NCK1 cDNA ORF clone, Homo sapiens(human)

The following NCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24619 NM_006153.6
Latest version!
Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu24619 NM_001291999.2
Latest version!
Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu24619 XM_024453538.1
Latest version!
Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu24620 NM_001190796.3
Latest version!
Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu24619 NM_001291999.1 Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu24620 NM_001190796.2 Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu24619 NM_006153.5 Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24619
    Clone ID Related Accession (Same CDS sequence) XM_024453538.1 , NM_001291999.2 , NM_001291999.1 , NM_006153.5 , NM_006153.6
    Accession Version NM_001291999.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA855124.1, BC006403.2 and AC011597.27. On Nov 23, 2018 this sequence version replaced NM_001291999.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.254690.1, SRR1660807.77761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000481752.6/ ENSP00000417273.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278928.1
    CDS165..1298
    Translation

    Target ORF information:

    RefSeq Version NM_001291999.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291999.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24619
    Clone ID Related Accession (Same CDS sequence) XM_024453538.1 , NM_001291999.2 , NM_001291999.1 , NM_006153.5 , NM_006153.6
    Accession Version NM_001291999.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA855124.1, BC006403.2 and AC011597.27. On May 15, 2014 this sequence version replaced XM_005247498.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.254690.1, SRR1660807.77761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278928.1
    CDS165..1298
    Translation

    Target ORF information:

    RefSeq Version NM_001291999.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24619
    Clone ID Related Accession (Same CDS sequence) XM_024453538.1 , NM_001291999.2 , NM_001291999.1 , NM_006153.5 , NM_006153.6
    Accession Version NM_006153.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB117533.1, BC006403.2 and AC011597.27. On May 15, 2014 this sequence version replaced NM_006153.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.136471.1, SRR1660805.254761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006144.1
    CDS131..1264
    Translation

    Target ORF information:

    RefSeq Version NM_006153.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006153.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24619
    Clone ID Related Accession (Same CDS sequence) XM_024453538.1 , NM_001291999.2 , NM_001291999.1 , NM_006153.5 , NM_006153.6
    Accession Version NM_006153.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB117533.1, BC006403.2 and AC011597.27. On May 31, 2019 this sequence version replaced NM_006153.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.136471.1, SRR1803614.183094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006144.1
    CDS131..1264
    Translation

    Target ORF information:

    RefSeq Version NM_006153.6
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006153.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24619
    Clone ID Related Accession (Same CDS sequence) XM_024453538.1 , NM_001291999.2 , NM_001291999.1 , NM_006153.5 , NM_006153.6
    Accession Version XM_024453538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309306.1
    CDS275..1408
    Translation

    Target ORF information:

    RefSeq Version XM_024453538.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAAG AAGTGGTGGT AGTAGCCAAA TTTGATTATG TGGCCCAACA AGAACAAGAG 
    TTGGACATCA AGAAGAATGA GAGATTATGG CTTCTGGATG ATTCTAAGTC CTGGTGGCGA
    GTTCGAAATT CCATGAATAA AACAGGTTTT GTGCCTTCTA ACTATGTGGA AAGGAAAAAC
    AGTGCTCGGA AAGCATCTAT TGTGAAAAAC CTAAAGGATA CCTTAGGCAT TGGAAAAGTG
    AAAAGAAAAC CTAGTGTGCC AGATTCTGCA TCTCCTGCTG ATGATAGTTT TGTTGACCCA
    GGGGAACGTC TCTATGACCT CAACATGCCC GCTTATGTGA AATTTAACTA CATGGCTGAG
    AGAGAGGATG AATTATCATT GATAAAGGGG ACAAAGGTGA TCGTCATGGA GAAATGCAGT
    GATGGGTGGT GGCGTGGTAG CTACAATGGA CAAGTTGGAT GGTTCCCTTC AAACTATGTA
    ACTGAAGAAG GTGACAGTCC TTTGGGTGAC CATGTGGGTT CTCTGTCAGA GAAATTAGCA
    GCAGTCGTCA ATAACCTAAA TACTGGGCAA GTGTTGCATG TGGTACAGGC TCTTTACCCA
    TTCAGCTCAT CTAATGATGA AGAACTTAAT TTCGAGAAAG GAGATGTAAT GGATGTTATT
    GAAAAACCTG AAAATGACCC AGAGTGGTGG AAATGCAGGA AGATCAATGG TATGGTTGGT
    CTAGTACCAA AAAACTATGT TACCGTTATG CAGAATAATC CATTAACTTC AGGTTTGGAA
    CCATCACCTC CACAGTGTGA TTACATTAGG CCTTCACTCA CTGGAAAGTT TGCTGGCAAT
    CCTTGGTATT ATGGCAAAGT CACCAGGCAT CAAGCAGAAA TGGCATTAAA TGAAAGAGGA
    CATGAAGGGG ATTTCCTCAT TCGTGATAGT GAATCTTCGC CAAATGATTT CTCAGTATCA
    CTAAAAGCAC AAGGGAAAAA CAAGCATTTT AAAGTCCAAC TAAAAGAGAC TGTCTACTGC
    ATTGGGCAGC GTAAATTCAG CACCATGGAA GAACTTGTAG AACATTACAA AAAGGCACCA
    ATTTTTACAA GTGAACAAGG AGAAAAATTA TATCTTGTCA AGCATTTATC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24620
    Clone ID Related Accession (Same CDS sequence) NM_001190796.2 , NM_001190796.3
    Accession Version NM_001190796.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP339594.1, AK301460.1, BC006403.2 and AC011597.27. On May 15, 2014 this sequence version replaced NM_001190796.1. Transcript Variant: This variant (2) has an alternate 5' sequence compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.228386.1, ERR279870.546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTGGT TAAACGTCTT TAAAGATTTT TTCAGCATTG GAAAAGTGAA AAGAAAACCT 
    AGTGTGCCAG ATTCTGCATC TCCTGCTGAT GATAGTTTTG TTGACCCAGG GGAACGTCTC
    TATGACCTCA ACATGCCCGC TTATGTGAAA TTTAACTACA TGGCTGAGAG AGAGGATGAA
    TTATCATTGA TAAAGGGGAC AAAGGTGATC GTCATGGAGA AATGCAGTGA TGGGTGGTGG
    CGTGGTAGCT ACAATGGACA AGTTGGATGG TTCCCTTCAA ACTATGTAAC TGAAGAAGGT
    GACAGTCCTT TGGGTGACCA TGTGGGTTCT CTGTCAGAGA AATTAGCAGC AGTCGTCAAT
    AACCTAAATA CTGGGCAAGT GTTGCATGTG GTACAGGCTC TTTACCCATT CAGCTCATCT
    AATGATGAAG AACTTAATTT CGAGAAAGGA GATGTAATGG ATGTTATTGA AAAACCTGAA
    AATGACCCAG AGTGGTGGAA ATGCAGGAAG ATCAATGGTA TGGTTGGTCT AGTACCAAAA
    AACTATGTTA CCGTTATGCA GAATAATCCA TTAACTTCAG GTTTGGAACC ATCACCTCCA
    CAGTGTGATT ACATTAGGCC TTCACTCACT GGAAAGTTTG CTGGCAATCC TTGGTATTAT
    GGCAAAGTCA CCAGGCATCA AGCAGAAATG GCATTAAATG AAAGAGGACA TGAAGGGGAT
    TTCCTCATTC GTGATAGTGA ATCTTCGCCA AATGATTTCT CAGTATCACT AAAAGCACAA
    GGGAAAAACA AGCATTTTAA AGTCCAACTA AAAGAGACTG TCTACTGCAT TGGGCAGCGT
    AAATTCAGCA CCATGGAAGA ACTTGTAGAA CATTACAAAA AGGCACCAAT TTTTACAAGT
    GAACAAGGAG AAAAATTATA TCTTGTCAAG CATTTATCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177725.1
    CDS87..1028
    Translation

    Target ORF information:

    RefSeq Version NM_001190796.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190796.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTGGT TAAACGTCTT TAAAGATTTT TTCAGCATTG GAAAAGTGAA AAGAAAACCT 
    AGTGTGCCAG ATTCTGCATC TCCTGCTGAT GATAGTTTTG TTGACCCAGG GGAACGTCTC
    TATGACCTCA ACATGCCCGC TTATGTGAAA TTTAACTACA TGGCTGAGAG AGAGGATGAA
    TTATCATTGA TAAAGGGGAC AAAGGTGATC GTCATGGAGA AATGCAGTGA TGGGTGGTGG
    CGTGGTAGCT ACAATGGACA AGTTGGATGG TTCCCTTCAA ACTATGTAAC TGAAGAAGGT
    GACAGTCCTT TGGGTGACCA TGTGGGTTCT CTGTCAGAGA AATTAGCAGC AGTCGTCAAT
    AACCTAAATA CTGGGCAAGT GTTGCATGTG GTACAGGCTC TTTACCCATT CAGCTCATCT
    AATGATGAAG AACTTAATTT CGAGAAAGGA GATGTAATGG ATGTTATTGA AAAACCTGAA
    AATGACCCAG AGTGGTGGAA ATGCAGGAAG ATCAATGGTA TGGTTGGTCT AGTACCAAAA
    AACTATGTTA CCGTTATGCA GAATAATCCA TTAACTTCAG GTTTGGAACC ATCACCTCCA
    CAGTGTGATT ACATTAGGCC TTCACTCACT GGAAAGTTTG CTGGCAATCC TTGGTATTAT
    GGCAAAGTCA CCAGGCATCA AGCAGAAATG GCATTAAATG AAAGAGGACA TGAAGGGGAT
    TTCCTCATTC GTGATAGTGA ATCTTCGCCA AATGATTTCT CAGTATCACT AAAAGCACAA
    GGGAAAAACA AGCATTTTAA AGTCCAACTA AAAGAGACTG TCTACTGCAT TGGGCAGCGT
    AAATTCAGCA CCATGGAAGA ACTTGTAGAA CATTACAAAA AGGCACCAAT TTTTACAAGT
    GAACAAGGAG AAAAATTATA TCTTGTCAAG CATTTATCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24620
    Clone ID Related Accession (Same CDS sequence) NM_001190796.2 , NM_001190796.3
    Accession Version NM_001190796.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product cytoplasmic protein NCK1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP339594.1, AK301460.1, BC006403.2 and AC011597.27. On May 31, 2019 this sequence version replaced NM_001190796.2. Transcript Variant: This variant (2) has an alternate 5' sequence compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.228386.1, SRR5189655.136949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTGGT TAAACGTCTT TAAAGATTTT TTCAGCATTG GAAAAGTGAA AAGAAAACCT 
    AGTGTGCCAG ATTCTGCATC TCCTGCTGAT GATAGTTTTG TTGACCCAGG GGAACGTCTC
    TATGACCTCA ACATGCCCGC TTATGTGAAA TTTAACTACA TGGCTGAGAG AGAGGATGAA
    TTATCATTGA TAAAGGGGAC AAAGGTGATC GTCATGGAGA AATGCAGTGA TGGGTGGTGG
    CGTGGTAGCT ACAATGGACA AGTTGGATGG TTCCCTTCAA ACTATGTAAC TGAAGAAGGT
    GACAGTCCTT TGGGTGACCA TGTGGGTTCT CTGTCAGAGA AATTAGCAGC AGTCGTCAAT
    AACCTAAATA CTGGGCAAGT GTTGCATGTG GTACAGGCTC TTTACCCATT CAGCTCATCT
    AATGATGAAG AACTTAATTT CGAGAAAGGA GATGTAATGG ATGTTATTGA AAAACCTGAA
    AATGACCCAG AGTGGTGGAA ATGCAGGAAG ATCAATGGTA TGGTTGGTCT AGTACCAAAA
    AACTATGTTA CCGTTATGCA GAATAATCCA TTAACTTCAG GTTTGGAACC ATCACCTCCA
    CAGTGTGATT ACATTAGGCC TTCACTCACT GGAAAGTTTG CTGGCAATCC TTGGTATTAT
    GGCAAAGTCA CCAGGCATCA AGCAGAAATG GCATTAAATG AAAGAGGACA TGAAGGGGAT
    TTCCTCATTC GTGATAGTGA ATCTTCGCCA AATGATTTCT CAGTATCACT AAAAGCACAA
    GGGAAAAACA AGCATTTTAA AGTCCAACTA AAAGAGACTG TCTACTGCAT TGGGCAGCGT
    AAATTCAGCA CCATGGAAGA ACTTGTAGAA CATTACAAAA AGGCACCAAT TTTTACAAGT
    GAACAAGGAG AAAAATTATA TCTTGTCAAG CATTTATCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177725.1
    CDS60..1001
    Translation

    Target ORF information:

    RefSeq Version NM_001190796.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190796.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTGGT TAAACGTCTT TAAAGATTTT TTCAGCATTG GAAAAGTGAA AAGAAAACCT 
    AGTGTGCCAG ATTCTGCATC TCCTGCTGAT GATAGTTTTG TTGACCCAGG GGAACGTCTC
    TATGACCTCA ACATGCCCGC TTATGTGAAA TTTAACTACA TGGCTGAGAG AGAGGATGAA
    TTATCATTGA TAAAGGGGAC AAAGGTGATC GTCATGGAGA AATGCAGTGA TGGGTGGTGG
    CGTGGTAGCT ACAATGGACA AGTTGGATGG TTCCCTTCAA ACTATGTAAC TGAAGAAGGT
    GACAGTCCTT TGGGTGACCA TGTGGGTTCT CTGTCAGAGA AATTAGCAGC AGTCGTCAAT
    AACCTAAATA CTGGGCAAGT GTTGCATGTG GTACAGGCTC TTTACCCATT CAGCTCATCT
    AATGATGAAG AACTTAATTT CGAGAAAGGA GATGTAATGG ATGTTATTGA AAAACCTGAA
    AATGACCCAG AGTGGTGGAA ATGCAGGAAG ATCAATGGTA TGGTTGGTCT AGTACCAAAA
    AACTATGTTA CCGTTATGCA GAATAATCCA TTAACTTCAG GTTTGGAACC ATCACCTCCA
    CAGTGTGATT ACATTAGGCC TTCACTCACT GGAAAGTTTG CTGGCAATCC TTGGTATTAT
    GGCAAAGTCA CCAGGCATCA AGCAGAAATG GCATTAAATG AAAGAGGACA TGAAGGGGAT
    TTCCTCATTC GTGATAGTGA ATCTTCGCCA AATGATTTCT CAGTATCACT AAAAGCACAA
    GGGAAAAACA AGCATTTTAA AGTCCAACTA AAAGAGACTG TCTACTGCAT TGGGCAGCGT
    AAATTCAGCA CCATGGAAGA ACTTGTAGAA CATTACAAAA AGGCACCAAT TTTTACAAGT
    GAACAAGGAG AAAAATTATA TCTTGTCAAG CATTTATCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.