ATP2A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP2A1
Entrez Gene ID	487
Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
Synonyms	ATP2A,SERCA1
General protein information	Preferred Names ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 Names sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 SR Ca(2+)-ATPase 1 calcium pump 1 calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013].
Disorder MIM:	108730
Disorder Html:	Brody myopathy, 601003 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173201.3	NP_775293.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform b
NM_004320.4	NP_004311.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
NM_004320.6	NP_004311.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
NM_001286075.1	NP_001273004.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform c
NM_173201.4	NP_775293.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform b
NM_004320.5	NP_004311.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP2A1	NP_775293.1
Pan troglodytes (chimpanzee)	ATP2A1	XP_001142958.3
Magnaporthe oryzae (rice blast fungus)	MGG_04550	XP_003710933.1
Arabidopsis thaliana (thale cress)	ECA3	NP_563860.1
Xenopus tropicalis (tropical clawed frog)	atp2a1	NP_001027498.1
Rattus norvegicus (Norway rat)	Atp2a1	NP_478120.1
Neurospora crassa	NCU03305	XP_964482.2
Mus musculus (house mouse)	Atp2a1	NP_031530.2
Danio rerio (zebrafish)	atp2a1	NP_001007030.1
Caenorhabditis elegans (roundworm)	sca-1	NP_499386.1
Oryza sativa (rice)	Os03g0730800	NP_001051163.2
Danio rerio (zebrafish)	atp2a1l	NP_001071001.1
Anopheles gambiae (African malaria mosquito)	ATC1_ANOGA	XP_001688815.1
Canis lupus familiaris (dog)	ATP2A1	XP_005621437.1
Bos taurus (cattle)	ATP2A1	NP_001069235.1
Drosophila melanogaster (fruit fly)	Ca-P60A	NP_726387.1

Gene Ontology
Bibliography

Related articles in PubMed

Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca
Desmond PF, Labuza A, Muriel J, Markwardt ML, Mancini AE, Rizzo MA, Bloch RJ
The Journal of biological chemistry292(26)10961-10972(2017 06)

Characterization of sarcoplasmic reticulum Ca(2+) ATPase pumps in muscle of patients with myotonic dystrophy and with hypothyroid myopathy.
Guglielmi V, Oosterhof A, Voermans NC, Cardani R, Molenaar JP, van Kuppevelt TH, Meola G, van Engelen BG, Tomelleri G, Vattemi G
Neuromuscular disorders : NMD26(6)378-85(2016 Jun)

Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
Toral-Ojeda I, Aldanondo G, Lasa-Elgarresta J, Lasa-Fern?ndez H, Fern?ndez-Torr?n R, L?pez de Munain A, Vallejo-Illarramendi A
Expert reviews in molecular medicine18e7(2016 Apr)

Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.
Benaim G, Pimentel AA, Felibertt P, Mayora A, Colman L, Sojo F, Rojas H, De Sanctis JB
Biochemical and biophysical research communications473(2)572-7(2016 Apr)

Formalin evokes calcium transients from the endoplasmatic reticulum.
Fischer MJ, Soller KJ, Sauer SK, Kalucka J, Veglia G, Reeh PW
PloS one10(4)e0123762(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that sAnk1 interacts with SLN both directly and in complex with SERCA1 and reduces SLN's inhibitory effect on SERCA1 activity.
Title: Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca

CAPN3 deficiency leads to degradation of SERCA proteins and Ca2+ dysregulation in the skeletal muscle.
Title: Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.

The sphingolipid sphingosine increases the [Ca(2+)]i by inhibiting the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), in a similar manner to thapsigargin (Tg), a specific inhibitor of this Ca(2+) pump.
Title: Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.

Thus the human SERCA1b has a different expression pattern from that of rodents and it is associated with DM2.
Title: The neonatal sarcoplasmic reticulum Ca2+-ATPase gives a clue to development and pathology in human muscles.

Formalin evokes calcium transients from the endoplasmatic reticulum via SERCA1-dependent, TRPA1-independent mechanism that may underlie formaldehyde-induced pan-neuronal excitation and subsequent inflammation.
Title: Formalin evokes calcium transients from the endoplasmatic reticulum.

