TONSL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TONSL
Entrez Gene ID	4796
Full Name	tonsoku like, DNA repair protein
Synonyms	IKBR,NFKBIL2
General protein information	Preferred Names tonsoku like, DNA repair protein Names tonsoku-like protein I-kappa-B-related protein NF-kappa-B inhibitor-like protein 2 ikappaBR inhibitor of kappa B-related protein nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.3
Summary	The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008].
Disorder MIM:	604546

mRNA and Protein(s)

mRNA	Protein	Name
NM_013432.4	NP_038460.4	tonsoku-like protein
XM_011517048.2	XP_011515350.1	tonsoku-like protein isoform X1
XM_011517050.2	XP_011515352.1	tonsoku-like protein isoform X3
NM_013432.5	NP_038460.4	tonsoku-like protein
XM_011517049.2	XP_011515351.1	tonsoku-like protein isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:171416	NP_001104618.1
Xenopus tropicalis (tropical clawed frog)	tonsl	XP_002944768.2
Homo sapiens (human)	TONSL	NP_038460.4
	TONSL	XP_002805574.1
Canis lupus familiaris (dog)	TONSL	XP_005628146.1
Bos taurus (cattle)	TONSL	NP_001068656.1
Mus musculus (house mouse)	Tonsl	NP_898914.3
Rattus norvegicus (Norway rat)	Tonsl	NP_001124044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006055	XP_316104.4
Pan troglodytes (chimpanzee)	TONSL	XP_520018.3
Drosophila melanogaster (fruit fly)	CG7457	NP_648150.1

Gene Ontology
Bibliography

Related articles in PubMed

The MMS22L-TONSL heterodimer directly promotes RAD51-dependent recombination upon replication stress.
Piwko W, Mlejnkova LJ, Mutreja K, Ranjha L, Stafa D, Smirnov A, Brodersen MM, Zellweger R, Sturzenegger A, Janscak P, Lopes M, Peter M, Cejka P
The EMBO journal35(23)2584-2601(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

By specifically regulating RAD51 activity at uncoupled replication forks, MMS22L-TONSL stabilizes perturbed replication forks by promoting replication fork reversal and stimulating their homologous recombination-mediated restart in vivo.
Title: The MMS22L-TONSL heterodimer directly promotes RAD51-dependent recombination upon replication stress.

new histones incorporated during DNA replication provide a signature of post-replicative chromatin, read by the human TONSL-MMS22L homologous recombination complex
Title: H4K20me0 marks post-replicative chromatin and recruits the TONSL?MMS22L DNA repair complex.

Results strongly suggest that the Mms22L-Nfkbil2 complex contributes to genome stability by regulating the chromatin state at stalled replication forks.
Title: RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells.

This study identifies MMS22L-NFKBIL2 as components of the replication stress control pathway.
Title: A genome-wide camptothecin sensitivity screen identifies a mammalian MMS22L-NFKBIL2 complex required for genomic stability.

Common NFKBIL2 polymorphisms are associated with susceptibility to invasive pneumococcal infection.
Title: Common NFKBIL2 polymorphisms and susceptibility to pneumococcal disease: a genetic association study.

The following TONSL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TONSL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu59268	XM_011517050.2 Latest version!	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59266	XM_011517048.2 Latest version!	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu59267	XM_011517049.2 Latest version!	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu32001	NM_013432.5 Latest version!	Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu32001	NM_013432.4	Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TONSL ( XM_011517050.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515352.1 Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu59268
Clone ID Related Accession (Same CDS sequence)	XM_011517050.2
Accession Version	XM_011517050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tonsoku-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGTGGG GTCCGGCCCC GCCCCCTCCC ATCCGGGTTC GAGTTCAAGT 
TCAGGATCAT CTCTTCCTCA TCCCTGTCCC ACACAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515352.1
CDS	75..2936
Translation	MSLERELRQLSKAKAKAQRAGQRREEAALCHQLGELLAGHGRYAEALEQHWQELQLRERADDPLGCAVAHRKIGERLAEMEDYPAALQHQHQYLELAHSLRNHTELQRAWATIGRTHLDIYDHCQSRDALLQAQAAFEKSLAIVDEELEGTLAQGELNEMRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARYNLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFLAAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQAHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLVGSGPAPSHPGSSSSSGSSLPHPCPTQQ

Target ORF information:

RefSeq Version	XM_011517050.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517050.2

ORF Insert Sequence:

