NFE2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFE2
Entrez Gene ID	4778
Full Name	nuclear factor, erythroid 2
Synonyms	NF-E2,p45
General protein information	Preferred Names nuclear factor, erythroid 2 Names transcription factor NF-E2 45 kDa subunit leucine zipper protein NF-E2 nuclear factor (erythroid-derived 2), 45kD nuclear factor (erythroid-derived 2), 45kDa nuclear factor, erythroid-derived 2 45 kDa subunit p45 NF-E2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Disorder MIM:	601490

mRNA and Protein(s)

mRNA	Protein	Name
XM_005268906.3	XP_005268963.1	transcription factor NF-E2 45 kDa subunit isoform X1
NM_001136023.2	NP_001129495.1	transcription factor NF-E2 45 kDa subunit
NM_006163.2	NP_006154.1	transcription factor NF-E2 45 kDa subunit
NM_001136023.3	NP_001129495.1	transcription factor NF-E2 45 kDa subunit
XM_005268906.4	XP_005268963.1	transcription factor NF-E2 45 kDa subunit isoform X1
NM_001261461.1	NP_001248390.1	transcription factor NF-E2 45 kDa subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFE2	XP_001170885.1
Mus musculus (house mouse)	Nfe2	NP_032711.2
Rattus norvegicus (Norway rat)	Nfe2	NP_001012224.1
Xenopus tropicalis (tropical clawed frog)	nfe2	NP_001123389.1
	NFE2	XP_002798659.1
Canis lupus familiaris (dog)	NFE2	XP_005636802.1
Homo sapiens (human)	NFE2	NP_006154.1
Bos taurus (cattle)	NFE2	NP_001014923.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear Factor Erythroid 2 Regulates Human HSC Self-Renewal and T Cell Differentiation by Preventing NOTCH1 Activation.
Di Tullio A, Passaro D, Rouault-Pierre K, Purewal S, Bonnet D
Stem cell reports9(1)5-11(2017 Jul)

NFE2 Induces miR-423-5p to Promote Gluconeogenesis and Hyperglycemia by Repressing the Hepatic FAM3A-ATP-Akt Pathway.
Yang W, Wang J, Chen Z, Chen J, Meng Y, Chen L, Chang Y, Geng B, Sun L, Dou L, Li J, Guan Y, Cui Q, Yang J
Diabetes66(7)1819-1832(2017 07)

p45 NF-E2 regulates syncytiotrophoblast differentiation by post-translational GCM1 modifications in human intrauterine growth restriction.
Kohli S, Hoffmann J, Lochmann F, Markmeyer P, Huebner H, Fahlbusch FB, Al-Dabet MM, Gadi I, Manoharan J, L?ttge M, Zenclussen AC, Aharon A, Brenner B, Shahzad K, Ruebner M, Isermann B
Cell death & disease8(4)e2730(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

under the obese condition, activation of the hepatic NFE2/miR-423-5p axis plays important roles in the progression of type 2 diabetes and NAFLD by repressing the FAM3A-ATP-Akt signaling pathway.
Title: NFE2 Induces miR-423-5p to Promote Gluconeogenesis and Hyperglycemia by Repressing the Hepatic FAM3A-ATP-Akt Pathway.

results suggest that p45 NF-E2 negatively regulates differentiation and apoptosis activation of human syncytiotrophoblast by modulating GCM1 acetylation and sumoylation.
Title: p45 NF-E2 regulates syncytiotrophoblast differentiation by post-translational GCM1 modifications in human intrauterine growth restriction.

Our data presented herein support the hypothesis that cytoplasmically located NF-E2 may have a function in the elimination of internal organelles. The mild autophagy defect observed in our mice may be due to the requirement for additional proteins in the formation of larger complexes to exert this function.
Title: A novel role for nuclear factor-erythroid 2 in erythroid maturation by modulation of mitochondrial autophagy.

NF-E2, TAL1 and KLF1, all activators play a primary role in HSs formation in the LCR
Title: Erythroid activator NF-E2, TAL1 and KLF1 play roles in forming the LCR HSs in the human adult ?-globin locus.

assessment of NF-E2 and NGFR expression may provide additional support in reaching a correct diagnosis in discriminating polycythemia vera from prefibrotic/fibrotic primary myelofibrosis and essential thrombocythemia
Title: Nuclear factor-erythroid 2, nerve growth factor receptor, and CD34-microvessel density are differentially expressed in primary myelofibrosis, polycythemia vera, and essential thrombocythemia.

The following NFE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19898	NM_001261461.1 Latest version!	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19898	NM_001136023.3 Latest version!	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19898	NM_006163.2 Latest version!	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19898	XM_005268906.4 Latest version!	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu19898	NM_001136023.2	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19898	XM_005268906.3	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFE2 ( NM_001136023.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129495.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	NM_001136023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	transcription factor NF-E2 45 kDa subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE243330.1, BC005044.1 and GD141614.1. On May 16, 2012 this sequence version replaced NM_001136023.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA846544.1, BC005044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129495.1
CDS	294..1415
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	NM_001136023.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136023.2

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE2 ( NM_006163.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006154.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	NM_006163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	transcription factor NF-E2 45 kDa subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX406317.2, S77763.1 and GD141614.1. On May 17, 2012 this sequence version replaced NM_006163.1. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX406317.2, S77763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006154.1
CDS	293..1414
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	NM_006163.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006163.2

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE2 ( NM_001136023.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129495.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	NM_001136023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	transcription factor NF-E2 45 kDa subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE243330.1 and BC005044.1. On Nov 24, 2018 this sequence version replaced NM_001136023.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.104564.1, SRR5189661.64803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000435572.7/ ENSP00000397185.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129495.1
CDS	272..1393
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	NM_001136023.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136023.3

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE2 ( NM_001261461.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001248390.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 3, mRNA.

