NDN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDN
Entrez Gene ID	4692
Full Name	necdin, MAGE family member
Synonyms	HsT16328,PWCR
General protein information	Preferred Names necdin, MAGE family member Names necdin Prader-Willi syndrome chromosome region necdin homolog necdin, melanoma antigen (MAGE) family member necdin-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q11.2
Summary	This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008].
Disorder MIM:	602117
Disorder Html:	Prader-Willi syndrome, 176270 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002487.3	NP_002478.1	necdin
NM_002487.2	NP_002478.1	necdin

Pathways from BioSystems

Homologies

	NDN	NP_001165573.1
Rattus norvegicus (Norway rat)	Ndn	NP_001008558.1
Bos taurus (cattle)	NDN	NP_001014982.1
Mus musculus (house mouse)	Ndn	NP_035012.2
Canis lupus familiaris (dog)	NDN	XP_005618297.1
Homo sapiens (human)	NDN	NP_002478.1
Pan troglodytes (chimpanzee)	NDN	XP_510257.2

Gene Ontology
Bibliography

Related articles in PubMed

A genetic locus for paranoia.
Crespi B, Read S, Salminen I, Hurd P
Biology letters14(1)(2018 Jan)

Hypermethylation of NDN promotes cell proliferation by activating the Wnt signaling pathway in colorectal cancer.
Hu YH, Chen Q, Lu YX, Zhang JM, Lin C, Zhang F, Zhang WJ, Li XM, Zhang W, Li XN
Oncotarget8(28)46191-46203(2017 Jul)

Exome analysis of Smith-Magenis-like syndrome cohort identifies de novo likely pathogenic variants.
Berger SI, Ciccone C, Simon KL, Malicdan MC, Vilboux T, Billington C, Fischer R, Introne WJ, Gropman A, Blancato JK, Mullikin JC, , Gahl WA, Huizing M, Smith ACM
Human genetics136(4)409-420(2017 Apr)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

One candidate variant was located in an alpha helix of Necdin (NDN), phased to the paternally inherited allele. NDN is maternally imprinted within the 15q11.2 Prader-Willi Syndrome (PWS) region
Title: Exome analysis of Smith-Magenis-like syndrome cohort identifies de novo likely pathogenic variants.

NDN is an imprinted tumor suppressor gene which affects cancer cell motility, invasion and growth and that its loss of function in ovarian cancer can be caused by both genetic and epigenetic mechanisms.
Title: NDN is an imprinted tumor suppressor gene that is downregulated in ovarian cancers through genetic and epigenetic mechanisms.

NDN and CD1A are novel prognostic methylation markers in patients with head and neck squamous carcinomas
Title: NDN and CD1A are novel prognostic methylation markers in patients with head and neck squamous carcinomas.

Germline single nucleotide polymorphism in necdin gene is associated with breast cancer.
Title: Necdin is a breast cancer metastasis suppressor that regulates the transcription of c-Myc.

Hypermethylation and mutation of necdin is associated with neoplasms.
Title: Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.

The following NDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19521	NM_002487.3 Latest version!	Homo sapiens necdin, MAGE family member (NDN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19521	NM_002487.2	Homo sapiens necdin, MAGE family member (NDN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDN ( NM_002487.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002478.1 Homo sapiens necdin, MAGE family member (NDN), mRNA.

Price & Availability↑

CloneID	OHu19521
Clone ID Related Accession (Same CDS sequence)	NM_002487.3 , NM_002487.2
Accession Version	NM_002487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	necdin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124309.7. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_002487.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR3476690.434425.1, DRR138527.509882.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9302265 MANE Ensembl match :: ENST00000649030.2/ ENSP00000497916.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC CCTAACTTTG CAGCCGAGGC CCCCAACTCC 
GAGGTGCACA GCAGCCCTGG GGTTTCGGAG GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG 
CCGCAGAGCC CTCCTCTAGG CCCGACGGCC GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC 
CCGAACGACG AGGGCGACCC GAAGGCCCTG CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC 
CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG GCACCGCCAG CCCCGGCGCA GCTGGTGCAG 
AAGGCGCACG AGCTCATGTG GTACGTGCTG GTCAAGGACC AGAAGAAGAT GATCATCTGG 
TTTCCAGACA TGGTGAAAGA TGTCATCGGC AGCTACAAGA AGTGGTGCAG GAGCATCCTC 
CGGCGCACCA GCCTCATCCT CGCCCGGGTG TTCGGGCTGC ACCTGAGGCT AACCAGCCTG 
CACACCATGG AGTTTGCGCT GGTCAAAGCG CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG 
CTGAGCAACC GCATGCCCAT GACAGGCCTC CTGCTCATGA TCCTGAGCCT CATCTACGTG 
AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC TGGAACGTGC TGCGCATCCT GGGGCTGCGG 
CCCTGGAAGA AGCACTCCAC CTTCGGGGAC GTGCGGAAGC TCATCACTGA GGAGTTCGTC 
CAAATGAATT ACCTGAAGTA CCAGCGCGTC CCATACGTGG AGCCGCCCGA ATACGAGTTC 
TTTTGGGGCT CCCGGGCCAG CCGCGAAATC ACCAAGATGC AAATCATGGA GTTCCTGGCC 
AGGGTCTTTA AGAAAGACCC CCAGGCCTGG CCCTCCCGAT ACAGAGAAGC TCTGGAGGAG 
GCCAGAGCTC TGCGGGAGGC TAATCCCACT GCCCACTACC CTCGCAGCAG TGTCTCTGAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002478.1
CDS	89..1054
Translation	MSEQSKDLSDPNFAAEAPNSEVHSSPGVSEGVPPSATLAEPQSPPLGPTAAPQAAPPPQAPNDEGDPKALQQAAEEGRAHQAPSAAQPGPAPPAPAQLVQKAHELMWYVLVKDQKKMIIWFPDMVKDVIGSYKKWCRSILRRTSLILARVFGLHLRLTSLHTMEFALVKALEPEELDRVALSNRMPMTGLLLMILSLIYVKGRGARESAVWNVLRILGLRPWKKHSTFGDVRKLITEEFVQMNYLKYQRVPYVEPPEYEFFWGSRASREITKMQIMEFLARVFKKDPQAWPSRYREALEEARALREANPTAHYPRSSVSED

