NMT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NMT1
Entrez Gene ID	4836
Full Name	N-myristoyltransferase 1
Synonyms	NMT
General protein information	Preferred Names N-myristoyltransferase 1 Names glycylpeptide N-tetradecanoyltransferase 1 alternative, short form NMT-S long form, NMT-L myristoyl-CoA:protein N-myristoyltransferase 1 type I N-myristoyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008].
Disorder MIM:	160993

mRNA and Protein(s)

mRNA	Protein	Name
XM_011524858.2	XP_011523160.1	glycylpeptide N-tetradecanoyltransferase 1 isoform X1
NM_021079.5	NP_066565.1	glycylpeptide N-tetradecanoyltransferase 1
NM_021079.4	NP_066565.1	glycylpeptide N-tetradecanoyltransferase 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nmt1	NP_683689.1
Drosophila melanogaster (fruit fly)	Nmt	NP_523969.1
Xenopus tropicalis (tropical clawed frog)	nmt2	XP_002935567.1
Pan troglodytes (chimpanzee)	NMT1	XP_001143871.1
	NMT1	XP_001115168.1
Mus musculus (house mouse)	Nmt1	NP_032733.1
Gallus gallus (chicken)	NMT1	XP_418088.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001804	XP_321256.5
Arabidopsis thaliana (thale cress)	NMT1	NP_568846.1
Danio rerio (zebrafish)	nmt1a	NP_001018316.1
Homo sapiens (human)	NMT1	NP_066565.1
Canis lupus familiaris (dog)	NMT1	XP_537613.2
Bos taurus (cattle)	NMT1	NP_803470.2
Oryza sativa (rice)	Os01g0708100	NP_001044024.1

Gene Ontology
Bibliography

Related articles in PubMed

Blocking Myristoylation of Src Inhibits Its Kinase Activity and Suppresses Prostate Cancer Progression.
Kim S, Alsaidan OA, Goodwin O, Li Q, Sulejmani E, Han Z, Bai A, Albers T, Beharry Z, Zheng YG, Norris JS, Szulc ZM, Bielawska A, Lebedyeva I, Pegan SD, Cai H
Cancer research77(24)6950-6962(2017 Dec)

Association of NMT2 with the acyl-CoA carrier ACBD6 protects the N-myristoyltransferase reaction from palmitoyl-CoA.
Soupene E, Kao J, Cheng DH, Wang D, Greninger AL, Knudsen GM, DeRisi JL, Kuypers FA
Journal of lipid research57(2)288-98(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic and pharmacologic evidence that inhibiting the N-myristoyltransferase NMT1 suppresses cell-cycle progression, proliferation, and malignant growth of prostate cancer cells.
Title: Blocking Myristoylation of Src Inhibits Its Kinase Activity and Suppresses Prostate Cancer Progression.

These observations point at a previously unrecognized contribution of calnexin to the retention of NMT1 at the ER membrane.
Title: N-Myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum.

findings suggest that both NMT1 and hnRNP A2/B1 take part in the regulation of HIV-1 RNA expression through their mutual opposite effects on the viral RNA expression in HIV-1-producing cells
Title: N-Myristoyltransferase 1 enhances human immunodeficiency virus replication through regulation of viral RNA expression level.

we propose a model of the Nef:NMT complex in which only the myristoyl moiety holds the two proteins together in complex and speculate that perhaps NMT chaperones Nef to the membrane and thereby protects the myristic acid group from the cytosol
Title: Effects of HIV-1 Nef on human N-myristoyltransferase 1.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following NMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu59286	NM_021079.5 Latest version!	Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu59285	XM_011524858.2 Latest version!	Homo sapiens N-myristoyltransferase 1 (NMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu59286	NM_021079.4	Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NMT1 ( NM_021079.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_066565.1 Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.

Price & Availability↑

CloneID	OHu59286
Clone ID Related Accession (Same CDS sequence)	NM_021079.5 , NM_021079.4
Accession Version	NM_021079.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	glycylpeptide N-tetradecanoyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC006569.2, AC116493.10, AC002117.2 and BC006538.2. On Nov 22, 2018 this sequence version replaced NM_021079.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006538.2, SRR1660805.232373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000258960.7/ ENSP00000258960.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGAAAGGGTT TGATGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT TGGCATAGGG GACGGCAACC TGCAGTATTA CCTTTACAAT 
TGGAAATGCC CCAGCATGGG GGCAGAGAAG GTTGGACTGG TGCTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066565.1
CDS	14..1504
Translation	MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLVLQ

Target ORF information:

RefSeq Version	NM_021079.5
Organism	Homo sapiens(human)
Definition	Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021079.5

ORF Insert Sequence:

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGAAAGGGTT TGATGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT TGGCATAGGG GACGGCAACC TGCAGTATTA CCTTTACAAT 
TGGAAATGCC CCAGCATGGG GGCAGAGAAG GTTGGACTGG TGCTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMT1 ( NM_021079.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066565.1 Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.

