TBC1D25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBC1D25
Entrez Gene ID	4943
Full Name	TBC1 domain family member 25
Synonyms	MG81,OATL1
General protein information	Preferred Names TBC1 domain family member 25 Names TBC1 domain family member 25 5SN3 snoRNA ornithine aminotransferase-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.23
Summary	This gene encodes a protein with a TBC domain and functions as a Rab GTPase activating protein. The encoded protein is involved in the fusion of autophagosomes with endosomes and lysosomes. This gene was previously known as ornithine aminotransferase-like 1, but has no similarity to ornithine aminotransferase. [provided by RefSeq, Jan 2017].
Disorder MIM:	311240

mRNA and Protein(s)

mRNA	Protein	Name
NM_001348264.1	NP_001335193.1	TBC1 domain family member 25 isoform d
NM_001348262.1	NP_001335191.1	TBC1 domain family member 25 isoform a
NM_001348263.1	NP_001335192.1	TBC1 domain family member 25 isoform b
NM_002536.4	NP_002527.1	TBC1 domain family member 25 isoform c
NM_002536.3	NP_002527.1	TBC1 domain family member 25 isoform c
NM_001348265.1	NP_001335194.1	TBC1 domain family member 25 isoform d

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TBC1D25	XP_005641377.1
Rattus norvegicus (Norway rat)	Tbc1d25	NP_001100425.1
Danio rerio (zebrafish)	tbc1d25	NP_001121708.1
Xenopus tropicalis (tropical clawed frog)	LOC100495342	XP_002943822.2
Pan troglodytes (chimpanzee)	TBC1D25	XP_001139235.2
Mus musculus (house mouse)	Tbc1d25	NP_001159909.1
	TBC1D25	XP_001098742.1
Homo sapiens (human)	TBC1D25	NP_002527.1
Bos taurus (cattle)	TBC1D25	NP_001192742.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A systematic High-Content Screening microscopy approach reveals key roles for Rab33b, OATL1 and Myo6 in nanoparticle trafficking in HeLa cells.
Panarella A, Bexiga MG, Galea G, O' Neill ED, Salvati A, Dawson KA, Simpson JC
Scientific reports628865(2016 Jul)

Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1.
Hirano S, Uemura T, Annoh H, Fujita N, Waguri S, Itoh T, Fukuda M
Autophagy12(2)312-26(2016)

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.
Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I
Molecular and cellular biology32(9)1733-44(2012 May)

OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation.
Itoh T, Kanno E, Uemura T, Waguri S, Fukuda M
The Journal of cell biology192(5)839-53(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that each of the 4 genes was associated with at least one main outcome: anxiety (SAT1, SMS), mood disorders (SAT1, SMOX), and suicide attempts (SAT1, OATL1).
Title: Association of polyaminergic loci with anxiety, mood disorders, and attempted suicide.

Our study showed that adenovirus-mediated antisense ODC and expression inhibits tumor cell growth through blocking the polyamine synthesis pathway.
Title: Adenovirus-mediated expression of both antisense ornithine decarboxylase and S-adenosylmethionine decarboxylase inhibits lung cancer cell growth.

The following TBC1D25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03933	NM_002536.4 Latest version!	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu102286	NM_001348264.1 Latest version!	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu102286	NM_001348265.1 Latest version!	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu102355	NM_001348263.1 Latest version!	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102358	NM_001348262.1 Latest version!	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu03933	NM_002536.3	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBC1D25 ( NM_002536.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002527.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03933
Clone ID Related Accession (Same CDS sequence)	NM_002536.4 , NM_002536.3
Accession Version	NM_002536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154299.1, BC101817.1, AF196969.3, AC115618.3 and BU630180.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002536.3. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.156985.1, SRR1803615.174231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376771.9/ ENSP00000365962.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGAAATGTG AGAGCTTCTT GCCGCCAGAG TTCCGCTCTT TTGCTGTAGA TCCCCAGATC 
ACCTCGCTCG ACGTGTTGCA GCACATCCTC ATCCGAGCCT TTGATTTGAG TGGGAAGAAG 
AACTTTGGCA TCAGCTACCT GGGCCGGGAC CGGCTAGGAC AGGAAGTTTA CCTCTCACTT 
CTATCTGACT GGGACCTCAG CACAGCCTTT GCCACTGCCT CCAAACCTTA CCTGCAATTG 
CGTGTAGATA TTCGGCCCTC GGAGGACAGC CCATTGCTAG AAGACTGGGA CATAATCAGC 
CCCAAAGATG TCATTGGCTC CGACGTGTTG CTGGCTGAGA AACGGTCATC ACTGACGACT 
GCCGCCCTGC CCTTTACACA GTCCATCCTC ACTCAGGTGG GCCGTACCTT GTCTAAGGTC 
CAACAAGTGC TGAGCTGGTC GTATGGGGAA GATGTCAAGC CCTTCAAGCC ACCCCTGAGC 
GATGCTGAGT TTCACACGTA CCTGAACCAC GAGGGCCAGC TCTCCCGACC CGAGGAGTTG 
CGCCTGCGGA TCTATCATGG CGGTGTGGAG CCCTCGCTGC GAAAGGTGGT GTGGCGGTAC 
CTGCTGAACG TGTATCCAGA TGGACTGACA GGCCGAGAGC GGATGGACTA CATGAAACGC 
AAGAGCCGCG AGTATGAGCA GCTCAAGAGC GAGTGGGCCC AGCGAGCGAA CCCTGAGGAC 
CTGGAATTCA TCCGCAGCAC GGTCCTCAAG GATGTACTGC GCACTGACCG GGCCCACCCC 
TACTATGCGG GGCCTGAGGA TGGCCCACAT CTACGGGCGC TGCACGACCT GCTCACCACC 
TATGCCGTTA CCCACCCACA GGTGTCCTAC TGCCAGGGCA TGAGTGACCT TGCCTCACCC 
ATCCTCGCTG TCATGGACCA TGAGGGCCAT GCCTTTGTTT GCTTTTGTGG CATCATGAAA 
CGCCTGGCCG CCAACTTCCA CCCTGACGGC CGCGCCATGG CCACCAAGTT TGCACACTTG 
AAGCTGTTGC TGCGACACGC TGACCCTGAC TTTTATCAAT ACCTGCAAGA GGCAGGCGCT 
GATGACCTCT TCTTCTGTTA CCGCTGGCTG CTGCTGGAAC TCAAGCGTGA GTTCGCCTTC 
GACGATGCCC TCCGCATGCT TGAGGTCACT TGGAGTTCGC TGCCCCCTGA TCCTCCTGAA 
CATGAGGTAG AGCTGGTTGG ACCCCCCAGC CAAGTGGCAG ACGCTGGTTT TGGTGGCCAC 
AGGGGGTGGC CCGTGCGACA GAGGCACATG CTGAGGCCTG CTGGTGGAGG AGGTAGTACC 
TTTGAAGATG CTGTTGACCA CCTGGCCACA GCCAGTCAGG GGCCTGGTGG TGGGGGGCGT 
CTCCTGAGAC AGGCCAGCTT GGATGGCCTC CAGCAACTCA GGGATAACAT GGGCTCCAGG 
AGGGACCCTC TGGTCCAGCT GCCCCACCCA GCTGCCCTTA TCAGCTCCAA GTCCCTCTCT 
GAGCCTTTAT TGAACTCCCC AGACCCACTG CTCTCCTCCT TTTCCCACCC TGATTCCCCA 
TCTTCCTCAT CTCCACCATC CACCCAGGAG GCCTCTCCCA CTGGTGATAT GGCTGTAGGA 
TCCCCCTTGA TGCAAGAGGT AGGCTCCCCG AAAGACCCTG GAAAGTCCCT GCCACCTGTA 
CCACCAATGG GCCTGCCCCC ACCCCAGGAG TTTGGCCGGG GGAACCCATT CATGCTGTTC 
CTCTGCCTGG CCATCCTGCT GGAGCACCGC GACCACATCA TGCGCAATGG GCTGGATTAT 
AATGAGCTGG CCATGCACTT TGACCGCCTT GTGCGAAAAC ACCACCTGGG GCGCGTCCTG 
CGCCGGGCTA GGGCTCTCTT TGCTGATTAC CTGCAGTCAG AGGTGTGGGA CTCAGAGGAG 
GGGGCTGAGG CCACAGCCGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002527.1
CDS	85..2151
Translation	MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKKCESFLPPEFRSFAVDPQITSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_002536.4
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002536.4

