NOTCH2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOTCH2
Entrez Gene ID	4853
Full Name	notch 2
Synonyms	AGS2,HJCYS,hN2
General protein information	Preferred Names notch 2 Names neurogenic locus notch homolog protein 2 Notch homolog 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p12
Summary	This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011].
Disorder MIM:	600275
Disorder Html:	Alagille syndrome 2, 610205 (3); Hajdu-Cheney syndrome, 102500 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005270901.3	XP_005270958.1	neurogenic locus notch homolog protein 2 isoform X3
NM_024408.3	NP_077719.2	neurogenic locus notch homolog protein 2 isoform 1 preproprotein
XM_011541520.2	XP_011539822.1	neurogenic locus notch homolog protein 2 isoform X3
XM_017001373.1	XP_016856862.1	neurogenic locus notch homolog protein 2 isoform X3
NM_024408.4	NP_077719.2	neurogenic locus notch homolog protein 2 isoform 1 preproprotein
XM_017001372.1	XP_016856861.1	neurogenic locus notch homolog protein 2 isoform X2
NM_001200001.1	NP_001186930.1	neurogenic locus notch homolog protein 2 isoform 2 precursor
XM_011541519.2	XP_011539821.1	neurogenic locus notch homolog protein 2 isoform X1

Pathways from BioSystems

Homologies

	NOTCH2	XP_001114248.1
Rattus norvegicus (Norway rat)	Notch2	NP_077334.1
Homo sapiens (human)	NOTCH2	NP_077719.2
Canis lupus familiaris (dog)	NOTCH2	XP_005630745.1
Bos taurus (cattle)	NOTCH2	XP_002686160.2
Xenopus tropicalis (tropical clawed frog)	LOC100486344	XP_002939126.2
Pan troglodytes (chimpanzee)	NOTCH2	XP_513697.3
Mus musculus (house mouse)	Notch2	NP_035058.2
Gallus gallus (chicken)	NOTCH2	NP_001238962.1
Danio rerio (zebrafish)	notch2	NP_001108566.1

Gene Ontology

The following NOTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16899	NM_001200001.1 Latest version!	Homo sapiens notch receptor 2 (NOTCH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu19515	NM_024408.4 Latest version!	Homo sapiens notch receptor 2 (NOTCH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu33467	XM_011541520.2 Latest version!	Homo sapiens notch 2 (NOTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OHu33467	XM_005270901.3 Latest version!	Homo sapiens notch 2 (NOTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OHu33467	XM_017001373.1 Latest version!	Homo sapiens notch 2 (NOTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OHu90064	XM_017001372.1 Latest version!	Homo sapiens notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu59298	XM_011541519.2 Latest version!	Homo sapiens notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OHu19515	NM_024408.3	Homo sapiens notch 2 (NOTCH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOTCH2 ( NM_001200001.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186930.1 Homo sapiens notch receptor 2 (NOTCH2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16899
Clone ID Related Accession (Same CDS sequence)	NM_001200001.1
Accession Version	NM_001200001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1 and AF315356.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGTATG 
AAATCATCCT TATCCATTTT CCATCCAGGG CATTGTCTTA AGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186930.1
CDS	298..4005
Translation	MPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGMKSSLSIFHPGHCLKL

Target ORF information:

RefSeq Version	NM_001200001.1
Organism	Homo sapiens(human)
Definition	Homo sapiens notch receptor 2 (NOTCH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200001.1

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGTATG 
AAATCATCCT TATCCATTTT CCATCCAGGG CATTGTCTTA AGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( NM_024408.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_077719.2 Homo sapiens notch 2 (NOTCH2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19515
Clone ID Related Accession (Same CDS sequence)	NM_024408.3 , NM_024408.4
Accession Version	NM_024408.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1, AF315356.1 and AL133036.1. This sequence is a reference standard in the RefSeqGene project. On Jan 6, 2011 this sequence version replaced NM_024408.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077719.2
CDS	298..7713
Translation	MPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	NM_024408.3
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024408.3

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( NM_024408.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077719.2 Homo sapiens notch receptor 2 (NOTCH2), transcript variant 1, mRNA.

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CloneID	OHu19515
Clone ID Related Accession (Same CDS sequence)	NM_024408.3 , NM_024408.4
Accession Version	NM_024408.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1, AF315356.1 and AL133036.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024408.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000256646.7/ ENSP00000256646.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077719.2
CDS	257..7672
Translation	MPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	NM_024408.4
Organism	Homo sapiens(human)
Definition	Homo sapiens notch receptor 2 (NOTCH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024408.4

ORF Insert Sequence:

ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_005270901.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270958.1 Homo sapiens notch 2 (NOTCH2), transcript variant X3, mRNA.

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CloneID	OHu33467
Clone ID Related Accession (Same CDS sequence)	XM_005270901.3 , XM_011541520.2 , XM_017001373.1
Accession Version	XM_005270901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270958.1
CDS	2729..10027
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_005270901.3
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270901.3

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_011541520.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539822.1 Homo sapiens notch 2 (NOTCH2), transcript variant X4, mRNA.

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CloneID	OHu33467
Clone ID Related Accession (Same CDS sequence)	XM_005270901.3 , XM_011541520.2 , XM_017001373.1
Accession Version	XM_011541520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539822.1
CDS	2433..9731
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_011541520.2
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541520.2

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_017001373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856862.1 Homo sapiens notch 2 (NOTCH2), transcript variant X5, mRNA.

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CloneID	OHu33467
Clone ID Related Accession (Same CDS sequence)	XM_005270901.3 , XM_011541520.2 , XM_017001373.1
Accession Version	XM_017001373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856862.1
CDS	64..7362
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_017001373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001373.1

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_017001372.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856861.1 Homo sapiens notch 2 (NOTCH2), transcript variant X2, mRNA.