The following ATP2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13839	NM_173201.4 Latest version!	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$504.50-$706.30 $1009.00
OHu14510	NM_001286075.1 Latest version!	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu18972	NM_004320.6 Latest version!	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu18972	NM_004320.4	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu13839	NM_173201.3	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$504.50-$706.30 $1009.00
OHu18972	NM_004320.5	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP2A1 ( NM_173201.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775293.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu13839
Clone ID Related Accession (Same CDS sequence)	NM_173201.3 , NM_173201.4
Accession Version	NM_173201.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647367.1, AK291314.1 and BX537784.1. On Jun 30, 2010 this sequence version replaced NM_173201.2. Transcript Variant: This variant (b) lacks an exon in the 3' coding region resulting in a frameshift and use of a downstream termination codon, compared to variant a. Isoform b has a longer and distinct C-terminus, compared to isoform a. Isoform b is also referred to as the neonatal isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG ATCCAGAAGA TGAAAGAAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775293.1
CDS	185..3190
Translation	MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK

Target ORF information:

RefSeq Version	NM_173201.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173201.3

ORF Insert Sequence:

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG ATCCAGAAGA TGAAAGAAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( NM_173201.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_775293.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu13839
Clone ID Related Accession (Same CDS sequence)	NM_173201.3 , NM_173201.4
Accession Version	NM_173201.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647367.1, AK291314.1, BX537784.1 and AC109460.3. On Jun 2, 2019 this sequence version replaced NM_173201.3. Transcript Variant: This variant (b) lacks an exon in the 3' coding region resulting in a frameshift and use of a downstream termination codon, compared to variant a. Isoform b has a longer and distinct C-terminus, compared to isoform a. Isoform b is also referred to as the neonatal isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG ATCCAGAAGA TGAAAGAAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775293.1
CDS	185..3190
Translation	MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK

Target ORF information:

RefSeq Version	NM_173201.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173201.4

ORF Insert Sequence:

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG ATCCAGAAGA TGAAAGAAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( NM_001286075.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273004.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant c, mRNA.