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGTGGG GTCCGGCCCC GCCCCCTCCC ATCCGGGTTC GAGTTCAAGT 
TCAGGATCAT CTCTTCCTCA TCCCTGTCCC ACACAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TONSL ( XM_011517048.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515350.1 Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59266
Clone ID Related Accession (Same CDS sequence)	XM_011517048.2
Accession Version	XM_011517048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tonsoku-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCA GCCCCTGGCC TCACTGGCAC TGCCCCCAGC TGCGTTTTGC TGAGCTGCTG 
GACAGACCGG GTGCTGAGCG GGCCATCATC CACGTGTCCC TGGCCACCAC ACTGGGAGAC 
ATGAAGGACC ACCATGGGGC CGTGCGCCAC TATGAGGAGG AACTGAGGCT GCGCAGCGGC 
AACGTGCTGG AGGAGGCCAA GACCTGGCTG AACATTGCAC TGTCCCGCGA GGAGGCCGGC 
GATGCCTACG AGCTGCTGGC CCCGTGCTTC CAGAAAGCGC TCAGCTGTGC CCAGCAGGCC 
CAGCGTCCCC AGCTGCAGAG GCAGGTCTTG CAGCATCTCC ATACCGTGCA GCTGAGGCTG 
CAGCCCCAGG AGGCCCCTGA GACCGAAACC AGACTACGGG AGCTCAGTGT AGCTGAAGAT 
GAAGATGAGG AGGAGGAGGC GGAGGAGGCG GCAGCCACAG CGGAGAGCGA AGCCCTGGAG 
GCCGGCGAGG TGGAGCTCTC AGAGGGCGAG GACGACACCG ATGGCCTGAC CCCGCAGCTG 
GAGGAGGACG AGGAGCTTCA GGGCCACCTG GGCCGGCGGA AGGGGAGCAA GTGGAACCGG 
CGAAACGACA TGGGGGAGAC CCTGCTGCAC CGAGCCTGCA TCGAGGGCCA GCTGCGCCGC 
GTCCAGGACC TTGTGAGGCA GGGCCACCCC CTTAACCCTC GGGACTACTG TGGCTGGACA 
CCTCTGCACG AGGCCTGCAA CTACGGGCAT CTAGAAATTG TCCGCTTCCT GCTGGACCAC 
GGGGCCGCAG TGGACGACCC AGGTGGCCAG GGCTGCGAAG GCATCACCCC CCTCCACGAT 
GCCCTCAACT GTGGCCACTT CGAGGTGGCT GAGCTGCTGC TTGAACGGGG GGCGTCCGTC 
ACCCTCCGCA CTCGAAAGGG CCTCAGCCCG CTGGAGACGC TGCAGCAGTG GGTGAAGCTG 
TACCGCAGGG ACCTGGACCT GGAGACGCGG CAGAAGGCCA GGGCCATGGA GATGCTGCTC 
CAGGCGGCTG CCTCGGGCCA AGATCCCCAC AGCTCCCAGG CCTTCCACAC CCCAAGCAGC 
CTTCTGTTTG ACCCCGAGAC CTCTCCTCCT TTGAGCCCCT GCCCAGAACC CCCCTCTAAT 
AGCACTAGAC TCCCAGAGGC CTCTCAGGCC CATGTCAGGG TCTCCCCAGG GCAGGCGGCA 
CCAGCCATGG CCAGGCCTCG GAGGAGCAGG CATGGGCCAG CCAGCAGCAG CAGCAGCTCA 
GAAGGCGAGG ACAGCGCAGG CCCCGCACGG CCGTCCCAGA AGAGGCCTCG GTGCTCGGCC 
ACAGCACAAC GGGTGGCAGC CTGGACGCCT GGCCCCGCCA GCAACAGGGA AGCAGCCACA 
GCCAGCACCA GCCGGGCAGC CTACCAGGCA GCCATCCGGG GTGTGGGCAG TGCTCAGAGC 
CGGCTGGGGC CTGGCCCACC GCGGGGCCAC AGCAAAGCCC TTGCCCCCCA GGCAGCGCTC 
ATCCCGGAGG AGGAGTGCCT GGCCGGGGAC TGGCTGGAGC TGGACATGCC CCTGACCCGC 
AGCCGCCGGC CCCGCCCCCG GGGCACTGGA GACAACCGCA GGCCCAGTAG TACCTCTGGG 
TCGGACAGTG AGGAGAGCAG GCCCCGTGCC CGAGCCAAGC AGGTCCGCCT GACCTGCATG 
CAGAGTTGCA GTGCGCCAGT TAACGCAGGG CCCAGCAGCC TGGCTTCAGA ACCTCCAGGG 
AGCCCCAGCA CCCCCAGGGT CTCAGAGCCC AGTGGGGACA GCTCTGCGGC AGGCCAGCCC 
TTGGGTCCGG CCCCGCCCCC TCCCATCCGG GTTCGAGTTC AAGTTCAGGA TCATCTCTTC 
CTCATCCCTG TCCCACACAG CAGTGACACC CACTCTGTGG CCTGGCTGGC CGAGCAGGCG 
GCCCAGCGCT ACTACCAGAC CTGCGGGCTG CTGCCCAGGC TCACCCTACG GAAAGAGGGG 
GCCCTGCTGG CCCCACAGGA CCTCATCCCT GATGTGCTGC AGAGCAATGA CGAGGTGTTG 
GCTGAGGTGA CTTCGTGGGA CCTGCCCCCG TTGACTGACC GCTACCGCAG GGCCTGCCAG 
AGCCTGGGGC AAGGGGAGCA CCAACAGGTG CTGCAGGCCG TGGAGCTCCA GGGCTTGGGC 
CTCTCGTTCA GCGCCTGCTC CCTGGCCCTG GACCAGGCCC AGCTTACACC CCTGCTGCGG 
GCCCTCAAGC TGCACACAGC ACTCCGGGAG CTGCGCCTGG CAGGGAACCG GCTGGGGGAC 
AAGTGTGTGG CTGAGCTGGT GGCTGCCCTG GGCACCATGC CCAGCCTGGC CCTCCTTGAC 
CTCTCCTCCA ATCACCTGGG TCCCGAAGGC CTGCGCCAGC TTGCCATGGG GCTCCCAGGC 
CAAGCCACCT TGCAGAGTTT GGAGGAGCTG GACTTAAGCA TGAACCCCCT GGGGGACGGC 
TGTGGCCAGT CCCTGGCCTC CCTCCTGCAC GCCTGCCCCT TACTCAGCAC CCTGCGCCTG 
CAGGCGTGTG GCTTCGGCCC CAGCTTCTTT CTGAGCCACC AGACAGCACT GGGTAGTGCT 
TTCCAAGATG CTGAGCACCT GAAGACCCTG TCCCTGTCCT ACAACGCCCT GGGAGCCCCT 
GCCCTGGCCA GGACCCTGCA GAGCCTGCCC GCCGGCACCC TCCTGCACTT AGAGCTCAGC 
TCCGTGGCAG CCGGCAAGGG TGATTCGGAC CTCATGGAGC CTGTATTCCG ATACCTGGCC 
AAGGAAGGCT GTGCTCTAGC CCACCTGACC CTGTCTGCAA ACCACCTGGG GGACAAGGCT 
GTTAGAGACC TGTGCAGATG TCTCTCTCTG TGCCCCTCAC TCATCTCACT GGATCTGTCT 
GCCAACCCTG AGATCAGCTG TGCCAGCTTG GAAGAGCTCC TGTCCACCCT CCAAAAGCGG 
CCCCAAGGCC TTAGCTTCCT TGGCCTGTCA GGCTGCGCCG TCCAGGGTCC CCTGGGCCTG 
GGCCTGTGGG ACAAGATAGC CGCGCAGCTC CGGGAACTGC AGCTGTGCAG CAGACGCCTC 
TGCGCTGAGG ACAGGGACGC CCTGCGCCAG CTGCAGCCCA GTCGGCCGGG CCCCGGCGAG 
TGCACGCTGG ACCACGGCTC CAAGCTCTTC TTTCGGCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515350.1
CDS	87..3251
Translation	MGGSPWPHWHCPQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQAHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPGPGECTLDHGSKLFFRRL

Target ORF information:

RefSeq Version	XM_011517048.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517048.2

ORF Insert Sequence:

ATGGGTGGCA GCCCCTGGCC TCACTGGCAC TGCCCCCAGC TGCGTTTTGC TGAGCTGCTG 
GACAGACCGG GTGCTGAGCG GGCCATCATC CACGTGTCCC TGGCCACCAC ACTGGGAGAC 
ATGAAGGACC ACCATGGGGC CGTGCGCCAC TATGAGGAGG AACTGAGGCT GCGCAGCGGC 
AACGTGCTGG AGGAGGCCAA GACCTGGCTG AACATTGCAC TGTCCCGCGA GGAGGCCGGC 
GATGCCTACG AGCTGCTGGC CCCGTGCTTC CAGAAAGCGC TCAGCTGTGC CCAGCAGGCC 
CAGCGTCCCC AGCTGCAGAG GCAGGTCTTG CAGCATCTCC ATACCGTGCA GCTGAGGCTG 
CAGCCCCAGG AGGCCCCTGA GACCGAAACC AGACTACGGG AGCTCAGTGT AGCTGAAGAT 
GAAGATGAGG AGGAGGAGGC GGAGGAGGCG GCAGCCACAG CGGAGAGCGA AGCCCTGGAG 
GCCGGCGAGG TGGAGCTCTC AGAGGGCGAG GACGACACCG ATGGCCTGAC CCCGCAGCTG 
GAGGAGGACG AGGAGCTTCA GGGCCACCTG GGCCGGCGGA AGGGGAGCAA GTGGAACCGG 
CGAAACGACA TGGGGGAGAC CCTGCTGCAC CGAGCCTGCA TCGAGGGCCA GCTGCGCCGC 
GTCCAGGACC TTGTGAGGCA GGGCCACCCC CTTAACCCTC GGGACTACTG TGGCTGGACA 
CCTCTGCACG AGGCCTGCAA CTACGGGCAT CTAGAAATTG TCCGCTTCCT GCTGGACCAC 
GGGGCCGCAG TGGACGACCC AGGTGGCCAG GGCTGCGAAG GCATCACCCC CCTCCACGAT 
GCCCTCAACT GTGGCCACTT CGAGGTGGCT GAGCTGCTGC TTGAACGGGG GGCGTCCGTC 
ACCCTCCGCA CTCGAAAGGG CCTCAGCCCG CTGGAGACGC TGCAGCAGTG GGTGAAGCTG 
TACCGCAGGG ACCTGGACCT GGAGACGCGG CAGAAGGCCA GGGCCATGGA GATGCTGCTC 
CAGGCGGCTG CCTCGGGCCA AGATCCCCAC AGCTCCCAGG CCTTCCACAC CCCAAGCAGC 
CTTCTGTTTG ACCCCGAGAC CTCTCCTCCT TTGAGCCCCT GCCCAGAACC CCCCTCTAAT 
AGCACTAGAC TCCCAGAGGC CTCTCAGGCC CATGTCAGGG TCTCCCCAGG GCAGGCGGCA 
CCAGCCATGG CCAGGCCTCG GAGGAGCAGG CATGGGCCAG CCAGCAGCAG CAGCAGCTCA 
GAAGGCGAGG ACAGCGCAGG CCCCGCACGG CCGTCCCAGA AGAGGCCTCG GTGCTCGGCC 
ACAGCACAAC GGGTGGCAGC CTGGACGCCT GGCCCCGCCA GCAACAGGGA AGCAGCCACA 
GCCAGCACCA GCCGGGCAGC CTACCAGGCA GCCATCCGGG GTGTGGGCAG TGCTCAGAGC 
CGGCTGGGGC CTGGCCCACC GCGGGGCCAC AGCAAAGCCC TTGCCCCCCA GGCAGCGCTC 
ATCCCGGAGG AGGAGTGCCT GGCCGGGGAC TGGCTGGAGC TGGACATGCC CCTGACCCGC 
AGCCGCCGGC CCCGCCCCCG GGGCACTGGA GACAACCGCA GGCCCAGTAG TACCTCTGGG 
TCGGACAGTG AGGAGAGCAG GCCCCGTGCC CGAGCCAAGC AGGTCCGCCT GACCTGCATG 
CAGAGTTGCA GTGCGCCAGT TAACGCAGGG CCCAGCAGCC TGGCTTCAGA ACCTCCAGGG 
AGCCCCAGCA CCCCCAGGGT CTCAGAGCCC AGTGGGGACA GCTCTGCGGC AGGCCAGCCC 
TTGGGTCCGG CCCCGCCCCC TCCCATCCGG GTTCGAGTTC AAGTTCAGGA TCATCTCTTC 
CTCATCCCTG TCCCACACAG CAGTGACACC CACTCTGTGG CCTGGCTGGC CGAGCAGGCG 
GCCCAGCGCT ACTACCAGAC CTGCGGGCTG CTGCCCAGGC TCACCCTACG GAAAGAGGGG 
GCCCTGCTGG CCCCACAGGA CCTCATCCCT GATGTGCTGC AGAGCAATGA CGAGGTGTTG 
GCTGAGGTGA CTTCGTGGGA CCTGCCCCCG TTGACTGACC GCTACCGCAG GGCCTGCCAG 
AGCCTGGGGC AAGGGGAGCA CCAACAGGTG CTGCAGGCCG TGGAGCTCCA GGGCTTGGGC 
CTCTCGTTCA GCGCCTGCTC CCTGGCCCTG GACCAGGCCC AGCTTACACC CCTGCTGCGG 
GCCCTCAAGC TGCACACAGC ACTCCGGGAG CTGCGCCTGG CAGGGAACCG GCTGGGGGAC 
AAGTGTGTGG CTGAGCTGGT GGCTGCCCTG GGCACCATGC CCAGCCTGGC CCTCCTTGAC 
CTCTCCTCCA ATCACCTGGG TCCCGAAGGC CTGCGCCAGC TTGCCATGGG GCTCCCAGGC 
CAAGCCACCT TGCAGAGTTT GGAGGAGCTG GACTTAAGCA TGAACCCCCT GGGGGACGGC 
TGTGGCCAGT CCCTGGCCTC CCTCCTGCAC GCCTGCCCCT TACTCAGCAC CCTGCGCCTG 
CAGGCGTGTG GCTTCGGCCC CAGCTTCTTT CTGAGCCACC AGACAGCACT GGGTAGTGCT 
TTCCAAGATG CTGAGCACCT GAAGACCCTG TCCCTGTCCT ACAACGCCCT GGGAGCCCCT 
GCCCTGGCCA GGACCCTGCA GAGCCTGCCC GCCGGCACCC TCCTGCACTT AGAGCTCAGC 
TCCGTGGCAG CCGGCAAGGG TGATTCGGAC CTCATGGAGC CTGTATTCCG ATACCTGGCC 
AAGGAAGGCT GTGCTCTAGC CCACCTGACC CTGTCTGCAA ACCACCTGGG GGACAAGGCT 
GTTAGAGACC TGTGCAGATG TCTCTCTCTG TGCCCCTCAC TCATCTCACT GGATCTGTCT 
GCCAACCCTG AGATCAGCTG TGCCAGCTTG GAAGAGCTCC TGTCCACCCT CCAAAAGCGG 
CCCCAAGGCC TTAGCTTCCT TGGCCTGTCA GGCTGCGCCG TCCAGGGTCC CCTGGGCCTG 
GGCCTGTGGG ACAAGATAGC CGCGCAGCTC CGGGAACTGC AGCTGTGCAG CAGACGCCTC 
TGCGCTGAGG ACAGGGACGC CCTGCGCCAG CTGCAGCCCA GTCGGCCGGG CCCCGGCGAG 
TGCACGCTGG ACCACGGCTC CAAGCTCTTC TTTCGGCGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TONSL ( XM_011517049.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515351.1 Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X2, mRNA.