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CloneID	OHu90064
Clone ID Related Accession (Same CDS sequence)	XM_017001372.1
Accession Version	XM_017001372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCA CATTGTTGCA CAAAGCATTG CAGTGTCGAG ATGGCTATGA ACCCTGTGTA 
AATGAAGGAA TGTGTGTTAC CTACCACAAT GGCACAGGAT ACTGCAAATG TCCAGAAGGC 
TTCTTGGGGG AATATTGTCA ACATCGAGAC CCCTGTGAGA AGAACCGCTG CCAGAATGGT 
GGGACTTGTG TGGCCCAGGC CATGCTGGGG AAAGCCACGT GCCGATGTGC CTCAGGGTTT 
ACAGGAGAGG ACTGCCAGTA CTCAACATCT CATCCATGCT TTGTGTCTCG ACCCTGCCTG 
AATGGCGGCA CATGCCATAT GCTCAGCCGG GATACCTATG AGTGCACCTG TCAAGTCGGG 
TTTACAGGTA AGGAGTGCCA ATGGACGGAT GCCTGCCTGT CTCATCCCTG TGCAAATGGA 
AGTACCTGTA CCACTGTGGC CAACCAGTTC TCCTGCAAAT GCCTCACAGG CTTCACAGGG 
CAGAAATGTG AGACTGATGT CAATGAGTGT GACATTCCAG GACACTGCCA GCATGGTGGC 
ACCTGCCTCA ACCTGCCTGG TTCCTACCAG TGCCAGTGCC CTCAGGGCTT CACAGGCCAG 
TACTGTGACA GCCTGTATGT GCCCTGTGCA CCCTCACCTT GTGTCAATGG AGGCACCTGT 
CGGCAGACTG GTGACTTCAC TTTTGAGTGC AACTGCCTTC CAGGTTTTGA AGGGAGCACC 
TGTGAGAGGA ATATTGATGA CTGCCCTAAC CACAGGTGTC AGAATGGAGG GGTTTGTGTG 
GATGGGGTCA ACACTTACAA CTGCCGCTGT CCCCCACAAT GGACAGGACA GTTCTGCACA 
GAGGATGTGG ATGAATGCCT GCTGCAGCCC AATGCCTGTC AAAATGGGGG CACCTGTGCC 
AACCGCAATG GAGGCTATGG CTGTGTATGT GTCAACGGCT GGAGTGGAGA TGACTGCAGT 
GAGAACATTG ATGATTGTGC CTTCGCCTCC TGTACTCCAG GCTCCACCTG CATCGACCGT 
GTGGCCTCCT TCTCTTGCAT GTGCCCAGAG GGGAAGGCAG GTCTCCTGTG TCATCTGGAT 
GATGCATGCA TCAGCAATCC TTGCCACAAG GGGGCACTGT GTGACACCAA CCCCCTAAAT 
GGGCAATATA TTTGCACCTG CCCACAAGGC TACAAAGGGG CTGACTGCAC AGAAGATGTG 
GATGAATGTG CCATGGCCAA TAGCAATCCT TGTGAGCATG CAGGAAAATG TGTGAACACG 
GATGGCGCCT TCCACTGTGA GTGTCTGAAG GGTTATGCAG GACCTCGTTG TGAGATGGAC 
ATCAATGAGT GCCATTCAGA CCCCTGCCAG AATGATGCTA CCTGTCTGGA TAAGATTGGA 
GGCTTCACAT GTCTGTGCAT GCCAGGTTTC AAAGGTGTGC ATTGTGAATT AGAAATAAAT 
GAATGTCAGA GCAACCCTTG TGTGAACAAT GGGCAGTGTG TGGATAAAGT CAATCGTTTC 
CAGTGCCTGT GTCCTCCTGG TTTCACTGGG CCAGTTTGCC AGATTGATAT TGATGACTGT 
TCCAGTACTC CGTGTCTGAA TGGGGCAAAG TGTATCGATC ACCCGAATGG CTATGAATGC 
CAGTGTGCCA CAGGTTTCAC TGGTGTGTTG TGTGAGGAGA ACATTGACAA CTGTGACCCC 
GATCCTTGCC ACCATGGTCA GTGTCAGGAT GGTATTGATT CCTACACCTG CATCTGCAAT 
CCCGGGTACA TGGGCGCCAT CTGCAGTGAC CAGATTGATG AATGTTACAG CAGCCCTTGC 
CTGAACGATG GTCGCTGCAT TGACCTGGTC AATGGCTACC AGTGCAACTG CCAGCCAGGC 
ACGTCAGGGG TTAATTGTGA AATTAATTTT GATGACTGTG CAAGTAACCC TTGTATCCAT 
GGAATCTGTA TGGATGGCAT TAATCGCTAC AGTTGTGTCT GCTCACCAGG ATTCACAGGG 
CAGAGATGTA ACATTGACAT TGATGAGTGT GCCTCCAATC CCTGTCGCAA GGGTGCAACA 
TGTATCAACG GTGTGAATGG TTTCCGCTGT ATATGCCCCG AGGGACCCCA TCACCCCAGC 
TGCTACTCAC AGGTGAACGA ATGCCTGAGC AATCCCTGCA TCCATGGAAA CTGTACTGGA 
GGTCTCAGTG GATATAAGTG TCTCTGTGAT GCAGGCTGGG TTGGCATCAA CTGTGAAGTG 
GACAAAAATG AATGCCTTTC GAATCCATGC CAGAATGGAG GAACTTGTGA CAATCTGGTG 
AATGGATACA GGTGTACTTG CAAGAAGGGC TTTAAAGGCT ATAACTGCCA GGTGAATATT 
GATGAATGTG CCTCAAATCC ATGCCTGAAC CAAGGAACCT GCTTTGATGA CATAAGTGGC 
TACACTTGCC ACTGTGTGCT GCCATACACA GGCAAGAATT GTCAGACAGT ATTGGCTCCC 
TGTTCCCCAA ACCCTTGTGA GAATGCTGCT GTTTGCAAAG AGTCACCAAA TTTTGAGAGT 
TATACTTGCT TGTGTGCTCC TGGCTGGCAA GGTCAGCGGT GTACCATTGA CATTGACGAG 
TGTATCTCCA AGCCCTGCAT GAACCATGGT CTCTGCCATA ACACCCAGGG CAGCTACATG 
TGTGAATGTC CACCAGGCTT CAGTGGTATG GACTGTGAGG AGGACATTGA TGACTGCCTT 
GCCAATCCTT GCCAGAATGG AGGTTCCTGT ATGGATGGAG TGAATACTTT CTCCTGCCTC 
TGCCTTCCGG GTTTCACTGG GGATAAGTGC CAGACAGACA TGAATGAGTG TCTGAGTGAA 
CCCTGTAAGA ATGGAGGGAC CTGCTCTGAC TACGTCAACA GTTACACTTG CAAGTGCCAG 
GCAGGATTTG ATGGAGTCCA TTGTGAGAAC AACATCAATG AGTGCACTGA GAGCTCCTGT 
TTCAATGGTG GCACATGTGT TGATGGGATT AACTCCTTCT CTTGCTTGTG CCCTGTGGGT 
TTCACTGGAT CCTTCTGCCT CCATGAGATC AATGAATGCA GCTCTCATCC ATGCCTGAAT 
GAGGGAACGT GTGTTGATGG CCTGGGTACC TACCGCTGCA GCTGCCCCCT GGGCTACACT 
GGGAAAAACT GTCAGACCCT GGTGAATCTC TGCAGTCGGT CTCCATGTAA AAACAAAGGT 
ACTTGCGTTC AGAAAAAAGC AGAGTCCCAG TGCCTATGTC CATCTGGATG GGCTGGTGCC 
TATTGTGACG TGCCCAATGT CTCTTGTGAC ATAGCAGCCT CCAGGAGAGG TGTGCTTGTT 
GAACACTTGT GCCAGCACTC AGGTGTCTGC ATCAATGCTG GCAACACGCA TTACTGTCAG 
TGCCCCCTGG GCTATACTGG GAGCTACTGT GAGGAGCAAC TCGATGAGTG TGCGTCCAAC 
CCCTGCCAGC ACGGGGCAAC ATGCAGTGAC TTCATTGGTG GATACAGATG CGAGTGTGTC 
CCAGGCTATC AGGGTGTCAA CTGTGAGTAT GAAGTGGATG AGTGCCAGAA TCAGCCCTGC 
CAGAATGGAG GCACCTGTAT TGACCTTGTG AACCATTTCA AGTGCTCTTG CCCACCAGGC 
ACTCGGGGCC TACTCTGTGA AGAGAACATT GATGACTGTG CCCGGGGTCC CCATTGCCTT 
