NOS3 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001160109.1	NP_001153581.1	nitric oxide synthase, endothelial isoform 2
NM_000603.5	NP_000594.2	nitric oxide synthase, endothelial isoform 1
NM_001160111.1	NP_001153583.1	nitric oxide synthase, endothelial isoform 4
NM_001160109.2	NP_001153581.1	nitric oxide synthase, endothelial isoform 2
XM_017012232.1	XP_016867721.1	nitric oxide synthase, endothelial isoform X1
NM_001160110.1	NP_001153582.1	nitric oxide synthase, endothelial isoform 3
XM_006716002.3	XP_006716065.1	nitric oxide synthase, endothelial isoform X4
XM_017012234.1	XP_016867723.1	nitric oxide synthase, endothelial isoform X3
NM_000603.4	NP_000594.2	nitric oxide synthase, endothelial isoform 1
XM_017012233.1	XP_016867722.1	nitric oxide synthase, endothelial isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26678	NM_000603.5 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu27288	NM_001160111.1 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26678	XM_017012232.1 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26968	NM_001160109.2 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27232	NM_001160110.1 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu97268	XM_017012233.1 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu41571	XM_006716002.3 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu97269	XM_017012234.1 Latest version!	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu26968	NM_001160109.1	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu26678	NM_000603.4	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

NOS3 ( NM_000603.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000594.2 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26678
Clone ID Related Accession (Same CDS sequence)	NM_000603.5 , XM_017012232.1 , NM_000603.4
Accession Version	NM_000603.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010973.7, DA859819.1, BC063294.1 and AI082109.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000603.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 18474398). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000297494.8/ ENSP00000297494.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000594.2
CDS	336..3947
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

Target ORF information:

RefSeq Version	NM_000603.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000603.5

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_000603.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000594.2 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26678
Clone ID Related Accession (Same CDS sequence)	NM_000603.5 , XM_017012232.1 , NM_000603.4
Accession Version	NM_000603.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA859819.1, BC063294.1 and AI082109.1. This sequence is a reference standard in the RefSeqGene project. On May 12, 2009 this sequence version replaced NM_000603.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 18474398). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000594.2
CDS	297..3908
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

Target ORF information:

RefSeq Version	NM_000603.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000603.4

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_001160111.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153583.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 4, mRNA.

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CloneID	OHu27288
Clone ID Related Accession (Same CDS sequence)	NM_001160111.1
Accession Version	NM_001160111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ256131.1. Transcript Variant: This variant (4), also known as eNOS13C, uses an alternate 3' exon and poly-adenylation site, compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ256131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2153031 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGGGTCTCAC TTTGTGGCCC AGGCTGGAGT GCAGTAGTAC AATCACGGCT 
CACTGCAGCC TCAACCTCCT AGACTCAAGC AATCCTCCCA CTTCAACCTC CCAAGTAGTT 
GGGACTACAG GCGCATGCCA TGATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153583.1
CDS	1..1890
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGEGLTLWPRLECSSTITAHCSLNLLDSSNPPTSTSQVVGTTGACHDA

Target ORF information:

RefSeq Version	NM_001160111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160111.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGGGTCTCAC TTTGTGGCCC AGGCTGGAGT GCAGTAGTAC AATCACGGCT 
CACTGCAGCC TCAACCTCCT AGACTCAAGC AATCCTCCCA CTTCAACCTC CCAAGTAGTT 
GGGACTACAG GCGCATGCCA TGATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( XM_017012232.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867721.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

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CloneID	OHu26678
Clone ID Related Accession (Same CDS sequence)	NM_000603.5 , XM_017012232.1 , NM_000603.4
Accession Version	XM_017012232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867721.1
CDS	54..3665
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

Target ORF information:

RefSeq Version	XM_017012232.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012232.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGCCGCCTG TGAGACCTTC TGTGTGGGAG AGGATGCCAA GGCCGCCGCC 
CGAGACATCT TCAGCCCCAA ACGGAGCTGG AAGCGCCAGA GGTACCGGCT GAGCGCCCAG 
GCCGAGGGCC TGCAGTTGCT GCCAGGTCTG ATCCACGTGC ACAGGCGGAA GATGTTCCAG 
GCTACAATCC GCTCAGTGGA AAACCTGCAA AGCAGCAAGT CCACGAGGGC CACCATCCTG 
GTGCGCCTGG ACACCGGAGG CCAGGAGGGG CTGCAGTACC AGCCGGGGGA CCACATAGGT 
GTCTGCCCGC CCAACCGGCC CGGCCTTGTG GAGGCGCTGC TGAGCCGCGT GGAGGACCCG 
CCGGCGCCCA CTGAGCCCGT GGCAGTAGAG CAGCTGGAGA AGGGCAGCCC TGGTGGCCCT 
CCCCCCGGCT GGGTGCGGGA CCCCCGGCTG CCCCCGTGCA CGCTGCGCCA GGCTCTCACC 
TTCTTCCTGG ACATCACCTC CCCACCCAGC CCTCAGCTCT TGCGGCTGCT CAGCACCTTG 
GCAGAAGAGC CCAGGGAACA GCAGGAGCTG GAGGCCCTCA GCCAGGATCC CCGACGCTAC 
GAGGAGTGGA AGTGGTTCCG CTGCCCCACG CTGCTGGAGG TGCTGGAGCA GTTCCCGTCG 
GTGGCGCTGC CTGCCCCACT GCTCCTCACC CAGCTGCCTC TGCTCCAGCC CCGGTACTAC 
TCAGTCAGCT CGGCACCCAG CACCCACCCA GGAGAGATCC ACCTCACTGT AGCTGTGCTG 
GCATACAGGA CTCAGGATGG GCTGGGCCCC CTGCACTATG GAGTCTGCTC CACGTGGCTA 
AGCCAGCTCA AGCCCGGAGA CCCTGTGCCC TGCTTCATCC GGGGGGCTCC CTCCTTCCGG 
CTGCCACCCG ATCCCAGCTT GCCCTGCATC CTGGTGGGTC CAGGCACTGG CATTGCCCCC 
TTCCGGGGAT TCTGGCAGGA GCGGCTGCAT GACATTGAGA GCAAAGGGCT GCAGCCCACT 
CCCATGACTT TGGTGTTCGG CTGCCGATGC TCCCAACTTG ACCATCTCTA CCGCGACGAG 
GTGCAGAACG CCCAGCAGCG CGGGGTGTTT GGCCGAGTCC TCACCGCCTT CTCCCGGGAA 
CCTGACAACC CCAAGACCTA CGTGCAGGAC ATCCTGAGGA CGGAGCTGGC TGCGGAGGTG 
CACCGCGTGC TGTGCCTCGA GCGGGGCCAC ATGTTTGTCT GCGGCGATGT TACCATGGCA 
ACCAACGTCC TGCAGACCGT GCAGCGCATC CTGGCGACGG AGGGCGACAT GGAGCTGGAC 
GAGGCCGGCG ACGTCATCGG CGTGCTGCGG GATCAGCAAC GCTACCACGA AGACATTTTC 
GGGCTCACGC TGCGCACCCA GGAGGTGACA AGCCGCATAC GCACCCAGAG CTTTTCCTTG 
CAGGAGCGTC AGTTGCGGGG CGCAGTGCCC TGGGCGTTCG ACCCTCCCGG CTCAGACACC 
AACAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_001160109.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153581.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.

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CloneID	OHu26968
Clone ID Related Accession (Same CDS sequence)	NM_001160109.1 , NM_001160109.2
Accession Version	NM_001160109.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ256129.1 and BC063294.1. Transcript Variant: This variant (2), also known as eNOS13A, uses an alternate 3' exon and poly-adenylation site, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ256129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGTCTGTCTC CCTGCCAGAA GTGTCTGTCA CCACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153581.1
CDS	1..1791
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESVSLPEVSVTTE

Target ORF information:

RefSeq Version	NM_001160109.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160109.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGTCTGTCTC CCTGCCAGAA GTGTCTGTCA CCACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_001160109.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153581.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26968
Clone ID Related Accession (Same CDS sequence)	NM_001160109.1 , NM_001160109.2
Accession Version	NM_001160109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010973.7, DQ256129.1 and BC063294.1. On May 31, 2019 this sequence version replaced NM_001160109.1. Transcript Variant: This variant (2), also known as eNOS13A, uses an alternate 3' exon and poly-adenylation site, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ256129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGTCTGTCTC CCTGCCAGAA GTGTCTGTCA CCACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153581.1
CDS	23..1813
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESVSLPEVSVTTE

Target ORF information:

RefSeq Version	NM_001160109.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160109.2

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGTCTGTCTC CCTGCCAGAA GTGTCTGTCA CCACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_001160110.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153582.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 3, mRNA.