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CloneID	OHu14510
Clone ID Related Accession (Same CDS sequence)	NM_001286075.1
Accession Version	NM_001286075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL706356.1, AK128456.1, AC133550.2 and BX537784.1. On Oct 29, 2013 this sequence version replaced XM_005255337.1. Transcript Variant: This variant (c) differs in the 5' UTR and coding sequence compared to variant a. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG TCTACCGGGC TGACCGCAAG TCAGTGCAAA GGATCAAGGC TCGGGACATC 
GTCCCTGGGG ACATCGTGGA GGTGGCTGTG GGGGACAAAG TCCCTGCAGA CATCCGAATC 
CTCGCCATCA AATCCACCAC GCTGCGGGTT GACCAGTCCA TCCTGACAGG CGAGTCTGTA 
TCTGTCATCA AACACACGGA GCCCGTTCCT GACCCCCGAG CTGTCAACCA GGACAAGAAG 
AACATGCTTT TCTCGGGCAC CAACATTGCA GCCGGCAAGG CCTTGGGCAT CGTGGCCACC 
ACTGGTGTGG GCACCGAGAT TGGGAAGATC CGAGACCAAA TGGCTGCCAC AGAACAGGAC 
AAGACCCCCT TGCAGCAGAA GCTGGATGAG TTTGGGGAGC AGCTCTCCAA GGTCATCTCC 
CTCATCTGTG TGGCTGTCTG GCTTATCAAC ATTGGCCACT TCAACGACCC CGTCCATGGG 
GGCTCCTGGT TCCGCGGGGC CATCTACTAC TTTAAGATTG CCGTGGCCTT GGCTGTGGCT 
GCCATCCCCG AAGGTCTTCC TGCAGTCATC ACCACCTGCC TGGCCCTGGG TACCCGTCGG 
ATGGCAAAGA AGAATGCCAT TGTAAGAAGC TTGCCCTCCG TAGAGACCCT GGGCTGCACC 
TCTGTCATCT GTTCCGACAA GACAGGCACC CTCACCACCA ACCAGATGTC TGTCTGCAAG 
ATGTTTATCA TTGACAAGGT GGATGGGGAC ATCTGCCTCC TGAATGAGTT CTCCATCACC 
GGCTCCACTT ACGCTCCAGA GGGAGAGGTC TTGAAGAATG ATAAGCCAGT CCGGCCAGGG 
CAGTATGACG GGCTGGTGGA GCTGGCCACC ATCTGTGCCC TCTGCAATGA CTCCTCCTTG 
GACTTCAACG AGGCCAAAGG TGTCTATGAG AAGGTCGGCG AGGCCACCGA GACAGCACTC 
ACCACCCTGG TGGAGAAGAT GAATGTGTTC AACACGGATG TGAGAAGCCT CTCGAAGGTG 
GAGAGAGCCA ACGCCTGCAA CTCGGTGATC CGCCAGCTAA TGAAGAAGGA ATTCACCCTG 
GAGTTCTCCC GAGACAGAAA GTCCATGTCT GTCTATTGCT CCCCAGCCAA ATCTTCCCGG 
GCTGCTGTGG GCAACAAGAT GTTTGTCAAG GGTGCCCCTG AGGGCGTCAT CGACCGCTGT 
AACTATGTGC GAGTTGGCAC CACCCGGGTG CCACTGACGG GGCCGGTGAA GGAAAAGATC 
ATGGCGGTGA TCAAGGAGTG GGGCACTGGC CGGGACACCC TGCGCTGCTT GGCCCTGGCC 
ACCCGGGACA CCCCCCCGAA GCGAGAGGAA ATGGTCCTGG ATGACTCTGC CAGGTTCCTG 
GAGTATGAGA CGGACCTGAC ATTCGTGGGT GTAGTGGGCA TGCTGGACCC TCCGCGCAAG 
GAGGTCACGG GCTCCATCCA GCTGTGCCGT GACGCCGGGA TCCGGGTGAT CATGATCACT 
GGGGACAACA AGGGCACAGC CATTGCCATC TGCCGGCGAA TTGGCATCTT TGGGGAGAAC 
GAGGAGGTGG CCGATCGCGC CTACACGGGC CGAGAGTTCG ACGACCTGCC CCTGGCTGAA 
CAGCGGGAAG CCTGCCGACG TGCCTGCTGC TTCGCCCGTG TGGAGCCCTC GCACAAGTCC 
AAGATTGTGG AGTACCTGCA GTCCTACGAT GAGATCACAG CCATGACAGG TGATGGCGTC 
AATGACGCCC CTGCCCTGAA GAAGGCTGAG ATTGGCATTG CCATGGGATC TGGCACTGCC 
GTGGCCAAGA CTGCCTCTGA GATGGTGCTG GCTGACGACA ACTTCTCCAC CATCGTAGCT 
GCTGTGGAGG AGGGCCGCGC CATCTACAAC AACATGAAGC AGTTCATCCG CTACCTCATT 
TCCTCCAACG TGGGCGAGGT GGTCTGTATC TTCCTGACCG CTGCCCTGGG GCTGCCTGAG 
GCCCTGATCC CGGTGCAGCT GCTATGGGTG AACTTGGTGA CCGACGGGCT CCCAGCCACA 
GCCCTGGGCT TCAACCCACC AGACCTGGAC ATCATGGACC GCCCCCCCCG GAGCCCCAAG 
GAGCCCCTCA TCAGTGGCTG GCTCTTCTTC CGCTACATGG CAATCGGGGG CTATGTGGGT 
GCAGCCACCG TGGGAGCAGC TGCCTGGTGG TTCCTGTACG CTGAGGATGG GCCTCATGTC 
AACTACAGCC AGCTGACTCA CTTCATGCAG TGCACCGAGG ACAACACCCA CTTTGAGGGC 
ATAGACTGTG AGGTCTTCGA GGCCCCCGAG CCCATGACCA TGGCCCTGTC CGTGCTGGTG 
ACCATCGAGA TGTGCAATGC ACTGAACAGC CTGTCCGAGA ACCAGTCCCT GCTGCGGATG 
CCACCCTGGG TGAACATCTG GCTGCTGGGC TCCATCTGCC TCTCCATGTC CCTGCACTTC 
CTCATCCTCT ATGTTGACCC CCTGCCGATG ATCTTCAAGC TCCGGGCCCT GGACCTCACC 
CAGTGGCTCA TGGTCCTCAA GATCTCACTG CCAGTCATTG GGCTCGACGA AATCCTCAAG 
TTCGTTGCTC GGAACTACCT AGAGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273004.1
CDS	209..2818
Translation	MGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEG

Target ORF information:

RefSeq Version	NM_001286075.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286075.1

ORF Insert Sequence:

ATGGGGAAGG TCTACCGGGC TGACCGCAAG TCAGTGCAAA GGATCAAGGC TCGGGACATC 
GTCCCTGGGG ACATCGTGGA GGTGGCTGTG GGGGACAAAG TCCCTGCAGA CATCCGAATC 
CTCGCCATCA AATCCACCAC GCTGCGGGTT GACCAGTCCA TCCTGACAGG CGAGTCTGTA 
TCTGTCATCA AACACACGGA GCCCGTTCCT GACCCCCGAG CTGTCAACCA GGACAAGAAG 
AACATGCTTT TCTCGGGCAC CAACATTGCA GCCGGCAAGG CCTTGGGCAT CGTGGCCACC 
ACTGGTGTGG GCACCGAGAT TGGGAAGATC CGAGACCAAA TGGCTGCCAC AGAACAGGAC 
AAGACCCCCT TGCAGCAGAA GCTGGATGAG TTTGGGGAGC AGCTCTCCAA GGTCATCTCC 
CTCATCTGTG TGGCTGTCTG GCTTATCAAC ATTGGCCACT TCAACGACCC CGTCCATGGG 
GGCTCCTGGT TCCGCGGGGC CATCTACTAC TTTAAGATTG CCGTGGCCTT GGCTGTGGCT 
GCCATCCCCG AAGGTCTTCC TGCAGTCATC ACCACCTGCC TGGCCCTGGG TACCCGTCGG 
ATGGCAAAGA AGAATGCCAT TGTAAGAAGC TTGCCCTCCG TAGAGACCCT GGGCTGCACC 
TCTGTCATCT GTTCCGACAA GACAGGCACC CTCACCACCA ACCAGATGTC TGTCTGCAAG 
ATGTTTATCA TTGACAAGGT GGATGGGGAC ATCTGCCTCC TGAATGAGTT CTCCATCACC 
GGCTCCACTT ACGCTCCAGA GGGAGAGGTC TTGAAGAATG ATAAGCCAGT CCGGCCAGGG 
CAGTATGACG GGCTGGTGGA GCTGGCCACC ATCTGTGCCC TCTGCAATGA CTCCTCCTTG 
GACTTCAACG AGGCCAAAGG TGTCTATGAG AAGGTCGGCG AGGCCACCGA GACAGCACTC 
ACCACCCTGG TGGAGAAGAT GAATGTGTTC AACACGGATG TGAGAAGCCT CTCGAAGGTG 
GAGAGAGCCA ACGCCTGCAA CTCGGTGATC CGCCAGCTAA TGAAGAAGGA ATTCACCCTG 
GAGTTCTCCC GAGACAGAAA GTCCATGTCT GTCTATTGCT CCCCAGCCAA ATCTTCCCGG 
GCTGCTGTGG GCAACAAGAT GTTTGTCAAG GGTGCCCCTG AGGGCGTCAT CGACCGCTGT 
AACTATGTGC GAGTTGGCAC CACCCGGGTG CCACTGACGG GGCCGGTGAA GGAAAAGATC 
ATGGCGGTGA TCAAGGAGTG GGGCACTGGC CGGGACACCC TGCGCTGCTT GGCCCTGGCC 
ACCCGGGACA CCCCCCCGAA GCGAGAGGAA ATGGTCCTGG ATGACTCTGC CAGGTTCCTG 
GAGTATGAGA CGGACCTGAC ATTCGTGGGT GTAGTGGGCA TGCTGGACCC TCCGCGCAAG 
GAGGTCACGG GCTCCATCCA GCTGTGCCGT GACGCCGGGA TCCGGGTGAT CATGATCACT 
GGGGACAACA AGGGCACAGC CATTGCCATC TGCCGGCGAA TTGGCATCTT TGGGGAGAAC 
GAGGAGGTGG CCGATCGCGC CTACACGGGC CGAGAGTTCG ACGACCTGCC CCTGGCTGAA 
CAGCGGGAAG CCTGCCGACG TGCCTGCTGC TTCGCCCGTG TGGAGCCCTC GCACAAGTCC 
AAGATTGTGG AGTACCTGCA GTCCTACGAT GAGATCACAG CCATGACAGG TGATGGCGTC 
AATGACGCCC CTGCCCTGAA GAAGGCTGAG ATTGGCATTG CCATGGGATC TGGCACTGCC 
GTGGCCAAGA CTGCCTCTGA GATGGTGCTG GCTGACGACA ACTTCTCCAC CATCGTAGCT 
GCTGTGGAGG AGGGCCGCGC CATCTACAAC AACATGAAGC AGTTCATCCG CTACCTCATT 
TCCTCCAACG TGGGCGAGGT GGTCTGTATC TTCCTGACCG CTGCCCTGGG GCTGCCTGAG 
GCCCTGATCC CGGTGCAGCT GCTATGGGTG AACTTGGTGA CCGACGGGCT CCCAGCCACA 
GCCCTGGGCT TCAACCCACC AGACCTGGAC ATCATGGACC GCCCCCCCCG GAGCCCCAAG 
GAGCCCCTCA TCAGTGGCTG GCTCTTCTTC CGCTACATGG CAATCGGGGG CTATGTGGGT 
GCAGCCACCG TGGGAGCAGC TGCCTGGTGG TTCCTGTACG CTGAGGATGG GCCTCATGTC 
AACTACAGCC AGCTGACTCA CTTCATGCAG TGCACCGAGG ACAACACCCA CTTTGAGGGC 
ATAGACTGTG AGGTCTTCGA GGCCCCCGAG CCCATGACCA TGGCCCTGTC CGTGCTGGTG 
ACCATCGAGA TGTGCAATGC ACTGAACAGC CTGTCCGAGA ACCAGTCCCT GCTGCGGATG 
CCACCCTGGG TGAACATCTG GCTGCTGGGC TCCATCTGCC TCTCCATGTC CCTGCACTTC 
CTCATCCTCT ATGTTGACCC CCTGCCGATG ATCTTCAAGC TCCGGGCCCT GGACCTCACC 
CAGTGGCTCA TGGTCCTCAA GATCTCACTG CCAGTCATTG GGCTCGACGA AATCCTCAAG 
TTCGTTGCTC GGAACTACCT AGAGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( NM_004320.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004311.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