AATGGTGGTC AGTGCATGGA TAGGATTGGA GGCTACAGTT GTCGCTGCTT GCCTGGCTTT 
GCTGGGGAGC GTTGTGAGGG AGACATCAAC GAGTGCCTCT CCAACCCCTG CAGCTCTGAG 
GGCAGCCTGG ACTGTATACA GCTCACCAAT GACTACCTGT GTGTTTGCCG TAGTGCCTTT 
ACTGGCCGGC ACTGTGAAAC CTTCGTCGAT GTGTGTCCCC AGATGCCCTG CCTGAATGGA 
GGGACTTGTG CTGTGGCCAG TAACATGCCT GATGGTTTCA TTTGCCGTTG TCCCCCGGGA 
TTTTCCGGGG CAAGGTGCCA GAGCAGCTGT GGACAAGTGA AATGTAGGAA GGGGGAGCAG 
TGTGTGCACA CCGCCTCTGG ACCCCGCTGC TTCTGCCCCA GTCCCCGGGA CTGCGAGTCA 
GGCTGTGCCA GTAGCCCCTG CCAGCACGGG GGCAGCTGCC ACCCTCAGCG CCAGCCTCCT 
TATTACTCCT GCCAGTGTGC CCCACCATTC TCGGGTAGCC GCTGTGAACT CTACACGGCA 
CCCCCCAGCA CCCCTCCTGC CACCTGTCTG AGCCAGTATT GTGCCGACAA AGCTCGGGAT 
GGCGTCTGTG ATGAGGCCTG CAACAGCCAT GCCTGCCAGT GGGATGGGGG TGACTGTTCT 
CTCACCATGG AGAACCCCTG GGCCAACTGC TCCTCCCCAC TTCCCTGCTG GGATTATATC 
AACAACCAGT GTGATGAGCT GTGCAACACG GTCGAGTGCC TGTTTGACAA CTTTGAATGC 
CAGGGGAACA GCAAGACATG CAAGTATGAC AAATACTGTG CAGACCACTT CAAAGACAAC 
CACTGTGACC AGGGGTGCAA CAGTGAGGAG TGTGGTTGGG ATGGGCTGGA CTGTGCTGCT 
GACCAACCTG AGAACCTGGC AGAAGGTACC CTGGTTATTG TGGTATTGAT GCCACCTGAA 
CAACTGCTCC AGGATGCTCG CAGCTTCTTG CGGGCACTGG GTACCCTGCT CCACACCAAC 
CTGCGCATTA AGCGGGACTC CCAGGGGGAA CTCATGGTGT ACCCCTATTA TGGTGAGAAG 
TCAGCTGCTA TGAAGAAACA GAGGATGACA CGCAGATCCC TTCCTGGTGA ACAAGAACAG 
GAGGTGGCTG GCTCTAAAGT CTTTCTGGAA ATTGACAACC GCCAGTGTGT TCAAGACTCA 
GACCACTGCT TCAAGAACAC GGATGCAGCA GCAGCTCTCC TGGCCTCTCA CGCCATACAG 
GGGACCCTGT CATACCCTCT TGTGTCTGTC GTCAGTGAAT CCCTGACTCC AGAACGCACT 
CAGCTCCTCT ATCTCCTTGC TGTTGCTGTT GTCATCATTC TGTTTATTAT TCTGCTGGGG 
GTAATCATGG CAAAACGAAA GCGTAAGCAT GGCTCTCTCT GGCTGCCTGA AGGTTTCACT 
CTTCGCCGAG ATGCAAGCAA TCACAAGCGT CGTGAGCCAG TGGGACAGGA TGCTGTGGGG 
CTGAAAAATC TCTCAGTGCA AGTCTCAGAA GCTAACCTAA TTGGTACTGG AACAAGTGAA 
CACTGGGTCG ATGATGAAGG GCCCCAGCCA AAGAAAGTAA AGGCTGAAGA TGAGGCCTTA 
CTCTCAGAAG AAGATGACCC CATTGATCGA CGGCCATGGA CACAGCAGCA CCTTGAAGCT 
GCAGACATCC GTAGGACACC ATCGCTGGCT CTCACCCCTC CTCAGGCAGA GCAGGAGGTG 
GATGTGTTAG ATGTGAATGT CCGTGGCCCA GATGGCTGCA CCCCATTGAT GTTGGCTTCT 
CTCCGAGGAG GCAGCTCAGA TTTGAGTGAT GAAGATGAAG ATGCAGAGGA CTCTTCTGCT 
AACATCATCA CAGACTTGGT CTACCAGGGT GCCAGCCTCC AGGCCCAGAC AGACCGGACT 
GGTGAGATGG CCCTGCACCT TGCAGCCCGC TACTCACGGG CTGATGCTGC CAAGCGTCTC 
CTGGATGCAG GTGCAGATGC CAATGCCCAG GACAACATGG GCCGCTGTCC ACTCCATGCT 
GCAGTGGCAG CTGATGCCCA AGGTGTCTTC CAGATTCTGA TTCGCAACCG AGTAACTGAT 
CTAGATGCCA GGATGAATGA TGGTACTACA CCCCTGATCC TGGCTGCCCG CCTGGCTGTG 
GAGGGAATGG TGGCAGAACT GATCAACTGC CAAGCGGATG TGAATGCAGT GGATGACCAT 
GGAAAATCTG CTCTTCACTG GGCAGCTGCT GTCAATAATG TGGAGGCAAC TCTTTTGTTG 
TTGAAAAATG GGGCCAACCG AGACATGCAG GACAACAAGG AAGAGACACC TCTGTTTCTT 
GCTGCCCGGG AGGGGAGCTA TGAAGCAGCC AAGATCCTGT TAGACCATTT TGCCAATCGA 
GACATCACAG ACCATATGGA TCGTCTTCCC CGGGATGTGG CTCGGGATCG CATGCACCAT 
GACATTGTGC GCCTTCTGGA TGAATACAAT GTGACCCCAA GCCCTCCAGG CACCGTGTTG 
ACTTCTGCTC TCTCACCTGT CATCTGTGGG CCCAACAGAT CTTTCCTCAG CCTGAAGCAC 
ACCCCAATGG GCAAGAAGTC TAGACGGCCC AGTGCCAAGA GTACCATGCC TACTAGCCTC 
CCTAACCTTG CCAAGGAGGC AAAGGATGCC AAGGGTAGTA GGAGGAAGAA GTCTCTGAGT 
GAGAAGGTCC AACTGTCTGA GAGTTCAGTA ACTTTATCCC CTGTTGATTC CCTAGAATCT 
CCTCACACGT ATGTTTCCGA CACCACATCC TCTCCAATGA TTACATCCCC TGGGATCTTA 
CAGGCCTCAC CCAACCCTAT GTTGGCCACT GCCGCCCCTC CTGCCCCAGT CCATGCCCAG 
CATGCACTAT CTTTTTCTAA CCTTCATGAA ATGCAGCCTT TGGCACATGG GGCCAGCACT 
GTGCTTCCCT CAGTGAGCCA GTTGCTATCC CACCACCACA TTGTGTCTCC AGGCAGTGGC 
AGTGCTGGAA GCTTGAGTAG GCTCCATCCA GTCCCAGTCC CAGCAGATTG GATGAACCGC 
ATGGAGGTGA ATGAGACCCA GTACAATGAG ATGTTTGGTA TGGTCCTGGC TCCAGCTGAG 
GGCACCCATC CTGGCATAGC TCCCCAGAGC AGGCCACCTG AAGGGAAGCA CATAACCACC 
CCTCGGGAGC CCTTGCCCCC CATTGTGACT TTCCAGCTCA TCCCTAAAGG CAGTATTGCC 
CAACCAGCGG GGGCTCCCCA GCCTCAGTCC ACCTGCCCTC CAGCTGTTGC GGGCCCCCTG 
CCCACCATGT ACCAGATTCC AGAAATGGCC CGTTTGCCCA GTGTGGCTTT CCCCACTGCC 
ATGATGCCCC AGCAGGACGG GCAGGTAGCT CAGACCATTC TCCCAGCCTA TCATCCTTTC 
CCAGCCTCTG TGGGCAAGTA CCCCACACCC CCTTCACAGC ACAGTTATGC TTCCTCAAAT 
GCTGCTGAGC GAACACCCAG TCACAGTGGT CACCTCCAGG GTGAGCATCC CTACCTGACA 
CCATCCCCAG AGTCTCCTGA CCAGTGGTCA AGTTCATCAC CCCACTCTGC TTCTGACTGG 
TCAGATGTGA CCACCAGCCC TACCCCTGGG GGTGCTGGAG GAGGTCAGCG GGGACCTGGG 
ACACACATGT CTGAGCCACC ACACAACAAC ATGCAGGTTT ATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856861.1
CDS	16..7383
Translation	MCFTLLHKALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_017001372.1
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001372.1

