NIT1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005245215.4	XP_005245272.1	nitrilase homolog 1 isoform X1
NM_005600.2	NP_005591.1	deaminated glutathione amidase isoform 1
NM_001185094.1	NP_001172023.1	deaminated glutathione amidase isoform 4
XM_005245214.2	XP_005245271.1	deaminated glutathione amidase isoform X1
XM_017001371.1	XP_016856860.1	deaminated glutathione amidase isoform X5
NM_001185092.1	NP_001172021.1	deaminated glutathione amidase isoform 2
NM_001185092.2	NP_001172021.1	deaminated glutathione amidase isoform 2
NM_005600.3	NP_005591.1	deaminated glutathione amidase isoform 1
XM_024447363.1	XP_024303131.1	deaminated glutathione amidase isoform X4
XM_005245216.4	XP_005245273.1	deaminated glutathione amidase isoform X3
XM_005245216.3	XP_005245273.1	nitrilase homolog 1 isoform X3
XM_006711341.3	XP_006711404.1	nitrilase homolog 1 isoform X4
NM_001185093.1	NP_001172022.1	deaminated glutathione amidase isoform 3
XM_005245215.5	XP_005245272.1	deaminated glutathione amidase isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01275	NM_001185092.2 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu01670	NM_001185094.1 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu05088	NM_005600.3 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu07629	NM_001185093.1 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu01670	XM_005245216.4 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu34413	XM_005245215.5 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu34414	XM_005245214.2 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu34415	XM_006711341.3 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu105053	XM_024447363.1 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu90063	XM_017001371.1 Latest version!	Homo sapiens nitrilase 1 (NIT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu01275	NM_001185092.1	Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu05088	NM_005600.2	Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu01670	XM_005245216.3	Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu34413	XM_005245215.4	Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

NIT1 ( NM_001185092.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172021.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01275
Clone ID Related Accession (Same CDS sequence)	NM_001185092.1 , NM_001185092.2
Accession Version	NM_001185092.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB111935.1, BC046149.1, AL591806.16 and AI277603.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC046149.1, SRR1803612.89339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGCCC 
GTCTCCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172021.1
CDS	106..837
Translation	MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Target ORF information:

RefSeq Version	NM_001185092.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185092.1

ORF Insert Sequence:

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGCCC 
GTCTCCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( NM_001185092.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172021.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01275
Clone ID Related Accession (Same CDS sequence)	NM_001185092.1 , NM_001185092.2
Accession Version	NM_001185092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB111935.1, BC046149.1, AL591806.16 and AI277603.1. On May 31, 2019 this sequence version replaced NM_001185092.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.167558.1, SRR1803612.89339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGCCC 
GTCTCCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172021.1
CDS	73..804
Translation	MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Target ORF information:

RefSeq Version	NM_001185092.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185092.2

ORF Insert Sequence:

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGCCC 
GTCTCCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( NM_001185094.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172023.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01670
Clone ID Related Accession (Same CDS sequence)	NM_001185094.1 , XM_005245216.4 , XM_005245216.3
Accession Version	NM_001185094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI752623.1, BX416745.2, AL591806.16, AF069987.1 and BQ002524.1. Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BX416745.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172023.1
CDS	215..1090
Translation	MAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	NM_001185094.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185094.1

ORF Insert Sequence:

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( NM_005600.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005591.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05088
Clone ID Related Accession (Same CDS sequence)	NM_005600.2 , NM_005600.3
Accession Version	NM_005600.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB111935.1, AF069987.1 and BQ002524.1. On Jun 8, 2010 this sequence version replaced NM_005600.1. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.142313.1, SRR1803614.401178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCAGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCTGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCGACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005591.1
CDS	106..1089
Translation	MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	NM_005600.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005600.2

ORF Insert Sequence:

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCAGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCTGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCGACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( NM_005600.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005591.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.

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CloneID	OHu05088
Clone ID Related Accession (Same CDS sequence)	NM_005600.2 , NM_005600.3
Accession Version	NM_005600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB111935.1, AF069987.1 and BQ002524.1. On Nov 22, 2018 this sequence version replaced NM_005600.2. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.542078.1, SRR1660807.142313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368009.7/ ENSP00000356988.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCAGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCTGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCGACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005591.1
CDS	73..1056
Translation	MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	NM_005600.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005600.3

ORF Insert Sequence:

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCAGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCTGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCGACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( NM_001185093.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172022.1 Homo sapiens nitrilase 1 (NIT1), transcript variant 3, mRNA.

