGG CATATGAAAA GGCCGAGAGT 
GACATCATGC ACCTGCGTCC ACGCAACCCA AAGCGTGACA GATTGGTCAA CGAGCCCCTG 
GCTGCCTACT CCTACTTCCA GATTGGTGCC ATTCAGTCCT TTGCTGGCTT CACTGACTAC 
TTCACGGCAA TGGCCCAGGA GGGCTGGTTC CCACTGCTGT GCGTGGGGCT GCGGGCGCAG 
TGGGAGGACC ACCACCTACA AGATCTGCAG GACAGCTACG GCCAGGAGTG GACATTCGGG 
CAGCGCCTGT ACCAGCAGTA CACCTGCTAC ACCGTGTTCT TCATCAGCAT TGAGGTGTGC 
CAGATCGCCG ATGTCCTCAT CCGCAAGACG CGCCGTCTCT CTGCCTTCCA GCAAGGCTTC 
TTCAGGAATA AGATCCTGGT GATCGCCATC GTGTTCCAGG TCTGCATCGG CTGCTTCCTG 
TGCTACTGCC CCGGCATGCC CAACATCTTC AACTTCATGC CCATTCGGTT CCAGTGGTGG 
CTGGTCCCCC TGCCCTACGG CATCCTCATC TTCGTCTATG ATGAGATCCG GAAGCTTGGA 
GTTCGCTGTT GCCCAGGGAG CTGGTGGGAC CAGGAACTCT ACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000695.2
CDS	30..3137
Translation	MGKAENYELYSVELGPGPGGDMAAKMSKKKKAGGGGGKRKEKLENMKKEMEINDHQLSVAELEQKYQTSATKGLSASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDNLYLAIALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRVPADIRILAAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTVQGLVVNTGDRTIIGRIASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATVTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQTFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEIPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDHPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMVFARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVTGVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAESDIMHLRPRNPKRDRLVNEPLAAYSYFQIGAIQSFAGFTDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQDSYGQEWTFGQRLYQQYTCYTVFFISIEVCQIADVLIRKTRRLSAFQQGFFRNKILVIAIVFQVCIGCFLCYCPGMPNIFNFMPIRFQWWLVPLPYGILIFVYDEIRKLGVRCCPGSWWDQELYY

Target ORF information:

RefSeq Version	NM_000704.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+/K+ transporting subunit alpha (ATP4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000704.2

ORF Insert Sequence:

ATGGGGAAGG CCGAGAACTA TGAGCTCTAC TCGGTGGAGC TGGGTCCTGG CCCTGGCGGG 
GACATGGCTG CCAAGATGAG CAAGAAGAAG AAGGCGGGTG GCGGGGGTGG CAAGAGGAAG 
GAGAAGCTGG AGAACATGAA GAAGGAGATG GAGATTAACG ACCACCAGCT GTCAGTGGCG 
GAGCTGGAAC AGAAATACCA GACCAGTGCC ACCAAGGGCC TCTCTGCGAG CCTGGCTGCT 
GAGCTGCTGC TGCGGGATGG GCCCAACGCA CTGCGGCCAC CACGGGGCAC CCCAGAGTAC 
GTCAAGTTCG CGAGGCAGCT GGCCGGGGGC CTGCAGTGCC TCATGTGGGT TGCCGCCGCC 
ATCTGCCTCA TCGCCTTTGC CATCCAGGCT AGTGAGGGGG ACCTCACCAC CGACGACAAT 
CTGTACCTGG CAATCGCTCT CATTGCTGTG GTTGTCGTCA CCGGCTGCTT TGGCTACTAC 
CAGGAATTCA AGAGCACCAA CATCATCGCC AGCTTTAAGA ACCTTGTGCC ACAGCAAGCC 
ACTGTCATCC GCGATGGAGA CAAATTCCAG ATCAACGCTG ACCAACTGGT GGTGGGCGAC 
CTGGTGGAGA TGAAAGGTGG GGACAGAGTG CCCGCCGACA TCCGCATCCT GGCGGCCCAG 
GGCTGCAAGG TGGACAACTC CTCGCTGACA GGGGAGTCTG AGCCACAGAC CCGCTCACCC 
GAGTGCACGC ACGAGAGCCC TCTGGAGACC CGCAACATCG CCTTCTTCTC CACCATGTGC 
CTTGAGGGCA CCGTGCAGGG CCTGGTGGTG AACACGGGCG ACCGCACCAT CATTGGGCGC 
ATCGCATCGC TGGCGTCGGG GGTGGAAAAC GAGAAGACAC CCATCGCTAT CGAGATCGAG 
CATTTTGTGG ACATCATCGC GGGCCTGGCC ATTCTCTTCG GTGCCACATT TTTTATTGTG 
GCCATGTGCA TTGGCTACAC CTTCCTGCGG GCCATGGTCT TCTTCATGGC CATCGTGGTG 
GCCTATGTGC CTGAGGGGCT GCTGGCCACT GTCACAGTCT GCCTGTCCCT GACAGCCAAG 
CGCCTGGCCA GTAAGAACTG CGTGGTCAAG AACCTGGAGG CGGTGGAGAC ATTGGGCTCC 
ACTTCGGTGA TCTGCTCGGA CAAGACAGGG ACTCTCACTC AGAACCGCAT GACTGTGTCC 
CATCTGTGGT TTGACAACCA CATCCACACA GCTGACACCA CGGAAGACCA GTCAGGGCAG 
ACGTTTGACC AGTCCTCGGA GACGTGGCGG GCGCTGTGCC GGGTGCTCAC CCTGTGCAAC 
CGCGCCGCCT TCAAGTCCGG CCAGGATGCA GTGCCTGTGC CCAAGCGCAT CGTGATTGGA 
GACGCATCGG AGACGGCGCT GCTCAAGTTC TCGGAGCTGA CGCTGGGCAA CGCCATGGGC 
TACCGGGACC GCTTCCCAAA AGTCTGCGAG ATACCCTTCA ACTCCACCAA CAAGTTCCAG 
CTGTCCATCC ATACGCTGGA GGACCCGCGG GACCCGCGAC ACTTGCTGGT GATGAAGGGC 
GCCCCCGAGC GCGTGCTGGA GCGCTGCAGC TCCATCCTTA TCAAGGGCCA GGAGCTGCCG 
CTGGACGAGC AGTGGCGCGA GGCCTTCCAG ACCGCCTACC TCAGCCTGGG AGGCCTGGGC 
GAACGCGTGC TCGGCTTCTG CCAGCTCTAC CTGAATGAGA AGGACTACCC GCCTGGCTAT 
GCCTTCGACG TAGAGGCCAT GAACTTTCCA TCTAGCGGCC TCTGCTTTGC GGGACTTGTA 
TCCATGATTG ACCCACCCCG GGCCACCGTC CCTGATGCTG TGCTCAAGTG TCGCACCGCA 
GGCATCCGGG TGATCATGGT AACGGGTGAC CACCCCATCA CCGCCAAGGC CATTGCAGCC 
AGTGTGGGCA TCATCTCGGA AGGCAGCGAG ACAGTGGAGG ACATCGCTGC CCGCCTCCGT 
GTGCCCGTAG ACCAGGTTAA TCGCAAGGAT GCCCGTGCCT GTGTGATCAA TGGCATGCAG 
CTGAAGGACA TGGACCCATC GGAACTGGTC GAGGCCCTGC GCACCCACCC CGAGATGGTG 
TTTGCGCGCA CCAGCCCCCA GCAGAAGCTG GTGATCGTGG AGAGCTGCCA GCGGCTGGGT 
GCGATTGTGG CCGTCACGGG GGATGGTGTG AATGACTCCC CAGCTCTGAA GAAGGCAGAC 
ATCGGAGTAG CCATGGGCAT CGCTGGCTCA GATGCTGCCA AAAATGCAGC TGACATGATC 
CTGCTGGATG ACAACTTTGC CTCCATTGTG ACAGGCGTGG AGCAGGGTCG ACTGATCTTC 
GACAACCTGA AGAAGTCTAT TGCCTACACA TTGACCAAGA ACATCCCAGA GCTGACACCC 
TACCTCATCT ACATCACCGT CAGCGTGCCC CTGCCCCTCG GGTGCATCAC CATCCTCTTC 
ATCGAACTCT GCACTGACAT TTTCCCATCT GTGTCCCTGG CATATGAAAA GGCCGAGAGT 
GACATCATGC ACCTGCGTCC ACGCAACCCA AAGCGTGACA GATTGGTCAA CGAGCCCCTG 
GCTGCCTACT CCTACTTCCA GATTGGTGCC ATTCAGTCCT TTGCTGGCTT CACTGACTAC 
TTCACGGCAA TGGCCCAGGA GGGCTGGTTC CCACTGCTGT GCGTGGGGCT GCGGGCGCAG 
TGGGAGGACC ACCACCTACA AGATCTGCAG GACAGCTACG GCCAGGAGTG GACATTCGGG 
CAGCGCCTGT ACCAGCAGTA CACCTGCTAC ACCGTGTTCT TCATCAGCAT TGAGGTGTGC 
CAGATCGCCG ATGTCCTCAT CCGCAAGACG CGCCGTCTCT CTGCCTTCCA GCAAGGCTTC 
TTCAGGAATA AGATCCTGGT GATCGCCATC GTGTTCCAGG TCTGCATCGG CTGCTTCCTG 
TGCTACTGCC CCGGCATGCC CAACATCTTC AACTTCATGC CCATTCGGTT CCAGTGGTGG 
CTGGTCCCCC TGCCCTACGG CATCCTCATC TTCGTCTATG ATGAGATCCG GAAGCTTGGA 
GTTCGCTGTT GCCCAGGGAG CTGGTGGGAC CAGGAACTCT ACTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A cumulative effect involving malfunction of the PTH1R and ATP4A genes explains a familial gastric neuroendocrine tumor with hypothyroidism and arthritis.
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association20(6)998-1003(2017 Nov)
Calvete O,Herraiz M,Reyes J,Pati?o A,Benitez J