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CloneID	OHu27232
Clone ID Related Accession (Same CDS sequence)	NM_001160110.1
Accession Version	NM_001160110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ256130.1 and BC063294.1. Transcript Variant: This variant (3), also known as eNOS13B, uses an alternate 3' exon and poly-adenylation site, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ256130.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGATGGGG TTTCGCCATG TTGCCCAGGC TGGTCTCTAA CTCCTGGGTT 
CAAGCAATCC ACCTGCCTCG GCCTCCCAAA GTGCTGCGAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153582.1
CDS	1..1845
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGERWGFAMLPRLVSNSWVQAIHLPRPPKVLRL

Target ORF information:

RefSeq Version	NM_001160110.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160110.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGATGGGG TTTCGCCATG TTGCCCAGGC TGGTCTCTAA CTCCTGGGTT 
CAAGCAATCC ACCTGCCTCG GCCTCCCAAA GTGCTGCGAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( XM_017012233.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867722.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA.

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CloneID	OHu97268
Clone ID Related Accession (Same CDS sequence)	XM_017012233.1
Accession Version	XM_017012233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCT ACATCTGCAA CCACATCAAG TATGCCACCA ACCGGGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCTGCCCT GGCCGAGGAG ACTTCCGAAT CTGGAACAGC 
CAGCTGGTGC GCTACGCGGG CTACCGGCAG CAGGATGGCT CTGTGCGGGG GGACCCAGCC 
AACGTGGAGA TCACCGAGCT CTGCATTCAG CACGGCTGGA CCCCAGGAAA CGGTCGCTTC 
GACGTGCTGC CCCTGCTGCT GCAGGCCCCA GATGATCCCC CAGAACTCTT CCTTCTGCCC 
CCCGAGCTGG TCCTTGAGGT GCCCCTGGAG CACCCCACGC TGGAGTGGTT TGCAGCCCTG 
GGCCTGCGCT GGTACGCCCT CCCGGCAGTG TCCAACATGC TGCTGGAAAT TGGGGGCCTG 
GAGTTCCCCG CAGCCCCCTT CAGTGGCTGG TACATGAGCA CTGAGATCGG CACGAGGAAC 
CTGTGTGACC CTCACCGCTA CAACATCCTG GAGGATGTGG CTGTCTGCAT GGACCTGGAT 
ACCCGGACCA CCTCGTCCCT GTGGAAAGAC AAGGCAGCAG TGGAAATCAA CGTGGCCGTG 
CTGCACAGTT ACCAGCTAGC CAAAGTCACC ATCGTGGACC ACCACGCCGC CACGGCCTCT 
TTCATGAAGC ACCTGGAGAA TGAGCAGAAG GCCAGGGGGG GCTGCCCTGC AGACTGGGCC 
TGGATCGTGC CCCCCATCTC GGGCAGCCTC ACTCCTGTTT TCCATCAGGA GATGGTCAAC 
TATTTCCTGT CCCCGGCCTT CCGCTACCAG CCAGACCCCT GGAAGGGGAG TGCCGCCAAG 
GGCACCGGCA TCACCAGGAA GAAGACCTTT AAAGAAGTGG CCAACGCCGT GAAGATCTCC 
GCCTCGCTCA TGGGCACGGT GATGGCGAAG CGAGTGAAGG CGACAATCCT GTATGGCTCC 