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CloneID	OHu59267
Clone ID Related Accession (Same CDS sequence)	XM_011517049.2
Accession Version	XM_011517049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tonsoku-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT TTGCTGAGCT GCTGGACAGA CCGGGTGCTG AGCGGGCCAT CATCCACGTG 
TCCCTGGCCA CCACACTGGG AGACATGAAG GACCACCATG GGGCCGTGCG CCACTATGAG 
GAGGAACTGA GGCTGCGCAG CGGCAACGTG CTGGAGGAGG CCAAGACCTG GCTGAACATT 
GCACTGTCCC GCGAGGAGGC CGGCGATGCC TACGAGCTGC TGGCCCCGTG CTTCCAGAAA 
GCGCTCAGCT GTGCCCAGCA GGCCCAGCGT CCCCAGCTGC AGAGGCAGGT CTTGCAGCAT 
CTCCATACCG TGCAGCTGAG GCTGCAGCCC CAGGAGGCCC CTGAGACCGA AACCAGACTA 
CGGGAGCTCA GTGTAGCTGA AGATGAAGAT GAGGAGGAGG AGGCGGAGGA GGCGGCAGCC 
ACAGCGGAGA GCGAAGCCCT GGAGGCCGGC GAGGTGGAGC TCTCAGAGGG CGAGGACGAC 
ACCGATGGCC TGACCCCGCA GCTGGAGGAG GACGAGGAGC TTCAGGGCCA CCTGGGCCGG 
CGGAAGGGGA GCAAGTGGAA CCGGCGAAAC GACATGGGGG AGACCCTGCT GCACCGAGCC 
TGCATCGAGG GCCAGCTGCG CCGCGTCCAG GACCTTGTGA GGCAGGGCCA CCCCCTTAAC 
CCTCGGGACT ACTGTGGCTG GACACCTCTG CACGAGGCCT GCAACTACGG GCATCTAGAA 
ATTGTCCGCT TCCTGCTGGA CCACGGGGCC GCAGTGGACG ACCCAGGTGG CCAGGGCTGC 
GAAGGCATCA CCCCCCTCCA CGATGCCCTC AACTGTGGCC ACTTCGAGGT GGCTGAGCTG 
CTGCTTGAAC GGGGGGCGTC CGTCACCCTC CGCACTCGAA AGGGCCTCAG CCCGCTGGAG 
ACGCTGCAGC AGTGGGTGAA GCTGTACCGC AGGGACCTGG ACCTGGAGAC GCGGCAGAAG 
GCCAGGGCCA TGGAGATGCT GCTCCAGGCG GCTGCCTCGG GCCAAGATCC CCACAGCTCC 
CAGGCCTTCC ACACCCCAAG CAGCCTTCTG TTTGACCCCG AGACCTCTCC TCCTTTGAGC 
CCCTGCCCAG AACCCCCCTC TAATAGCACT AGACTCCCAG AGGCCTCTCA GGCCCATGTC 
AGGGTCTCCC CAGGGCAGGC GGCACCAGCC ATGGCCAGGC CTCGGAGGAG CAGGCATGGG 
CCAGCCAGCA GCAGCAGCAG CTCAGAAGGC GAGGACAGCG CAGGCCCCGC ACGGCCGTCC 
CAGAAGAGGC CTCGGTGCTC GGCCACAGCA CAACGGGTGG CAGCCTGGAC GCCTGGCCCC 
GCCAGCAACA GGGAAGCAGC CACAGCCAGC ACCAGCCGGG CAGCCTACCA GGCAGCCATC 
CGGGGTGTGG GCAGTGCTCA GAGCCGGCTG GGGCCTGGCC CACCGCGGGG CCACAGCAAA 
GCCCTTGCCC CCCAGGCAGC GCTCATCCCG GAGGAGGAGT GCCTGGCCGG GGACTGGCTG 
GAGCTGGACA TGCCCCTGAC CCGCAGCCGC CGGCCCCGCC CCCGGGGCAC TGGAGACAAC 
CGCAGGCCCA GTAGTACCTC TGGGTCGGAC AGTGAGGAGA GCAGGCCCCG TGCCCGAGCC 
AAGCAGGTCC GCCTGACCTG CATGCAGAGT TGCAGTGCGC CAGTTAACGC AGGGCCCAGC 
AGCCTGGCTT CAGAACCTCC AGGGAGCCCC AGCACCCCCA GGGTCTCAGA GCCCAGTGGG 
GACAGCTCTG CGGCAGGCCA GCCCTTGGGT CCGGCCCCGC CCCCTCCCAT CCGGGTTCGA 
GTTCAAGTTC AGGATCATCT CTTCCTCATC CCTGTCCCAC ACAGCAGTGA CACCCACTCT 
GTGGCCTGGC TGGCCGAGCA GGCGGCCCAG CGCTACTACC AGACCTGCGG GCTGCTGCCC 
AGGCTCACCC TACGGAAAGA GGGGGCCCTG CTGGCCCCAC AGGACCTCAT CCCTGATGTG 
CTGCAGAGCA ATGACGAGGT GTTGGCTGAG GTGACTTCGT GGGACCTGCC CCCGTTGACT 
GACCGCTACC GCAGGGCCTG CCAGAGCCTG GGGCAAGGGG AGCACCAACA GGTGCTGCAG 
GCCGTGGAGC TCCAGGGCTT GGGCCTCTCG TTCAGCGCCT GCTCCCTGGC CCTGGACCAG 
GCCCAGCTTA CACCCCTGCT GCGGGCCCTC AAGCTGCACA CAGCACTCCG GGAGCTGCGC 
CTGGCAGGGA ACCGGCTGGG GGACAAGTGT GTGGCTGAGC TGGTGGCTGC CCTGGGCACC 
ATGCCCAGCC TGGCCCTCCT TGACCTCTCC TCCAATCACC TGGGTCCCGA AGGCCTGCGC 
CAGCTTGCCA TGGGGCTCCC AGGCCAAGCC ACCTTGCAGA GTTTGGAGGA GCTGGACTTA 
AGCATGAACC CCCTGGGGGA CGGCTGTGGC CAGTCCCTGG CCTCCCTCCT GCACGCCTGC 
CCCTTACTCA GCACCCTGCG CCTGCAGGCG TGTGGCTTCG GCCCCAGCTT CTTTCTGAGC 
CACCAGACAG CACTGGGTAG TGCTTTCCAA GATGCTGAGC ACCTGAAGAC CCTGTCCCTG 
TCCTACAACG CCCTGGGAGC CCCTGCCCTG GCCAGGACCC TGCAGAGCCT GCCCGCCGGC 
ACCCTCCTGC ACTTAGAGCT CAGCTCCGTG GCAGCCGGCA AGGGTGATTC GGACCTCATG 
GAGCCTGTAT TCCGATACCT GGCCAAGGAA GGCTGTGCTC TAGCCCACCT GACCCTGTCT 
GCAAACCACC TGGGGGACAA GGCTGTTAGA GACCTGTGCA GATGTCTCTC TCTGTGCCCC 
TCACTCATCT CACTGGATCT GTCTGCCAAC CCTGAGATCA GCTGTGCCAG CTTGGAAGAG 
CTCCTGTCCA CCCTCCAAAA GCGGCCCCAA GGCCTTAGCT TCCTTGGCCT GTCAGGCTGC 
GCCGTCCAGG GTCCCCTGGG CCTGGGCCTG TGGGACAAGA TAGCCGCGCA GCTCCGGGAA 
CTGCAGCTGT GCAGCAGACG CCTCTGCGCT GAGGACAGGG ACGCCCTGCG CCAGCTGCAG 
CCCAGTCGGC CGGGCCCCGG CGAGTGCACG CTGGACCACG GCTCCAAGCT CTTCTTTCGG 
CGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515351.1
CDS	84..3212
Translation	MLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQAHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPGPGECTLDHGSKLFFRRL

Target ORF information:

RefSeq Version	XM_011517049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tonsoku like, DNA repair protein (TONSL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517049.2

ORF Insert Sequence:

ATGCTGCGTT TTGCTGAGCT GCTGGACAGA CCGGGTGCTG AGCGGGCCAT CATCCACGTG 
TCCCTGGCCA CCACACTGGG AGACATGAAG GACCACCATG GGGCCGTGCG CCACTATGAG 
GAGGAACTGA GGCTGCGCAG CGGCAACGTG CTGGAGGAGG CCAAGACCTG GCTGAACATT 
GCACTGTCCC GCGAGGAGGC CGGCGATGCC TACGAGCTGC TGGCCCCGTG CTTCCAGAAA 
GCGCTCAGCT GTGCCCAGCA GGCCCAGCGT CCCCAGCTGC AGAGGCAGGT CTTGCAGCAT 
CTCCATACCG TGCAGCTGAG GCTGCAGCCC CAGGAGGCCC CTGAGACCGA AACCAGACTA 
CGGGAGCTCA GTGTAGCTGA AGATGAAGAT GAGGAGGAGG AGGCGGAGGA GGCGGCAGCC 
ACAGCGGAGA GCGAAGCCCT GGAGGCCGGC GAGGTGGAGC TCTCAGAGGG CGAGGACGAC 
ACCGATGGCC TGACCCCGCA GCTGGAGGAG GACGAGGAGC TTCAGGGCCA CCTGGGCCGG 
CGGAAGGGGA GCAAGTGGAA CCGGCGAAAC GACATGGGGG AGACCCTGCT GCACCGAGCC 
TGCATCGAGG GCCAGCTGCG CCGCGTCCAG GACCTTGTGA GGCAGGGCCA CCCCCTTAAC 
CCTCGGGACT ACTGTGGCTG GACACCTCTG CACGAGGCCT GCAACTACGG GCATCTAGAA 
ATTGTCCGCT TCCTGCTGGA CCACGGGGCC GCAGTGGACG ACCCAGGTGG CCAGGGCTGC 
GAAGGCATCA CCCCCCTCCA CGATGCCCTC AACTGTGGCC ACTTCGAGGT GGCTGAGCTG 
CTGCTTGAAC GGGGGGCGTC CGTCACCCTC CGCACTCGAA AGGGCCTCAG CCCGCTGGAG 
ACGCTGCAGC AGTGGGTGAA GCTGTACCGC AGGGACCTGG ACCTGGAGAC GCGGCAGAAG 
GCCAGGGCCA TGGAGATGCT GCTCCAGGCG GCTGCCTCGG GCCAAGATCC CCACAGCTCC 
CAGGCCTTCC ACACCCCAAG CAGCCTTCTG TTTGACCCCG AGACCTCTCC TCCTTTGAGC 
CCCTGCCCAG AACCCCCCTC TAATAGCACT AGACTCCCAG AGGCCTCTCA GGCCCATGTC 
AGGGTCTCCC CAGGGCAGGC GGCACCAGCC ATGGCCAGGC CTCGGAGGAG CAGGCATGGG 
CCAGCCAGCA GCAGCAGCAG CTCAGAAGGC GAGGACAGCG CAGGCCCCGC ACGGCCGTCC 
CAGAAGAGGC CTCGGTGCTC GGCCACAGCA CAACGGGTGG CAGCCTGGAC GCCTGGCCCC 
GCCAGCAACA GGGAAGCAGC CACAGCCAGC ACCAGCCGGG CAGCCTACCA GGCAGCCATC 
CGGGGTGTGG GCAGTGCTCA GAGCCGGCTG GGGCCTGGCC CACCGCGGGG CCACAGCAAA 
GCCCTTGCCC CCCAGGCAGC GCTCATCCCG GAGGAGGAGT GCCTGGCCGG GGACTGGCTG 
GAGCTGGACA TGCCCCTGAC CCGCAGCCGC CGGCCCCGCC CCCGGGGCAC TGGAGACAAC 
CGCAGGCCCA GTAGTACCTC TGGGTCGGAC AGTGAGGAGA GCAGGCCCCG TGCCCGAGCC 
AAGCAGGTCC GCCTGACCTG CATGCAGAGT TGCAGTGCGC CAGTTAACGC AGGGCCCAGC 
AGCCTGGCTT CAGAACCTCC AGGGAGCCCC AGCACCCCCA GGGTCTCAGA GCCCAGTGGG 
GACAGCTCTG CGGCAGGCCA GCCCTTGGGT CCGGCCCCGC CCCCTCCCAT CCGGGTTCGA 
GTTCAAGTTC AGGATCATCT CTTCCTCATC CCTGTCCCAC ACAGCAGTGA CACCCACTCT 
GTGGCCTGGC TGGCCGAGCA GGCGGCCCAG CGCTACTACC AGACCTGCGG GCTGCTGCCC 
AGGCTCACCC TACGGAAAGA GGGGGCCCTG CTGGCCCCAC AGGACCTCAT CCCTGATGTG 
CTGCAGAGCA ATGACGAGGT GTTGGCTGAG GTGACTTCGT GGGACCTGCC CCCGTTGACT 
GACCGCTACC GCAGGGCCTG CCAGAGCCTG GGGCAAGGGG AGCACCAACA GGTGCTGCAG 
GCCGTGGAGC TCCAGGGCTT GGGCCTCTCG TTCAGCGCCT GCTCCCTGGC CCTGGACCAG 
GCCCAGCTTA CACCCCTGCT GCGGGCCCTC AAGCTGCACA CAGCACTCCG GGAGCTGCGC 
CTGGCAGGGA ACCGGCTGGG GGACAAGTGT GTGGCTGAGC TGGTGGCTGC CCTGGGCACC 
ATGCCCAGCC TGGCCCTCCT TGACCTCTCC TCCAATCACC TGGGTCCCGA AGGCCTGCGC 
CAGCTTGCCA TGGGGCTCCC AGGCCAAGCC ACCTTGCAGA GTTTGGAGGA GCTGGACTTA 
AGCATGAACC CCCTGGGGGA CGGCTGTGGC CAGTCCCTGG CCTCCCTCCT GCACGCCTGC 
CCCTTACTCA GCACCCTGCG CCTGCAGGCG TGTGGCTTCG GCCCCAGCTT CTTTCTGAGC 
CACCAGACAG CACTGGGTAG TGCTTTCCAA GATGCTGAGC ACCTGAAGAC CCTGTCCCTG 
TCCTACAACG CCCTGGGAGC CCCTGCCCTG GCCAGGACCC TGCAGAGCCT GCCCGCCGGC 
ACCCTCCTGC ACTTAGAGCT CAGCTCCGTG GCAGCCGGCA AGGGTGATTC GGACCTCATG 
GAGCCTGTAT TCCGATACCT GGCCAAGGAA GGCTGTGCTC TAGCCCACCT GACCCTGTCT 
GCAAACCACC TGGGGGACAA GGCTGTTAGA GACCTGTGCA GATGTCTCTC TCTGTGCCCC 
TCACTCATCT CACTGGATCT GTCTGCCAAC CCTGAGATCA GCTGTGCCAG CTTGGAAGAG 
CTCCTGTCCA CCCTCCAAAA GCGGCCCCAA GGCCTTAGCT TCCTTGGCCT GTCAGGCTGC 
GCCGTCCAGG GTCCCCTGGG CCTGGGCCTG TGGGACAAGA TAGCCGCGCA GCTCCGGGAA 
CTGCAGCTGT GCAGCAGACG CCTCTGCGCT GAGGACAGGG ACGCCCTGCG CCAGCTGCAG 
CCCAGTCGGC CGGGCCCCGG CGAGTGCACG CTGGACCACG GCTCCAAGCT CTTCTTTCGG 
CGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TONSL ( NM_013432.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_038460.4 Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.