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CloneID	OHu18972
Clone ID Related Accession (Same CDS sequence)	NM_004320.4 , NM_004320.6 , NM_004320.5
Accession Version	NM_004320.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647367.1, AK291314.1 and BX537784.1. This sequence is a reference standard in the RefSeqGene project. On Jun 30, 2010 this sequence version replaced NM_004320.3. Transcript Variant: This variant (a) represents the longer transcript but encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537784.1, BX648544.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004311.1
CDS	185..3169
Translation	MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEG

Target ORF information:

RefSeq Version	NM_004320.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004320.4

ORF Insert Sequence:

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( NM_004320.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004311.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

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CloneID	OHu18972
Clone ID Related Accession (Same CDS sequence)	NM_004320.4 , NM_004320.6 , NM_004320.5
Accession Version	NM_004320.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647367.1, AK291314.1 and BX537784.1. On Nov 30, 2019 this sequence version replaced NM_004320.5. Transcript Variant: This variant (a) represents the longer transcript but encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647367.1, BX649072.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004311.1
CDS	185..3169
Translation	MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEG

Target ORF information:

RefSeq Version	NM_004320.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004320.6

ORF Insert Sequence:

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A1 ( NM_004320.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004311.1 Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

Price & Availability↑

CloneID	OHu18972
Clone ID Related Accession (Same CDS sequence)	NM_004320.4 , NM_004320.6 , NM_004320.5
Accession Version	NM_004320.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Homo sapiens(human)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647367.1, AK291314.1, BX537784.1 and AC109460.3. On Jun 2, 2019 this sequence version replaced NM_004320.4. Transcript Variant: This variant (a) represents the longer transcript but encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647367.1, BX537784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004311.1
CDS	185..3169
Translation	MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEG

Target ORF information:

RefSeq Version	NM_004320.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004320.5