GAGACCGGCC GGGCCCAGAG CTACGCACAG CAGCTGGGGA GACTCTTCCG GAAGGCTTTT 
GATCCCCGGG TCCTGTGTAT GGATGAGTAT GACGTGGTGT CCCTCGAACA CGAGACGCTG 
GTGCTGGTGG TAACCAGCAC ATTTGGGAAT GGGGATCCCC CGGAGAATGG AGAGAGCTTT 
GCAGCTGCCC TGATGGAGAT GTCCGGCCCC TACAACAGCT CCCCTCGGCC GGAACAGCAC 
AAGAGTTATA AGATCCGCTT CAACAGCATC TCCTGCTCAG ACCCACTGGT GTCCTCTTGG 
CGGCGGAAGA GGAAGGAGTC CAGTAACACA GACAGTGCAG GGGCCCTGGG CACCCTCAGG 
TTCTGTGTGT TCGGGCTCGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT TGCTCGTGCC 
GTGGACACAC GGCTGGAGGA ACTGGGCGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAC 
GAGCTGTGCG GCCAGGAGGA GGCCTTCCGA GGCTGGGCCC AGGCTGCCTT CCAGGCCGCC 
TGTGAGACCT TCTGTGTGGG AGAGGATGCC AAGGCCGCCG CCCGAGACAT CTTCAGCCCC 
AAACGGAGCT GGAAGCGCCA GAGGTACCGG CTGAGCGCCC AGGCCGAGGG CCTGCAGTTG 
CTGCCAGGTC TGATCCACGT GCACAGGCGG AAGATGTTCC AGGCTACAAT CCGCTCAGTG 
GAAAACCTGC AAAGCAGCAA GTCCACGAGG GCCACCATCC TGGTGCGCCT GGACACCGGA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GTGTCTGCCC GCCCAACCGG 
CCCGGCCTTG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCGGCGCC CACTGAGCCC 
GTGGCAGTAG AGCAGCTGGA GAAGGGCAGC CCTGGTGGCC CTCCCCCCGG CTGGGTGCGG 
GACCCCCGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCTCAGCT CTTGCGGCTG CTCAGCACCT TGGCAGAAGA GCCCAGGGAA 
CAGCAGGAGC TGGAGGCCCT CAGCCAGGAT CCCCGACGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CGGTGGCGCT GCCTGCCCCA 
CTGCTCCTCA CCCAGCTGCC TCTGCTCCAG CCCCGGTACT ACTCAGTCAG CTCGGCACCC 
AGCACCCACC CAGGAGAGAT CCACCTCACT GTAGCTGTGC TGGCATACAG GACTCAGGAT 
GGGCTGGGCC CCCTGCACTA TGGAGTCTGC TCCACGTGGC TAAGCCAGCT CAAGCCCGGA 
GACCCTGTGC CCTGCTTCAT CCGGGGGGCT CCCTCCTTCC GGCTGCCACC CGATCCCAGC 
TTGCCCTGCA TCCTGGTGGG TCCAGGCACT GGCATTGCCC CCTTCCGGGG ATTCTGGCAG 
GAGCGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCA CTCCCATGAC TTTGGTGTTC 
GGCTGCCGAT GCTCCCAACT TGACCATCTC TACCGCGACG AGGTGCAGAA CGCCCAGCAG 
CGCGGGGTGT TTGGCCGAGT CCTCACCGCC TTCTCCCGGG AACCTGACAA CCCCAAGACC 
TACGTGCAGG ACATCCTGAG GACGGAGCTG GCTGCGGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTTACCATGG CAACCAACGT CCTGCAGACC 
GTGCAGCGCA TCCTGGCGAC GGAGGGCGAC ATGGAGCTGG ACGAGGCCGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTACCAC GAAGACATTT TCGGGCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGCAT ACGCACCCAG AGCTTTTCCT TGCAGGAGCG TCAGTTGCGG 
GGCGCAGTGC CCTGGGCGTT CGACCCTCCC GGCTCAGACA CCAACAGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867722.1
CDS	571..3564
Translation	MFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