ORF Insert Sequence:

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGAAATGTG AGAGCTTCTT GCCGCCAGAG TTCCGCTCTT TTGCTGTAGA TCCCCAGATC 
ACCTCGCTCG ACGTGTTGCA GCACATCCTC ATCCGAGCCT TTGATTTGAG TGGGAAGAAG 
AACTTTGGCA TCAGCTACCT GGGCCGGGAC CGGCTAGGAC AGGAAGTTTA CCTCTCACTT 
CTATCTGACT GGGACCTCAG CACAGCCTTT GCCACTGCCT CCAAACCTTA CCTGCAATTG 
CGTGTAGATA TTCGGCCCTC GGAGGACAGC CCATTGCTAG AAGACTGGGA CATAATCAGC 
CCCAAAGATG TCATTGGCTC CGACGTGTTG CTGGCTGAGA AACGGTCATC ACTGACGACT 
GCCGCCCTGC CCTTTACACA GTCCATCCTC ACTCAGGTGG GCCGTACCTT GTCTAAGGTC 
CAACAAGTGC TGAGCTGGTC GTATGGGGAA GATGTCAAGC CCTTCAAGCC ACCCCTGAGC 
GATGCTGAGT TTCACACGTA CCTGAACCAC GAGGGCCAGC TCTCCCGACC CGAGGAGTTG 
CGCCTGCGGA TCTATCATGG CGGTGTGGAG CCCTCGCTGC GAAAGGTGGT GTGGCGGTAC 
CTGCTGAACG TGTATCCAGA TGGACTGACA GGCCGAGAGC GGATGGACTA CATGAAACGC 
AAGAGCCGCG AGTATGAGCA GCTCAAGAGC GAGTGGGCCC AGCGAGCGAA CCCTGAGGAC 
CTGGAATTCA TCCGCAGCAC GGTCCTCAAG GATGTACTGC GCACTGACCG GGCCCACCCC 
TACTATGCGG GGCCTGAGGA TGGCCCACAT CTACGGGCGC TGCACGACCT GCTCACCACC 
TATGCCGTTA CCCACCCACA GGTGTCCTAC TGCCAGGGCA TGAGTGACCT TGCCTCACCC 
ATCCTCGCTG TCATGGACCA TGAGGGCCAT GCCTTTGTTT GCTTTTGTGG CATCATGAAA 
CGCCTGGCCG CCAACTTCCA CCCTGACGGC CGCGCCATGG CCACCAAGTT TGCACACTTG 
AAGCTGTTGC TGCGACACGC TGACCCTGAC TTTTATCAAT ACCTGCAAGA GGCAGGCGCT 
GATGACCTCT TCTTCTGTTA CCGCTGGCTG CTGCTGGAAC TCAAGCGTGA GTTCGCCTTC 
GACGATGCCC TCCGCATGCT TGAGGTCACT TGGAGTTCGC TGCCCCCTGA TCCTCCTGAA 
CATGAGGTAG AGCTGGTTGG ACCCCCCAGC CAAGTGGCAG ACGCTGGTTT TGGTGGCCAC 
AGGGGGTGGC CCGTGCGACA GAGGCACATG CTGAGGCCTG CTGGTGGAGG AGGTAGTACC 
TTTGAAGATG CTGTTGACCA CCTGGCCACA GCCAGTCAGG GGCCTGGTGG TGGGGGGCGT 
CTCCTGAGAC AGGCCAGCTT GGATGGCCTC CAGCAACTCA GGGATAACAT GGGCTCCAGG 
AGGGACCCTC TGGTCCAGCT GCCCCACCCA GCTGCCCTTA TCAGCTCCAA GTCCCTCTCT 
GAGCCTTTAT TGAACTCCCC AGACCCACTG CTCTCCTCCT TTTCCCACCC TGATTCCCCA 
TCTTCCTCAT CTCCACCATC CACCCAGGAG GCCTCTCCCA CTGGTGATAT GGCTGTAGGA 
TCCCCCTTGA TGCAAGAGGT AGGCTCCCCG AAAGACCCTG GAAAGTCCCT GCCACCTGTA 
CCACCAATGG GCCTGCCCCC ACCCCAGGAG TTTGGCCGGG GGAACCCATT CATGCTGTTC 
CTCTGCCTGG CCATCCTGCT GGAGCACCGC GACCACATCA TGCGCAATGG GCTGGATTAT 
AATGAGCTGG CCATGCACTT TGACCGCCTT GTGCGAAAAC ACCACCTGGG GCGCGTCCTG 
CGCCGGGCTA GGGCTCTCTT TGCTGATTAC CTGCAGTCAG AGGTGTGGGA CTCAGAGGAG 
GGGGCTGAGG CCACAGCCGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D25 ( NM_002536.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002527.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03933
Clone ID Related Accession (Same CDS sequence)	NM_002536.4 , NM_002536.3
Accession Version	NM_002536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154299.1, BC101817.1, AF196969.3, AC115618.3 and BU630180.1. This sequence is a reference standard in the RefSeqGene project. On Jan 24, 2017 this sequence version replaced NM_002536.2. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.156985.1, SRR1803615.174231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGAAATGTG AGAGCTTCTT GCCGCCAGAG TTCCGCTCTT TTGCTGTAGA TCCCCAGATC 
ACCTCGCTCG ACGTGTTGCA GCACATCCTC ATCCGAGCCT TTGATTTGAG TGGGAAGAAG 
AACTTTGGCA TCAGCTACCT GGGCCGGGAC CGGCTAGGAC AGGAAGTTTA CCTCTCACTT 
CTATCTGACT GGGACCTCAG CACAGCCTTT GCCACTGCCT CCAAACCTTA CCTGCAATTG 
CGTGTAGATA TTCGGCCCTC GGAGGACAGC CCATTGCTAG AAGACTGGGA CATAATCAGC 
CCCAAAGATG TCATTGGCTC CGACGTGTTG CTGGCTGAGA AACGGTCATC ACTGACGACT 
GCCGCCCTGC CCTTTACACA GTCCATCCTC ACTCAGGTGG GCCGTACCTT GTCTAAGGTC 
CAACAAGTGC TGAGCTGGTC GTATGGGGAA GATGTCAAGC CCTTCAAGCC ACCCCTGAGC 
GATGCTGAGT TTCACACGTA CCTGAACCAC GAGGGCCAGC TCTCCCGACC CGAGGAGTTG 
CGCCTGCGGA TCTATCATGG CGGTGTGGAG CCCTCGCTGC GAAAGGTGGT GTGGCGGTAC 
CTGCTGAACG TGTATCCAGA TGGACTGACA GGCCGAGAGC GGATGGACTA CATGAAACGC 
AAGAGCCGCG AGTATGAGCA GCTCAAGAGC GAGTGGGCCC AGCGAGCGAA CCCTGAGGAC 
CTGGAATTCA TCCGCAGCAC GGTCCTCAAG GATGTACTGC GCACTGACCG GGCCCACCCC 
TACTATGCGG GGCCTGAGGA TGGCCCACAT CTACGGGCGC TGCACGACCT GCTCACCACC 
TATGCCGTTA CCCACCCACA GGTGTCCTAC TGCCAGGGCA TGAGTGACCT TGCCTCACCC 
ATCCTCGCTG TCATGGACCA TGAGGGCCAT GCCTTTGTTT GCTTTTGTGG CATCATGAAA 
CGCCTGGCCG CCAACTTCCA CCCTGACGGC CGCGCCATGG CCACCAAGTT TGCACACTTG 
AAGCTGTTGC TGCGACACGC TGACCCTGAC TTTTATCAAT ACCTGCAAGA GGCAGGCGCT 
GATGACCTCT TCTTCTGTTA CCGCTGGCTG CTGCTGGAAC TCAAGCGTGA GTTCGCCTTC 
GACGATGCCC TCCGCATGCT TGAGGTCACT TGGAGTTCGC TGCCCCCTGA TCCTCCTGAA 
CATGAGGTAG AGCTGGTTGG ACCCCCCAGC CAAGTGGCAG ACGCTGGTTT TGGTGGCCAC 
AGGGGGTGGC CCGTGCGACA GAGGCACATG CTGAGGCCTG CTGGTGGAGG AGGTAGTACC 
TTTGAAGATG CTGTTGACCA CCTGGCCACA GCCAGTCAGG GGCCTGGTGG TGGGGGGCGT 
CTCCTGAGAC AGGCCAGCTT GGATGGCCTC CAGCAACTCA GGGATAACAT GGGCTCCAGG 
AGGGACCCTC TGGTCCAGCT GCCCCACCCA GCTGCCCTTA TCAGCTCCAA GTCCCTCTCT 
GAGCCTTTAT TGAACTCCCC AGACCCACTG CTCTCCTCCT TTTCCCACCC TGATTCCCCA 
TCTTCCTCAT CTCCACCATC CACCCAGGAG GCCTCTCCCA CTGGTGATAT GGCTGTAGGA 
TCCCCCTTGA TGCAAGAGGT AGGCTCCCCG AAAGACCCTG GAAAGTCCCT GCCACCTGTA 
CCACCAATGG GCCTGCCCCC ACCCCAGGAG TTTGGCCGGG GGAACCCATT CATGCTGTTC 
CTCTGCCTGG CCATCCTGCT GGAGCACCGC GACCACATCA TGCGCAATGG GCTGGATTAT 
AATGAGCTGG CCATGCACTT TGACCGCCTT GTGCGAAAAC ACCACCTGGG GCGCGTCCTG 
CGCCGGGCTA GGGCTCTCTT TGCTGATTAC CTGCAGTCAG AGGTGTGGGA CTCAGAGGAG 
GGGGCTGAGG CCACAGCCGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002527.1
CDS	180..2246
Translation	MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKKCESFLPPEFRSFAVDPQITSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_002536.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002536.3

