ATOX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATOX1
Entrez Gene ID	475
Full Name	antioxidant 1 copper chaperone
Synonyms	ATX1,HAH1
General protein information	Preferred Names antioxidant 1 copper chaperone Names copper transport protein ATOX1 ATX1 antioxidant protein 1 homolog metal transport protein ATX1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q33.1
Summary	This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	602270

mRNA and Protein(s)

mRNA	Protein	Name
NM_004045.3	NP_004036.1	copper transport protein ATOX1
NM_004045.4	NP_004036.1	copper transport protein ATOX1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATOX1	NP_001003119.1
Bos taurus (cattle)	ATOX1	NP_001124230.1
	LOC712464	XP_001101466.2
Danio rerio (zebrafish)	atox1	NP_001243562.1
Homo sapiens (human)	ATOX1	NP_004036.1
Mus musculus (house mouse)	Atox1	NP_033850.1
Xenopus tropicalis (tropical clawed frog)	atox1	NP_001039238.1
Pan troglodytes (chimpanzee)	ATOX1	XP_001169183.1
Rattus norvegicus (Norway rat)	Atox1	NP_445811.1
Gallus gallus (chicken)	ATOX1	NP_001264641.1

Gene Ontology
Bibliography

Related articles in PubMed

Copper chaperone antioxidant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells.
Kamiya T, Takeuchi K, Fukudome S, Hara H, Adachi T
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine31(1)61-68(2018 Feb)

The metal chaperone Atox1 regulates the activity of the human copper transporter ATP7B by modulating domain dynamics.
Yu CH, Yang N, Bothe J, Tonelli M, Nokhrin S, Dolgova NV, Braiterman L, Lutsenko S, Dmitriev OY
The Journal of biological chemistry292(44)18169-18177(2017 11)

The structural flexibility of the human copper chaperone Atox1: Insights from combined pulsed EPR studies and computations.
Levy AR, Turgeman M, Gevorkyan-Aiapetov L, Ruthstein S
Protein science : a publication of the Protein Society26(8)1609-1618(2017 Aug)

Binding of Copper and Cisplatin to Atox1 Is Mediated by Glutathione through the Formation of Metal-Sulfur Clusters.
Dolgova NV, Yu C, Cvitkovic JP, Hodak M, Nienaber KH, Summers KL, Cotelesage JJH, Bernholc J, Kaminski GA, Pickering IJ, George GN, Dmitriev OY
Biochemistry56(24)3129-3141(2017 06)

Thiol-based copper handling by the copper chaperone Atox1.
Hatori Y, Inouye S, Akagi R
IUBMB life69(4)246-254(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that N-terminal segment of metal-binding domains (MBDs) 1-3 of ATOX1 interact with nucleotide-binding domain of ATP7B, thus physically coupling the domains involved in copper binding and those involved in ATP hydrolysis; interactions with MBDs 1-3 of ATOX1 activate ATP7B ATP hydrolysis. (ATOX1 = copper transport protein ATOX1; ATP7B = Cu-binding P type ATPase ATP7B)
Title: The metal chaperone Atox1 regulates the activity of the human copper transporter ATP7B by modulating domain dynamics.

The structural flexibility of the human copper chaperone Atox1 has been reported based on insights from combined pulsed EPR studies and computations.
Title: The structural flexibility of the human copper chaperone Atox1: Insights from combined pulsed EPR studies and computations.

It show that copper(I) and glutathione form large polymers with a molecular mass of approximately 8 kDa, which can transfer copper to Atox1.
Title: Binding of Copper and Cisplatin to Atox1 Is Mediated by Glutathione through the Formation of Metal-Sulfur Clusters.

Highlighted in this review are unique redox properties of Atox1 and other copper chaperones. Also, summarized are the redox nodes in the cytosol which potentially play dominant roles in the redox regulation of copper chaperones
Title: Thiol-based copper handling by the copper chaperone Atox1.

Cu chaperone Atox1 has a role in breast cancer cell migration
Title: Copper chaperone Atox1 plays role in breast cancer cell migration.

The following ATOX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATOX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08975	NM_004045.4 Latest version!	Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu08975	NM_004045.3	Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATOX1 ( NM_004045.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004036.1 Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.