ORF Insert Sequence:

ATGTGCTTCA CATTGTTGCA CAAAGCATTG CAGTGTCGAG ATGGCTATGA ACCCTGTGTA 
AATGAAGGAA TGTGTGTTAC CTACCACAAT GGCACAGGAT ACTGCAAATG TCCAGAAGGC 
TTCTTGGGGG AATATTGTCA ACATCGAGAC CCCTGTGAGA AGAACCGCTG CCAGAATGGT 
GGGACTTGTG TGGCCCAGGC CATGCTGGGG AAAGCCACGT GCCGATGTGC CTCAGGGTTT 
ACAGGAGAGG ACTGCCAGTA CTCAACATCT CATCCATGCT TTGTGTCTCG ACCCTGCCTG 
AATGGCGGCA CATGCCATAT GCTCAGCCGG GATACCTATG AGTGCACCTG TCAAGTCGGG 
TTTACAGGTA AGGAGTGCCA ATGGACGGAT GCCTGCCTGT CTCATCCCTG TGCAAATGGA 
AGTACCTGTA CCACTGTGGC CAACCAGTTC TCCTGCAAAT GCCTCACAGG CTTCACAGGG 
CAGAAATGTG AGACTGATGT CAATGAGTGT GACATTCCAG GACACTGCCA GCATGGTGGC 
ACCTGCCTCA ACCTGCCTGG TTCCTACCAG TGCCAGTGCC CTCAGGGCTT CACAGGCCAG 
TACTGTGACA GCCTGTATGT GCCCTGTGCA CCCTCACCTT GTGTCAATGG AGGCACCTGT 
CGGCAGACTG GTGACTTCAC TTTTGAGTGC AACTGCCTTC CAGGTTTTGA AGGGAGCACC 
TGTGAGAGGA ATATTGATGA CTGCCCTAAC CACAGGTGTC AGAATGGAGG GGTTTGTGTG 
GATGGGGTCA ACACTTACAA CTGCCGCTGT CCCCCACAAT GGACAGGACA GTTCTGCACA 
GAGGATGTGG ATGAATGCCT GCTGCAGCCC AATGCCTGTC AAAATGGGGG CACCTGTGCC 
AACCGCAATG GAGGCTATGG CTGTGTATGT GTCAACGGCT GGAGTGGAGA TGACTGCAGT 
GAGAACATTG ATGATTGTGC CTTCGCCTCC TGTACTCCAG GCTCCACCTG CATCGACCGT 
GTGGCCTCCT TCTCTTGCAT GTGCCCAGAG GGGAAGGCAG GTCTCCTGTG TCATCTGGAT 
GATGCATGCA TCAGCAATCC TTGCCACAAG GGGGCACTGT GTGACACCAA CCCCCTAAAT 
GGGCAATATA TTTGCACCTG CCCACAAGGC TACAAAGGGG CTGACTGCAC AGAAGATGTG 
GATGAATGTG CCATGGCCAA TAGCAATCCT TGTGAGCATG CAGGAAAATG TGTGAACACG 
GATGGCGCCT TCCACTGTGA GTGTCTGAAG GGTTATGCAG GACCTCGTTG TGAGATGGAC 
ATCAATGAGT GCCATTCAGA CCCCTGCCAG AATGATGCTA CCTGTCTGGA TAAGATTGGA 
GGCTTCACAT GTCTGTGCAT GCCAGGTTTC AAAGGTGTGC ATTGTGAATT AGAAATAAAT 
GAATGTCAGA GCAACCCTTG TGTGAACAAT GGGCAGTGTG TGGATAAAGT CAATCGTTTC 
CAGTGCCTGT GTCCTCCTGG TTTCACTGGG CCAGTTTGCC AGATTGATAT TGATGACTGT 
TCCAGTACTC CGTGTCTGAA TGGGGCAAAG TGTATCGATC ACCCGAATGG CTATGAATGC 
CAGTGTGCCA CAGGTTTCAC TGGTGTGTTG TGTGAGGAGA ACATTGACAA CTGTGACCCC 
GATCCTTGCC ACCATGGTCA GTGTCAGGAT GGTATTGATT CCTACACCTG CATCTGCAAT 
CCCGGGTACA TGGGCGCCAT CTGCAGTGAC CAGATTGATG AATGTTACAG CAGCCCTTGC 
CTGAACGATG GTCGCTGCAT TGACCTGGTC AATGGCTACC AGTGCAACTG CCAGCCAGGC 
ACGTCAGGGG TTAATTGTGA AATTAATTTT GATGACTGTG CAAGTAACCC TTGTATCCAT 
GGAATCTGTA TGGATGGCAT TAATCGCTAC AGTTGTGTCT GCTCACCAGG ATTCACAGGG 
CAGAGATGTA ACATTGACAT TGATGAGTGT GCCTCCAATC CCTGTCGCAA GGGTGCAACA 
TGTATCAACG GTGTGAATGG TTTCCGCTGT ATATGCCCCG AGGGACCCCA TCACCCCAGC 
TGCTACTCAC AGGTGAACGA ATGCCTGAGC AATCCCTGCA TCCATGGAAA CTGTACTGGA 
GGTCTCAGTG GATATAAGTG TCTCTGTGAT GCAGGCTGGG TTGGCATCAA CTGTGAAGTG 
GACAAAAATG AATGCCTTTC GAATCCATGC CAGAATGGAG GAACTTGTGA CAATCTGGTG 
AATGGATACA GGTGTACTTG CAAGAAGGGC TTTAAAGGCT ATAACTGCCA GGTGAATATT 
GATGAATGTG CCTCAAATCC ATGCCTGAAC CAAGGAACCT GCTTTGATGA CATAAGTGGC 
TACACTTGCC ACTGTGTGCT GCCATACACA GGCAAGAATT GTCAGACAGT ATTGGCTCCC 
TGTTCCCCAA ACCCTTGTGA GAATGCTGCT GTTTGCAAAG AGTCACCAAA TTTTGAGAGT 
TATACTTGCT TGTGTGCTCC TGGCTGGCAA GGTCAGCGGT GTACCATTGA CATTGACGAG 
TGTATCTCCA AGCCCTGCAT GAACCATGGT CTCTGCCATA ACACCCAGGG CAGCTACATG 
TGTGAATGTC CACCAGGCTT CAGTGGTATG GACTGTGAGG AGGACATTGA TGACTGCCTT 
GCCAATCCTT GCCAGAATGG AGGTTCCTGT ATGGATGGAG TGAATACTTT CTCCTGCCTC 
TGCCTTCCGG GTTTCACTGG GGATAAGTGC CAGACAGACA TGAATGAGTG TCTGAGTGAA 
CCCTGTAAGA ATGGAGGGAC CTGCTCTGAC TACGTCAACA GTTACACTTG CAAGTGCCAG 
GCAGGATTTG ATGGAGTCCA TTGTGAGAAC AACATCAATG AGTGCACTGA GAGCTCCTGT 
TTCAATGGTG GCACATGTGT TGATGGGATT AACTCCTTCT CTTGCTTGTG CCCTGTGGGT 
TTCACTGGAT CCTTCTGCCT CCATGAGATC AATGAATGCA GCTCTCATCC ATGCCTGAAT 
GAGGGAACGT GTGTTGATGG CCTGGGTACC TACCGCTGCA GCTGCCCCCT GGGCTACACT 
GGGAAAAACT GTCAGACCCT GGTGAATCTC TGCAGTCGGT CTCCATGTAA AAACAAAGGT 
ACTTGCGTTC AGAAAAAAGC AGAGTCCCAG TGCCTATGTC CATCTGGATG GGCTGGTGCC 
TATTGTGACG TGCCCAATGT CTCTTGTGAC ATAGCAGCCT CCAGGAGAGG TGTGCTTGTT 
GAACACTTGT GCCAGCACTC AGGTGTCTGC ATCAATGCTG GCAACACGCA TTACTGTCAG 
TGCCCCCTGG GCTATACTGG GAGCTACTGT GAGGAGCAAC TCGATGAGTG TGCGTCCAAC 
CCCTGCCAGC ACGGGGCAAC ATGCAGTGAC TTCATTGGTG GATACAGATG CGAGTGTGTC 
CCAGGCTATC AGGGTGTCAA CTGTGAGTAT GAAGTGGATG AGTGCCAGAA TCAGCCCTGC 
CAGAATGGAG GCACCTGTAT TGACCTTGTG AACCATTTCA AGTGCTCTTG CCCACCAGGC 
ACTCGGGGCC TACTCTGTGA AGAGAACATT GATGACTGTG CCCGGGGTCC CCATTGCCTT 
AATGGTGGTC AGTGCATGGA TAGGATTGGA GGCTACAGTT GTCGCTGCTT GCCTGGCTTT 
GCTGGGGAGC GTTGTGAGGG AGACATCAAC GAGTGCCTCT CCAACCCCTG CAGCTCTGAG 