ORF Insert Sequence:

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGAAATGTG AGAGCTTCTT GCCGCCAGAG TTCCGCTCTT TTGCTGTAGA TCCCCAGATC 
ACCTCGCTCG ACGTGTTGCA GCACATCCTC ATCCGAGCCT TTGATTTGAG TGGGAAGAAG 
AACTTTGGCA TCAGCTACCT GGGCCGGGAC CGGCTAGGAC AGGAAGTTTA CCTCTCACTT 
CTATCTGACT GGGACCTCAG CACAGCCTTT GCCACTGCCT CCAAACCTTA CCTGCAATTG 
CGTGTAGATA TTCGGCCCTC GGAGGACAGC CCATTGCTAG AAGACTGGGA CATAATCAGC 
CCCAAAGATG TCATTGGCTC CGACGTGTTG CTGGCTGAGA AACGGTCATC ACTGACGACT 
GCCGCCCTGC CCTTTACACA GTCCATCCTC ACTCAGGTGG GCCGTACCTT GTCTAAGGTC 
CAACAAGTGC TGAGCTGGTC GTATGGGGAA GATGTCAAGC CCTTCAAGCC ACCCCTGAGC 
GATGCTGAGT TTCACACGTA CCTGAACCAC GAGGGCCAGC TCTCCCGACC CGAGGAGTTG 
CGCCTGCGGA TCTATCATGG CGGTGTGGAG CCCTCGCTGC GAAAGGTGGT GTGGCGGTAC 
CTGCTGAACG TGTATCCAGA TGGACTGACA GGCCGAGAGC GGATGGACTA CATGAAACGC 
AAGAGCCGCG AGTATGAGCA GCTCAAGAGC GAGTGGGCCC AGCGAGCGAA CCCTGAGGAC 
CTGGAATTCA TCCGCAGCAC GGTCCTCAAG GATGTACTGC GCACTGACCG GGCCCACCCC 
TACTATGCGG GGCCTGAGGA TGGCCCACAT CTACGGGCGC TGCACGACCT GCTCACCACC 
TATGCCGTTA CCCACCCACA GGTGTCCTAC TGCCAGGGCA TGAGTGACCT TGCCTCACCC 
ATCCTCGCTG TCATGGACCA TGAGGGCCAT GCCTTTGTTT GCTTTTGTGG CATCATGAAA 
CGCCTGGCCG CCAACTTCCA CCCTGACGGC CGCGCCATGG CCACCAAGTT TGCACACTTG 
AAGCTGTTGC TGCGACACGC TGACCCTGAC TTTTATCAAT ACCTGCAAGA GGCAGGCGCT 
GATGACCTCT TCTTCTGTTA CCGCTGGCTG CTGCTGGAAC TCAAGCGTGA GTTCGCCTTC 
GACGATGCCC TCCGCATGCT TGAGGTCACT TGGAGTTCGC TGCCCCCTGA TCCTCCTGAA 
CATGAGGTAG AGCTGGTTGG ACCCCCCAGC CAAGTGGCAG ACGCTGGTTT TGGTGGCCAC 
AGGGGGTGGC CCGTGCGACA GAGGCACATG CTGAGGCCTG CTGGTGGAGG AGGTAGTACC 
TTTGAAGATG CTGTTGACCA CCTGGCCACA GCCAGTCAGG GGCCTGGTGG TGGGGGGCGT 
CTCCTGAGAC AGGCCAGCTT GGATGGCCTC CAGCAACTCA GGGATAACAT GGGCTCCAGG 
AGGGACCCTC TGGTCCAGCT GCCCCACCCA GCTGCCCTTA TCAGCTCCAA GTCCCTCTCT 
GAGCCTTTAT TGAACTCCCC AGACCCACTG CTCTCCTCCT TTTCCCACCC TGATTCCCCA 
TCTTCCTCAT CTCCACCATC CACCCAGGAG GCCTCTCCCA CTGGTGATAT GGCTGTAGGA 
TCCCCCTTGA TGCAAGAGGT AGGCTCCCCG AAAGACCCTG GAAAGTCCCT GCCACCTGTA 
CCACCAATGG GCCTGCCCCC ACCCCAGGAG TTTGGCCGGG GGAACCCATT CATGCTGTTC 
CTCTGCCTGG CCATCCTGCT GGAGCACCGC GACCACATCA TGCGCAATGG GCTGGATTAT 
AATGAGCTGG CCATGCACTT TGACCGCCTT GTGCGAAAAC ACCACCTGGG GCGCGTCCTG 
CGCCGGGCTA GGGCTCTCTT TGCTGATTAC CTGCAGTCAG AGGTGTGGGA CTCAGAGGAG 
GGGGCTGAGG CCACAGCCGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D25 ( NM_001348264.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335193.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu102286
Clone ID Related Accession (Same CDS sequence)	NM_001348264.1 , NM_001348265.1
Accession Version	NM_001348264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF196969.3 and AC115618.3. Transcript Variant: This variant (4) uses an alternate splice junction compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. Variants 4 and 5 both encode the same isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.131616.1, SRR1660809.222675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGGA CTGGCCAGTG GGCCACCGAC CTCTACAGTG GCTATCTGGC CTTTGTTTGG 
AAGAAGAACT TTGGCATCAG CTACCTGGGC CGGGACCGGC TAGGACAGGA AGTTTACCTC 
TCACTTCTAT CTGACTGGGA CCTCAGCACA GCCTTTGCCA CTGCCTCCAA ACCTTACCTG 
CAATTGCGTG TAGATATTCG GCCCTCGGAG GACAGCCCAT TGCTAGAAGA CTGGGACATA 
ATCAGCCCCA AAGATGTCAT TGGCTCCGAC GTGTTGCTGG CTGAGAAACG GTCATCACTG 
ACGACTGCCG CCCTGCCCTT TACACAGTCC ATCCTCACTC AGGTGGGCCG TACCTTGTCT 
AAGGTCCAAC AAGTGCTGAG CTGGTCGTAT GGGGAAGATG TCAAGCCCTT CAAGCCACCC 
CTGAGCGATG CTGAGTTTCA CACGTACCTG AACCACGAGG GCCAGCTCTC CCGACCCGAG 
GAGTTGCGCC TGCGGATCTA TCATGGCGGT GTGGAGCCCT CGCTGCGAAA GGTGGTGTGG 
CGGTACCTGC TGAACGTGTA TCCAGATGGA CTGACAGGCC GAGAGCGGAT GGACTACATG 
AAACGCAAGA GCCGCGAGTA TGAGCAGCTC AAGAGCGAGT GGGCCCAGCG AGCGAACCCT 
GAGGACCTGG AATTCATCCG CAGCACGGTC CTCAAGGATG TACTGCGCAC TGACCGGGCC 
CACCCCTACT ATGCGGGGCC TGAGGATGGC CCACATCTAC GGGCGCTGCA CGACCTGCTC 
ACCACCTATG CCGTTACCCA CCCACAGGTG TCCTACTGCC AGGGCATGAG TGACCTTGCC 
TCACCCATCC TCGCTGTCAT GGACCATGAG GGCCATGCCT TTGTTTGCTT TTGTGGCATC 
ATGAAACGCC TGGCCGCCAA CTTCCACCCT GACGGCCGCG CCATGGCCAC CAAGTTTGCA 
CACTTGAAGC TGTTGCTGCG ACACGCTGAC CCTGACTTTT ATCAATACCT GCAAGAGGCA 
GGCGCTGATG ACCTCTTCTT CTGTTACCGC TGGCTGCTGC TGGAACTCAA GCGTGAGTTC 
GCCTTCGACG ATGCCCTCCG CATGCTTGAG GTCACTTGGA GTTCGCTGCC CCCTGATCCT 
CCTGAACATG AGGTAGAGCT GGTTGGACCC CCCAGCCAAG TGGCAGACGC TGGTTTTGGT 
GGCCACAGGG GGTGGCCCGT GCGACAGAGG CACATGCTGA GGCCTGCTGG TGGAGGAGGT 
AGTACCTTTG AAGATGCTGT TGACCACCTG GCCACAGCCA GTCAGGGGCC TGGTGGTGGG 
GGGCGTCTCC TGAGACAGGC CAGCTTGGAT GGCCTCCAGC AACTCAGGGA TAACATGGGC 
TCCAGGAGGG ACCCTCTGGT CCAGCTGCCC CACCCAGCTG CCCTTATCAG CTCCAAGTCC 
CTCTCTGAGC CTTTATTGAA CTCCCCAGAC CCACTGCTCT CCTCCTTTTC CCACCCTGAT 
TCCCCATCTT CCTCATCTCC ACCATCCACC CAGGAGGCCT CTCCCACTGG TGATATGGCT 
GTAGGATCCC CCTTGATGCA AGAGGTAGGC TCCCCGAAAG ACCCTGGAAA GTCCCTGCCA 
CCTGTACCAC CAATGGGCCT GCCCCCACCC CAGGAGTTTG GCCGGGGGAA CCCATTCATG 
CTGTTCCTCT GCCTGGCCAT CCTGCTGGAG CACCGCGACC ACATCATGCG CAATGGGCTG 
GATTATAATG AGCTGGCCAT GCACTTTGAC CGCCTTGTGC GAAAACACCA CCTGGGGCGC 
GTCCTGCGCC GGGCTAGGGC TCTCTTTGCT GATTACCTGC AGTCAGAGGT GTGGGACTCA 
GAGGAGGGGG CTGAGGCCAC AGCCGCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335193.1
CDS	425..2317
Translation	MLGTGQWATDLYSGYLAFVWKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_001348264.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348264.1