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CloneID	OHu07629
Clone ID Related Accession (Same CDS sequence)	NM_001185093.1
Accession Version	NM_001185093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC302367.1, AK296606.1, AL591806.16, AF069987.1 and BQ002524.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks an exon in the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK296606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCCCAGA 
GCCATGGCTA TCTCCTCTTC CTCCTGCGAA CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
TCGACGCCAG ACAAGCAACA GAACTTTAAA ACATGTGCTG AGCTGGTTCG AGAGGCTGCC 
AGACTGGGTG CCTGCCTGGC TTTCCTGCCT GAGGCATTTG ACTTCATTGC ACGGGACCCT 
GCAGAGACGC TACACCTGTC TGAACCACTG GGTGGGAAAC TTTTGGAAGA ATACACCCAG 
CTTGCCAGGG AATGTGGACT CTGGCTGTCC TTGGGTGGTT TCCATGAGCG TGGCCAAGAC 
TGGGAGCAGA CTCAGAAAAT CTACAATTGT CACGTGCTGC TGAACAGCAA AGGGGCAGTA 
GTGGCCACTT ACAGGAAGAC ACATCTGTGT GACGTAGAGA TTCCAGGGCA GGGGCCTATG 
TGTGAAAGCA ACTCTACCAT GCCTGGGCCC AGTCTTGAGT CACCTGTCAG CACACCAGCA 
GGCAAGATTG GTCTAGCTGT CTGCTATGAC ATGCGGTTCC CTGAACTCTC TCTGGCATTG 
GCTCAAGCTG GAGCAGAGAT ACTTACCTAT CCTTCAGCTT TTGGATCCAT TACAGGCCCA 
GCCCACTGGG AGGTGTTGCT GCGGGCCCGT GCTATCGAAA CCCAGTGCTA TGTAGTGGCA 
GCAGCACAGT GTGGACGCCA CCATGAGAAG AGAGCAAGTT ATGGCCACAG CATGGTGGTA 
GACCCCTGGG GAACAGTGGT GGCCCGCTGC TCTGAGGGGC CAGGCCTCTG CCTTGCCCGA 
ATAGACCTCA ACTATCTGCG ACAGTTGCGC CGACACCTGC CTGTGTTCCA GCACCGCAGG 
CCTGACCTCT ATGGCAATCT GGGTCACCCA CTGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172022.1
CDS	383..1321
Translation	MTFGLRKRLSEERGFSLMPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	NM_001185093.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185093.1

ORF Insert Sequence:

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCCCAGA 
GCCATGGCTA TCTCCTCTTC CTCCTGCGAA CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
TCGACGCCAG ACAAGCAACA GAACTTTAAA ACATGTGCTG AGCTGGTTCG AGAGGCTGCC 
AGACTGGGTG CCTGCCTGGC TTTCCTGCCT GAGGCATTTG ACTTCATTGC ACGGGACCCT 
GCAGAGACGC TACACCTGTC TGAACCACTG GGTGGGAAAC TTTTGGAAGA ATACACCCAG 
CTTGCCAGGG AATGTGGACT CTGGCTGTCC TTGGGTGGTT TCCATGAGCG TGGCCAAGAC 
TGGGAGCAGA CTCAGAAAAT CTACAATTGT CACGTGCTGC TGAACAGCAA AGGGGCAGTA 
GTGGCCACTT ACAGGAAGAC ACATCTGTGT GACGTAGAGA TTCCAGGGCA GGGGCCTATG 
TGTGAAAGCA ACTCTACCAT GCCTGGGCCC AGTCTTGAGT CACCTGTCAG CACACCAGCA 
GGCAAGATTG GTCTAGCTGT CTGCTATGAC ATGCGGTTCC CTGAACTCTC TCTGGCATTG 
GCTCAAGCTG GAGCAGAGAT ACTTACCTAT CCTTCAGCTT TTGGATCCAT TACAGGCCCA 
GCCCACTGGG AGGTGTTGCT GCGGGCCCGT GCTATCGAAA CCCAGTGCTA TGTAGTGGCA 
GCAGCACAGT GTGGACGCCA CCATGAGAAG AGAGCAAGTT ATGGCCACAG CATGGTGGTA 
GACCCCTGGG GAACAGTGGT GGCCCGCTGC TCTGAGGGGC CAGGCCTCTG CCTTGCCCGA 
ATAGACCTCA ACTATCTGCG ACAGTTGCGC CGACACCTGC CTGTGTTCCA GCACCGCAGG 
CCTGACCTCT ATGGCAATCT GGGTCACCCA CTGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_005245216.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245273.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu01670
Clone ID Related Accession (Same CDS sequence)	NM_001185094.1 , XM_005245216.4 , XM_005245216.3
Accession Version	XM_005245216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245216.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245273.1
CDS	243..1118
Translation	MAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_005245216.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245216.4