ORF Insert Sequence:

ATGGAGGCCG CTCATGCTAA AACCACGGAG GAATGTTTGG CCTATTTTGG GGTGAGTGAG 
ACCACGGGCC TCACCCCGGA CCAAGTTAAG CGGAATCTGG AGAAATACGG CCTCAATGAG 
CTCCCTGCTG AGGAAGGGAA GACCCTGTGG GAGCTGGTGA TAGAGCAGTT TGAAGACCTC 
CTGGTGCGGA TTCTCCTCCT GGCCGCATGC ATTTCCTTCG TGCTGGCCTG GTTTGAGGAA 
GGTGAAGAGA CCATCACTGC CTTTGTTGAA CCCTTTGTCA TCCTCTTGAT CCTCATTGCC 
AATGCCATCG TGGGGGTTTG GCAGGAGCGG AACGCAGAGA ACGCCATCGA GGCCCTGAAG 
GAGTATGAGC CAGAGATGGG GAAGGTCTAC CGGGCTGACC GCAAGTCAGT GCAAAGGATC 
AAGGCTCGGG ACATCGTCCC TGGGGACATC GTGGAGGTGG CTGTGGGGGA CAAAGTCCCT 
GCAGACATCC GAATCCTCGC CATCAAATCC ACCACGCTGC GGGTTGACCA GTCCATCCTG 
ACAGGCGAGT CTGTATCTGT CATCAAACAC ACGGAGCCCG TTCCTGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTTTTCTCG GGCACCAACA TTGCAGCCGG CAAGGCCTTG 
GGCATCGTGG CCACCACTGG TGTGGGCACC GAGATTGGGA AGATCCGAGA CCAAATGGCT 
GCCACAGAAC AGGACAAGAC CCCCTTGCAG CAGAAGCTGG ATGAGTTTGG GGAGCAGCTC 
TCCAAGGTCA TCTCCCTCAT CTGTGTGGCT GTCTGGCTTA TCAACATTGG CCACTTCAAC 
GACCCCGTCC ATGGGGGCTC CTGGTTCCGC GGGGCCATCT ACTACTTTAA GATTGCCGTG 
GCCTTGGCTG TGGCTGCCAT CCCCGAAGGT CTTCCTGCAG TCATCACCAC CTGCCTGGCC 
CTGGGTACCC GTCGGATGGC AAAGAAGAAT GCCATTGTAA GAAGCTTGCC CTCCGTAGAG 
ACCCTGGGCT GCACCTCTGT CATCTGTTCC GACAAGACAG GCACCCTCAC CACCAACCAG 
ATGTCTGTCT GCAAGATGTT TATCATTGAC AAGGTGGATG GGGACATCTG CCTCCTGAAT 
GAGTTCTCCA TCACCGGCTC CACTTACGCT CCAGAGGGAG AGGTCTTGAA GAATGATAAG 
CCAGTCCGGC CAGGGCAGTA TGACGGGCTG GTGGAGCTGG CCACCATCTG TGCCCTCTGC 
AATGACTCCT CCTTGGACTT CAACGAGGCC AAAGGTGTCT ATGAGAAGGT CGGCGAGGCC 
ACCGAGACAG CACTCACCAC CCTGGTGGAG AAGATGAATG TGTTCAACAC GGATGTGAGA 
AGCCTCTCGA AGGTGGAGAG AGCCAACGCC TGCAACTCGG TGATCCGCCA GCTAATGAAG 
AAGGAATTCA CCCTGGAGTT CTCCCGAGAC AGAAAGTCCA TGTCTGTCTA TTGCTCCCCA 
GCCAAATCTT CCCGGGCTGC TGTGGGCAAC AAGATGTTTG TCAAGGGTGC CCCTGAGGGC 
GTCATCGACC GCTGTAACTA TGTGCGAGTT GGCACCACCC GGGTGCCACT GACGGGGCCG 
GTGAAGGAAA AGATCATGGC GGTGATCAAG GAGTGGGGCA CTGGCCGGGA CACCCTGCGC 
TGCTTGGCCC TGGCCACCCG GGACACCCCC CCGAAGCGAG AGGAAATGGT CCTGGATGAC 
TCTGCCAGGT TCCTGGAGTA TGAGACGGAC CTGACATTCG TGGGTGTAGT GGGCATGCTG 
GACCCTCCGC GCAAGGAGGT CACGGGCTCC ATCCAGCTGT GCCGTGACGC CGGGATCCGG 
GTGATCATGA TCACTGGGGA CAACAAGGGC ACAGCCATTG CCATCTGCCG GCGAATTGGC 
ATCTTTGGGG AGAACGAGGA GGTGGCCGAT CGCGCCTACA CGGGCCGAGA GTTCGACGAC 
CTGCCCCTGG CTGAACAGCG GGAAGCCTGC CGACGTGCCT GCTGCTTCGC CCGTGTGGAG 