ORF Insert Sequence:

ATGCTGGGGA CTGGCCAGTG GGCCACCGAC CTCTACAGTG GCTATCTGGC CTTTGTTTGG 
AAGAAGAACT TTGGCATCAG CTACCTGGGC CGGGACCGGC TAGGACAGGA AGTTTACCTC 
TCACTTCTAT CTGACTGGGA CCTCAGCACA GCCTTTGCCA CTGCCTCCAA ACCTTACCTG 
CAATTGCGTG TAGATATTCG GCCCTCGGAG GACAGCCCAT TGCTAGAAGA CTGGGACATA 
ATCAGCCCCA AAGATGTCAT TGGCTCCGAC GTGTTGCTGG CTGAGAAACG GTCATCACTG 
ACGACTGCCG CCCTGCCCTT TACACAGTCC ATCCTCACTC AGGTGGGCCG TACCTTGTCT 
AAGGTCCAAC AAGTGCTGAG CTGGTCGTAT GGGGAAGATG TCAAGCCCTT CAAGCCACCC 
CTGAGCGATG CTGAGTTTCA CACGTACCTG AACCACGAGG GCCAGCTCTC CCGACCCGAG 
GAGTTGCGCC TGCGGATCTA TCATGGCGGT GTGGAGCCCT CGCTGCGAAA GGTGGTGTGG 
CGGTACCTGC TGAACGTGTA TCCAGATGGA CTGACAGGCC GAGAGCGGAT GGACTACATG 
AAACGCAAGA GCCGCGAGTA TGAGCAGCTC AAGAGCGAGT GGGCCCAGCG AGCGAACCCT 
GAGGACCTGG AATTCATCCG CAGCACGGTC CTCAAGGATG TACTGCGCAC TGACCGGGCC 
CACCCCTACT ATGCGGGGCC TGAGGATGGC CCACATCTAC GGGCGCTGCA CGACCTGCTC 
ACCACCTATG CCGTTACCCA CCCACAGGTG TCCTACTGCC AGGGCATGAG TGACCTTGCC 
TCACCCATCC TCGCTGTCAT GGACCATGAG GGCCATGCCT TTGTTTGCTT TTGTGGCATC 
ATGAAACGCC TGGCCGCCAA CTTCCACCCT GACGGCCGCG CCATGGCCAC CAAGTTTGCA 
CACTTGAAGC TGTTGCTGCG ACACGCTGAC CCTGACTTTT ATCAATACCT GCAAGAGGCA 
GGCGCTGATG ACCTCTTCTT CTGTTACCGC TGGCTGCTGC TGGAACTCAA GCGTGAGTTC 
GCCTTCGACG ATGCCCTCCG CATGCTTGAG GTCACTTGGA GTTCGCTGCC CCCTGATCCT 
CCTGAACATG AGGTAGAGCT GGTTGGACCC CCCAGCCAAG TGGCAGACGC TGGTTTTGGT 
GGCCACAGGG GGTGGCCCGT GCGACAGAGG CACATGCTGA GGCCTGCTGG TGGAGGAGGT 
AGTACCTTTG AAGATGCTGT TGACCACCTG GCCACAGCCA GTCAGGGGCC TGGTGGTGGG 
GGGCGTCTCC TGAGACAGGC CAGCTTGGAT GGCCTCCAGC AACTCAGGGA TAACATGGGC 
TCCAGGAGGG ACCCTCTGGT CCAGCTGCCC CACCCAGCTG CCCTTATCAG CTCCAAGTCC 
CTCTCTGAGC CTTTATTGAA CTCCCCAGAC CCACTGCTCT CCTCCTTTTC CCACCCTGAT 
TCCCCATCTT CCTCATCTCC ACCATCCACC CAGGAGGCCT CTCCCACTGG TGATATGGCT 
GTAGGATCCC CCTTGATGCA AGAGGTAGGC TCCCCGAAAG ACCCTGGAAA GTCCCTGCCA 
CCTGTACCAC CAATGGGCCT GCCCCCACCC CAGGAGTTTG GCCGGGGGAA CCCATTCATG 
CTGTTCCTCT GCCTGGCCAT CCTGCTGGAG CACCGCGACC ACATCATGCG CAATGGGCTG 
GATTATAATG AGCTGGCCAT GCACTTTGAC CGCCTTGTGC GAAAACACCA CCTGGGGCGC 
GTCCTGCGCC GGGCTAGGGC TCTCTTTGCT GATTACCTGC AGTCAGAGGT GTGGGACTCA 
GAGGAGGGGG CTGAGGCCAC AGCCGCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D25 ( NM_001348265.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335194.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102286
Clone ID Related Accession (Same CDS sequence)	NM_001348264.1 , NM_001348265.1
Accession Version	NM_001348265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF196969.3 and AC115618.3. Transcript Variant: This variant (5) uses two alternate splice junctions compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. Variants 4 and 5 both encode the same isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.186868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGGA CTGGCCAGTG GGCCACCGAC CTCTACAGTG GCTATCTGGC CTTTGTTTGG 
AAGAAGAACT TTGGCATCAG CTACCTGGGC CGGGACCGGC TAGGACAGGA AGTTTACCTC 
TCACTTCTAT CTGACTGGGA CCTCAGCACA GCCTTTGCCA CTGCCTCCAA ACCTTACCTG 
CAATTGCGTG TAGATATTCG GCCCTCGGAG GACAGCCCAT TGCTAGAAGA CTGGGACATA 
ATCAGCCCCA AAGATGTCAT TGGCTCCGAC GTGTTGCTGG CTGAGAAACG GTCATCACTG 
ACGACTGCCG CCCTGCCCTT TACACAGTCC ATCCTCACTC AGGTGGGCCG TACCTTGTCT 
AAGGTCCAAC AAGTGCTGAG CTGGTCGTAT GGGGAAGATG TCAAGCCCTT CAAGCCACCC 
CTGAGCGATG CTGAGTTTCA CACGTACCTG AACCACGAGG GCCAGCTCTC CCGACCCGAG 
GAGTTGCGCC TGCGGATCTA TCATGGCGGT GTGGAGCCCT CGCTGCGAAA GGTGGTGTGG 
CGGTACCTGC TGAACGTGTA TCCAGATGGA CTGACAGGCC GAGAGCGGAT GGACTACATG 
AAACGCAAGA GCCGCGAGTA TGAGCAGCTC AAGAGCGAGT GGGCCCAGCG AGCGAACCCT 
GAGGACCTGG AATTCATCCG CAGCACGGTC CTCAAGGATG TACTGCGCAC TGACCGGGCC 
CACCCCTACT ATGCGGGGCC TGAGGATGGC CCACATCTAC GGGCGCTGCA CGACCTGCTC 
ACCACCTATG CCGTTACCCA CCCACAGGTG TCCTACTGCC AGGGCATGAG TGACCTTGCC 
TCACCCATCC TCGCTGTCAT GGACCATGAG GGCCATGCCT TTGTTTGCTT TTGTGGCATC 
ATGAAACGCC TGGCCGCCAA CTTCCACCCT GACGGCCGCG CCATGGCCAC CAAGTTTGCA 
CACTTGAAGC TGTTGCTGCG ACACGCTGAC CCTGACTTTT ATCAATACCT GCAAGAGGCA 
GGCGCTGATG ACCTCTTCTT CTGTTACCGC TGGCTGCTGC TGGAACTCAA GCGTGAGTTC 
GCCTTCGACG ATGCCCTCCG CATGCTTGAG GTCACTTGGA GTTCGCTGCC CCCTGATCCT 
CCTGAACATG AGGTAGAGCT GGTTGGACCC CCCAGCCAAG TGGCAGACGC TGGTTTTGGT 
GGCCACAGGG GGTGGCCCGT GCGACAGAGG CACATGCTGA GGCCTGCTGG TGGAGGAGGT 
AGTACCTTTG AAGATGCTGT TGACCACCTG GCCACAGCCA GTCAGGGGCC TGGTGGTGGG 
GGGCGTCTCC TGAGACAGGC CAGCTTGGAT GGCCTCCAGC AACTCAGGGA TAACATGGGC 
TCCAGGAGGG ACCCTCTGGT CCAGCTGCCC CACCCAGCTG CCCTTATCAG CTCCAAGTCC 
CTCTCTGAGC CTTTATTGAA CTCCCCAGAC CCACTGCTCT CCTCCTTTTC CCACCCTGAT 
TCCCCATCTT CCTCATCTCC ACCATCCACC CAGGAGGCCT CTCCCACTGG TGATATGGCT 
GTAGGATCCC CCTTGATGCA AGAGGTAGGC TCCCCGAAAG ACCCTGGAAA GTCCCTGCCA 
CCTGTACCAC CAATGGGCCT GCCCCCACCC CAGGAGTTTG GCCGGGGGAA CCCATTCATG 
CTGTTCCTCT GCCTGGCCAT CCTGCTGGAG CACCGCGACC ACATCATGCG CAATGGGCTG 
GATTATAATG AGCTGGCCAT GCACTTTGAC CGCCTTGTGC GAAAACACCA CCTGGGGCGC 
GTCCTGCGCC GGGCTAGGGC TCTCTTTGCT GATTACCTGC AGTCAGAGGT GTGGGACTCA 
GAGGAGGGGG CTGAGGCCAC AGCCGCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335194.1
CDS	437..2329
Translation	MLGTGQWATDLYSGYLAFVWKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_001348265.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348265.1

ORF Insert Sequence:

ATGCTGGGGA CTGGCCAGTG GGCCACCGAC CTCTACAGTG GCTATCTGGC CTTTGTTTGG 
AAGAAGAACT TTGGCATCAG CTACCTGGGC CGGGACCGGC TAGGACAGGA AGTTTACCTC 
TCACTTCTAT CTGACTGGGA CCTCAGCACA GCCTTTGCCA CTGCCTCCAA ACCTTACCTG 
CAATTGCGTG TAGATATTCG GCCCTCGGAG GACAGCCCAT TGCTAGAAGA CTGGGACATA 
ATCAGCCCCA AAGATGTCAT TGGCTCCGAC GTGTTGCTGG CTGAGAAACG GTCATCACTG 
ACGACTGCCG CCCTGCCCTT TACACAGTCC ATCCTCACTC AGGTGGGCCG TACCTTGTCT 
AAGGTCCAAC AAGTGCTGAG CTGGTCGTAT GGGGAAGATG TCAAGCCCTT CAAGCCACCC 
CTGAGCGATG CTGAGTTTCA CACGTACCTG AACCACGAGG GCCAGCTCTC CCGACCCGAG 
GAGTTGCGCC TGCGGATCTA TCATGGCGGT GTGGAGCCCT CGCTGCGAAA GGTGGTGTGG 
CGGTACCTGC TGAACGTGTA TCCAGATGGA CTGACAGGCC GAGAGCGGAT GGACTACATG 
AAACGCAAGA GCCGCGAGTA TGAGCAGCTC AAGAGCGAGT GGGCCCAGCG AGCGAACCCT 
GAGGACCTGG AATTCATCCG CAGCACGGTC CTCAAGGATG TACTGCGCAC TGACCGGGCC 
CACCCCTACT ATGCGGGGCC TGAGGATGGC CCACATCTAC GGGCGCTGCA CGACCTGCTC 
ACCACCTATG CCGTTACCCA CCCACAGGTG TCCTACTGCC AGGGCATGAG TGACCTTGCC 
TCACCCATCC TCGCTGTCAT GGACCATGAG GGCCATGCCT TTGTTTGCTT TTGTGGCATC 
ATGAAACGCC TGGCCGCCAA CTTCCACCCT GACGGCCGCG CCATGGCCAC CAAGTTTGCA 
CACTTGAAGC TGTTGCTGCG ACACGCTGAC CCTGACTTTT ATCAATACCT GCAAGAGGCA 
GGCGCTGATG ACCTCTTCTT CTGTTACCGC TGGCTGCTGC TGGAACTCAA GCGTGAGTTC 
GCCTTCGACG ATGCCCTCCG CATGCTTGAG GTCACTTGGA GTTCGCTGCC CCCTGATCCT 
CCTGAACATG AGGTAGAGCT GGTTGGACCC CCCAGCCAAG TGGCAGACGC TGGTTTTGGT 
GGCCACAGGG GGTGGCCCGT GCGACAGAGG CACATGCTGA GGCCTGCTGG TGGAGGAGGT 
AGTACCTTTG AAGATGCTGT TGACCACCTG GCCACAGCCA GTCAGGGGCC TGGTGGTGGG 
GGGCGTCTCC TGAGACAGGC CAGCTTGGAT GGCCTCCAGC AACTCAGGGA TAACATGGGC 
TCCAGGAGGG ACCCTCTGGT CCAGCTGCCC CACCCAGCTG CCCTTATCAG CTCCAAGTCC 
CTCTCTGAGC CTTTATTGAA CTCCCCAGAC CCACTGCTCT CCTCCTTTTC CCACCCTGAT 
TCCCCATCTT CCTCATCTCC ACCATCCACC CAGGAGGCCT CTCCCACTGG TGATATGGCT 
GTAGGATCCC CCTTGATGCA AGAGGTAGGC TCCCCGAAAG ACCCTGGAAA GTCCCTGCCA 
CCTGTACCAC CAATGGGCCT GCCCCCACCC CAGGAGTTTG GCCGGGGGAA CCCATTCATG 
CTGTTCCTCT GCCTGGCCAT CCTGCTGGAG CACCGCGACC ACATCATGCG CAATGGGCTG 
GATTATAATG AGCTGGCCAT GCACTTTGAC CGCCTTGTGC GAAAACACCA CCTGGGGCGC 
GTCCTGCGCC GGGCTAGGGC TCTCTTTGCT GATTACCTGC AGTCAGAGGT GTGGGACTCA 
GAGGAGGGGG CTGAGGCCAC AGCCGCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D25 ( NM_001348263.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335192.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu102355
Clone ID Related Accession (Same CDS sequence)	NM_001348263.1
Accession Version	NM_001348263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF196969.3 and AC115618.3. Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.223169.1, AK293875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGGTGAGGC TGGCGAAATG TGAGAGCTTC TTGCCGCCAG AGTTCCGCTC TTTTGCTGTA 
GATCCCCAGA TCACCTCGCT CGACGTGTTG CAGCACATCC TCATCCGAGC CTTTGATTTG 
AGTGGGAAGA AGAACTTTGG CATCAGCTAC CTGGGCCGGG ACCGGCTAGG ACAGGAAGTT 
TACCTCTCAC TTCTATCTGA CTGGGACCTC AGCACAGCCT TTGCCACTGC CTCCAAACCT 
TACCTGCAAT TGCGTGTAGA TATTCGGCCC TCGGAGGACA GCCCATTGCT AGAAGACTGG 
GACATAATCA GCCCCAAAGA TGTCATTGGC TCCGACGTGT TGCTGGCTGA GAAACGGTCA 
TCACTGACGA CTGCCGCCCT GCCCTTTACA CAGTCCATCC TCACTCAGGT GGGCCGTACC 
TTGTCTAAGG TCCAACAAGT GCTGAGCTGG TCGTATGGGG AAGATGTCAA GCCCTTCAAG 
CCACCCCTGA GCGATGCTGA GTTTCACACG TACCTGAACC ACGAGGGCCA GCTCTCCCGA 
CCCGAGGAGT TGCGCCTGCG GATCTATCAT GGCGGTGTGG AGCCCTCGCT GCGAAAGGTG 
GTGTGGCGGT ACCTGCTGAA CGTGTATCCA GATGGACTGA CAGGCCGAGA GCGGATGGAC 
TACATGAAAC GCAAGAGCCG CGAGTATGAG CAGCTCAAGA GCGAGTGGGC CCAGCGAGCG 
AACCCTGAGG ACCTGGAATT CATCCGCAGC ACGGTCCTCA AGGATGTACT GCGCACTGAC 
CGGGCCCACC CCTACTATGC GGGGCCTGAG GATGGCCCAC ATCTACGGGC GCTGCACGAC 
CTGCTCACCA CCTATGCCGT TACCCACCCA CAGGTGTCCT ACTGCCAGGG CATGAGTGAC 
CTTGCCTCAC CCATCCTCGC TGTCATGGAC CATGAGGGCC ATGCCTTTGT TTGCTTTTGT 
GGCATCATGA AACGCCTGGC CGCCAACTTC CACCCTGACG GCCGCGCCAT GGCCACCAAG 
TTTGCACACT TGAAGCTGTT GCTGCGACAC GCTGACCCTG ACTTTTATCA ATACCTGCAA 
GAGGCAGGCG CTGATGACCT CTTCTTCTGT TACCGCTGGC TGCTGCTGGA ACTCAAGCGT 
GAGTTCGCCT TCGACGATGC CCTCCGCATG CTTGAGGTCA CTTGGAGTTC GCTGCCCCCT 
GATCCTCCTG AACATGAGGT AGAGCTGGTT GGACCCCCCA GCCAAGTGGC AGACGCTGGT 
TTTGGTGGCC ACAGGGGGTG GCCCGTGCGA CAGAGGCACA TGCTGAGGCC TGCTGGTGGA 
GGAGGTAGTA CCTTTGAAGA TGCTGTTGAC CACCTGGCCA CAGCCAGTCA GGGGCCTGGT 
GGTGGGGGGC GTCTCCTGAG ACAGGCCAGC TTGGATGGCC TCCAGCAACT CAGGGATAAC 
ATGGGCTCCA GGAGGGACCC TCTGGTCCAG CTGCCCCACC CAGCTGCCCT TATCAGCTCC 
AAGTCCCTCT CTGAGCCTTT ATTGAACTCC CCAGACCCAC TGCTCTCCTC CTTTTCCCAC 
CCTGATTCCC CATCTTCCTC ATCTCCACCA TCCACCCAGG AGGCCTCTCC CACTGGTGAT 
ATGGCTGTAG GATCCCCCTT GATGCAAGAG GTAGGCTCCC CGAAAGACCC TGGAAAGTCC 
CTGCCACCTG TACCACCAAT GGGCCTGCCC CCACCCCAGG AGTTTGGCCG GGGGAACCCA 
TTCATGCTGT TCCTCTGCCT GGCCATCCTG CTGGAGCACC GCGACCACAT CATGCGCAAT 
GGGCTGGATT ATAATGAGCT GGCCATGCAC TTTGACCGCC TTGTGCGAAA ACACCACCTG 
GGGCGCGTCC TGCGCCGGGC TAGGGCTCTC TTTGCTGATT ACCTGCAGTC AGAGGTGTGG 
GACTCAGAGG AGGGGGCTGA GGCCACAGCC GCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335192.1
CDS	180..2258
Translation	MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKVRLAKCESFLPPEFRSFAVDPQITSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_001348263.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348263.1

ORF Insert Sequence:

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGGTGAGGC TGGCGAAATG TGAGAGCTTC TTGCCGCCAG AGTTCCGCTC TTTTGCTGTA 
GATCCCCAGA TCACCTCGCT CGACGTGTTG CAGCACATCC TCATCCGAGC CTTTGATTTG 
AGTGGGAAGA AGAACTTTGG CATCAGCTAC CTGGGCCGGG ACCGGCTAGG ACAGGAAGTT 
TACCTCTCAC TTCTATCTGA CTGGGACCTC AGCACAGCCT TTGCCACTGC CTCCAAACCT 
TACCTGCAAT TGCGTGTAGA TATTCGGCCC TCGGAGGACA GCCCATTGCT AGAAGACTGG 
GACATAATCA GCCCCAAAGA TGTCATTGGC TCCGACGTGT TGCTGGCTGA GAAACGGTCA 
TCACTGACGA CTGCCGCCCT GCCCTTTACA CAGTCCATCC TCACTCAGGT GGGCCGTACC 
TTGTCTAAGG TCCAACAAGT GCTGAGCTGG TCGTATGGGG AAGATGTCAA GCCCTTCAAG 
CCACCCCTGA GCGATGCTGA GTTTCACACG TACCTGAACC ACGAGGGCCA GCTCTCCCGA 
CCCGAGGAGT TGCGCCTGCG GATCTATCAT GGCGGTGTGG AGCCCTCGCT GCGAAAGGTG 
GTGTGGCGGT ACCTGCTGAA CGTGTATCCA GATGGACTGA CAGGCCGAGA GCGGATGGAC 
TACATGAAAC GCAAGAGCCG CGAGTATGAG CAGCTCAAGA GCGAGTGGGC CCAGCGAGCG 
AACCCTGAGG ACCTGGAATT CATCCGCAGC ACGGTCCTCA AGGATGTACT GCGCACTGAC 
CGGGCCCACC CCTACTATGC GGGGCCTGAG GATGGCCCAC ATCTACGGGC GCTGCACGAC 
CTGCTCACCA CCTATGCCGT TACCCACCCA CAGGTGTCCT ACTGCCAGGG CATGAGTGAC 
CTTGCCTCAC CCATCCTCGC TGTCATGGAC CATGAGGGCC ATGCCTTTGT TTGCTTTTGT 
GGCATCATGA AACGCCTGGC CGCCAACTTC CACCCTGACG GCCGCGCCAT GGCCACCAAG 
TTTGCACACT TGAAGCTGTT GCTGCGACAC GCTGACCCTG ACTTTTATCA ATACCTGCAA 
GAGGCAGGCG CTGATGACCT CTTCTTCTGT TACCGCTGGC TGCTGCTGGA ACTCAAGCGT 
GAGTTCGCCT TCGACGATGC CCTCCGCATG CTTGAGGTCA CTTGGAGTTC GCTGCCCCCT 
GATCCTCCTG AACATGAGGT AGAGCTGGTT GGACCCCCCA GCCAAGTGGC AGACGCTGGT 
TTTGGTGGCC ACAGGGGGTG GCCCGTGCGA CAGAGGCACA TGCTGAGGCC TGCTGGTGGA 
GGAGGTAGTA CCTTTGAAGA TGCTGTTGAC CACCTGGCCA CAGCCAGTCA GGGGCCTGGT 
GGTGGGGGGC GTCTCCTGAG ACAGGCCAGC TTGGATGGCC TCCAGCAACT CAGGGATAAC 
ATGGGCTCCA GGAGGGACCC TCTGGTCCAG CTGCCCCACC CAGCTGCCCT TATCAGCTCC 
AAGTCCCTCT CTGAGCCTTT ATTGAACTCC CCAGACCCAC TGCTCTCCTC CTTTTCCCAC 
CCTGATTCCC CATCTTCCTC ATCTCCACCA TCCACCCAGG AGGCCTCTCC CACTGGTGAT 
ATGGCTGTAG GATCCCCCTT GATGCAAGAG GTAGGCTCCC CGAAAGACCC TGGAAAGTCC 
CTGCCACCTG TACCACCAAT GGGCCTGCCC CCACCCCAGG AGTTTGGCCG GGGGAACCCA 
TTCATGCTGT TCCTCTGCCT GGCCATCCTG CTGGAGCACC GCGACCACAT CATGCGCAAT 
GGGCTGGATT ATAATGAGCT GGCCATGCAC TTTGACCGCC TTGTGCGAAA ACACCACCTG 
GGGCGCGTCC TGCGCCGGGC TAGGGCTCTC TTTGCTGATT ACCTGCAGTC AGAGGTGTGG 
GACTCAGAGG AGGGGGCTGA GGCCACAGCC GCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D25 ( NM_001348262.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335191.1 Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 1, mRNA.

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CloneID	OHu102358
Clone ID Related Accession (Same CDS sequence)	NM_001348262.1
Accession Version	NM_001348262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	TBC1 domain family member 25 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154299.1, BC101817.1, BE279778.1, AF196969.3, AC115618.3 and BU630180.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGCATGGTG ATGGGGCCCC TGGTGGCCTA CCCCATGTCT TCTCTCCCCA GAAATGTGAG 
