OAZ1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	OAZ1
Entrez Gene ID	4946
Full Name	ornithine decarboxylase antizyme 1
Synonyms	AZ1,AZI,OAZ
General protein information	Preferred Names ornithine decarboxylase antizyme 1 Names ornithine decarboxylase antizyme 1 ODC-Az antizyme 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014].
Disorder MIM:	601579

mRNA and Protein(s)

mRNA	Protein	Name
NM_004152.3	NP_004143.1	ornithine decarboxylase antizyme 1 isoform 1
NM_001301020.1	NP_001287949.1	ornithine decarboxylase antizyme 1 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	OAZ1	NP_001120840.1
	OAZ1	NP_001128372.1
Canis lupus familiaris (dog)	OAZ1	NP_001120706.1
Rattus norvegicus (Norway rat)	Oaz1	NP_620781.1
Bos taurus (cattle)	OAZ1	NP_001120715.1
Homo sapiens (human)	OAZ1	NP_004143.1
Mus musculus (house mouse)	Oaz1	NP_032779.2
Gallus gallus (chicken)	OAZ1	NP_990247.1
Danio rerio (zebrafish)	oaz1a	NP_919240.2
Xenopus tropicalis (tropical clawed frog)	oaz1	NP_988880.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gand?a J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morat? X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Pr?ulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I
Molecular systems biology13(3)918(2017 03)

Structural basis of antizyme-mediated regulation of polyamine homeostasis.
Wu HY, Chen SF, Hsieh JY, Chou F, Wang YH, Lin WT, Lee PY, Yu YJ, Lin LY, Lin TS, Lin CL, Liu GY, Tzeng SR, Hung HC, Chan NL
Proceedings of the National Academy of Sciences of the United States of America112(36)11229-34(2015 Sep)

Effects of ornithine decarboxylase antizyme 1 on the proliferation and differentiation of human oral cancer cells.
Wang X, Jiang L
International journal of molecular medicine34(6)1606-12(2014 Dec)

Mitochondrial localization of antizyme is determined by context-dependent alternative utilization of two AUG initiation codons.
Gandre S, Bercovich Z, Kahana C
Mitochondrion2(4)245-56(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show the the interplay between the enzyme ornithine decarboxylase (ODC) and two regulatory proteins: antizyme (Az) and inhibitor (AzIN).
Title: Structural basis of antizyme-mediated regulation of polyamine homeostasis.

Results show that ornithine decarboxylase antizyme 1 (OAZ1) simultaneously inhibits the proliferation and induces the differentiation of oral cancer cells.
Title: Effects of ornithine decarboxylase antizyme 1 on the proliferation and differentiation of human oral cancer cells.

the effects of AZ on cell growth are independent of polyamines.
Title: Antizyme (AZ) regulates intestinal cell growth independent of polyamines.

To further understand its functions in CML pathogenesis, OAZ1 was overexpressed, and PCR array analysis was used to monitor the expression of key genes commonly involved in leukemia development.
Title: Assay of OAZ1 mRNA levels in chronic myeloid leukemia combined with application of leukemia PCR array identified relevant gene changes affected by antizyme.

Gene expression studies have identified altered expression of ornithine decarboxylase antizyme 1 in suicide completers with a history of mood disorders.
Title: Effects of promoter methylation on increased expression of polyamine biosynthetic genes in suicide.