CCCTCGCACA AGTCCAAGAT TGTGGAGTAC CTGCAGTCCT ACGATGAGAT CACAGCCATG 
ACAGGTGATG GCGTCAATGA CGCCCCTGCC CTGAAGAAGG CTGAGATTGG CATTGCCATG 
GGATCTGGCA CTGCCGTGGC CAAGACTGCC TCTGAGATGG TGCTGGCTGA CGACAACTTC 
TCCACCATCG TAGCTGCTGT GGAGGAGGGC CGCGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATTTCCTC CAACGTGGGC GAGGTGGTCT GTATCTTCCT GACCGCTGCC 
CTGGGGCTGC CTGAGGCCCT GATCCCGGTG CAGCTGCTAT GGGTGAACTT GGTGACCGAC 
GGGCTCCCAG CCACAGCCCT GGGCTTCAAC CCACCAGACC TGGACATCAT GGACCGCCCC 
CCCCGGAGCC CCAAGGAGCC CCTCATCAGT GGCTGGCTCT TCTTCCGCTA CATGGCAATC 
GGGGGCTATG TGGGTGCAGC CACCGTGGGA GCAGCTGCCT GGTGGTTCCT GTACGCTGAG 
GATGGGCCTC ATGTCAACTA CAGCCAGCTG ACTCACTTCA TGCAGTGCAC CGAGGACAAC 
ACCCACTTTG AGGGCATAGA CTGTGAGGTC TTCGAGGCCC CCGAGCCCAT GACCATGGCC 
CTGTCCGTGC TGGTGACCAT CGAGATGTGC AATGCACTGA ACAGCCTGTC CGAGAACCAG 
TCCCTGCTGC GGATGCCACC CTGGGTGAAC ATCTGGCTGC TGGGCTCCAT CTGCCTCTCC 
ATGTCCCTGC ACTTCCTCAT CCTCTATGTT GACCCCCTGC CGATGATCTT CAAGCTCCGG 
GCCCTGGACC TCACCCAGTG GCTCATGGTC CTCAAGATCT CACTGCCAGT CATTGGGCTC 
GACGAAATCC TCAAGTTCGT TGCTCGGAAC TACCTAGAGG GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca
The Journal of biological chemistry292(26)10961-10972(2017 06)
Desmond PF,Labuza A,Muriel J,Markwardt ML,Mancini AE,Rizzo MA,Bloch RJ

Characterization of sarcoplasmic reticulum Ca(2+) ATPase pumps in muscle of patients with myotonic dystrophy and with hypothyroid myopathy.
Neuromuscular disorders : NMD26(6)378-85(2016 Jun)
Guglielmi V,Oosterhof A,Voermans NC,Cardani R,Molenaar JP,van Kuppevelt TH,Meola G,van Engelen BG,Tomelleri G,Vattemi G

Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
Expert reviews in molecular medicine18e7(2016 Apr)
Toral-Ojeda I,Aldanondo G,Lasa-Elgarresta J,Lasa-Fern?ndez H,Fern?ndez-Torr?n R,L?pez de Munain A,Vallejo-Illarramendi A

Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.
Biochemical and biophysical research communications473(2)572-7(2016 Apr)
Benaim G,Pimentel AA,Felibertt P,Mayora A,Colman L,Sojo F,Rojas H,De Sanctis JB

Formalin evokes calcium transients from the endoplasmatic reticulum.
PloS one10(4)e0123762(2015)
Fischer MJ,Soller KJ,Sauer SK,Kalucka J,Veglia G,Reeh PW

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ATP2A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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