AGCTTCTTGC CGCCAGAGTT CCGCTCTTTT GCTGTAGATC CCCAGATCAC CTCGCTCGAC 
GTGTTGCAGC ACATCCTCAT CCGAGCCTTT GATTTGAGTG GGAAGAAGAA CTTTGGCATC 
AGCTACCTGG GCCGGGACCG GCTAGGACAG GAAGTTTACC TCTCACTTCT ATCTGACTGG 
GACCTCAGCA CAGCCTTTGC CACTGCCTCC AAACCTTACC TGCAATTGCG TGTAGATATT 
CGGCCCTCGG AGGACAGCCC ATTGCTAGAA GACTGGGACA TAATCAGCCC CAAAGATGTC 
ATTGGCTCCG ACGTGTTGCT GGCTGAGAAA CGGTCATCAC TGACGACTGC CGCCCTGCCC 
TTTACACAGT CCATCCTCAC TCAGGTGGGC CGTACCTTGT CTAAGGTCCA ACAAGTGCTG 
AGCTGGTCGT ATGGGGAAGA TGTCAAGCCC TTCAAGCCAC CCCTGAGCGA TGCTGAGTTT 
CACACGTACC TGAACCACGA GGGCCAGCTC TCCCGACCCG AGGAGTTGCG CCTGCGGATC 
TATCATGGCG GTGTGGAGCC CTCGCTGCGA AAGGTGGTGT GGCGGTACCT GCTGAACGTG 
TATCCAGATG GACTGACAGG CCGAGAGCGG ATGGACTACA TGAAACGCAA GAGCCGCGAG 
TATGAGCAGC TCAAGAGCGA GTGGGCCCAG CGAGCGAACC CTGAGGACCT GGAATTCATC 
CGCAGCACGG TCCTCAAGGA TGTACTGCGC ACTGACCGGG CCCACCCCTA CTATGCGGGG 
CCTGAGGATG GCCCACATCT ACGGGCGCTG CACGACCTGC TCACCACCTA TGCCGTTACC 
CACCCACAGG TGTCCTACTG CCAGGGCATG AGTGACCTTG CCTCACCCAT CCTCGCTGTC 
ATGGACCATG AGGGCCATGC CTTTGTTTGC TTTTGTGGCA TCATGAAACG CCTGGCCGCC 
AACTTCCACC CTGACGGCCG CGCCATGGCC ACCAAGTTTG CACACTTGAA GCTGTTGCTG 
CGACACGCTG ACCCTGACTT TTATCAATAC CTGCAAGAGG CAGGCGCTGA TGACCTCTTC 
TTCTGTTACC GCTGGCTGCT GCTGGAACTC AAGCGTGAGT TCGCCTTCGA CGATGCCCTC 
CGCATGCTTG AGGTCACTTG GAGTTCGCTG CCCCCTGATC CTCCTGAACA TGAGGTAGAG 
CTGGTTGGAC CCCCCAGCCA AGTGGCAGAC GCTGGTTTTG GTGGCCACAG GGGGTGGCCC 
GTGCGACAGA GGCACATGCT GAGGCCTGCT GGTGGAGGAG GTAGTACCTT TGAAGATGCT 
GTTGACCACC TGGCCACAGC CAGTCAGGGG CCTGGTGGTG GGGGGCGTCT CCTGAGACAG 
GCCAGCTTGG ATGGCCTCCA GCAACTCAGG GATAACATGG GCTCCAGGAG GGACCCTCTG 
GTCCAGCTGC CCCACCCAGC TGCCCTTATC AGCTCCAAGT CCCTCTCTGA GCCTTTATTG 
AACTCCCCAG ACCCACTGCT CTCCTCCTTT TCCCACCCTG ATTCCCCATC TTCCTCATCT 
CCACCATCCA CCCAGGAGGC CTCTCCCACT GGTGATATGG CTGTAGGATC CCCCTTGATG 
CAAGAGGTAG GCTCCCCGAA AGACCCTGGA AAGTCCCTGC CACCTGTACC ACCAATGGGC 
CTGCCCCCAC CCCAGGAGTT TGGCCGGGGG AACCCATTCA TGCTGTTCCT CTGCCTGGCC 
ATCCTGCTGG AGCACCGCGA CCACATCATG CGCAATGGGC TGGATTATAA TGAGCTGGCC 
ATGCACTTTG ACCGCCTTGT GCGAAAACAC CACCTGGGGC GCGTCCTGCG CCGGGCTAGG 
GCTCTCTTTG CTGATTACCT GCAGTCAGAG GTGTGGGACT CAGAGGAGGG GGCTGAGGCC 
ACAGCCGCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335191.1
CDS	180..2294
Translation	MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKHGDGAPGGLPHVFSPQKCESFLPPEFRSFAVDPQITSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHPYYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMKRLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGSTFEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLSEPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPVPPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVLRRARALFADYLQSEVWDSEEGAEATAAS