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CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	NM_001261461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	transcription factor NF-E2 45 kDa subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE243330.1, AK313207.1 and GD141614.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313207.1, DA674455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001248390.1
CDS	424..1545
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	NM_001261461.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001261461.1

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE2 ( XM_005268906.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268963.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.

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CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	XM_005268906.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	transcription factor NF-E2 45 kDa subunit isoform X1
Comment

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268963.1
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	XM_005268906.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268906.3

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE2 ( XM_005268906.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268963.1 Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19898
Clone ID Related Accession (Same CDS sequence)	XM_005268906.3 , NM_001136023.2 , NM_006163.2 , NM_001136023.3 , XM_005268906.4 , NM_001261461.1
Accession Version	XM_005268906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor NF-E2 45 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268963.1
CDS	144..1265
Translation	MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

Target ORF information:

RefSeq Version	XM_005268906.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 2 (NFE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268906.4

ORF Insert Sequence:

ATGTCCCCGT GTCCTCCCCA GCAGAGCAGG AACAGGGTGA TACAGCTGTC CACTTCAGAG 
CTAGGAGAGA TGGAACTGAC TTGGCAGGAG ATCATGTCCA TCACCGAGCT GCAGGGTCTG 
AATGCTCCAA GTGAGCCATC ATTTGAGCCC CAAGCCCCAG CTCCATACCT TGGACCTCCA 
CCACCCACAA CTTACTGCCC CTGCTCAATC CACCCAGATT CTGGCTTCCC ACTTCCTCCA 
CCACCTTATG AGCTCCCAGC ATCCACATCC CATGTCCCAG ATCCCCCATA CTCCTATGGC 
AACATGGCCA TACCAGTCTC CAAGCCACTG AGCCTCTCAG GCCTGCTCAG TGAGCCGCTC 
CAAGACCCCT TAGCCCTCCT GGACATTGGG CTGCCAGCAG GGCCACCTAA GCCCCAAGAA 
GACCCAGAAT CCGACTCAGG ATTATCCCTC AACTATAGCG ATGCTGAATC TCTTGAGCTG 
GAGGGGACAG AGGCTGGTCG GCGGCGCAGC GAATATGTAG AGATGTACCC AGTGGAGTAC 
CCCTACTCAC TCATGCCCAA CTCCTTGGCC CACTCCAACT ATACCTTGCC AGCTGCTGAG 
ACCCCCTTGG CCTTAGAGCC CTCCTCAGGC CCTGTGCGGG CTAAGCCCAC TGCACGGGGG 
GAGGCAGGGA GTCGGGATGA ACGTCGGGCC TTGGCCATGA AGATTCCTTT TCCTACGGAC 
AAGATTGTCA ACTTGCCGGT AGATGACTTT AATGAGCTAT TGGCAAGGTA CCCGCTGACA 
GAGAGCCAGC TAGCGCTAGT CCGGGACATC CGACGACGGG GCAAAAACAA GGTGGCAGCC 
CAGAACTGCC GCAAGAGGAA GCTGGAAACC ATTGTGCAGC TGGAGCGGGA GCTGGAGCGG 
CTGACCAATG AACGGGAGCG GCTTCTCAGG GCCCGCGGGG AGGCAGACCG GACCCTGGAG 
GTCATGCGCC AACAGCTGAC AGAGCTGTAC CGTGACATTT TCCAGCACCT TCGGGATGAA 
TCAGGCAACA GCTACTCTCC TGAAGAGTAC GCGCTGCAAC AGGCTGCCGA TGGGACCATC 
TTCCTTGTGC CCCGGGGGAC CAAGATGGAG GCCACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear Factor Erythroid 2 Regulates Human HSC Self-Renewal and T Cell Differentiation by Preventing NOTCH1 Activation.
Stem cell reports9(1)5-11(2017 Jul)
Di Tullio A,Passaro D,Rouault-Pierre K,Purewal S,Bonnet D

NFE2 Induces miR-423-5p to Promote Gluconeogenesis and Hyperglycemia by Repressing the Hepatic FAM3A-ATP-Akt Pathway.
Diabetes66(7)1819-1832(2017 07)
Yang W,Wang J,Chen Z,Chen J,Meng Y,Chen L,Chang Y,Geng B,Sun L,Dou L,Li J,Guan Y,Cui Q,Yang J

p45 NF-E2 regulates syncytiotrophoblast differentiation by post-translational GCM1 modifications in human intrauterine growth restriction.
Cell death & disease8(4)e2730(2017 Apr)
Kohli S,Hoffmann J,Lochmann F,Markmeyer P,Huebner H,Fahlbusch FB,Al-Dabet MM,Gadi I,Manoharan J,L?ttge M,Zenclussen AC,Aharon A,Brenner B,Shahzad K,Ruebner M,Isermann B

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NFE2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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