ATP4A autoimmunity in pediatric patients with type 1 diabetes and its relationship to blood count, iron metabolism, and vitamin B12.
Pediatric diabetes(2017 Apr)
Chobot A,Rusak E,Wenzlau J,Davidson H,Adamczyk P,Krzywicka A,Mazur B,Pola?ska J,Rewers M

A knockin mouse model for human ATP4aR703C mutation identified in familial gastric neuroendocrine tumors recapitulates the premalignant condition of the human disease and suggests new therapeutic strategies.
Disease models & mechanisms9(9)975-84(2016 09)
Calvete O,Varro A,Pritchard DM,Barroso A,Oteo M,Morcillo M?,Vargiu P,Dodd S,Garcia M,Reyes J,Ortega S,Benitez J

ECL-cell carcinoids and carcinoma in patients homozygous for an inactivating mutation in the gastric H(+) K(+) ATPase alpha subunit.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica124(7)561-6(2016 Jul)
Fossmark R,Calvete O,Mj?nes P,Benitez J,Waldum HL

Exome sequencing identifies ATP4A gene as responsible of an atypical familial type I gastric neuroendocrine tumour.
Human molecular genetics24(10)2914-22(2015 May)
Calvete O,Reyes J,Zu?iga S,Paumard-Hern?ndez B,Fern?ndez V,Bujanda L,Rodriguez-Pinilla MS,Palacios J,Heine-Su?er D,Banka S,Newman WG,Ca?amero M,Pritchard DM,Ben?tez J

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ATP4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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