Target ORF information:

RefSeq Version	NM_002487.3
Organism	Homo sapiens(human)
Definition	Homo sapiens necdin, MAGE family member (NDN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002487.3

ORF Insert Sequence:

ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC CCTAACTTTG CAGCCGAGGC CCCCAACTCC 
GAGGTGCACA GCAGCCCTGG GGTTTCGGAG GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG 
CCGCAGAGCC CTCCTCTAGG CCCGACGGCC GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC 
CCGAACGACG AGGGCGACCC GAAGGCCCTG CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC 
CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG GCACCGCCAG CCCCGGCGCA GCTGGTGCAG 
AAGGCGCACG AGCTCATGTG GTACGTGCTG GTCAAGGACC AGAAGAAGAT GATCATCTGG 
TTTCCAGACA TGGTGAAAGA TGTCATCGGC AGCTACAAGA AGTGGTGCAG GAGCATCCTC 
CGGCGCACCA GCCTCATCCT CGCCCGGGTG TTCGGGCTGC ACCTGAGGCT AACCAGCCTG 
CACACCATGG AGTTTGCGCT GGTCAAAGCG CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG 
CTGAGCAACC GCATGCCCAT GACAGGCCTC CTGCTCATGA TCCTGAGCCT CATCTACGTG 
AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC TGGAACGTGC TGCGCATCCT GGGGCTGCGG 
CCCTGGAAGA AGCACTCCAC CTTCGGGGAC GTGCGGAAGC TCATCACTGA GGAGTTCGTC 
CAAATGAATT ACCTGAAGTA CCAGCGCGTC CCATACGTGG AGCCGCCCGA ATACGAGTTC 
TTTTGGGGCT CCCGGGCCAG CCGCGAAATC ACCAAGATGC AAATCATGGA GTTCCTGGCC 
AGGGTCTTTA AGAAAGACCC CCAGGCCTGG CCCTCCCGAT ACAGAGAAGC TCTGGAGGAG 
GCCAGAGCTC TGCGGGAGGC TAATCCCACT GCCCACTACC CTCGCAGCAG TGTCTCTGAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDN ( NM_002487.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002478.1 Homo sapiens necdin, MAGE family member (NDN), mRNA.