The following OAZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OAZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu100967	NM_004152.3 Latest version!	Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu100968	NM_001301020.1 Latest version!	Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OAZ1 ( NM_004152.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004143.1 Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu100967
Clone ID Related Accession (Same CDS sequence)	NM_004152.3
Accession Version	NM_004152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ornithine decarboxylase antizyme 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY050868.1 and BC100291.1. On Jul 10, 2014 this sequence version replaced NM_004152.2. Transcript Variant: This variant (1) encodes the longer isoform (1). CCDS Note: The expression of the protein represented in this CCDS ID is auto-regulated by polyamine-enhanced translational frameshifting. During translation, a +1 frameshift occurs following codon 68, just preceding a termination codon, thereby enabling translation of the remaining portion of the protein (aa 69-228) in a different reading frame, as experimentally shown for the conserved rat ortholog (PMID:7813017). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.540760.1, SRR1163655.641932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation, expression, longest protein ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AATAGCCACT GCTTCGCCAG AGAGAAGGAA 
GGGGATAAAC CCAGCGCCAC CATCCACGCC AGCCGCACCA TGCCGCTCCT AAGCCTGCAC 
AGCCGCGGCG GCAGCAGCAG TGAGAGTTCC AGGGTCTCCC TCCACTGCTG TAGTAACCCG 
GGTCCGGGGC CTCGGTGGTG CTCCGATGCC CCTCACCCAC CCCTGAAGAT CCCAGGTGGG 
CGAGGGAATA GTCAGAGGGA TCACAATCTT TCAGCTAACT TATTCTACTC CGATGATCGG 
CTGAATGTAA CAGAGGAACT AACGTCCAAC GACAAGACGA GGATTCTCAA CGTCCAGTCC 
AGGCTCACAG ACGCCAAACG CATTAACTGG CGAACAGTGC TGAGTGGCGG CAGCCTCTAC 
ATCGAGATCC CGGGCGGCGC GCTGCCCGAG GGGAGCAAGG ACAGCTTTGC AGTTCTCCTG 
GAGTTCGCTG AGGAGCAGCT GCGAGCCGAC CATGTCTTCA TTTGCTTCCA CAAGAACCGC 
GAGGACAGAG CCGCCTTGCT CCGAACCTTC AGCTTTTTGG GCTTTGAGAT TGTGAGACCG 
GGGCATCCCC TTGTCCCCAA GAGACCCGAC GCTTGCTTCA TGGCCTACAC GTTCGAGAGA 
GAGTCTTCGG GAGAGGAGGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004143.1
CDS	join(114..317,319..801)
Translation	MVKSSLQRILNSHCFAREKEGDKPSATIHASRTMPLLSLHSRGGSSSESSRVSLHCCSNPGPGPRWCSDAPHPPLKIPGGRGNSQRDHNLSANLFYSDDRLNVTEELTSNDKTRILNVQSRLTDAKRINWRTVLSGGSLYIEIPGGALPEGSKDSFAVLLEFAEEQLRADHVFICFHKNREDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERESSGEEEE

Target ORF information:

RefSeq Version	NM_004152.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004152.3

ORF Insert Sequence:

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AATAGCCACT GCTTCGCCAG AGAGAAGGAA 
GGGGATAAAC CCAGCGCCAC CATCCACGCC AGCCGCACCA TGCCGCTCCT AAGCCTGCAC 
AGCCGCGGCG GCAGCAGCAG TGAGAGTTCC AGGGTCTCCC TCCACTGCTG TAGTAACCCG 
GGTCCGGGGC CTCGGTGGTG CTCCGATGCC CCTCACCCAC CCCTGAAGAT CCCAGGTGGG 
CGAGGGAATA GTCAGAGGGA TCACAATCTT TCAGCTAACT TATTCTACTC CGATGATCGG 
CTGAATGTAA CAGAGGAACT AACGTCCAAC GACAAGACGA GGATTCTCAA CGTCCAGTCC 
AGGCTCACAG ACGCCAAACG CATTAACTGG CGAACAGTGC TGAGTGGCGG CAGCCTCTAC 
ATCGAGATCC CGGGCGGCGC GCTGCCCGAG GGGAGCAAGG ACAGCTTTGC AGTTCTCCTG 
GAGTTCGCTG AGGAGCAGCT GCGAGCCGAC CATGTCTTCA TTTGCTTCCA CAAGAACCGC 
GAGGACAGAG CCGCCTTGCT CCGAACCTTC AGCTTTTTGG GCTTTGAGAT TGTGAGACCG 
GGGCATCCCC TTGTCCCCAA GAGACCCGAC GCTTGCTTCA TGGCCTACAC GTTCGAGAGA 
GAGTCTTCGG GAGAGGAGGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OAZ1 ( NM_001301020.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287949.1 Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100968
Clone ID Related Accession (Same CDS sequence)	NM_001301020.1
Accession Version	NM_001301020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ornithine decarboxylase antizyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY050868.1, BM423450.1 and BC100291.1. Transcript Variant: This variant (2) uses an alternate, in-frame acceptor splice site at the second exon compared to variant 1. The resulting isoform (2) lacks 2 internal amino acids compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.301072.1, SRR1163658.163636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AATAGCCACT GCTTCGCCAG AGAGAAGGAA 
GGGGATAAAC CCAGCGCCAC CATCCACGCC AGCCGCACCA TGCCGCTCCT AAGCCTGCAC 
AGCCGCGGCG GCAGCAGCAG TGAGAGGGTC TCCCTCCACT GCTGTAGTAA CCCGGGTCCG 
GGGCCTCGGT GGTGCTCCGA TGCCCCTCAC CCACCCCTGA AGATCCCAGG TGGGCGAGGG 
AATAGTCAGA GGGATCACAA TCTTTCAGCT AACTTATTCT ACTCCGATGA TCGGCTGAAT 
GTAACAGAGG AACTAACGTC CAACGACAAG ACGAGGATTC TCAACGTCCA GTCCAGGCTC 
ACAGACGCCA AACGCATTAA CTGGCGAACA GTGCTGAGTG GCGGCAGCCT CTACATCGAG 
ATCCCGGGCG GCGCGCTGCC CGAGGGGAGC AAGGACAGCT TTGCAGTTCT CCTGGAGTTC 
GCTGAGGAGC AGCTGCGAGC CGACCATGTC TTCATTTGCT TCCACAAGAA CCGCGAGGAC 
AGAGCCGCCT TGCTCCGAAC CTTCAGCTTT TTGGGCTTTG AGATTGTGAG ACCGGGGCAT 
CCCCTTGTCC CCAAGAGACC CGACGCTTGC TTCATGGCCT ACACGTTCGA GAGAGAGTCT 
TCGGGAGAGG AGGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287949.1
CDS	join(114..311,313..795)
Translation	MVKSSLQRILNSHCFAREKEGDKPSATIHASRTMPLLSLHSRGGSSSERVSLHCCSNPGPGPRWCSDAPHPPLKIPGGRGNSQRDHNLSANLFYSDDRLNVTEELTSNDKTRILNVQSRLTDAKRINWRTVLSGGSLYIEIPGGALPEGSKDSFAVLLEFAEEQLRADHVFICFHKNREDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERESSGEEEE

Target ORF information:

RefSeq Version	NM_001301020.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ornithine decarboxylase antizyme 1 (OAZ1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301020.1

ORF Insert Sequence:

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AATAGCCACT GCTTCGCCAG AGAGAAGGAA 
GGGGATAAAC CCAGCGCCAC CATCCACGCC AGCCGCACCA TGCCGCTCCT AAGCCTGCAC 
AGCCGCGGCG GCAGCAGCAG TGAGAGGGTC TCCCTCCACT GCTGTAGTAA CCCGGGTCCG 
GGGCCTCGGT GGTGCTCCGA TGCCCCTCAC CCACCCCTGA AGATCCCAGG TGGGCGAGGG 
AATAGTCAGA GGGATCACAA TCTTTCAGCT AACTTATTCT ACTCCGATGA TCGGCTGAAT 
GTAACAGAGG AACTAACGTC CAACGACAAG ACGAGGATTC TCAACGTCCA GTCCAGGCTC 
ACAGACGCCA AACGCATTAA CTGGCGAACA GTGCTGAGTG GCGGCAGCCT CTACATCGAG 
ATCCCGGGCG GCGCGCTGCC CGAGGGGAGC AAGGACAGCT TTGCAGTTCT CCTGGAGTTC 
GCTGAGGAGC AGCTGCGAGC CGACCATGTC TTCATTTGCT TCCACAAGAA CCGCGAGGAC 
AGAGCCGCCT TGCTCCGAAC CTTCAGCTTT TTGGGCTTTG AGATTGTGAG ACCGGGGCAT 
CCCCTTGTCC CCAAGAGACC CGACGCTTGC TTCATGGCCT ACACGTTCGA GAGAGAGTCT 
TCGGGAGAGG AGGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Molecular systems biology13(3)918(2017 03)
Sokolina K,Kittanakom S,Snider J,Kotlyar M,Maurice P,Gand?a J,Benleulmi-Chaachoua A,Tadagaki K,Oishi A,Wong V,Malty RH,Deineko V,Aoki H,Amin S,Yao Z,Morat? X,Otasek D,Kobayashi H,Menendez J,Auerbach D,Angers S,Pr?ulj N,Bouvier M,Babu M,Ciruela F,Jockers R,Jurisica I,Stagljar I

Structural basis of antizyme-mediated regulation of polyamine homeostasis.
Proceedings of the National Academy of Sciences of the United States of America112(36)11229-34(2015 Sep)
Wu HY,Chen SF,Hsieh JY,Chou F,Wang YH,Lin WT,Lee PY,Yu YJ,Lin LY,Lin TS,Lin CL,Liu GY,Tzeng SR,Hung HC,Chan NL

Effects of ornithine decarboxylase antizyme 1 on the proliferation and differentiation of human oral cancer cells.
International journal of molecular medicine34(6)1606-12(2014 Dec)
Wang X,Jiang L

Mitochondrial localization of antizyme is determined by context-dependent alternative utilization of two AUG initiation codons.
Mitochondrion2(4)245-56(2003 Mar)
Gandre S,Bercovich Z,Kahana C

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OAZ1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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