GGCAGCCTGG ACTGTATACA GCTCACCAAT GACTACCTGT GTGTTTGCCG TAGTGCCTTT 
ACTGGCCGGC ACTGTGAAAC CTTCGTCGAT GTGTGTCCCC AGATGCCCTG CCTGAATGGA 
GGGACTTGTG CTGTGGCCAG TAACATGCCT GATGGTTTCA TTTGCCGTTG TCCCCCGGGA 
TTTTCCGGGG CAAGGTGCCA GAGCAGCTGT GGACAAGTGA AATGTAGGAA GGGGGAGCAG 
TGTGTGCACA CCGCCTCTGG ACCCCGCTGC TTCTGCCCCA GTCCCCGGGA CTGCGAGTCA 
GGCTGTGCCA GTAGCCCCTG CCAGCACGGG GGCAGCTGCC ACCCTCAGCG CCAGCCTCCT 
TATTACTCCT GCCAGTGTGC CCCACCATTC TCGGGTAGCC GCTGTGAACT CTACACGGCA 
CCCCCCAGCA CCCCTCCTGC CACCTGTCTG AGCCAGTATT GTGCCGACAA AGCTCGGGAT 
GGCGTCTGTG ATGAGGCCTG CAACAGCCAT GCCTGCCAGT GGGATGGGGG TGACTGTTCT 
CTCACCATGG AGAACCCCTG GGCCAACTGC TCCTCCCCAC TTCCCTGCTG GGATTATATC 
AACAACCAGT GTGATGAGCT GTGCAACACG GTCGAGTGCC TGTTTGACAA CTTTGAATGC 
CAGGGGAACA GCAAGACATG CAAGTATGAC AAATACTGTG CAGACCACTT CAAAGACAAC 
CACTGTGACC AGGGGTGCAA CAGTGAGGAG TGTGGTTGGG ATGGGCTGGA CTGTGCTGCT 
GACCAACCTG AGAACCTGGC AGAAGGTACC CTGGTTATTG TGGTATTGAT GCCACCTGAA 
CAACTGCTCC AGGATGCTCG CAGCTTCTTG CGGGCACTGG GTACCCTGCT CCACACCAAC 
CTGCGCATTA AGCGGGACTC CCAGGGGGAA CTCATGGTGT ACCCCTATTA TGGTGAGAAG 
TCAGCTGCTA TGAAGAAACA GAGGATGACA CGCAGATCCC TTCCTGGTGA ACAAGAACAG 
GAGGTGGCTG GCTCTAAAGT CTTTCTGGAA ATTGACAACC GCCAGTGTGT TCAAGACTCA 
GACCACTGCT TCAAGAACAC GGATGCAGCA GCAGCTCTCC TGGCCTCTCA CGCCATACAG 
GGGACCCTGT CATACCCTCT TGTGTCTGTC GTCAGTGAAT CCCTGACTCC AGAACGCACT 
CAGCTCCTCT ATCTCCTTGC TGTTGCTGTT GTCATCATTC TGTTTATTAT TCTGCTGGGG 
GTAATCATGG CAAAACGAAA GCGTAAGCAT GGCTCTCTCT GGCTGCCTGA AGGTTTCACT 
CTTCGCCGAG ATGCAAGCAA TCACAAGCGT CGTGAGCCAG TGGGACAGGA TGCTGTGGGG 
CTGAAAAATC TCTCAGTGCA AGTCTCAGAA GCTAACCTAA TTGGTACTGG AACAAGTGAA 
CACTGGGTCG ATGATGAAGG GCCCCAGCCA AAGAAAGTAA AGGCTGAAGA TGAGGCCTTA 
CTCTCAGAAG AAGATGACCC CATTGATCGA CGGCCATGGA CACAGCAGCA CCTTGAAGCT 
GCAGACATCC GTAGGACACC ATCGCTGGCT CTCACCCCTC CTCAGGCAGA GCAGGAGGTG 
GATGTGTTAG ATGTGAATGT CCGTGGCCCA GATGGCTGCA CCCCATTGAT GTTGGCTTCT 
CTCCGAGGAG GCAGCTCAGA TTTGAGTGAT GAAGATGAAG ATGCAGAGGA CTCTTCTGCT 
AACATCATCA CAGACTTGGT CTACCAGGGT GCCAGCCTCC AGGCCCAGAC AGACCGGACT 
GGTGAGATGG CCCTGCACCT TGCAGCCCGC TACTCACGGG CTGATGCTGC CAAGCGTCTC 
CTGGATGCAG GTGCAGATGC CAATGCCCAG GACAACATGG GCCGCTGTCC ACTCCATGCT 
GCAGTGGCAG CTGATGCCCA AGGTGTCTTC CAGATTCTGA TTCGCAACCG AGTAACTGAT 
CTAGATGCCA GGATGAATGA TGGTACTACA CCCCTGATCC TGGCTGCCCG CCTGGCTGTG 
GAGGGAATGG TGGCAGAACT GATCAACTGC CAAGCGGATG TGAATGCAGT GGATGACCAT 
GGAAAATCTG CTCTTCACTG GGCAGCTGCT GTCAATAATG TGGAGGCAAC TCTTTTGTTG 
TTGAAAAATG GGGCCAACCG AGACATGCAG GACAACAAGG AAGAGACACC TCTGTTTCTT 
GCTGCCCGGG AGGGGAGCTA TGAAGCAGCC AAGATCCTGT TAGACCATTT TGCCAATCGA 
GACATCACAG ACCATATGGA TCGTCTTCCC CGGGATGTGG CTCGGGATCG CATGCACCAT 
GACATTGTGC GCCTTCTGGA TGAATACAAT GTGACCCCAA GCCCTCCAGG CACCGTGTTG 
ACTTCTGCTC TCTCACCTGT CATCTGTGGG CCCAACAGAT CTTTCCTCAG CCTGAAGCAC 
ACCCCAATGG GCAAGAAGTC TAGACGGCCC AGTGCCAAGA GTACCATGCC TACTAGCCTC 
CCTAACCTTG CCAAGGAGGC AAAGGATGCC AAGGGTAGTA GGAGGAAGAA GTCTCTGAGT 
GAGAAGGTCC AACTGTCTGA GAGTTCAGTA ACTTTATCCC CTGTTGATTC CCTAGAATCT 
CCTCACACGT ATGTTTCCGA CACCACATCC TCTCCAATGA TTACATCCCC TGGGATCTTA 
CAGGCCTCAC CCAACCCTAT GTTGGCCACT GCCGCCCCTC CTGCCCCAGT CCATGCCCAG 
CATGCACTAT CTTTTTCTAA CCTTCATGAA ATGCAGCCTT TGGCACATGG GGCCAGCACT 
GTGCTTCCCT CAGTGAGCCA GTTGCTATCC CACCACCACA TTGTGTCTCC AGGCAGTGGC 
AGTGCTGGAA GCTTGAGTAG GCTCCATCCA GTCCCAGTCC CAGCAGATTG GATGAACCGC 
ATGGAGGTGA ATGAGACCCA GTACAATGAG ATGTTTGGTA TGGTCCTGGC TCCAGCTGAG 
GGCACCCATC CTGGCATAGC TCCCCAGAGC AGGCCACCTG AAGGGAAGCA CATAACCACC 
CCTCGGGAGC CCTTGCCCCC CATTGTGACT TTCCAGCTCA TCCCTAAAGG CAGTATTGCC 
CAACCAGCGG GGGCTCCCCA GCCTCAGTCC ACCTGCCCTC CAGCTGTTGC GGGCCCCCTG 
CCCACCATGT ACCAGATTCC AGAAATGGCC CGTTTGCCCA GTGTGGCTTT CCCCACTGCC 
ATGATGCCCC AGCAGGACGG GCAGGTAGCT CAGACCATTC TCCCAGCCTA TCATCCTTTC 
CCAGCCTCTG TGGGCAAGTA CCCCACACCC CCTTCACAGC ACAGTTATGC TTCCTCAAAT 
GCTGCTGAGC GAACACCCAG TCACAGTGGT CACCTCCAGG GTGAGCATCC CTACCTGACA 
CCATCCCCAG AGTCTCCTGA CCAGTGGTCA AGTTCATCAC CCCACTCTGC TTCTGACTGG 
TCAGATGTGA CCACCAGCCC TACCCCTGGG GGTGCTGGAG GAGGTCAGCG GGGACCTGGG 
ACACACATGT CTGAGCCACC ACACAACAAC ATGCAGGTTT ATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_011541519.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539821.1 Homo sapiens notch 2 (NOTCH2), transcript variant X1, mRNA.