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CloneID	OHu32001
Clone ID Related Accession (Same CDS sequence)	NM_013432.4 , NM_013432.5
Accession Version	NM_013432.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	tonsoku-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN366457.1, BC008782.2, AC084125.9 and BF475381.1. On May 7, 2008 this sequence version replaced NM_013432.3. ##Evidence-Data-START## Transcript exon combination :: BC008782.2, SRR1803616.59587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGGTCC GGCCCCGCCC CCTCCCATCC GGGTTCGAGT TCAAGTTCAG 
GATCATCTCT TCCTCATCCC TGTCCCACAC AGCAGTGACA CCCACTCTGT GGCCTGGCTG 
GCCGAGCAGG CGGCCCAGCG CTACTACCAG ACCTGCGGGC TGCTGCCCAG GCTCACCCTA 
CGGAAAGAGG GGGCCCTGCT GGCCCCACAG GACCTCATCC CTGATGTGCT GCAGAGCAAT 
GACGAGGTGT TGGCTGAGGT GACTTCGTGG GACCTGCCCC CGTTGACTGA CCGCTACCGC 
AGGGCCTGCC AGAGCCTGGG GCAAGGGGAG CACCAACAGG TGCTGCAGGC CGTGGAGCTC 
CAGGGCTTGG GCCTCTCGTT CAGCGCCTGC TCCCTGGCCC TGGACCAGGC CCAGCTTACA 
CCCCTGCTGC GGGCCCTCAA GCTGCACACA GCACTCCGGG AGCTGCGCCT GGCAGGGAAC 
CGGCTGGGGG ACAAGTGTGT GGCTGAGCTG GTGGCTGCCC TGGGCACCAT GCCCAGCCTG 
GCCCTCCTTG ACCTCTCCTC CAATCACCTG GGTCCCGAAG GCCTGCGCCA GCTTGCCATG 
GGGCTCCCAG GCCAAGCCAC CTTGCAGAGT TTGGAGGAGC TGGACTTAAG CATGAACCCC 
CTGGGGGACG GCTGTGGCCA GTCCCTGGCC TCCCTCCTGC ACGCCTGCCC CTTACTCAGC 
ACCCTGCGCC TGCAGGCGTG TGGCTTCGGC CCCAGCTTCT TTCTGAGCCA CCAGACAGCA 
CTGGGTAGTG CTTTCCAAGA TGCTGAGCAC CTGAAGACCC TGTCCCTGTC CTACAACGCC 
CTGGGAGCCC CTGCCCTGGC CAGGACCCTG CAGAGCCTGC CCGCCGGCAC CCTCCTGCAC 
TTAGAGCTCA GCTCCGTGGC AGCCGGCAAG GGTGATTCGG ACCTCATGGA GCCTGTATTC 
CGATACCTGG CCAAGGAAGG CTGTGCTCTA GCCCACCTGA CCCTGTCTGC AAACCACCTG 
GGGGACAAGG CTGTTAGAGA CCTGTGCAGA TGTCTCTCTC TGTGCCCCTC ACTCATCTCA 
CTGGATCTGT CTGCCAACCC TGAGATCAGC TGTGCCAGCT TGGAAGAGCT CCTGTCCACC 
CTCCAAAAGC GGCCCCAAGG CCTTAGCTTC CTTGGCCTGT CAGGCTGCGC CGTCCAGGGT 
CCCCTGGGCC TGGGCCTGTG GGACAAGATA GCCGCGCAGC TCCGGGAACT GCAGCTGTGC 
AGCAGACGCC TCTGCGCTGA GGACAGGGAC GCCCTGCGCC AGCTGCAGCC CAGTCGGCCG 
GGCCCCGGCG AGTGCACGCT GGACCACGGC TCCAAGCTCT TCTTTCGGCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038460.4
CDS	16..4152
Translation	MSLERELRQLSKAKAKAQRAGQRREEAALCHQLGELLAGHGRYAEALEQHWQELQLRERADDPLGCAVAHRKIGERLAEMEDYPAALQHQHQYLELAHSLRNHTELQRAWATIGRTHLDIYDHCQSRDALLQAQAAFEKSLAIVDEELEGTLAQGELNEMRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARYNLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFLAAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQAHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPGPGECTLDHGSKLFFRRL

Target ORF information:

RefSeq Version	NM_013432.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013432.4

ORF Insert Sequence:

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGGTCC GGCCCCGCCC CCTCCCATCC GGGTTCGAGT TCAAGTTCAG 
GATCATCTCT TCCTCATCCC TGTCCCACAC AGCAGTGACA CCCACTCTGT GGCCTGGCTG 
GCCGAGCAGG CGGCCCAGCG CTACTACCAG ACCTGCGGGC TGCTGCCCAG GCTCACCCTA 
CGGAAAGAGG GGGCCCTGCT GGCCCCACAG GACCTCATCC CTGATGTGCT GCAGAGCAAT 
GACGAGGTGT TGGCTGAGGT GACTTCGTGG GACCTGCCCC CGTTGACTGA CCGCTACCGC 
AGGGCCTGCC AGAGCCTGGG GCAAGGGGAG CACCAACAGG TGCTGCAGGC CGTGGAGCTC 
CAGGGCTTGG GCCTCTCGTT CAGCGCCTGC TCCCTGGCCC TGGACCAGGC CCAGCTTACA 
CCCCTGCTGC GGGCCCTCAA GCTGCACACA GCACTCCGGG AGCTGCGCCT GGCAGGGAAC 
CGGCTGGGGG ACAAGTGTGT GGCTGAGCTG GTGGCTGCCC TGGGCACCAT GCCCAGCCTG 
GCCCTCCTTG ACCTCTCCTC CAATCACCTG GGTCCCGAAG GCCTGCGCCA GCTTGCCATG 
GGGCTCCCAG GCCAAGCCAC CTTGCAGAGT TTGGAGGAGC TGGACTTAAG CATGAACCCC 
CTGGGGGACG GCTGTGGCCA GTCCCTGGCC TCCCTCCTGC ACGCCTGCCC CTTACTCAGC 
ACCCTGCGCC TGCAGGCGTG TGGCTTCGGC CCCAGCTTCT TTCTGAGCCA CCAGACAGCA 
CTGGGTAGTG CTTTCCAAGA TGCTGAGCAC CTGAAGACCC TGTCCCTGTC CTACAACGCC 
CTGGGAGCCC CTGCCCTGGC CAGGACCCTG CAGAGCCTGC CCGCCGGCAC CCTCCTGCAC 
TTAGAGCTCA GCTCCGTGGC AGCCGGCAAG GGTGATTCGG ACCTCATGGA GCCTGTATTC 
CGATACCTGG CCAAGGAAGG CTGTGCTCTA GCCCACCTGA CCCTGTCTGC AAACCACCTG 
GGGGACAAGG CTGTTAGAGA CCTGTGCAGA TGTCTCTCTC TGTGCCCCTC ACTCATCTCA 
CTGGATCTGT CTGCCAACCC TGAGATCAGC TGTGCCAGCT TGGAAGAGCT CCTGTCCACC 
CTCCAAAAGC GGCCCCAAGG CCTTAGCTTC CTTGGCCTGT CAGGCTGCGC CGTCCAGGGT 
CCCCTGGGCC TGGGCCTGTG GGACAAGATA GCCGCGCAGC TCCGGGAACT GCAGCTGTGC 
AGCAGACGCC TCTGCGCTGA GGACAGGGAC GCCCTGCGCC AGCTGCAGCC CAGTCGGCCG 
GGCCCCGGCG AGTGCACGCT GGACCACGGC TCCAAGCTCT TCTTTCGGCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TONSL ( NM_013432.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_038460.4 Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.