Price & Availability↑

CloneID	OHu19521
Clone ID Related Accession (Same CDS sequence)	NM_002487.3 , NM_002487.2
Accession Version	NM_002487.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-18
Organism	Homo sapiens(human)
Product	necdin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB007828.1. This sequence is a reference standard in the RefSeqGene project. On Oct 13, 2000 this sequence version replaced NM_002487.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 9302265 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC CCTAACTTTG CAGCCGAGGC CCCCAACTCC 
GAGGTGCACA GCAGCCCTGG GGTTTCGGAG GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG 
CCGCAGAGCC CTCCTCTAGG CCCGACGGCC GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC 
CCGAACGACG AGGGCGACCC GAAGGCCCTG CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC 
CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG GCACCGCCAG CCCCGGCGCA GCTGGTGCAG 
AAGGCGCACG AGCTCATGTG GTACGTGCTG GTCAAGGACC AGAAGAAGAT GATCATCTGG 
TTTCCAGACA TGGTGAAAGA TGTCATCGGC AGCTACAAGA AGTGGTGCAG GAGCATCCTC 
CGGCGCACCA GCCTCATCCT CGCCCGGGTG TTCGGGCTGC ACCTGAGGCT AACCAGCCTG 
CACACCATGG AGTTTGCGCT GGTCAAAGCG CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG 
CTGAGCAACC GCATGCCCAT GACAGGCCTC CTGCTCATGA TCCTGAGCCT CATCTACGTG 
AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC TGGAACGTGC TGCGCATCCT GGGGCTGCGG 
CCCTGGAAGA AGCACTCCAC CTTCGGGGAC GTGCGGAAGC TCATCACTGA GGAGTTCGTC 
CAAATGAATT ACCTGAAGTA CCAGCGCGTC CCATACGTGG AGCCGCCCGA ATACGAGTTC 
TTTTGGGGCT CCCGGGCCAG CCGCGAAATC ACCAAGATGC AAATCATGGA GTTCCTGGCC 
AGGGTCTTTA AGAAAGACCC CCAGGCCTGG CCCTCCCGAT ACAGAGAAGC TCTGGAGGAG 
GCCAGAGCTC TGCGGGAGGC TAATCCCACT GCCCACTACC CTCGCAGCAG TGTCTCTGAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002478.1
CDS	87..1052
Translation	MSEQSKDLSDPNFAAEAPNSEVHSSPGVSEGVPPSATLAEPQSPPLGPTAAPQAAPPPQAPNDEGDPKALQQAAEEGRAHQAPSAAQPGPAPPAPAQLVQKAHELMWYVLVKDQKKMIIWFPDMVKDVIGSYKKWCRSILRRTSLILARVFGLHLRLTSLHTMEFALVKALEPEELDRVALSNRMPMTGLLLMILSLIYVKGRGARESAVWNVLRILGLRPWKKHSTFGDVRKLITEEFVQMNYLKYQRVPYVEPPEYEFFWGSRASREITKMQIMEFLARVFKKDPQAWPSRYREALEEARALREANPTAHYPRSSVSED

Target ORF information:

RefSeq Version	NM_002487.2
Organism	Homo sapiens(human)
Definition	Homo sapiens necdin, MAGE family member (NDN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002487.2

ORF Insert Sequence:

ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC CCTAACTTTG CAGCCGAGGC CCCCAACTCC 
GAGGTGCACA GCAGCCCTGG GGTTTCGGAG GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG 
CCGCAGAGCC CTCCTCTAGG CCCGACGGCC GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC 
CCGAACGACG AGGGCGACCC GAAGGCCCTG CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC 
CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG GCACCGCCAG CCCCGGCGCA GCTGGTGCAG 
AAGGCGCACG AGCTCATGTG GTACGTGCTG GTCAAGGACC AGAAGAAGAT GATCATCTGG 
TTTCCAGACA TGGTGAAAGA TGTCATCGGC AGCTACAAGA AGTGGTGCAG GAGCATCCTC 
CGGCGCACCA GCCTCATCCT CGCCCGGGTG TTCGGGCTGC ACCTGAGGCT AACCAGCCTG 
CACACCATGG AGTTTGCGCT GGTCAAAGCG CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG 
CTGAGCAACC GCATGCCCAT GACAGGCCTC CTGCTCATGA TCCTGAGCCT CATCTACGTG 
AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC TGGAACGTGC TGCGCATCCT GGGGCTGCGG 
CCCTGGAAGA AGCACTCCAC CTTCGGGGAC GTGCGGAAGC TCATCACTGA GGAGTTCGTC 
CAAATGAATT ACCTGAAGTA CCAGCGCGTC CCATACGTGG AGCCGCCCGA ATACGAGTTC 
TTTTGGGGCT CCCGGGCCAG CCGCGAAATC ACCAAGATGC AAATCATGGA GTTCCTGGCC 
AGGGTCTTTA AGAAAGACCC CCAGGCCTGG CCCTCCCGAT ACAGAGAAGC TCTGGAGGAG 
GCCAGAGCTC TGCGGGAGGC TAATCCCACT GCCCACTACC CTCGCAGCAG TGTCTCTGAG 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A genetic locus for paranoia.
Biology letters14(1)(2018 Jan)
Crespi B,Read S,Salminen I,Hurd P

Hypermethylation of NDN promotes cell proliferation by activating the Wnt signaling pathway in colorectal cancer.
Oncotarget8(28)46191-46203(2017 Jul)
Hu YH,Chen Q,Lu YX,Zhang JM,Lin C,Zhang F,Zhang WJ,Li XM,Zhang W,Li XN

Exome analysis of Smith-Magenis-like syndrome cohort identifies de novo likely pathogenic variants.
Human genetics136(4)409-420(2017 Apr)
Berger SI,Ciccone C,Simon KL,Malicdan MC,Vilboux T,Billington C,Fischer R,Introne WJ,Gropman A,Blancato JK,Mullikin JC,,Gahl WA,Huizing M,Smith ACM

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

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NDN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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