ORF Insert Sequence:

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_005245216.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245273.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu01670
Clone ID Related Accession (Same CDS sequence)	NM_001185094.1 , XM_005245216.4 , XM_005245216.3
Accession Version	XM_005245216.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitrilase homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005245216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245273.1
CDS	237..1112
Translation	MAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_005245216.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245216.3

ORF Insert Sequence:

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGG TGTTGCTGCG GGCCCGTGCT ATCGAAACCC AGTGCTATGT AGTGGCAGCA 
GCACAGTGTG GACGCCACCA TGAGAAGAGA GCAAGTTATG GCCACAGCAT GGTGGTAGAC 
CCCTGGGGAA CAGTGGTGGC CCGCTGCTCT GAGGGGCCAG GCCTCTGCCT TGCCCGAATA 
GACCTCAACT ATCTGCGACA GTTGCGCCGA CACCTGCCTG TGTTCCAGCA CCGCAGGCCT 
GACCTCTATG GCAATCTGGG TCACCCACTG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_005245215.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245272.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34413
Clone ID Related Accession (Same CDS sequence)	XM_005245215.4 , XM_005245215.5
Accession Version	XM_005245215.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitrilase homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245215.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245272.1
CDS	67..1101
Translation	MVLAISSCRTYSLSRRPRLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_005245215.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245215.4

ORF Insert Sequence:

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_005245215.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245272.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu34413
Clone ID Related Accession (Same CDS sequence)	XM_005245215.4 , XM_005245215.5
Accession Version	XM_005245215.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245215.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245272.1
CDS	66..1100
Translation	MVLAISSCRTYSLSRRPRLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_005245215.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245215.5

ORF Insert Sequence:

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_005245214.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245271.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34414
Clone ID Related Accession (Same CDS sequence)	XM_005245214.2
Accession Version	XM_005245214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005245214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245271.1
CDS	406..1440
Translation	MTFGLRKRLSEERGFSLMLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_005245214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245214.2

ORF Insert Sequence:

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGGT GTTGCTGCGG 
GCCCGTGCTA TCGAAACCCA GTGCTATGTA GTGGCAGCAG CACAGTGTGG ACGCCACCAT 
GAGAAGAGAG CAAGTTATGG CCACAGCATG GTGGTAGACC CCTGGGGAAC AGTGGTGGCC 
CGCTGCTCTG AGGGGCCAGG CCTCTGCCTT GCCCGAATAG ACCTCAACTA TCTGCGACAG 
TTGCGCCGAC ACCTGCCTGT GTTCCAGCAC CGCAGGCCTG ACCTCTATGG CAATCTGGGT 
CACCCACTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_006711341.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711404.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu34415
Clone ID Related Accession (Same CDS sequence)	XM_006711341.3
Accession Version	XM_006711341.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitrilase homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGCC CGTCTCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711404.1
CDS	66..848
Translation	MVLAISSCRTYSLSRRPRLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Target ORF information:

RefSeq Version	XM_006711341.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711341.3

ORF Insert Sequence:

ATGGTTTTGG CTATATCTTC ATGTAGGACC TACTCCCTAT CCCGTCGGCC GCGGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGCC CGTCTCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_024447363.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303131.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu105053
Clone ID Related Accession (Same CDS sequence)	XM_024447363.1
Accession Version	XM_024447363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGCC CGTCTCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303131.1
CDS	406..1188
Translation	MTFGLRKRLSEERGFSLMLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Target ORF information:

RefSeq Version	XM_024447363.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447363.1

ORF Insert Sequence:

ATGACTTTTG GACTGCGGAA ACGTTTGTCA GAGGAAAGAG GCTTCAGTTT AATGCTGGGC 
TTCATCACCA GGCCTCCTCA CAGATTCCTG TCCCTTCTGT GTCCTGGACT CCGGATACCT 
CAACTCTCAG TACTTTGTGC TCAGCCCAGG CCCAGAGCCA TGGCTATCTC CTCTTCCTCC 
TGCGAACTGC CCCTGGTGGC TGTGTGCCAG GTAACATCGA CGCCAGACAA GCAACAGAAC 
TTTAAAACAT GTGCTGAGCT GGTTCGAGAG GCTGCCAGAC TGGGTGCCTG CCTGGCTTTC 
CTGCCTGAGG CATTTGACTT CATTGCACGG GACCCTGCAG AGACGCTACA CCTGTCTGAA 
CCACTGGGTG GGAAACTTTT GGAAGAATAC ACCCAGCTTG CCAGGGAATG TGGACTCTGG 
CTGTCCTTGG GTGGTTTCCA TGAGCGTGGC CAAGACTGGG AGCAGACTCA GAAAATCTAC 
AATTGTCACG TGCTGCTGAA CAGCAAAGGG GCAGTAGTGG CCACTTACAG GAAGACACAT 
CTGTGTGACG TAGAGATTCC AGGGCAGGGG CCTATGTGTG AAAGCAACTC TACCATGCCT 
GGGCCCAGTC TTGAGTCACC TGTCAGCACA CCAGCAGGCA AGATTGGTCT AGCTGTCTGC 
TATGACATGC GGTTCCCTGA ACTCTCTCTG GCATTGGCTC AAGCTGGAGC AGAGATACTT 
ACCTATCCTT CAGCTTTTGG ATCCATTACA GGCCCAGCCC ACTGGGAGCC CGTCTCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT1 ( XM_017001371.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856860.1 Homo sapiens nitrilase 1 (NIT1), transcript variant X5, mRNA.

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CloneID	OHu90063
Clone ID Related Accession (Same CDS sequence)	XM_017001371.1
Accession Version	XM_017001371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deaminated glutathione amidase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGC CCGTCTCCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856860.1
CDS	241..864
Translation	MAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Target ORF information:

RefSeq Version	XM_017001371.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitrilase 1 (NIT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001371.1

ORF Insert Sequence:

ATGGCTATCT CCTCTTCCTC CTGCGAACTG CCCCTGGTGG CTGTGTGCCA GGTAACATCG 
ACGCCAGACA AGCAACAGAA CTTTAAAACA TGTGCTGAGC TGGTTCGAGA GGCTGCCAGA 
CTGGGTGCCT GCCTGGCTTT CCTGCCTGAG GCATTTGACT TCATTGCACG GGACCCTGCA 
GAGACGCTAC ACCTGTCTGA ACCACTGGGT GGGAAACTTT TGGAAGAATA CACCCAGCTT 
GCCAGGGAAT GTGGACTCTG GCTGTCCTTG GGTGGTTTCC ATGAGCGTGG CCAAGACTGG 
GAGCAGACTC AGAAAATCTA CAATTGTCAC GTGCTGCTGA ACAGCAAAGG GGCAGTAGTG 
GCCACTTACA GGAAGACACA TCTGTGTGAC GTAGAGATTC CAGGGCAGGG GCCTATGTGT 
GAAAGCAACT CTACCATGCC TGGGCCCAGT CTTGAGTCAC CTGTCAGCAC ACCAGCAGGC 
AAGATTGGTC TAGCTGTCTG CTATGACATG CGGTTCCCTG AACTCTCTCT GGCATTGGCT 
CAAGCTGGAG CAGAGATACT TACCTATCCT TCAGCTTTTG GATCCATTAC AGGCCCAGCC 
CACTGGGAGC CCGTCTCCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NIT1
Entrez Gene ID	4817
Full Name	nitrilase 1
General protein information	Preferred Names nitrilase 1 Names deaminated glutathione amidase nitrilase homolog 1 dGSH amidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.3
Summary	This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].
Disorder MIM:	604618

Danio rerio (zebrafish)	nit1	NP_001004638.1
Mus musculus (house mouse)	Nit1	NP_036179.1
Rattus norvegicus (Norway rat)	Nit1	NP_872609.2
Gallus gallus (chicken)	NIT1	XP_003642751.1
Saccharomyces cerevisiae (baker's yeast)	NIT2	NP_012409.1
Homo sapiens (human)	NIT1	NP_005591.1
	NIT1	XP_001115539.1
Schizosaccharomyces pombe (fission yeast)	SPBC651.02	NP_595500.1
Neurospora crassa	NCU05757	XP_959732.2
Oryza sativa (rice)	Os12g0502500	NP_001066830.1
Pan troglodytes (chimpanzee)	NIT1	XP_001152578.1
Canis lupus familiaris (dog)	NIT1	XP_536134.3
Arabidopsis thaliana (thale cress)	AT4G08790	NP_567340.1
Bos taurus (cattle)	NIT1	NP_001033112.1
Kluyveromyces lactis	KLLA0E15247g	XP_454637.1
Xenopus tropicalis (tropical clawed frog)	nit1	XP_004919403.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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30% OFF

NIT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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