Price & Availability↑

CloneID	OHu08975
Clone ID Related Accession (Same CDS sequence)	NM_004045.3 , NM_004045.4
Accession Version	NM_004045.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	copper transport protein ATOX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599233.1 and CN280769.1. On Aug 9, 2005 this sequence version replaced NM_004045.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI598370.1, BI599233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGAAGC ACGAGTTCTC TGTGGACATG ACCTGTGGAG GCTGTGCTGA AGCTGTCTCT 
CGGGTCCTCA ATAAGCTTGG AGGAGTTAAG TATGACATTG ACCTGCCCAA CAAGAAGGTC 
TGCATTGAAT CTGAGCACAG CATGGACACT CTGCTTGCAA CCCTGAAGAA AACAGGAAAG 
ACTGTTTCCT ACCTTGGCCT TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004036.1
CDS	99..305
Translation	MPKHEFSVDMTCGGCAEAVSRVLNKLGGVKYDIDLPNKKVCIESEHSMDTLLATLKKTGKTVSYLGLE

Target ORF information:

RefSeq Version	NM_004045.3
Organism	Homo sapiens(human)
Definition	Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004045.3

ORF Insert Sequence:

ATGCCGAAGC ACGAGTTCTC TGTGGACATG ACCTGTGGAG GCTGTGCTGA AGCTGTCTCT 
CGGGTCCTCA ATAAGCTTGG AGGAGTTAAG TATGACATTG ACCTGCCCAA CAAGAAGGTC 
TGCATTGAAT CTGAGCACAG CATGGACACT CTGCTTGCAA CCCTGAAGAA AACAGGAAAG 
ACTGTTTCCT ACCTTGGCCT TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATOX1 ( NM_004045.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004036.1 Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.

Price & Availability↑

CloneID	OHu08975
Clone ID Related Accession (Same CDS sequence)	NM_004045.3 , NM_004045.4
Accession Version	NM_004045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	copper transport protein ATOX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599233.1 and CN280769.1. On Nov 22, 2018 this sequence version replaced NM_004045.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI598370.1, BI599233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000313115.11/ ENSP00000316854.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGAAGC ACGAGTTCTC TGTGGACATG ACCTGTGGAG GCTGTGCTGA AGCTGTCTCT 
CGGGTCCTCA ATAAGCTTGG AGGAGTTAAG TATGACATTG ACCTGCCCAA CAAGAAGGTC 
TGCATTGAAT CTGAGCACAG CATGGACACT CTGCTTGCAA CCCTGAAGAA AACAGGAAAG 
ACTGTTTCCT ACCTTGGCCT TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004036.1
CDS	81..287
Translation	MPKHEFSVDMTCGGCAEAVSRVLNKLGGVKYDIDLPNKKVCIESEHSMDTLLATLKKTGKTVSYLGLE

Target ORF information:

RefSeq Version	NM_004045.4
Organism	Homo sapiens(human)
Definition	Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004045.4

ORF Insert Sequence:

ATGCCGAAGC ACGAGTTCTC TGTGGACATG ACCTGTGGAG GCTGTGCTGA AGCTGTCTCT 
CGGGTCCTCA ATAAGCTTGG AGGAGTTAAG TATGACATTG ACCTGCCCAA CAAGAAGGTC 
TGCATTGAAT CTGAGCACAG CATGGACACT CTGCTTGCAA CCCTGAAGAA AACAGGAAAG 
ACTGTTTCCT ACCTTGGCCT TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Copper chaperone antioxidant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine31(1)61-68(2018 Feb)
Kamiya T,Takeuchi K,Fukudome S,Hara H,Adachi T

The metal chaperone Atox1 regulates the activity of the human copper transporter ATP7B by modulating domain dynamics.
The Journal of biological chemistry292(44)18169-18177(2017 11)
Yu CH,Yang N,Bothe J,Tonelli M,Nokhrin S,Dolgova NV,Braiterman L,Lutsenko S,Dmitriev OY

The structural flexibility of the human copper chaperone Atox1: Insights from combined pulsed EPR studies and computations.
Protein science : a publication of the Protein Society26(8)1609-1618(2017 Aug)
Levy AR,Turgeman M,Gevorkyan-Aiapetov L,Ruthstein S

Binding of Copper and Cisplatin to Atox1 Is Mediated by Glutathione through the Formation of Metal-Sulfur Clusters.
Biochemistry56(24)3129-3141(2017 06)
Dolgova NV,Yu C,Cvitkovic JP,Hodak M,Nienaber KH,Summers KL,Cotelesage JJH,Bernholc J,Kaminski GA,Pickering IJ,George GN,Dmitriev OY

Thiol-based copper handling by the copper chaperone Atox1.
IUBMB life69(4)246-254(2017 Apr)
Hatori Y,Inouye S,Akagi R

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ATOX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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