Target ORF information:

RefSeq Version	XM_017012233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012233.1

ORF Insert Sequence:

ATGTTCACCT ACATCTGCAA CCACATCAAG TATGCCACCA ACCGGGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCTGCCCT GGCCGAGGAG ACTTCCGAAT CTGGAACAGC 
CAGCTGGTGC GCTACGCGGG CTACCGGCAG CAGGATGGCT CTGTGCGGGG GGACCCAGCC 
AACGTGGAGA TCACCGAGCT CTGCATTCAG CACGGCTGGA CCCCAGGAAA CGGTCGCTTC 
GACGTGCTGC CCCTGCTGCT GCAGGCCCCA GATGATCCCC CAGAACTCTT CCTTCTGCCC 
CCCGAGCTGG TCCTTGAGGT GCCCCTGGAG CACCCCACGC TGGAGTGGTT TGCAGCCCTG 
GGCCTGCGCT GGTACGCCCT CCCGGCAGTG TCCAACATGC TGCTGGAAAT TGGGGGCCTG 
GAGTTCCCCG CAGCCCCCTT CAGTGGCTGG TACATGAGCA CTGAGATCGG CACGAGGAAC 
CTGTGTGACC CTCACCGCTA CAACATCCTG GAGGATGTGG CTGTCTGCAT GGACCTGGAT 
ACCCGGACCA CCTCGTCCCT GTGGAAAGAC AAGGCAGCAG TGGAAATCAA CGTGGCCGTG 
CTGCACAGTT ACCAGCTAGC CAAAGTCACC ATCGTGGACC ACCACGCCGC CACGGCCTCT 
TTCATGAAGC ACCTGGAGAA TGAGCAGAAG GCCAGGGGGG GCTGCCCTGC AGACTGGGCC 
TGGATCGTGC CCCCCATCTC GGGCAGCCTC ACTCCTGTTT TCCATCAGGA GATGGTCAAC 
TATTTCCTGT CCCCGGCCTT CCGCTACCAG CCAGACCCCT GGAAGGGGAG TGCCGCCAAG 
GGCACCGGCA TCACCAGGAA GAAGACCTTT AAAGAAGTGG CCAACGCCGT GAAGATCTCC 
GCCTCGCTCA TGGGCACGGT GATGGCGAAG CGAGTGAAGG CGACAATCCT GTATGGCTCC 
GAGACCGGCC GGGCCCAGAG CTACGCACAG CAGCTGGGGA GACTCTTCCG GAAGGCTTTT 
GATCCCCGGG TCCTGTGTAT GGATGAGTAT GACGTGGTGT CCCTCGAACA CGAGACGCTG 
GTGCTGGTGG TAACCAGCAC ATTTGGGAAT GGGGATCCCC CGGAGAATGG AGAGAGCTTT 
GCAGCTGCCC TGATGGAGAT GTCCGGCCCC TACAACAGCT CCCCTCGGCC GGAACAGCAC 
AAGAGTTATA AGATCCGCTT CAACAGCATC TCCTGCTCAG ACCCACTGGT GTCCTCTTGG 
CGGCGGAAGA GGAAGGAGTC CAGTAACACA GACAGTGCAG GGGCCCTGGG CACCCTCAGG 
TTCTGTGTGT TCGGGCTCGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT TGCTCGTGCC 
GTGGACACAC GGCTGGAGGA ACTGGGCGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAC 
GAGCTGTGCG GCCAGGAGGA GGCCTTCCGA GGCTGGGCCC AGGCTGCCTT CCAGGCCGCC 
TGTGAGACCT TCTGTGTGGG AGAGGATGCC AAGGCCGCCG CCCGAGACAT CTTCAGCCCC 
AAACGGAGCT GGAAGCGCCA GAGGTACCGG CTGAGCGCCC AGGCCGAGGG CCTGCAGTTG 
CTGCCAGGTC TGATCCACGT GCACAGGCGG AAGATGTTCC AGGCTACAAT CCGCTCAGTG 
GAAAACCTGC AAAGCAGCAA GTCCACGAGG GCCACCATCC TGGTGCGCCT GGACACCGGA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GTGTCTGCCC GCCCAACCGG 
CCCGGCCTTG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCGGCGCC CACTGAGCCC 
GTGGCAGTAG AGCAGCTGGA GAAGGGCAGC CCTGGTGGCC CTCCCCCCGG CTGGGTGCGG 
GACCCCCGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCTCAGCT CTTGCGGCTG CTCAGCACCT TGGCAGAAGA GCCCAGGGAA 
CAGCAGGAGC TGGAGGCCCT CAGCCAGGAT CCCCGACGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CGGTGGCGCT GCCTGCCCCA 
CTGCTCCTCA CCCAGCTGCC TCTGCTCCAG CCCCGGTACT ACTCAGTCAG CTCGGCACCC 
AGCACCCACC CAGGAGAGAT CCACCTCACT GTAGCTGTGC TGGCATACAG GACTCAGGAT 
GGGCTGGGCC CCCTGCACTA TGGAGTCTGC TCCACGTGGC TAAGCCAGCT CAAGCCCGGA 
GACCCTGTGC CCTGCTTCAT CCGGGGGGCT CCCTCCTTCC GGCTGCCACC CGATCCCAGC 
TTGCCCTGCA TCCTGGTGGG TCCAGGCACT GGCATTGCCC CCTTCCGGGG ATTCTGGCAG 
GAGCGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCA CTCCCATGAC TTTGGTGTTC 
GGCTGCCGAT GCTCCCAACT TGACCATCTC TACCGCGACG AGGTGCAGAA CGCCCAGCAG 
CGCGGGGTGT TTGGCCGAGT CCTCACCGCC TTCTCCCGGG AACCTGACAA CCCCAAGACC 
TACGTGCAGG ACATCCTGAG GACGGAGCTG GCTGCGGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTTACCATGG CAACCAACGT CCTGCAGACC 
GTGCAGCGCA TCCTGGCGAC GGAGGGCGAC ATGGAGCTGG ACGAGGCCGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTACCAC GAAGACATTT TCGGGCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGCAT ACGCACCCAG AGCTTTTCCT TGCAGGAGCG TCAGTTGCGG 
GGCGCAGTGC CCTGGGCGTT CGACCCTCCC GGCTCAGACA CCAACAGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( XM_006716002.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716065.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu41571
Clone ID Related Accession (Same CDS sequence)	XM_006716002.3
Accession Version	XM_006716002.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716002.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716065.1
CDS	54..2171
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQV

Target ORF information:

RefSeq Version	XM_006716002.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716002.3

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( XM_017012234.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867723.1 Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA.

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CloneID	OHu97269
Clone ID Related Accession (Same CDS sequence)	XM_017012234.1
Accession Version	XM_017012234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, endothelial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGAACACAAT GAGGTGTAAA GATGGGGAAA CTGAGGCATG TCACTGTAAG 
TACGGGATTC GGAATTTGAA TGCAGGTCTG AACACACAGA CGCCTTCACA GAGCTACCGT 
GTGCCAAGCA CTATGCTTCT CGGATCACGG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867723.1
CDS	54..2309
Translation	MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQNTMRCKDGETEACHCKYGIRNLNAGLNTQTPSQSYRVPSTMLLGSRD

Target ORF information:

RefSeq Version	XM_017012234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012234.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGCGT GGCCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTTG GGCTGTGCGG CAAGCAGGGC CCAGCCACCC CGGCCCCTGA GCCCAGCCGG 
GCCCCAGCAT CCCTACTCCC ACCAGCGCCA GAACACAGCC CCCCGAGCTC CCCGCTAACC 
CAGCCCCCAG AGGGGCCCAA GTTCCCTCGT GTGAAGAACT GGGAGGTGGG GAGCATCACC 
TATGACACCC TCAGCGCCCA GGCGCAGCAG GATGGGCCCT GCACCCCAAG ACGCTGCCTG 
GGCTCCCTGG TATTTCCACG GAAACTACAG GGCCGGCCCT CCCCCGGCCC CCCGGCCCCT 
GAGCAGCTGC TGAGTCAGGC CCGGGACTTC ATCAACCAGT ACTACAGCTC CATTAAGAGG 
AGCGGCTCCC AGGCCCACGA ACAGCGGCTT CAAGAGGTGG AAGCCGAGGT GGCAGCCACA 
GGCACCTACC AGCTTAGGGA GAGCGAGCTG GTGTTCGGGG CTAAGCAGGC CTGGCGCAAC 
GCTCCCCGCT GCGTGGGCCG GATCCAGTGG GGGAAGCTGC AGGTGTTCGA TGCCCGGGAC 
TGCAGGTCTG CACAGGAAAT GTTCACCTAC ATCTGCAACC ACATCAAGTA TGCCACCAAC 
CGGGGCAACC TTCGCTCGGC CATCACAGTG TTCCCGCAGC GCTGCCCTGG CCGAGGAGAC 
TTCCGAATCT GGAACAGCCA GCTGGTGCGC TACGCGGGCT ACCGGCAGCA GGATGGCTCT 
GTGCGGGGGG ACCCAGCCAA CGTGGAGATC ACCGAGCTCT GCATTCAGCA CGGCTGGACC 
CCAGGAAACG GTCGCTTCGA CGTGCTGCCC CTGCTGCTGC AGGCCCCAGA TGATCCCCCA 
GAACTCTTCC TTCTGCCCCC CGAGCTGGTC CTTGAGGTGC CCCTGGAGCA CCCCACGCTG 
GAGTGGTTTG CAGCCCTGGG CCTGCGCTGG TACGCCCTCC CGGCAGTGTC CAACATGCTG 
CTGGAAATTG GGGGCCTGGA GTTCCCCGCA GCCCCCTTCA GTGGCTGGTA CATGAGCACT 
GAGATCGGCA CGAGGAACCT GTGTGACCCT CACCGCTACA ACATCCTGGA GGATGTGGCT 
GTCTGCATGG ACCTGGATAC CCGGACCACC TCGTCCCTGT GGAAAGACAA GGCAGCAGTG 
GAAATCAACG TGGCCGTGCT GCACAGTTAC CAGCTAGCCA AAGTCACCAT CGTGGACCAC 
CACGCCGCCA CGGCCTCTTT CATGAAGCAC CTGGAGAATG AGCAGAAGGC CAGGGGGGGC 
