NDUFA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFA1
Entrez Gene ID	4694
Full Name	NADH:ubiquinone oxidoreductase subunit A1
Synonyms	CI-MWFE,MWFE,ZNF183
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit A1 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa NADH-ubiquinone oxidoreductase MWFE subunit NADH:ubiquinone oxidoreductase (complex 1) complex I MWFE subunit type I dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq24
Summary	The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the 'hydrophobic protein' (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008].
Disorder MIM:	300078
Disorder Html:	Mitochondrial complex I deficiency, 252010 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004541.4	NP_004532.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
NM_004541.3	NP_004532.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Pathways from BioSystems

Homologies

	NDUFA1	XP_001109409.1
Mus musculus (house mouse)	Ndufa1	NP_062316.1
Homo sapiens (human)	NDUFA1	NP_004532.1
	NDUFA1	XP_001083658.2
	LOC710709	XP_001099376.2
Bos taurus (cattle)	NDUFA1	NP_786988.1
Danio rerio (zebrafish)	ndufa1	NP_001161327.1
Bos taurus (cattle)	LOC101904690	XP_005228018.1
Rattus norvegicus (Norway rat)	Ndufa1	NP_001102283.2
Gallus gallus (chicken)	NDUFA1	XP_001234262.1
Pan troglodytes (chimpanzee)	NDUFA1	XP_003317703.1
Canis lupus familiaris (dog)	LOC102154035	XP_005641801.1
Canis lupus familiaris (dog)	NDUFA1	XP_005630839.1
Xenopus tropicalis (tropical clawed frog)	ndufa1	NP_001165138.1

Gene Ontology
Bibliography

Related articles in PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Altered mitochondrial expression genes in patients receiving right ventricular apical pacing.
Xu H, Gao J, Wang F
Experimental and molecular pathology100(3)469-75(2016 06)

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrent? L, Mar JC
PLoS genetics11(8)e1005428(2015 Aug)

Mitochondrial respiratory chain Complex I defects in Fanconi anemia complementation group A.
Ravera S, Vaccaro D, Cuccarolo P, Columbaro M, Capanni C, Bartolucci M, Panfoli I, Morelli A, Dufour C, Cappelli E, Degan P
Biochimie95(10)1828-37(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The gene signature of OPA1, CTSA, NDUFA1, STK10 and PRDX1 was able to identify patients post-implant with a sensitivity of 91% and a specificity of 86% in discrimination between post-implant group and healthy controls.
Title: Altered mitochondrial expression genes in patients receiving right ventricular apical pacing.

Fanconi anemia complementation group A mutants show defective respiration through Complex I, diminished ATP production and metabolic sufferance with an increased AMP/ATP ratio.
Title: Mitochondrial respiratory chain Complex I defects in Fanconi anemia complementation group A.

Heterozygous mutation in the X chromosomal NDUFA1 gene in a girl with complex I deficiency
Title: Heterozygous mutation in the X chromosomal NDUFA1 gene in a girl with complex I deficiency.

Mutations in the NDUFA1 gene is linked to a delayed mitochondrial network recovery in OXPHOS disorders.
Title: Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects.

The following NDUFA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18506	NM_004541.4 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18506	NM_004541.3	Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFA1 ( NM_004541.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004532.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.

Price & Availability↑

CloneID	OHu18506
Clone ID Related Accession (Same CDS sequence)	NM_004541.4 , NM_004541.3
Accession Version	NM_004541.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002477.1 and BC000266.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004541.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.187689.1, AV682711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000371437.5/ ENSP00000360492.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGTTCG AGATTCTCCC CGGACTCTCC GTCATGGGCG TGTGCTTGTT GATTCCAGGA 
CTGGCTACTG CGTACATCCA CAGGTTCACT AACGGGGGCA AGGAAAAAAG GGTTGCTCAT 
TTTGGGTATC ACTGGAGTCT GATGGAAAGA GATAGGCGCA TCTCTGGAGT TGATCGTTAC 
TATGTGTCAA AGGGTTTGGA GAACATTGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004532.1
CDS	81..293
Translation	MWFEILPGLSVMGVCLLIPGLATAYIHRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKGLENID

Target ORF information:

RefSeq Version	NM_004541.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004541.4

ORF Insert Sequence:

ATGTGGTTCG AGATTCTCCC CGGACTCTCC GTCATGGGCG TGTGCTTGTT GATTCCAGGA 
CTGGCTACTG CGTACATCCA CAGGTTCACT AACGGGGGCA AGGAAAAAAG GGTTGCTCAT 
TTTGGGTATC ACTGGAGTCT GATGGAAAGA GATAGGCGCA TCTCTGGAGT TGATCGTTAC 
TATGTGTCAA AGGGTTTGGA GAACATTGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA1 ( NM_004541.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004532.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.

Price & Availability↑

CloneID	OHu18506
Clone ID Related Accession (Same CDS sequence)	NM_004541.4 , NM_004541.3
Accession Version	NM_004541.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002477.1, BC000266.1 and BQ680674.1. This sequence is a reference standard in the RefSeqGene project. On Nov 30, 2007 this sequence version replaced NM_004541.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.64547.1, SRR1163657.194471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGTTCG AGATTCTCCC CGGACTCTCC GTCATGGGCG TGTGCTTGTT GATTCCAGGA 
CTGGCTACTG CGTACATCCA CAGGTTCACT AACGGGGGCA AGGAAAAAAG GGTTGCTCAT 
TTTGGGTATC ACTGGAGTCT GATGGAAAGA GATAGGCGCA TCTCTGGAGT TGATCGTTAC 
TATGTGTCAA AGGGTTTGGA GAACATTGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004532.1
CDS	142..354
Translation	MWFEILPGLSVMGVCLLIPGLATAYIHRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKGLENID

Target ORF information:

RefSeq Version	NM_004541.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A1 (NDUFA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004541.3

ORF Insert Sequence:

ATGTGGTTCG AGATTCTCCC CGGACTCTCC GTCATGGGCG TGTGCTTGTT GATTCCAGGA 
CTGGCTACTG CGTACATCCA CAGGTTCACT AACGGGGGCA AGGAAAAAAG GGTTGCTCAT 
TTTGGGTATC ACTGGAGTCT GATGGAAAGA GATAGGCGCA TCTCTGGAGT TGATCGTTAC 
TATGTGTCAA AGGGTTTGGA GAACATTGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Altered mitochondrial expression genes in patients receiving right ventricular apical pacing.
Experimental and molecular pathology100(3)469-75(2016 06)
Xu H,Gao J,Wang F

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
PLoS genetics11(8)e1005428(2015 Aug)
Hasegawa Y,Taylor D,Ovchinnikov DA,Wolvetang EJ,de Torrent? L,Mar JC

Mitochondrial respiratory chain Complex I defects in Fanconi anemia complementation group A.
Biochimie95(10)1828-37(2013 Oct)
Ravera S,Vaccaro D,Cuccarolo P,Columbaro M,Capanni C,Bartolucci M,Panfoli I,Morelli A,Dufour C,Cappelli E,Degan P

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NDUFA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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