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CloneID	OHu59298
Clone ID Related Accession (Same CDS sequence)	XM_011541519.2
Accession Version	XM_011541519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT ATTTGGTGTC GGCAGTTTCC CTCAGATGTG TTAGTGTGGA GAGATATAAA 
GCATTGCAGT GTCGAGATGG CTATGAACCC TGTGTAAATG AAGGAATGTG TGTTACCTAC 
CACAATGGCA CAGGATACTG CAAATGTCCA GAAGGCTTCT TGGGGGAATA TTGTCAACAT 
CGAGACCCCT GTGAGAAGAA CCGCTGCCAG AATGGTGGGA CTTGTGTGGC CCAGGCCATG 
CTGGGGAAAG CCACGTGCCG ATGTGCCTCA GGGTTTACAG GAGAGGACTG CCAGTACTCA 
ACATCTCATC CATGCTTTGT GTCTCGACCC TGCCTGAATG GCGGCACATG CCATATGCTC 
AGCCGGGATA CCTATGAGTG CACCTGTCAA GTCGGGTTTA CAGGTAAGGA GTGCCAATGG 
ACGGATGCCT GCCTGTCTCA TCCCTGTGCA AATGGAAGTA CCTGTACCAC TGTGGCCAAC 
CAGTTCTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA AATGTGAGAC TGATGTCAAT 
GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCACCT GCCTCAACCT GCCTGGTTCC 
TACCAGTGCC AGTGCCCTCA GGGCTTCACA GGCCAGTACT GTGACAGCCT GTATGTGCCC 
TGTGCACCCT CACCTTGTGT CAATGGAGGC ACCTGTCGGC AGACTGGTGA CTTCACTTTT 
GAGTGCAACT GCCTTCCAGG TTTTGAAGGG AGCACCTGTG AGAGGAATAT TGATGACTGC 
CCTAACCACA GGTGTCAGAA TGGAGGGGTT TGTGTGGATG GGGTCAACAC TTACAACTGC 
CGCTGTCCCC CACAATGGAC AGGACAGTTC TGCACAGAGG ATGTGGATGA ATGCCTGCTG 
CAGCCCAATG CCTGTCAAAA TGGGGGCACC TGTGCCAACC GCAATGGAGG CTATGGCTGT 
GTATGTGTCA ACGGCTGGAG TGGAGATGAC TGCAGTGAGA ACATTGATGA TTGTGCCTTC 
GCCTCCTGTA CTCCAGGCTC CACCTGCATC GACCGTGTGG CCTCCTTCTC TTGCATGTGC 
CCAGAGGGGA AGGCAGGTCT CCTGTGTCAT CTGGATGATG CATGCATCAG CAATCCTTGC 
CACAAGGGGG CACTGTGTGA CACCAACCCC CTAAATGGGC AATATATTTG CACCTGCCCA 
CAAGGCTACA AAGGGGCTGA CTGCACAGAA GATGTGGATG AATGTGCCAT GGCCAATAGC 
AATCCTTGTG AGCATGCAGG AAAATGTGTG AACACGGATG GCGCCTTCCA CTGTGAGTGT 
CTGAAGGGTT ATGCAGGACC TCGTTGTGAG ATGGACATCA ATGAGTGCCA TTCAGACCCC 
TGCCAGAATG ATGCTACCTG TCTGGATAAG ATTGGAGGCT TCACATGTCT GTGCATGCCA 
GGTTTCAAAG GTGTGCATTG TGAATTAGAA ATAAATGAAT GTCAGAGCAA CCCTTGTGTG 
AACAATGGGC AGTGTGTGGA TAAAGTCAAT CGTTTCCAGT GCCTGTGTCC TCCTGGTTTC 
ACTGGGCCAG TTTGCCAGAT TGATATTGAT GACTGTTCCA GTACTCCGTG TCTGAATGGG 
GCAAAGTGTA TCGATCACCC GAATGGCTAT GAATGCCAGT GTGCCACAGG TTTCACTGGT 
GTGTTGTGTG AGGAGAACAT TGACAACTGT GACCCCGATC CTTGCCACCA TGGTCAGTGT 
CAGGATGGTA TTGATTCCTA CACCTGCATC TGCAATCCCG GGTACATGGG CGCCATCTGC 
AGTGACCAGA TTGATGAATG TTACAGCAGC CCTTGCCTGA ACGATGGTCG CTGCATTGAC 
CTGGTCAATG GCTACCAGTG CAACTGCCAG CCAGGCACGT CAGGGGTTAA TTGTGAAATT 
AATTTTGATG ACTGTGCAAG TAACCCTTGT ATCCATGGAA TCTGTATGGA TGGCATTAAT 
CGCTACAGTT GTGTCTGCTC ACCAGGATTC ACAGGGCAGA GATGTAACAT TGACATTGAT 
GAGTGTGCCT CCAATCCCTG TCGCAAGGGT GCAACATGTA TCAACGGTGT GAATGGTTTC 
CGCTGTATAT GCCCCGAGGG ACCCCATCAC CCCAGCTGCT ACTCACAGGT GAACGAATGC 
CTGAGCAATC CCTGCATCCA TGGAAACTGT ACTGGAGGTC TCAGTGGATA TAAGTGTCTC 
TGTGATGCAG GCTGGGTTGG CATCAACTGT GAAGTGGACA AAAATGAATG CCTTTCGAAT 
CCATGCCAGA ATGGAGGAAC TTGTGACAAT CTGGTGAATG GATACAGGTG TACTTGCAAG 
AAGGGCTTTA AAGGCTATAA CTGCCAGGTG AATATTGATG AATGTGCCTC AAATCCATGC 
CTGAACCAAG GAACCTGCTT TGATGACATA AGTGGCTACA CTTGCCACTG TGTGCTGCCA 
TACACAGGCA AGAATTGTCA GACAGTATTG GCTCCCTGTT CCCCAAACCC TTGTGAGAAT 
GCTGCTGTTT GCAAAGAGTC ACCAAATTTT GAGAGTTATA CTTGCTTGTG TGCTCCTGGC 
TGGCAAGGTC AGCGGTGTAC CATTGACATT GACGAGTGTA TCTCCAAGCC CTGCATGAAC 
CATGGTCTCT GCCATAACAC CCAGGGCAGC TACATGTGTG AATGTCCACC AGGCTTCAGT 
GGTATGGACT GTGAGGAGGA CATTGATGAC TGCCTTGCCA ATCCTTGCCA GAATGGAGGT 
TCCTGTATGG ATGGAGTGAA TACTTTCTCC TGCCTCTGCC TTCCGGGTTT CACTGGGGAT 
AAGTGCCAGA CAGACATGAA TGAGTGTCTG AGTGAACCCT GTAAGAATGG AGGGACCTGC 
TCTGACTACG TCAACAGTTA CACTTGCAAG TGCCAGGCAG GATTTGATGG AGTCCATTGT 
GAGAACAACA TCAATGAGTG CACTGAGAGC TCCTGTTTCA ATGGTGGCAC ATGTGTTGAT 
GGGATTAACT CCTTCTCTTG CTTGTGCCCT GTGGGTTTCA CTGGATCCTT CTGCCTCCAT 
GAGATCAATG AATGCAGCTC TCATCCATGC CTGAATGAGG GAACGTGTGT TGATGGCCTG 
GGTACCTACC GCTGCAGCTG CCCCCTGGGC TACACTGGGA AAAACTGTCA GACCCTGGTG 
AATCTCTGCA GTCGGTCTCC ATGTAAAAAC AAAGGTACTT GCGTTCAGAA AAAAGCAGAG 
TCCCAGTGCC TATGTCCATC TGGATGGGCT GGTGCCTATT GTGACGTGCC CAATGTCTCT 
TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC ACTTGTGCCA GCACTCAGGT 
GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC CCCTGGGCTA TACTGGGAGC 
TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT GCCAGCACGG GGCAACATGC 
AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG GCTATCAGGG TGTCAACTGT 
GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA ATGGAGGCAC CTGTATTGAC 
CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC GGGGCCTACT CTGTGAAGAG 
