NCF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCF4
Entrez Gene ID	4689
Full Name	neutrophil cytosolic factor 4
Synonyms	CGD3,NCF,P40PHOX,SH3PXD4
General protein information	Preferred Names neutrophil cytosolic factor 4 Names neutrophil cytosol factor 4 NCF-4 SH3 and PX domain-containing protein 4 neutrophil NADPH oxidase factor 4 neutrophil cytosolic factor 4, 40kDa p40-phox
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.3
Summary	The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Disorder MIM:	601488
Disorder Html:	?Granulomatous disease, chronic, autosomal recessive, cytochrome b-positive, type III, 613960 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000631.4	NP_000622.2	neutrophil cytosol factor 4 isoform 1
NM_013416.3	NP_038202.2	neutrophil cytosol factor 4 isoform 2
NM_000631.5	NP_000622.2	neutrophil cytosol factor 4 isoform 1
XM_017028808.1	XP_016884297.1	neutrophil cytosol factor 4 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NCF4	NP_001034361.1
Xenopus tropicalis (tropical clawed frog)	ncf4	NP_001025692.1
Mus musculus (house mouse)	Ncf4	NP_032703.2
Homo sapiens (human)	NCF4	NP_000622.2
Rattus norvegicus (Norway rat)	Ncf4	NP_001120776.1
Danio rerio (zebrafish)	ncf4	NP_991274.2
Pan troglodytes (chimpanzee)	NCF4	XP_001159332.2
Canis lupus familiaris (dog)	NCF4	XP_538398.2
Bos taurus (cattle)	NCF4	NP_001039448.1

Gene Ontology
Bibliography

Related articles in PubMed

Tks adaptor proteins at a glance.
Saini P, Courtneidge SA
Journal of cell science131(1)(2018 Jan)

Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome.
Song ZM, Bouchab L, Hudik E, Le Bars R, N?sse O, Dupr?-Crochet S
Journal of leukocyte biology101(5)1155-1168(2017 05)

Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.
Wu PB, Dai JF, Wang Q, Zhang G, Tan SY, Li M, Ye HL
Inflammation research : official journal of the European Histamine Research Society ... [et al.]64(10)825-31(2015 Oct)

Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.
Kim YM, Cho M
Cellular signalling26(4)784-96(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane.
Title: Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome.

NCF4 polymorphism was associated with Crohn's disease, but not ulcerative colitis in Caucasian--{REVIEW}
Title: Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.

NCF4 may induce ex- pression of NADPH oxidase enzymes, such as p67phox, p47phox, p22phox and NOX2, which lead to increased ROS levels.
Title: Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.

Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.
Title: Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.

The contribution of the functionally relevant NADPH polymorphisms rs1883112 and rs4673 to anthracycline-related heart lesions provides a plausible explanation for their modulation of cardiotoxicity.
Title: Association of anthracycline-related cardiac histological lesions with NADPH oxidase functional polymorphisms.

The following NCF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16465	NM_000631.5 Latest version!	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu18835	NM_013416.3 Latest version!	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu93501	XM_017028808.1 Latest version!	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu16465	NM_000631.4	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCF4 ( NM_000631.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000622.2 Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16465
Clone ID Related Accession (Same CDS sequence)	NM_000631.4 , NM_000631.5
Accession Version	NM_000631.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	neutrophil cytosol factor 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, BX355636.2 and BU076892.1. On Dec 20, 2007 this sequence version replaced NM_000631.3. Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.191850.1, SRR1163657.573546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT 
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA 
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT 
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000622.2
CDS	185..1204
Translation	MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Target ORF information:

RefSeq Version	NM_000631.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000631.4

ORF Insert Sequence:

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT 
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA 
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT 
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCF4 ( NM_000631.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000622.2 Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16465
Clone ID Related Accession (Same CDS sequence)	NM_000631.4 , NM_000631.5
Accession Version	NM_000631.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	neutrophil cytosol factor 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, BX355636.2 and BU076892.1. On Nov 22, 2018 this sequence version replaced NM_000631.4. Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.936687.1, SRR3476690.958705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248899.11/ ENSP00000248899.6 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT 
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA 
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT 
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000622.2
CDS	167..1186
Translation	MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Target ORF information:

RefSeq Version	NM_000631.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000631.5

ORF Insert Sequence:

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT 
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA 
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT 
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCF4 ( NM_013416.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_038202.2 Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18835
Clone ID Related Accession (Same CDS sequence)	NM_013416.3
Accession Version	NM_013416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	neutrophil cytosol factor 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, AK290924.1 and BU076892.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced NM_013416.2. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002798.1, AK290924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG 
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG 
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC 
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC 
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG 
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038202.2
CDS	185..1231
Translation	MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKSVAWEGGACPAFLPSLRPLPLTSPSHGSLSHSKAPSGSQMSHNAVTSHQRPGWPGQPHSPFPHPTPHFQPDASLLQPVTPLGTSRWRKISAALPY