Target ORF information:

RefSeq Version	NM_001348262.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 25 (TBC1D25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348262.1

ORF Insert Sequence:

ATGGCAACAG CCTCCGGGGC CTCGGACTTG TCTGGCTCCG GAGCGCCCCC GCCCGGTGTG 
GGAGCTCAGG CGGCGGCGGC CGCTGAGGAG GAGGAGCGAG AGGTGGTGCG GGTCCGAGTC 
AAGCATGGTG ATGGGGCCCC TGGTGGCCTA CCCCATGTCT TCTCTCCCCA GAAATGTGAG 
AGCTTCTTGC CGCCAGAGTT CCGCTCTTTT GCTGTAGATC CCCAGATCAC CTCGCTCGAC 
GTGTTGCAGC ACATCCTCAT CCGAGCCTTT GATTTGAGTG GGAAGAAGAA CTTTGGCATC 
AGCTACCTGG GCCGGGACCG GCTAGGACAG GAAGTTTACC TCTCACTTCT ATCTGACTGG 
GACCTCAGCA CAGCCTTTGC CACTGCCTCC AAACCTTACC TGCAATTGCG TGTAGATATT 
CGGCCCTCGG AGGACAGCCC ATTGCTAGAA GACTGGGACA TAATCAGCCC CAAAGATGTC 
ATTGGCTCCG ACGTGTTGCT GGCTGAGAAA CGGTCATCAC TGACGACTGC CGCCCTGCCC 
TTTACACAGT CCATCCTCAC TCAGGTGGGC CGTACCTTGT CTAAGGTCCA ACAAGTGCTG 
AGCTGGTCGT ATGGGGAAGA TGTCAAGCCC TTCAAGCCAC CCCTGAGCGA TGCTGAGTTT 
CACACGTACC TGAACCACGA GGGCCAGCTC TCCCGACCCG AGGAGTTGCG CCTGCGGATC 
TATCATGGCG GTGTGGAGCC CTCGCTGCGA AAGGTGGTGT GGCGGTACCT GCTGAACGTG 
TATCCAGATG GACTGACAGG CCGAGAGCGG ATGGACTACA TGAAACGCAA GAGCCGCGAG 
TATGAGCAGC TCAAGAGCGA GTGGGCCCAG CGAGCGAACC CTGAGGACCT GGAATTCATC 
CGCAGCACGG TCCTCAAGGA TGTACTGCGC ACTGACCGGG CCCACCCCTA CTATGCGGGG 
CCTGAGGATG GCCCACATCT ACGGGCGCTG CACGACCTGC TCACCACCTA TGCCGTTACC 
CACCCACAGG TGTCCTACTG CCAGGGCATG AGTGACCTTG CCTCACCCAT CCTCGCTGTC 
ATGGACCATG AGGGCCATGC CTTTGTTTGC TTTTGTGGCA TCATGAAACG CCTGGCCGCC 
AACTTCCACC CTGACGGCCG CGCCATGGCC ACCAAGTTTG CACACTTGAA GCTGTTGCTG 
CGACACGCTG ACCCTGACTT TTATCAATAC CTGCAAGAGG CAGGCGCTGA TGACCTCTTC 
TTCTGTTACC GCTGGCTGCT GCTGGAACTC AAGCGTGAGT TCGCCTTCGA CGATGCCCTC 
CGCATGCTTG AGGTCACTTG GAGTTCGCTG CCCCCTGATC CTCCTGAACA TGAGGTAGAG 
CTGGTTGGAC CCCCCAGCCA AGTGGCAGAC GCTGGTTTTG GTGGCCACAG GGGGTGGCCC 
GTGCGACAGA GGCACATGCT GAGGCCTGCT GGTGGAGGAG GTAGTACCTT TGAAGATGCT 
GTTGACCACC TGGCCACAGC CAGTCAGGGG CCTGGTGGTG GGGGGCGTCT CCTGAGACAG 
GCCAGCTTGG ATGGCCTCCA GCAACTCAGG GATAACATGG GCTCCAGGAG GGACCCTCTG 
GTCCAGCTGC CCCACCCAGC TGCCCTTATC AGCTCCAAGT CCCTCTCTGA GCCTTTATTG 
AACTCCCCAG ACCCACTGCT CTCCTCCTTT TCCCACCCTG ATTCCCCATC TTCCTCATCT 
CCACCATCCA CCCAGGAGGC CTCTCCCACT GGTGATATGG CTGTAGGATC CCCCTTGATG 
CAAGAGGTAG GCTCCCCGAA AGACCCTGGA AAGTCCCTGC CACCTGTACC ACCAATGGGC 
CTGCCCCCAC CCCAGGAGTT TGGCCGGGGG AACCCATTCA TGCTGTTCCT CTGCCTGGCC 
ATCCTGCTGG AGCACCGCGA CCACATCATG CGCAATGGGC TGGATTATAA TGAGCTGGCC 
ATGCACTTTG ACCGCCTTGT GCGAAAACAC CACCTGGGGC GCGTCCTGCG CCGGGCTAGG 
GCTCTCTTTG CTGATTACCT GCAGTCAGAG GTGTGGGACT CAGAGGAGGG GGCTGAGGCC 
ACAGCCGCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A systematic High-Content Screening microscopy approach reveals key roles for Rab33b, OATL1 and Myo6 in nanoparticle trafficking in HeLa cells.
Scientific reports628865(2016 Jul)
Panarella A,Bexiga MG,Galea G,O' Neill ED,Salvati A,Dawson KA,Simpson JC

Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1.
Autophagy12(2)312-26(2016)
Hirano S,Uemura T,Annoh H,Fujita N,Waguri S,Itoh T,Fukuda M

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.
Molecular and cellular biology32(9)1733-44(2012 May)
Popovic D,Akutsu M,Novak I,Harper JW,Behrends C,Dikic I

OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation.
The Journal of cell biology192(5)839-53(2011 Mar)
Itoh T,Kanno E,Uemura T,Waguri S,Fukuda M

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TBC1D25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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