AACATTGATG ACTGTGCCCG GGGTCCCCAT TGCCTTAATG GTGGTCAGTG CATGGATAGG 
ATTGGAGGCT ACAGTTGTCG CTGCTTGCCT GGCTTTGCTG GGGAGCGTTG TGAGGGAGAC 
ATCAACGAGT GCCTCTCCAA CCCCTGCAGC TCTGAGGGCA GCCTGGACTG TATACAGCTC 
ACCAATGACT ACCTGTGTGT TTGCCGTAGT GCCTTTACTG GCCGGCACTG TGAAACCTTC 
GTCGATGTGT GTCCCCAGAT GCCCTGCCTG AATGGAGGGA CTTGTGCTGT GGCCAGTAAC 
ATGCCTGATG GTTTCATTTG CCGTTGTCCC CCGGGATTTT CCGGGGCAAG GTGCCAGAGC 
AGCTGTGGAC AAGTGAAATG TAGGAAGGGG GAGCAGTGTG TGCACACCGC CTCTGGACCC 
CGCTGCTTCT GCCCCAGTCC CCGGGACTGC GAGTCAGGCT GTGCCAGTAG CCCCTGCCAG 
CACGGGGGCA GCTGCCACCC TCAGCGCCAG CCTCCTTATT ACTCCTGCCA GTGTGCCCCA 
CCATTCTCGG GTAGCCGCTG TGAACTCTAC ACGGCACCCC CCAGCACCCC TCCTGCCACC 
TGTCTGAGCC AGTATTGTGC CGACAAAGCT CGGGATGGCG TCTGTGATGA GGCCTGCAAC 
AGCCATGCCT GCCAGTGGGA TGGGGGTGAC TGTTCTCTCA CCATGGAGAA CCCCTGGGCC 
AACTGCTCCT CCCCACTTCC CTGCTGGGAT TATATCAACA ACCAGTGTGA TGAGCTGTGC 
AACACGGTCG AGTGCCTGTT TGACAACTTT GAATGCCAGG GGAACAGCAA GACATGCAAG 
TATGACAAAT ACTGTGCAGA CCACTTCAAA GACAACCACT GTGACCAGGG GTGCAACAGT 
GAGGAGTGTG GTTGGGATGG GCTGGACTGT GCTGCTGACC AACCTGAGAA CCTGGCAGAA 
GGTACCCTGG TTATTGTGGT ATTGATGCCA CCTGAACAAC TGCTCCAGGA TGCTCGCAGC 
TTCTTGCGGG CACTGGGTAC CCTGCTCCAC ACCAACCTGC GCATTAAGCG GGACTCCCAG 
GGGGAACTCA TGGTGTACCC CTATTATGGT GAGAAGTCAG CTGCTATGAA GAAACAGAGG 
ATGACACGCA GATCCCTTCC TGGTGAACAA GAACAGGAGG TGGCTGGCTC TAAAGTCTTT 
CTGGAAATTG ACAACCGCCA GTGTGTTCAA GACTCAGACC ACTGCTTCAA GAACACGGAT 
GCAGCAGCAG CTCTCCTGGC CTCTCACGCC ATACAGGGGA CCCTGTCATA CCCTCTTGTG 
TCTGTCGTCA GTGAATCCCT GACTCCAGAA CGCACTCAGC TCCTCTATCT CCTTGCTGTT 
GCTGTTGTCA TCATTCTGTT TATTATTCTG CTGGGGGTAA TCATGGCAAA ACGAAAGCGT 
AAGCATGGCT CTCTCTGGCT GCCTGAAGGT TTCACTCTTC GCCGAGATGC AAGCAATCAC 
AAGCGTCGTG AGCCAGTGGG ACAGGATGCT GTGGGGCTGA AAAATCTCTC AGTGCAAGTC 
TCAGAAGCTA ACCTAATTGG TACTGGAACA AGTGAACACT GGGTCGATGA TGAAGGGCCC 
CAGCCAAAGA AAGTAAAGGC TGAAGATGAG GCCTTACTCT CAGAAGAAGA TGACCCCATT 
GATCGACGGC CATGGACACA GCAGCACCTT GAAGCTGCAG ACATCCGTAG GACACCATCG 
CTGGCTCTCA CCCCTCCTCA GGCAGAGCAG GAGGTGGATG TGTTAGATGT GAATGTCCGT 
GGCCCAGATG GCTGCACCCC ATTGATGTTG GCTTCTCTCC GAGGAGGCAG CTCAGATTTG 
AGTGATGAAG ATGAAGATGC AGAGGACTCT TCTGCTAACA TCATCACAGA CTTGGTCTAC 
CAGGGTGCCA GCCTCCAGGC CCAGACAGAC CGGACTGGTG AGATGGCCCT GCACCTTGCA 
GCCCGCTACT CACGGGCTGA TGCTGCCAAG CGTCTCCTGG ATGCAGGTGC AGATGCCAAT 
GCCCAGGACA ACATGGGCCG CTGTCCACTC CATGCTGCAG TGGCAGCTGA TGCCCAAGGT 
GTCTTCCAGA TTCTGATTCG CAACCGAGTA ACTGATCTAG ATGCCAGGAT GAATGATGGT 
ACTACACCCC TGATCCTGGC TGCCCGCCTG GCTGTGGAGG GAATGGTGGC AGAACTGATC 
AACTGCCAAG CGGATGTGAA TGCAGTGGAT GACCATGGAA AATCTGCTCT TCACTGGGCA 
GCTGCTGTCA ATAATGTGGA GGCAACTCTT TTGTTGTTGA AAAATGGGGC CAACCGAGAC 
ATGCAGGACA ACAAGGAAGA GACACCTCTG TTTCTTGCTG CCCGGGAGGG GAGCTATGAA 
GCAGCCAAGA TCCTGTTAGA CCATTTTGCC AATCGAGACA TCACAGACCA TATGGATCGT 
CTTCCCCGGG ATGTGGCTCG GGATCGCATG CACCATGACA TTGTGCGCCT TCTGGATGAA 
TACAATGTGA CCCCAAGCCC TCCAGGCACC GTGTTGACTT CTGCTCTCTC ACCTGTCATC 
TGTGGGCCCA ACAGATCTTT CCTCAGCCTG AAGCACACCC CAATGGGCAA GAAGTCTAGA 
CGGCCCAGTG CCAAGAGTAC CATGCCTACT AGCCTCCCTA ACCTTGCCAA GGAGGCAAAG 
GATGCCAAGG GTAGTAGGAG GAAGAAGTCT CTGAGTGAGA AGGTCCAACT GTCTGAGAGT 
TCAGTAACTT TATCCCCTGT TGATTCCCTA GAATCTCCTC ACACGTATGT TTCCGACACC 
ACATCCTCTC CAATGATTAC ATCCCCTGGG ATCTTACAGG CCTCACCCAA CCCTATGTTG 
GCCACTGCCG CCCCTCCTGC CCCAGTCCAT GCCCAGCATG CACTATCTTT TTCTAACCTT 
CATGAAATGC AGCCTTTGGC ACATGGGGCC AGCACTGTGC TTCCCTCAGT GAGCCAGTTG 
CTATCCCACC ACCACATTGT GTCTCCAGGC AGTGGCAGTG CTGGAAGCTT GAGTAGGCTC 
CATCCAGTCC CAGTCCCAGC AGATTGGATG AACCGCATGG AGGTGAATGA GACCCAGTAC 
AATGAGATGT TTGGTATGGT CCTGGCTCCA GCTGAGGGCA CCCATCCTGG CATAGCTCCC 
CAGAGCAGGC CACCTGAAGG GAAGCACATA ACCACCCCTC GGGAGCCCTT GCCCCCCATT 
GTGACTTTCC AGCTCATCCC TAAAGGCAGT ATTGCCCAAC CAGCGGGGGC TCCCCAGCCT 
CAGTCCACCT GCCCTCCAGC TGTTGCGGGC CCCCTGCCCA CCATGTACCA GATTCCAGAA 
ATGGCCCGTT TGCCCAGTGT GGCTTTCCCC ACTGCCATGA TGCCCCAGCA GGACGGGCAG 
GTAGCTCAGA CCATTCTCCC AGCCTATCAT CCTTTCCCAG CCTCTGTGGG CAAGTACCCC 
ACACCCCCTT CACAGCACAG TTATGCTTCC TCAAATGCTG CTGAGCGAAC ACCCAGTCAC 
AGTGGTCACC TCCAGGGTGA GCATCCCTAC CTGACACCAT CCCCAGAGTC TCCTGACCAG 
TGGTCAAGTT CATCACCCCA CTCTGCTTCT GACTGGTCAG ATGTGACCAC CAGCCCTACC 
CCTGGGGGTG CTGGAGGAGG TCAGCGGGGA CCTGGGACAC ACATGTCTGA GCCACCACAC 
AACAACATGC AGGTTTATGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539821.1
CDS	5418..12821
Translation	MNTYLVSAVSLRCVSVERYKALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_011541519.2
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541519.2