Target ORF information:

RefSeq Version	NM_013416.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013416.3

ORF Insert Sequence:

ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT 
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC 
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT 
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG 
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG 
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA 
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG 
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC 
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC 
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT 
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG 
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG 
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG 
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC 
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC 
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG 
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCF4 ( XM_017028808.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884297.1 Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu93501
Clone ID Related Accession (Same CDS sequence)	XM_017028808.1
Accession Version	XM_017028808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neutrophil cytosol factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGATAC CTGCCCTCAA CGCCTACATG AAGAGCCTGC TCAGCCTGCC GGTCTGGGTG 
CTGATGGATG AGGACGTCCG GATCTTCTTT TACCAGTCGC CCTATGACTC AGAGCAGGTG 
CCCCAGGCAC TCCGCCGGCT CCGCCCGCGC ACCCGGAAAG TCAAGAGCGT GTCCCCACAG 
GGCAACAGCG TTGACCGCAT GGCAGCTCCG AGAGCAGAGG CTCTATTTGA CTTCACTGGA 
AACAGCAAAC TGGAGCTGAA TTTCAAAGCT GGAGATGTGA TCTTCCTCCT CAGTCGGATC 
AACAAAGACT GGCTGGAGGG CACTGTCCGG GGAGCCACGG GCATCTTCCC TCTCTCCTTC 
GTGAAGATCC TCAAAGACTT CCCTGAGGAG GACGACCCCA CCAACTGGCT GCGTTGCTAC 
TACTACGAAG ACACCATCAG CACCATCAAG GACATCGCGG TGGAGGAAGA TCTCAGCAGC 
ACTCCCCTAT TGAAAGACCT GCTGGAGCTC ACAAGGCGGG AGTTCCAGAG AGAGGACATA 
GCTCTGAATT ACCGGGACGC TGAGGGGGAT CTGGTTCGGC TGCTGTCGGA TGAGGACGTA 
GCGCTCATGG TGCGGCAGGC TCGTGGCCTC CCCTCCCAGA AGCGCCTCTT CCCCTGGAAG 
CTGCACATCA CGCAGAAGGA CAACTACAGG GTCTACAACA CGATGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884297.1
CDS	858..1568
Translation	MRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Target ORF information:

RefSeq Version	XM_017028808.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028808.1

ORF Insert Sequence:

ATGCGGATAC CTGCCCTCAA CGCCTACATG AAGAGCCTGC TCAGCCTGCC GGTCTGGGTG 
CTGATGGATG AGGACGTCCG GATCTTCTTT TACCAGTCGC CCTATGACTC AGAGCAGGTG 
CCCCAGGCAC TCCGCCGGCT CCGCCCGCGC ACCCGGAAAG TCAAGAGCGT GTCCCCACAG 
GGCAACAGCG TTGACCGCAT GGCAGCTCCG AGAGCAGAGG CTCTATTTGA CTTCACTGGA 
AACAGCAAAC TGGAGCTGAA TTTCAAAGCT GGAGATGTGA TCTTCCTCCT CAGTCGGATC 
AACAAAGACT GGCTGGAGGG CACTGTCCGG GGAGCCACGG GCATCTTCCC TCTCTCCTTC 
GTGAAGATCC TCAAAGACTT CCCTGAGGAG GACGACCCCA CCAACTGGCT GCGTTGCTAC 
TACTACGAAG ACACCATCAG CACCATCAAG GACATCGCGG TGGAGGAAGA TCTCAGCAGC 
ACTCCCCTAT TGAAAGACCT GCTGGAGCTC ACAAGGCGGG AGTTCCAGAG AGAGGACATA 
GCTCTGAATT ACCGGGACGC TGAGGGGGAT CTGGTTCGGC TGCTGTCGGA TGAGGACGTA 
GCGCTCATGG TGCGGCAGGC TCGTGGCCTC CCCTCCCAGA AGCGCCTCTT CCCCTGGAAG 
CTGCACATCA CGCAGAAGGA CAACTACAGG GTCTACAACA CGATGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tks adaptor proteins at a glance.
Journal of cell science131(1)(2018 Jan)
Saini P,Courtneidge SA

Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome.
Journal of leukocyte biology101(5)1155-1168(2017 05)
Song ZM,Bouchab L,Hudik E,Le Bars R,N?sse O,Dupr?-Crochet S

Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]64(10)825-31(2015 Oct)
Wu PB,Dai JF,Wang Q,Zhang G,Tan SY,Li M,Ye HL

Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.
Cellular signalling26(4)784-96(2014 Apr)
Kim YM,Cho M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

NCF4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報