TGCCCTGCAG ACTGGGCCTG GATCGTGCCC CCCATCTCGG GCAGCCTCAC TCCTGTTTTC 
CATCAGGAGA TGGTCAACTA TTTCCTGTCC CCGGCCTTCC GCTACCAGCC AGACCCCTGG 
AAGGGGAGTG CCGCCAAGGG CACCGGCATC ACCAGGAAGA AGACCTTTAA AGAAGTGGCC 
AACGCCGTGA AGATCTCCGC CTCGCTCATG GGCACGGTGA TGGCGAAGCG AGTGAAGGCG 
ACAATCCTGT ATGGCTCCGA GACCGGCCGG GCCCAGAGCT ACGCACAGCA GCTGGGGAGA 
CTCTTCCGGA AGGCTTTTGA TCCCCGGGTC CTGTGTATGG ATGAGTATGA CGTGGTGTCC 
CTCGAACACG AGACGCTGGT GCTGGTGGTA ACCAGCACAT TTGGGAATGG GGATCCCCCG 
GAGAATGGAG AGAGCTTTGC AGCTGCCCTG ATGGAGATGT CCGGCCCCTA CAACAGCTCC 
CCTCGGCCGG AACAGCACAA GAGTTATAAG ATCCGCTTCA ACAGCATCTC CTGCTCAGAC 
CCACTGGTGT CCTCTTGGCG GCGGAAGAGG AAGGAGTCCA GTAACACAGA CAGTGCAGGG 
GCCCTGGGCA CCCTCAGGTT CTGTGTGTTC GGGCTCGGCT CCCGGGCATA CCCCCACTTC 
TGCGCCTTTG CTCGTGCCGT GGACACACGG CTGGAGGAAC TGGGCGGGGA GCGGCTGCTG 
CAGCTGGGCC AGGGCGACGA GCTGTGCGGC CAGGAGGAGG CCTTCCGAGG CTGGGCCCAG 
GCTGCCTTCC AGAACACAAT GAGGTGTAAA GATGGGGAAA CTGAGGCATG TCACTGTAAG 
TACGGGATTC GGAATTTGAA TGCAGGTCTG AACACACAGA CGCCTTCACA GAGCTACCGT 
GTGCCAAGCA CTATGCTTCT CGGATCACGG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NOS3
Entrez Gene ID	4846
Full Name	nitric oxide synthase 3
Synonyms	ECNOS,eNOS
General protein information	Preferred Names nitric oxide synthase 3 Names nitric oxide synthase, endothelial EC-NOS NOS type III NOSIII cNOS constitutive NOS endothelial NOS nitric oxide synthase 3 (endothelial cell) nitric oxide synthase 3 transcript variant eNOS-delta20 nitric oxide synthase 3 transcript variant eNOS-delta20-21 nitric oxide synthase 3 transcript variant eNOS-delta21
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q36.1
Summary	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016].
Disorder MIM:	163729
Disorder Html:	{Coronary artery spasm 1, susceptibility to} (3); {Alzheimer disease, late-onset, susceptibility to}, 104300 (3); {Hypertension, susceptibility to}, 145500 (3); {Hypertension, pregnancy-induced}, 189800 (3); {Placental abruption} (3); {Ischemic stroke, susceptibility to}, 601367 (3)

Pan troglodytes (chimpanzee)	NOS3	XP_003318952.1
	NOS3	XP_002803570.1
Xenopus tropicalis (tropical clawed frog)	nos3	NP_001243158.1
Bos taurus (cattle)	NOS3	NP_851380.2
Homo sapiens (human)	NOS3	NP_000594.2
Mus musculus (house mouse)	Nos3	NP_032739.3
Canis lupus familiaris (dog)	NOS3	NP_001003158.2
Rattus norvegicus (Norway rat)	Nos3	NP_068610.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NOS3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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