ORF Insert Sequence:

ATGAACACAT ATTTGGTGTC GGCAGTTTCC CTCAGATGTG TTAGTGTGGA GAGATATAAA 
GCATTGCAGT GTCGAGATGG CTATGAACCC TGTGTAAATG AAGGAATGTG TGTTACCTAC 
CACAATGGCA CAGGATACTG CAAATGTCCA GAAGGCTTCT TGGGGGAATA TTGTCAACAT 
CGAGACCCCT GTGAGAAGAA CCGCTGCCAG AATGGTGGGA CTTGTGTGGC CCAGGCCATG 
CTGGGGAAAG CCACGTGCCG ATGTGCCTCA GGGTTTACAG GAGAGGACTG CCAGTACTCA 
ACATCTCATC CATGCTTTGT GTCTCGACCC TGCCTGAATG GCGGCACATG CCATATGCTC 
AGCCGGGATA CCTATGAGTG CACCTGTCAA GTCGGGTTTA CAGGTAAGGA GTGCCAATGG 
ACGGATGCCT GCCTGTCTCA TCCCTGTGCA AATGGAAGTA CCTGTACCAC TGTGGCCAAC 
CAGTTCTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA AATGTGAGAC TGATGTCAAT 
GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCACCT GCCTCAACCT GCCTGGTTCC 
TACCAGTGCC AGTGCCCTCA GGGCTTCACA GGCCAGTACT GTGACAGCCT GTATGTGCCC 
TGTGCACCCT CACCTTGTGT CAATGGAGGC ACCTGTCGGC AGACTGGTGA CTTCACTTTT 
GAGTGCAACT GCCTTCCAGG TTTTGAAGGG AGCACCTGTG AGAGGAATAT TGATGACTGC 
CCTAACCACA GGTGTCAGAA TGGAGGGGTT TGTGTGGATG GGGTCAACAC TTACAACTGC 
CGCTGTCCCC CACAATGGAC AGGACAGTTC TGCACAGAGG ATGTGGATGA ATGCCTGCTG 
CAGCCCAATG CCTGTCAAAA TGGGGGCACC TGTGCCAACC GCAATGGAGG CTATGGCTGT 
GTATGTGTCA ACGGCTGGAG TGGAGATGAC TGCAGTGAGA ACATTGATGA TTGTGCCTTC 
GCCTCCTGTA CTCCAGGCTC CACCTGCATC GACCGTGTGG CCTCCTTCTC TTGCATGTGC 
CCAGAGGGGA AGGCAGGTCT CCTGTGTCAT CTGGATGATG CATGCATCAG CAATCCTTGC 
CACAAGGGGG CACTGTGTGA CACCAACCCC CTAAATGGGC AATATATTTG CACCTGCCCA 
CAAGGCTACA AAGGGGCTGA CTGCACAGAA GATGTGGATG AATGTGCCAT GGCCAATAGC 
AATCCTTGTG AGCATGCAGG AAAATGTGTG AACACGGATG GCGCCTTCCA CTGTGAGTGT 
CTGAAGGGTT ATGCAGGACC TCGTTGTGAG ATGGACATCA ATGAGTGCCA TTCAGACCCC 
TGCCAGAATG ATGCTACCTG TCTGGATAAG ATTGGAGGCT TCACATGTCT GTGCATGCCA 
GGTTTCAAAG GTGTGCATTG TGAATTAGAA ATAAATGAAT GTCAGAGCAA CCCTTGTGTG 
AACAATGGGC AGTGTGTGGA TAAAGTCAAT CGTTTCCAGT GCCTGTGTCC TCCTGGTTTC 
ACTGGGCCAG TTTGCCAGAT TGATATTGAT GACTGTTCCA GTACTCCGTG TCTGAATGGG 
GCAAAGTGTA TCGATCACCC GAATGGCTAT GAATGCCAGT GTGCCACAGG TTTCACTGGT 
GTGTTGTGTG AGGAGAACAT TGACAACTGT GACCCCGATC CTTGCCACCA TGGTCAGTGT 
CAGGATGGTA TTGATTCCTA CACCTGCATC TGCAATCCCG GGTACATGGG CGCCATCTGC 
AGTGACCAGA TTGATGAATG TTACAGCAGC CCTTGCCTGA ACGATGGTCG CTGCATTGAC 
CTGGTCAATG GCTACCAGTG CAACTGCCAG CCAGGCACGT CAGGGGTTAA TTGTGAAATT 
AATTTTGATG ACTGTGCAAG TAACCCTTGT ATCCATGGAA TCTGTATGGA TGGCATTAAT 
CGCTACAGTT GTGTCTGCTC ACCAGGATTC ACAGGGCAGA GATGTAACAT TGACATTGAT 
GAGTGTGCCT CCAATCCCTG TCGCAAGGGT GCAACATGTA TCAACGGTGT GAATGGTTTC 
CGCTGTATAT GCCCCGAGGG ACCCCATCAC CCCAGCTGCT ACTCACAGGT GAACGAATGC 
CTGAGCAATC CCTGCATCCA TGGAAACTGT ACTGGAGGTC TCAGTGGATA TAAGTGTCTC 
TGTGATGCAG GCTGGGTTGG CATCAACTGT GAAGTGGACA AAAATGAATG CCTTTCGAAT 
CCATGCCAGA ATGGAGGAAC TTGTGACAAT CTGGTGAATG GATACAGGTG TACTTGCAAG 
AAGGGCTTTA AAGGCTATAA CTGCCAGGTG AATATTGATG AATGTGCCTC AAATCCATGC 
CTGAACCAAG GAACCTGCTT TGATGACATA AGTGGCTACA CTTGCCACTG TGTGCTGCCA 
TACACAGGCA AGAATTGTCA GACAGTATTG GCTCCCTGTT CCCCAAACCC TTGTGAGAAT 
GCTGCTGTTT GCAAAGAGTC ACCAAATTTT GAGAGTTATA CTTGCTTGTG TGCTCCTGGC 
TGGCAAGGTC AGCGGTGTAC CATTGACATT GACGAGTGTA TCTCCAAGCC CTGCATGAAC 
CATGGTCTCT GCCATAACAC CCAGGGCAGC TACATGTGTG AATGTCCACC AGGCTTCAGT 
GGTATGGACT GTGAGGAGGA CATTGATGAC TGCCTTGCCA ATCCTTGCCA GAATGGAGGT 
TCCTGTATGG ATGGAGTGAA TACTTTCTCC TGCCTCTGCC TTCCGGGTTT CACTGGGGAT 
AAGTGCCAGA CAGACATGAA TGAGTGTCTG AGTGAACCCT GTAAGAATGG AGGGACCTGC 
TCTGACTACG TCAACAGTTA CACTTGCAAG TGCCAGGCAG GATTTGATGG AGTCCATTGT 
GAGAACAACA TCAATGAGTG CACTGAGAGC TCCTGTTTCA ATGGTGGCAC ATGTGTTGAT 
GGGATTAACT CCTTCTCTTG CTTGTGCCCT GTGGGTTTCA CTGGATCCTT CTGCCTCCAT 
GAGATCAATG AATGCAGCTC TCATCCATGC CTGAATGAGG GAACGTGTGT TGATGGCCTG 
GGTACCTACC GCTGCAGCTG CCCCCTGGGC TACACTGGGA AAAACTGTCA GACCCTGGTG 
AATCTCTGCA GTCGGTCTCC ATGTAAAAAC AAAGGTACTT GCGTTCAGAA AAAAGCAGAG 
TCCCAGTGCC TATGTCCATC TGGATGGGCT GGTGCCTATT GTGACGTGCC CAATGTCTCT 
TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC ACTTGTGCCA GCACTCAGGT 
GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC CCCTGGGCTA TACTGGGAGC 
TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT GCCAGCACGG GGCAACATGC 
AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG GCTATCAGGG TGTCAACTGT 
GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA ATGGAGGCAC CTGTATTGAC 
CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC GGGGCCTACT CTGTGAAGAG 
AACATTGATG ACTGTGCCCG GGGTCCCCAT TGCCTTAATG GTGGTCAGTG CATGGATAGG 
ATTGGAGGCT ACAGTTGTCG CTGCTTGCCT GGCTTTGCTG GGGAGCGTTG TGAGGGAGAC 
ATCAACGAGT GCCTCTCCAA CCCCTGCAGC TCTGAGGGCA GCCTGGACTG TATACAGCTC 
ACCAATGACT ACCTGTGTGT TTGCCGTAGT GCCTTTACTG GCCGGCACTG TGAAACCTTC 
GTCGATGTGT GTCCCCAGAT GCCCTGCCTG AATGGAGGGA CTTGTGCTGT GGCCAGTAAC 
ATGCCTGATG GTTTCATTTG CCGTTGTCCC CCGGGATTTT CCGGGGCAAG GTGCCAGAGC 
AGCTGTGGAC AAGTGAAATG TAGGAAGGGG GAGCAGTGTG TGCACACCGC CTCTGGACCC 
CGCTGCTTCT GCCCCAGTCC CCGGGACTGC GAGTCAGGCT GTGCCAGTAG CCCCTGCCAG 
CACGGGGGCA GCTGCCACCC TCAGCGCCAG CCTCCTTATT ACTCCTGCCA GTGTGCCCCA 
CCATTCTCGG GTAGCCGCTG TGAACTCTAC ACGGCACCCC CCAGCACCCC TCCTGCCACC 
TGTCTGAGCC AGTATTGTGC CGACAAAGCT CGGGATGGCG TCTGTGATGA GGCCTGCAAC 
AGCCATGCCT GCCAGTGGGA TGGGGGTGAC TGTTCTCTCA CCATGGAGAA CCCCTGGGCC 
AACTGCTCCT CCCCACTTCC CTGCTGGGAT TATATCAACA ACCAGTGTGA TGAGCTGTGC 
AACACGGTCG AGTGCCTGTT TGACAACTTT GAATGCCAGG GGAACAGCAA GACATGCAAG 
TATGACAAAT ACTGTGCAGA CCACTTCAAA GACAACCACT GTGACCAGGG GTGCAACAGT 
GAGGAGTGTG GTTGGGATGG GCTGGACTGT GCTGCTGACC AACCTGAGAA CCTGGCAGAA 
GGTACCCTGG TTATTGTGGT ATTGATGCCA CCTGAACAAC TGCTCCAGGA TGCTCGCAGC 
TTCTTGCGGG CACTGGGTAC CCTGCTCCAC ACCAACCTGC GCATTAAGCG GGACTCCCAG 
GGGGAACTCA TGGTGTACCC CTATTATGGT GAGAAGTCAG CTGCTATGAA GAAACAGAGG 
ATGACACGCA GATCCCTTCC TGGTGAACAA GAACAGGAGG TGGCTGGCTC TAAAGTCTTT 
CTGGAAATTG ACAACCGCCA GTGTGTTCAA GACTCAGACC ACTGCTTCAA GAACACGGAT 
GCAGCAGCAG CTCTCCTGGC CTCTCACGCC ATACAGGGGA CCCTGTCATA CCCTCTTGTG 
TCTGTCGTCA GTGAATCCCT GACTCCAGAA CGCACTCAGC TCCTCTATCT CCTTGCTGTT 
GCTGTTGTCA TCATTCTGTT TATTATTCTG CTGGGGGTAA TCATGGCAAA ACGAAAGCGT 
AAGCATGGCT CTCTCTGGCT GCCTGAAGGT TTCACTCTTC GCCGAGATGC AAGCAATCAC 
AAGCGTCGTG AGCCAGTGGG ACAGGATGCT GTGGGGCTGA AAAATCTCTC AGTGCAAGTC 
TCAGAAGCTA ACCTAATTGG TACTGGAACA AGTGAACACT GGGTCGATGA TGAAGGGCCC 
CAGCCAAAGA AAGTAAAGGC TGAAGATGAG GCCTTACTCT CAGAAGAAGA TGACCCCATT 
GATCGACGGC CATGGACACA GCAGCACCTT GAAGCTGCAG ACATCCGTAG GACACCATCG 
CTGGCTCTCA CCCCTCCTCA GGCAGAGCAG GAGGTGGATG TGTTAGATGT GAATGTCCGT 
GGCCCAGATG GCTGCACCCC ATTGATGTTG GCTTCTCTCC GAGGAGGCAG CTCAGATTTG 
AGTGATGAAG ATGAAGATGC AGAGGACTCT TCTGCTAACA TCATCACAGA CTTGGTCTAC 
CAGGGTGCCA GCCTCCAGGC CCAGACAGAC CGGACTGGTG AGATGGCCCT GCACCTTGCA 
GCCCGCTACT CACGGGCTGA TGCTGCCAAG CGTCTCCTGG ATGCAGGTGC AGATGCCAAT 
GCCCAGGACA ACATGGGCCG CTGTCCACTC CATGCTGCAG TGGCAGCTGA TGCCCAAGGT 
GTCTTCCAGA TTCTGATTCG CAACCGAGTA ACTGATCTAG ATGCCAGGAT GAATGATGGT 
ACTACACCCC TGATCCTGGC TGCCCGCCTG GCTGTGGAGG GAATGGTGGC AGAACTGATC 
AACTGCCAAG CGGATGTGAA TGCAGTGGAT GACCATGGAA AATCTGCTCT TCACTGGGCA 
GCTGCTGTCA ATAATGTGGA GGCAACTCTT TTGTTGTTGA AAAATGGGGC CAACCGAGAC 
ATGCAGGACA ACAAGGAAGA GACACCTCTG TTTCTTGCTG CCCGGGAGGG GAGCTATGAA 
GCAGCCAAGA TCCTGTTAGA CCATTTTGCC AATCGAGACA TCACAGACCA TATGGATCGT 
CTTCCCCGGG ATGTGGCTCG GGATCGCATG CACCATGACA TTGTGCGCCT TCTGGATGAA 
TACAATGTGA CCCCAAGCCC TCCAGGCACC GTGTTGACTT CTGCTCTCTC ACCTGTCATC 
TGTGGGCCCA ACAGATCTTT CCTCAGCCTG AAGCACACCC CAATGGGCAA GAAGTCTAGA 
CGGCCCAGTG CCAAGAGTAC CATGCCTACT AGCCTCCCTA ACCTTGCCAA GGAGGCAAAG 
GATGCCAAGG GTAGTAGGAG GAAGAAGTCT CTGAGTGAGA AGGTCCAACT GTCTGAGAGT 
TCAGTAACTT TATCCCCTGT TGATTCCCTA GAATCTCCTC ACACGTATGT TTCCGACACC 
ACATCCTCTC CAATGATTAC ATCCCCTGGG ATCTTACAGG CCTCACCCAA CCCTATGTTG 
GCCACTGCCG CCCCTCCTGC CCCAGTCCAT GCCCAGCATG CACTATCTTT TTCTAACCTT 
CATGAAATGC AGCCTTTGGC ACATGGGGCC AGCACTGTGC TTCCCTCAGT GAGCCAGTTG 
CTATCCCACC ACCACATTGT GTCTCCAGGC AGTGGCAGTG CTGGAAGCTT GAGTAGGCTC 
CATCCAGTCC CAGTCCCAGC AGATTGGATG AACCGCATGG AGGTGAATGA GACCCAGTAC 
AATGAGATGT TTGGTATGGT CCTGGCTCCA GCTGAGGGCA CCCATCCTGG CATAGCTCCC 
CAGAGCAGGC CACCTGAAGG GAAGCACATA ACCACCCCTC GGGAGCCCTT GCCCCCCATT 
GTGACTTTCC AGCTCATCCC TAAAGGCAGT ATTGCCCAAC CAGCGGGGGC TCCCCAGCCT 
CAGTCCACCT GCCCTCCAGC TGTTGCGGGC CCCCTGCCCA CCATGTACCA GATTCCAGAA 
ATGGCCCGTT TGCCCAGTGT GGCTTTCCCC ACTGCCATGA TGCCCCAGCA GGACGGGCAG 
GTAGCTCAGA CCATTCTCCC AGCCTATCAT CCTTTCCCAG CCTCTGTGGG CAAGTACCCC 
ACACCCCCTT CACAGCACAG TTATGCTTCC TCAAATGCTG CTGAGCGAAC ACCCAGTCAC 
AGTGGTCACC TCCAGGGTGA GCATCCCTAC CTGACACCAT CCCCAGAGTC TCCTGACCAG 
TGGTCAAGTT CATCACCCCA CTCTGCTTCT GACTGGTCAG ATGTGACCAC CAGCCCTACC 
CCTGGGGGTG CTGGAGGAGG TCAGCGGGGA CCTGGGACAC ACATGTCTGA GCCACCACAC 
AACAACATGC AGGTTTATGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NOTCH2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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