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CloneID	OHu32001
Clone ID Related Accession (Same CDS sequence)	NM_013432.4 , NM_013432.5
Accession Version	NM_013432.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	tonsoku-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084125.9, CN366457.1, BC008782.2, BF475381.1 and AC233992.5. On Nov 22, 2018 this sequence version replaced NM_013432.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008782.2, SRR1803616.59587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000409379.8/ ENSP00000386239.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGGTCC GGCCCCGCCC CCTCCCATCC GGGTTCGAGT TCAAGTTCAG 
GATCATCTCT TCCTCATCCC TGTCCCACAC AGCAGTGACA CCCACTCTGT GGCCTGGCTG 
GCCGAGCAGG CGGCCCAGCG CTACTACCAG ACCTGCGGGC TGCTGCCCAG GCTCACCCTA 
CGGAAAGAGG GGGCCCTGCT GGCCCCACAG GACCTCATCC CTGATGTGCT GCAGAGCAAT 
GACGAGGTGT TGGCTGAGGT GACTTCGTGG GACCTGCCCC CGTTGACTGA CCGCTACCGC 
AGGGCCTGCC AGAGCCTGGG GCAAGGGGAG CACCAACAGG TGCTGCAGGC CGTGGAGCTC 
CAGGGCTTGG GCCTCTCGTT CAGCGCCTGC TCCCTGGCCC TGGACCAGGC CCAGCTTACA 
CCCCTGCTGC GGGCCCTCAA GCTGCACACA GCACTCCGGG AGCTGCGCCT GGCAGGGAAC 
CGGCTGGGGG ACAAGTGTGT GGCTGAGCTG GTGGCTGCCC TGGGCACCAT GCCCAGCCTG 
GCCCTCCTTG ACCTCTCCTC CAATCACCTG GGTCCCGAAG GCCTGCGCCA GCTTGCCATG 
GGGCTCCCAG GCCAAGCCAC CTTGCAGAGT TTGGAGGAGC TGGACTTAAG CATGAACCCC 
CTGGGGGACG GCTGTGGCCA GTCCCTGGCC TCCCTCCTGC ACGCCTGCCC CTTACTCAGC 
ACCCTGCGCC TGCAGGCGTG TGGCTTCGGC CCCAGCTTCT TTCTGAGCCA CCAGACAGCA 
CTGGGTAGTG CTTTCCAAGA TGCTGAGCAC CTGAAGACCC TGTCCCTGTC CTACAACGCC 
CTGGGAGCCC CTGCCCTGGC CAGGACCCTG CAGAGCCTGC CCGCCGGCAC CCTCCTGCAC 
TTAGAGCTCA GCTCCGTGGC AGCCGGCAAG GGTGATTCGG ACCTCATGGA GCCTGTATTC 
CGATACCTGG CCAAGGAAGG CTGTGCTCTA GCCCACCTGA CCCTGTCTGC AAACCACCTG 
GGGGACAAGG CTGTTAGAGA CCTGTGCAGA TGTCTCTCTC TGTGCCCCTC ACTCATCTCA 
CTGGATCTGT CTGCCAACCC TGAGATCAGC TGTGCCAGCT TGGAAGAGCT CCTGTCCACC 
CTCCAAAAGC GGCCCCAAGG CCTTAGCTTC CTTGGCCTGT CAGGCTGCGC CGTCCAGGGT 
CCCCTGGGCC TGGGCCTGTG GGACAAGATA GCCGCGCAGC TCCGGGAACT GCAGCTGTGC 
AGCAGACGCC TCTGCGCTGA GGACAGGGAC GCCCTGCGCC AGCTGCAGCC CAGTCGGCCG 
GGCCCCGGCG AGTGCACGCT GGACCACGGC TCCAAGCTCT TCTTTCGGCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038460.4
CDS	27..4163
Translation	MSLERELRQLSKAKAKAQRAGQRREEAALCHQLGELLAGHGRYAEALEQHWQELQLRERADDPLGCAVAHRKIGERLAEMEDYPAALQHQHQYLELAHSLRNHTELQRAWATIGRTHLDIYDHCQSRDALLQAQAAFEKSLAIVDEELEGTLAQGELNEMRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARYNLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFLAAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQAHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPGPGECTLDHGSKLFFRRL

Target ORF information:

RefSeq Version	NM_013432.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tonsoku like, DNA repair protein (TONSL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013432.5

ORF Insert Sequence:

ATGAGCCTGG AGCGCGAGCT TCGCCAGCTG AGCAAGGCGA AAGCCAAGGC GCAGAGGGCC 
GGGCAGCGGC GCGAAGAGGC CGCGCTGTGC CACCAGCTGG GGGAGCTCCT GGCCGGCCAT 
GGCCGCTACG CCGAGGCTCT GGAGCAGCAC TGGCAGGAGC TGCAGCTTCG GGAGCGCGCT 
GACGACCCTC TGGGCTGTGC CGTGGCCCAC CGCAAGATCG GAGAGCGCCT GGCCGAGATG 
GAGGACTACC CGGCTGCCTT GCAGCACCAG CACCAGTACC TGGAGCTGGC ACATTCCCTG 
CGCAACCACA CGGAGCTGCA GAGGGCCTGG GCCACCATCG GCCGCACCCA CCTGGACATC 
TATGACCACT GCCAGTCGAG GGATGCTTTG CTGCAGGCAC AGGCTGCCTT TGAGAAGAGC 
TTGGCTATTG TGGATGAGGA GCTGGAGGGG ACACTGGCCC AGGGAGAGCT GAATGAGATG 
AGGACCCGCC TCTATCTCAA CCTGGGCCTC ACCTTTGAGA GCCTGCAGCA GACAGCCCTG 
TGCAACGATT ACTTCAGGAA GAGCATCTTC CTTGCGGAGC AGAACCACCT TTACGAGGAC 
CTATTCCGCG CCCGCTACAA CCTGGGCACC ATCCACTGGC GCGCGGGCCA GCACTCCCAG 
GCTATGCGCT GCTTGGAGGG TGCCCGGGAG TGTGCGCACA CCATGAGGAA GCGGTTCATG 
GAGAGCGAGT GCTGCGTGGT TATTGCACAG GTCCTCCAAG ACCTGGGAGA CTTTTTGGCT 
GCCAAGCGAG CCCTGAAGAA GGCCTACAGG CTGGGCTCCC AGAAGCCTGT GCAGAGGGCA 
GCCATCTGTC AGAACCTCCA GCATGTGCTG GCAGTGGTCC GGCTGCAGCA ACAGCTGGAA 
GAGGCTGAGG GCAGAGACCC TCAGGGTGCC ATGGTCATCT GTGAGCAGCT AGGGGACCTC 
TTCTCCAAGG CAGGAGACTT TCCCAGGGCA GCTGAGGCTT ACCAGAAGCA GCTGCGTTTT 
GCTGAGCTGC TGGACAGACC GGGTGCTGAG CGGGCCATCA TCCACGTGTC CCTGGCCACC 
ACACTGGGAG ACATGAAGGA CCACCATGGG GCCGTGCGCC ACTATGAGGA GGAACTGAGG 
CTGCGCAGCG GCAACGTGCT GGAGGAGGCC AAGACCTGGC TGAACATTGC ACTGTCCCGC 
GAGGAGGCCG GCGATGCCTA CGAGCTGCTG GCCCCGTGCT TCCAGAAAGC GCTCAGCTGT 
GCCCAGCAGG CCCAGCGTCC CCAGCTGCAG AGGCAGGTCT TGCAGCATCT CCATACCGTG 
CAGCTGAGGC TGCAGCCCCA GGAGGCCCCT GAGACCGAAA CCAGACTACG GGAGCTCAGT 
GTAGCTGAAG ATGAAGATGA GGAGGAGGAG GCGGAGGAGG CGGCAGCCAC AGCGGAGAGC 
GAAGCCCTGG AGGCCGGCGA GGTGGAGCTC TCAGAGGGCG AGGACGACAC CGATGGCCTG 
ACCCCGCAGC TGGAGGAGGA CGAGGAGCTT CAGGGCCACC TGGGCCGGCG GAAGGGGAGC 
AAGTGGAACC GGCGAAACGA CATGGGGGAG ACCCTGCTGC ACCGAGCCTG CATCGAGGGC 
CAGCTGCGCC GCGTCCAGGA CCTTGTGAGG CAGGGCCACC CCCTTAACCC TCGGGACTAC 
TGTGGCTGGA CACCTCTGCA CGAGGCCTGC AACTACGGGC ATCTAGAAAT TGTCCGCTTC 
CTGCTGGACC ACGGGGCCGC AGTGGACGAC CCAGGTGGCC AGGGCTGCGA AGGCATCACC 
CCCCTCCACG ATGCCCTCAA CTGTGGCCAC TTCGAGGTGG CTGAGCTGCT GCTTGAACGG 
GGGGCGTCCG TCACCCTCCG CACTCGAAAG GGCCTCAGCC CGCTGGAGAC GCTGCAGCAG 
TGGGTGAAGC TGTACCGCAG GGACCTGGAC CTGGAGACGC GGCAGAAGGC CAGGGCCATG 
GAGATGCTGC TCCAGGCGGC TGCCTCGGGC CAAGATCCCC ACAGCTCCCA GGCCTTCCAC 
ACCCCAAGCA GCCTTCTGTT TGACCCCGAG ACCTCTCCTC CTTTGAGCCC CTGCCCAGAA 
CCCCCCTCTA ATAGCACTAG ACTCCCAGAG GCCTCTCAGG CCCATGTCAG GGTCTCCCCA 
GGGCAGGCGG CACCAGCCAT GGCCAGGCCT CGGAGGAGCA GGCATGGGCC AGCCAGCAGC 
AGCAGCAGCT CAGAAGGCGA GGACAGCGCA GGCCCCGCAC GGCCGTCCCA GAAGAGGCCT 
CGGTGCTCGG CCACAGCACA ACGGGTGGCA GCCTGGACGC CTGGCCCCGC CAGCAACAGG 
GAAGCAGCCA CAGCCAGCAC CAGCCGGGCA GCCTACCAGG CAGCCATCCG GGGTGTGGGC 
AGTGCTCAGA GCCGGCTGGG GCCTGGCCCA CCGCGGGGCC ACAGCAAAGC CCTTGCCCCC 
CAGGCAGCGC TCATCCCGGA GGAGGAGTGC CTGGCCGGGG ACTGGCTGGA GCTGGACATG 
CCCCTGACCC GCAGCCGCCG GCCCCGCCCC CGGGGCACTG GAGACAACCG CAGGCCCAGT 
AGTACCTCTG GGTCGGACAG TGAGGAGAGC AGGCCCCGTG CCCGAGCCAA GCAGGTCCGC 
CTGACCTGCA TGCAGAGTTG CAGTGCGCCA GTTAACGCAG GGCCCAGCAG CCTGGCTTCA 
GAACCTCCAG GGAGCCCCAG CACCCCCAGG GTCTCAGAGC CCAGTGGGGA CAGCTCTGCG 
GCAGGCCAGC CCTTGGGTCC GGCCCCGCCC CCTCCCATCC GGGTTCGAGT TCAAGTTCAG 
GATCATCTCT TCCTCATCCC TGTCCCACAC AGCAGTGACA CCCACTCTGT GGCCTGGCTG 
GCCGAGCAGG CGGCCCAGCG CTACTACCAG ACCTGCGGGC TGCTGCCCAG GCTCACCCTA 
CGGAAAGAGG GGGCCCTGCT GGCCCCACAG GACCTCATCC CTGATGTGCT GCAGAGCAAT 
GACGAGGTGT TGGCTGAGGT GACTTCGTGG GACCTGCCCC CGTTGACTGA CCGCTACCGC 
AGGGCCTGCC AGAGCCTGGG GCAAGGGGAG CACCAACAGG TGCTGCAGGC CGTGGAGCTC 
CAGGGCTTGG GCCTCTCGTT CAGCGCCTGC TCCCTGGCCC TGGACCAGGC CCAGCTTACA 
CCCCTGCTGC GGGCCCTCAA GCTGCACACA GCACTCCGGG AGCTGCGCCT GGCAGGGAAC 
CGGCTGGGGG ACAAGTGTGT GGCTGAGCTG GTGGCTGCCC TGGGCACCAT GCCCAGCCTG 
GCCCTCCTTG ACCTCTCCTC CAATCACCTG GGTCCCGAAG GCCTGCGCCA GCTTGCCATG 
GGGCTCCCAG GCCAAGCCAC CTTGCAGAGT TTGGAGGAGC TGGACTTAAG CATGAACCCC 
CTGGGGGACG GCTGTGGCCA GTCCCTGGCC TCCCTCCTGC ACGCCTGCCC CTTACTCAGC 
ACCCTGCGCC TGCAGGCGTG TGGCTTCGGC CCCAGCTTCT TTCTGAGCCA CCAGACAGCA 
CTGGGTAGTG CTTTCCAAGA TGCTGAGCAC CTGAAGACCC TGTCCCTGTC CTACAACGCC 
CTGGGAGCCC CTGCCCTGGC CAGGACCCTG CAGAGCCTGC CCGCCGGCAC CCTCCTGCAC 
TTAGAGCTCA GCTCCGTGGC AGCCGGCAAG GGTGATTCGG ACCTCATGGA GCCTGTATTC 
CGATACCTGG CCAAGGAAGG CTGTGCTCTA GCCCACCTGA CCCTGTCTGC AAACCACCTG 
GGGGACAAGG CTGTTAGAGA CCTGTGCAGA TGTCTCTCTC TGTGCCCCTC ACTCATCTCA 
CTGGATCTGT CTGCCAACCC TGAGATCAGC TGTGCCAGCT TGGAAGAGCT CCTGTCCACC 
CTCCAAAAGC GGCCCCAAGG CCTTAGCTTC CTTGGCCTGT CAGGCTGCGC CGTCCAGGGT 
CCCCTGGGCC TGGGCCTGTG GGACAAGATA GCCGCGCAGC TCCGGGAACT GCAGCTGTGC 
AGCAGACGCC TCTGCGCTGA GGACAGGGAC GCCCTGCGCC AGCTGCAGCC CAGTCGGCCG 
GGCCCCGGCG AGTGCACGCT GGACCACGGC TCCAAGCTCT TCTTTCGGCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The MMS22L-TONSL heterodimer directly promotes RAD51-dependent recombination upon replication stress.
The EMBO journal35(23)2584-2601(2016 12)
Piwko W,Mlejnkova LJ,Mutreja K,Ranjha L,Stafa D,Smirnov A,Brodersen MM,Zellweger R,Sturzenegger A,Janscak P,Lopes M,Peter M,Cejka P

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TONSL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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