Price & Availability↑

CloneID	OHu59286
Clone ID Related Accession (Same CDS sequence)	NM_021079.5 , NM_021079.4
Accession Version	NM_021079.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	glycylpeptide N-tetradecanoyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU849351.1, BC006569.2, AC116493.10, AC002117.2 and BC006538.2. On Dec 25, 2014 this sequence version replaced NM_021079.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006538.2, SRR1660805.232373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGAAAGGGTT TGATGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT TGGCATAGGG GACGGCAACC TGCAGTATTA CCTTTACAAT 
TGGAAATGCC CCAGCATGGG GGCAGAGAAG GTTGGACTGG TGCTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066565.1
CDS	32..1522
Translation	MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLVLQ

Target ORF information:

RefSeq Version	NM_021079.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-myristoyltransferase 1 (NMT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021079.4

ORF Insert Sequence:

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGAAAGGGTT TGATGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT TGGCATAGGG GACGGCAACC TGCAGTATTA CCTTTACAAT 
TGGAAATGCC CCAGCATGGG GGCAGAGAAG GTTGGACTGG TGCTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMT1 ( XM_011524858.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523160.1 Homo sapiens N-myristoyltransferase 1 (NMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59285
Clone ID Related Accession (Same CDS sequence)	XM_011524858.2
Accession Version	XM_011524858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glycylpeptide N-tetradecanoyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGGTGAGGAG CAGACGGGGG GGTCTCTGGA GATGTGCAGG GAAGAGGCAG 
TGGAGCCATG GTGAGCACAG CTCCCGGGAC GCAGCCTCCC ATGGGCTGGA GGCTCTGAGC 
CCTCCTCATC TGTTCTGCTT AGGCAGGGTT CCCGCAGTTT TTAGGCAGAA ACTCAAAACT 
TGGAGGGAAC AAGGAGCATC CGAAGTGAAG GCATTGAACT CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523160.1
CDS	40..1587
Translation	MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMVRSRRGGLWRCAGKRQWSHGEHSSRDAASHGLEALSPPHLFCLGRVPAVFRQKLKTWREQGASEVKALNSS

Target ORF information:

RefSeq Version	XM_011524858.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-myristoyltransferase 1 (NMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524858.2

ORF Insert Sequence:

ATGGCGGACG AGAGTGAGAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAACG GGAACGGCCA TGAGCACTGC AGCGATTGCG AGAATGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAAAA AGGCAGTGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCCATTGA GCTGTTCTCA 
GTGGGTCAGG GACCTGCCAA AACCATGGAG GAGGCTAGCA AGCGAAGCTA CCAGTTCTGG 
GATACGCAGC CCGTCCCCAA GCTGGGCGAA GTGGTGAACA CCCATGGCCC CGTGGAGCCT 
GACAAGGACA ATATCCGCCA GGAGCCCTAC ACCCTGCCCC AGGGCTTCAC CTGGGATGCT 
TTGGACCTGG GCGATCGTGG TGTGCTAAAA GAACTGTACA CCCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGACAACAT GTTCCGATTT GATTATTCCC CGGAGTTTCT TTTGTGGGCT 
CTCCGGCCAC CCGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAATTGGTTG GGTTCATTAG CGCCATCCCA GCAAACATCC ATATCTATGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGTTCCAA GAGGGTTGCT 
CCAGTTCTGA TCCGAGAGAT CACCAGGCGG GTTCACCTGG AGGGCATCTT CCAAGCAGTT 
TACACTGCCG GGGTGGTACT ACCAAAGCCC GTTGGCACCT GCAGGTATTG GCATCGGTCC 
CTAAACCCAC GGAAGCTGAT TGAAGTGAAG TTCTCCCACC TGAGCAGAAA TATGACCATG 
CAGCGCACCA TGAAGCTCTA CCGACTGCCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAAACAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA CCAGGTACTT GAAGCAATTT 
CACCTTACGC CCGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATCGACA CTTTCGTGGT GGAGAACGCA AACGGAGAGG TGACAGATTT CCTGAGCTTT 
TATACGCTGC CCTCCACCAT CATGAACCAT CCAACCCACA AGAGTCTCAA AGCTGCTTAT 
TCTTTCTACA ACGTTCACAC CCAGACCCCT CTTCTAGACC TCATGAGCGA CGCCCTTGTC 
CTCGCCAAAA TGGTGAGGAG CAGACGGGGG GGTCTCTGGA GATGTGCAGG GAAGAGGCAG 
TGGAGCCATG GTGAGCACAG CTCCCGGGAC GCAGCCTCCC ATGGGCTGGA GGCTCTGAGC 
CCTCCTCATC TGTTCTGCTT AGGCAGGGTT CCCGCAGTTT TTAGGCAGAA ACTCAAAACT 
TGGAGGGAAC AAGGAGCATC CGAAGTGAAG GCATTGAACT CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Blocking Myristoylation of Src Inhibits Its Kinase Activity and Suppresses Prostate Cancer Progression.
Cancer research77(24)6950-6962(2017 Dec)
Kim S,Alsaidan OA,Goodwin O,Li Q,Sulejmani E,Han Z,Bai A,Albers T,Beharry Z,Zheng YG,Norris JS,Szulc ZM,Bielawska A,Lebedyeva I,Pegan SD,Cai H

Association of NMT2 with the acyl-CoA carrier ACBD6 protects the N-myristoyltransferase reaction from palmitoyl-CoA.
Journal of lipid research57(2)288-98(2016 Feb)
Soupene E,Kao J,Cheng DH,Wang D,Greninger AL,Knudsen GM,DeRisi JL,Kuypers FA

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NMT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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